CN107163616A - A kind of method and its purification process for extracting walnut green husk pigment - Google Patents
A kind of method and its purification process for extracting walnut green husk pigment Download PDFInfo
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- CN107163616A CN107163616A CN201710360769.8A CN201710360769A CN107163616A CN 107163616 A CN107163616 A CN 107163616A CN 201710360769 A CN201710360769 A CN 201710360769A CN 107163616 A CN107163616 A CN 107163616A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The present invention relates to a kind of method and its purification process for extracting walnut green husk pigment, belong to walnut technical field of pigment extraction, there is provided the chlorophyll removed before a kind of extraction in green peel of walnut, improve pigment degree of purity, improve the method and its purification process of the extraction walnut green husk pigment of Color, the technical scheme used is follows the steps below operation, a, remove chlorophyll, Exocarpium Juglandis Immaturus are air-dried, it is broken into powder, cross 50 mesh sieve and green peel of walnut powder is made, then Extraction solvent is used as using absolute ethyl alcohol, green peel of walnut powder and absolute ethyl alcohol are mixed, and extracted in a water bath, extract solution is filtered, and be concentrated under reduced pressure；B, thick pigment extraction, using ethanol as Extraction solvent, by by going the green peel of walnut powder and ethanol of chlorophyll to mix, and extracted in ultrasonic water bath, extraction process is ceaselessly stirred, then suction filtration, centrifugation, supernatant concentration is taken, dries and constant weight, obtains thick pigment；The present invention is widely used in the extraction of walnut green husk pigment.
Description
Technical field
The present invention relates to a kind of method and its purification process for extracting walnut green husk pigment, belong to walnut pigment extractive technique
Field.
Background technology
Walnut (Juglans regia L.) also known as English walnut, Qiang peach, it is among the people have " long live son ", " Longevity ", " intelligent promoting fruit ",
The title of " Beauty Fruits ", is Juglandaceae walnut, is a kind of nutritive value and economic value all very high precious fruit trees, and flat
Peach, cashew nut, fibert simultaneously claim the world four big nut.The with a long history of walnut is cultivated by China, and history records record walnut is Zhang Qian by the Western Regions
Take back seed plantation, then wide-scale distribution to the north each province.Xi Rong courts of a feudal ruler etc. are according to report both domestic and external in recent years, and combine archaeology
Learn, the research of cytology and zymetology, it is proposed that the original place of production of walnut includes China, it is believed that more than 7000 years it, walnut is in
State oneself through there is growth distribution.It is walnut nutritious abundant, with kidney-nourishing, stomach invigorating and moistening lung and the effects such as cerebrum tonifying.《Compendium of Materia Medica》Note
Carry, " walnut kernel, it is sweet mild-natured, can not only moistening dryness and resolving phlegm, can be with invigorating qi and benefiting blood and warm lung ease constipation ".The not only sweet property of walnut kernel
It is flat, it can be used as medicine, green peel of walnut, Walnut Leaves, root, inner septum of fruit etc. have application in terms of China's traditional medicine.
Walnut cultivated area is constantly expanding in recent years, and the seed selection and popularization to improved seeds are constantly being strengthened, to low yield
The transformation of woods is also in the process of quickening, and walnut yield riseing year by year, it is contemplated that full boundary walnut yield in 2015 be up to 400,000 tons with
On.With the collecting season in walnut, substantial amounts of discarded object occurs in producing region --- green peel of walnut, these discarded objects are seen everywhere,
Environment is not only broken up, the also great waste of precocity.Research shows, multiple biological activities composition is contained in green peel of walnut, such as many
Phenol, flavones, anthraquinone, naphthoquinones, alkaloid, polysaccharide etc., green peel of walnut are used as hair dye ancient.Walnut is in China point
Cloth is relatively broad, there is plantation more the various regions.At present, existing many researchers are directed to finding the new purposes of green peel of walnut and carried
Take method.
But existing extracting method is the coarse extraction to rascal pigment, does not account for rascal pigment Determination of Chlorophyll pair
The influence of pigment, obtained extract component is more complicated, can influence later Color.Do not speak of to extract yet
It is further purified.
The content of the invention
To solve the technical problem that prior art is present, the invention provides the leaf removed before a kind of extraction in green peel of walnut
Green element, improves pigment degree of purity, improves the method and its purification process of the extraction walnut green husk pigment of Color.
