CN1371598A - Mountain poplar group cultivation nursery garden rooting method - Google Patents
Mountain poplar group cultivation nursery garden rooting method Download PDFInfo
- Publication number
- CN1371598A CN1371598A CN01106230A CN01106230A CN1371598A CN 1371598 A CN1371598 A CN 1371598A CN 01106230 A CN01106230 A CN 01106230A CN 01106230 A CN01106230 A CN 01106230A CN 1371598 A CN1371598 A CN 1371598A
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- CN
- China
- Prior art keywords
- aspen
- cultivating seedling
- tissue cultivating
- mycorrhizal
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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- Cultivation Of Plants (AREA)
Abstract
The present invention provides a novel method for producing nursery garden by biological technology, and is characterized by that the normal region soil of mountain poplar is used to inoculate tissue culture seedling of moutain poplar to obtain mountain poplar mycorrhizic tissue, culture seedling of infective extomycorrhize and VA mycorrhiza, at the same time the pure-cultured humicolous hollow sphaeroid bacterium and colour bean puffball are used to respectively inoculate moutain poplar tissue culture seedling so as to obtain the invented mountain poplar mycorrhizic nursery stock. Said invention can raise quality of nursery stock, and can raise plant percent and can raise young growth speed.
Description
The present invention relates to a kind of novel method of producing nursery stock, particularly a kind of novel method that relates to the production nursery stock of biological technical field.
Aspen (Populus davidiana Dove) is the chief species of forest zone, China northeast secondary forest, its area coverage is bigger, and root evil ability is strong, can form to invade to move standing forest, can grow in dense stand, be again the pioneer tree species of the renewal of forest on abandoned land, cutting blank and the burned area.But aspen planting out survival rate is very low, and the aspen forest plantation that grows into forest is at present seldom seen.Though China forestry scientific research personnel fails to solve the problem that aspen tissue cultivating seedling planting out is not grown into forest all the time through research and the afforestation practice of more than ten years.But show that according to interrelated data a key factor of restriction aspen tissue cultivating seedling planting out survival rate is a moisture problem.
The object of the present invention is to provide a kind of mycorhiza bacterium inoculation aspen tissue cultivating seedling that has than the strong drought resistant function, make it become the strong Mycorrhizal nursery stock of drought-resistant ability, start with, solve aspen tissue cultivating seedling planting out problem from solving aspen tissue cultivating seedling self water utilization problem.Still do not have report about using aspen Mycorrhizal tissue cultivating seedling afforestation aspect China, this invention will provide a new way for successful Application aspen tissue cultivating seedling afforestation.
Advantage of the present invention is: use this technology and can improve seedling quality, improve the drought resistance of nursery stock, thereby improve aspen tissue cultivating seedling survival rate; Promote nursery stock to the particularly absorption of insoluble phosphorus of nutrient, improve young growth early growth speed, grow into forest ahead of time.
Below embodiments of the invention are described in further detail.
Obtain the aspen tissue cultivating seedling by the method for utilizing Plant Tissue Breeding.The tissue culture of aspen is that the application constituent is KNO
3, NH
4NO
3, MgSO
47H
2O, Ca (NO
3)
2, K
2SO
4, CaCl
22H
2O, KH
2PO
4, MnSO
44H
2O, ZnSO
47H
2O, H
3BO
3, CuSO
45H
2O, KI, Na
2MoO
42H
2The WP medium of O, EDTA-Fe, nicotinic acid, inositol, glycine, thiamine hydrochloride, puridoxine hydrochloride, agar, sucrose, 6-BA, NAA and IBA carries out.The used medium in each period of incubation specifically comprises following three types:
Differential medium: WP+6-BA0.8mg/L+NAA0.02mg/L
Subculture medium: WP+6-BA0.5mg/L+NAA0.1mg/L
Root media: WP+IBA0.8mg/L
Set up the aspen regenerative system by above method, obtain the aspen tissue cultivating seedling.
