CN102397361A - Preparation method of granules for treating cold and cough - Google Patents
Preparation method of granules for treating cold and cough Download PDFInfo
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- CN102397361A CN102397361A CN201110381358XA CN201110381358A CN102397361A CN 102397361 A CN102397361 A CN 102397361A CN 201110381358X A CN201110381358X A CN 201110381358XA CN 201110381358 A CN201110381358 A CN 201110381358A CN 102397361 A CN102397361 A CN 102397361A
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Abstract
The invention relates to a preparation method of granules for treating cold and cough, which comprises the following steps that: kudzu root and radix scutellariae are taken and extracted by microwave and purified by polyamid chromatography columns to obtain kudzu root and radix scutellariae extract; radix bupleuri, Lonicera confusa DC., southernwood, forsythia, balloon flower and bitter almond are extracted under a carbon dioxide (CO2) supercritical condition to obtain the supercritical extract; and dextrine and sugar are added into the kudzu root and radix sutellariae extract as well as the supercritical extract after the kudzu root and radix sutellariae extract as well as the supercritical extract are mixed, then the kudzu root and radix sutellariae extract as well as the supercritical extract are made into granules and dried; and menthol is dissolved in ethanol to be sprayed into the granules, and the granules are uniformly mixed and are packed into 250g per bag. The granules for treating cold and cough, which are prepared through the method, are convenient to take.
Description
Technical field
The present invention relates to a kind of method for preparing of medicine, especially a kind of preparation method of Chinese medicine.
Background technology
The flu cough-stopping granule is by being processed as crude drug by Radix Bupleuri, Flos Lonicerae, Radix Puerariae, Herba Artemisiae Annuae, Fructus Forsythiae, Radix Scutellariae, Radix Platycodonis, Semen Armeniacae Amarum, Mentholum, recording in 2010 editions one one of the Pharmacopoeia of the People's Republic of China, the heat clearing away of inducing sweat, relieving cough and resolving phlegm.Be used for the flu due to the affection due to external wind and heat, disease sees that fever and aversion to wind, headache nasal obstruction, laryngopharynx swelling and pain, cough, the whole body are uncomfortable.
In the prior art, the report of Shang Weiyou flu cough-stopping granule aspect function of gallbladder promoting.
In the prior art, the flu cough-stopping granule prepare the method that adopts decocting to boil, technology is coarse, fall behind, impurity is many, causes patient's consumption per day excessive, is inconvenient to take, and has had a strong impact on these article and has used clinically.
Summary of the invention
Problem to be solved by this invention provides a kind of method for preparing of the cough-stopping granule of catching a cold, and makes it safe and effective, conveniently takes.
In order to solve the problems of the technologies described above, the present invention proposes following technical proposals.
Get Radix Puerariae 100g, Radix Scutellariae 100g, pulverize, mix, add 70% ethanol of 1.75L, drop in the microwave extracting apparatus and carry out microwave extracting; Extraction power 400-600W extracts 2 times, and each 5 minutes, combining extraction liquid; Concentrate, be added on the polyamide chromatography post, use 60% ethanol elution, collect 5 times of amount column volume eluents; Decompression recycling ethanol concentrates and drying, gets Pueraria lobota a kind of reed mentioned in ancient books extract, and is subsequent use; Get Radix Bupleuri 100g, Flos Lonicerae 75g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, pulverize, mix, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 2-4%, extracting pressure 15-30MPa, temperature 40-60 ℃, CO
2Flow 1-3ml/g crude drug min, extraction time 120-150min gets supercritical extract, and is subsequent use; Above-mentioned Pueraria lobota a kind of reed mentioned in ancient books extract, supercritical extract are mixed, add dextrin and sucrose, granulate, drying, 0.15g adds dissolve with ethanol with Mentholum, sprays in the granule, and mixing is processed 250g.
Radix Puerariae, Radix Scutellariae microwave extracting power 500W.
Polyamide is selected from a kind of in chinlon 66 (gathering adipoyl diamidogen), the chinlon 6 (polycaprolactam).
CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 3%.
CO
2The extracting pressure 20MPa of supercritical extraction, 50 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 130min.
Adopt the flu cough-stopping granule of technique scheme preparation, it is as the application of preparation function of gallbladder promoting medicine.
In the prior art; The flu cough-stopping granule need be taken 10g (being equivalent to the 6g medical material) at every turn; And the flu cough-stopping granule that adopts the present invention to be prepared from only need be taken 2.5g (being equivalent to the 6g medical material) at every turn, has significantly reduced dose having under the situation of similar pharmacologically active.This conclusion can be through following evidence.
Test the comparison of content of baicalin in the flu cough-stopping granule of distinct methods preparation.
1, instrument and reagent
Flu cough-stopping granule (supercritical methanol technology is hereinafter to be referred as G-LJ): press the preparation of embodiment 3 methods.
Flu cough-stopping granule (traditional method, be called for short G-CT), press 2010 editions methods preparations of the Pharmacopoeia of the People's Republic of China: i.e. Radix Bupleuri 100g, Flos Lonicerae 75g, Radix Puerariae 100g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Scutellariae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, Mentholum 0.15g, more than nine distinguish the flavor of; Except that Mentholum, eight flavors such as all the other Radix Bupleuri, decocte with water 2 times, each 4 hours; Decocting liquid filters, and filtrating merges, and is concentrated in right amount, adds sucrose and dextrin; Process granule, drying is after Mentholum adds an amount of dissolving of ethanol; Spray in the granule, mixing is processed 1000g.
Agilent 1200 high performance liquid chromatographs; METTLER AE240 electronic analytical balance.
Baicalin reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
2, method
Chromatographic condition Kromasi C18 chromatographic column (4.6mm * 200mm, 5 μ m); Methanol-water-phosphoric acid (50:50:0.2) is a mobile phase; Flow velocity 1.0mLmin
-1, detect wavelength 280nm.Precision is measured reference substance solution, each 10 μ L of need testing solution.
It is an amount of that the baicalin reference substance is got in the preparation of solution, and accurate the title decides, and adds methanol and processes the reference substance solution that every 1ml contains 60 μ g.Get offerings 1g respectively, the accurate title, decide, and puts in the tool plug conical flask, accurate 70% ethanol 25ml, the close plug of adding; Claim decide weight, supersound process (power 480W, frequency 40kHz) 30 minutes is put coldly, and weight decided in title again; Supply the weight that subtracts mistake with 70% ethanol, shake up, filter, precision is measured subsequent filtrate 10ml, puts in the 25ml measuring bottle; Add 70% ethanol to scale, shake up, filter, get subsequent filtrate, promptly get.
3, result
Result of the test is seen table 1, and the result shows that content of baicalin (115.1mg/ bag) in the flu cough-stopping granule that adopts the present invention's preparation is to adopt 4.2 times of traditional method (27.4 mg/ bag).
Content of baicalin in the flu cough-stopping granule of table 1 distinct methods preparation
| Sample | Content (mg/ bag) |
| G-LJ | 115.1 |
| G-CJ | 27.4 |
The flu cough-stopping granule pharmacodynamics of testing the preparation of two distinct methods compares.
1, test material.
1.1 animal
Kunming mouse; The SD rat.Provide by Shanghai Slac Experimental Animal Co., Ltd..
1.2 medicine
Flu cough-stopping granule (supercritical methanol technology is hereinafter to be referred as G-LJ): press the preparation of embodiment 3 methods.
Flu cough-stopping granule (traditional method, be called for short G-CT), press 2010 editions methods preparations of the Pharmacopoeia of the People's Republic of China: i.e. Radix Bupleuri 100g, Flos Lonicerae 75g, Radix Puerariae 100g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Scutellariae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, Mentholum 0.15g, more than nine distinguish the flavor of; Except that Mentholum, eight flavors such as all the other Radix Bupleuri, decocte with water 2 times, each 4 hours; Decocting liquid filters, and filtrating merges, and is concentrated in right amount, adds sucrose and dextrin; Process granule, drying is after Mentholum adds an amount of dissolving of ethanol; Spray in the granule, mixing is processed 1000g.