To achieve the above object, the technical solution adopted in the present invention is a kind of method for extracting walnut green husk pigment, is pressed
Operated according to following steps,
A, remove chlorophyll, Exocarpium Juglandis Immaturus are air-dried, and are broken into powder, cross 50 mesh sieve and green peel of walnut powder is made, then with anhydrous second
Alcohol is mixed green peel of walnut powder and absolute ethyl alcohol as Extraction solvent, and is extracted in a water bath, extract solution filtering,
And be concentrated under reduced pressure, finally go out to survey absorbance in 645nm, the extraction effect of chlorophyll is evaluated with color and absorbance；
B, thick pigment extraction, using ethanol as Extraction solvent, by green peel of walnut powder and the ethanol mixing by removing chlorophyll, and
Extracted in ultrasonic water bath, extraction process is ceaselessly stirred, then suction filtration, centrifugation 15min（2000r/min）, take supernatant
Liquid is concentrated, and is dried and constant weight, is obtained thick pigment.
It is preferred that, according to absolute ethyl alcohol and green peel of walnut powder liquid ratio it is 60 in the step a:1（ml/g）In 30 DEG C of water
Half is extracted in bath 30 minutes, be then 40 according still further to absolute ethyl alcohol and green peel of walnut powder liquid ratio:1（ml/g）In 40 DEG C of water-baths
Extract 20 minutes.
It is preferred that, using 55% ethanol as Extraction solvent in the step b, according to liquid ratio 25:1（ml/g）, at 45 DEG C
1 20min is extracted in ultrasonic water bath, 1 30min is extracted in 50 DEG C of ultrasonic water baths, is extracted 1 time in 55 DEG C of ultrasonic water baths
30min, extracts 1 30min in 60 DEG C of ultrasonic water baths.
It is preferred that, the step b can be replaced with following steps, using 55% ethanol as Extraction solvent, according to liquid material
Than 25:1（ml/g）After mixing, cellulase is added（0.4mg cellulases/green peel of walnut powder g）It is 4.66 to adjust pH, at 40 DEG C
Lower enzymolysis 60min, suction filtration, filtrate centrifugation 15min（2000r/min）, supernatant concentration simultaneously dry constant weight, obtain brown pigment.
A kind of purification process for extracting walnut green husk pigment, the thick pigment extracted is dissolved with 50% ethanol solution, adjusted
It is 6.3 to save pH, first determines and calculates pigment content；With pretreated macroporous absorbent resin wet method dress post, with the color for mixing up pH
Plain solution control is at 2 times of weight resin, and coutroi velocity is 3ml/min, is then carried out with 70% ethanol solution with 3ml/min
Elution, eluent is concentrated into volume during loading, determines the content of its pigment.
It is preferred that, solubility control of the thick pigment in 50% ethanol solution to 25%.
It is preferred that, the pretreatment of the large aperture resin：24h is soaked with the ethanol of 5 times of weight resins 95%, then with 3 times
95% ethanol and distilled water alternating elution 3 times, until ethanol eluate adds water not muddy, then be washed to a large amount of distillations
Property is standby.
Compared with prior art, the present invention has following technique effect：The present invention is using the side that chlorophyll is removed before extracting
Method, so as to get pigment it is purer, improve Color.And extracting method is simple, extraction efficiency is improved, work is easily realized
Industry metaplasia is produced.
Embodiment
In order that technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Embodiment is closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only to solve
The present invention is released, is not intended to limit the present invention.
Embodiment one
A kind of method for extracting walnut green husk pigment, follows the steps below operation,
A, remove chlorophyll, Exocarpium Juglandis Immaturus are air-dried, and are broken into powder, cross 50 mesh sieve and green peel of walnut powder is made, then with anhydrous second
Alcohol is 60 with green peel of walnut powder liquid ratio as Extraction solvent, absolute ethyl alcohol:1（ml/g）Partly 30 points are extracted in 30 DEG C of water-baths
Clock, is then 40 according still further to absolute ethyl alcohol and green peel of walnut powder liquid ratio:1（ml/g）Extract 20 minutes, carry in 40 DEG C of water-baths
Take liquid to filter, and be concentrated under reduced pressure, finally go out to survey absorbance in 645nm, the extraction of chlorophyll is evaluated with color and absorbance
Effect；
B, thick pigment extraction, using 55% ethanol as Extraction solvent, according to liquid ratio 25:1（ml/g）, in 45 DEG C of ultrasonic water
1 20min is extracted in bath, 1 30min is extracted in 50 DEG C of ultrasonic water baths, 1 30min is extracted in 55 DEG C of ultrasonic water baths,
1 30min is extracted in 60 DEG C of ultrasonic water baths, extraction process is ceaselessly stirred, then suction filtration, centrifugation 15min（2000r/min）,
Supernatant concentration is taken, dries and constant weight, obtains thick pigment.
