CN1350592A - 应用于原核生物和真核生物的基于核酸探针的诊断检验 - Google Patents

应用于原核生物和真核生物的基于核酸探针的诊断检验 Download PDF

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CN1350592A
CN1350592A CN00807503A CN00807503A CN1350592A CN 1350592 A CN1350592 A CN 1350592A CN 00807503 A CN00807503 A CN 00807503A CN 00807503 A CN00807503 A CN 00807503A CN 1350592 A CN1350592 A CN 1350592A
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托马斯·杰勒德·巴里
特伦斯·詹姆斯·史密斯
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Abstract

描述了能够作为核酸探针检验的靶点区来检测并鉴别原核生物和/或真核生物的ssrA基因或tmRNA即ssrA基因的RNA转录序列,或其片段。不同生物的tmRNA序列的核酸排列对比可用于鉴别序列内的同源区和非同源区,这些区域又可以用来设计属特异性和种特异性的寡聚核苷酸探针。这些新鉴定的同源和非同源区提供了在分子水平鉴别和检测生物体的基础。以此方式鉴别出的这种寡核苷酸探针可用于检测样品中的tmRNA,由此能够指示不同样品类型中存在的非病毒生物的生存能力。

Description

应用于原核生物和真核生物的基于核酸探针的诊断检验
技术领域
本发明涉及用于原核生物和/或真核生物的检测和鉴定的核酸检验中靶序列的鉴定。
技术背景
ssrA基因编码小而稳定的高拷贝数的转录RNA序列(tmRNA)，该基因在所有的细菌中都存在，并且近来证实这种基因还存在于叶绿体和硅藻体中。tmRNA具有tRNA和mRNA分子的双重功能，并且参与补救失去终止密码子的截短的mRNA，促进截短的多肽在蛋白酶进行降解之前完成通读翻译(Keiler，K.C.et al.(1996)，Science，271，990-993)。tmRNA的这种特殊功能使得研究者开始对其分子的二级结构与功能之间的关系进行分析。这些研究集中在来自不同微生物的tmRNA的二级结构的保守性以及这种结构保守性在进化上的意义和功能相关性。Matveeva等(Matveeva.O et al(1998)，Vol.16，No.13，1374-1375)用tmRNA作为含有二级结构的RNA的模型研究了寡核苷酸与RNA分子的结合。研究没有实现鉴定tmRNA上的位点以及设计用于治疗目的的反义寡核苷酸的目标。
现在用于细菌诊断的核酸靶点/探针的数量非常有限。因此，当前需要优先考虑鉴定出用于诊断目的的DNA和RNA靶点。用于发展探针的靶核苷酸序列例如可以是质粒，核糖体RNA基因，基因之间的序列，编码毒力因子的基因或随机基因组DNA片段。此外许多RNA分子已被人描述为可以作为基于RNA的检测的靶点如核糖体RNA和RNA酶P。
任何基于核酸探针的检验都需要对当前所研究的真核生物和原核生物的核酸序列的特征有清楚的了解。对原核生物或真核生物进行可靠的检测，所用核酸探针应该为高度特异性(即，和其它生物的核酸序列没有交叉反应)和高度敏感性(即，该生物的所有或多数株系都应该与探针反应并被检测到)。所以，优选的靶点序列应存在于目标生物体的所有的株系之中。该序列应具有显著的序列变异性以保证将目标物种与其它亲缘相近的物种能够区分开；但另一方面，应具有足够的序列保守性以保证能够检测出目标物种的所有株系。一般来说，对一个核酸序列的精确鉴定是进行核酸探针检验的基础，但精确鉴定是费时、困难和不确定的。到目前为止只有为数不多的几种方法能够用于发展核酸探针来进行较广范围的微生物检测。已经被证实可以作为开发探针中潜在可用的靶点的核酸序列包括如rRNA基因和RNA以及rRNA 16S/23S基因间序列。
多数核酸探针/靶点检验都集中于用细菌的高拷贝核糖体RNA(rRNA)和rRNA 16S/23S间隔区序列(欧洲专利No.0 395 292)来进行检测和鉴定。市场上已经有的很多成功的商品化细菌检测试剂盒是基于rRNA探针/靶点来检测多种微生物。这包括由Genprobe Inc.(SanDiego California)出售的基于16S rRNA的一系列商品化探针诊断试剂盒，以及由Innogenetics N.V.(Ghent，Belgium)出售的基于16S/23S间隔区序列的DNA探针。但是大多数这种诊断试剂盒都有其局限性，包括由于靶点序列的低拷贝数造成的敏感性缺乏以及很多情况下由亲缘相近的生物体之间的序列一致性引起的特异性缺乏。
能够直接应用于样品中指示其中存在的任何活的微生物的基于核酸的检测方法在食品、工业、环境和医学中的应用都有重大意义。
基于DNA的检测方法的一个缺点是他们不能区分活的和死的生物体。一些研究致力于用rRNA和mRNA作为细胞存活的指示剂(Sheridan，G.E.C.et al.(1998)Applied and Environmental Microbiology，64，1313-1318)。但是这些序列并不是合适的靶点，因为rRNA和mRNA在细菌死后在其细胞中还会存在48小时。
随着基于核酸的类似微阵列形式的能同时对多核酸靶点进行监测的技术的出现，现在需要鉴别和表征新核酸序列作为探针和/或靶点区域来检测和鉴别活的真核细胞和原核细胞。
发明公开
本发明提供ssrA基因或其片段作为对原核生物或真核生物的核酸探针检验中的靶点区域。
因此，本发明适用于除病毒外的所有生物体。
尚没有有关使用ssrA基因的区域作为靶点区来检测和鉴别原核生物和真核生物的其它核酸探针检验及其相应的优势的报道。
根据本发明的一个实施方案，ssrA基因分子中与从DNA分子5’末端起的高同源性区相对应的片段能够作为通用靶点区域。
在本发明的另一个实施方案中，ssrA基因分子中与从DNA分子3’末端起的高同源性区相对应的片段能够作为通用靶点区域。
在本发明的一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。
在本发明的另一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为用来产生物属特异性探针的靶点区。
如下文所描述，不同生物体的ssrA基因的核酸序列的对比表明这些分子的5’和3’区具有高度同源性，所以可以用为通用靶点序列区。在亲缘相近的生物体间，ssrA基因比其它细菌高拷贝数RNA有更显著程度的序列变异性。这些变异区可作为区分不同物种的核酸检验的理想靶点。
本发明还提供了tmRNA即ssrA基因的RNA转录序列或其片段用作为对原核生物或真核生物的核酸探针检验中的靶点区域。
根据本发明这一方面的一个实施方案，tmRNA分子中与从tmRNA5’末端起的一段高同源性区相对应的片段能够作为通用靶点区。
可替代地，tmRNA中与从tmRNA 3’末端起的一段高同源性区相对应的片段能够作为通用靶点区。
根据本发明这一方面的一个进一步实施方案，tmRNA分子中与低同源性区相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。
根据本发明的另一个进一步的实施方案，tmRNA分子中与低同源性区域相对应的片段能够作为用来产生属特异性探针的靶点区。
根据本发明的核酸探针(DNA或RNA)典型地含有来自于ssrA基因或tmRNA转录序列或它们的互补序列的至少10个核苷酸，这些探针被用于真核生物或原核生物的核酸探针杂交检验。探针与它的互补序列的杂交典型地通过在核酸探针上标记上放射性或非放射性(如比色的或荧光的)标记物来揭示。
在优选的实施方案中，所述的ssrA基因片段或tmRNA片段可作为用于扩增过程中所用的引物的基础。
与ssrA基因或tmRNA序列的5’或3’区相对应的通用寡核苷酸引物可按照本发明使用，以便从广泛的细菌谱中扩增ssrA基因或它所编码的tmRNA，有助于实现大范围生物体的同时扩增，同时能使特异性的核酸探针发生杂交并进行检测。
优选地，扩增过程的产物可以作为核酸探针检验中的靶点区。
更加优选地，tmRNA分子的cDNA转录序列被作为探针用于核酸杂交检验。
这种检测可以在体外完成或在原位完成。
这里所定义的靶点区能够作为区分活的和死的原核生物或真核生物的检测的基础。
与细菌死后存在于其细胞中的rRNA和mRNA不同，tmRNA在死亡的生物体中迅速降解。因此，tmRNA能够作为有用的靶点来区分死的和活的原核生物或真核生物，即，通过核酸探针直接与分离的细菌RNA杂交或者通过将RNA扩增并将扩增产物和核酸探针杂交来将死的和活的原核生物或真核生物进行区分。
优选地，靶点区以多种探针形式使用，以大范围地检测和/或鉴定原核生物或真核生物。
根据本发明的ssrA基因探针或tmRNA转录序列探针可以被连接到微阵列基因芯片系统上，以大范围地对原核生物或真核生物进行高通量的检测和鉴定。
根据本发明的靶点区能够作为试验中的探针来检测目标样品中的原核生物或真核生物。
上述目标样品包括生物样品例如呼吸道的组织样品，泌尿生殖道的组织样品或胃肠道的组织样品，或者体液如血液和血液组分，唾液或脑脊液。
根据本发明的检验还可以在其它样品中完成，如食品样品，环境样品包括空气、水、海洋和土壤样品，以及植物和动物来源的样品。
根据本发明，ssrA基因或tmRNA转录序列的片段可被用于获得原核生物或真核生物的DNA图谱的检验中，并因此能够区分同一物种的不同株系。
核酸序列的对比表明在每一物种之内都会在ssrA基因和tmRNA转录序列内发生序列变异。种内序列变异性能被用来区分同一物种的不同株系来研究流行病学、追踪例如爆发时的感染媒介，或者进行种群研究。
本发明的其它应用包括用ssrA基因，tmRNA转录序列或与其互补的DNA序列或其片段来设计用于治疗目的的试剂来对抗感染性的原核生物或真核生物。
这些试剂可包括反义mRNA或寡核苷酸、核酶和拮抗性多肽并且可以任何药学上适用的形式来使用。
因此，本发明通过使用tmRNA靶点能被用于只检测生物样品中存活的生物体。因此，在任何抗感染药物治疗期间和之后，tmRNA靶点都能够用于监测对于这些特异性感染媒介(如抗微生物和/或抗寄生虫治疗)的治疗效率。
在一个实施方案中，靶点区被用来监测药物对感染媒介的治疗效率。
在另一个实施方案中，靶点区被用来监测胃肠道中促进健康的微生物的存活率和水平。
本发明的这一方面涉及，例如，将包含于如酸乳酪或其它促进健康的食品中的促进健康(益生的)的微生物菌群引入肠道。能够持续监测这些微生物的存在及水平对于保证它们持续促进健康的功能是有必要的。tmRNA区能够作为检测存活的微生物，例如在粪便中的微生物的靶点，以监测促进健康的生物体的存在。
在一个进一步的实施方案中，检验被用于对原核生物或真核生物进行定量分析。
当使用探针杂交和/或体外扩增来检测样品中的微生物时，可以通过信号强度来测定所存在的微生物的数量。实时体外扩增的方法能被用来对样品中的生物体进行定量分析。因此，对样品中的生物体进行定量分析的能力对用于治疗目的的临床情况是非常重要的，如监测抗生素治疗或其它治疗手段或治疗效率。
本发明的另一个进一步的应用是使用ssrA基因序列的数据库来鉴定原核生物或真核生物。
本发明提供一系列用于ssrA基因和tmRNA序列5’和3’端的同源区和变异区的探针，这些探针来自于这些序列或这些序列的互补序列。下面为代表性的序列：伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCGAAGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCCTTTAGCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAAGTAGCTTAATTGTCGCGTGTCCGTCAGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA     SEQ ID NO：1伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCGAAGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCCUUUAGCCUUCGCUCCCCAGCUUCCGCUCGUAAGACGGGGAUAAAGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCAUUAAAUUAAAUCAAAGUAGCUUAAUUGUCGCGUGUCCGUCAGCAGGAUUAAGUGAAUUUAAAGACCGGACUAAACGUGUAGUGCUAACGGCAGAGGAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA     SEQ ID NO：2杀鲑气单胞菌(Aeromonas salmonicida)ssrA，内部部分AAGATTCACGAAACCCAAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCtTAATAACCTGCATAGAGCCCTTCTACCCTAGCTTGCCTGTGTCCTAGGGAATCGGAAGGTCATCCTTCACAGGATCGTGTGGAAGTCCTGCTCGGGGCGGAAGCATTAAAACCAATCGAGCTAGTCAATTCGTGGCGTGTCTCTCCGCAGCGGGTTGGCGAATGTAAAGAGTGACTAAGCATGTAGTACCGAGGATGTAGTAATTTTGGACGGGG  SEQ ID NO：3杀鲑气单胞菌(Aeromonas salmonicida)tmRNA，内部部分AAGAUUCACGAAACCCAAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCAUAGAGCCCUUCUACCCUAGCUUGCCUGUGUCCUAGGGAAUCGGAAGGUCAUCCUUCACAGGAUCGUGUGGAAGUCCUGCUCGGGGCGGAAGCAUUAAAACCAAUCGAGCUAGUCAAUUCGUGGCGUGUCUCUCCGCAGCGGGUUGGCGAAUGUAAAGAGUGACUAAGCAUGUAGUACCGAGGAUGUAGUAAUUUUGGACGGGG  SEQ ID NO：4富养产碱菌(Alcaligenes eutrophus)ssrATGGGCCGACCTGGTTTCGACGTGGTTACAAAGCAGTGAGGCATACCGAGGACCCGTCACCTCGTTAATCAATGGAATGCAATAACTGCTAACGACGAACGTTACGCACTCGCTTAATTGCGGCCGTCCTCGCACTGGCTCGCTGACGGGCTAGGGTCGCAAGACCACGCGAGGTATTTACGTCAGATAAGCTCCGGAAGGGTCACGAAGCCGGGGACGAAAACCTAGTGACTCGCCGTCGTAGAGCGTGTTCGTCCGATGCGCCGGTTAAATCAAATGACAGAACTAAGTATGTAGAACTCTCTGTGGAGGGCTTACGGACGCGGGTTCGATTCCCGCCGGCTCCACCA SEQ IDNO：5富养产碱菌(Alcaligenes eutrophus)tmRNAUGGGCCGACCUGGUUUCGACGUGGUUACAAAGCAGUGAGGCAUACCGAGGACCCGUCACCUCGUUAAUCAAUGGAAUGCAAUAACUGCUAACGACGAACGUUACGCACUCGCUUAAUUGCGGCCGUCCUCGCACUGGCUCGCUGACGGGCUAGGGUCGCAAGACCACGCGAGGUAUUUACGUCAGAUAAGCUCCGGAAGGGUCACGAAGCCGGGGACGAAAACCUAGUGACUCGCCGUCGUAGAGCGUGUUCGUCCGAUGCGCCGGUUAAAUCAAAUGACAGAACUAAGUAUGUAGAACUCUCUGUGGAGGGCUUACGGACGCGGGUUCGAUUCCCGCCGGCUCCACCA  SEQ ID NO：6产液菌(Aquifex aeolicus)ssrAGGGGGCGGAAAGGATTCGACGGGGACAGGCGGTCCCCGAGGAGCAGGCCGGGTGGCTCCCGTAACAGCCGCTAAAACAGCTCCCGAAGCTGAACTCGCTCTCGCTGCCTAATTAAACGGCAGCGCGTCCCCGGTAGGTTTGCGGGTGGCCTACCGGAGGGCGTCAGAGACACCCGCTCGGGCTACTCGGTCGCACGGGGCTGAGTAGCTGACACCTAACCCGTGCTACCCTCGGGGAGCTTGCCCGTGGGCGACCCGAGGGGAAATCCTGAACACGGGCTAAGCCTGTAGAGCCTCGGATGTGGCCGCCGTCCTCGGACGCGGGTTCGATTCCCGCCGCCTCCACCASEQ ID NO：7产液菌(Aquifex aeolicus)tmRNAGGGGGCGGAAAGGAUUCGACGGGGACAGGCGGUCCCCGAGGAGCAGGCCGGGUGGCUCCCGUAACAGCCGCUAAAACAGCUCCCGAAGCUGAACUCGCUCUCGCUGCCUAAUUAAACGGCAGCGCGUCCCCGGUAGGUUUGCGGGUGGCCUACCGGAGGGCGUCAGAGACACCCGCUCGGGCUACUCGGUCGCACGGGGCUGAGUAGCUGACACCUAACCCGUGCUACCCUCGGGGAGCUUGCCCGUGGGCGACCCGAGGGGAAAUCCUGAACACGGGCUAAGCCUGUAGAGCCUCGGAUGUGGCCGCCGUCCUCGGACGCGGGUUCGAUUCCCGCCGCCUCCACCA  SEQ ID NO：8巨大芽孢杆菌(Bacillus megaterium)ssrA，内部部分AGGGTAGTTCGAGCTTAGGTTGCGAGTCGAGGAGATGGCCTCGTTAAAACATCAACGCCAATAATAACTGGCAAATCTAACAATAACTTCGCTTTAGCTGCATAATAGTAGCTTAGCGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCACTTTAAGTGGGCTACGCCGGAGTTCGCCGTCTGAGGACGAAGGAAGAGAATAATCAGACTAGCGACTGGGACGCCTGTTGGTAGGCAGAACAGCTCGCGAATGATCAATATGCCAACTACACTCGTAGACGCTTAAGTGGCCATATTTCTGGACGTGG      SEQ ID NO：9巨大芽孢杆菌(Bacillus megaterium)tmRNA，内部部分AGGGUAGUUCGAGCUUAGGUUGCGAGUCGAGGAGAUGGCCUCGUUAAAACAUCAACGCCAAUAAUAACUGGCAAAUCUAACAAUAACUUCGCUUUAGCUGCAUAAUAGUAGCUUAGCGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCACUUUAAGUGGGCUACGCCGGAGUUCGCCGUCUGAGGACGAAGGAAGAGAAUAAUCAGACUAGCGACUGGGACGCCUGUUGGUAGGCAGAACAGCUCGCGAAUGAUCAAUAUGCCAACUACACUCGUAGACGCUUAAGUGGCCAUAUUUCUGGACGUGG  SEQ ID NO：10枯草芽孢杆菌(Bacillus subtilis)ssrAGGGGACGTTACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACATTGCCTATGTGTCTGTGAAGAGCACATCCAAGTAGGCTACGCTTGCGTTCCCGTCTGAGAACGTAAGAAGAGATGAACAGACTAGCTCTCGGAAGGCCCGCCCGCAGGCAAGAAGATGAGTGAAACCATAAATATGCAGGCTACGCTCGTAGACGCTTAAGTAATCGATGTTTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCA  SEQ ID NO：11枯草芽孢杆菌(Bacillus subtilis)tmRNAGGGGACGUUACGGAUUCGACAGGGAUGGAUCGAGCUUGAGCUGCGAGCCGAGAGGCGAUCUCGUAAACACGCACUUAAAUAUAACUGGCAAAACUAACAGUUUUAACCAAAACGUAGCAUUAGCUGCCUAAUAAGCGCAGCGAGCUCUUCCUGACAUUGCCUAUGUGUCUGUGAAGAGCACAUCCAAGUAGGCUACGCUUGCGUUCCCGUCUGAGAACGUAAGAAGAGAUGAACAGACUAGCUCUCGGAAGGCCCGCCCGCAGGCAAGAAGAUGAGUGAAACCAUAAAUAUGCAGGCUACGCUCGUAGACGCUUAAGUAAUCGAUGUUUCUGGACGUGGGUUCGACUCCCACCGUCUCCACCA  SEQ ID NO：12百日咳鲍特氏菌(Bordetella pertussis)ssrAGGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAGGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTCTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCAGGCGGGGGAAGGCAACTTCACAGGGGGCAACCCCGAACCGCAGCAGCGACATTCACAAGGAATCGGCCACCGCTGGGGTCACACGGCGTTGGTTTAAATTACGTGAATCGCCCTGGTCCGGCCCGTCGATCGGCTAAGTCCAGGGTTAAATCCAAATAGATCGACTAAGCATGTAGAACTGGTTGCGGAGGGCTTGCGGACGGGGGTTCAATTCCCCCCGGCTCCACCASEQ ID NO：13百日咳鲍特氏菌(Bordetella pertussis)tmRNAGGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAGGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUCUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCAGGCGGGGGAAGGCAACUUCACAGGGGGCAACCCCGAACCGCAGCAGCGACAUUCACAAGGAAUCGGCCACCGCUGGGGUCACACGGCGUUGGUUUAAAUUACGUGAAUCGCCCUGGUCCGGCCCGUCGAUCGGCUAAGUCCAGGGUUAAAUCCAAAUAGAUCGACUAAGCAUGUAGAACUGGUUGCGGAGGGCUUGCGGACGGGGGUUCAAUUCCCCCCGGCUCCACCASEQ ID NO：14布氏疏螺旋体(Borrelia burgdorferi)ssrAGGGGATGTTTTGGATTTGACTGAAAATGTTAATATTGTAAGTTGCAGGCAGAGGGAATCTCTTAAAACTTCTAAAATAAATGCAAAAAATAATAACTTTACAAGCTCAAATCTTGTAATGGCTGCTTAAGTTAGCAGAGGGTTTTGTTGAATTTGGCTTTGAGGTTCACTTATACTCTTTTCGACATCAAAGCTTGCTTAAAAATGTTTTCAAGTTGATTTTTAGGGACTTTTATACTTGAGAGCAATTTGGTGGTTTGCTAGTATTTCCAAACCATATTGCTTAATAAAATACTAGATAAGCTTGTAGAAGCTTATAGTATTATTTTTAGGACGCGGGTTCAATTCCCGCCATCTCCACCA  SEQ ID NO：15布氏疏螺旋体(Borrelia burgdorferi)tmRNAGGGGAUGUUUUGGAUUUGACUGAAAAUGUUAAUAUUGUAAGUUGCAGGCAGAGGGAAUCUCUUAAAACUUCUAAAAUAAAUGCAAAAAAUAAUAACUUUACAAGCUCAAAUCUUGUAAUGGCUGCUUAAGUUAGCAGAGGGUUUUGUUGAAUUUGGCUUUGAGGUUCACUUAUACUCUUUUCGACAUCAAAGCUUGCUUAAAAAUGUUUUCAAGUUGAUUUUUAGGGACUUUUAUACUUGAGAGCAAUUUGGUGGUUUGCUAGUAUUUCCAAACCAUAUUGCUUAAUAAAAUACUAGAUAAGCUUGUAGAAGCUUAUAGUAUUAUUUUUAGGACGCGGGUUCAAUUCCCGCCAUCUCCACCA    SEQ ID NO：16空肠弯曲杆菌(Campylobacter jejuni)ssrAGGGAGCGACTTGGCTTCGACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGACAGGGGTTCGATTCCCCTCGCTTCCACCASEQ ID NO：17空肠弯曲杆菌(Campylobacter jejuni)tmRNAGGGAGCGACUUGGCUUCGACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGGACAGGGGUUCGAUUCCCCUCGCUUCCACCA  SEQ ID NO：18沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTCGAAGATCTAAGAGTAGCTGCTTAATTAGCAAAGTTGTTACCTAAATACGGGTGACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACACCGTCATGTATCTGGTTAGAACTTAGGTCCTTTAATTCTCGAGGAAATGAGTTTGAAATTTAATGAGAGTCGTTAGTCTCTATAGGGGTTTCTAGCTGAGGAGACATAACGTATAGTACCTAGGAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGACTCTCTCCACCTCCACCA  SEQ ID NO：19沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUCGAAGAUCUAAGAGUAGCUGCUUAAUUAGCAAAGUUGUUACCUAAAUACGGGUGACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACACCGUCAUGUAUCUGGUUAGAACUUAGGUCCUUUAAUUCUCGAGGAAAUGAGUUUGAAAUUUAAUGAGAGUCGUUAGUCUCUAUAGGGGUUUCUAGCUGAGGAGACAUAACGUAUAGUACCUAGGAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGACUCUCUCCACCUCCACCASEQ ID NO：20沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTTAATGATCTAAGAGTTGCTGCTTAATTAGCAAAGTTGTTACCTAAGTACTGGTAACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACGCCGTCATTTATCTGGTTAGAATTAGGGCCTTTTAACTCTCAAGGGAACTAATTTGAATTTTAATGAGAGTCGTTGGTCTCTATAGAGGTTTCTAGCTGAGGAGATATAACGTAAAATATTCTAGAAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGAATCTCTCCACCTCCACCA   SEQ ID NO：21沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUUAAUGAUCUAAGAGUUGCUGCUUAAUUAGCAAAGUUGUUACCUAAGUACUGGUAACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACGCCGUCAUUUAUCUGGUUAGAAUUAGGGCCUUUUAACUCUCAAGGGAACUAAUUUGAAUUUUAAUGAGAGUCGUUGGUCUCUAUAGAGGUUUCUAGCUGAGGAGAUAUAACGUAAAAAUUCUAGAAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGAAUCUCUCCACCUCCACCASEQ ID NO：22微温杆状绿菌(Chlorobium tepidum)ssrAGGGGATGACAGGCTATCGACAGGATAGGTGTGAGATGTCGTTGCACTCCGAGTTTCAGCATGGACGGACTCGTTAAACAAGTCTATGTACCAATAGATGCAGACGATTATTCGTATGCAATGGCTGCCTGATTAGCACAAGTTAATTCAGAAGCCATCGTCCTGCGGTGAATGCGCTTACTCTGAAGCCGCCGGATGGCATAACCCGCGCTTGAGCCTACGGGTTCGCGCAAGTAAGCTCCGTACATTCATGCCCGAGGGGGTGTGCGGGTAACCAATCGGGATAAGGGGACGAACGCTGCTGGCGGTGTAATCGGACCACGAAAAACCAACCACCAGAGATGAGTGTGGTAACTGCATCGAGCAGTGTCCTGGACGCGGGTTCAAGTCCCGCCATCTCCACCA  SEQ ID NO：23微温杆状绿菌(Chlorobium tepidum)tmRNAGGGGAUGACAGGCUAUCGACAGGAUAGGUGUGAGAUGUCGUUGCACUCCGAGUUUCAGCAUGGACGGACUCGUUAAACAAGUCUAUGUACCAAUAGAUGCAGACGAUUAUUCGUAUGCAAUGGCUGCCUGAUUAGCACAAGUUAAUUCAGAAGCCAUCGUCCUGCGGUGAAUGCGCUUACUCUGAAGCCGCCGGAUGGCAUAACCCGCGCUUGAGCCUACGGGUUCGCGCAAGUAAGCUCCGUACAUUCAUGCCCGAGGGGGUGUGCGGGUAACCAAUCGGGAUAAGGGGACGAACGCUGCUGGCGGUGUAAUCGGACCACGAAAAACCAACCACCAGAGAUGAGUGUGGUAACUGCAUCGAGCAGUGUCCUGGACGCGGGUUCAAGUCCCGCCAUCUCCACCA  SEQ ID NO：24Cyanophora paradoxa(藻类)蓝色小体ssrAGGGGCTGTTTAGGTTTCGACGTTTTTTTCTAATTATGTTTGTTAAGCAAGTCGAGGATTTGTTCTATCTCGAAAATCAAGAACTCTCAAAATTTAAACGCAACTAATATTGTACGTTTTAACCGTAAAGCAGCTTTCGCTGTTTAATAATTACTTTTAATTTAAAAACCTAATTTTTTTAGGAATTTATTTATTTATTGTTTATCCTGCTTAATGAATTAAAAAAAGCTATACTTGTGAATAAACGCATAATTTAAAAAAACGGACGTGGGTTCAAATCCCACCAGCTCCACCA  SEQ ID NO：25Cyanophora paradoxa(藻类)蓝色小体tmRNAGGGGCUGUUUAGGUUUCGACGUUUUUUUCUAAUUAUGUUUGUUAAGCAAGUCGAGGAUUUGUUCUAUCUCGAAAAUCAAGAACUCUCAAAAUUUAAACGCAACUAAUAUUGUACGUUUUAACCGUAAAGCAGCUUUCGCUGUUUAAUAAUUACUUUUAAUUUAAAAACCUAAUUUUUUUAGGAAUUUAUUUAUUUAUUGUUUAUCCUGCUUAAUGAAUUAAAAAAAGCUAUACUUGUGAAUAAACGCAUAAUUUAAAAAAACGGACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO：26丙酮丁醇梭菌(Clostridium acetobutylicum)ssrA，3′部分AATCTGGCGTCGAGAGCGGGGAAACGAGCCTTACAAAGCTTTGAGTAAGGAACGGAATTTATGAAGCTACTGAAGTGAAAAGCTTGTTTGTAGGCGTTTCATGGAGGGAATGTTAAAATACAAACTGCACTCGGAGATGCTTAATGAAACCATTTTCGGACAGGGGTTCGATTCCCCTCGCCTCCACCA  SEQ ID NO：27丙酮丁醇梭菌(Clostridium acetobutylicum)tmRNA，3′部分AAUCUGGCGUCGAGAGCGGGGAAACGAGCCUUACAAAGCUUUGAGUAAGGAACGGAAUUUAUGAAGCUACUGAAGUGAAAAGCUUGUUUGUAGGCGUUUCAUGGAGGGAAUGUUAAAAUACAAACUGCACUCGGAGAUGCUUAAUGAAACCAUUUUCGGACAGGGGUUCGAUUCCCCUCGCCUCCACCA  SEQ ID NO：28放射异常球菌模式种(Deinococcus radiodurans)ssrAGGGGGTGACCCGGTTTCGACAGGGGAACTGAAGGTGATGTTGCGTGTCGAGGTGCCGTTGGCCTCGTAAACAAACGGCAAAGCCATTTAACTGGCAACCAGAACTACGCTCTCGCTGCTTAAGTGAGATGACGACCGTGCAGCCCGGCCTTTGGCGTCGCGGAAGTCACTAAAAAAGAAGGCTAGCCCAGGCGATTCTCCATAGCCGACGGCGAAACTTTATGGAGCTACGGCCTGCGAGAACCTGCCCACTGGTGAGCGCCGGCCCGACAATCAAACAGTGGGATACACACGTAGACGCACGCTGGACGGACCTTTGGACGGCGGTTCGACTCCGCCCACCTCCACCASEQ ID NO：29放射异常球菌模式种(Deinococcus radiodurans)tmRNAGGGGGUGACCCGGUUUCGACAGGGGAACUGAAGGUGAUGUUGCGUGUCGAGGUGCCGUUGGCCUCGUAAACAAACGGCAAAGCCAUUUAACUGGCAACCAGAACUACGCUCUCGCUGCUUAAGUGAGAUGACGACCGUGCAGCCCGGCCUUUGGCGUCGCGGAAGUCACUAAAAAAGAAGGCUAGCCCAGGCGAUUCUCCAUAGCCGACGGCGAAACUUUAUGGAGCUACGGCCUGCGAGAACCUGCCCACUGGUGAGCGCCGGCCCGACAAUCAAACAGUGGGAUACACACGUAGACGCACGCUGGACGGACCUUUGGACGGCGGUUCGACUCCGCCCACCUCCACCA  SEQ ID NO：30脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)ssrA，内部部分GGGACTGGAACCGTAGCGGCAGGTCGAGGCGCCGCTGGCCTCGTAAAAAGCGGCACAAAAGTAATTGCCAACAACGATTACGACTACGCTTACGCTGCCTAATAACAGCGAGGCAATGACCGTTTAACGGTCGCGCCGATCAGGGCCATGCCTGATAACCCTGATTGGCGACACTTATCAGGCTGGCGAAAACCGGCTCTCGCCGGGGTTTTTCGCGAGGAGTTTACCGGCGGGATTGCTGCGTTGTGCCTGGTCAGGGGCCAACAGCGCGGTGAAATACATACTTGACCTAAACCTGTAATGCTTCGTGTGGAATGTTCTCGGACGGGG   SEQ ID