1.3 data and statistics
Data are represented with mean ± s, analyze with SPSS11.0 software.Note amount data is carried out the significance,statistical analysis with one factor analysis of variance, and enumeration data is used X 2 test.
2, method and result
2.1 influence to mouse cough
75 of mices, male and female half and half are divided 5 groups: the blank group at random; G-CT group (4.80g/Kg is equivalent to people's clinical dosage, i.e. every day 18g crude drug); The basic, normal, high dose groups of G-LJ (0.60g/Kg, 1.20g/Kg, 2.40g/Kg, wherein in dose groups be equivalent to people's clinical dosage, promptly every day the 18g crude drug).1h places in the inverted 500ml beaker after the mouse stomach administration, puts into the cotton balls of 0.3ml ammonia, observes and write down the number of times of coughing in mouse cough incubation period and 3 minutes immediately.
Result's (seeing table 2) shows, G-CT group, each dose groups of G-LJ all can significant prolongation ammonia cause mouse cough incubation period, reduce cough number of times (p < 0.05,0.01).Dosage is compared there was no significant difference among G-CT group, the G-LJ, is illustrated under the corresponding dosage (be equivalent to people's clinical dosage, i.e. every day 18g crude drug), and both antitussive actions are (p>0.05) quite.
The flu cough-stopping granule of table 2 distinct methods preparation is to the influence (n=15) of mouse cough
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
2.2 Oleum Tiglii is caused the influence of mice auricle swelling
Kunming mice, male and female half and half, body weight 18-22g is divided into 5 groups: the blank group at random; G-CT group (4.80g/Kg is equivalent to people's clinical dosage, i.e. every day 18g crude drug); The basic, normal, high dose groups of G-LJ (0.60g/Kg, 1.20g/Kg, 2.40g/Kg, wherein in dose groups be equivalent to people's clinical dosage, promptly every day the 18g crude drug).Gastric infusion.1h behind the medicine is coated with 2% Fructus Crotonis fluid, 50 μ l with mice left side ear, and 4h post-tensioning neck is put to death, and cuts two ears, sweeps away auricle with diameter 8mm rustless steel blunderbuss, weighs, and the difference of two auricle weight is the swelling degree, compares with matched group, calculates inhibitory rate of intumesce.
Result's (seeing table 3) shows, G-CT group, each dose groups of G-LJ all can significantly reduce the degree that Oleum Tiglii causes mice auricle swelling (p < 0.05,0.01), and G-CT group, the middle and high dose groups inhibitory rate of intumesce of G-LJ are all greater than 50%.Dosage is compared there was no significant difference among G-CT group, the G-LJ, is illustrated under the corresponding dosage (be equivalent to people's clinical dosage, i.e. every day 18g crude drug), and both antiphlogistic pharmacologically actives are (p>0.05) quite.
The flu cough-stopping granule of table 3 distinct methods preparation causes the influence (n=12) of mice auricle swelling to Oleum Tiglii
| Group | Dosage (g/Kg) | Auricle swelling degree (mg) | Suppression ratio (%) |
| Blank | - | 21.3±8.5 | ? |
| The G-CT group | 4.80 | 8.3±4.1** | 61.0 |
| The G-LJ low dosage | 0.60 | 12.6±5.5* | 40.8 |
| Dosage among the G-LJ | 1.20 | 8.2±5.4** | 61.5 |
| The G-LJ high dose | 2.40 | 7.5±4.2** | 64.8 |
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
2.3 influence to lumbar injection acetic acid mice capillary permeability
Kunming mice, male and female half and half, body weight 18-22g, grouping dosage and method are with 2.2.The 1 hour equal tail vein injection azovan blue of mice physiological salt liquid (0.2mg/0.1ml10g) behind the medicine; Lumbar injection 1% acetic acid 0.2ml/10g immediately; After half an hour mice is taken off neck and put to death,, survey absorbance with 721 type spectrophotometers (590nm) with quantitative normal saline flushing abdominal cavity; Make the azovan blue standard curve, draw the animal abdominal cavity thus and ooze out amount of dye.
Result's (seeing table 4) shows, G-CT group, each dose groups of G-LJ all can significantly reduce lumbar injection acetic acid mice capillary permeability (p < 0.05,0.01).Dosage is compared there was no significant difference among G-CT group, the G-LJ, is illustrated under the corresponding dosage (be equivalent to people's clinical dosage, i.e. every day 18g crude drug), and both antiphlogistic pharmacologically actives are (p>0.05) quite.
The flu cough-stopping granule of table 4 distinct methods preparation is to the influence (n=12) of lumbar injection acetic acid mice capillary permeability
| Group | Dosage (g/Kg) | Abdominal cavity sepage amount of dye (μ g/ only) |
| Blank | - | 6.33±1.32 |
| The G-CT group | 4.80 | 3.12±1.11** |
| The G-LJ low dosage | 0.60 | 3.94±1.21* |
| Dosage among the G-LJ | 1.20 | 3.14±1.15** |
| The G-LJ high dose | 2.40 | 3.02±0.97** |
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
2.4 the influence of Dichlorodiphenyl Acetate inducing mouse writhing response.
Get female kunming mice, body weight 18-22g, grouping, medication are with 2.2; 30min after the last administration, every Mus lumbar injection 0.6% glacial acetic acid 0.2mL, observe occur in the 15min turn round the body number of times; And calculating the writhing response suppression ratio, suppression ratio thinks that greater than 50% analgesic activity is arranged.
Result's (seeing table 5) shows, what each administration group all can significantly reduce the acetic-acid induced mice turns round the body number of times, compares p < 0.05,0.01 with the blank group.Wherein, the suppression ratio of G-CT group, the middle and high dose groups of G-LJ is all greater than 50%, and analgesic activity is obvious.Dose groups and G-CT group is compared unknown significance difference among the G-LJ, is illustrated under the corresponding dosage (all to be equivalent to people's clinical dosage) both biological activitys similar (p>0.05).
Table 5
The influence (n=12) of the flu cough-stopping granule Dichlorodiphenyl Acetate inducing mouse writhing response of distinct methods preparation
| Group | Dosage (g/Kg) | Turn round the body number of times (inferior/15min) | Suppression ratio (%) |
| Blank | - | 46.2±17.1 | —— |
| G-CT | 4.80 | 19.1±13.3** | 58.7 |
| The G-LJ low dosage | 0.60 | 26.4±13.5* | 42.9 |
| Dosage among the G-LJ | 1.20 | 19.0±12.8** | 58.9 |
| The G-LJ high dose | 2.40 | 15.3±11.1** | 66.9 |
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
2.5 bacteriostasis
Select staphylococcus aureus, alpha streptococcus, group B streptococcus, escherichia coli, Bacillus proteus.Bacteria culture media adopts MH meat soup.Medicine is made letter and is diluted than method, and medicine dilutes 5 examples with MH meat soup, every routine 10 pipes.Preceding 8 effective letters than dilution method with drug dilution.Back 2 pipes are cooked meat soup contrast respectively, the testing tube contrast, and testing tube adds above each bacterium 0.1mL respectively, puts 37 ℃ of incubators and cultivates observed result behind the 24h.
Result's (seeing table 6,7) shows; Adopt the flu cough-stopping granule of distinct methods preparation that experimental bacteria is all had stronger vitro inhibition effect; The MIC that wherein adopts the flu cough-stopping granule of traditional method preparation is 2 times of employing present inventor, shows that reducing dose can obtain similar pharmacologically active.
The external bacteriostasis of table 6 G-LJ
The external bacteriostasis of table 7 G-CT.
