CN104525965B - A kind of method utilizing microalgae to prepare nanometer silver - Google Patents
A kind of method utilizing microalgae to prepare nanometer silver Download PDFInfo
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- CN104525965B CN104525965B CN201410830502.7A CN201410830502A CN104525965B CN 104525965 B CN104525965 B CN 104525965B CN 201410830502 A CN201410830502 A CN 201410830502A CN 104525965 B CN104525965 B CN 104525965B
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Abstract
The invention discloses a kind of straightforward procedure utilizing microalgae to prepare nanometer silver.It is chlorella or silk algae or Scenedesmus quadricauda algae solution to be centrifuged respectively, frustule precipitation is resuspended in the deionized water of sterilizing, being placed under ultrasound wave ice bath and crush, output is 150-200W, broken 3-10min, centrifugal, removing algae precipitation, add silver nitrate in algae solution, the concentration of silver ion is 5-100 μ g/mL, it is placed in 20-40 DEG C of shaking bath concussion reaction 1-5h, obtains nanometer silver solution then through supersound process, membrane filtration.The present invention utilizes microalgae to prepare nano silver particles, and this preparation method is simple and easy to do, workable, with low cost, and preparation process will not discharge poisonous and harmful substance to environment, is a not only simple but also nano silver preparation technology for environmental protection.
Description
Technical field:
The invention belongs to biological technical field, be specifically related to a kind of method utilizing microalgae to prepare nanometer silver.
Background technology:
As far back as the ancient times, argent to having antibacterial and bactericidal action, and has the antibacterial activity of wide spectrum with regard to recognized.In recent years, the New research progress to metal nanoparticle, promote nanometer silver in the application in antibacterial field, research finds that the nanometer silver prepared by multiple method all has efficient antibacterial activity.Nanometer silver is referred to as the antibacterial of 21st century a new generation, and it has wide spectrum, efficient anti-microbial property, and nanometer silver was widely used already as anti-biotic material, as being used in burn dressing, operating-table, water purification installation and some armarium etc..Based on the anti-microbial property that nanometer silver is excellent, it has a extensive future, and has the market demand widely in consumer goods industries, medicine and hygiene fields, building materials field, electronics industry etc..
The preparation method of nano silver particles is a lot, it is mainly the chemical methodes such as solution phase chemical reduction, electrochemical process, photochemical reduction, with the reduction physical method such as ball-milling method, using vaporization condensation process, atomization, wherein especially relatively common with chemical reduction method, it also requires strictly control reaction condition, the requirement of technology of preparing is higher.
Summary of the invention:
It is an object of the invention to provide a kind of simple and easy to do, workable, with low cost, and preparation process will not discharge poisonous and harmful substance to environment, compares the method utilizing microalgae to prepare nanometer silver of environmental protection.
The present invention utilizes the method that microalgae prepares nanometer silver, it is characterized in that, chlorella, silk algae or Scenedesmus quadricauda algae solution are centrifuged, frustule precipitation is resuspended in the water of sterilizing, it is placed under ultrasound wave ice bath and crushes, centrifugal, remove algae precipitation, in the algae solution of supernatant, add silver nitrate, the final concentration of 5-100 μ g/mL of silver ion, being placed in 20-40 DEG C of water-bath concussion reaction 1-5h, then sonicated, membrane filtration again, filtrate is nanometer silver solution.
Preferably, described in the broken and supersound process that is placed under ultrasound wave ice bath, its ultrasonic output is 150-200W, broken 3-10min.
Described filters the membrane filtration preferably with 0.1-0.22 μm of aperture.
The present invention utilizes microalgae to prepare nano silver particles, and this preparation method is simple and easy to do, workable, with low cost, and preparation process will not discharge poisonous and harmful substance to environment, is a not only simple but also nano silver preparation technology for environmental protection.
Accompanying drawing illustrates:
Fig. 1-Fig. 6 is the transmission electron microscope picture of the nanometer silver solution of embodiment 1-6 respectively.
Detailed description of the invention:
Following example are further illustrating the present invention, rather than limitation of the present invention.