NO：31脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)tmRNA，内部部分GGGACUGGAACCGUAGCGGCAGGUCGAGGCGCCGCUGGCCUCGUAAAAAGCGGCACAAAAGUAAUUGCCAACAACGAUUACGACUACGCUUACGCUGCCUAAUAACAGCGAGGCAAUGACCGUUUAACGGUCGCGCCGAUCAGGGCCAUGCCUGAUAACCCUGAUUGGCGACACUUAUCAGGCUGGCGAAAACCGGCUCUCGCCGGGGUUUUUCGCGAGGAGUUUACCGGCGGGAUUGCUGCGUUGUGCCUGGUCAGGGGCCAACAGCGCGGUGAAAUACAUACUUGACCUAAACCUGUAAUGCUUCGUGUGGAAUGUUCUCGGACGGGG  SEQ ID NO：32节瘤腐蹄杆菌(Dichelobacter nodosus)ssrA，3′部分CTCGAGGTGCATGTCGAGAATGAGAGAATCTCGTTAAATACTTTCAAAACTTATAGTTGCAAACGACGACAACTACGCTTTAGCGGCTTAATTCCCGCTTTCGCTTACCTAGATTTGTCTGTGGGTTTACCGTAAGCGACATTAACACAGAATCGCTGGTTAACGCGTCCGCTGTTAATCGGTTAAATTAAGCGGAATCGCTTGTAAAATGCCTGAGCGTTGGCTGTTTATGAGTTAAACCTAATTAACTGCTCTAAACATGTAGTACCAAAAGTTAAGGATTCGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCA  SEQ ID NO：33节瘤腐蹄杆菌(Dichelobacter nodosus)tmRNA，3′部分CUCGAGGUGCAUGUCGAGAAUGAGAGAAUCUCGUUAAAUACUUUCAAAACUUAUAGUUGCAAACGACGACAACUACGCUUUAGCGGCUUAAUUCCCGCUUUCGCUUACCUAGAUUUGUCUGUGGGUUUACCGUAAGCGACAUUAACACAGAAUCGCUGGUUAACGCGUCCGCUGUUAAUCGGUUAAAUUAAGCGGAAUCGCUUGUAAAAUGCCUGAGCGUUGGCUGUUUAUGAGUUAAACCUAAUUAACUGCUCUAAACAUGUAGUACCAAAAGUUAAGGAUUCGCGGACGGGGGUUCAAAUCCCCCCGCCUCCACCA  SEQ ID NO：34肠球菌模式种(Enterococcus faecalis)ssrAGGGGGCGTTACGGATTCGACAGGCATAGTTGAGCTTGAATTGCGTTTCGTAGGTTACGGCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAATTCTTTCGCTTTAGCTGCCTAAAAACCAGCTAGCGAAGATCCTCCCGGCATCGCCCATGTGCTCGGGTCAGGGTCCTAATCGAAGTGGGATACGCTAAATTTTTCCGTCTGTAAAATTTAGAGGAGCTTACCAGACTAGCAATACAGAATGCCTGTCACTCGGCACGCTGTAAAGCGAACCTTTAAATGAGTGTCTATGAACGTAGAGATTTAAGTGGCAATATGTTTGGACGCGGGTTCGACTCCCGCCGTCTCCACCA  SEQ ID NO：35肠球菌模式种(Enterococcus faecalis)tmRNAGGGGGCGUUACGGAUUCGACAGGCAUAGUUGAGCUUGAAUUGCGUUUCGUAGGUUACGGCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAAUUCUUUCGCUUUAGCUGCCUAAAAACCAGCUAGCGAAGAUCCUCCCGGCAUCGCCCAUGUGCUCGGGUCAGGGUCCUAAUCGAAGUGGGAUACGCUAAAUUUUUCCGUCUGUAAAAUUUAGAGGAGCUUACCAGACUAGCAAUACAGAAUGCCUGUCACUCGGCACGCUGUAAAGCGAACCUUUAAAUGAGUGUCUAUGAACGUAGAGAUUUAAGUGGCAAUAUGUUUGGACGCGGGUUCGACUCCCGCCGUCUCCACCA  SEQ ID NO：36大肠杆菌(Escherichia coli)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA  SEQ ID NO：37大肠杆菌(Escherichia coli)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUUAAUCAGGCUAGUUUGUUAGUGGCGUGUCCGUCCGCAGCUGGCAAGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA  SEQ ID NO：38流感嗜血杆菌(Haemophilus influenzae)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCTCCAGCTTCCGCTCGTAAGACGGGGATAACGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCACTAAATTGAATCAAACTAGCTTAAGTTTAGCGTGTCTGTCCGCATGCTTAAGTGAAATTAAAGACGAGACTAAACGTGTAGTACTGAAGGTAGAGTAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA  SEQ ID NO：39流感嗜血杆菌(Haemophilus influenzae)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCUCCAGCUUCCGCUCGUAAGACGGGGAUAACGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCACUAAAUUGAAUCAAACUAGCUUAAGUUUAGCGUGUCUGUCCGCAUGCUUAAGUGAAAUUAAAGACGAGACUAAACGUGUAGUACUGAAGGUAGAGUAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA  SEQ ID NO：40幽门螺杆菌(Helicobacter pylori)(ATCC 43504)ssrA，内部部分AGATTTCTTGTCGCGCAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTTAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAACTAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGG  SEQ ID NO：41幽门螺杆菌(Helicobacter pylori)(ATCC 43504)tmRNA，内部部分AGAUUUCUUGUCGCGCAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUUAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAACUAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGSEQ ID NO：42幽门螺杆菌(Helicobacter pylori)(菌株26695)ssrAGGGGCTGACTTGGATTTCGACAGATTTCTTGTCGCACAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTCAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAAACCAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGGGTTCGATTCCCCACAGCTCCACCA  SEQ ID NO：43幽门螺杆菌(Helicobacter pylori)(菌株26695)tmRNAGGGGCUGACUUGGAUUUCGACAGAUUUCUUGUCGCACAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUCAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAAACCAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGGUUCGAUUCCCCACAGCUCCACCASEQ ID NO：44产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)ssrA，内部部分GGGATTCGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTAAGAGCCCTCTCTCCCTAGCTTCCGCTCCTAAGACGGGGAATAAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTAATCAGGCTAGTTTGTCAGTGGCGTGTCCGTCCGCAGCTGGCCAGCGAATGTAAAGACTGGACTAAGCATGTAGTGCCGAGGATGTAGGAATTTC   SEQ ID NO：45产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)tmRNA，内部部分GGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUAAGAGCCCUCUCUCCCUAGCUUCCGCUCCUAAGACGGGGAAUAAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUAAUCAGGCUAGUUUGUCAGUGGCGUGUCCGUCCGCAGCUGGCCAGCGAAUGUAAAGACUGGACUAAGCAUGUAGUGCCGAGGAUGUAGGAAUUUC  SEQ ID NO：46乳杆菌模式种(Lactobacillus lactis)(NCTC 662)ssrA，内部部分AAGCACAGTTCGAGCTTGAATTGCGTTTCGTAGGTTACGTCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAACTCTTACGCTTTAGCTGCCTAAAAACAGTTAGCGTAGATCCTCTCGGCATCGCCCATGTGCTCGAGTAAGGGTCTCAAATTTAGTGGGATACGTTAAACTTTTCCGTCTGTAAAGTTTAAAAGAGATCATCAGACTAGCGATACAGAATGCCTGTCACTCGGCAAGCTGTAAAGCGAAACCTCAAATGAGTTGACTATGAACGTAGATTTTTAAGTGTCGATGTGTTT  SEQ ID NO：47乳杆菌模式种(Lactobacillus lactis)(NCTC 662)tmRNA，内部部分AAGCACAGUUCGAGCUUGAAUUGCGUUUCGUAGGUUACGUCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAACUCUUACGCUUUAGCUGCCUAAAAACAGUUAGCGUAGAUCCUCUCGGCAUCGCCCAUGUGCUCGAGUAAGGGUCUCAAAUUUAGUGGGAUACGUUAAACUUUUCCGUCUGUAAAGUUUAAAAGAGAUCAUCAGACUAGCGAUACAGAAUGCCUGUCACUCGGCAAGCUGUAAAGCGAAACCUCAAAUGAGUUGACUAUGAACGUAGAUUUUUAAGUGUCGAUGUGUUU  SEQ ID NO：48嗜肺军团菌模式种(Legionella pneumophila)ssrA，内部部分GTGGGTTGCAAAACCGGAAGTGCATGCCGAGAAGGAGATCTCTCGTAAATAAGACTCAATTAAATATAAATGCAAACGATGAAAACTTTGCTGGTGGGGAAGCTATCGCTGCCTAATAAGCACTTTAGTTAAACCATCACTGTGTACTGGCCAATAAACCCAGTATCCCGTTCGACCGAGCCCGCTTATCGGTATCGAATCAACGGTCATAAGAGATAAGCTAGCGTCCTAATCTATCCCGGGTTATGGCGCGAAACTCAGGGAATCGCTGTGTATCATCCTGCCCGTCGGAGGAGCCACAGTTAAATTCAAAAGACAAGGCTATGCATGTAGAGCTAAAGGCAGAGGACTTGCGGACGCGG  SEQ ID NO：49嗜肺军团菌模式种(Legionella pneumophila)tmRNA，内部部分GUGGGUUGCAAAACCGGAAGUGCAUGCCGAGAAGGAGAUCUCUCGUAAAUAAGACUCAAUUAAAUAUAAAUGCAAACGAUGAAAACUUUGCUGGUGGGGAAGCUAUCGCUGCCUAAUAAGCACUUUAGUUAAACCAUCACUGUGUACUGGCCAAUAAACCCAGUAUCCCGUUCGACCGAGCCCGCUUAUCGGUAUCGAAUCAACGGUCAUAAGAGAUAAGCUAGCGUCCUAAUCUAUCCCGGGUUAUGGCGCGAAACUCAGGGAAUCGCUGUGUAUCAUCCUGCCCGUCGGAGGAGCCACAGUUAAAUUCAAAAGACAAGGCUAUGCAUGUAGAGCUAAAGGCAGAGGACUUGCGGACGCGG  SEQ ID NO：50格氏李斯特氏菌(Listeria grayi)ssrA，内部部分ACAGGGATAGGTCGAGCTTGAGTTGCGAGCCGGGGGGATCGGCCCGTCATCAACGTCAAAGCCAATAATAACTGGCAAACAAAACAACAATTTAGCTTTCGCTGCCTAATAGCAGTCTGAATAGCTGATCCTCCGTGCATCACCCATGTGCTACGGTAAGGGTCTCACTTTTAAGTGGGTTACGCTGGCTTATCTCCGTCTGGGGCAAACGAGAAGAGCATAATCAGACTAGCTAGATAGAGCCCTGACGCCGGGCAGACATCTATGCGAAATCCAAATACGGCAACTACGCTCGTAGATGCTCAAGTGCCGATATTTCTGG  SEQ ID NO：51格氏李斯特氏菌(Listeria grayi)tmRNA，内部部分ACAGGGAUAGGUCGAGCUUGAGUUGCGAGCCGGGGGGAUCGGCCCGUCAUCAACGUCAAAGCCAAUAAUAACUGGCAAACAAAACAACAAUUUAGCUUUCGCUGCCUAAUAGCAGUCUGAAUAGCUGAUCCUCCGUGCAUCACCCAUGUGCUACGGUAAGGGUCUCACUUUUAAGUGGGUUACGCUGGCUUAUCUCCGUCUGGGGCAAACGAGAAGAGCAUAAUCAGACUAGCUAGAUAGAGCCCUGACGCCGGGCAGACAUCUAUGCGAAAUCCAAAUACGGCAACUACGCUCGUAGAUGCUCAAGUGCCGAUAUUUCUGG  SEQ ID NO：52无害李斯特氏菌(Listeria innocua)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG  SEQ ID NO：53无害李斯特氏菌(Listeria innocua)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG  SEQ ID NO：54单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 7973)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG  SEQ ID NO：55单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC7973)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG  SEQ ID NO：56单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)ssrA，内部部分CAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTT  SEQ ID NO：57单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)tmRNA，内部部分CAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUSEQ ID NO：58墨氏李斯特氏菌(Listeria murrayi)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATGAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG  SEQ ID NO：59墨氏李斯特氏菌(Listeria murrayi)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUGAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG  SEQ ID NO：60威氏李斯特氏菌(Listeria welshimeri)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTGGCTAATCTCCGTCTGAGGTTAGTTGGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG  SEQ ID NO：61威氏李斯特氏菌(Listeria welshimeri)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUGGCUAAUCUCCGUCUGAGGUUAGUUGGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG  SEQ ID NO：62除烃海杆菌(Marinobacter hydrocarbonoclasticus)ssrA，内部部分GCCGGTGACGAACCCTTGGGTGCATGCCGAGATGGCAGCGAATCTCGTAAATCCAAAGCTGCAACGTAATAGTCGCAAACGACGAAAACTACGCACTGGCGGCGTAAGCCGTTCCAGTCGTCCTGGCTGAGGCGCCTATAACTCAGTAGCAACATCCCAGGACGTCATCGCTTATAGGCTGCTCCGTTCACCAGAGCTCACTGGTGTTCGGCTAAGATTAAAGAGCTCGCCTCTTGCACCCTGACCTTCGGGTCGCTTGAGGTTAAATCAATAGAAGGACACTAAGCATGTAGACCTCAAGGCCTAGTGCTGGCGGACGCGG  SEQ ID NO：63除烃海杆菌(Marinobacter hydrocarbonoclasticus)tmRNA，内部部分GCCGGUGACGAACCCUUGGGUGCAUGCCGAGAUGGCAGCGAAUCUCGUAAAUCCAAAGCUGCAACGUAAUAGUCGCAAACGACGAAAACUACGCACUGGCGGCGUAAGCCGUUCCAGUCGUCCUGGCUGAGGCGCCUAUAACUCAGUAGCAACAUCCCAGGACGUCAUCGCUUAUAGGCUGCUCCGUUCACCAGAGCUCACUGGUGUUCGGCUAAGAUUAAAGAGCUCGCCUCUUGCACCCUGACCUUCGGGUCGCUUGAGGUUAAAUCAAUAGAAGGACACUAAGCAUGUAGACCUCAAGGCCUAGUGCUGGCGGACGCGG  SEQ ID NO：64鸟分枝杆菌(Mycobacterium avium)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA  SEQ ID NO：65鸟分枝杆菌(Mycobacterium avium)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO：66牛分枝杆菌(Mycobacterium bovis)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGG  SEQ ID NO：67牛分枝杆菌(Mycobacterium bovis)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGG  SEQ ID NO：68麻风分枝杆菌(Mycobacterium leprae)ssrAGGGGCTGAAAGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAGCAATATAAGCGCCGATTCATATCAGCGCGACTATGCTCTCGCTGCCTAAGCGATGGCTAGTCTGTCAGACCGGGAACGCCCTCGTCCCGGAGCCTGGCATCAGCTAGAGGGATCTACCGATGGGTTCGGTCGCGGGACTCGTCGGGACACCAACCGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGATCAGGAGATCCGAGTAGAGGCATAGCGAACTACGCACGGAGAAGCCTTGAGGGAAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA  SEQ ID NO：69麻风分枝杆菌(Mycobacterium leprae)tmRNAGGGGCUGAAAGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAGCAAUAUAAGCGCCGAUUCAUAUCAGCGCGACUAUGCUCUCGCUGCCUAAGCGAUGGCUAGUCUGUCAGACCGGGAACGCCCUCGUCCCGGAGCCUGGCAUCAGCUAGAGGGAUCUACCGAUGGGUUCGGUCGCGGGACUCGUCGGGACACCAACCGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGAUCAGGAGAUCCGAGUAGAGGCAUAGCGAACUACGCACGGAGAAGCCUUGAGGGAAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA  SEQ ID NO：70鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA  SEQ ID NO：71鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO：72结核分枝杆菌(Mycobacterium tuberculosis)ssrAGGGGCTGAACGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA  SEQ ID NO：73结核分枝杆菌(Mycobacterium tuberculosis)tmRNAGGGGCUGAACGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA  SEQ ID NO：74山羊枝原体(Mycoplasma capricolum)ssrAGGGGATGTCATGGATTTGACAGGATATCTTTAGTACATATAAGCAGTAGTGTTGTAGACTATAAATACTACTAGGTTTAAAAAAACGCAAATAAAAACGAAGAAACTTTTGAAATGCCAGCATTTATGATGAATAATGCATCAGCTGGAGCAAACTTTATGTTTGCTTAATAACTACTAGTTTAGTTATAGTATTTCACGAATTATAGATATTTTAAGCTTTATTTATAACCGTATTACCCAAGCTTAATAGAATATATGATTGCAATAAATATATTTGAAATCTAATTGCAAATGATATTTAACCTTTAGTTAATTTTAGTTAAATATTTTAATTAGAAAATTAACTAAACTGTAGAAAGTATGTATTAATATATCTTGGACGCGAGTTCGATTCTCGCCATCTCCACCA  SEQ ID NO：75山羊枝原体(Mycoplasma capricolum)tmRNAGGGGAUGUCAUGGAUUUGACAGGAUAUCUUUAGUACAUAUAAGCAGUAGUGUUGUAGACUAUAAAUACUACUAGGUUUAAAAAAACGCAAAUAAAAACGAAGAAACUUUUGAAAUGCCAGCAUUUAUGAUGAAUAAUGCAUCAGCUGGAGCAAACUUUAUGUUUGCUUAAUAACUACUAGUUUAGUUAUAGUAUUUCACGAAUUAUAGAUAUUUUAAGCUUUAUUUAUAACCGUAUUACCCAAGCUUAAUAGAAUAUAUGAUUGCAAUAAAUAUAUUUGAAAUCUAAUUGCAAAUGAUAUUUAACCUUUAGUUAAUUUUAGUUAAAUAUUUUAAUUAGAAAAUUAACUAAACUGUAGAAAGUAUGUAUUAAUAUAUCUUGGACGCGAGUUCGAUUCUCGCCAUCUCCACCA  SEQ ID NO：76生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)ssrAGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGAACCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA  SEQ ID NO：77生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)tmRNAGGGGAUGUUUUGGGUUUGACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAACCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA   SEQ ID NO：78生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分ACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCSEQ ID NO：79生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分ACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAUCCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUC  SEQ ID NO：80嗜肺枝原体(Mycoplasma pneumophila)ssrAGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA  SEQ ID NO：81嗜肺枝原体(Mycoplasma pneumophila)tmRNAGGGGAUGUAGAGGUUUUGACAUAAUGUUGAAAGGAAAACAGUUGCAGUGGGGUAUGCCCCUUACAGCUCUAGGUAUAAUAACCGACAAAAAUAACGACGAAGUUUUGGUAGAUCCAAUGUUGAUCGCUAACCAACAAGCAAGUAUCAACUACGCUUUCGCUUAGAACAUACUAAAGCUACACGAAUUGAAUCGCCAUAGUUUGGUUCGUGUCACAGUUUAUGGCUCGGGGUUAACUGGUUCAACUUAAUCCUUAAAUUAUGAACUUAUCGUUUACUUGUUUGUCUUAUGAUCUAAAGUAAGCGAGACAUUAAAACAUAAGACUAAACUGUAGAAGCUGUUUUACCAAUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA  SEQ ID NO：82淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)ssrA，内部部分GGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGG  SEQ ID NO：83淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)tmRNA，内部部分GGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGG  SEQ ID NO：84淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGTGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA  SEQ ID NO：85淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGUGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA  SEQ ID NO：86脑膜炎奈瑟氏球菌(Neisseria meningitidis)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCTGCCGGGTTATTTGGCAGGAAATGAGATTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA  SEQ ID NO：87脑膜炎奈瑟氏球菌(Neisseria meningitidis)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCUGCCGGGUUAUUUGGCAGGAAAUGAGAUUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA  SEQ ID NO：88Nostoc muscorum PCC7120 ssrAGGGTCCGTCGGTTTCGACAGGTTGGCGAACGCTACTCTGTGATTCAGGTCGAGAGTGAGTCTCCTCTGCAAATCAAGGCTCAAAACAAAAGTAAATGCGAATAACATCGTTAAATTTGCTCGTAAGGACGCTCTAGTAGCTGCCTAAATAGCCTCTTTCAGGTTCGAGCGTCTTCGGTTTGACTCCGTTAAGGACTGAAGACCAACCCCCAACGGATGCTCTAGCAATGTTCTCTGGTTGGCTTGCTAGCTAAGATTTAATCAGAGCATCCTACGTTCGGGATAATGAACGATTCCCGCCTTGAGGGTCAGAAAGGCTAAACCTGTGAATGAGCGGGGGGTCAATACCCAATTTGGACAGCAGTTCGACTCTGCTCGATCCACCA  SEQ ID NO：89Nostoc muscorum PCC7120 tmRNAGGGUCCGUCGGUUUCGACAGGUUGGCGAACGCUACUCUGUGAUUCAGGUCGAGAGUGAGUCUCCUCUGCAAAUCAAGGCUCAAAACAAAAGUAAAUGCGAAUAACAUCGUUAAAUUUGCUCGUAAGGACGCUCUAGUAGCUGCCUAAAUAGCCUCUUUCAGGUUCGAGCGUCUUCGGUUUGACUCCGUUAAGGACUGAAGACCAACCCCCAACGGAUGCUCUAGCAAUGUUCUCUGGUUGGCUUGCUAGCUAAGAUUUAAUCAGAGCAUCCUACGUUCGGGAUAAUGAACGAUUCCCGCCUUGAGGGUCAGAAAGGCUAAACCUGUGAAUGAGCGGGGGGUCAAUACCCAAUUUGGACAGCAGUUCGACUCUGCUCGAUCCACCASEQ ID NO：90Odontella sinensis(硅藻属)叶绿体ssrAGGGGCTGACTTGGTTTCGACATTTAAAAATTGTTACAGTATGATGCAGGTCGAAGTTTCTAATCTTCGTAAAAAAAGAGAAATTTATAATAAATGCTAATAATTTAATTTCTTCTGTGTTTAAAAGTTTATCAACTAAGCAAAATAGTTTAAATTTAAGTTTTGCTGTTTAAGTTTTATGCACATTTAATGATCTAGTAAATAACTTTGTTCGCTATAATTTATATTTATAACTAGACTTTTGTCTTTTTTATAGTTTAGAATAACTTTATCATTTCAAACCTCGTTCCATCTAGTTGAACTAAACCTGTGAACGAATACTATAATAAAATTTTTAGATGGACGTGGGTTCGACTCCCATCAGCTCCACCA  SEQ ID NO：91Odontella sinensis(硅藻属)叶绿体tmRNAGGGGCUGACUUGGUUUCGACAUUUAAAAAUUGUUACAGUAUGAUGCAGGUCGAAGUUUCUAAUCUUCGUAAAAAAAGAGAAAUUUAUAAUAAAUGCUAAUAAUUUAAUUUCUUCUGUGUUUAAAAGUUUAUCAACUAAGCAAAAUAGUUUAAAUUUAAGUUUUGCUGUUUAAGUUUUAUGCACAUUUAAUGAUCUAGUAAAUAACUUUGUUCGCUAUAAUUUAUAUUUAUAACUAGACUUUUGUCUUUUUUAUAGUUUAGAAUAACUUUAUCAUUUCAAACCUCGUUCCAUCUAGUUGAACUAAACCUGUGAACGAAUACUAUAAUAAAAUUUUUAGAUGGACGUGGGUUCGACUCCCAUCAGCUCCACCASEQ ID NO：92卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体ssrAGGGGCTGCAAGGTTTCTACATTGTGAAAAAACAAATATATGAAAGTAAAACGAGCTCATTATTAGAGCTTTTAGTTAAATAAATGCAGAAAATAATATTATTGCTTTTTCTCGAAAATTAGCTGTTGCATAAATAGTCTCAATTTTTGTAATTCGAAGTGATAGACTCTTATACACTACGAATATTCTGTTAGAGTTGCTCTTAATAAAAGAAAAGTAAAAAAATACAAATTCTTATGTTTTTTACCTGAATTGATTCAATTTAAGGTTAGTATTTTTTGATTTTTACAATGGACGTGGGTTCAAGTCCCACCAGCTCCACCA  SEQ ID NO：93卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体tmRNAGGGGCUGCAAGGUUUCUACAUUGUGAAAAAACAAAUAUAUGAAAGUAAAACGAGCUCAUUAUUAGAGCUUUUAGUUAAAUAAAUGCAGAAAAUAAUAUUAUUGCUUUUUCUCGAAAAUUAGCUGUUGCAUAAAUAGUCUCAAUUUUUGUAAUUCGAAGUGAUAGACUCUUAUACACUACGAAUAUUCUGUUAGAGUUGCUCUUAAUAAAAGAAAAGUAAAAAAAUACAAAUUCUUAUGUUUUUUACCUGAAUUGAUUCAAUUUAAGGUUAGUAUUUUUUGAUUUUUACAAUGGACGUGGGUUCAAGUCCCACCAGCUCCACCA  