CN1358999A - Automatic focus regulator for laser cofocal scanner - Google Patents
Automatic focus regulator for laser cofocal scanner Download PDFInfo
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- CN1358999A CN1358999A CN 02100262 CN02100262A CN1358999A CN 1358999 A CN1358999 A CN 1358999A CN 02100262 CN02100262 CN 02100262 CN 02100262 A CN02100262 A CN 02100262A CN 1358999 A CN1358999 A CN 1358999A
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- 239000000523 sample Substances 0.000 claims abstract description 13
- 230000003287 optical effect Effects 0.000 claims abstract description 12
- 239000012472 biological sample Substances 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 6
- 238000000018 DNA microarray Methods 0.000 claims description 8
- 230000003321 amplification Effects 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 230000005622 photoelectricity Effects 0.000 claims description 2
- 201000009310 astigmatism Diseases 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- 230000015654 memory Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
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Abstract
激光共焦扫描仪的自动调焦装置属于生物、医疗、科学仪器用的光电探测装置领域,其特征在于,它含有:光学头,它依次由激光器、针孔、透镜、物镜和样品构成的入射光路,由上述样品和物镜、分光镜、像散透镜和四象限光电探测器构成的调焦用反射光路以及依次由上述样品和物镜、二向色分光镜、透镜、针孔和光电倍增管构成的检测用反射光路;所述的调焦控制电路是以数字信号处理器、计算机和执行电机为主构成的数字控制电路;调焦机构是与执行电路的输出轴相连的丝杠—螺母式调焦机构,同时设有光电控制式限位开关。它可以用自动调焦的方式检测生物样品,调焦范围大,精度高。
The automatic focusing device of a laser confocal scanner belongs to the field of photoelectric detection devices for biological, medical, and scientific instruments. It is characterized in that it contains: an optical head, which in turn consists of a laser, a pinhole, a lens, an objective lens, and a sample. The optical path is a focusing reflection optical path composed of the above-mentioned sample and objective lens, a beam splitter, an astigmatism lens and a four-quadrant photodetector, and is composed of the above-mentioned sample and objective lens, a dichroic beam splitter, a lens, a pinhole and a photomultiplier tube in turn. The reflective light path for detection; the focusing control circuit is a digital control circuit mainly composed of a digital signal processor, a computer and an execution motor; the focusing mechanism is a screw-nut type adjustment connected to the output shaft of the execution circuit The focusing mechanism is also equipped with a photoelectrically controlled limit switch. It can detect biological samples using automatic focusing, with a large focusing range and high precision.
Description
Technical field
The Electro-Optical Sensor Set field that the automatic focusing device of laser cofocal scanner belongs to is biological, medical treatment, scientific instrument are used.
Background technology
Laser cofocal scanner is compared with simple microscope, has higher resolution, is widely used in biology, life, medical treatment and the scientific instrument.Existing focusing technology and device, they are researched and developed at different technical fields, can not be applicable to laser cofocal scanner fully.As the patent No. is ZL98229444.1, the Chinese utility model patent of " focus control of lithography machine of submicron " by name discloses a kind of litho machine focus control, it is characterized by: the imaging of mark is through being carved object, little object lens, behind the catoptron, be divided into two-way by spectroscope, one road catoptric imaging is in the four-quadrant receiver, another Reuter penetrates through imagery of cylindrical mirror in ccd image sensor, two-way output defocus signal and target amount of bias are sent into single-chip microcomputer, the control piezoelectric ceramics promotes flexible hinge, drive quilt and carve the motion that object is made big displacement or micrometric displacement, reach the target position of focal plane.It does not detect and uses reflected light path, can not be used for the laser cofocal scanner of detection of biological chip.
Goal of the invention
The object of the present invention is to provide a kind of automatic focusing device that can be used for the laser cofocal scanner that biochip test uses.