With above mountain poplar group cultivation nursery garden rooting, obtain following Mycorrhizal nursery stock by the Mycorrhizal technology:
A, field acquisition aspen suitable habitat soil inoculation aspen tissue cultivating seedling obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza and VA mycorhiza.
B, with the autochthonal empty coccus of pure culture (Cenococcum geophilum) inoculation aspen tissue cultivating seedling, obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza.
C, with the colored beans Lasiosphaera fenzliis of pure culture (Pisolithus tinctorius) inoculation aspen tissue cultivating seedling, obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza.
Above tissue cultivating seedling adopts bore and highly is the nutritive cube cultivation of 10cm, infects success after one month, obtains Mycorrhizal aspen tissue cultivating seedling, promptly can be used for afforestation after 80 days.
Claims (1)
1, a kind of mountain poplar group cultivation nursery garden rooting method is characterized in that:
Utilize the method for Plant Tissue Breeding to obtain the aspen tissue cultivating seedling, the tissue culture of aspen is that the application constituent is KNO
3, NH
4NO
3, MgSO
47H
2O, Ca (NO
3)
2, K
2SO
4, CaCl
22H
2O, KH
2PO
4, MnSO
44H
2O, ZnSO
47H
2O, H
3BO
3, CuSO
45H
2O, KI, Na
2MoO
42H
2The WP medium of O, EDTA-Fe, nicotinic acid, inositol, glycine, thiamine hydrochloride, puridoxine hydrochloride, agar, sucrose, 6-BA, NAA and IBA carries out.The used medium in each period of incubation specifically comprises following three types:
Differential medium: WP+6-BA0.5-0.8mg/L+NAA0.01-0.05mg/l
Subculture medium: WP+6-BA0.5-1mg/L+NAA0.05-0.2mg/L
Root media: WP+IBA0.8-1mg/L
Set up the aspen regenerative system by above method, obtain the aspen tissue cultivating seedling.
With above mountain poplar group cultivation nursery garden rooting, obtain following three kinds of different Mycorrhizal nursery stocks by mycorrhizal method:
A, field acquisition aspen suitable habitat soil inoculation aspen tissue cultivating seedling obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza and VA mycorhiza.
B, with the autochthonal empty coccus of pure culture (Cenococcum geophilum) inoculation aspen tissue cultivating seedling, obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza.
C, with the colored beans Lasiosphaera fenzliis of pure culture (Pisolithus tinctorius) inoculation aspen tissue cultivating seedling, obtain infecting the aspen Mycorrhizal tissue cultivating seedling of ectotrophic mycorrhiza.
Above tissue cultivating seedling adopts bore and highly is the nutritive cube cultivation of 10cm, infects success after one month, obtains Mycorrhizal aspen tissue cultivating seedling, promptly can be used for afforestation after 60-80 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01106230A CN1371598A (en) | 2001-02-28 | 2001-02-28 | Mountain poplar group cultivation nursery garden rooting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01106230A CN1371598A (en) | 2001-02-28 | 2001-02-28 | Mountain poplar group cultivation nursery garden rooting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1371598A true CN1371598A (en) | 2002-10-02 |
Family
ID=4655269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN01106230A Pending CN1371598A (en) | 2001-02-28 | 2001-02-28 | Mountain poplar group cultivation nursery garden rooting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1371598A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246700A (en) * | 2011-08-18 | 2011-11-23 | 西南林业大学 | Tissue culture method for populus yunnanensis Dode with tender stem as explant |
| CN110367102A (en) * | 2019-05-31 | 2019-10-25 | 中南林业科技大学 | A kind of method of chinquapin regeneration plant and Applying Ectomycorrhizal Fungi symbiosis |
-
2001
- 2001-02-28 CN CN01106230A patent/CN1371598A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246700A (en) * | 2011-08-18 | 2011-11-23 | 西南林业大学 | Tissue culture method for populus yunnanensis Dode with tender stem as explant |
| CN110367102A (en) * | 2019-05-31 | 2019-10-25 | 中南林业科技大学 | A kind of method of chinquapin regeneration plant and Applying Ectomycorrhizal Fungi symbiosis |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |