CN1395829A - Antibacterial nano silver powder using algae as carrier and its preparing process - Google Patents
Antibacterial nano silver powder using algae as carrier and its preparing process Download PDFInfo
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- CN1395829A CN1395829A CN 02138891 CN02138891A CN1395829A CN 1395829 A CN1395829 A CN 1395829A CN 02138891 CN02138891 CN 02138891 CN 02138891 A CN02138891 A CN 02138891A CN 1395829 A CN1395829 A CN 1395829A
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Abstract
An antibacterial silver nanometre particles using algae as carrier and its preparing process are disclosd. The silver nanometre particles (1-100 nm) are firmly adsorbed in the cavities or cells of algae. Its advantages are wide application range, and long-affective broad-spectrum bactericidal power.
Description
Technical field
The present invention relates to a kind of nano-silver antimicrobial powder and preparation method thereof, particularly relating to a kind of is the nano-silver antimicrobial powder and preparation method thereof of carrier with the algae.
Background technology
In recent years, the surfaces externally and internally by porous material is that main body is carried out the self assembly of nanoparticle and the research focus that the load synthetic technology has become field of nanometer material technology.
According to data: silver type antibacterial agent has the performance better than organic antibacterial agent aspect safety, persistence, broad-spectrum antibacterial property, the warm tolerance.So this class material just has been applied to life and medical aspect in the middle of the 19th century.From twentieth century eighties, abroad have numerous scholars to the nanometer silver type antibacterial agent assembling preparation in different materials study, especially Japan's research has in this respect obtained bigger progress, and the part achievement has realized industrialization.Today, the research of China's nanometer silver type antibacterial agent has also obtained carrying out energetically, and related industry also begins flourish.Chinese invention patent 01110767 " aggregation-preventing nanometer wide-spectrum antibacterial silve powder and inductrial production process " discloses a kind of aggregation-preventing nanometer wide-spectrum antibacterial silve powder and inductrial production process, with nano silver powder securely attached on some natural porous crack vegetable materials, and do not consider to adopt algae to adsorb micro silver powder, make that Nano Silver type antimicrobial product is on the high side.Besides causing water, pollutes algae proliferation, it is one of the most serious problem of being faced of mankind nowadays and other life forms that water pollutes, and the water quality eutrophication is the hang-up that puzzlement most countries improvement water pollutes, this all is to cause because of algae, these algae mainly contain spirulina, microcytis, Anabaena etc., their common feature is that inside exists pore space structure, and porose bubble in the cell utilizes this design feature of algae to come assemble nanometer silvery to be equipped with the nanometer silver type antibacterial agent.
Summary of the invention
The objective of the invention is to pollute in order to administer serious day by day water, and reduce the production cost of nanometer silver type antibacterial agent, is the nano-silver antimicrobial powder and preparation method thereof of carrier with the algae and provide a kind of.
It is the nano-silver antimicrobial powder of carrier with the algae that the present invention is achieved in that a kind of, is firmly adsorbing nano-Ag particles in the cavity of algae or ghost, and its particle diameter is the 1-100 nanometer.Described algae is blue-green algae, green alga or diatom, and it is anabena, spirulina, arthrospira, chamber ball algae or joint ball algae that blue-green algae has microcytis, Anabaena.
The preparation method who with the algae is the nano-silver antimicrobial powder of carrier has two kinds:
(1) preparation AgNO
3Solution, algae immersion, separation cleaning, oven dry, grinding dispersion, illumination, cleaning, oven dry make product, and wherein separation cleaning employing centrifugation is cleaned, and wash number is 2 times, and light application time is 0.5-1 hour.
(2) preparation AgNO
3Solution, algae are soaked, separate and clean, soak half an hour in reductant solution, separate and washing, and oven dry makes product again, and wherein reductant can be selected formaldehyde or glucose solution etc. for use.
AgNO
3The concentration of solution is 0.001～0.010molL
-1
The nano-silver antimicrobial powder that the present invention is prepared adds suitable medicinal adjuvant or excipients can make pulvis, tablet, paste, creme, film, gel, patch, injection, aqua, spray or biological medicine chip; The nano silver antimicrobials of prepared with the algae is carrier can be used as broad spectrum antimicrobicide.