The invention is characterized in: described optical scanning head contains the input path that is made of laser instrument, pin hole, lens, object lens and sample successively, and the focusing that is made of above-mentioned sample and object lens, spectroscope, astigmatic lens and four-quadrant photo detector is with reflected light path and the detection reflected light path that is made of above-mentioned sample and object lens, dichroic beamsplitter, lens, pin hole and photomultiplier successively successively; Described focusing control circuit is to serve as the digital control circuit of main formation with digital signal processing circuit and actuating motor; Described focus adjusting mechanism is the leading screw-nut type mechanical focusing mechanism of joining with the actuating motor output shaft.The amplification that described focusing control circuit is linked to each other with the output terminal of above-mentioned four-quadrant photo detector by its input end successively, filtering and A/D change-over circuit, digital signal processor DSP, photoelectricity isolation, D/A conversion, amplifying circuit and actuating motor serial connection form.Described digital signal processor DSP links to each other with computing machine.Described leading screw-nut type mechanical focusing mechanism is contained: motor base, support and the fixing mounting plate of slide block are housed, pass leading screw and nut that motor base, support are connected with the output shaft of motor by shaft joint, link to each other with nut and be connected one and can move and opposite side is equipped with the removable bottom that optical scanning head is a camera lens, be contained in the photoelectric sensor on the said fixing slide block with respect to the said fixing slide block.
Use proof: it has reached intended purposes.
Description of drawings
Fig. 1～Fig. 2: the index path of two embodiment of optical scanning head in the laser cofocal scanner.
Fig. 3: the circuit theory block scheme of focusing control circuit.
Fig. 4: the mechanical principle block diagram of leading screw-nut type focus adjusting mechanism.
Embodiment
Ask for an interview Fig. 1.Laser instrument 1 is near pin hole 2, be placed on the front focal plane of lens 3, laser projects lens 3 through pin hole 2 and becomes directional light, and object lens 6 vertical irradiations of process spectroscope 4, dichroic beamsplitter 5, large-numerical aperture excite the biological sample on the biochip 8 to send fluorescence to biochip 8.Reflected light is made up of the incident laser of fluorescence that ejects from biological sample and reflection.Reflected light becomes directional light through object lens 6, after arriving dichroic beamsplitter 5, be divided into two-beam: the fluorescence that first bundle only ejects from biological sample is reflexed on the lens 3 by dichroic beamsplitter 5, image in pin hole 2, detected by immediate photomultiplier tube 10; Second bundle is laser light reflected from the biological sample only, during by dichroic beamsplitter 5, is transmitted on the spectroscope 4, reflexes on the astigmatic lens 11, images on the four-quadrant photo detector 12.Suitably select the focusing parameter of the both direction of astigmatic lens 11, can obtain certain focusing range and certain focusing accuracy.The object lens 6 of large-numerical aperture of focusing usefulness be fixed on can portable plate 7 by driven by motor on, biochip 8 is placed on the sample retainer 9.
Goodbye Fig. 2.The laser of part incident sees through biological sample 8, become directional light through lens 3, change direction through a spectroscope 4 again, arrive astigmatic lens 11 at last and image in four-quadrant photo detector 12, focusing parameter when the both direction of selecting astigmatic lens 11 can obtain certain focusing range and certain focusing accuracy.The lens 3 of focusing usefulness be fixed on can portable plate 7 by driven by motor on, biochip 8 is positioned on the sample retainer 9.
Goodbye Fig. 3.Four-quadrant photo detector 12 becomes the above-mentioned conversion of signals that receives four road electric signal amplifiers 13, wave filter 14 and A/D analog to digital converter 15 to enter digital signal processor 16 and computing machine 17, calculates and store the out of focus information of each analyzing spot.Then, mobile example in predetermined scanning area just can obtain the out of focus information of each point in the zone.When these points are all in the scope that can focus, target amount of bias according to digital signal processor 16 or computing machine 17 internal memories, send the automatic focusing order by digital signal processor 16 or computing machine 17, remove to drive actuating motor 21 by photoelectric isolation module 18, D/A digital to analog converter 19 and motor-driven power amplification circuit 20, drive focus adjusting mechanism and focus.
In Fig. 4, leading screw 22 is linked on the mounting plate 24 by support 23, and fixedly slide block 25 also is installed on the mounting plate 24, and camera lens 26 usefulness nuts are fixed on the removable bottom 27.Camera lens 26, removable bottom 27, movable guiding rail 28 and nut 29 connect together form can be up and down parts.Actuating motor 21 drives leading screw 22 by shaft joint 30 and rotates, thereby nut 29 is moved up and down along leading screw 22, drive camera lens 26 and move up and down along the optical axis direction, up to biochip 8 and on sample meet the requirements of the target focal plane position, finish the focusing operation.Photoelectric sensor 31 is fixed on fixedly on the slide block 25, plays the effect of positioning limit, avoids camera lens 26 and biochip 8 collisions, and the initialization of also can focus when hardware meets accident is located.
