CN1390946A - Process for preparing isoflavone galactoside - Google Patents

Abstract

A process for preparing isoflavone galactoside from the husk of sorghum grain includes such steps as washing raw material, enzymolyzing cellulose, extracting, filtering, concentrating, enzymolyzing pectin, depositing, separating and drying. Its advantage is high output rate (5-7 kg/100kg).

Description

Process for preparing isoflavone galactoside

The present invention relates to a kind of process for preparing isoflavone galactoside, particularly a kind of preparation technology who from black sorghum husk, extracts isoflavone galactoside.

According to " Dictionary of Chinese Pharmacy " record, Chinese sorghum also claims millet rice, and property is sweet, cold, nontoxic.Effect with the stomach invigorating the spleen is arranged, as relaxing tonic, can beneficial gas and in, suitable spleen, sharp stomach; Function of LI Shi-Zhen Compendium of Material Medica opinion millet rice day cool blood is arranged, detoxifying, relieve summer heat, quenching the thirst or the like.

Since ancient times, the Chinese sorghum of China cultivation has multiple kinds such as white Chinese sorghum, yellow Chinese sorghum, blue or green Chinese sorghum, red sorghum, but among the people have only with red sorghum do folk prescription treatment diabetes, hypertension and hyperlipidemia, its major cause is because wherein contain the Chinese sorghum red pigment.The main component that studies have shown that the Chinese sorghum red pigment is isoflavone galactoside.Though this material has the obvious treatment effect for diseases such as diabetes, hypertension, up to the present still do not have effective industrial process and be used to extract isoflavone galactoside, therefore influenced its use greatly in pharmaceutical industries.

The purpose of this invention is to provide a kind of preparation technology who from black sorghum husk, extracts isoflavone galactoside, to solve the fewer but better problem that to go into medicinal substances in the sorghum husk.

The object of the present invention is achieved like this: it comprises following technological process:

(1), raw material cleans

The black sorghum husk was then soaked for 5-8 time 3-5 hour with the room temperature flushing with clean water;

(2), enzymolysis Mierocrystalline cellulose

By (1) the black sorghum husk that cleans up add the cellulase of 5-10PPM, its amount is spilling water degree of being not to soak into shell, under 20-25 ℃ of condition enzymolysis 2-3 hour;

(3), extract filtration

With the sorghum husk temperature after the cellulase decomposition is 25-45 ℃, and the pH value is that the alkaline water of 9-10 stirred lixiviate 3-5 hour down, and the heavy ratio with the vat liquor volume of its shell is W/V=1: 10; Filter with 100 eye mesh screens, shell and vat liquor are separated, abandon shell and stay vat liquor;

(4), filtering and concentrating

With vat liquor before concentrating with after the filtration of 200 eye mesh screens, filtrate is concentrating under reduced pressure under 80 ℃ of conditions in temperature, and filter liquor concentration is reached about Brix10, the enzyme that goes out simultaneously work;

(5), enzymolysis pectin

With concentrated solution is the polygalacturonase that the extracting solution of Brix10 adds 0.5-1PPM, and making its extract concentration is Brix8-9 degree of being, under 20-25 ℃ of temperature enzymolysis 1-1.5 hour;

(6), settlement separate

Through being 3-4 with modified its pH that makes of the extracting solution behind the pectinase enzymatic hydrolysis, at room temperature sedimentation 3-5 hour; Then, sediment is removed solidity impurity with the centrifugal elder generation of tubular-bowl centrifuge, and the back is centrifugal with butterfly sheet whizzer, discharges excessive moisture;

(7), finished product drying

Is 220-245 ℃ with centrifugal sediment in inlet temperature, and temperature out is 100-110 ℃ and carries out spraying drying, promptly gets the pulverous natural isoflavone galactoside of garnet.

By above-mentioned technology, per 100 kilograms of raw materials can be carried 5-7 kilogram isoflavone galactoside, and this material is crude substance sheerly, carries out the food safety toxicological experiment through Liaoning Province's Food Hygiene Surveillance check and assert nontoxic hereditary-less toxicity material (No. 199416), can use in healthcare products, medicine, drink and food.

Its main physics and chemistry and sanitary index are as follows:

Product appearance: garnet is Powdered

Solvability: can be water-soluble

Thermotolerance: 100-300 ℃

Arsenic (As) mg/kg :≤0.25

Plumbous (Pb) mg/kg :≤0.20

Cadmium (Cd) mg/kg: do not detect

Copper (Cu) mg/kg :≤0.9

Zinc (Zn) mg/kg :≤16.8

Mercury (Hg) mg/kg: do not detect

Sterilant mg/kg: do not detect

Total number of bacterial colony :≤10

Below in conjunction with specific embodiment in detail the present invention is described in detail.