SEQ ID NO：94牙龈卟啉单胞菌(Porphyromonas gingivalis)ssrAGGGGCTGACCGGCTTTGACAGCGTGATGAAGCGGTATGTAAGCATGTAGTGCGTGGGTGGCTTGCACTATAATCTCAGACATCAAAAGTTTAATTGGCGAAAATAACTACGCTCTCGCTGCGTAATCGAAGAATAGTAGATTAGACGCTTCATCGCCGCCAAAGTGGCAGCGACGAGACATCGCCCGAGCAGCTTTTTCCCGAAGTAGCTCGATGGTGCGGTGCTGACAAATCGGGAACCGCTACAGGATGCTTCCTGCCTGTGGTCAGATCGAACGGAAGATAAGGATCGTGCATTGGGTCGTTTCAGCCTCCGCTCGCTCACGAAAATTCCAACTGAAACTAAACATGTAGAAAGCATATTGATTCCATGTTTGGACGAGGGTTCAATTCCCTCCAGCTCCACCA  SEQ ID NO：95牙龈卟啉单胞菌(Porphyromonas gingivalis)tmRNAGGGGCUGACCGGCUUUGACAGCGUGAUGAAGCGGUAUGUAAGCAUGUAGUGCGUGGGUGGCUUGCACUAUAAUCUCAGACAUCAAAAGUUUAAUUGGCGAAAAUAACUACGCUCUCGCUGCGUAAUCGAAGAAUAGUAGAUUAGACGCUUCAUCGCCGCCAAAGUGGCAGCGACGAGACAUCGCCCGAGCAGCUUUUUCCCGAAGUAGCUCGAUGGUGCGGUGCUGACAAAUCGGGAACCGCUACAGGAUGCUUCCUGCCUGUGGUCAGAUCGAACGGAAGAUAAGGAUCGUGCAUUGGGUCGUUUCAGCCUCCGCUCGCUCACGAAAAUUCCAACUGAAACUAAACAUGUAGAAAGCAUAUUGAUUCCAUGUUUGGACGAGGGUUCAAUUCCCUCCAGCUCCACCA  SEQ ID NO：96雷氏变性菌(Proteus rettgeri)ssrA(NCTC 10975)，内部部分GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCTTGCCTGGGGTTGAAGCGTTAAACTTAATCAGGATAGTTTGTTGGTGGCGTGTCTGTCCGCAGCTGGCAAATGAATTCAAAGACTAGACTAAGCATGTAGTACCGAGGATGTAGAAATTTCSEQ ID NO：97雷氏变性菌(Proteus rettgeri)ssrA tmRNA(NCTC 10975)，内部部分GGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCUUGCCUGGGGUUGAAGCGUUAAACUUAAUCAGGAUAGUUUGUUGGUGGCGUGUCUGUCCGCAGCUGGCAAAUGAAUUCAAAGACUAGACUAAGCAUGUAGUACCGAGGAUGUAGAAAUUUC  SEQ ID NO：98游海假交替单胞菌(Pseudoalteromonas haloplanktoni)ssrA，内部部分GGAATTCAAGAAGCCCGAGGTGCATGTCGAGGTGCGGTTTGCCTCGTAAAAAAGCCGCAATTTAAAGTAATCGCAAACGACGATAACTACTCTCTAGCAGCTTAGGCTGGCTAGCGCTCCTTCCATGTATTCTTGTGGACTGGATTTTGGAGTGTCACCCTAACACCTGATCGCGACGGAAACCCTGGCCGGGGTTGAAGCGTTAAAACTAAGCGGCCTCGCCTTTATCTACCGTGTTTGTCCGGGATTTAAAGGTTAATTAAATGACAATACTAAACATGTAGTACCGACGGTCGAGGCTTTTCGGACGGGG  SEQ ID NO：99游海假交替单胞菌(Pseudoalteromonas haloplanktoni)tmRNA，内部部分GGAAUUCAAGAAGCCCGAGGUGCAUGUCGAGGUGCGGUUUGCCUCGUAAAAAAGCCGCAAUUUAAAGUAAUCGCAAACGACGAUAACUACUCUCUAGCAGCUUAGGCUGGCUAGCGCUCCUUCCAUGUAUUCUUGUGGACUGGAUUUUGGAGUGUCACCCUAACACCUGAUCGCGACGGAAACCCUGGCCGGGGUUGAAGCGUUAAAACUAAGCGGCCUCGCCUUUAUCUACCGUGUUUGUCCGGGAUUUAAAGGUUAAUUAAAUGACAAUACUAAACAUGUAGUACCGACGGUCGAGGCUUUUCGGACGGGG  SEQ ID NO：100铜绿假单胞菌(Pseudomonas aeruginosa)ssrAGGGGCCGATTAGGATTCGACGCCGGTAACAAAAGTTGAGGGGCATGCCGAGTTGGTAGCAGAACTCGTAAATTCGCTGCTGCAAACTTATAGTTGCCAACGACGACAACTACGCTCTAGCTGCTTAATGCGGCTAGCAGTCGCTAGGGGATGCCTGTAAACCCGAAACGACTGTCAGATAGAACAGGATCGCCGCCAAGTTCGCTGTAGACGTAACGGCTAAAACTCATACAGCTCGCTCCAAGCACCCTGCCACTCGGGCGGCGCGGAGTTAACTCAGTAGAGCTGGCTAAGCATGTAAAACCGATAGCGGAAAGCTGGCGGACGGGGGTTCAAATCCCCCCGGTTCCACCASEQ ID NO：101铜绿假单胞菌(Pseudomonas aeruginosa)tmRNAGGGGCCGAUUAGGAUUCGACGCCGGUAACAAAAGUUGAGGGGCAUGCCGAGUUGGUAGCAGAACUCGUAAAUUCGCUGCUGCAAACUUAUAGUUGCCAACGACGACAACUACGCUCUAGCUGCUUAAUGCGGCUAGCAGUCGCUAGGGGAUGCCUGUAAACCCGAAACGACUGUCAGAUAGAACAGGAUCGCCGCCAAGUUCGCUGUAGACGUAACGGCUAAAACUCAUACAGCUCGCUCCAAGCACCCUGCCACUCGGGCGGCGCGGAGUUAACUCAGUAGAGCUGGCUAAGCAUGUAAAACCGAUAGCGGAAAGCUGGCGGACGGGGGUUCAAAUCCCCCCGGUUCCACCA  SEQ ID NO：102鼠伤寒沙门氏菌(Salmonella typhimurium)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGCGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA  SEQ ID NO：103鼠伤寒沙门氏菌(Salmonella typhimurium)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGCGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA  SEQ ID NO：104希瓦氏菌模式种(Shewanella putrefaciens)ssrAGGGGGCGATTCTGGATTCGACAGGATTCACGAAACCCTGGGAGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAGTTATAGTTGCAAACGACGATAACTACGCTCTAGCCGCTTAATGCCGCTAGCCATCTACCACACGCTTTGCACATGGGCAGTGGATTTGATGGTCATCTCACATCGTGCTAGCGAGGGAACCCTGTCTGGGGGTGAACCGCGAAACAGTACCGGACTCACCGTGTGGGATCCTGTCTTTCGGAGTTCAAACGGTTAAACAATAGAAAGACTAAGCATGTAGCGCCTTGGATGTAGGTTTTCTGGACGCGGGTTCAAGTCCCGCCGCCTCCACCASEQ ID NO：105希瓦氏菌模式种(Shewanella putrefaciens)tmRNAGGGGGCGAUUCUGGAUUCGACAGGAUUCACGAAACCCUGGGAGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAGUUAUAGUUGCAAACGACGAUAACUACGCUCUAGCCGCUUAAUGCCGCUAGCCAUCUACCACACGCUUUGCACAUGGGCAGUGGAUUUGAUGGUCAUCUCACAUCGUGCUAGCGAGGGAACCCUGUCUGGGGGUGAACCGCGAAACAGUACCGGACUCACCGUGUGGGAUCCUGUCUUUCGGAGUUCAAACGGUUAAACAAUAGAAAGACUAAGCAUGUAGCGCCUUGGAUGUAGGUUUUCUGGACGCGGGUUCAAGUCCCGCCGCCUCCACCA  SEQ ID NO：106金黄色葡萄球菌(Staphylococcus aureus)ssrAGGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTCGGAGGGTTGTCTTCGTCATCAACACACACAGTTTATAATAACTGGCAAATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCATTTCCTATGTGCTGTTAACGCGATTCAACCTTAATAGGATATGCTAAACACTGCCGTTTGAAGTCTGTTTAGAAGAAACTTAATCAAACTAGCATCATGTTGGTTGTTTATCACTTTTCATGATGCGAAACCTATCGATAAACTACACACGTAGAAAGATGTGTATCAGGACCTTTGGACGCGGGTTCAAATCCCGCCGTCTCCACCA  SEQ ID NO：107金黄色葡萄球菌(Staphylococcus aureus)tmRNAGGGGACGUUCAUGGAUUCGACAGGGGUCCCCCGAGCUCAUUAAGCGUGUCGGAGGGUUGUCUUCGUCAUCAACACACACAGUUUAUAAUAACUGGCAAAUCAAACAAUAAUUUCGCAGUAGCUGCCUAAUCGCACUCUGCAUCGCCUAACAGCAUUUCCUAUGUGCUGUUAACGCGAUUCAACCUUAAUAGGAUAUGCUAAACACUGCCGUUUGAAGUCUGUUUAGAAGAAACUUAAUCAAACUAGCAUCAUGUUGGUUGUUUAUCACUUUUCAUGAUGCGAAACCUAUCGAUAAACUACACACGUAGAAAGAUGUGUAUCAGGACCUUUGGACGCGGGUUCAAAUCCCGCCGUCUCCACCA  SEQ ID NO：108戈氏链球菌(Streptococcus gordonii)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATAATACTTCTTACGCTTTAGCTGCCTAAAAACCAGCGGGCGTGACCCGATTCGGATTGCTTGTGTCTGATGACAGGTCTTATTATTAGCAAGCTACGGTAGAATCTTGTCTAGTGATTTTACAAGAGATTGATAGACTCGCTTGATTTGGGCTTGAGTTATGTGTCAAAATCAAGTTAAAACAATACATAGCCTATGGTTGTAGACAAATGTGTTGGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCA SEQ ID NO：109戈氏链球菌(Streptococcus gordonii)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUAAUACUUCUUACGCUUUAGCUGCCUAAAAACCAGCGGGCGUGACCCGAUUCGGAUUGCUUGUGUCUGAUGACAGGUCUUAUUAUUAGCAAGCUACGGUAGAAUCUUGUCUAGUGAUUUUACAAGAGAUUGAUAGACUCGCUUGAUUUGGGCUUGAGUUAUGUGUCAAAAUCAAGUUAAAACAAUACAUAGCCUAUGGUUGUAGACAAAUGUGUUGGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA  SEQ ID NO：110变异链球菌(Streptococcus mutans)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGACCTATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCAGTAGCTGCCTAAAAACCAGCCTGTGTGATCAATAACAAATTGCTTGTGTTTGTTGATTGGTCTTATTGTTAACAAGCTACGTTAGAACTGAGTCAGGCTGTTCTAAAAGAGTTCTACTGACTCGCATCGTTAGAGTTTGAGTTATGTATTGTAACGGTGTTAAATAAACACATAACCTATAGTTGTAGACAAATGGGTTAGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO：111变异链球菌(Streptococcus mutans)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGACCUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAGUAGCUGCCUAAAAACCAGCCUGUGUGAUCAAUAACAAAUUGCUUGUGUUUGUUGAUUGGUCUUAUUGUUAACAAGCUACGUUAGAACUGAGUCAGGCUGUUCUAAAAGAGUUCUACUGACUCGCAUCGUUAGAGUUUGAGUUAUGUAUUGUAACGGUGUUAAAUAAACACAUAACCUAUAGUUGUAGACAAAUGGGUUAGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA  SEQ ID NO：112肺炎链球菌(Streptococcus pneumoniae)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCGTGTGGCGACGTAAACGCTCAGTTAAATATAACTGCAAAAAATAACACTTCTTACGCTCTAGCTGCCTAAAAACCAGCAGGCGTGACCCGATTTGGATTGCTCGTGTTCAATGACAGGTCTTATTATTAGCGAGATACGATTAAGCCTTGTCTAGCGGTTTGATAAGAGATTGATAGACTCGCAGTTTCTAGACTTGAGTTATGTGTCGAGGGGCTGTTAAAATAATACATAACCTATGGTTGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO：113肺炎链球菌(Streptococcus pneumoniae)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCGUGUGGCGACGUAAACGCUCAGUUAAAUAUAACUGCAAAAAAUAACACUUCUUACGCUCUAGCUGCCUAAAAACCAGCAGGCGUGACCCGAUUUGGAUUGCUCGUGUUCAAUGACAGGUCUUAUUAUUAGCGAGAUACGAUUAAGCCUUGUCUAGCGGUUUGAUAAGAGAUUGAUAGACUCGCAGUUUCUAGACUUGAGUUAUGUGUCGAGGGGCUGUUAAAAUAAUACAUAACCUAUGGUUGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA  SEQ ID NO：114化脓链球菌(Streptococcus pyogenes)ssrAGGGGTTGTTACGGATTCGACAGGCATTATGAGGCATGTTTTGCGTCCCATCGGCAGATGTAAATTGCCAGTTAAATATAACTGCAAAAAATACAAACTCTTACGCTTTAGCTGCCTAAAAACCAGCTAGCGTGACTTCTACAAGATTGCTTGTGTCCTGTTAGAAGTCTCAAAATAGCAAGCTACGGTTACGAAATTGTCTAGTTTCGTGACAAGAGATTGATAGACTCGCAAACTAATGGCTTGAGTTATGTGTCTTTAGTTTGTTAAATGAAGACATAACCTATGGACGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCAGCTCCACCASEQ ID NO：115化脓链球菌(Streptococcus pyogenes)tmRNAGGGGUUGUUACGGAUUCGACAGGCAUUAUGAGGCAUGUUUUGCGUCCCAUCGGCAGAUGUAAAUUGCCAGUUAAAUAUAACUGCAAAAAAUACAAACUCUUACGCUUUAGCUGCCUAAAAACCAGCUAGCGUGACUUCUACAAGAUUGCUUGUGUCCUGUUAGAAGUCUCAAAAUAGCAAGCUACGGUUACGAAAUUGUCUAGUUUCGUGACAAGAGAUUGAUAGACUCGCAAACUAAUGGCUUGAGUUAUGUGUCUUUAGUUUGUUAAAUGAAGACAUAACCUAUGGACGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCAGCUCCACCA  SEQ ID NO：116Synechococcus sp.PCC6301 ssrAGGGGCTGTAATGGTTTCGACGTGTTGGTGAATCCTTCACCGTGATTCAGGCCGAGAGGGAGTCCACTCTCGTAAATCCAGGCTCAACCAAAAGTAACTGCGAACAACATCGTTCCTTTCGCTCGTAAGGCTGCTCCTGTAGCTGCTTAAACGCCACAAACTTTCTGGCTCGAGCGTCTAGTCGTAGACTCCGTTAATACGCCTAGACTTAAACCCCCAACGGATGCTCGAGTGGCGGCCTCAGGTCCGTCCTCTCGCTAAGCAAAAACCTGAGCATCCCGCCAACGGGGATAATCGTTGGCTCCCGCACAGTGGGTCAACCGTGCTAAGCCTGTGAACGAGCGGAAAGTTACTAGTCAATGCGGACAGCGGTTCGATTCCGCTCAGCTCCACCASEQ ID NO：117Synechococcus sp.PCC6301 tmRNAGGGGCUGUAAUGGUUUCGACGUGUUGGUGAAUCCUUCACCGUGAUUCAGGCCGAGAGGGAGUCCACUCUCGUAAAUCCAGGCUCAACCAAAAGUAACUGCGAACAACAUCGUUCCUUUCGCUCGUAAGGCUGCUCCUGUAGCUGCUUAAACGCCACAAACUUUCUGGCUCGAGCGUCUAGUCGUAGACUCCGUUAAUACGCCUAGACUUAAACCCCCAACGGAUGCUCGAGUGGCGGCCUCAGGUCCGUCCUCUCGCUAAGCAAAAACCUGAGCAUCCCGCCAACGGGGAUAAUCGUUGGCUCCCGCACAGUGGGUCAACCGUGCUAAGCCUGUGAACGAGCGGAAAGUUACUAGUCAAUGCGGACAGCGGUUCGAUUCCGCUCAGCUCCACCA  SEQ ID NO：118Synechocystis sp.PCC6803 ssrAGGGGCCGCAATGGTTTCGACAGGTTGGCGAAAGCTTGCCCGTGATACAGGTCGAGAGTGAGTCTCCTCTCGCAAATCAAAGGCTCAAAAAAAAGTAACTGCGAATAACATCGTCAGCTTCAAACGGGTAGCCATAGCAGCCTAGTCTGTAAAAGCTACATTTTCTTGTCAAAGACCGTTTACTTCTTTTCTGACTCCGTTAAGGATTAGAGGTTAACCCCAACGGATGCTTTGTTTGGCTCTTCTCTAGTTAGCTAAACAATCAAGACTCAGACTAGAGCATCCCACCATCAGGGATAATCGATGGTCCCCGTCCTAGGGCTAGAAGGACTAAACCTGTGAATGAGCGGAAAGTTAATACCCAGTTTGGACAGCAGTTCAATTCTGCTCGGCTCCACCASEQ ID NO：119Synechocystis sp.PCC6803 tmRNAGGGGCCGCAAUGGUUUCGACAGGUUGGCGAAAGCUUGCCCGUGAUACAGGUCGAGAGUGAGUCUCCUCUCGCAAAUCAAAGGCUCAAAAAAAAGUAACUGCGAAUAACAUCGUCAGCUUCAAACGGGUAGCCAUAGCAGCCUAGUCUGUAAAAGCUACAUUUUCUUGUCAAAGACCGUUUACUUCUUUUCUGACUCCGUUAAGGAUUAGAGGUUAACCCCAACGGAUGCUUUGUUUGGCUCUUCUCUAGUUAGCUAAACAAUCAAGACUCAGACUAGAGCAUCCCACCAUCAGGGAUAAUCGAUGGUCCCCGUCCUAGGGCUAGAAGGACUAAACCUGUGAAUGAGCGGAAAGUUAAUACCCAGUUUGGACAGCAGUUCAAUUCUGCUCGGCUCCACCA  SEQ ID NO：120海栖热袍菌(Thermotoga maritima)ssrAGGGGGCGAACGGGTTCGACGGGGATGGAGTCCCCTGGGAAGCGAGCCGAGGTCCCCACCTCCTCGTAAAAAAGGTGGGACAAAGAATAAGTGCCAACGAACCTGTTGCTGTTGCCGCTTAATAGATAAGCGGCCGTCCTCTCCGAAGTTGGCTGGGCTTCGGAAGAGGGCGTGAGAGATCCAGCCTACCGATTCAGCTTCGCCTTCCGGCCTGAATCGGGAAAACTCAGGAAGGCTGTGGGAGAGGACACCCTGCCCGTGGGAGGTCCCTCCCGAGAGCGAAAACACGGGCTGCGCTCGGAGAAGCCCAGGGGCCTCCATCTTCGGACGGGGGTTCGAATCCCCCCGCCTCCACCA  SEQ ID NO：121海栖热袍菌(Thermotoga maritima)tmRNAGGGGGCGAACGGGUUCGACGGGGAUGGAGUCCCCUGGGAAGCGAGCCGAGGUCCCCACCUCCUCGUAAAAAAGGUGGGACAAAGAAUAAGUGCCAACGAACCUGUUGCUGUUGCCGCUUAAUAGAUAAGCGGCCGUCCUCUCCGAAGUUGGCUGGGCUUCGGAAGAGGGCGUGAGAGAUCCAGCCUACCGAUUCAGCUUCGCCUUCCGGCCUGAAUCGGGAAAACUCAGGAAGGCUGUGGGAGAGGACACCCUGCCCGUGGGAGGUCCCUCCCGAGAGCGAAAACACGGGCUGCGCUCGGAGAAGCCCAGGGGCCUCCAUCUUCGGACGGGGGUUCGAAUCCCCCCGCCUCCACCA  SEQ ID NO：122嗜热栖热菌(Thermus thermophilus)ssrAGGGGGTGAAACGGTCTCGACGGGGGTCGCCGAGGGCGTGGCTGCGCGCCGAGGTGCGGGTGGCCTCGTAAAAACCCGCAACGGCATAACTGCCAACACCAACTACGCTCTCGCGGCTTAATGACCGCGACCTCGCCCGGTAGCCCTGCCGGGGGCTCACCGGAAGCGGGGACACAAACCCGGCTAGCCCGGGGCCACGCCCTCTAACCCCGGGCGAAGCTTGAAGGGGGCTCGCTCCTGGCCGCCCGTCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACTACGCGCGTAGAGGCCCGCCGTAGAGACCTTCGGACGGGGGTTCGACTCCCCCCACCTCCACCASEQ ID NO：123嗜热栖热菌(Thermus thermophilus)tmRNAGGGGGUGAAACGGUCUCGACGGGGGUCGCCGAGGGCGUGGCUGCGCGCCGAGGUGCGGGUGGCCUCGUAAAAACCCGCAACGGCAUAACUGCCAACACCAACUACGCUCUCGCGGCUUAAUGACCGCGACCUCGCCCGGUAGCCCUGCCGGGGGCUCACCGGAAGCGGGGACACAAACCCGGCUAGCCCGGGGCCACGCCCUCUAACCCCGGGCGAAGCUUGAAGGGGGCUCGCUCCUGGCCGCCCGUCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACUACGCGCGUAGAGGCCCGCCGUAGAGACCUUCGGACGGGGGUUCGACUCCCCCCACCUCCACCA  SEQ ID NO：124苍白密螺旋体(Treponema pallidum)ssrAGGGGATGACTAGGTTTCGACTAGGGATGTGGGGTGTTGCGCTGCAGGTGGAGTGTCGATCTCCTGATTCGGCGCCTTTATAACTGCCAATTCTGACAGTTTCGACTACGCGCTCGCCGCGTAATCGCGGGCCTGTGTTTGCGCTGCTCTGAGCGAACATATCGGCCCGACGCCAAACGGAGCTTGCTCTTACGTTGTGCACGGCGGACGTAGGGGGACTTTTGTCTGTGCTAAGACTCTGGCGCGTGCGGTGCAGGCCTAGCAGAGTCCGACAAACGCAGTACGCACCGCTAAACCTGTAGGCGCGCAGCACTCGCTCTTTAGGACGGGGGTTCGATTCCCCCCATCTCCACCASEQ ID NO：125苍白密螺旋体(Treponema pallidum)tmRNAGGGGAUGACUAGGUUUCGACUAGGGAUGUGGGGUGUUGCGCUGCAGGUGGAGUGUCGAUCUCCUGAUUCGGCGCCUUUAUAACUGCCAAUUCUGACAGUUUCGACUACGCGCUCGCCGCGUAAUCGCGGGCCUGUGUUUGCGCUGCUCUGAGCGAACAUAUCGGCCCGACGCCAAACGGAGCUUGCUCUUACGUUGUGCACGGCGGACGUAGGGGGACUUUUGUCUGUGCUAAGACUCUGGCGCGUGCGGUGCAGGCCUAGCAGAGUCCGACAAACGCAGUACGCACCGCUAAACCUGUAGGCGCGCAGCACUCGCUCUUUAGGACGGGGGUUCGAUUCCCCCCAUCUCCACCA  SEQ ID NO：126霍乱弧菌(Vibrio cholerae)ssrAGGGGCTGATTCAGGATTCGACGGGAATTTTGCAGTCTGAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCTTAATACCCTGCTCAGAGCCCTTCCTCCCTAGCTTCCGCTTGTAAGACGGGGAAATCAGGAAGGTCAAACCAAATCAAGCTGGCGTGGATTCCCCCACCTGAGGGATGAAGCGCGAGATCTAATTCAGGTTAGCCATTCGTTAGCGTGTCGGTTCGCAGGCGGTGGTGAAATTAAAGATCGACTAAGCATGTAGTACCAAAGATGAATGGTTTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA  SEQ ID NO：127霍乱弧菌(Vibrio cholerae)tmRNAGGGGCUGAUUCAGGAUUCGACGGGAAUUUUGCAGUCUGAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUACCCUGCUCAGAGCCCUUCCUCCCUAGCUUCCGCUUGUAAGACGGGGAAAUCAGGAAGGUCAAACCAAAUCAAGCUGGCGUGGAUUCCCCCACCUGAGGGAUGAAGCGCGAGAUCUAAUUCAGGUUAGCCAUUCGUUAGCGUGUCGGUUCGCAGGCGGUGGUGAAAUUAAAGAUCGACUAAGCAUGUAGUACCAAAGAUGAAUGGUUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA  SEQ ID NO：128鼠疫耶尔森氏菌(Yersinia pestis)ssrAGGGGCTGATTCTGGATTCGACGGGATTCGCGAAACCCAAGGTGCATGCCGAGGTGCGGTGGCCTCGTAAAAAACCGCAAAAAAAATAGTTGCAAACGACGAAAACTACGCACTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTGCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCTAAAAGAGCTCGTGTGGAAACCTTGCCTGGGGTGGAAGCATTAAAACTAATCAGGATAGTTTGTCAGTAGCGTGTCCATCCGCAGCTGGCCGGCGAATGTAATGATTGGACTAAGCATGTAGTGCCGACGGTGTAGTAATTTCGGACGGGGGTTCAAATCCCCCCAGCTCCACCA  SEQ ID NO：129鼠疫耶尔森氏菌(Yersinia pestis)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGUGCGGUGGCCUCGUAAAAAACCGCAAAAAAAAUAGUUGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUGCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCUAAAAGAGCUCGUGUGGAAACCUUGCCUGGGGUGGAAGCAUUAAAACUAAUCAGGAUAGUUUGUCAGUAGCGUGUCCAUCCGCAGCUGGCCGGCGAAUGUAAUGAUUGGACUAAGCAUGUAGUGCCGACGGUGUAGUAAUUUCGGACGGGGGUUCAAAUCCCCCCAGCUCCACCA  SEQ ID NO：130胎儿弯曲杆菌(Campylobacter fetus)ssrA，内部部分AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATCTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO：131胎儿弯曲杆菌(Campylobacter fetus)tmRNA，内部部分AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUCUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG  SEQ ID NO：132大肠弯曲杆菌(Campylobacter coli)(BM2509)ssrA，内部部分AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGACAAAGCGTAAAAAGTCCAAATTAAAATTAAACGCAAATAACGTTAAATTTGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCCGAAACTCAAGTGATGCTATCTAGCTTGAATTTTGGTCATCTTTGATAGTGTAGATTGAAAATTGACAACTTTTAATCGAAGTTAAAGTCTTAGTCTAGCTTGAAATTTTGGAAGGTGAGTTTAGCCAGATGAAGTTTTCACCTTTGCTAAACATGTAGAAGTCTTTGTGGGGTTATTTTTGGSEQ ID NO：133大肠弯曲杆菌(Campylobacter coli)(BM2509)tmRNA，内部部分AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGACAAAGCGUAAAAAGUCCAAAUUAAAAUUAAACGCAAAUAACGUUAAAUUUGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCCGAAACUCAAGUGAUGCUAUCUAGCUUGAAUUUUGGUCAUCUUUGAUAGUGUAGAUUGAAAAUUGACAACUUUUAAUCGAAGUUAAAGUCUUAGUCUAGCUUGAAAUUUUGGAAGGUGAGUUUAGCCAGAUGAAGUUUUCACCUUUGCUAAACAUGUAGAAGUCUUUGUGGGGUUAUUUUUGG  SEQ ID NO：134鸡弯曲杆菌分离物(Camplyobacter chicken isolate)ssrA，内部部分ACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAGGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO：135鸡弯曲杆菌分离物(Camplyobacter chicken isolate)tmRNA，内部部分ACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAGGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG  SEQ ID NO：136产气荚膜梭菌(Clostridium perfringens)ssrA，内部部分ACGGGGGTAGGATGGGTTTGATAAGCGAGTCGAGGGAAGCATGGTGCCTCGATAATAAAGTATGCATTAAAGATAAACGCACGAGATAATTTTGCATTAGCAGCTTAAGTTAGCGCTGCTCATCCTTCCTCAATTGCCCACGGTTGAGAGTAAGGGTGTCATTTAAAAGTGGGGAACCGAGCCTAGCAAAGCTTTGAGCTAGGAACGGAATTTATGAAGCTTACCAAAGAGGAAGTTTGTCTGTGGACGTTCTCTGAGGGAATTTTAAAACACAAGACTACACTCGTAGAAAGTCTTACTGGTCTGCTTTCGG  SEQ ID NO：137产气荚膜梭菌(Clostridium perfringens)tmRNA，内部部分ACGGGGGUAGGAUGGGUUUGAUAAGCGAGUCGAGGGAAGCAUGGUGCCUCGAUAAUAAAGUAUGCAUUAAAGAUAAACGCACGAGAUAAUUUUGCAUUAGCAGCUUAAGUUAGCGCUGCUCAUCCUUCCUCAAUUGCCCACGGUUGAGAGUAAGGGUGUCAUUUAAAAGUGGGGAACCGAGCCUAGCAAAGCUUUGAGCUAGGAACGGAAUUUAUGAAGCUUACCAAAGAGGAAGUUUGUCUGUGGACGUUCUCUGAGGGAAUUUUAAAACACAAGACUACACUCGUAGAAAGUCUUACUGGUCUGCUUUCGG  SEQ ID