CN104525965A - Method for preparing nano-silver by means of microalgae - Google Patents
Method for preparing nano-silver by means of microalgae Download PDFInfo
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- CN104525965A CN104525965A CN201410830502.7A CN201410830502A CN104525965A CN 104525965 A CN104525965 A CN 104525965A CN 201410830502 A CN201410830502 A CN 201410830502A CN 104525965 A CN104525965 A CN 104525965A
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Abstract
The invention discloses a simple method for preparing nano-silver by means of microalgae. According to the simple method for preparing nano-silver by means of microalgae, a solution of chlorella or limnothrix or scenedesmus quaclricauda is centrifuged, algal cells are deposited and re-suspended in sterilized deionized water, ice-bathing and breaking are conducted by placing the sterilized deionized water containing the algal cells under ultrasonic waves, breaking is conducted for three to ten minutes under the condition that the output power ranges from 150 W to 200 W, centrifugation is conducted, algal sediment is removed, silver nitrate is added to the algal solution, the concentration of silver ions ranges from 5 microgram/mL to 100 microgram/mL, the oscillation reaction is conducted for one to five hours by placing the mixture on a water bath shaker at the temperature of 20-40 DEG C, and then a nano-silver solution is obtained by conducting ultrasonic treatment and membrane filtering. According to the method, nano-silver particles are prepared by means of microalgae, the preparation method is simple, easy to implement, high in operability and low in cost, no toxic and harmful substance is discharged into the environment during preparation, and the method is a simple and environmental-friendly technique for preparing nano-silver.
Description
Technical field:
The invention belongs to biological technical field, be specifically related to a kind of method utilizing micro-algae to prepare Nano Silver.
Background technology:
As far back as the ancient times, argent is just recognized to have antibacterial and bactericidal action by people, and has the antibacterial activity of wide spectrum.In recent years, to the New research progress of metal nanoparticle, promoted the application of Nano Silver in antibacterial field, research finds all have efficient antibacterial activity with Nano Silver prepared by multiple method.Nano Silver is called as the antiseptic of 21st century a new generation, and it has wide spectrum, efficiently anti-microbial property, and Nano Silver was widely used already as anti-biotic material, as being used in burn dressing, operating table, water purification installation and some Medical Devices etc.Based on the anti-microbial property that Nano Silver is excellent, it has a extensive future, and has the market demand widely in consumer goods industries, medicine and hygiene fields, building materials field, electronics industry etc.
The preparation method of nano silver particles is a lot, the mainly chemical method such as solution phase chemical reduction, electrochemical process, photochemical reduction, with physical method such as reduction ball-milling method, using vaporization condensation process, atomization etc., wherein especially more common with chemical reduction method, but also need strictly to control reaction condition, higher to the requirement of technology of preparing.
Summary of the invention:
The object of this invention is to provide a kind of simple and easy to do, workable, with low cost, and preparation process can not discharge poisonous and harmful substance to environment, micro-algae that utilizes of comparing environmental protection prepares the method for Nano Silver.
The present invention utilizes micro-algae to prepare the method for Nano Silver, it is characterized in that, by centrifugal to chlorella, silk algae or Scenedesmus quadricauda algae liquid, frustule precipitation is resuspended in the water of sterilizing, under being placed in ultrasonic wave, ice bath is broken, centrifugal, remove algae precipitation, in the algae liquid of supernatant, add silver nitrate, the final concentration of silver ion is 5-100 μ g/mL, be placed in 20-40 DEG C of water-bath concussion reaction 1-5h again, then through ultrasonic process, membrane filtration, filtrate is Nano silver solution.
Preferably, described in be placed in the broken and ultrasonic process of ice bath under ultrasonic wave, its ultrasonic power output is 150-200W, broken 3-10min.
Described filtration is preferably with the membrane filtration of aperture 0.1-0.22 μm.
The present invention utilizes micro-algae to prepare nano silver particles, and this preparation method is simple and easy to do, workable, with low cost, and preparation process can not discharge poisonous and harmful substance to environment, is a not only simple but also nano silver preparation technology for environmental protection.
Accompanying drawing illustrates:
Fig. 1-Fig. 6 is the transmission electron microscope picture of the Nano silver solution of embodiment 1-6 respectively.