It can be with the mode detection of biological sample of automatic focusing, has that focusing range is big, advantage of high precision.
Claims (7)
1, a kind of automatic focusing device of laser cofocal scanner, contain optical scanning head, focusing control circuit and focus adjusting mechanism, it is characterized in that: described optical scanning head contains the input path that is made of laser instrument, pin hole, lens, object lens and sample successively, and the focusing that is made of above-mentioned sample and object lens, spectroscope, astigmatic lens and four-quadrant photo detector is with reflected light path and the detection reflected light path that is made of above-mentioned sample and object lens, dichroic beamsplitter, lens, pin hole and photomultiplier successively successively; Described focusing control circuit is to serve as the digital control circuit of main formation with digital signal processing circuit and actuating motor; Described focus adjusting mechanism is the leading screw-nut type mechanical focusing mechanism of joining with the actuating motor output shaft.
2, according to the automatic focusing device of the laser cofocal scanner of claim 1, it is characterized in that: the amplification that described focusing control circuit is linked to each other with the output terminal of above-mentioned four-quadrant photo detector by its input end successively, filtering and A/D change-over circuit, digital signal processor DSP, photoelectricity isolation, D/A conversion, amplifying circuit and actuating motor serial connection form.
3, according to the automatic focusing device of the laser cofocal scanner of claim 1 or 2, it is characterized in that: described digital signal processor DSP links to each other with computing machine.
4, according to the automatic focusing device of the laser cofocal scanner of claim 1, it is characterized in that: the focusing light path in the optical scanning head is reflective.
5, according to the automatic focusing device of the laser cofocal scanner of claim 1, it is characterized in that: the focusing light path in the optical scanning head is a transmission-type.
6, according to the automatic focusing device of the laser cofocus scanning of claim 1, it is characterized in that, described leading screw-nut type mechanical focusing mechanism is contained: motor base, support and the fixing mounting plate of slide block are housed, pass leading screw and nut that motor base, support are connected with the output shaft of motor by shaft joint, link to each other with nut and be connected one and can move and opposite side is equipped with the removable bottom that optical scanning head is a camera lens, be contained in the photoelectric sensor on the said fixing slide block with respect to the said fixing slide block.
7, according to the automatic focusing device of the laser cofocal scanner of claim 1, it is characterized in that: described sample is the biological sample on the biochip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021002622A CN1176367C (en) | 2002-01-11 | 2002-01-11 | Automatic focusing device of laser confocal scanner |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021002622A CN1176367C (en) | 2002-01-11 | 2002-01-11 | Automatic focusing device of laser confocal scanner |
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| CN1358999A true CN1358999A (en) | 2002-07-17 |
| CN1176367C CN1176367C (en) | 2004-11-17 |
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| CNB021002622A Expired - Fee Related CN1176367C (en) | 2002-01-11 | 2002-01-11 | Automatic focusing device of laser confocal scanner |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427923C (en) * | 2006-03-08 | 2008-10-22 | 东华大学 | Multifunctional chip detection device |
| CN101158644B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Method of Inducing Fluorescence in Rotary Multi-channel Based on Transmission Fiber |
| CN101158645B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Rotary Multi-channel Excitation Fluorescence Method Based on Input-Output Optical Fiber |
| CN103234629A (en) * | 2013-04-12 | 2013-08-07 | 中国科学院上海光学精密机械研究所 | Device for simultaneously measuring positions and angles of two optical beams on same incident plane |