NO：138杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)ssrA，内部部分ACGGGATTAGCGAAGTCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAACAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCCCTAGCTTTCGCTCGTAAGACGGGGAGCACGCGGAGTCAAACCAAAACGAGATCGTGTGGACGCTTCCGCTTGTAGATGAAACACTAAATTGAATCAAGCTAGTTTATTTCTTGCGTGTCTGTCCGCTGGAGATAAGCGAAATTAAAGACCAGACTAAACGTGTAGTACTGAAGATAGAGTAATTTCGGACCCGGGTTCGACTC  SEQ ID NO：139杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)tmRNA，内部部分ACGGGAUUAGCGAAGUCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCCCUAGCUUUCGCUCGUAAGACGGGGAGCACGCGGAGUCAAACCAAAACGAGAUCGUGUGGACGCUUCCGCUUGUAGAUGAAACACUAAAUUGAAUCAAGCUAGUUUAUUUCUUGCGUGUCUGUCCGCUGGAGAUAAGCGAAAUUAAAGACCAGACUAAACGUGUAGUACUGAAGAUAGAGUAAUUUCGGACCCGGGUUCGACUCSEQ ID NO：140无害李斯特氏菌(Listeria innocua)(食物分离物#1)ssrA，内部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAACCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAA  SEQ ID NO：141无害李斯特氏菌(Listeria innocua)(食物分离物 #1)tmRNA，内部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAACCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAA  SEQ ID NO：142无害李斯特氏菌(Listeria innocua)(食物分离物 #2)ssrA，内部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA  SEQ ID NO：143无害李斯特氏菌(Listeria innocua)(食物分离物#2)tmRNA，内部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA  SEQ ID NO：144无害李斯特氏菌(Listeria innocua)(食物分离物#3)ssrA，内部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGAATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCGGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA  SEQ ID NO：145无害李斯特氏菌(Listeria innocua)(食物分离物 #3)tmRNA，内部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGAAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCGGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA  SEQ ID NO：146无害李斯特氏菌(Listeria innocua)(ATCC 12210)ssrA，内部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACTGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA  SEQ ID NO：147无害李斯特氏菌(Listeria innocua)(ATCC 12210)tmRNA，内部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACUGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA  SEQ ID NO：148伊氏李斯特氏菌(Listeria ivarnovii)(NCTC 11846)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG  SEQ ID NO：149伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG  SEQ ID NO：150斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG  SEQ ID NO：151斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG  SEQ ID NO：152肠炎沙门氏菌(Salmonella enteritidis)ssrA，内部部分ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGG  SEQ ID NO：153肠炎沙门氏菌(Salmonella enteritidis)tmRNA，内部部分ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG  SEQ ID NO：154表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，内部部分ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO：155表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，内部部分ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUAUAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG  SEQ ID NO：156无乳链球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，内部部分ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGGSEQ ID NO：157无乳链球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，内部部分ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG  SEQ ID NO：158支气管炎博德特氏菌(Bordetella bronchiseptica)ssrAGGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAAGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTTTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCACGCGGGGGAASEQ ID NO：159支气管炎博德特氏菌(Bordetella bronchiseptica)tmRNAGGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAAGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUUUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCACGCGGGGGAASEQ ID NO：160肺炎衣原体(Chlamydia pneumoniae)(CWL029)，ssrAGGGGGTGTATAGGTTTCGACTTGAAAATGAAGTGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCAACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATTAGCTTGTTTGACTCAGTAGAGGAAAGACTAGCTGCTTAATTAGCAAAAGTTGTTAGCTAGATAATCTCTAGGTAACCCGGTATCTGCGAGCTCCACCAGAGGCTTGCAAAATACCGTCATTTATCTGGTTGGAACTTACTTTCTCTAATTCTCAAGGAAGTTCGTTCGAGATTTTTGAGAGTCATTGGCTGCTATAGAGGCTTCTAGCTAAGGGAGTCCAATGTAAACAATTCTAGAAGATAAGCATGTAGAGGTTAGCAGGGAGTTTGTCAAGGACGAGAGTTCGAGTCTCTCCACCTCCACCA  SEQ ID NO：161肺炎衣原体(Chlamydia pneumoniae)(CWL029)tmRNAGGGGGUGUAUAGGUUUCGACUUGAAAAUGAAGUGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCAACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUUAGCUUGUUUGACUCAGUAGAGGAAAGACUAGCUGCUUAAUUAGCAAAAGUUGUUAGCUAGAUAAUCUCUAGGUAACCCGGUAUCUGCGAGCUCCACCAGAGGCUUGCAAAAUACCGUCAUUUAUCUGGUUGGAACUUACUUUCUCUAAUUCUCAAGGAAGUUCGUUCGAGAUUUUUGAGAGUCAUUGGCUGCUAUAGAGGCUUCUAGCUAAGGGAGUCCAAUGUAAACAAUUCUAGAAGAUAAGCAUGUAGAGGUUAGCAGGGAGUUUGUCAAGGACGAGAGUUCGAGUCUCUCCACCUCCACCASEQ ID NO：162土拉热弗朗西丝氏菌(Francisella tularensis)ssrAGGGGGCGAATATGGTTTCGACATGAATGTCAAAATCTAAGGTGCATGCCGAGGAAGTACCGTAACCTCGTTAATAACAGTACAAATGCCAATAATAACTGGCAACAAAAAAGCAAACCGCGTAGCGGCTAACGACAGCAACTTTGCTGCTGTTGCTAAAGCTGCCTAGTCTAGCTTAATAATCTAGATGCGCACGGATATGATAGTCTTTCTTATGACACTATCTATACATCCGTTCATATTCCGCATAAGACGGTCTTTGCTTTTTGTCTGGGAGTTAAGGCTGTATTTAACAGACTCGCTAACTATTACCCTGGCTAATTGGGGAATAGTCAAGCTAAACTCAAATAGATTAGCCTAAGCATGTAGATCCAAAGATCTAGAGTTTGTGGACGCGGGTTCAAATCCCGCCGCCTCCACCA  SEQ ID NO：163土拉热弗朗西丝氏菌(Francisella tularensis)tmRNAGGGGGCGAAUAUGGUUUCGACAUGAAUGUCAAAAUCUAAGGUGCAUGCCGAGGAAGUACCGUAACCUCGUUAAUAACAGUACAAAUGCCAAUAAUAACUGGCAACAAAAAAGCAAACCGCGUAGCGGCUAACGACAGCAACUUUGCUGCUGUUGCUAAAGCUGCCUAGUCUAGCUUAAUAAUCUAGAUGCGCACGGAUAUGAUAGUCUUUCUUAUGACACUAUCUAUACAUCCGUUCAUAUUCCGCAUAAGACGGUCUUUGCUUUUUGUCUGGGAGUUAAGGCUGUAUUUAACAGACUCGCUAACUAUUACCCUGGCUAAUUGGGGAAUAGUCAAGCUAAACUCAAAUAGAUUAGCCUAAGCAUGUAGAUCCAAAGAUCUAGAGUUUGUGGACGCGGGUUCAAAUCCCGCCGCCUCCACCASEQ ID NO：164Guillardia theta(质体)ssrAGGGGCTGATTTGGATTCGACATATAAATTTGCGTGTTTCATTATGAAGCAAGTCAAGTTTAATGATCTTGTAAAAAACATTAAAGTACAAATAAATGCAAGCAATATAGTTTCATTTAGTTCAAAACGTTTAGTCTCTTTTGCATAAGCAAAATGTGTTAATAACTTTCTTAGTAGAAATTGGAGAAGTTTACTAAGATTTATATTTACTCCATAATTATTTTAAAGATGGTAAAAAGGTGATTCATCATTTGTATGTTTCTAAACTTTGTGAAAGAATAGTGGGCTCCATTTATAATGAACGTGGGTTCAAATCCCACCAGCTCCACCA  SEQ ID NO：165Guillardia theta(质体)tmRNAGGGGCUGAUUUGGAUUCGACAUAUAAAUUUGCGUGUUUCAUUAUGAAGCAAGUCAAGUUUAAUGAUCUUGUAAAAAACAUUAAAGUACAAAUAAAUGCAAGCAAUAUAGUUUCAUUUAGUUCAAAACGUUUAGUCUCUUUUGCAUAAGCAAAAUGUGUUAAUAACUUUCUUAGUAGAAAUUGGAGAAGUUUACUAAGAUUUAUAUUUACUCCAUAAUUAUUUUAAAGAUGGUAAAAAGGUGAUUCAUCAUUUGUAUGUUUCUAAACUUUGUGAAAGAAUAGUGGGCUCCAUUUAUAAUGAACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO：166Thalassiosira Weissflogii(质体)ssrAGGGGCTGATTTGGTTTCGACATTTAAAACTTCTTTCTATGTGTCAGGTCAAAGTTTGTATTCTTTGTAAAAAAATACTAAAATACTAATAAATGCTAATAATATAATACCGTTTATTTTTAAAGCAGTAAAAACAAAAAAAGAAGCAATGGCTTTAAATTTTGCTGTATAGTTCATTAACTTAGGTTATTAAATATTTTTTCATTATAACTGGACTTTTCTCTAGTTTATAGTTTAGAATAAATTTAAATTTTGCAAAACTCGTTCGAAAATTTTCGGGCTAAACCTGTAAACGCAAATACTAAGAAATTTTAGATGGACATGGGTTCAATTCCCATCAGTTCCACCASEQ ID NO：167Thalassiosira Weissflogii(质体)tmRNAGGGGCUGAUUUGGUUUCGACAUUUAAAACUUCUUUCUAUGUGUCAGGUCAAAGUUUGUAUUCUUUGUAAAAAAAUACUAAAAUACUAAUAAAUGCUAAUAAUAUAAUACCGUUUAUUUUUAAAGCAGUAAAAACAAAAAAAGAAGCAAUGGCUUUAAAUUUUGCUGUAUAGUUCAUUAACUUAGGUUAUUAAAUAUUUUUUCAUUAUAACUGGACUUUUCUCUAGUUUAUAGUUUAGAAUAAAUUUAAAUUUUGCAAAACUCGUUCGAAAAUUUUCGGGCUAAACCUGUAAACGCAAAUACUAAGAAAUUUUAGAUGGACAUGGGUUCAAUUCCCAUCAGUUCCACCA  SEQ ID NO：168幽门螺杆菌(Helicobacter pylori)ssrA，(临床分离物1)，内部部分TGGGGATGTTACGGTTTCGACAGGGGTAGTTCGAGCTTAGGTGGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAACTGGCAAACAACAAAACAACTTCGCTTTAGCAGCTTAATAAGCTCTTAGCGGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCAAATTAAGTGGGCTACGCTGGATTCCACCGTCTGAGGATGAAAGAAGAGAACAACCAGACTAGCTACCCGGACGCCCGTCGATAGGCAGATGGAGTAGCGAATCGCGAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTCTTGGACGTGGGTTCGACTCCCSEQ ID NO：176幽门螺杆菌(Helicobacter pylori)tmRNA，(临床分离物1)，内部部分UGGGGAUGUUACGGUUUCGACAGGGGUAGUUCGAGCUUAGGUGGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAACUGGCAAACAACAAAACAACUUCGCUUUAGCAGCUUAAUAAGCUCUUAGCGGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCAAAUUAAGUGGGCUACGCUGGAUUCCACCGUCUGAGGAUGAAAGAAGAGAACAACCAGACUAGCUACCCGGACGCCCGUCGAUAGGCAGAUGGAGUAGCGAAUCGCGAAUAUAUCGACUACACUCGUAGAAGCUUAAGUGCCGAUAUUCUUGGACGUGGGUUCGACUCCC  SEQ ID NO：177幽门螺杆菌(Helicobacter pylori)ssrA，(临床分离物2)，内部部分TGGGGACGTTACGGTTTCGACAGGGATAGTTCGAGCTTAGGTTGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAATTGGCAAACAAAACAATCTTTCTTTAGCTGCTTAATTGCACTAAAGGTTCCTCCCTCCATCGTCCATGTGGTAGGGTAAGGGACTCAAACTAAGTGGACTACGCCGGAGTTCGCCGTCTGAGGACAAAGGAAGAGAACAACCAGACTAGCAACTTGGAAGCCTGTCGATAGGCCGAAGAGTTCGCGAAATGCTAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTTTTGGACGTGGGTTCGATTCCCT  SEQ ID NO：178幽门螺杆菌(Helicobacter pylori)tmRNA，(临床分离物2)，内部部分UGGGGACGUUACGGUUUCGACAGGGAUAGUUCGAGCUUAGGUUGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAAUUGGCAAACAAAACAAUCUUUCUUUAGCUGCUUAAUUGCACUAAAGGUUCCUCCCUCCAUCGUCCAUGUGGUAGGGUAAGGGACUCAAACUAAGUGGACUACGCCGGAGUUCGCCGUCUGAGGACAAAGGAAGAGAACAACCAGACUAGCAACUUGGAAGCCUGUCGAUAGGCCGAAGAGUUCGCGAAAUGCUAAUAUAUCGACUACACUCGUAGAAGCUUAAGUGCCGAUAUUUUUGGACGUGGGUUCGAUUCCCUSEQ ID NO：179斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG  SEQ ID NO：180斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG  SEQ ID NO：181伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG  SEQ ID NO：182伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG  SEQ ID NO：183非洲分枝杆菌(Mycobacterium africanum)(临床分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA  SEQ ID NO：184非洲分枝杆菌(Mycobacterium africanum)(临床分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA  SEQ ID NO：185戈登氏分枝杆菌(Mycobacterium gordonae)(临床分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGTGTGACCAGGAGATCCGAGCAGAGACATAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA  SEQ ID NO：186戈登氏分枝杆菌(Mycobacterium gordonae)(临床分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGUGUGACCAGGAGAUCCGAGCAGAGACAUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA  SEQ ID NO：187堪萨斯分枝杆菌(Mycobacterium kansasii)(临床分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGACCGCCCTCGACCCGGACTCTGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCGTCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA  SEQ ID NO：188堪萨斯分枝杆菌(Mycobacterium kansasii)(临床分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGACCGCCCUCGACCCGGACUCUGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCGUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA  SEQ ID NO：189龟分枝杆菌(Mycobacterium chelonae)ssrA，内部部分ACAGCGAGTCTCGACTTAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCATTGCAACCAATTAAGCGCCGATTCTCATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGACCGGGAGCGCCCTCAGCCCGGACCCTGGCATCAGCTAGAGGGACAAACTACGGGTTCGGTCGCGGGACCCGTAGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGGGACCGAGAGATCCAAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTAATGAACGGCCGTTG  SEQ ID NO：190龟分枝杆菌(Mycobacterium chelonae)tmRNA，内部部分ACAGCGAGUCUCGACUUAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCAUUGCAACCAAUUAAGCGCCGAUUCUCAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGACCGGGAGCGCCCUCAGCCCGGACCCUGGCAUCAGCUAGAGGGACAAACUACGGGUUCGGUCGCGGGACCCGUAGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGGGACCGAGAGAUCCAAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUAAUGAACGGCCGUUG  SEQ ID NO：191斯氏分枝杆菌(苏加分枝杆菌)(Mycobacterium szulgai)(ATCC 35799)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGAGAACACTCAGCGCGACTTCGCTCTCGCTGCCTAAGCGACAGCAAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGACCCTGGCGTCATCTAGAGGGATCCACCGGTGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG  SEQ ID NO：192斯氏分枝杆菌(苏加分枝杆菌)(Mycobacterium szulgai)(ATCC 35799)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAGAACACUCAGCGCGACUUCGCUCUCGCUGCCUAAGCGACAGCAAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGACCCUGGCGUCAUCUAGAGGGAUCCACCGGUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG  SEQ ID NO：193玛尔摩分枝杆菌(Mycobacterium malmoense)(临床分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGTTTCAACACAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTGGAGGGATCCACCGGTGAGTCCGGTCGCGGGACTCATCGGGACATACACAGCGACTGGGATCGTCATCCTGGCTGGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG  SEQ ID NO：194玛尔摩分枝杆菌(Mycobacterium malmoense)(临床分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGUUUCAACACAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUGGAGGGAUCCACCGGUGAGUCCGGUCGCGGGACUCAUCGGGACAUACACAGCGACUGGGAUCGUCAUCCUGGCUGGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG  SEQ ID NO：195微黄分枝杆菌(Mycobacterium flavescens)ssrA，内部部分ACTTCGAGCGTCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGCCCGGGAGAGCCCTCGACCCGGTGTCTGGCATCAGCTAGAGGGATAAACCGGTGGGTCCGGTCGCGGGACTCATCGGGACATCAAACAGCGACTGGGATCGTCATCCTGACTTGTTCGCGTGATCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAACGCCGTAG  SEQ ID NO：196微黄分枝杆菌(Mycobacterium flavescens)tmRNA，内部部分ACUUCGAGCGUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGCCCGGGAGAGCCCUCGACCCGGUGUCUGGCAUCAGCUAGAGGGAUAAACCGGUGGGUCCGGUCGCGGGACUCAUCGGGACAUCAAACAGCGACUGGGAUCGUCAUCCUGACUUGUUCGCGUGAUCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAACGCCGUAG  SEQ ID NO：197海分枝杆菌(Mycobacterium marinum)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGATGCAACTAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGGCTCATCGGGACATCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGACCTAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG  SEQ ID NO：198海分枝杆菌(Mycobacterium marinum)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGAUGCAACUAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGGCUCAUCGGGACAUCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGACCUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG  SEQ ID NO：199田鼠分枝杆菌(Mycobacterium microti)(环境分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAGCCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA  SEQ ID NO：200田鼠分枝杆菌(Mycobacterium microti)(环境分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAGCCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA  SEQ ID NO：201耻垢分枝杆菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，内部部分ACTTCGAGCATCGAATCCAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTAAGCGACGGCTGGTCTGTCAGACCGGGAGTGCCCTCGGCCCGGATCCTGGCATCAGCTAGAGGGACCCACCCACGGGTTCGGTCGCGGGACCTGTGGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTCGAGGACATGCCGTAG  SEQ ID NO：202耻垢分枝杆菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，内部部分ACUUCGAGCAUCGAAUCCAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUAAGCGACGGCUGGUCUGUCAGACCGGGAGUGCCCUCGGCCCGGAUCCUGGCAUCAGCUAGAGGGACCCACCCACGGGUUCGGUCGCGGGACCUGUGGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGUGUGACCGGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUCGAGGACAUGCCGUAG  SEQ ID NO：203蟾(蜍)分枝杆菌(Mycobacterium xenopi)(临床分离物)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACTAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGGCCGGGAGTTCCCTCGACCCGGATCCTGGCGTCAGCTAGAGGGATCCACCGATGGGTTCGGTCGCGGGACCCATCGGGACACCACACAGCGACTGGGATCGCCGTCCCGGCTAGTTCGCGAGACCGGGAGATCCGAGTAAGGGCAAAGCGAACTGCGCACGGAGAAGCCTTGAGGGTATGCCGTA  SEQ ID NO：204蟾(蜍)分枝杆菌(Mycobacterium xenopi)(临床分离物)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACUAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGGCCGGGAGUUCCCUCGACCCGGAUCCUGGCGUCAGCUAGAGGGAUCCACCGAUGGGUUCGGUCGCGGGACCCAUCGGGACACCACACAGCGACUGGGAUCGCCGUCCCGGCUAGUUCGCGAGACCGGGAGAUCCGAGUAAGGGCAAAGCGAACUGCGCACGGAGAAGCCUUGAGGGUAUGCCGUA  SEQ ID NO：205胞内分枝杆菌(Mycobacterium intracellulare)(NCTC 10425)ssrA，内部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACCGACCACCGTAAGCGTCGTTGCAAACAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTTATCGGGACACCAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTAG  SEQ ID NO：206胞内分枝杆菌(Mycobacterium intracellulare)(NCTC 10425)tmRNA，内部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACCGACCACCGUAAGCGUCGUUGCAAACAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUUAUCGGGACACCAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUAG  SEQ ID NO：207淋巴腺炎分枝杆菌(瘰疬分枝杆菌)(Mycobacterium scrofulaceum)(NCTC 10803)ssrA，内部部分ACATCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGGACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTTG  SEQ ID NO：208淋巴腺炎分枝杆菌(瘰疬分枝杆菌)(Mycobacterium scrofulaceum)(NCTC 10803)tmRNA，内部部分ACAUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGGACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUUG  SEQ ID NO：209星状诺卡氏菌(Nocardia asteroides)ssrA，内部部分ACTGTGTGCGCCGAGGTAGGGGAAGCGTGTCGGTGCAGGCTGGAGACCACCGTTAAGCGTCGCGGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTGATCAGCGACGGCTAGCTGTCGGCCCGGGTTGTGTTCCCGAACCCGGATGCCGGCATCATCTCAGGGAACTCACCGTGTTCGCCGGTCGCGGACGGACACGGGACAGCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCAAGTAGAGACATAGCGGACTGCACACGGAGAAGCCCTACTGACTCGACACAG  SEQ ID NO：210星状诺卡氏菌(Nocardia asteroides)tmRNA，内部部分ACUGUGUGCGCCGAGGUAGGGGAAGCGUGUCGGUGCAGGCUGGAGACCACCGUUAAGCGUCGCGGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUGAUCAGCGACGGCUAGCUGUCGGCCCGGGUUGUGUUCCCGAACCCGGAUGCCGGCAUCAUCUCAGGGAACUCACCGUGUUCGCCGGUCGCGGACGGACACGGGACAGCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCAAGUAGAGACAUAGCGGCUGCACACGGAGAAGCCCUACUGACUCGACACAG  SEQ ID NO：211肠炎沙门氏菌(Salmonella enteritidis)ssrA，内部部分ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGG  SEQ ID NO：212肠炎沙门氏菌(Salmonella enteritidis)tmRNA，内部部分ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG  SEQ ID No：213表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，内部部分ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO：214表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，内部部分ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUAUAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG  SEQ ID NO：215无乳链球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，内部部分ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGG  SEQ ID NO：216无乳链球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，内部部分ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG  SEQ ID NO：217