Detailed description of the invention:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
Cultured chlorella algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, micro-for weight in wet base 1g algae is resuspended in 20mL deionized water, and under condition of ice bath ultrasonication frustule, ultrasonic power output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the final concentration of silver ion be 10 μ g/mL, be placed in 30 DEG C of oscillating reactions 5h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.By the membrane filtration of the solution after ultrasonic with 0.22 μm, obtain the Nano silver solution clarified.As shown in Figure 1, as can be seen from Figure 1, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Embodiment 2:
Cultured chlorella algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, weight in wet base 2g frustule precipitation is resuspended in the deionized water of 20ml sterilizing, and under condition of ice bath ultrasonication frustule, power output is 200 watts, ultrasonic 1min, interval 1min, repeats 10 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the final concentration of silver ion be 20 μ g/mL, be placed in 40 DEG C of oscillating reactions 5h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 3 times.By the membrane filtration of the solution after ultrasonic with 0.22 μm, obtain the Nano silver solution clarified.As shown in Figure 2, as can be seen from Figure 2, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Embodiment 3:
Cultured chlorella algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, weight in wet base 5g frustule precipitation is resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, power output is 200 watts, ultrasonic 1min, interval 1min, repeats 5 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the concentration of silver ion be 50 μ g/mL, be placed in 20 DEG C of oscillating reactions 5h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 10 times.By the membrane filtration of the solution after ultrasonic with 0.22 μm, obtain the Nano silver solution clarified.As shown in Figure 3, as can be seen from Figure 3, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Embodiment 4:
Cultured chlorella algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, weight in wet base 5g frustule precipitation is resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, power output is 200 watts, ultrasonic 1min, interval 1min, repeats 3 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the concentration of silver ion be 100 μ g/mL, be placed in 30 DEG C of oscillating reactions 5h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.By the membrane filtration of the solution after ultrasonic with 0.22 μm, obtain the Nano silver solution clarified.As shown in Figure 4, as can be seen from Figure 4, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Embodiment 5:
Cultured Scenedesmus quadricauda algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, weight in wet base 1g frustule precipitation is resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the final concentration of silver ion be 10 μ g/mL, be placed in 20 DEG C of oscillating reactions 5h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.By the membrane filtration of the solution after ultrasonic with 0.22 μm, obtain the Nano silver solution clarified.As shown in Figure 5, as can be seen from Figure 5, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Embodiment 6:
Cultured silk algae algae liquid is added in centrifuge tube and is placed in 5000rpm on centrifuge centrifugal 5 minutes, by weight in wet base 1g frustule precipitation be resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, power output is 200 watts, ultrasonic 1min, interval 1min, repeats 5 times.Centrifugal, remove algae precipitation, liquor argenti nitratis ophthalmicus is added in 20ml supernatant, make the final concentration of silver ion be 5 μ g/mL, be placed in 20 DEG C of oscillating reactions 1h on shaking bath, then reactant liquor carried out ultrasonication under condition of ice bath, power output is 200 watts, ultrasonic 1min, interval 1min, repeats 5 times.By the membrane filtration of the solution after ultrasonic with 0.1 μm, obtain the Nano silver solution clarified.As shown in Figure 6, as can be seen from Figure 6, the Nano silver solution of the present embodiment is Nano silver solution to the Electronic Speculum figure of this Nano silver solution really.
Claims (3)
1. the method utilizing micro-algae to prepare Nano Silver, it is characterized in that, by centrifugal to chlorella, silk algae or Scenedesmus quadricauda algae liquid, frustule precipitation is resuspended in the water of sterilizing, under being placed in ultrasonic wave, ice bath is broken, centrifugal, remove algae precipitation, in the algae liquid of supernatant, add silver nitrate, the final concentration of silver ion is 5-100 μ g/mL, be placed in 20 ~ 40 DEG C of water-bath concussion reaction 1-5h again, then through ultrasonic process, membrane filtration, filtrate is Nano silver solution.
2. method according to claim 1, is characterized in that, described in be placed in the broken and ultrasonic process of ice bath under ultrasonic wave, its ultrasonic power output is 150-200W, broken 3-10min.
3. method according to claim 1, is characterized in that, described filtration is the membrane filtration with aperture 0.1-0.22 μm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109569831A (en) * | 2019-01-17 | 2019-04-05 | 新疆隆博叶希丽生态环保有限公司 | A kind of breaking method of desert frustule |
| CN113618078A (en) * | 2021-08-05 | 2021-11-09 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
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| CN1395829A (en) * | 2002-08-06 | 2003-02-12 | 中国地质大学（武汉） | Antibacterial nano silver powder using algae as carrier and its preparing process |
| CN101003784A (en) * | 2006-12-27 | 2007-07-25 | 天津商学院 | Ultrasonic method for breaking up micro alga cells |
| CN103360510A (en) * | 2013-07-31 | 2013-10-23 | 青岛海之林生物科技开发有限公司 | Production method for alginate specialized for welding |
| CN104194004A (en) * | 2014-01-27 | 2014-12-10 | 武汉纺织大学 | A green synthesis method of a nanometer silver-sodium alginate composition |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395829A (en) * | 2002-08-06 | 2003-02-12 | 中国地质大学（武汉） | Antibacterial nano silver powder using algae as carrier and its preparing process |
| CN101003784A (en) * | 2006-12-27 | 2007-07-25 | 天津商学院 | Ultrasonic method for breaking up micro alga cells |
| CN103360510A (en) * | 2013-07-31 | 2013-10-23 | 青岛海之林生物科技开发有限公司 | Production method for alginate specialized for welding |
| CN104194004A (en) * | 2014-01-27 | 2014-12-10 | 武汉纺织大学 | A green synthesis method of a nanometer silver-sodium alginate composition |
Non-Patent Citations (1)
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| 杨芳等: "藻蓝蛋白原位还原Ag(Ⅰ)与纳米Ag(0)形成动态过程的谱学研究", 《光谱学与光谱分析》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109569831A (en) * | 2019-01-17 | 2019-04-05 | 新疆隆博叶希丽生态环保有限公司 | A kind of breaking method of desert frustule |
| CN113618078A (en) * | 2021-08-05 | 2021-11-09 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
| CN113618078B (en) * | 2021-08-05 | 2023-01-31 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
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Address after: Guangzhou City, Guangdong province 510070 martyrs Road No. 100 building No. 56 Patentee after: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Address before: Guangzhou City, Guangdong province 510070 martyrs Road No. 100 Patentee before: GUANGDONG INSTITUTE OF MICROBIOLOGY |
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