| CN104976953A (en) * | 2015-06-26 | 2015-10-14 | 吉林大学 | Laser focusing deviation detection device |
| CN106526799A (en) * | 2016-11-28 | 2017-03-22 | 河北汉光重工有限责任公司 | High-stability high-energy laser receiving lens |
| CN107219207A (en) * | 2017-07-04 | 2017-09-29 | 福州大学 | A kind of automatic focusing method of CCD fluorescence biosensor chips scanner |
| CN107991769A (en) * | 2018-01-12 | 2018-05-04 | 凝辉(天津)科技有限责任公司 | Two-dimensional scan device |
| CN113495062A (en) * | 2020-04-07 | 2021-10-12 | 旭臻科技有限公司 | System for large-area microscopic photoluminescence scanning and mapping measurement |
| CN113960077A (en) * | 2021-11-18 | 2022-01-21 | 西凡仪器(深圳)有限公司 | An X-ray detection mechanism with confocal point of X-ray and visible light |
| CN114442257A (en) * | 2022-01-25 | 2022-05-06 | 之江实验室 | A large-scale high-precision beam focal plane tracking device |
| CN114994892A (en) * | 2022-05-09 | 2022-09-02 | 中国科学院化学研究所 | Laser confocal microscopy imaging system and method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101013136B (en) * | 2007-02-08 | 2011-07-20 | 北京工业大学 | Laser-induction fluorescence co-focusing scanning device and method |
-
2002
- 2002-01-11 CN CNB021002622A patent/CN1176367C/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427923C (en) * | 2006-03-08 | 2008-10-22 | 东华大学 | Multifunctional chip detection device |
| CN101158644B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Method of Inducing Fluorescence in Rotary Multi-channel Based on Transmission Fiber |
| CN101158645B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Rotary Multi-channel Excitation Fluorescence Method Based on Input-Output Optical Fiber |
| CN103234629A (en) * | 2013-04-12 | 2013-08-07 | 中国科学院上海光学精密机械研究所 | Device for simultaneously measuring positions and angles of two optical beams on same incident plane |
| CN103234629B (en) * | 2013-04-12 | 2015-05-13 | 中国科学院上海光学精密机械研究所 | Device for simultaneously measuring positions and angles of two optical beams on same incident plane |
| CN104976953A (en) * | 2015-06-26 | 2015-10-14 | 吉林大学 | Laser focusing deviation detection device |
| CN104976953B (en) * | 2015-06-26 | 2018-06-12 | 吉林大学 | Laser focuses on deviation detection device |
| CN106526799A (en) * | 2016-11-28 | 2017-03-22 | 河北汉光重工有限责任公司 | High-stability high-energy laser receiving lens |
| CN107219207B (en) * | 2017-07-04 | 2023-10-20 | 福州大学 | Automatic focusing method of CCD biochip fluorescence scanner |
| CN107219207A (en) * | 2017-07-04 | 2017-09-29 | 福州大学 | A kind of automatic focusing method of CCD fluorescence biosensor chips scanner |
| CN107991769A (en) * | 2018-01-12 | 2018-05-04 | 凝辉(天津)科技有限责任公司 | Two-dimensional scan device |
| CN107991769B (en) * | 2018-01-12 | 2020-07-10 | 凝辉(天津)科技有限责任公司 | Two-dimensional scanning device |
| CN113495062A (en) * | 2020-04-07 | 2021-10-12 | 旭臻科技有限公司 | System for large-area microscopic photoluminescence scanning and mapping measurement |
| CN113960077A (en) * | 2021-11-18 | 2022-01-21 | 西凡仪器(深圳)有限公司 | An X-ray detection mechanism with confocal point of X-ray and visible light |
| CN114442257A (en) * | 2022-01-25 | 2022-05-06 | 之江实验室 | A large-scale high-precision beam focal plane tracking device |
| CN114442257B (en) * | 2022-01-25 | 2024-04-16 | 之江实验室 | A large-range high-precision beam focal plane tracking device |
| CN114994892A (en) * | 2022-05-09 | 2022-09-02 | 中国科学院化学研究所 | Laser confocal microscopy imaging system and method |
Also Published As
| Publication number | Publication date |
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| CN1176367C (en) | 2004-11-17 |
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Owner name: QINGHUA UNIVERSITY; BOAO BIOLOGICAL CO., LTD. Free format text: FORMER NAME OR ADDRESS: QINGHUA UNIVERSITY; CAPITALBIO CORPORATION |
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| CP03 | Change of name, title or address |
Address after: 100084 mailbox 100084-82, Beijing City Co-patentee after: Capitalbio Corporation Patentee after: Tsinghua University Address before: 100084 mailbox 100084-82, Beijing City Co-patentee before: Boao Biochip Co., Ltd., Beijing Patentee before: Tsinghua University |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20041117 Termination date: 20200111 |