上面所提到的序列SEQ ID NO：47-62，65-68，71和72，98和99，159-168和176-217为新序列。
上面所提到的序列能够用于形成ssrA基因序列的数据库，可用来鉴定细菌种类或产生核酸诊断检验盒。
下面为与本发明相应的代表性探针：
沙门氏菌：
1)属特异性探针：
5’-CGAATCAGGCTAGTCTGGTAG-3’ SEQ ID NO：218
分枝杆菌：
2)用于检测肺结核复合物的寡聚核苷酸探针：
TB01 5’-ACTCCTCGGACA(A/G)CCACAGCGA-3’SEQ ID NO：219
3)用于检测鸟分枝杆菌和鸟分枝杆菌(副结核亚种)序列的寡核苷酸探针
探针1：PAV1-5’-GTTGCAAATAGATAAGCGCC-3’SEQ IDNO：220
探针2：PAV2-5’-TCCGTCAGCCCGGGAACGCC-3’SEQID NO：221
李斯特氏菌
4)用于测定热杀灭处理细胞后tmRNA完整性的寡核苷酸探针
LVtm：5′-TTTTGTTTTTCTTTGCCA-3′SEQ ID NO：222
大肠杆菌：
5)用于测定热杀死处理细胞后tmRNA完整性的寡核苷酸探针
Evtm：5′-AGTTTTCGTCGTTTGCGA-3′SEQ ID NO：223
下面为根据本发明的具代表性的引物：
分枝杆菌：
1)用于扩增所有分枝杆菌序列的简并引物
5′引物
10SAAM3-5′-CAGGCAA(G/C)(A/T/C)GACCACCGTAA-3′SEQ ID NO：224
3′引物
10SAAM4-5′GGATCTCC(C/T)G(A/G)TC(A/T)C(A/G)CG(A/G)AC(A/T)A-3′SEQ ID NO：225
2)用于检测鸟分枝杆菌和鸟分枝杆菌(副结核亚种)序列的寡核苷酸引物
5’引物：AP1正向-5’-TGCCGGTGCAGGCAACTG-3’SEQ ID NO：226
3’引物：AP2反向-5’-CACGCGAACAAGCCAGGA-3’SEQ IDNO：227
附图简述
在附图中：
图1为大肠杆菌和霍乱弧菌ssrA基因序列的Clustal对比；
图2为从大肠杆菌和霍乱弧菌细胞中制备的全细胞RNA的琼脂糖凝胶照片；
图3为霍乱弧菌寡核苷酸探针与大肠杆菌和霍乱弧菌的tmRNA的转录序列杂交的放射自显影照片；
图4为用通用ssrA基因扩增引物从一系列生物体中得到的扩增产物的琼脂糖凝胶照片；
图5为李斯特氏菌ssrA基因序列的Clustal对比；
图6为单核细胞增多性李斯特氏菌和枯草芽孢杆菌ssrA/tmRNA基因序列的Clustal对比；
图7为用李斯特菌属特异性PCR扩增引物从一系列生物体中得到的扩增产物的琼脂糖凝胶照片；
图8为李斯特菌属特异性寡核苷酸探针与实施例4中制备的一系列生物体杂交的放射自显影照片；
图9为单核细胞增多性李斯特氏菌种特异性寡核苷酸探针与实施例7中制备的一系列生物体杂交的放射自显影照片；
图10为如实施例6中所述的用多色探针检测方法检测尼龙膜上李斯特氏菌ssrA基因的计算机扫描图象；
图11为沙眼衣原体菌株的ssrA基因序列的Clustal对比；
图12为幽门螺杆菌菌株ssrA基因序列的Clustal对比；
图13为生殖道枝原体菌株ssrA基因序列的Clustal对比；
图14为淋病奈瑟球菌菌株ssrA基因序列的Clustal对比；
图15为单核细胞增多性李斯特氏菌菌株ssrA基因序列的Clustal对比；
图16为单核细胞增多性李斯特氏菌和无害李斯特菌菌株的ssrA基因序列的Clustal对比
图17为李斯特菌寡聚核苷酸探针(Evtm)与经中度热处理的大肠杆菌细胞中分离的全RNA样品杂交的放射自显影照片；
图18为李斯特菌寡聚核苷酸探针(Evtm)与经极度热处理的大肠杆菌细胞中分离的全RNA样品杂交的放射自显影照片；
图19为李斯特氏菌寡聚核苷酸探针(Lvtm)与经中度热处理的单核细胞增多性李斯特氏菌细胞中分离的全RNA样品杂交的放射自显影照片；
图20为李斯特氏菌寡聚核苷酸探针(Lvtm)与经极度热处理的单核细胞增多性李斯特氏菌细胞中分离的全RNA样品杂交的放射自显影照片；
图21为经热处理后不同时间点tmRNA的RT-PCR产物的琼脂糖凝胶照片。
本发明将通过下面的实施例作进一步的阐明。完成本发明的方式实施例1
检查可用的tmRNA序列的初级核苷酸序列
用Clustal W核酸序列对比程序对可用的tmRNA进行比较性初级核酸序列对比，表明原核生物的tmRNA比其它细菌高拷贝数RNA具有更显著程度的核酸序列变异性和非同源性，如表1所示；
                         表1
       不同细菌的RNA分子间核苷酸序列同源性百分比
 大肠杆菌∶霍乱弧菌  枯草芽孢杆菌∶结核分枝杆菌
 rRNA％同源性     88     66
 tmRNA％同源性     68     25
这些不同细菌的tmRNA序列间的非同源性区位于分子的中间，tmRNA分子内核苷酸序列的非同源性程度表明可制备属和种特异性探针以用来区别和/或检测细菌。
核苷酸序列对比已经表明tmRNA分子的5’和3’侧翼区都有种内和属内的高度同源性。这表明可制备通用寡核苷酸引物，以用来从多种细菌中扩增ssrA基因或其编码的tmRNA。
我们已经证明来源于不同生物体的tmRNA分子的核苷酸序列内的同源区和非同源区可以被用来作为在分子水平鉴定和检测生物体的基础。实施例2
霍乱弧菌tmRNA特异性探针的设计
如图1所显示的大肠杆菌核苷酸序列(SEQ ID NO.37)和霍乱弧菌ssrA序列(SEQ ID NO.127)的对比表明这两种细菌在系统发生上有紧密的联系。但是这些序列间的非同源区由缺乏星号标明。合成了图1中加下划线的霍乱弧菌ssrA核苷酸序列可变区的互补的寡核苷酸探针。
霍乱弧菌tmRNA特异性探针的序列为：
5’-AACGAATGGCTAACCTGAA-3’SEQ ID NO.169
在大肠杆菌和霍乱弧菌生长的中间指数期和稳定期，从液体培养物中分离得到全RNA。如图2所示等量的被分离的全RNA在变性甲醛琼脂糖凝胶中电泳并被印迹到HYBOND-N尼龙膜上，其中泳道1-4分别代表：
泳道1：中间指数期大肠杆菌全RNA
泳道2：中间指数期霍乱弧菌全RNA
泳道3：稳定期大肠杆菌全RNA
泳道4：稳定期霍乱弧菌全RNA
将所得的Northern印迹与末端标记γP32的霍乱弧菌tmRNA特异性探针杂交。如图3所示的杂交试验的结果证明了探针的特异性，因为只有霍乱弧菌tmRNA被检测到。而且，观察到在生长稳定期的培养液中分离的霍乱弧菌tmRNA有更大程度的杂交信号强度，这表明在这一时期在霍乱弧菌细胞中存在更大拷贝数目的tmRNA分子。实施例3
用于表征未知ssrA基因和tmRNA序列的通用ssrA/tmRNA寡核苷酸扩增引物的产生
所有可用的ssrA基因和tmRNA序列的Clustal W对比表明能够设计出简并的寡核苷酸引物，用来从多种生物体中扩增ssrA基因和tmRNA核苷酸序列。
可合成简并的寡核苷酸引物，用来从大范围的细菌的全基因组DNA制备物中PCR扩增得到ssrA基因序列。
合成的简并寡核苷酸如下所示：
(a)tmU5’：5’体外PCR扩增引物
5’-GGG(A/C)(C/T)TACGG(A/T)TTCGAC-3’SEQ ID NO：170
(b)tmU5’：3’体外PCR扩增引物
5’-GGGA(A/G)TCGAACC(A/G)(C/G)GTCC-3’SEQ ID NO：171
括号内为简并碱基位置。
PCR反应的产物在琼脂糖凝胶中电泳，如图4所示在所有例子中都扩增得到约350碱基对PCR产物，这表明简并tmRNA引物的通用性。
图4中的各泳道分别代表：
A：分子量标记V
1：大肠杆菌
2：沙门氏菌浦纳亚种
3：产气克雷伯氏菌
4：奇异变形菌
5：雷氏变形菌
6：气单胞菌
7：金黄色葡萄球菌
8：肠球菌模式种(粪链球菌)
9：乳杆菌模式种
10：枯草芽孢杆菌
11：单核细胞增多性李斯特氏菌
12：无害李斯特菌
13：墨氏李斯特菌
14：威氏李斯特菌
15：格氏李斯特菌
16：牛分枝杆菌
B：分子量标记V
如表2所示，所述通用引物从革兰氏阳性和革兰氏阴性细菌中均扩增到了ssrA基因。
                         表2
            用通用扩增引物进行细菌物种检测
    PCR产物
革兰氏阴性菌   大肠杆菌     +
  沙门氏菌浦纳亚种     +
  产气克雷伯氏菌     +
  奇异变形菌     +
  雷氏变形菌     +
  气单胞菌     +
革兰氏阳性菌   金黄色葡萄球菌     +
  肠球菌模式种     +
  乳杆菌模式种     +
  枯草芽孢杆菌     +
  单核细胞增多性李斯特氏菌     +
  无害李斯特菌     +
  墨氏李斯特菌     +
  威氏李斯特菌     +
  格氏李斯特菌     +
  牛分枝杆菌     +
实施例4
分离和表征以前未知的细菌ssrA/tmRNA核苷酸序列
由实施例2，从李斯特菌和牛分枝杆菌的基因组DNA中扩增得到的PCR产物被亚克隆到T末端质粒载体来进行DNA测序。每个物种挑选3个重组克隆并用双脱氧测序法进行测序。对每个亚克隆的DNA双链的序列进行测定。
牛分枝杆菌ssrA基因的核苷酸序列与肺结核分枝杆菌ssrA基因序列有100％同源性。
图5所示为从李斯特菌得到的新ssrA基因序列(SEQ ID NOS 51，53，55，59和61)的Clustal W对比。该分析表明能够产生用于李斯特菌的属特异性探针和寡核苷酸扩增引物。而且，该对比还表明可产生种特异性寡核苷酸探针，并能够从其它李斯特菌中区分出单核细胞增多性李斯特氏菌。
在图5中，据推测的属特异性寡核苷酸引物Ltm1和Ltm2被框出，同时作为属特异性李斯特菌寡核苷酸探针的LGtM也被框出。据推测的单核细胞增多性李斯特氏菌种特异性寡核苷酸探针序列以下划线和斜体标识。
为进一步说明ssrA基因/tmRNA核酸靶点为适合细菌诊断的靶点，进行了单核细胞增多性李斯特氏菌ssrA基因核苷酸序列(SEQ IDNO.55)和枯草芽孢杆菌ssrA基因核苷酸序列(SEQ ID NO.11)(一种和李斯特氏菌在种系发生上密切相关的细菌)的比较性对比，如图6所示。对该序列对比进行序列分析表明其序列同源性为41％，相应的16S rRNA对比表明核苷酸序列有87％的同源性(数据没有出示)。实施例5
用于李斯特氏菌菌种的ssrA基因/tmRNA属特异性扩增引物，属特异性和种特异性探针的产生和应用
用实施例4中李斯特氏菌的ssrA基因/tmRNA核苷酸序列对比，分析ssrA基因/tmRNA核苷酸序列区域以决定其是否适用于产生属特异性扩增引物和属特异性及种特异性寡核苷酸探针。在分析中，表现出与枯草芽孢杆菌有最大程度差异的区域被选出来设计这些扩增引物和探针。
下面为合成的寡核苷酸的序列：
(a)Ltm1：5’李斯特氏菌属特异性扩增引物
5’-AAAGCCAATAATAACTGG-3’SEQ ID NO：172
(b)Ltm2：3’李斯特氏菌属特异性扩增引物
5’-CCAGAAATATCGGCACTT-3’SEQ ID NO：173
(c)LGtm：李斯特氏菌属特异性杂交探针
5’-GTGAGACCCTTACCGTAG-3’SEQ ID NO：174
(d)LStm：单核细胞增多性李斯特氏菌种特异性杂交探针
5’-TCTATTTAACCCCAGACG-3’SEQ ID NO：175
属特异性扩增引物Ltm1和Ltm2用于与做为模板的二十个不同菌株的全基因组DNA进行一系列PCR反应。用这些引物只能从李斯特氏菌中扩增得到ssrA基因序列(260碱基对产物)(图7和表3)，这表明ssrA基因/tmRNA适合于作为体外扩增某细菌属的靶点。没有检测到其它细菌的扩增产物，尽管采用如实施例2所描述的通用引物(tmU5’和tmU3’)从这些细菌的DNA中PCR扩增得到了PCR产物。
图7中的各泳道分别代表：
A：分子量标记V
1：大肠杆菌
2：沙门氏菌浦纳亚种
3：产气克雷伯氏菌
4：奇异变形菌
5：雷氏变形菌
6：气单胞菌
7：金黄色葡萄球菌
8：肠球菌模式种
9：乳杆菌模式种
10：枯草芽孢杆菌
11：单核细胞增多性李斯特氏菌 菌株1
12：单核细胞增多性李斯特氏菌 菌株2
13：单核细胞增多性李斯特氏菌 菌株3
14：单核细胞增多性李斯特氏菌 菌株4
15：单核细胞增多性李斯特氏菌 临床分离物
16：无害李斯特菌
17：墨氏李斯特菌
18：威氏李斯特菌
19：格氏李斯特菌
20：牛分枝杆菌
B：分子量标记V
                      表3
   用李斯特氏菌特异性扩增引物进行细菌种的检测
 PCR产物
革兰氏阴性菌  大肠杆菌  -
 沙门氏菌浦纳亚种  -
 产气克雷伯氏菌  -
 奇异变形菌  -
 雷氏变形菌  -
 气单胞菌  -
革兰氏阳性菌  金黄色葡萄球菌  -
 肠球菌模式种  -
 乳杆菌模式种  -
 枯草芽孢杆菌  -
 李斯特氏菌 菌株1  +
 单核细胞增多性李斯特氏菌 菌株2  +
 单核细胞增多性李斯特氏菌 菌株3  +
 单核细胞增多性李斯特氏菌 菌株4  +
 单核细胞增多性李斯特氏菌临床分离物  +
 无害李斯特菌  +
 墨氏李斯特菌  +
 威氏李斯特菌  +
 格氏李斯特菌  +
 牛分枝杆菌  -
如图4所示，李斯特氏菌属特异性寡核苷酸探针LGtM进行Southern印迹杂交。如图8和表4所示，只在各李斯特氏菌种中观察到阳性杂交信号，表明tmRNA序列可以作为检测特定属的靶点。
图8中所示的各泳道为：
A：分子量标记V
1：大肠杆菌
2：沙门氏菌浦纳亚种
3：产气克雷伯氏菌
4：奇异变形菌
5：雷氏变形菌
6：气单胞菌
7：金黄色葡萄球菌
8：肠球菌模式种
9：乳杆菌模式种
10：枯草芽孢杆菌
11：单核细胞增多性李斯特氏菌
12：无害李斯特菌
13：墨氏李斯特菌
14：威氏李斯特菌
15：格氏李斯特菌
16：牛分枝杆菌
B：分子量标记V
用本实施例所描述的属特异性扩增得到的PCR产物，如图7所示，进行Southern印迹，其与单核细胞增多性李斯特氏菌种特异性寡核苷酸探针杂交。如图9和表4所示在四株单核细胞增多性李斯特氏菌中的三株和一株临床分离得到的菌株检测到阳性杂交信号。
图9中的各泳道代表：
A：分子量标记V
1：大肠杆菌
2：沙门氏菌浦纳亚种
3：产气克雷伯氏菌
4：奇异变形菌
5：雷氏变形菌
6：气单胞菌
7：金黄色葡萄球菌
8：肠球菌模式种
9：乳杆菌模式种
10：枯草芽孢杆菌
11：单核细胞增多性李斯特氏菌 菌株1
12：单核细胞增多性李斯特氏菌 菌株2
13：单核细胞增多性李斯特氏菌 菌株3
14：单核细胞增多性李斯特氏菌 菌株4
15：单核细胞增多性李斯特氏菌 临床分离物
16：无害李斯特菌
17：墨氏李斯特菌
18：威氏李斯特菌
19：格氏李斯特菌
20：牛分枝杆菌
B：分子量标记V
                        表4
             李斯特氏菌属特异性探针和
   单核细胞增多性李斯特氏菌种特异性探针的特异性
    LGtm属特异性探针     LStm种特异性针
革兰氏阴性菌  大肠杆菌     -     -
 沙门氏菌浦纳亚种     -     -
 产气克雷伯氏菌     -     -
 奇异变形菌     -     -
 雷氏变形菌     -     -
 气单胞菌     -     -
革兰氏阳性菌  金黄色葡萄球菌     -     -
 肠球菌     -     -
 乳杆菌模式种     -     -
 枯草芽孢杆菌     -     -
 单核细胞增多性李斯特氏菌 菌株1     +     +
 单核细胞增多性李斯特氏菌 菌株2     +     +
 单核细胞增多性李斯特氏菌 菌株3     +     +
 单核细胞增多性李斯特氏菌 菌株4     +     -
 单核细胞增多性李斯特氏菌 临床分离物     +     +
 无害李斯特菌     +     -
 墨氏李斯特菌     +     -
 威氏李斯特菌     +     -
 格氏李斯特菌     +     -
 牛分枝杆菌     -     -
菌株4，一种典型的单核细胞增多性李斯特氏菌菌株，用这种探针不能产生阳性信号。对从该菌株中PCR扩增得到的ssrA基因进行DNA测序表明其含有与无害李斯特氏菌相同的探针靶点区。但是应该注意到从该菌株得到的ssrA基因包含的其它区域的序列与前述的单核细胞增多性李斯特氏菌中的这些序列相同而不同于无害李斯特氏菌，如图15所示。所以可合成针对这些可变区其中之一的种特异性寡核苷酸探针，该探针或在种(例如单核细胞增多性李斯特氏菌)内识别每种株型(分离物)。实施例6
李斯特氏菌ssrA基因序列的多色探针检测
将LGTm(A)，LStm(B)和乌普萨拉弯曲杆菌的16S-23S rRNA间隔序列(C-5′CATTAAACTTTAGCAAGGAAGTG 3′SEQ ID NO：228)寡核苷酸探针非可逆地结合到尼龙膜上与扩增的ssrA PCR产物进行杂交，所述扩增利用属特异性引物Ltm1 and Ltm2(Ltm1的5′端标记有生物素)，它们来源于单核细胞增多性李斯特氏菌(1-6)，无害李斯特菌(7-10)，伊氏李斯特氏菌(11)，墨氏李斯特氏菌(12)，斯氏李斯特氏菌(13)，威氏李斯特氏菌(14)和格氏李斯特氏菌(15)。用tmU5′和tmU3′(tmU5′的5′端标记有生物素)扩增得到的ssrA的PCR扩增产物也被杂交到尼龙膜条带上，被检测细菌来源于革兰氏阳性菌枯草芽孢杆菌、乳杆菌模式种、金黄色葡萄球菌、表皮葡萄球菌、肠球菌模式种、产气荚膜梭菌(16-21)和革兰氏阴性菌大肠杆菌、肠炎沙门氏菌、雷氏变形菌、产气克雷伯氏菌(22-25)。如图10所示，经杂交后，对生物素比色检验进行显影表明：条带1-6表明ssrA的PCR扩增产物来自于单核细胞增多性李斯特氏菌并证明PCR扩增产物来自李斯特氏菌，因A和B都能够进行颜色检测；条带7-15证明PCR产物来自于李斯特氏菌，但其中只有A能够进行颜色检测；条带16-25表明PCR产物不是从李斯特氏菌中得到的，因没有检测到颜色。C是阴性寡核苷酸对照探针而D为对所有样品的比色检验的阳性对照。实施例7
用ssrA/tmRNA序列来区分生物体的物种
图11(SEQ ID NOS.19和21)，图12(SEQ ID NOS.41和43)，图13(SEQ ID NOS.77和79)，图14(SEQ ID NOS.83和85)15和16(SEQ ID NO.53，55和57)所示的Clustal W序列对比表明在同一种细菌的不同菌株的ssrA/tmRNA序列内存在着核苷酸的差异。这表明ssrA/tmRNA序列潜在地可以用来区分同一细菌种类中不同菌株的个体和/或集体。这有助于其应用于流行病学研究和细菌种群分析。实施例8
对细菌细胞进行中度和极度热处理后分析tmRNA的完整性
对培养物进行热处理：将大肠杆菌和单核细胞增多性李斯特氏菌在80℃进行热处理，对于大肠杆菌而言处理20分钟，对于单核细胞增多性李斯特氏菌处理40分钟，以及于120℃处理15分钟(高压釜)。经过过夜生长和热处理后在0小时，1小时，2小时，6小时，12小时和48小时进行测验。每个时期的检测都没观察到存活的菌体。在这些时期全RNA被分离并在变性的1.2％琼脂糖凝胶中电泳及进行Northern印迹。在大肠杆菌的例子中(图17和图18)每个印迹都会与放射性标记的寡核苷酸探针Evtm杂交，在单核细胞增多性李斯特氏菌例子中(图19和图20)印迹与放射性标记的LVtm杂交。在每个检测样品中没有检测到tmRNA转录序列，这表明热处理后tmRNA转录序列已被降解。带有符号+ve的泳道为全RNA样品的阳性对照。实施例9
用tmRNA转录序列来区别活的和死的细菌
100ml单核细胞增多性李斯特氏菌在液体培养液中培养过夜。生长后，对细胞进行连续稀释并通过在营养琼脂平板上铺板来测定存活率。同时，从这些细胞的1ml液体中分离得到全RNA。剩余的细胞在65℃热处理20分钟。接着将细胞除去来进行存活率分析和全RNA分离。在处理后0小时，2小时，6小时和24小时的时间点取样品来测定存活率和RNA分离。
在营养琼脂平板上铺板表明热处理能杀死单核细胞增多性李斯特氏菌细胞，因没有观察到活的集落形成单位。每个分离的RNA样品用DNA酶处理以除去所含的任何DNA并且用李斯特氏菌属特异性ssrA/tmRNA寡核苷酸引物Ltm1和Ltm2对全RNA样品(100ng)进行反转录PCR扩增。扩增反应的阴性对照包括引物、靶序列和Taq聚合酶，但没有反转录酶。
扩增反应的结果如图12所示。
扩增的tmRNA的RT-PCR产物只能在没有经热处理的RNA样品中检测到。所有其它样品中没有产生RT-PCR产物，这表明这些样品中tmRNA分子在热处理后死的细胞中已经被降解。
图21中的各泳道代表：
A：分子量标记V；
1：RNA的PCR扩增(没有热处理过的细胞)
   反转录酶(RT)，+Taq聚合酶(TP)；
2：RNA的RT-PCR(没有经过热处理的细胞)，+RT，+TP；
3：RNA的PCR扩增(热处理后0小时)，-RT，+TP；
4：RNA的RT-PCR(热处理后0小时)，+RT，+TP；
5：RNA的PCR扩增(热处理后1小时)，-RT，+TP；
6：RNA的RT-PCR(热处理后1小时)，+RT，+TP；
7：RNA的PCR扩增(热处理后2小时)，-RT，+TP；
8：RNA的RT-PCR(热处理后2小时)，+RT，+TP；
9：RNA的PCR扩增(热处理后6小时)，-RT，+TP；
10：RNA的RT-PCR(热处理后6小时)，+RT，+TP；
11：RNA的PCR扩增(热处理后24小时)，-RT，+TP；
12：RNA的RT-PCR(热处理后24小时)，+RT，+TP；
B：分子量标记
                            序列表<110> 爱尔兰公司(爱尔兰生物研究)等人<120> 应用于原核生物和真核生物的基于核酸探针的诊断检验<130> P00-37-PCT<140><141><150> PCT/IE 99/00043<151> 1999-05-14<160> 228<170> PatentIn Ver.2.0<210> 1<211> 366<212> DNA<213> Actinobacillus actinomycetemcomitans<400> 1ggggctgatt ctggattcga cgggattagc gaagcccgaa gtgcacgtcg aggtgcggta 60ggcctcgtaa ataaaccgca aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc 120ttaataacct gcctttagcc ttcgctcccc agcttccgct cgtaagacgg ggataaagcg 180gagtcaaacc aaaacgagat cgtgtggaag ccaccgtttg aggatcgaag cattaaatta 240aatcaaagta gcttaattgt cgcgtgtccg tcagcaggat taagtgaatt taaagaccgg 300actaaacgtg tagtgctaac ggcagaggaa tttcggacgg gggttcaact ccccccagct 360ccacca                                                            366<210> 2<211> 366<212> RNA<213> Actinobacillus actinomycetemcomitans<400> 2ggggcugauu cuggauucga cgggauuagc gaagcccgaa gugcacgucg aggugcggua 60ggccucguaa auaaaccgca aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc 120uuaauaaccu gccuuuagcc uucgcucccc agcuuccgcu cguaagacgg ggauaaagcg 180gagucaaacc aaaacgagau cguguggaag ccaccguuug aggaucgaag cauuaaauua 240aaucaaagua gcuuaauugu cgcguguccg ucagcaggau uaagugaauu uaaagaccgg 300acuaaacgug uagugcuaac ggcagaggaa uuucggacgg ggguucaacu ccccccagcu 360ccacca                                                            366<210> 3<211> 315<212> DNA<213> Aeromonas salmonicida<400> 3aagattcacg aaacccaagg tgcatgccga ggtgcggtag gcctcgttaa caaaccgcaa 60aaaaatagtc gcaaacgacg aaaactacgc actagcagct taataacctg catagagccc 120ttctacccta gcttgcctgt gtcctaggga atcggaaggt catccttcac aggatcgtgt 180ggaagtcctg ctcggggcgg aagcattaaa accaatcgag ctagtcaatt cgtggcgtgt 240ctctccgcag cgggttggcg aatgtaaaga gtgactaagc atgtagtacc gaggatgtag 300taattttgga cgggg                                                  315<210> 4<211> 315<212> RNA<213> Aeromonas salmonicida<400> 4aagauucacg aaacccaagg ugcaugccga ggugcgguag gccucguuaa caaaccgcaa 60aaaaauaguc gcaaacgacg aaaacuacgc acuagcagcu uaauaaccug cauagagccc 120uucuacccua gcuugccugu guccuaggga aucggaaggu cauccuucac aggaucgugu 180ggaaguccug cucggggcgg aagcauuaaa accaaucgag cuagucaauu cguggcgugu 240cucuccgcag cggguuggcg aauguaaaga gugacuaagc auguaguacc gaggauguag 300uaauuuugga cgggg                                                  315<210> 5<211> 349<212> DNA<213> Alcaligenes eutrophus<400> 5tgggccgacc tggtttcgac gtggttacaa agcagtgagg cataccgagg acccgtcacc 60tcgttaatca atggaatgca ataactgcta acgacgaacg ttacgcactc gcttaattgc 120ggccgtcctc gcactggctc gctgacgggc tagggtcgca agaccacgcg aggtatttac 180gtcagataag ctccggaagg gtcacgaagc cggggacgaa aacctagtga ctcgccgtcg 240tagagcgtgt tcgtccgatg cgccggttaa atcaaatgac agaactaagt atgtagaact 300ctctgtggag ggcttacgga cgcgggttcg attcccgccg gctccacca             349<210> 6<211> 349<212> RNA<213> Alcaligenes eutrophus<400> 6ugggccgacc ugguuucgac gugguuacaa agcagugagg cauaccgagg acccgucacc 60ucguuaauca auggaaugca auaacugcua acgacgaacg uuacgcacuc gcuuaauugc 120ggccguccuc gcacuggcuc gcugacgggc uagggucgca agaccacgcg agguauuuac 180gucagauaag cuccggaagg gucacgaagc cggggacgaa aaccuaguga cucgccgucg 240uagagcgugu ucguccgaug cgccgguuaa aucaaaugac agaacuaagu auguagaacu 300cucuguggag ggcuuacgga cgcggguucg auucccgccg gcuccacca             349<210> 7<211> 347<212> DNA<213> Aquifex aeolicus<400> 7gggggcggaa aggattcgac ggggacaggc ggtccccgag gagcaggccg ggtggctccc 60gtaacagccg ctaaaacagc tcccgaagct gaactcgctc tcgctgccta attaaacggc 120agcgcgtccc cggtaggttt gcgggtggcc taccggaggg cgtcagagac acccgctcgg 180gctactcggt cgcacggggc tgagtagctg acacctaacc cgtgctaccc tcggggagct 240tgcccgtggg cgacccgagg ggaaatcctg aacacgggct aagcctgtag agcctcggat 300gtggccgccg tcctcggacg cgggttcgat tcccgccgcc tccacca               347<210> 8<211> 347<212> RNA<213> Aquifex aeolicus<400> 8gggggcggaa aggauucgac ggggacaggc gguccccgag gagcaggccg gguggcuccc 60guaacagccg cuaaaacagc ucccgaagcu gaacucgcuc ucgcugccua auuaaacggc 120agcgcguccc cgguagguuu gcggguggcc uaccggaggg cgucagagac acccgcucgg 180gcuacucggu cgcacggggc ugaguagcug acaccuaacc cgugcuaccc ucggggagcu 240ugcccguggg cgacccgagg ggaaauccug aacacgggcu aagccuguag agccucggau 300guggccgccg uccucggacg cggguucgau ucccgccgcc uccacca               347<210> 9<211> 316<212> DNA<213> Bacillus megaterium<400> 9agggtagttc gagcttaggt tgcgagtcga ggagatggcc tcgttaaaac atcaacgcca 60ataataactg gcaaatctaa caataacttc gctttagctg cataatagta gcttagcgtt 120cctccctcca tcgcccatgt ggtagggtaa gggactcact ttaagtgggc tacgccggag 180ttcgccgtct gaggacgaag gaagagaata atcagactag cgactgggac gcctgttggt 240aggcagaaca gctcgcgaat gatcaatatg ccaactacac tcgtagacgc ttaagtggcc 300atatttctgg acgtgg                                                 316<210> 10<211> 316<212> RNA<213> Bacillus megaterium<400> 10aggguaguuc gagcuuaggu ugcgagucga ggagauggcc ucguuaaaac aucaacgcca 60auaauaacug gcaaaucuaa caauaacuuc gcuuuagcug cauaauagua gcuuagcguu 120ccucccucca ucgcccaugu gguaggguaa gggacucacu uuaagugggc uacgccggag 180uucgccgucu gaggacgaag gaagagaaua aucagacuag cgacugggac gccuguuggu 240aggcagaaca gcucgcgaau gaucaauaug ccaacuacac ucguagacgc uuaaguggcc 300auauuucugg acgugg                                                 316<210> 11<211> 363<212> DNA<213> Bacillus subtilis<400> 11ggggacgtta cggattcgac agggatggat cgagcttgag ctgcgagccg agaggcgatc 60tcgtaaacac gcacttaaat ataactggca aaactaacag ttttaaccaa aacgtagcat 120tagctgccta ataagcgcag cgagctcttc ctgacattgc ctatgtgtct gtgaagagca 180catccaagta ggctacgctt gcgttcccgt ctgagaacgt aagaagagat gaacagacta 240gctctcggaa ggcccgcccg caggcaagaa