CN1102148C - Preparation method of scutellaria root extract - Google Patents
Preparation method of scutellaria root extract Download PDFInfo
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- CN1102148C CN1102148C CN00102536A CN00102536A CN1102148C CN 1102148 C CN1102148 C CN 1102148C CN 00102536 A CN00102536 A CN 00102536A CN 00102536 A CN00102536 A CN 00102536A CN 1102148 C CN1102148 C CN 1102148C
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- radix scutellariae
- preparation
- scutellariae extract
- ethanol
- resin
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Abstract
The present invention relates to the extraction of plant effective ingredients, particularly to a preparation method of scutellaria root extract. The scutellaria root extract has the following effective ingredients: scutellarin, wogonin and tetuin. The present invention is characterized in that after being pulverized, the scutellaria root is percolated and extracted by alcohol; after the alcohol is recycled by percolate, the alcohol is diluted by water; the chromatographic separation of an upper resin column is carried out; the upper resin column is eluted by water and organic solvents; then, organic solvent eluent is collected; the solvents are recycled, and finally, the scutellaria root extract is obtained. Compared with the prior art, the present invention has the following advantages: the adopted alcohol diacolation has easy technology and saved raw materials; the adopted resin extraction technology has high safety, and is suitable for industrialized production.
Description
The present invention relates to the preparation method of the extraction of active ingredients of plants, particularly Radix Scutellariae extract, the effective ingredient of this Radix Scutellariae extract is scutellarin, wogonin, thousand layers of fat element.
In the prior art, number of patent application 97109018.1 " being used for suppressing the medicament of uteri excitation " relates to the Radix Scutellariae extract extracting method, it is a raw material with the root of large-flowered skullcap, uses alcohol reflux, the reduction vaporization extracting solution, use extracted with diethyl ether then, the ether layer evaporated under reduced pressure must be made with extra care Radix Scutellariae total flavones, and the shortcoming of this extracting method is to be not suitable for suitability for industrialized production, one: adopt alcohol reflux in this technology, complex process, the ethanol consumption is big; Its two: this process using extracted with diethyl ether, ether is inflammable, explosive, industrial production is dangerous.
The object of the present invention is to provide a kind of preparation method who is suitable for the Radix Scutellariae extract of suitability for industrialized production.
For achieving the above object, the present invention adopts following technical scheme: the effective ingredient of described Radix Scutellariae extract is scutellarin, wogonin, thousand layers of fat element, after preparation method's root of large-flowered skullcap is pulverized, use ethanol percolate extraction, thin up behind the percolate recovery ethanol, last resin column chromatography for separation, water and organic solvent wash-out, collect the organic solvent elutriant, reclaim solvent, promptly get Radix Scutellariae extract.Wherein the alcohol concn that uses of diacolation is 30-95%; The resin that uses is polyamide resin, silica gel resin, ion exchange resin, macroporous adsorbent resin.The wash-out organic solvent is ethanol, methyl alcohol, acetone, chloroform, ethyl acetate, sherwood oil.
The present invention compared with prior art has following advantage: adopt ethanol percolation, technology is easy, saves starting material, and adopts the resin extraction process, and security is good, is more suitable for suitability for industrialized production.
The invention will be further described with embodiment below:
Embodiment 1:
Get root of large-flowered skullcap meal 15kg, use 95% ethanol percolate extraction, thin up behind the percolate recovery ethanol, use the polyamide resin column chromatography for separation, water → 50% ethanol → 95% ethanol elution successively, the aqueous solution discards, after 50% ethanol eluate and 95% elutriant reclaim ethanol respectively, the heating evaporate to dryness, merge alcohol eluen, remove solvent, promptly get Radix Scutellariae extract, about 450 grams, yield about 3%.
Embodiment 2:
Get root of large-flowered skullcap meal 15kg, use 80% ethanol percolate extraction, thin up behind the percolate recovery ethanol, with silica gel resin column chromatographic separation, water → methanol-eluted fractions successively, the aqueous solution discards, after elutriant reclaims methyl alcohol, the heating evaporate to dryness, get alcohol eluen, remove solvent, promptly get Radix Scutellariae extract, about 450 grams, yield about 3%.
Embodiment 3:
Get root of large-flowered skullcap meal 15kg, use 70% ethanol percolate extraction, thin up behind the percolate recovery ethanol, spent ion exchange resin column chromatography for separation, water → acetone wash-out successively, the aqueous solution discards, after elutriant reclaims acetone, and the heating evaporate to dryness, get elutriant, remove solvent, promptly get about 450 grams of Radix Scutellariae extract, yield about 3%.