" development of inorganic antiseptic and application " material Leader of Huang Zhanjie, 1999,13 (2): 35 and " development of silver-colored type inorganic antiseptic and application thereof " material Leader of Feng Naiqian, Yan Jianhua, 1998,12 (2): the sterilization mechanism of introducing nano silver antimicrobials in 1:
The one, the haptoreaction of silver ion promptly after silver ion in the antibacterial product and the bacterium haptoreaction, causes the microorganism proper constituent to destroy or produce functional disorder.When the silver ion of trace arrives the microbial cell film, electronegative because of the latter, rely on Coulomb attraction, both are firmly adsorbed,
Silver ion penetration cell wall enters in the cell, and (SH) reaction makes protein coagulating, destroys the activity of cell synzyme, and cell is lost the division growth ability and death with base.
Silver ion can also destroy microorganisms electric transmission system, respiratory system and substance delivery system.After thalline lost activity, silver ion can dissociate out from thalline again, repeats fungicidal property, so its antibacterial effect is lasting.
The 2nd, light-catalyzed reaction, the trace silver of matter surface distributed under the effect of light, can play the effect of catalytic active center, activates the oxygen in the water and air, produces hydroxyl radical free radical (OH
-) and active oxygen ion (O
-2), active oxygen ion has very strong oxidability, can destroy the multiplication capacity of bacterium at short notice and makes cell death, thereby reach antibiotic purpose.
Product of the present invention can be used as the medical material of treatment burn and scald, material with the skin infection for the treatment of traumatic patient and skin and prevention of surgical incision postoperative and treatment infection of incisional wound, also can be widely used in fields such as enamel, chemical fibre, commodity, plastic articles, tame electrical article, coating, paint, water treatment, sports goods, medical and hygiene article, cosmetics, building building, have broad spectrum antibacterial, long-lasting, kill excellent properties such as drug resistance.From raw material sources, production technology, overcome the general higher shortcoming of numerous silver-colored type antimicrobial product prices on the current market, this not only greatly reduces the production cost of silver type antibacterial agent, and helps administering serious day by day water pollution problems, has very big environment protection significance.Therefore, the present invention not only has very high economic benefit, and can obtain the huge social benefit.
Embodiment
At this is that example elaborates preparation process with the spirulina.
Embodiment 1:
(1), takes by weighing 0.05g silver nitrate AgNO
3, be dissolved in 80ml distilled water, obtaining concentration is 0.004molL
-1AgNO
3Solution;
(2), take by weighing the spirulina powder of 2.0g drying, put into AgNO
3Soak in the solution, till agitation as appropriate makes spirulina powder soak thoroughly;
(3), centrifugation AgNO
3Solution and spirulina powder are poured out upper strata AgNO
3Behind the solution, add an amount of distilled water, centrifugation more immediately after shaking up is according to said method cleaned once with distilled water then again, and it is fast that cleaning process is wanted, and avoids the AgNO that adsorbs in the spiral frond at once
3Come out;
(4), above-mentioned separating obtained sample is dried (can adopt atomized drying in batches) under about 80 ℃ of conditions;
(5), dried sample is suitably ground dispersion;
(6), with sample with placements of making thinner of bigger vessel, and, in the illumination process, suitably stir sample and make illumination even with solar radiation sample 1 hour, illumination has just formed the nano-Ag particles that is evenly distributed in the spiral frond afterwards;
(7), clean sample, wash residual AgNO with distilled water
3Deng;
(8), oven dry sample.
Transmission electron microscope observing result to sample shows: nano-Ag particles is uniformly distributed in the spiral frond, and the nano-Ag particles size distribution is in 10～50nm, the about 30nm of particle mean size.Promptly obtained with the spirulina is the nano-silver antimicrobial powder of carrier.
Embodiment 2:
(1), takes by weighing 0.05g silver nitrate AgNO
3, be dissolved in 80ml distilled water, obtaining concentration is 0.004molL
-1AgNO
3Solution;
(2) take by weighing the spirulina powder of 2.0g drying, put into AgNO
3Soak in the solution, till agitation as appropriate makes spirulina powder soak thoroughly;
(3), separate AgNO
3Solution and spirulina powder are poured out upper strata AgNO
3Behind the solution, add an amount of distilled water, centrifugation more immediately after shaking up is according to said method cleaned once with distilled water then again, and it is fast that cleaning process is wanted, and avoids the AgNO that adsorbs in the spiral frond at once
3Come out;
(4), add an amount of formaldehyde and soak sample, soak time is half an hour approximately, formaldehyde makes the AgNO of absorption in the spiral frond
3In Ag
+Reduce and the formation nano-Ag particles;
(5), adopt centrifugation and clean sample again with distilled water;
(6), oven dry sample.