gatgagtgaa accataaata tgcaggctac 300gctcgtagac gcttaagtaa tcgatgtttc tggacgtggg ttcgactccc accgtctcca 360cca                                                               363<210> 12<211> 363<212> RNA<213> Bacillus subtilis<400> 12ggggacguua cggauucgac agggauggau cgagcuugag cugcgagccg agaggcgauc 60ucguaaacac gcacuuaaau auaacuggca aaacuaacag uuuuaaccaa aacguagcau 120uagcugccua auaagcgcag cgagcucuuc cugacauugc cuaugugucu gugaagagca 180cauccaagua ggcuacgcuu gcguucccgu cugagaacgu aagaagagau gaacagacua 240gcucucggaa ggcccgcccg caggcaagaa gaugagugaa accauaaaua ugcaggcuac 300gcucguagac gcuuaaguaa ucgauguuuc uggacguggg uucgacuccc accgucucca 360cca                                                               363<210> 13<211> 387<212> DNA<213> Bordetella pertussis<400> 13ggggccgatc cggattcgac gtgggtcatg aaacagctca gggcatgccg agcaccagta 60agctcgttaa tccactggaa cactacaaac gccaacgacg agcgtctcgc tctcgccgct 120taagcggtga gccgctgcac tgatctgtcc ttgggtcagg cgggggaagg caacttcaca 180gggggcaacc ccgaaccgca gcagcgacat tcacaaggaa tcggccaccg ctggggtcac 240acggcgttgg tttaaattac gtgaatcgcc ctggtccggc ccgtcgatcg gctaagtcca 300gggttaaatc caaatagatc gactaagcat gtagaactgg ttgcggaggg cttgcggacg 360ggggttcaat tccccccggc tccacca                                     387<210> 14<211> 387<212> RNA<213> Bordetella pertussis<400> 14ggggccgauc cggauucgac gugggucaug aaacagcuca gggcaugccg agcaccagua 60agcucguuaa uccacuggaa cacuacaaac gccaacgacg agcgucucgc ucucgccgcu 120uaagcgguga gccgcugcac ugaucugucc uugggucagg cgggggaagg caacuucaca 180gggggcaacc ccgaaccgca gcagcgacau ucacaaggaa ucggccaccg cuggggucac 240acggcguugg uuuaaauuac gugaaucgcc cugguccggc ccgucgaucg gctaagucca 300ggguuaaauc caaauagauc gacuaagcau guagaacugg uugcggaggg cuugcggacg 360gggguucaau uccccccggc uccacca                                     387<210> 15<211> 362<212> DNA<213> Borrelia burgdorferi<400> 15ggggatgttt tggatttgac tgaaaatgtt aatattgtaa gttgcaggca gagggaatct 60cttaaaactt ctaaaataaa tgcaaaaaat aataacttta caagctcaaa tcttgtaatg 120gctgcttaag ttagcagagg gttttgttga atttggcttt gaggttcact tatactcttt 180tcgacatcaa agcttgctta aaaatgtttt caagttgatt tttagggact tttatacttg 240agagcaattt ggtggtttgc tagtatttcc aaaccatatt gcttaataaa atactagata 300agcttgtaga agcttatagt attattttta ggacgcgggt tcaattcccg ccatctccac 360ca                                                                362<210> 16<211> 362<212> RNA<213> Borrelia burgdorferi<400> 16ggggauguuu uggauuugac ugaaaauguu aauauuguaa guugcaggca gagggaaucu 60cuuaaaacuu cuaaaauaaa ugcaaaaaau aauaacuuua caagcucaaa ucuuguaaug 120gcugcuuaag uuagcagagg guuuuguuga auuuggcuuu gagguucacu uauacucuuu 180ucgacaucaa agcuugcuua aaaauguuuu caaguugauu uuuagggacu uuuauacuug 240agagcaauuu ggugguuugc uaguauuucc aaaccauauu gcuuaauaaa auacuagaua 300agcuuguaga agcuuauagu auuauuuuua ggacgcgggu ucaauucccg ccaucuccac 360ca                                                                362<210> 17<211> 359<212> DNA<213> Campylobacter jejuni<400> 17gggagcgact tggcttcgac aggagtaagt ctgcttagat ggcatgtcgc tttgggcaaa 60gcgtaaaaag cccaaataaa attaaacgca aacaacgtta aattcgctcc tgcttacgct 120aaagctgcgt aagttcagtt gagcctgaaa tttaagtcat actatctagc ttaattttcg 180gtcatttttg atagtgtagc cttgcgtttg acaagcgttg aggtgaaata aagtcttagc 240cttgcttttg agttttggaa gatgagcgaa gtagggtgaa gtagtcatct ttgctaagca 300tgtagaggtc tttgtgggat tatttttgga caggggttcg attcccctcg cttccacca  359<210> 18<211> 359<212> RNA<213> Campylobacter jejuni<400> 18gggagcgacu uggcuucgac aggaguaagu cugcuuagau ggcaugucgc uuugggcaaa 60gcguaaaaag cccaaauaaa auuaaacgca aacaacguua aauucgcucc ugcuuacgcu 120aaagcugcgu aaguucaguu gagccugaaa uuuaagucau acuaucuagc uuaauuuucg 180gucauuuuug auaguguagc cuugcguuug acaagcguug aggugaaaua aagucuuagc 240cuugcuuuug aguuuuggaa gaugagcgaa guagggugaa guagucaucu uugcuaagca 300uguagagguc uuugugggau uauuuuugga cagggguucg auuccccucg cuuccacca  359<210> 19<211> 420<212> DNA<213> Chlamydia trachomatis (D/UW-3/CX)<400> 19gggggtgtaa aggtttcgac ttagaaatga agcgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccgaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120cagcttcgct gatctcgaag atctaagagt agctgcttaa ttagcaaagt tgttacctaa 180atacgggtga cccggtgttc gcgagctcca ccagaggttt tcgaaacacc gtcatgtatc 240tggttagaac ttaggtcctt taattctcga ggaaatgagt ttgaaattta atgagagtcg 300ttagtctcta taggggtttc tagctgagga gacataacgt atagtaccta ggaactaagc 360atgtagaggt tagcggggag tttactaagg acgagagttc gactctctcc acctccacca 420<210> 20<211> 420<212> RNA<213> Chlamydia trachomatis (D/UW-3/CX)<400> 20ggggguguaa agguuucgac uuagaaauga agcguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccgaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120cagcuucgcu gaucucgaag aucuaagagu agcugcuuaa uuagcaaagu uguuaccuaa 180auacggguga cccgguguuc gcgagcucca ccagagguuu ucgaaacacc gucauguauc 240ugguuagaac uuagguccuu uaauucucga ggaaaugagu uugaaauuua augagagucg 300uuagucucua uagggguuuc uagcugagga gacauaacgu auaguaccua ggaacuaagc 360auguagaggu uagcggggag uuuacuaagg acgagaguuc gacucucucc accuccacca 420<210> 21<211> 421<212> DNA<213> Chlamydia trachomatis(小鼠肺炎)<400> 21gggggtgtaa aggtttcgac ttagaaatga agcgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccgaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120cagcttcgct gatcttaatg atctaagagt tgctgcttaa ttagcaaagt tgttacctaa 180gtactggtaa cccggtgttc gcgagctcca ccagaggttt tcgaaacgcc gtcatttatc 240tggttagaat tagggccttt taactctcaa gggaactaat ttgaatttta atgagagtcg 300ttggtctcta tagaggtttc tagctgagga gatataacgt aaaatattct agaaactaag 360catgtagagg ttagcgggga gtttactaag gacgagagtt cgaatctctc cacctccacc 420a                                                                 421<210> 22<211> 421<212> RNA<213> Chlamydia trachomatis(小鼠肺炎)<400> 22ggggguguaa agguuucgac uuagaaauga agcguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccgaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120cagcuucgcu gaucuuaaug aucuaagagu ugcugcuuaa uuagcaaagu uguuaccuaa 180guacugguaa cccgguguuc gcgagcucca ccagagguuu ucgaaacgcc gucauuuauc 240ugguuagaau uagggccuuu uaacucucaa gggaacuaau uugaauuuua augagagucg 300uuggucucua uagagguuuc uagcugagga gauauaacgu aaaauauucu agaaacuaag 360cauguagagg uuagcgggga guuuacuaag gacgagaguu cgaaucucuc caccuccacc 420a                                                                 421<210> 23<211> 404<212> DNA<213> Chlorobium tepidum<400> 23ggggatgaca ggctatcgac aggataggtg tgagatgtcg ttgcactccg agtttcagca 60tggacggact cgttaaacaa gtctatgtac caatagatgc agacgattat tcgtatgcaa 120tggctgcctg attagcacaa gttaattcag aagccatcgt cctgcggtga atgcgcttac 180tctgaagccg ccggatggca taacccgcgc ttgagcctac gggttcgcgc aagtaagctc 240cgtacattca tgcccgaggg ggtgtgcggg taaccaatcg ggataagggg acgaacgctg 300ctggcggtgt aatcggacca cgaaaaacca accaccagag atgagtgtgg taactgcatc 360gagcagtgtc ctggacgcgg gttcaagtcc cgccatctcc acca                  404<210> 24<211> 404<212> RNA<213> Chlorobium tepidum<400> 24ggggaugaca ggcuaucgac aggauaggug ugagaugucg uugcacuccg aguuucagca 60uggacggacu cguuaaacaa gucuauguac caauagaugc agacgauuau ucguaugcaa 120uggcugccug auuagcacaa guuaauucag aagccaucgu ccugcgguga augcgcuuac 180ucugaagccg ccggauggca uaacccgcgc uugagccuac ggguucgcgc aaguaagcuc 240cguacauuca ugcccgaggg ggugugcggg uaaccaaucg ggauaagggg acgaacgcug 300cuggcggugu aaucggacca cgaaaaacca accaccagag augagugugg uaacugcauc 360gagcaguguc cuggacgcgg guucaagucc cgccaucucc acca                  404<210> 25<211> 294<212> DNA<213> Cyanophora paradoxa(藻类)蓝色小体<400> 25ggggctgttt aggtttcgac gtttttttct aattatgttt gttaagcaag tcgaggattt 60gttctatctc gaaaatcaag aactctcaaa atttaaacgc aactaatatt gtacgtttta 120accgtaaagc agctttcgct gtttaataat tacttttaat ttaaaaacct aattttttta 180ggaatttatt tatttattgt ttatcctgct taatgaatta aaaaaagcta tacttgtgaa 240taaacgcata atttaaaaaa acggacgtgg gttcaaatcc caccagctcc acca       294<210> 26<211> 294<212> RNA<213> Cyanophora paradoxa(藻类)蓝色小体<400> 26ggggcuguuu agguuucgac guuuuuuucu aauuauguuu guuaagcaag ucgaggauuu 60guucuaucuc gaaaaucaag aacucucaaa auuuaaacgc aacuaauauu guacguuuua 120accguaaagc agcuuucgcu guuuaauaau uacuuuuaau uuaaaaaccu aauuuuuuua 180ggaauuuauu uauuuauugu uuauccugcu uaaugaauua aaaaaagcua uacuugugaa 240uaaacgcaua auuuaaaaaa acggacgugg guucaaaucc caccagcucc acca       294<210> 27<211> 189<212> DNA<213> Clostridium acetobutylicum<400> 27aatctggcgt cgagagcggg gaaacgagcc ttacaaagct ttgagtaagg aacggaattt 60atgaagctac tgaagtgaaa agcttgtttg taggcgtttc atggagggaa tgttaaaata 120caaactgcac tcggagatgc ttaatgaaac cattttcgga caggggttcg attcccctcg 180cctccacca                                                         189<210> 28<211> 189<212> RNA<213> Clostridium acetobutylicum<400> 28aaucuggcgu cgagagcggg gaaacgagcc uuacaaagcu uugaguaagg aacggaauuu 60augaagcuac ugaagugaaa agcuuguuug uaggcguuuc auggagggaa uguuaaaaua 120caaacugcac ucggagaugc uuaaugaaac cauuuucgga cagggguucg auuccccucg 180ccuccacca                                                         189<210> 29<211> 349<212> DNA<213> Deinococcus radiodurans<400> 29gggggtgacc cggtttcgac aggggaactg aaggtgatgt tgcgtgtcga ggtgccgttg 60gcctcgtaaa caaacggcaa agccatttaa ctggcaacca gaactacgct ctcgctgctt 120aagtgagatg acgaccgtgc agcccggcct ttggcgtcgc ggaagtcact aaaaaagaag 180gctagcccag gcgattctcc atagccgacg gcgaaacttt atggagctac ggcctgcgag 240aacctgccca ctggtgagcg ccggcccgac aatcaaacag tgggatacac acgtagacgc 300acgctggacg gacctttgga cggcggttcg actccgccca cctccacca             349<210> 30<211> 349<212> RNA<213> Deinococcus radiodurans<400> 30gggggugacc cgguuucgac aggggaacug aaggugaugu ugcgugucga ggugccguug 60gccucguaaa caaacggcaa agccauuuaa cuggcaacca gaacuacgcu cucgcugcuu 120aagugagaug acgaccgugc agcccggccu uuggcgucgc ggaagucacu aaaaaagaag 180gcuagcccag gcgauucucc auagccgacg gcgaaacuuu auggagcuac ggccugcgag 240aaccugccca cuggugagcg ccggcccgac aaucaaacag ugggauacac acguagacgc 300acgcuggacg gaccuuugga cggcgguucg acuccgccca ccuccacca             349<210> 31<211> 330<212> DNA<213> Desulfovibrio desulfuricans<400> 31gggactggaa ccgtagcggc aggtcgaggc gccgctggcc tcgtaaaaag cggcacaaaa 60gtaattgcca acaacgatta cgactacgct tacgctgcct aataacagcg aggcaatgac 120cgtttaacgg tcgcgccgat cagggccatg cctgataacc ctgattggcg acacttatca 180ggctggcgaa aaccggctct cgccggggtt tttcgcgagg agtttaccgg cgggattgct 240gcgttgtgcc tggtcagggg ccaacagcgc ggtgaaatac atacttgacc taaacctgta 300atgcttcgtg tggaatgttc tcggacgggg                                  330<210> 32<211> 330<212> RNA<213> Desulfovibrio desulfuricans<400> 32gggacuggaa ccguagcggc aggucgaggc gccgcuggcc ucguaaaaag cggcacaaaa 60guaauugcca acaacgauua cgacuacgcu uacgcugccu aauaacagcg aggcaaugac 120cguuuaacgg ucgcgccgau cagggccaug ccugauaacc cugauuggcg acacuuauca 180ggcuggcgaa aaccggcucu cgccgggguu uuucgcgagg aguuuaccgg cgggauugcu 240gcguugugcc uggucagggg ccaacagcgc ggugaaauac auacuugacc uaaaccugua 300augcuucgug uggaauguuc ucggacgggg                                  330<210> 33<211> 318<212> DNA<213> Dichelobacter nodosus<400> 33ctcgaggtgc atgtcgagaa tgagagaatc tcgttaaata ctttcaaaac ttatagttgc 60aaacgacgac aactacgctt tagcggctta attcccgctt tcgcttacct agatttgtct 120gtgggtttac cgtaagcgac attaacacag aatcgctggt taacgcgtcc gctgttaatc 180ggttaaatta agcggaatcg cttgtaaaat gcctgagcgt tggctgttta tgagttaaac 240ctaattaact gctctaaaca tgtagtacca aaagttaagg attcgcggac gggggttcaa 300atccccccgc ctccacca                                               318<210> 34<211> 318<212> RNA<213> Dichelobacter nodosus<400> 34cucgaggugc augucgagaa ugagagaauc ucguuaaaua cuuucaaaac uuauaguugc 60aaacgacgac aacuacgcuu uagcggcuua auucccgcuu ucgcuuaccu agauuugucu 120guggguuuac cguaagcgac auuaacacag aaucgcuggu uaacgcgucc gcuguuaauc 180gguuaaauua agcggaaucg cuuguaaaau gccugagcgu uggcuguuua ugaguuaaac 240cuaauuaacu gcucuaaaca uguaguacca aaaguuaagg auucgcggac ggggguucaa 300auccccccgc cuccacca                                               318<210> 35<211> 367<212> DNA<213> Enterococcus faecalis<400> 35gggggcgtta cggattcgac aggcatagtt gagcttgaat tgcgtttcgt aggttacggc 60tacgttaaaa cgttacagtt aaatataact gctaaaaacg aaaacaattc tttcgcttta 120gctgcctaaa aaccagctag cgaagatcct cccggcatcg cccatgtgct cgggtcaggg 180tcctaatcga agtgggatac gctaaatttt tccgtctgta aaatttagag gagcttacca 240gactagcaat acagaatgcc tgtcactcgg cacgctgtaa agcgaacctt taaatgagtg 300tctatgaacg tagagattta agtggcaata tgtttggacg cgggttcgac tcccgccgtc 360tccacca                                                           367<210> 36<211> 367<212> RNA<213> Enterococcus faecalis<400> 36gggggcguua cggauucgac aggcauaguu gagcuugaau ugcguuucgu agguuacggc 60uacguuaaaa cguuacaguu aaauauaacu gcuaaaaacg aaaacaauuc uuucgcuuua 120gcugccuaaa aaccagcuag cgaagauccu cccggcaucg cccaugugcu cgggucaggg 180uccuaaucga agugggauac gcuaaauuuu uccgucugua aaauuuagag gagcuuacca 240gacuagcaau acagaaugcc ugucacucgg cacgcuguaa agcgaaccuu uaaaugagug 300ucuaugaacg uagagauuua aguggcaaua uguuuggacg cggguucgac ucccgccguc 360uccacca                                                           367<210> 37<211> 363<212> DNA<213> Escherichia coli<400> 37ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt 120aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag 180gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat 240caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact 300aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca 360cca                                                               363<210> 38<211> 363<212> RNA<213> Escherichia coli<400> 38ggggcugauu cuggauucga cgggauuugc gaaacccaag gugcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aaaauagucg caaacgacga aaacuacgcu uuagcagcuu 120aauaaccugc uuagagcccu cucucccuag ccuccgcucu uaggacgggg aucaagagag 180gucaaaccca aaagagaucg cguggaagcc cugccugggg uugaagcguu aaaacuuaau 240caggcuaguu uguuaguggc guguccgucc gcagcuggca agcgaaugua aagacugacu 300aagcauguag uaccgaggau guaggaauuu cggacgcggg uucaacuccc gccagcucca 360cca                                                               363<210> 39<211> 366<212> DNA<213> Haemophilus influenzae<400> 39ggggctgatt ctggattcga cgggattagc gaagcccaag gtgcacgtcg aggtgcggta 60ggcctcgtaa ataaaccgca aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc 120ttaataacct gcatttagcc ttcgcgctcc agcttccgct cgtaagacgg ggataacgcg 180gagtcaaacc aaaacgagat cgtgtggaag ccaccgtttg aggatcgaag cactaaattg 240aatcaaacta gcttaagttt agcgtgtctg tccgcatgct taagtgaaat taaagacgag 300actaaacgtg tagtactgaa ggtagagtaa tttcggacgg gggttcaact ccccccagct 360ccacca                                                            366<210> 40<211> 366<212> RNA<213> Haemophilus influenzae<400> 40ggggcugauu cuggauucga cgggauuagc gaagcccaag gugcacgucg aggugcggua 60ggccucguaa auaaaccgca aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc 120uuaauaaccu gcauuuagcc uucgcgcucc agcuuccgcu cguaagacgg ggauaacgcg 180gagucaaacc aaaacgagau cguguggaag ccaccguuug aggaucgaag cacuaaauug 240aaucaaacua gcuuaaguuu agcgugucug uccgcaugcu uaagugaaau uaaagacgag 300acuaaacgug uaguacugaa gguagaguaa uuucggacgg ggguucaacu ccccccagcu 360ccacca                                                            366<210> 41<211> 340<212> DNA<213> Helicobacter pylori (ATC 43504)<400> 41agatttcttg tcgcgcagat agcatgccaa gcgctgcttg taaaacagca acaaaaataa 60ctgtaaacaa cacagattac gctccagctt acgctaaagc tgcgtgagtt aatctccttt 120tggagctgga ctgattagaa tttctagcgt tttaatcgct ccataacctt aagctagacg 180cttttaaaag gtggttcgcc ttttaaacta agaaacaaga actcttgaaa ctatcttaag 240gttttagaaa gttggaccag agctagtttt aaggctaaaa actaaccaat tttctaagca 300ttgtagaagt ttgtgtttag ggcaagattt ttggactggg                       340<210> 42<211> 340<212> RNA<213> Helicobacter pylori (ATC 43504)<400> 42agauuucuug ucgcgcagau agcaugccaa gcgcugcuug uaaaacagca acaaaaauaa 60cuguaaacaa cacagauuac gcuccagcuu acgcuaaagc ugcgugaguu aaucuccuuu 120uggagcugga cugauuagaa uuucuagcgu uuuaaucgcu ccauaaccuu aagcuagacg 180cuuuuaaaag gugguucgcc uuuuaaacua agaaacaaga acucuugaaa cuaucuuaag 240guuuuagaaa guuggaccag agcuaguuuu aaggcuaaaa acuaaccaau uuucuaagca 300uuguagaagu uuguguuuag ggcaagauuu uuggacuggg                       340<210> 43<211> 386<212> DNA<213> Helicobacter pylori(菌株 26695)<400> 43ggggctgact tggatttcga cagatttctt gtcgcacaga tagcatgcca agcgctgctt 60gtaaaacagc aacaaaaata actgtaaaca acacagatta cgctccagct tacgctaaag 120ctgcgtgagt taatctcctt ttggagctgg actgattaga atttctagcg ttttaatcgc 180tccataacct taagctagac gcttttaaaa ggtggttcgc cttttaaact aagaaacaag 240aactcttgaa actatctcaa ggttttagaa agttggacca gagctagttt taaggctaaa 300aaaccaacca attttctaag cattgtagaa gtttgtgttt agggcaagat ttttggactg 360gggttcgatt ccccacagct ccacca                                      386<210> 44<211> 386<212> RNA<213> Helicobacter pylori (菌株 26695)<400> 44ggggcugacu uggauuucga cagauuucuu gucgcacaga uagcaugcca agcgcugcuu 60guaaaacagc aacaaaaaua acuguaaaca acacagauua cgcuccagcu uacgcuaaag 120cugcgugagu uaaucuccuu uuggagcugg acugauuaga auuucuagcg uuuuaaucgc 180uccauaaccu uaagcuagac gcuuuuaaaa ggugguucgc cuuuuaaacu aagaaacaag 240aacucuugaa acuaucucaa gguuuuagaa aguuggacca gagcuaguuu uaaggcuaaa 300aaaccaacca auuuucuaag cauuguagaa guuuguguuu agggcaagau uuuuggacug 360ggguucgauu ccccacagcu ccacca                                      386<210> 45<211> 312<212> DNA<213> Klebsiella aerogenes (NCTC 9528)<400> 45gggattcgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa 60aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc taagagccct 120ctctccctag cttccgctcc taagacgggg aataaagaga ggtcaaaccc aaaagagatc 180gcgtggaagc cctgcctggg gttgaagcgt taaaactaat caggctagtt tgtcagtggc 240gtgtccgtcc gcagctggcc agcgaatgta aagactggac taagcatgta gtgccgagga 300tgtaggaatt tc                                                     312<210> 46<211> 312<212> RNA<213> Klebsiella aerogenes (NCTC 9528)<400> 46gggauucgcg aaacccaagg ugcaugccga ggggcgguug gccucguaaa aagccgcaaa 60aaaauagucg caaacgacga aaacuacgcu uuagcagcuu aauaaccugc uaagagcccu 120cucucccuag cuuccgcucc uaagacgggg aauaaagaga ggucaaaccc aaaagagauc 180gcguggaagc ccugccuggg guugaagcgu uaaaacuaau caggcuaguu ugucaguggc 240guguccgucc gcagcuggcc agcgaaugua aagacuggac uaagcaugua gugccgagga 300uguaggaauu uc                                                     312<210> 47<211> 316<212> DNA<213> Lactobacillus lactis (NCTC 662)<400> 47aagcacagtt cgagcttgaa ttgcgtttcg taggttacgt ctacgttaaa acgttacagt 60taaatataac tgctaaaaac gaaaacaact cttacgcttt agctgcctaa aaacagttag 120cgtagatcct ctcggcatcg cccatgtgct cgagtaaggg tctcaaattt agtgggatac 180gttaaacttt tccgtctgta aagtttaaaa gagatcatca gactagcgat acagaatgcc 240tgtcactcgg caagctgtaa agcgaaacct caaatgagtt gactatgaac gtagattttt 300aagtgtcgat gtgttt                                                 316<210> 48<211> 316<212> RNA<213> Lactobacillus lactis (NCTC 662)<400> 48aagcacaguu cgagcuugaa uugcguuucg uagguuacgu cuacguuaaa acguuacagu 60uaaauauaac ugcuaaaaac gaaaacaacu cuuacgcuuu agcugccuaa aaacaguuag 120cguagauccu cucggcaucg cccaugugcu cgaguaaggg ucucaaauuu agugggauac 180guuaaacuuu uccgucugua aaguuuaaaa gagaucauca gacuagcgau acagaaugcc 240ugucacucgg caagcuguaa agcgaaaccu caaaugaguu gacuaugaac guagauuuuu 300aagugucgau guguuu                                                 316<210> 49<211> 362<212> DNA<213> Legionella pneumophila<400> 49gtgggttgca aaaccggaag tgcatgccga gaaggagatc tctcgtaaat aagactcaat 60taaatataaa tgcaaacgat gaaaactttg ctggtgggga agctatcgct gcctaataag 120cactttagtt aaaccatcac tgtgtactgg ccaataaacc cagtatcccg ttcgaccgag 180cccgcttatc ggtatcgaat caacggtcat aagagataag ctagcgtcct aatctatccc 240gggttatggc gcgaaactca gggaatcgct gtgtatcatc ctgcccgtcg gaggagccac 300agttaaattc aaaagacaag gctatgcatg tagagctaaa ggcagaggac ttgcggacgc 360gg                                                                362<210> 50<211> 362<212> RNA<213> Legionella pneumophila<400> 50guggguugca aaaccggaag ugcaugccga gaaggagauc ucucguaaau aagacucaau 60uaaauauaaa ugcaaacgau gaaaacuuug cuggugggga agcuaucgcu gccuaauaag 120cacuuuaguu aaaccaucac uguguacugg ccaauaaacc caguaucccg uucgaccgag 180cccgcuuauc gguaucgaau caacggucau aagagauaag cuagcguccu aaucuauccc 240ggguuauggc gcgaaacuca gggaaucgcu guguaucauc cugcccgucg gaggagccac 300aguuaaauuc aaaagacaag gcuaugcaug uagagcuaaa ggcagaggac uugcggacgc 360gg                                                                362<210> 51<211> 322<212> DNA<213> Listeria grayi<400> 51acagggatag gtcgagcttg agttgcgagc cggggggatc ggcccgtcat caacgtcaaa 60gccaataata actggcaaac aaaacaacaa tttagctttc gctgcctaat agcagtctga 120atagctgatc ctccgtgcat cacccatgtg ctacggtaag ggtctcactt ttaagtgggt 180tacgctggct tatctccgtc tggggcaaac gagaagagca taatcagact agctagatag 240agccctgacg ccgggcagac atctatgcga aatccaaata cggcaactac gctcgtagat 300gctcaagtgc cgatatttct gg                                          322<210> 52<211> 322<212> RNA<213> Listeria grayi<400> 52acagggauag gucgagcuug aguugcgagc cggggggauc ggcccgucau caacgucaaa 60gccaauaaua acuggcaaac aaaacaacaa uuuagcuuuc gcugccuaau agcagucuga 120auagcugauc cuccgugcau cacccaugug cuacgguaag ggucucacuu uuaagugggu 180uacgcuggcu uaucuccguc uggggcaaac gagaagagca uaaucagacu agcuagauag 240agcccugacg ccgggcagac aucuaugcga aauccaaaua cggcaacuac gcucguagau 300gcucaagugc cgauauuucu gg                                          322<210> 53<211> 322<212> DNA<213> Listeria innocua<400> 53acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tgaggttaaa tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300attcaagtgc cgatatttct gg                                          322<210> 54<211> 322<212> RNA<213> Listeria innocua<400> 54acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugagguuaaa uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auucaagugc cgauauuucu gg                                          322<210> 55<211> 322<212> DNA<213> Listeria monocytogenes (NCTC 7973)<400> 55acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tggggttaaa tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggccgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg                                          322<210> 56<211> 322<212> RNA<213> Listeria monocytogenes (NCTC 7973)<400> 56acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugggguuaaa uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggccgau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auuuaagugc cgauauuucu gg                                          322<210> 57<211> 247<212> DNA<213> Listeria monocytogenes (NCTC 11994)<400> 57caaagccaat aataactggc aaagaaaaac aaaacctagc tttcgctgcc taataagcag 60tagcatagct gatcctccgt gcatcgccca tgtgctacgg taagggtctc actctaagtg 120ggctacacta gttaatctcc gtctggggtt aaatagaaga gcttaatcag actagctgaa 180tggaagcctg ttaccgggcc gatgtttatg cgaaatgcta atacggtgac tacgctcgta 240gatattt                                                           247<210> 58<211> 247<212> RNA<213> Listeria monocytogenes (NCTC 11994)<400> 58caaagccaau aauaacuggc aaagaaaaac aaaaccuagc uuucgcugcc uaauaagcag 60uagcauagcu gauccuccgu gcaucgccca ugugcuacgg uaagggucuc acucuaagug 120ggcuacacua guuaaucucc gucugggguu aaauagaaga gcuuaaucag acuagcugaa 180uggaagccug uuaccgggcc gauguuuaug cgaaaugcua auacggugac uacgcucgua 240gauauuu                                                           247<210> 59<211> 322<212> DNA<213> Listeria murrayi<400> 59acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tgaggttaaa tagaagagct taatgagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300attcaagtgc cgatatttct gg                                          322<210> 60<211> 322<212> RNA<213> Listeria murrayi<400> 60acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugagguuaaa uagaagagcu uaaugagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auucaagugc cgauauuucu gg                                          322<210> 61<211> 322<212> DNA<213> Listeria welshimeri<400> 61acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactggct aatctccgtc tgaggttagt tggaagagct taatcagact agctgaatgg 240aagcctgtta ccgggccgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg                                          322<210> 62<211> 322<212> RNA<213> Listeria welshimeri<400> 62acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuggcu aaucuccguc ugagguuagu uggaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggccgau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auuuaagugc cgauauuucu gg                                          322<210> 63<211> 322<212> DNA<213> Marinobacter hydrocarbonoclasticus<400> 63gccggtgacg aacccttggg tgcatgccga gatggcagcg aatctcgtaa atccaaagct 60gcaacgtaat agtcgcaaac gacgaaaact acgcactggc ggcgtaagcc gttccagtcg 120tcctggctga ggcgcctata actcagtagc aacatcccag gacgtcatcg cttataggct 180gctccgttca ccagagctca ctggtgttcg gctaagatta aagagctcgc ctcttgcacc 240ctgaccttcg ggtcgcttga ggttaaatca atagaaggac actaagcatg tagacctcaa 300ggcctagtgc tggcggacgc gg                                          322<210> 64<211> 322<212> RNA<213> Marinobacter hydrocarbonoclasticus<400> 64gccggugacg aacccuuggg ugcaugccga gauggcagcg aaucucguaa auccaaagcu 60gcaacguaau agucgcaaac gacgaaaacu acgcacuggc ggcguaagcc guuccagucg 120uccuggcuga ggcgccuaua acucaguagc aacaucccag gacgucaucg cuuauaggcu 180gcuccguuca ccagagcuca cugguguucg gcuaagauua aagagcucgc cucuugcacc 240cugaccuucg ggucgcuuga gguuaaauca auagaaggac acuaagcaug uagaccucaa 300ggccuagugc uggcggacgc gg                                          322<210> 65<211> 338<212> DNA<213> Mycobacterium avium<400> 65ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa ctgaccaccg taagcgtcgt 60tgcaaataga taagcgccga ttcacatcag cgcgacttac ctctcgctgc ctaagcgaca 120gctagtccgt cagcccggga acgccctcga cccggagcct ggcgtcagct agagggatcc 180accgatgagt tcggtcgcgg gactcatcgg gacaccaaca gcgactggga tcgtcatcct 240ggcttgttcg cgtgaccagg agatccgagt agaggcatag cgaactgcgc acggagaagc 300cttgagggaa tgccgtagaa cccgggttcg attcccaa                         338<210> 66<211> 338<212> RNA<213> Mycobacterium avium<400> 66uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa cugaccaccg uaagcgucgu 60ugcaaauaga uaagcgccga uucacaucag cgcgacuuac cucucgcugc cuaagcgaca 120gcuaguccgu cagcccggga acgcccucga cccggagccu ggcgucagcu agagggaucc 180accgaugagu ucggucgcgg gacucaucgg gacaccaaca gcgacuggga ucgucauccu 240ggcuuguucg cgugaccagg agauccgagu agaggcauag cgaacugcgc acggagaagc 300cuugagggaa ugccguagaa cccggguucg auucccaa                         338<210> 67<211> 318<212> DNA<213> Mycobacterium bovis<400> 67ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa gagaccaccg taagcgtcgt 60tgcgaccaaa taagcgccga ttcacatcag cgcgactacg tctcgctgcc taagcgacgg 120ctagtctgtc agaccgggaa cgccctcggc ccggaccctg gcatcagcta gagggatcca 180ccgatgagtc cggtcgcggg actcctcggg acaaccacag cgactgggat cgtcatctcg 240gctagttcgc gtgaccggga gatccgagca gaggcatagc gaactgcgca cggagaagcc 300ttgagggaat gccgtagg                                               318<210> 68<211> 318<212> RNA<213> Mycobacterium bovis<400> 68uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa gagaccaccg uaagcgucgu 60ugcgaccaaa uaagcgccga uucacaucag cgcgacuacg ucucgcugcc uaagcgacgg 120cuagucuguc agaccgggaa cgcccucggc ccggacccug gcaucagcua gagggaucca 180ccgaugaguc cggucgcggg acuccucggg acaaccacag cgacugggau cgucaucucg 240gcuaguucgc gugaccggga gauccgagca gaggcauagc gaacugcgca cggagaagcc 300uugagggaau gccguagg                                               318<210> 69<211> 369<212> DNA<213> Mycobacterium leprae<400> 69ggggctgaaa ggtttcgact tcgcgcatcg aatcaaggga agcgtgccgg tgcaggcaag 60agaccaccgt aagcgtcgtt gcagcaatat aagcgccgat tcatatcagc gcgactatgc 120tctcgctgcc taagcgatgg ctagtctgtc agaccgggaa cgccctcgtc ccggagcctg 180gcatcagcta gagggatcta ccgatgggtt cggtcgcggg actcgtcggg acaccaaccg 240cgactgggat cgtcatcctg gctagttcgc gtgatcagga gatccgagta gaggcatagc 300gaactacgca cggagaagcc ttgagggaaa tgccgtagga cccgggttcg attcccggca 360gctccacca                                                         369<210> 70<211> 369<212> RNA<213> Mycobacterium leprae<400> 70ggggcugaaa gguuucgacu ucgcgcaucg aaucaaggga agcgugccgg ugcaggcaag 60agaccaccgu aagcgucguu gcagcaauau aagcgccgau ucauaucagc gcgacuaugc 120ucucgcugcc uaagcgaugg cuagucuguc agaccgggaa cgcccucguc ccggagccug 180gcaucagcua gagggaucua ccgauggguu cggucgcggg acucgucggg acaccaaccg 240cgacugggau cgucauccug gcuaguucgc gugaucagga gauccgagua gaggcauagc 300gaacuacgca cggagaagcc uugagggaaa ugccguagga cccggguucg auucccggca 360gcuccacca                                                         369<210> 71<211> 338<212> DNA<213> Mycobacterium paratuberculosis<400> 71ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa ctgaccaccg taagcgtcgt 60tgcaaataga taagcgccga ttcacatcag cgcgacttac ctctcgctgc ctaagcgaca 120gctagtccgt cagcccggga acgccctcga cccggagcct ggcgtcagct agagggatcc 180accgatgagt tcggtcgcgg gactcatcgg gacaccaaca gcgactggga tcgtcatcct 240ggcttgttcg cgtgaccagg agatccgagt agaggcatag cgaactgcgc acggagaagc 300cttgagggaa tgccgtagaa cccgggttcg attcccaa                         338<210> 72<211> 338<212> RNA<213> Mycobacterium paratuberculosis<400> 72uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa cugaccaccg uaagcgucgu 60ugcaaauaga uaagcgccga uucacaucag cgcgacuuac cucucgcugc cuaagcgaca 120gcuaguccgu cagcccggga acgcccucga cccggagccu ggcgucagcu agagggaucc 180accgaugagu ucggucgcgg gacucaucgg gacaccaaca gcgacuggga ucgucauccu 240ggcuuguucg cgugaccagg agauccgagu agaggcauag cgaacugcgc acggagaagc 300cuugagggaa ugccguagaa cccggguucg auucccaa                         338<210> 73<211> 368<212> DNA<213> Mycobacterium tuberculosis<400> 73ggggctgaac ggtttcgact tcgcgcatcg aatcaaggga agcgtgccgg tgcaggcaag 60agaccaccgt aagcgtcgtt gcgaccaaat aagcgccgat tcacatcagc gcgactacgc 120tctcgctgcc taagcgacgg ctagtctgtc agaccgggaa cgccctcggc ccggaccctg 180gcatcagcta gagggatcca ccgatgagtc cggtcgcggg actcctcggg acaaccacag 240cgactgggat cgtcatctcg gctagttcgc gtgaccggga gatccgagca gaggcatagc 300gaactgcgca cggagaagcc ttgagggaat gccgtaggac ccgggttcga ttcccggcag 360ctccacca                                                          368<210> 74<211> 368<212> RNA<213> Mycobacterium tuberculosis<400> 74ggggcugaac gguuucgacu ucgcgcaucg aaucaaggga agcgugccgg ugcaggcaag 60agaccaccgu aagcgucguu gcgaccaaau aagcgccgau ucacaucagc gcgacuacgc 120ucucgcugcc uaagcgacgg cuagucuguc agaccgggaa cgcccucggc ccggacccug 180gcaucagcua gagggaucca ccgaugaguc cggucgcggg acuccucggg acaaccacag 240cgacugggau cgucaucucg gcuaguucgc gugaccggga gauccgagca gaggcauagc 300gaacugcgca cggagaagcc uugagggaau gccguaggac ccggguucga uucccggcag 360cuccacca                                                          368<210> 75<211> 411<212> DNA<213> Mycoplasma capricolum<400> 75ggggatgtca tggatttgac aggatatctt tagtacatat aagcagtagt gttgtagact 60ataaatacta ctaggtttaa aaaaacgcaa ataaaaacga agaaactttt gaaatgccag 120catttatgat gaataatgca tcagctggag caaactttat gtttgcttaa taactactag 180tttagttata gtatttcacg aattatagat attttaagct ttatttataa ccgtattacc 240caagcttaat agaatatatg attgcaataa atatatttga aatctaattg caaatgatat 300ttaaccttta gttaatttta gttaaatatt ttaattagaa aattaactaa actgtagaaa 360gtatgtatta atatatcttg gacgcgagtt cgattctcgc catctccacc a          411<210> 76<211> 411<212> RNA<213> Mycoplasma capricolum<400> 76ggggauguca uggauuugac aggauaucuu uaguacauau aagcaguagu guuguagacu 60auaaauacua cuagguuuaa aaaaacgcaa auaaaaacga agaaacuuuu gaaaugccag 120cauuuaugau gaauaaugca ucagcuggag caaacuuuau guuugcuuaa uaacuacuag 180uuuaguuaua guauuucacg aauuauagau auuuuaagcu uuauuuauaa ccguauuacc 240caagcuuaau agaauauaug auugcaauaa auauauuuga aaucuaauug caaaugauau 300uuaaccuuua guuaauuuua guuaaauauu uuaauuagaa aauuaacuaa acuguagaaa 360guauguauua auauaucuug gacgcgaguu cgauucucgc caucuccacc a          411<210> 77<211> 388<212> DNA<213> Mycoplasma genitalium (ATTC 33530，#1)<400> 77ggggatgttt tgggtttgac ataatgctga tagacaaaca gtagcattgg ggtatgcccc 60ttacagcgct aggttcaata accgacaaag aaaataacga agtgttggta gaaccaaatt 120tgatcattaa ccaacaagca agtgttaact ttgcttttgc ataagtagat actaaagcta 180cagctggtga atagtcatag tttgctagct gtcatagttt atgactcgag gttaaatcgt 240tcaatttaac ctttaaaaat agaacttgtt gtttccatga ttgttttgtg atcaattgga 300aacaagacaa aaatccacaa aactaaaatg tagaagctgt ttgttgtgtc ctttatggaa 360acgggttcga ttcccgtcat ctccacca                                    388<210> 78<211> 388<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#1)<400> 78ggggauguuu uggguuugac auaaugcuga uagacaaaca guagcauugg gguaugcccc 60uuacagcgcu agguucaaua accgacaaag aaaauaacga aguguuggua gaaccaaauu 120ugaucauuaa ccaacaagca aguguuaacu uugcuuuugc auaaguagau acuaaagcua 180cagcugguga auagucauag uuugcuagcu gucauaguuu augacucgag guuaaaucgu 240ucaauuuaac cuuuaaaaau agaacuuguu guuuccauga uuguuuugug aucaauugga 300aacaagacaa aaauccacaa aacuaaaaug uagaagcugu uuguuguguc cuuuauggaa 360acggguucga uucccgucau cuccacca                                    388<210> 79<211> 243<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#2)<400> 79acaaagcgaa gacaaacaga gcaggggagc cccacagcgc aggcaaaacc gacaaagaaa 