2.6 influence to rat fever due to the dry yeast
The SD rat, body weight 180-220g places experimental situation to adapt to 3-4 days, test preceding 2 days the set time with electric body-temperature instrumentation amount rat anus temperature 1 time, get the body temperature meansigma methods and supply to test use at 37.5-38.5 ℃ healthy rat.Rat is divided into 5 at random: the blank group; G-CT group (2.40g/Kg is equivalent to people's clinical dosage, i.e. every days 18 crude drug); The basic, normal, high dose groups of G-LJ (0.30g/Kg, 0.60g/Kg, 1.20g/Kg, wherein in dose groups be equivalent to people's clinical dosage, promptly every days 18 crude drug).Gastric infusion, continuous 4 days, fasting 12h before experiment in the 4th day; The same day was surveyed body temperature 3 times in experiment; Every at a distance from 1h1 time, behind the filling stomach relative medicine 30min, every rat back subcutaneous injection 15% dry yeast suspension 10mL/kg; Survey body temperature 1 time respectively at 3 after the administration, 4,5,10h, write down and obtain each and measure the body temperature changing value of the moment with respect to normal body temperature.
Result of the test (seeing table 8) shows, G-CT group, each dose groups of G-LJ all can significantly reduce the anus temperature that yeast causes the fever model rat (p < 0.05,0.01), more than the lasting 10h of effect ability.Dosage is compared there was no significant difference among G-CT group, the G-LJ, is illustrated under the corresponding dosage (all to be equivalent to people's clinical dosage) both biological activitys similar (p>0.05).
The flu cough-stopping granule of table 8 distinct methods preparation causes the influence (n=8) of fever model rat anus temperature to yeast
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
2.7 to rat bile secretion and bilirubinic influence
Male SD rat, body weight 180-220 gram is divided into 5 groups: the blank group at random; G-CT group (2.40g/Kg is equivalent to people's clinical dosage, i.e. every days 18 crude drug); The basic, normal, high dose groups of G-LJ (0.30g/Kg, 0.60g/Kg, 1.20g/Kg, wherein in dose groups be equivalent to people's clinical dosage, promptly every days 18 crude drug).After the fasting 12 hours (freely drinking water), lumbar injection crow smooth (1g/kg) anesthesia, back of the body position is fixing; 3cm opens abdomen under xiphoid-process, separates common bile duct, the nearly duodenum end of ligation; On common bile duct, make the V-arrangement clip, after inserting the faint yellow bile of biliary drainage my humble opinion and flowing out, fixing drainage tube; Close the abdominal cavity, stablize after 0.5 hour that to collect 0.5 hour bile earlier be contrast before the medicine.Thereafter each group gives different pharmaceutical through duodenum respectively, collects behind the medicine bile flow in 30,60,120,180 minutes respectively.Each animal day part bile of collecting merges, and measures content of bilirubin.
Result's (seeing table 9) shows, compares with the blank group, and the basic, normal, high dose groups of G-LJ all can significantly promote the secretory volume (p < 0.05,0.01) of rat bile, increases content of bilirubin (p < 0.05,0.01).Show that the bilocid sheet has tangible choleretic effect.Dosage is compared among G-CT group and the G-LJ; Choleresis, content of bilirubin significant difference (p 0.05); Be illustrated under the corresponding dosage and (be equivalent to the clinical consumption of people; Be 18g crude drug/>day), adopt the pharmacologically active of flu cough-stopping granule aspect function of gallbladder promoting of the present invention's preparation to be superior to adopting the traditional method person of preparation.
The flu cough-stopping granule of table 9 distinct methods preparation is to rat bile secretion and bilirubinic influence (n=6)
Annotate: compare * p with the blank group<0.05, * * p<0.01; Compare with dosage among the G-LJ,
△p<0.05.
Above-mentioned research shows, adopts the flu cough-stopping granule of the present invention's preparation, and active constituent content is 4.3 times of the flu cough-stopping granule that is equipped with of traditional law system.Reduce at dose under 3/4 the situation, both are suitable at antiinflammatory, analgesia, pharmacologically active aspect antibacterial.And the former activity aspect function of gallbladder promoting obviously is superior to the latter.The former can use in preparation function of gallbladder promoting medicine.