Transmission electron microscope observing result to sample shows: nano-Ag particles is uniformly distributed in the spiral frond, and the nano-Ag particles size distribution is in 10～70nm, the about 40nm of particle mean size.Promptly obtained with the spirulina is the nano-silver antimicrobial powder of carrier.
Claims (10)
1, a kind of is the nano-silver antimicrobial powder of carrier with the algae, it is characterized in that firmly adsorbing nano-Ag particles in the cavity of algae or ghost, and its particle diameter is the 1-100 nanometer.
2, nano-silver antimicrobial powder according to claim 1 is characterized in that described algae is blue-green algae, green alga or diatom.
3, nano-silver antimicrobial powder according to claim 2 is characterized in that described blue-green algae is that microcytis, Anabaena are anabena, spirulina, arthrospira, chamber ball algae or joint ball algae.
4, the preparation method of nano-silver antimicrobial powder according to claim 1 is characterized in that comprising preparation AgNO
3Solution, the immersion of algae powder, separation cleaning, oven dry, grinding dispersion, illumination, cleaning, oven dry make product.
5, the preparation method of nano-silver antimicrobial powder according to claim 1 is characterized in that comprising preparation AgNO
3Solution, algae powder soak, separate and clean, soak half an hour in reductant solution, separate and washing, and oven dry makes product again.
6, the preparation method of nano-silver antimicrobial powder according to claim 5 is characterized in that reductant is formaldehyde or glucose solution.
7, according to the preparation method of claim 4 or 5 described nano-silver antimicrobial powders, it is characterized in that AgNO
3The concentration of solution is 0.001-0.010molL
-1
8, according to the preparation method of claim 4 or 5 described nano-silver antimicrobial powders, it is characterized in that described separation cleaning employing centrifugation cleaning, wash number is 2 times.
9, the preparation method of nano-silver antimicrobial powder according to claim 4 is characterized in that light application time is 0.5-1.0 hour.
10, nano-silver antimicrobial powder according to claim 1 is characterized in that prepared nano-silver antimicrobial powder is added suitable medicinal adjuvant or excipients can make pulvis, tablet, paste, creme, film, gel, patch, injection, aqua, spray or biological medicine chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02138891 CN1243471C (en) | 2002-08-06 | 2002-08-06 | Antibacterial nano silver powder using algae as carrier and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02138891 CN1243471C (en) | 2002-08-06 | 2002-08-06 | Antibacterial nano silver powder using algae as carrier and its preparing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1395829A true CN1395829A (en) | 2003-02-12 |
| CN1243471C CN1243471C (en) | 2006-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02138891 Expired - Fee Related CN1243471C (en) | 2002-08-06 | 2002-08-06 | Antibacterial nano silver powder using algae as carrier and its preparing process |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005120173A3 (en) * | 2004-05-12 | 2006-04-27 | Kishore Madhukar Paknikar | Anti-microbial activity of biologically stabilized silver nano particles |
| CN104525965A (en) * | 2014-12-25 | 2015-04-22 | 广东省微生物研究所 | Method for preparing nano-silver by means of microalgae |
| CN106609477A (en) * | 2016-11-14 | 2017-05-03 | 蚌埠市风驰滤清器有限公司 | An oil-proof filter paper for filters |
| CN107597069A (en) * | 2017-10-20 | 2018-01-19 | 烟台宇诚企业管理咨询有限公司 | It is a kind of degerming except the sorbing material of formaldehyde containing marine organism extract |
-
2002
- 2002-08-06 CN CN 02138891 patent/CN1243471C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005120173A3 (en) * | 2004-05-12 | 2006-04-27 | Kishore Madhukar Paknikar | Anti-microbial activity of biologically stabilized silver nano particles |
| EA013401B1 (en) * | 2004-05-12 | 2010-04-30 | Нано Каттинг Эдж Текнолоджиз Пвт. Лтд. | Antimicrobial activity of biologically stabilized silver nano particles |
| CN104525965A (en) * | 2014-12-25 | 2015-04-22 | 广东省微生物研究所 | Method for preparing nano-silver by means of microalgae |
| CN104525965B (en) * | 2014-12-25 | 2016-06-29 | 广东省微生物研究所 | A kind of method utilizing microalgae to prepare nanometer silver |
| CN106609477A (en) * | 2016-11-14 | 2017-05-03 | 蚌埠市风驰滤清器有限公司 | An oil-proof filter paper for filters |
| CN107597069A (en) * | 2017-10-20 | 2018-01-19 | 烟台宇诚企业管理咨询有限公司 | It is a kind of degerming except the sorbing material of formaldehyde containing marine organism extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1243471C (en) | 2006-03-01 |
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