Embodiment 1

Its preparation process is as follows:

(1), raw material cleans

The black sorghum husk was then soaked for 5-8 time 4.5 hours with the room temperature flushing with clean water, remove gentle acid substance soluble in water in the raw material;

(2), enzymolysis Mierocrystalline cellulose

The black sorghum husk that cleans up that is got by (1) adds the cellulase of 6PPM, it measures to soak into shell not spilling water degree of being, enzymolysis is 2.5 hours under 20-25 ℃ of condition, decomposes Mierocrystalline cellulose in the h wall with cellulase, to increase the extracted amount of isoflavone galactoside;

(3), extract filtration

Sorghum husk temperature after cellulase decomposed is 30 ℃, and pH value is that 9.2 alkaline water stirs following lixiviate 3 hours, and its shell weight is W/V=1 with the ratio of vat liquor volume: 10; Filter with 100 eye mesh screens, shell and vat liquor are separated, abandon shell and stay vat liquor;

(4), filtering and concentrating

With vat liquor before concentrating with after the filtration of 200 eye mesh screens, filtrate is concentrating under reduced pressure under 80 ℃ of conditions in temperature, and filter liquor concentration is reached about Brix10, the enzyme that goes out simultaneously work;

(5), enzymolysis pectin

With concentrated solution is the polygalacturonase of the extracting solution adding 0.5PPM of Brix10, making its extract concentration is Brix8, enzymolysis is 1.5 hours under 23 ℃ of temperature, to decompose the pectin substance in the extracting solution, the content that improves product purity, solvability and increase soluble cellulose is in order to improving hypoglycemic, lipopenicillinase and antihypertensive effect;

(6), settlement separate

Through being 3.5 with modified its pH that makes of the extracting solution behind the pectinase enzymatic hydrolysis, sedimentation at room temperature 3.5 hours; Sediment is removed solidity impurity with the centrifugal elder generation of tubular-bowl centrifuge, and the back is centrifugal with butterfly sheet whizzer, discharges excessive moisture;

(7), finished product drying

Is 220-245 ℃ with centrifugal sediment in inlet temperature, and temperature out is 100-110 ℃ and carries out spraying drying, promptly gets the pulverous natural isoflavone galactoside of garnet.

Embodiment 2

Its preparation process is as follows:

(1), raw material cleans

The black sorghum husk was then soaked for 5-8 time 4 hours with the room temperature flushing with clean water, remove gentle acid substance soluble in water in the raw material;

(2), enzymolysis Mierocrystalline cellulose

The black sorghum husk that cleans up that is got by (1) adds the cellulase of 9PPM, and it measures to soak into shell not spilling water degree of being, enzymolysis is 3 hours under 25 ℃ of conditions, decomposes Mierocrystalline cellulose in the h wall with cellulase, to increase the extracted amount of isoflavone galactoside;

(3), extract filtration

Sorghum husk temperature after cellulase decomposed is 25 ℃, and pH value is that 9.8 alkaline water stirs following lixiviate 3 hours, and its shell weight is W/V=1 with the ratio of vat liquor volume: 10; Filter with 100 eye mesh screens, shell and vat liquor are separated, abandon shell and stay vat liquor;

(4), filtering and concentrating

With vat liquor before concentrating with after the filtration of 200 eye mesh screens, filtrate is concentrating under reduced pressure under 80 ℃ of conditions in temperature, and filter liquor concentration is reached about Brix10, the enzyme that goes out simultaneously work;

(5), enzymolysis pectin

With concentrated solution is the polygalacturonase of the extracting solution adding 1PPM of Brix10, making its extract concentration is Brix9, enzymolysis is 1 hour under 25 ℃ of temperature, to decompose the pectin substance in the extracting solution, the content that improves product purity, solvability and increase soluble cellulose is in order to improving hypoglycemic, lipopenicillinase and antihypertensive effect;

(6), settlement separate

Through being 4 with modified its pH that makes of the extracting solution behind the pectinase enzymatic hydrolysis, sedimentation at room temperature 5 hours; Sediment is removed solidity impurity with the centrifugal elder generation of tubular-bowl centrifuge, and the back is centrifugal with butterfly sheet whizzer, discharges excessive moisture;

(7), finished product drying

Is 220-245 ℃ with centrifugal sediment in inlet temperature, and temperature out is 100-110 ℃ and carries out spraying drying, promptly gets the pulverous natural isoflavone galactoside of garnet.

Claims (1)

1, a kind of process for preparing isoflavone galactoside is characterized in that: it comprises following technological process:

(1), raw material cleans

The black sorghum husk was then soaked for 5-8 time 3-5 hour with the room temperature flushing with clean water;

(2), enzymolysis Mierocrystalline cellulose

By (1) the black sorghum husk that cleans up add the cellulase of 5-10PPM, its amount is spilling water degree of being not to soak into shell, under 20-25 ℃ of condition enzymolysis 2-3 hour;

(3), extract filtration

With the sorghum husk temperature after the cellulase decomposition is 25-45 ℃, and the pH value is that the alkaline water of 9-10 stirred lixiviate 3-5 hour down, and the heavy ratio with the vat liquor volume of its shell is W/V=1: 10; Filter with 100 eye mesh screens, shell and vat liquor are separated, abandon shell and stay vat liquor;

(4), filtering and concentrating

With vat liquor before concentrating with after the filtration of 200 eye mesh screens, filtrate is concentrating under reduced pressure under 80 ℃ of conditions in temperature, and filter liquor concentration is reached about Brix10, the enzyme that goes out simultaneously work;

(5), enzymolysis pectin

With concentrated solution is the polygalacturonase that the extracting solution of Brix10 adds 0.5-1PPM, and making its extract concentration is Brix8-9 degree of being, under 20-25 ℃ of temperature enzymolysis 1-1.5 hour;

(6), settlement separate

Through being 3-4 with modified its pH that makes of the extracting solution behind the pectinase enzymatic hydrolysis, at room temperature sedimentation 3-5 hour; Then, sediment is removed solidity impurity with the centrifugal elder generation of tubular-bowl centrifuge, and the back is centrifugal with butterfly sheet whizzer, discharges excessive moisture;

(7), finished product drying

Is 220-245 ℃ with centrifugal sediment in inlet temperature, and temperature out is 100-110 ℃ and carries out spraying drying, promptly gets the pulverous natural isoflavone galactoside of garnet.