Then processing is passivated to obtained thick pigment, specifically, by the thick pigment extracted with 50% ethanol solution
Dissolving, solubility is controlled to 25%, and it is 6.3 to adjust pH, first determines and calculates pigment content；With pretreated macroporous absorption tree
Fat wet method dress post, is controlled at 2 times of weight resin, coutroi velocity is 3ml/min, then with 70% with the pigment solution for mixing up pH
Ethanol solution eluted with 3ml/min, eluent is concentrated into volume during loading, determines the content of its pigment.
Wherein, the pretreatment of large aperture resin：24h is soaked with the ethanol of 5 times of weight resins 95%, then with the 95% of 3 times
Ethanol and distilled water alternating elution 3 times, until ethanol eluate adds water not muddy, then be washed to a large amount of distillations neutral standby
With.
Chemical reaction flows are belonged to using this method, equipment is simple, it is also simple and easy to apply to operate, and easily realizes industrial metaplasia
Production, extracts thick pigment efficiency and can reach 26.3%.Pigment can be used for industry to dye, clothes dyeing, ink and hair dye etc..
Embodiment two
A kind of method for extracting walnut green husk pigment, follows the steps below operation,
A, remove chlorophyll, Exocarpium Juglandis Immaturus are air-dried, and are broken into powder, cross 50 mesh sieve and green peel of walnut powder is made, then with anhydrous second
Alcohol is 60 with green peel of walnut powder liquid ratio as Extraction solvent, absolute ethyl alcohol:1（ml/g）Partly 30 points are extracted in 30 DEG C of water-baths
Clock, is then 40 according still further to absolute ethyl alcohol and green peel of walnut powder liquid ratio:1（ml/g）Extract 20 minutes, carry in 40 DEG C of water-baths
Take liquid to filter, and be concentrated under reduced pressure, finally go out to survey absorbance in 645nm, the extraction of chlorophyll is evaluated with color and absorbance
Effect；
B, thick pigment extraction, using 55% ethanol as Extraction solvent, according to liquid ratio 25:1（ml/g）After mixing, add fine
The plain enzyme of dimension（0.4mg cellulases/green peel of walnut powder g）It is 4.66 to adjust pH, and 60min, suction filtration, filtrate centrifugation are digested at 40 DEG C
15min（2000r/min）, supernatant concentration simultaneously dry constant weight, obtain brown pigment.
Then processing is passivated to obtained brown pigment, specifically, by the thick pigment extracted with 50% ethanol solution
Dissolving, solubility is controlled to 25%, and it is 6.3 to adjust pH, first determines and calculates pigment content；With pretreated macroporous absorption tree
Fat wet method dress post, is controlled at 2 times of weight resin, coutroi velocity is 3ml/min, then with 70% with the pigment solution for mixing up pH
Ethanol solution eluted with 3ml/min, eluent is concentrated into volume during loading, determines the content of its pigment.
Wherein, the pretreatment of large aperture resin：24h is soaked with the ethanol of 5 times of weight resins 95%, then with the 95% of 3 times
Ethanol and distilled water alternating elution 3 times, until ethanol eluate adds water not muddy, then be washed to a large amount of distillations neutral standby
With.
Enzyme assisted extraction method is belonged to using this method, enzyme can decompose the cell membrane of destruction plant cell, can drop
Low mass transfer resistance, improves extraction efficiency, and method extracts simple, and mild condition is environment-protecting and non-poisonous.Extract the efficiency energy of thick pigment
Reach 25.76%.The pigment that enzymatic assistant extraction is obtained belongs to pure natural no added pigment, can be used for all trades and professions such as food.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., all should be wrapped within the scope of the present invention.
Claims (7)
1. a kind of method for extracting walnut green husk pigment, it is characterised in that：Follow the steps below operation,
A, remove chlorophyll, Exocarpium Juglandis Immaturus are air-dried, and are broken into powder, cross 50 mesh sieve and green peel of walnut powder is made, then with anhydrous second
Alcohol is mixed green peel of walnut powder and absolute ethyl alcohol as Extraction solvent, and is extracted in a water bath, extract solution filtering,
And be concentrated under reduced pressure, finally go out to survey absorbance in 645nm, the extraction effect of chlorophyll is evaluated with color and absorbance；
B, thick pigment extraction, using ethanol as Extraction solvent, by green peel of walnut powder and the ethanol mixing by removing chlorophyll, and
Extracted in ultrasonic water bath, extraction process is ceaselessly stirred, then suction filtration, centrifugation 15min（2000r/min）, take supernatant
Liquid is concentrated, and is dried and constant weight, is obtained thick pigment.
2. a kind of method for extracting walnut green husk pigment according to claim 1, it is characterised in that：Pressed in the step a
It is 60 according to absolute ethyl alcohol and green peel of walnut powder liquid ratio:1（ml/g）Half is extracted in 30 DEG C of water-baths 30 minutes, then according still further to nothing
Water-ethanol is 40 with green peel of walnut powder liquid ratio:1（ml/g）Extracted 20 minutes in 40 DEG C of water-baths.
