Background technology
The method of existing manufacturing collagen polypeptide is that to adopt animal skin be raw material, and its technical process mostly is raw material washing decontamination → cut apart non-collagenous tissue → washing → strong acid or highly basic pre-treatment → disacidify or lixiviating → washing → heating (about 100 ℃) pre-treatment → substep enzymolysis → separation and purification → sterilization → packing or spraying drying → product that skin becomes fritter → washing → removal grease → washing → removal intracutaneous.A large amount of chemical reagent that use in the existing processes flow process, as washing composition, trichogen, grease-removing agent etc., and used chemical reagent easy-clear is unclean.The non-collagen component of used raw material intracutaneous is difficult to remove as the dirt in fat, the pore, will influence purity, taste and the quality of collagen polypeptide products.If adopt the animal tendon to make raw material, though the production cycle is shorter, it is difficult to handle oil film, sarcolemma, and raw material is limited, not easily collecting.Existing process using microbial enzyme, as the Asl.398 enzyme, Sumizyme MP, though have very strong hydrolysis ability, because of used enzyme preparation is difficult to purify, the peculiar smell of zymin own is heavy, the hydrolysate taste is bitter.By the collagen polypeptide of existing explained hereafter exist the production cycle long, technology is loaded down with trivial details, product ubiquity bitter taste, the defective that peculiar smell is too heavy.In addition, water consumption is big in the production, and the energy consumption height pollutes big.Collagen polypeptide molecular weight ranges with existing explained hereafter is big.
Summary of the invention
The technical problem to be solved in the present invention provide a kind of with short production cycle, technology is simple, product does not have the manufacturing collagen polypeptide technology of bitter taste, peculiar smell, the molecular weight of the collagen polypeptide of being produced is adjustable in certain limit.
The technical solution adopted for the present invention to solve the technical problems is: a kind of manufacture method of collagen polypeptide is a raw material with the gelatin, by with the gelatin dissolving, under 45-65 ℃ temperature, glue is adjusted to slightly acidic, adds biological enzyme raw material is degraded to collagen polypeptide.
According to the collagen polypeptide that the manufacture method of above collagen polypeptide is produced, molecular weight is 1000-5000; 3000-5000; 5000-8000.Reach 99% in over dry thing total amino acid content; Ash (Ash)<0.5%, heavy metal content (plumbous, arsenic)≤0.25mg/kg; There are not obvious bitter taste, peculiar smell; Solid-state is white powder, soluble in water.
The invention has the beneficial effects as follows: with short production cycle, technology is simple, product does not have bitter taste, peculiar smell, and the molecular weight of the collagen polypeptide of being produced is more concentrated.Simultaneously, water consumption is less in the production, and energy consumption is low, pollutes little.
Specific embodiments
Get 10 parts in gelatin, add water 30-200 part, at room temperature swelling is no more than 5 hours, and heated and stirred is fully dissolved it, under 45-65 ℃ temperature, with suitable alkali or acid the pH value of glue is adjusted to 5.0-7.0, adds biological enzyme 0.01-3.0 part, enzymolysis was not less than 4 hours, intensification is not less than 80 ℃, insulation was not less than 20 minutes, and cooling is not less than 40 ℃, promptly got liquid collagen polypeptide through separation, purifying; Or, promptly get the solid state collagen polypeptide with liquid collagen polypeptide drying.
Embodiment 1:
Get 10 parts in gelatin, add 30 parts of pure water, swelling is 0.5 hour under the room temperature, heated and stirred to dissolving evenly, with ammoniacal liquor or NaOH solution the pH value of glue is adjusted to 7.0, be warming up to 45 ℃, add 0.01 part of pancreatin, Alcalase proteinase-10 .005-0.2 part, enzymolysis is no less than 1 hour; Add 0.01 part of food flavor enzyme, enzymolysis 1 hour adds 0.01 part of bromeline again, and enzymolysis 2 hours is warming up to 80 ℃, is incubated 15 minutes, is cooled to 40 ℃.Through separation and purification, ultraviolet disinfection, liquid subpackage or spraying drying, aseptic subpackaged, promptly get collagen polypeptide products.
Embodiment 2:
Get 10 parts in gelatin, add 100 parts of pure water, swelling is 3 hours under the room temperature, heated and stirred to dissolving evenly, with formic acid the pH value of glue is adjusted to 5.0, be warming up to 50 ℃, add 0.05 part of pancreatin, 0.01 part of food flavor enzyme, enzymolysis 6 hours; Add 0.01 part of bromeline again, enzymolysis 3 hours is warming up to 90 ℃, is incubated 20 minutes, is cooled to 45 ℃.Through separation and purification, ultraviolet disinfection, liquid subpackage or spraying drying, aseptic subpackaged, promptly get collagen polypeptide products.
Embodiment 3:
Get 10 parts in gelatin, add 130 parts of pure water, swelling is 4 hours under the room temperature, and heated and stirred is even to dissolving, with KOH solution the pH value of glue is adjusted to 7.0, is warming up to 55 ℃, add 1 part of pancreatin, add 0.5 part of food flavor enzyme, enzymolysis 24 hours is warming up to 90 ℃, is incubated 18 minutes, is cooled to 45 ℃.Through separation and purification, ultraviolet disinfection, liquid subpackage or spraying drying, aseptic subpackaged, promptly get collagen polypeptide products.
Embodiment 4:
Get 10 parts in gelatin, add 200 parts of pure water, swelling is 5 hours under the room temperature, and heated and stirred is even to dissolving, with HCL solution the pH value of glue is adjusted to 6.5, is warming up to 45 ℃, add 1.2 parts of pancreatin, enzymolysis 20 hours, be warming up to 85 ℃, be incubated 20 minutes, be cooled to 43 ℃.Through separation and purification, ultraviolet disinfection, liquid subpackage or spraying drying, aseptic subpackaged, promptly get collagen polypeptide products.
Embodiment 5:
Get 10 parts in gelatin, add 180 parts of pure water, swelling is 3 hours under the room temperature, heated and stirred is even to dissolving, with ammoniacal liquor or NaOH solution the pH value of glue is adjusted to 6.8, is warming up to 48 ℃, add 0.3 part of pancreatin, add 0.06 part of food flavor enzyme, add 0.1 part of bromeline, enzymolysis 12 hours is warming up to 85 ℃, be incubated 19 minutes, be cooled to 45 ℃.Through separation and purification, ultraviolet disinfection, liquid subpackage or spraying drying, aseptic subpackaged, promptly get collagen polypeptide products.
Embodiment 6:
Get 10 parts in gelatin, add 150 parts of pure water, swelling is 3.0 hours under the room temperature, and heating for dissolving is even, regulate pH value 7.0, be warming up to 47 ℃, add 0.01 part of pancreatin, Alcalase proteinase-10 .02 part, 0.001 part of 0.1 part of food flavor enzyme and bromeline, enzymolysis is no less than 4 hours.Be warming up to 90 ℃, be incubated 15 minutes, be cooled to 45 ℃.Through separation and purification, ultraviolet disinfection or spraying drying, the aseptic subpackaged collagen polypeptide products that promptly gets.
By top embodiment the present invention is specifically described, what will spell out at this is that above embodiment only is used for the present invention is further specified, and can not be interpreted as limiting the scope of the invention.This art skilled person can make some nonessential improvement and adjustment to the present invention according to the foregoing invention content.