Embodiment 1:
Be added in centrifuge tube by cultured chlorella algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 1g microalgae be resuspended in 20mL deionized water, and under condition of ice bath ultrasonication frustule, ultrasonic output is 150 watts, ultrasonic 1min, interval 1min, repeat 5 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, make the final concentration of 10 μ g/mL of silver ion, be placed on shaking bath 30 DEG C of oscillating reactions 5h, then reactant liquor is carried out under condition of ice bath ultrasonication, output is 150 watts, ultrasonic 1min, interval 1min, repeat 5 times.By the membrane filtration with 0.22 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution is as it is shown in figure 1, it will be seen from figure 1 that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Embodiment 2:
Be added in centrifuge tube by cultured chlorella algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 2g frustule precipitation be resuspended in the deionized water of 20ml sterilizing, and under condition of ice bath ultrasonication frustule, output is 200 watts, ultrasonic 1min, interval 1min, repeat 10 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, make the final concentration of 20 μ g/mL of silver ion, be placed on shaking bath 40 DEG C of oscillating reactions 5h, then reactant liquor is carried out under condition of ice bath ultrasonication, output is 150 watts, ultrasonic 1min, interval 1min, repeat 3 times.By the membrane filtration with 0.22 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution is as in figure 2 it is shown, figure it is seen that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Embodiment 3:
Be added in centrifuge tube by cultured chlorella algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 5g frustule precipitation be resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, output is 200 watts, ultrasonic 1min, interval 1min, repeat 5 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, the concentration making silver ion is 50 μ g/mL, is placed on shaking bath 20 DEG C of oscillating reactions 5h, and then reactant liquor carries out ultrasonication under condition of ice bath, output is 150 watts, ultrasonic 1min, interval 1min, repeat 10 times.By the membrane filtration with 0.22 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution is as it is shown on figure 3, from figure 3, it can be seen that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Embodiment 4:
Be added in centrifuge tube by cultured chlorella algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 5g frustule precipitation be resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, output is 200 watts, ultrasonic 1min, interval 1min, repeat 3 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, the concentration making silver ion is 100 μ g/mL, is placed on shaking bath 30 DEG C of oscillating reactions 5h, and then reactant liquor carries out ultrasonication under condition of ice bath, output is 150 watts, ultrasonic 1min, interval 1min, repeat 5 times.By the membrane filtration with 0.22 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution as shown in Figure 4, from fig. 4, it can be seen that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Embodiment 5:
It is added in centrifuge tube by cultured Scenedesmus quadricauda algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 1g frustule precipitation is resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, output is 150 watts, ultrasonic 1min, interval 1min, repeats 5 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, make the final concentration of 10 μ g/mL of silver ion, be placed on shaking bath 20 DEG C of oscillating reactions 5h, then reactant liquor is carried out under condition of ice bath ultrasonication, output is 150 watts, ultrasonic 1min, interval 1min, repeat 5 times.By the membrane filtration with 0.22 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution is as it is shown in figure 5, from fig. 5, it can be seen that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Embodiment 6:
Be added in centrifuge tube by cultured silk algae algae solution to be placed on centrifuge 5000rpm centrifugal 5 minutes, weight in wet base 1g frustule precipitation be resuspended in the deionized water of 20mL sterilizing, and under condition of ice bath ultrasonication frustule, output is 200 watts, ultrasonic 1min, interval 1min, repeat 5 times.Centrifugal, remove algae precipitation, silver nitrate solution is added in 20ml supernatant, make the final concentration of 5 μ g/mL of silver ion, be placed on shaking bath 20 DEG C of oscillating reactions 1h, then reactant liquor is carried out under condition of ice bath ultrasonication, output is 200 watts, ultrasonic 1min, interval 1min, repeat 5 times.By the membrane filtration with 0.1 μm of the solution after ultrasonic, obtain the nanometer silver solution of clarification.The Electronic Speculum figure of this nanometer silver solution as shown in Figure 6, from fig. 6, it can be seen that the nanometer silver solution of the present embodiment is nanometer silver solution really.
Claims (3)
1. one kind utilizes the method that microalgae prepares nanometer silver, it is characterized in that, chlorella, silk algae or Scenedesmus quadricauda algae solution are centrifuged, frustule precipitation is resuspended in the water of sterilizing, it is placed under ultrasound wave ice bath and crushes, centrifugal, remove algae precipitation, in the algae solution of supernatant, add silver nitrate, the final concentration of 5-100 μ g/mL of silver ion, being placed in 20～40 DEG C of water-baths concussion reaction 1-5h, then sonicated, membrane filtrations again, filtrate is nanometer silver solution.
2. method according to claim 1, it is characterised in that described in the broken and supersound process that is placed under ultrasound wave ice bath, its ultrasonic output is 150-200W, broken 3-10min.
3. method according to claim 1, it is characterised in that described filtration is with the membrane filtration in 0.1-0.22 μm of aperture.
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| CN109569831A (en) * | 2019-01-17 | 2019-04-05 | 新疆隆博叶希丽生态环保有限公司 | A kind of breaking method of desert frustule |
| CN113618078B (en) * | 2021-08-05 | 2023-01-31 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
Citations (4)
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|---|---|---|---|---|
| CN1395829A (en) * | 2002-08-06 | 2003-02-12 | 中国地质大学（武汉） | Antibacterial nano silver powder using algae as carrier and its preparing process |
| CN101003784A (en) * | 2006-12-27 | 2007-07-25 | 天津商学院 | Ultrasonic method for breaking up micro alga cells |
| CN103360510A (en) * | 2013-07-31 | 2013-10-23 | 青岛海之林生物科技开发有限公司 | Production method for alginate specialized for welding |
| CN104194004A (en) * | 2014-01-27 | 2014-12-10 | 武汉纺织大学 | A green synthesis method of a nanometer silver-sodium alginate composition |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395829A (en) * | 2002-08-06 | 2003-02-12 | 中国地质大学（武汉） | Antibacterial nano silver powder using algae as carrier and its preparing process |
| CN101003784A (en) * | 2006-12-27 | 2007-07-25 | 天津商学院 | Ultrasonic method for breaking up micro alga cells |
| CN103360510A (en) * | 2013-07-31 | 2013-10-23 | 青岛海之林生物科技开发有限公司 | Production method for alginate specialized for welding |
| CN104194004A (en) * | 2014-01-27 | 2014-12-10 | 武汉纺织大学 | A green synthesis method of a nanometer silver-sodium alginate composition |
Non-Patent Citations (1)
| Title |
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| 藻蓝蛋白原位还原Ag(Ⅰ)与纳米Ag(0)形成动态过程的谱学研究;杨芳等;《光谱学与光谱分析》;20070131;第27卷(第01期);23-27页 * |
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