For technical scheme of the present invention better is described, hereinafter will be done further statement to its specific embodiment, but the scope that the present invention requires to protect is not limited to following embodiment.
The specific embodiment
Embodiment 1
Get Radix Puerariae 100g, Radix Scutellariae 100g, pulverize, mix, add 70% ethanol of 1.75L, drop in the microwave extracting apparatus and carry out microwave extracting; Extraction power 400W extracts 2 times, and each 5 minutes, combining extraction liquid; Concentrate, be added on chinlon 66 (gathering adipoyl diamidogen) the polyamide chromatography post, use 60% ethanol elution, collect 5 times of amount column volume eluents; Decompression recycling ethanol concentrates and drying, gets Pueraria lobota a kind of reed mentioned in ancient books extract, and is subsequent use; Get Radix Bupleuri 100g, Flos Lonicerae 75g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, pulverize, mix, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 2%, extracting pressure 15MPa, 40 ℃ of temperature, CO
2Flow 1ml/g crude drug min, extraction time 120min gets supercritical extract, and is subsequent use; Above-mentioned Pueraria lobota a kind of reed mentioned in ancient books extract, supercritical extract are mixed, add dextrin and sucrose, granulate, drying, 0.15g adds dissolve with ethanol with Mentholum, sprays in the granule, and mixing is processed 250g
Through detecting content of baicalin 104.5mg/ bag in the finished product.
Table 10 is seen in the pharmacological action of finished product cough-relieving.
Table 9 is seen in the pharmacological action hypersensitive of the anti-nasal mucosa of finished product.
The influence (n=15) of table 10 pair mouse cough
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
Embodiment 2
Get Radix Puerariae 100g, Radix Scutellariae 100g, pulverize, mix, add 70% ethanol of 1.75L, drop in the microwave extracting apparatus and carry out microwave extracting; Extraction power 600W extracts 2 times, and each 5 minutes, combining extraction liquid; Concentrate, be added on chinlon 6 (polycaprolactam) the polyamide chromatography post, use 60% ethanol elution, collect 5 times of amount column volume eluents; Decompression recycling ethanol concentrates and drying, gets Pueraria lobota a kind of reed mentioned in ancient books extract, and is subsequent use; Get Radix Bupleuri 100g, Flos Lonicerae 75g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, pulverize, mix, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 2-4%, extracting pressure 15-30MPa, temperature 40-60 ℃, CO
2Flow 1-3ml/g crude drug min, extraction time 120-150min gets supercritical extract, and is subsequent use; Above-mentioned Pueraria lobota a kind of reed mentioned in ancient books extract, supercritical extract are mixed, add dextrin and sucrose, granulate, drying, 0.15g adds dissolve with ethanol with Mentholum, sprays in the granule, and mixing is processed 250g.
Through detecting content of baicalin 112.8mg/ bag in the finished product.
Table 11 is seen in the pharmacological action of finished product cough-relieving.
The influence (n=15) of table 11 pair mouse cough
Annotate: compare with the blank group, * p 0.05, * * p < 0.01.
Embodiment 3
Get Radix Puerariae 100g, Radix Scutellariae 100g, pulverize, mix, add 70% ethanol of 1.75L, drop in the microwave extracting apparatus and carry out microwave extracting; Extraction power 500W extracts 2 times, and each 5 minutes, combining extraction liquid; Concentrate, be added on chinlon 6 (polycaprolactam) the polyamide chromatography post, use 60% ethanol elution, collect 5 times of amount column volume eluents; Decompression recycling ethanol concentrates and drying, gets Pueraria lobota a kind of reed mentioned in ancient books extract, and is subsequent use; Get Radix Bupleuri 100g, Flos Lonicerae 75g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, pulverize, mix, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 3%, extracting pressure 20MPa, 50 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 130min gets supercritical extract, and is subsequent use; Above-mentioned Pueraria lobota a kind of reed mentioned in ancient books extract, supercritical extract are mixed, add dextrin and sucrose, granulate, drying, 0.15g adds dissolve with ethanol with Mentholum, sprays in the granule, and mixing is processed 250g.