3. a kind of method for extracting walnut green husk pigment according to claim 1, it is characterised in that：In the step b with
55% ethanol is as Extraction solvent, according to liquid ratio 25:1（ml/g）, 1 20min is extracted in 45 DEG C of ultrasonic water baths, 50
1 30min is extracted in DEG C ultrasonic water bath, 1 30min is extracted in 55 DEG C of ultrasonic water baths, is extracted 1 time in 60 DEG C of ultrasonic water baths
30min。
4. a kind of method for extracting walnut green husk pigment according to claim 1, it is characterised in that：The step b can use
Following steps are replaced, using 55% ethanol as Extraction solvent, according to liquid ratio 25:1（ml/g）After mixing, fiber is added
Plain enzyme（0.4mg cellulases/green peel of walnut powder g）It is 4.66 to adjust pH, and 60min, suction filtration, filtrate centrifugation are digested at 40 DEG C
15min（2000r/min）, supernatant concentration simultaneously dry constant weight, obtain brown pigment.
5. a kind of purification process of extraction walnut green husk pigment as described in claim 1-4, it is characterised in that：By what is extracted
Thick pigment is dissolved with 50% ethanol solution, and regulation pH is 6.3, first determines and calculates pigment content；Inhaled with pretreated macropore
Attached resin wet method dress post, is controlled at 2 times of weight resin, coutroi velocity is 3ml/min, Ran Houyong with the pigment solution for mixing up pH
70% ethanol solution is eluted with 3ml/min, and eluent is concentrated into volume during loading, determines the content of its pigment.
6. a kind of purification process of extraction walnut green husk pigment as described in claim 1-4 according to claim 5, its
It is characterised by：Solubility of the thick pigment in 50% ethanol solution is controlled to 25%.
7. a kind of purification process of extraction walnut green husk pigment as described in claim 1-4 according to claim 5, its
It is characterised by：The pretreatment of the large aperture resin：24h is soaked with the ethanol of 5 times of weight resins 95%, then with the 95% of 3 times
Ethanol and distilled water alternating elution 3 times, until ethanol eluate adds water not muddy, then be washed to a large amount of distillations neutral standby
With.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710360769.8A CN107163616A (en) | 2017-05-22 | 2017-05-22 | A kind of method and its purification process for extracting walnut green husk pigment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109646993A (en) * | 2019-01-30 | 2019-04-19 | 山东省林业科学研究院 | A kind of method and apparatus for the red degradation product extracting plant chlorophyll |
| CN113801493A (en) * | 2021-10-14 | 2021-12-17 | 锦柯草木染(上海)纺织科技有限公司 | Method for extracting natural dye from outer skin of pecan |
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| CN102161835A (en) * | 2011-03-11 | 2011-08-24 | 浙江农林大学 | Method for preparing pigment by waste pecan epicarp |
| CN103333519A (en) * | 2013-07-18 | 2013-10-02 | 黑龙江生态工程职业学院 | Hickory nut shell brown pigment and preparation method thereof |
| CN104910684A (en) * | 2015-06-08 | 2015-09-16 | 昆明理工大学 | Walnut green husk pigment ink and preparation method thereof |
| CN105361185A (en) * | 2015-11-12 | 2016-03-02 | 横山县红梦园科技实业有限公司 | Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin |
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2017
- 2017-05-22 CN CN201710360769.8A patent/CN107163616A/en active Pending
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|---|---|---|---|---|
| CN102161835A (en) * | 2011-03-11 | 2011-08-24 | 浙江农林大学 | Method for preparing pigment by waste pecan epicarp |
| CN103333519A (en) * | 2013-07-18 | 2013-10-02 | 黑龙江生态工程职业学院 | Hickory nut shell brown pigment and preparation method thereof |
| CN104910684A (en) * | 2015-06-08 | 2015-09-16 | 昆明理工大学 | Walnut green husk pigment ink and preparation method thereof |
| CN105361185A (en) * | 2015-11-12 | 2016-03-02 | 横山县红梦园科技实业有限公司 | Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109646993A (en) * | 2019-01-30 | 2019-04-19 | 山东省林业科学研究院 | A kind of method and apparatus for the red degradation product extracting plant chlorophyll |
| CN109646993B (en) * | 2019-01-30 | 2020-12-22 | 山东省林业科学研究院 | Method and device for extracting red degradation product of plant chlorophyll |
| CN113801493A (en) * | 2021-10-14 | 2021-12-17 | 锦柯草木染(上海)纺织科技有限公司 | Method for extracting natural dye from outer skin of pecan |
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