Embodiment 4:
Get root of large-flowered skullcap meal 15kg, use 50% ethanol percolate extraction, thin up behind the percolate recovery ethanol is with D101 macropore absorption resin chromatographic separation, water → chloroform wash-out successively, the aqueous solution discards, after elutriant reclaims chloroform, and the heating evaporate to dryness, get elutriant, remove solvent, promptly get about 450 grams of Radix Scutellariae extract, yield about 3%.
Embodiment 5:
Get root of large-flowered skullcap meal 15kg, use 40% ethanol percolate extraction, dilute with water behind the percolate recovery ethanol is with silica gel resin column chromatographic separation, water → ethyl acetate wash-out successively, the aqueous solution discards, after elutriant reclaims ethyl acetate, and the heating evaporate to dryness, get elutriant, remove solvent, promptly get about 450 grams of Radix Scutellariae extract, yield about 3%.
Embodiment 6:
Get root of large-flowered skullcap meal 15kg, use 30% ethanol percolate extraction, dilute with water behind the percolate recovery ethanol is with silica gel resin column chromatographic separation, water → ethanol elution successively, the aqueous solution discards, after elutriant reclaims ethanol, and the heating evaporate to dryness, get elutriant, remove solvent, promptly get about 450 grams of Radix Scutellariae extract, yield about 3%.
Claims (4)
1, a kind of preparation method of Radix Scutellariae extract, the effective ingredient of described Radix Scutellariae extract is scutellarin, wogonin, thousand layers of fat element, it is characterized in that: after the root of large-flowered skullcap is pulverized, use ethanol percolate extraction, thin up behind the percolate recovery ethanol, last resin column chromatography for separation, water and organic solvent wash-out, collect the organic solvent elutriant, reclaim solvent, promptly get Radix Scutellariae extract.
2, the preparation method of Radix Scutellariae extract according to claim 1 is characterized in that: wherein the alcohol concn that uses of diacolation is 30-95%.
3, the preparation method of Radix Scutellariae extract according to claim 1 is characterized in that: the resin that uses is polyamide resin, silica gel resin, ion exchange resin, macroporous adsorbent resin.
4, the preparation method of Radix Scutellariae extract according to claim 1 is characterized in that: the wash-out organic solvent is ethanol, methyl alcohol, acetone, chloroform, ethyl acetate, sherwood oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00102536A CN1102148C (en) | 2000-03-03 | 2000-03-03 | Preparation method of scutellaria root extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00102536A CN1102148C (en) | 2000-03-03 | 2000-03-03 | Preparation method of scutellaria root extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1268512A CN1268512A (en) | 2000-10-04 |
| CN1102148C true CN1102148C (en) | 2003-02-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00102536A Expired - Lifetime CN1102148C (en) | 2000-03-03 | 2000-03-03 | Preparation method of scutellaria root extract |
Country Status (1)
| Country | Link |
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| CN (1) | CN1102148C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002333157A1 (en) * | 2002-09-03 | 2004-03-29 | Naturon Limited | Compound for treatment of anxiety and methods of preparation and use thereof |
| CN1325048C (en) * | 2005-11-09 | 2007-07-11 | 中国药科大学 | Application of wogonin for preparing medicine to treat or prevent hepatitis B |
| CN102366428B (en) * | 2007-08-07 | 2013-10-09 | 北京北大维信生物科技有限公司 | Applications of extracts from Chinese herbal medicine Oroxylum indicum in preparation of slimming and lipid lowering medicine or preparation of medicine with inhibition effect on activity of lipase |
| CN102357124B (en) * | 2011-10-25 | 2013-04-24 | 江苏济川制药有限公司 | Chinese medicine combination with effects of detumescence and inflammatory resistance and preparation method thereof |
| CN102397361A (en) * | 2011-11-26 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of granules for treating cold and cough |
| CN108853192A (en) * | 2018-08-09 | 2018-11-23 | 劲牌生物医药有限公司 | A kind of Baical Skullcap root P.E and preparation method thereof, application and health liquor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070189A (en) * | 1992-08-20 | 1993-03-24 | 佛山市制药二厂 | The production technique of flavonoid compound in the root of large-flowered skullcap |
-
2000
- 2000-03-03 CN CN00102536A patent/CN1102148C/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070189A (en) * | 1992-08-20 | 1993-03-24 | 佛山市制药二厂 | The production technique of flavonoid compound in the root of large-flowered skullcap |
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| Publication number | Publication date |
|---|---|
| CN1268512A (en) | 2000-10-04 |
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Granted publication date: 20030226 |