Claims (5)
1. the method for preparing of the cough-stopping granule of catching a cold is processed as crude drug by Radix Bupleuri 100g, Flos Lonicerae 75g, Radix Puerariae 100g, Herba Artemisiae Annuae 75g, Fructus Forsythiae 75g, Radix Scutellariae 75g, Radix Platycodonis 50g, Semen Armeniacae Amarum 50g, Mentholum 0.15g, it is characterized in that described method is made up of the following step: get Radix Puerariae, Radix Scutellariae, pulverize; Mix, add 70% ethanol of 1.75L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W; Extract 2 times, each 5 minutes, combining extraction liquid concentrated; Be added on the polyamide chromatography post, use 60% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol; Concentrate and drying, get Pueraria lobota a kind of reed mentioned in ancient books extract, subsequent use; Get Radix Bupleuri, Flos Lonicerae, Herba Artemisiae Annuae, Fructus Forsythiae, Radix Platycodonis, Semen Armeniacae Amarum 6 flavor medical materials, pulverize, mix, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 2-4%, extracting pressure 15-30MPa, temperature 40-60 ℃, CO
2Flow 1-3ml/g crude drug min, extraction time 120-150min gets supercritical extract, and is subsequent use; Above-mentioned Pueraria lobota a kind of reed mentioned in ancient books extract, supercritical extract are mixed, add dextrin and sucrose, granulate, drying adds dissolve with ethanol with Mentholum, sprays in the granule, and mixing is processed 250g.
2. according to the method for preparing of the said a kind of cough-stopping granule of catching a cold of claim 1, it is characterized in that said Radix Puerariae, Radix Scutellariae microwave extracting power 500W.
3. according to the method for preparing of the said a kind of cough-stopping granule of catching a cold of claim 1, it is characterized in that said polyamide is selected from a kind of in chinlon 66, the chinlon 6.
4. according to the method for preparing of the said a kind of cough-stopping granule of catching a cold of claim 1, it is characterized in that said CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 3%.
5. according to the method for preparing of the said a kind of cough-stopping granule of catching a cold of claim 1, it is characterized in that said CO
2The extracting pressure 20MPa of supercritical extraction, 50 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 130min.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
| CN108324778A (en) * | 2018-04-23 | 2018-07-27 | 哈尔滨市康隆药业有限责任公司 | A kind of preparation method of Sugarless type flu cough-stopping granule |
| CN110028595A (en) * | 2019-04-19 | 2019-07-19 | 安徽工程大学 | A kind of Deproteinated method of pueraria polysaccharide |
| CN111249359A (en) * | 2020-02-21 | 2020-06-09 | 覃迅云 | Yaoshan antipyretic drink formula |
| CN113144025A (en) * | 2021-05-24 | 2021-07-23 | 彭素岚 | Traditional Chinese medicine formula for treating mild influenza and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397362A (en) * | 2011-11-27 | 2012-04-04 | 苏州派腾生物医药科技有限公司 | Preparation method of granules for treating cold and cough |
| CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
| CN108324778A (en) * | 2018-04-23 | 2018-07-27 | 哈尔滨市康隆药业有限责任公司 | A kind of preparation method of Sugarless type flu cough-stopping granule |
| CN108324778B (en) * | 2018-04-23 | 2020-10-02 | 哈尔滨市康隆药业有限责任公司 | Preparation method of sugar-free cold and cough relieving granules |
| CN110028595A (en) * | 2019-04-19 | 2019-07-19 | 安徽工程大学 | A kind of Deproteinated method of pueraria polysaccharide |
| CN111249359A (en) * | 2020-02-21 | 2020-06-09 | 覃迅云 | Yaoshan antipyretic drink formula |
| CN113144025A (en) * | 2021-05-24 | 2021-07-23 | 彭素岚 | Traditional Chinese medicine formula for treating mild influenza and preparation method and application thereof |
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