60aaacgaaggg gagaccaaag acaaaccaac aagcaaggaa cgcgcaaaga gaacaaagca 120cagcgggaaa gcaaggcagc gcaagagacc gaggaaacgc aaaaccaaaa aagaacggcc 180agagggacaa ggaaacaaga caaaaaccac aaaacaaaag agaagcgggg ccaggaaacg 240ggc                                                               243<210> 80<211> 350<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#2)<400> 80acauaaugcu gauagacaaa caguagcauu gggguaugcc ccuuacagcg cuagguucaa 60uaaccgacaa agaaaauaac gaaguguugg uagauccaaa uuugaucauu aaccaacaag 120caaguguuaa cuuugcuuuu gcauaaguag auacuaaagc uacagcuggu gaauagucau 180aguuugcuag cugucauagu uuaugacucg agguuaaauc guucaauuua accuuuaaaa 240auagaacuug uuguuuccau gauuguuuug ugaucaauug gaaacaagac aaaaauccac 300aaaacuaaaa uguagaagcu guuuguugug uccuuuaugg aaacggguuc            350<210> 81<211> 387<212> DNA<213> Mycoplasma pneumophila<400> 81ggggatgtag aggttttgac ataatgttga aaggaaaaca gttgcagtgg ggtatgcccc 60ttacagctct aggtataata accgacaaaa ataacgacga agttttggta gatccaatgt 120tgatcgctaa ccaacaagca agtatcaact acgctttcgc ttagaacata ctaaagctac 180acgaattgaa tcgccatagt ttggttcgtg tcacagttta tggctcgggg ttaactggtt 240caacttaatc cttaaattat gaacttatcg tttacttgtt tgtcttatga tctaaagtaa 300gcgagacatt aaaacataag actaaactgt agaagctgtt ttaccaatcc tttatggaaa 360cgggttcgat tcccgtcatc tccacca                                     387<210> 82<211> 387<212> RNA<213> Mycoplasma pneumophila<400> 82ggggauguag agguuuugac auaauguuga aaggaaaaca guugcagugg gguaugcccc 60uuacagcucu agguauaaua accgacaaaa auaacgacga aguuuuggua gauccaaugu 120ugaucgcuaa ccaacaagca aguaucaacu acgcuuucgc uuagaacaua cuaaagcuac 180acgaauugaa ucgccauagu uugguucgug ucacaguuua uggcucgggg uuaacugguu 240caacuuaauc cuuaaauuau gaacuuaucg uuuacuuguu ugucuuauga ucuaaaguaa 300gcgagacauu aaaacauaag acuaaacugu agaagcuguu uuaccaaucc uuuauggaaa 360cggguucgau ucccgucauc uccacca                                     387<210> 83<211> 318<212> DNA<213> Neisseria gonorrhoeae (ATCC 19424)<400> 83gggggttgcg aagcagatgc gggcataccg gggtctcaga ttcccgtaaa acactgaatt 60caaatagtcg caaacgacga aacttacgct ttagccgctt aaggctagcc gttgcagcag 120tcggtcaatg ggctgtgtgg cgaaagccac cgcaacgtca tcttacattg actggtttcc 180agccgggtta cttggcagga aataagactt aaggtaactg gtttccaaaa ggcctgttgg 240tcggcatgat ggaaataaga ttttcaaata gacacaacta agtatgtaga acgctttgta 300gaggactttc ggacgggg                                               318<210> 84<211> 318<212> RNA<213> Neisseria gonorrhoeae (ATCC 19424)<400> 84ggggguugcg aagcagaugc gggcauaccg gggucucaga uucccguaaa acacugaauu 60caaauagucg caaacgacga aacuuacgcu uuagccgcuu aaggcuagcc guugcagcag 120ucggucaaug ggcugugugg cgaaagccac cgcaacguca ucuuacauug acugguuucc 180agccggguua cuuggcagga aauaagacuu aagguaacug guuuccaaaa ggccuguugg 240ucggcaugau ggaaauaaga uuuucaaaua gacacaacua aguauguaga acgcuuugua 300gaggacuuuc ggacgggg                                               318<210> 85<211> 363<212> DNA<213> Neisseria gonorrhoeae (FA 1090)<400> 85gggggcgacc ttggtttcga cgggggttgc gaagcagatg cgggcatacc ggggtctcag 60attcccgtaa aacactgaat tcaaatagtc gcaaacgacg aaacttacgc tttagccgct 120taaggctagc cgttgcagca gtcggtcaat gggctgtgtg gtgaaagcca ccgcaacgtc 180atcttacatt gactggtttc cagccgggtt acttggcagg aaataagact taaggtaact 240ggtttccaaa aggcctgttg gtcggcatga tggaaataag attttcaaat agacacaact 300aagtatgtag aacgctttgt agaggacttt cggacggggg ttcgattccc cccgcctcca 360cca                                                               363<210> 86<211> 363<212> RNA<213> Neisseria gonorrhoeae (FA 1090)<400> 86gggggcgacc uugguuucga cggggguugc gaagcagaug cgggcauacc ggggucucag 60auucccguaa aacacugaau ucaaauaguc gcaaacgacg aaacuuacgc uuuagccgcu 120uaaggcuagc cguugcagca gucggucaau gggcugugug gugaaagcca ccgcaacguc 180aucuuacauu gacugguuuc cagccggguu acuuggcagg aaauaagacu uaagguaacu 240gguuuccaaa aggccuguug gucggcauga uggaaauaag auuuucaaau agacacaacu 300aaguauguag aacgcuuugu agaggacuuu cggacggggg uucgauuccc cccgccucca 360cca                                                               363<210> 87<211> 363<212> DNA<213> Neisseria meningitidis<400> 87gggggcgacc ttggtttcga cgggggttgc gaagcagatg cgggcataccggggtctcag  60attcccgtaa aacactgaat tcaaatagtc gcaaacgacg aaacttacgc tttagccgct 120taaggctagc cgttgcagca gtcggtcaat gggctgtgtg gcgaaagcca ccgcaacgtc 180atcttacatt gactggtttc ctgccgggtt atttggcagg aaatgagatt taaggtaact 240ggtttccaaa aggcctgttg gtcggcatga tggaaataag attttcaaat agacacaact 300aagtatgtag aacgctttgt agaggacttt cggacggggg ttcgattccc cccgcctcca 360cca                                                               363<210> 88<211> 363<212> RNA<213> Neisseria meningitidis<400> 88gggggcgacc uugguuucga cggggguugc gaagcagaug cgggcauacc ggggucucag 60auucccguaa aacacugaau ucaaauaguc gcaaacgacg aaacuuacgc uuuagccgcu 120uaaggcuagc cguugcagca gucggucaau gggcugugug gcgaaagcca ccgcaacguc 180aucuuacauu gacugguuuc cugccggguu auuuggcagg aaaugagauu uaagguaacu 240gguuuccaaa aggccuguug gucggcauga uggaaauaag auuuucaaau agacacaacu 300aaguauguag aacgcuuugu agaggacuuu cggacggggg uucgauuccc cccgccucca 360cca                                                               363<210> 89<211> 385<212> DNA<213> Nostoc muscorum PCC7120<400> 89gggtccgtcg gtttcgacag gttggcgaac gctactctgt gattcaggtc gagagtgagt 60ctcctctgca aatcaaggct caaaacaaaa gtaaatgcga ataacatcgt taaatttgct 120cgtaaggacg ctctagtagc tgcctaaata gcctctttca ggttcgagcg tcttcggttt 180gactccgtta aggactgaag accaaccccc aacggatgct ctagcaatgt tctctggttg 240gcttgctagc taagatttaa tcagagcatc ctacgttcgg gataatgaac gattcccgcc 300ttgagggtca gaaaggctaa acctgtgaat gagcgggggg tcaataccca atttggacag 360cagttcgact ctgctcgatc cacca                                       385<210> 90<211> 385<212> RNA<213> Nostoc muscorum PCC7120<400> 90ggguccgucg guuucgacag guuggcgaac gcuacucugu gauucagguc gagagugagu 60cuccucugca aaucaaggcu caaaacaaaa guaaaugcga auaacaucgu uaaauuugcu 120cguaaggacg cucuaguagc ugccuaaaua gccucuuuca gguucgagcg ucuucgguuu 180gacuccguua aggacugaag accaaccccc aacggaugcu cuagcaaugu ucucugguug 240gcuugcuagc uaagauuuaa ucagagcauc cuacguucgg gauaaugaac gauucccgcc 300uugaggguca gaaaggcuaa accugugaau gagcgggggg ucaauaccca auuuggacag 360caguucgacu cugcucgauc cacca                                       385<210> 91<211> 371<212> DNA<213> Odontella sinensis(硅藻属)叶绿体<400> 91ggggctgact tggtttcgac atttaaaaat tgttacagta tgatgcaggt cgaagtttct 60aatcttcgta aaaaaagaga aatttataat aaatgctaat aatttaattt cttctgtgtt 120taaaagttta tcaactaagc aaaatagttt aaatttaagt tttgctgttt aagttttatg 180cacatttaat gatctagtaa ataactttgt tcgctataat ttatatttat aactagactt 240ttgtcttttt tatagtttag aataacttta tcatttcaaa cctcgttcca tctagttgaa 300ctaaacctgt gaacgaatac tataataaaa tttttagatg gacgtgggtt cgactcccat 360cagctccacc a                                                      371<210> 92<211> 371<212> RNA<213> Odontella sinensis(硅藻属)叶绿体<400> 92ggggcugacu ugguuucgac auuuaaaaau uguuacagua ugaugcaggu cgaaguuucu 60aaucuucgua aaaaaagaga aauuuauaau aaaugcuaau aauuuaauuu cuucuguguu 120uaaaaguuua ucaacuaagc aaaauaguuu aaauuuaagu uuugcuguuu aaguuuuaug 180cacauuuaau gaucuaguaa auaacuuugu ucgcuauaau uuauauuuau aacuagacuu 240uugucuuuuu uauaguuuag aauaacuuua ucauuucaaa ccucguucca ucuaguugaa 300cuaaaccugu gaacgaauac uauaauaaaa uuuuuagaug gacguggguu cgacucccau 360cagcuccacc a                                                      371<210> 93<211> 323<212> DNA<213>Porphyra purpureum(红藻)叶绿体<400> 93ggggctgcaa ggtttctaca ttgtgaaaaa acaaatatat gaaagtaaaa cgagctcatt 60attagagctt ttagttaaat aaatgcagaa aataatatta ttgctttttc tcgaaaatta 120gctgttgcat aaatagtctc aatttttgta attcgaagtg atagactctt atacactacg 180aatattctgt tagagttgct cttaataaaa gaaaagtaaa aaaatacaaa ttcttatgtt 240ttttacctga attgattcaa tttaaggtta gtattttttg atttttacaa tggacgtggg 300ttcaagtccc accagctcca cca                                         323<210> 94<211> 323<212> RNA<213>Porphyra purpureum(红藻)叶绿体<400> 94ggggcugcaa gguuucuaca uugugaaaaa acaaauauau gaaaguaaaa cgagcucauu 60auuagagcuu uuaguuaaau aaaugcagaa aauaauauua uugcuuuuuc ucgaaaauua 120gcuguugcau aaauagucuc aauuuuugua auucgaagug auagacucuu auacacuacg 180aauauucugu uagaguugcu cuuaauaaaa gaaaaguaaa aaaauacaaa uucuuauguu 240uuuuaccuga auugauucaa uuuaagguua guauuuuuug auuuuuacaa uggacguggg 300uucaaguccc accagcucca cca                                         323<210> 95<211> 407<212> DNA<213> Porphyromonas gingivalis<400> 95ggggctgacc ggctttgaca gcgtgatgaa gcggtatgta agcatgtagt gcgtgggtgg 60cttgcactat aatctcagac atcaaaagtt taattggcga aaataactac gctctcgctg 120cgtaatcgaa gaatagtaga ttagacgctt catcgccgcc aaagtggcag cgacgagaca 180tcgcccgagc agctttttcc cgaagtagct cgatggtgcg gtgctgacaa atcgggaacc 240gctacaggat gcttcctgcc tgtggtcaga tcgaacggaa gataaggatc gtgcattggg 300tcgtttcagc ctccgctcgc tcacgaaaat tccaactgaa actaaacatg tagaaagcat 360attgattcca tgtttggacg agggttcaat tccctccagc tccacca               407<210> 96<211> 407<212> RNA<213> Porphyromonas gingivalis<400> 96ggggcugacc ggcuuugaca gcgugaugaa gcgguaugua agcauguagu gcgugggugg 60cuugcacuau aaucucagac aucaaaaguu uaauuggcga aaauaacuac gcucucgcug 120cguaaucgaa gaauaguaga uuagacgcuu caucgccgcc aaaguggcag cgacgagaca 180ucgcccgagc agcuuuuucc cgaaguagcu cgauggugcg gugcugacaa aucgggaacc 240gcuacaggau gcuuccugcc uguggucaga ucgaacggaa gauaaggauc gugcauuggg 300ucguuucagc cuccgcucgc ucacgaaaau uccaacugaa acuaaacaug uagaaagcau 360auugauucca uguuuggacg aggguucaau ucccuccagc uccacca               407<210> 97<211> 310<212> DNA<213> Proteus rettgeri (NCTC 10975)<400> 97gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa 60aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct 120ctctccctag cctccgctct tggacgggga tcaagagagg tcaaacccaa aagagatcgc 180gtggatgcct tgcctggggt tgaagcgtta aacttaatca ggatagtttg ttggtggcgt 240gtctgtccgc agctggcaaa tgaattcaaa gactagacta agcatgtagt accgaggatg 300tagaaatttc                                                        310<210> 98<211> 310<212> RNA<213> Proteus rettgeri (NCTC 10975)<400> 98gggauuugcg aaacccaagg ugcaugccga ggggcgguug gccucguaaa aagccgcaaa 60aaaauagucg caaacgacga aaacuacgcu uuagcagcuu aauaaccugc uuagagcccu 120cucucccuag ccuccgcucu uggacgggga ucaagagagg ucaaacccaa aagagaucgc 180guggaugccu ugccuggggu ugaagcguua aacuuaauca ggauaguuug uugguggcgu 240gucuguccgc agcuggcaaa ugaauucaaa gacuagacua agcauguagu accgaggaug 300uagaaauuuc                                                        310<210> 99<211> 241<212> DNA<213> Pseudoalteromonas haloplanktis<400> 99gggagcgaaa cccaagggca gccgaggggc ggggcccgaa aaagccgcaa aaaaaagcgc 60aaacgacgaa aacacgcagc agcaaaaccg cagagccccc cccagccccg ccggacgggg 120acaagagagg caaacccaaa agagacgcgg gagccgccgg gggaagcgaa acaacaggaa 180ggggggcggc gccgcagcgg caaagaacaa agacagacaa gcagagaccg aggagagaaa 240c                                                                 241<210> 100<211> 313<212> RNA<213> Pseudoalteromonas haloplanktis<400> 100ggaauucaag aagcccgagg ugcaugucga ggugcgguuu gccucguaaa aaagccgcaa 60uuuaaaguaa ucgcaaacga cgauaacuac ucucuagcag cuuaggcugg cuagcgcucc 120uuccauguau ucuuguggac uggauuuugg agugucaccc uaacaccuga ucgcgacgga 180aacccuggcc gggguugaag cguuaaaacu aagcggccuc gccuuuaucu accguguuug 240uccgggauuu aaagguuaau uaaaugacaa uacuaaacau guaguaccga cggucgaggc 300uuuucggacg ggg                                                    313<210> 101<211> 353<212> DNA<213> Pseudomonas aeruginosa<400> 101ggggccgatt aggattcgac gccggtaaca aaagttgagg ggcatgccga gttggtagca 60gaactcgtaa attcgctgct gcaaacttat agttgccaac gacgacaact acgctctagc 120tgcttaatgc ggctagcagt cgctagggga tgcctgtaaa cccgaaacga ctgtcagata 180gaacaggatc gccgccaagt tcgctgtaga cgtaacggct aaaactcata cagctcgctc 240caagcaccct gccactcggg cggcgcggag ttaactcagt agagctggct aagcatgtaa 300aaccgatagc ggaaagctgg cggacggggg ttcaaatccc cccggttcca cca        353<210> 102<211> 353<212> RNA<213> Pseudomonas aeruginosa<400> 102ggggccgauu aggauucgac gccgguaaca aaaguugagg ggcaugccga guugguagca 60gaacucguaa auucgcugcu gcaaacuuau aguugccaac gacgacaacu acgcucuagc 120ugcuuaaugc ggcuagcagu cgcuagggga ugccuguaaa cccgaaacga cugucagaua 180gaacaggauc gccgccaagu ucgcuguaga cguaacggcu aaaacucaua cagcucgcuc 240caagcacccu gccacucggg cggcgcggag uuaacucagu agagcuggcu aagcauguaa 300aaccgauagc ggaaagcugg cggacggggg uucaaauccc cccgguucca cca        353<210> 103<211> 363<212> DNA<213> Salmonella typhimurium<400> 103ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa aaaaatagtc gcaaacgacg aaacctacgc tttagcagct 120taataacctg cttagagccc tctctcccta gcctccgctc ttaggacggg gatcaagaga 180ggtcaaaccc aaaagagatc gcgcggatgc cctgcctggg gttgaagcgt taaaacgaat 240caggctagtc tggtagtggc gtgtccgtcc gcaggtgcca ggcgaatgta aagactgact 300aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca 360cca                                                               363<210> 104<211> 363<212> RNA<213> Salmonella typhimurium<400> 104ggggcugauu cuggauucga cgggauuugc gaaacccaag gugcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aaaaauaguc gcaaacgacg aaaccuacgc uuuagcagcu 120uaauaaccug cuuagagccc ucucucccua gccuccgcuc uuaggacggg gaucaagaga 180ggucaaaccc aaaagagauc gcgcggaugc ccugccuggg guugaagcgu uaaaacgaau 240caggcuaguc ugguaguggc guguccgucc gcaggugcca ggcgaaugua aagacugacu 300aagcauguag uaccgaggau guaggaauuu cggacgcggg uucaacuccc gccagcucca 360cca                                                               363<210> 105<211> 355<212> DNA<213> Shewanella putrefaciens<400> 105gggggcgatt ctggattcga caggattcac gaaaccctgg gagcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa agttatagtt gcaaacgacg ataactacgc tctagccgct 120taatgccgct agccatctac cacacgcttt gcacatgggc agtggatttg atggtcatct 180cacatcgtgc tagcgaggga accctgtctg ggggtgaacc gcgaaacagt accggactca 240ccgtgtggga tcctgtcttt cggagttcaa acggttaaac aatagaaaga ctaagcatgt 300agcgccttgg atgtaggttt tctggacgcg ggttcaagtc ccgccgcctc cacca      355<210> 106<211> 355<212> RNA<213> Shewanella putrefaciens<400> 106gggggcgauu cuggauucga caggauucac gaaacccugg gagcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aguuauaguu gcaaacgacg auaacuacgc ucuagccgcu 120uaaugccgcu agccaucuac cacacgcuuu gcacaugggc aguggauuug auggucaucu 180cacaucgugc uagcgaggga acccugucug ggggugaacc gcgaaacagu accggacuca 240ccguguggga uccugucuuu cggaguucaa acgguuaaac aauagaaaga cuaagcaugu 300agcgccuugg auguagguuu ucuggacgcg gguucaaguc ccgccgccuc cacca      355<210> 107<211> 362<212> DNA<213> Staphylococcus aureus<400> 107ggggacgttc atggattcga caggggtccc ccgagctcat taagcgtgtc ggagggttgt 60cttcgtcatc aacacacaca gtttataata actggcaaat caaacaataa tttcgcagta 120gctgcctaat cgcactctgc atcgcctaac agcatttcct atgtgctgtt aacgcgattc 180aaccttaata ggatatgcta aacactgccg tttgaagtct gtttagaaga aacttaatca 240aactagcatc atgttggttg tttatcactt ttcatgatgc gaaacctatc gataaactac 300acacgtagaa agatgtgtat caggaccttt ggacgcgggt tcaaatcccg ccgtctccac 360ca                                                                362<210> 108<211> 362<212> RNA<213> Staphylococcus aureus<400> 108ggggacguuc auggauucga cagggguccc ccgagcucau uaagcguguc ggaggguugu 60cuucgucauc aacacacaca guuuauaaua acuggcaaau caaacaauaa uuucgcagua 120gcugccuaau cgcacucugc aucgccuaac agcauuuccu augugcuguu aacgcgauuc 180aaccuuaaua ggauaugcua aacacugccg uuugaagucu guuuagaaga aacuuaauca 240aacuagcauc auguugguug uuuaucacuu uucaugaugc gaaaccuauc gauaaacuac 300acacguagaa agauguguau caggaccuuu ggacgcgggu ucaaaucccg ccgucuccac 360ca                                                                362<210> 109<211> 349<212> DNA<213> Streptococcus gordonii<400> 109ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcat ctagcggatg 60taaaacgcca gttaaatata actgcaaaaa ataatacttc ttacgcttta gctgcctaaa 120aaccagcggg cgtgacccga ttcggattgc ttgtgtctga tgacaggtct tattattagc 180aagctacggt agaatcttgt ctagtgattt tacaagagat tgatagactc gcttgatttg 240ggcttgagtt atgtgtcaaa atcaagttaa aacaatacat agcctatggt tgtagacaaa 300tgtgttggca gatgtttgga cgtgggttcg actcccaccg gctccacca             349<210> 110<211> 349<212> RNA<213> Streptococcus gordonii<400> 110ggggucguua cggauucgac aggcauuaug aggcauauuu ugcgacucau cuagcggaug 60uaaaacgcca guuaaauaua acugcaaaaa auaauacuuc uuacgcuuua gcugccuaaa 120aaccagcggg cgugacccga uucggauugc uugugucuga ugacaggucu uauuauuagc 180aagcuacggu agaaucuugu cuagugauuu uacaagagau ugauagacuc gcuugauuug 240ggcuugaguu augugucaaa aucaaguuaa aacaauacau agccuauggu uguagacaaa 300uguguuggca gauguuugga cguggguucg acucccaccg gcuccacca             349<210> 111<211> 349<212> DNA<213> Streptococcus mutans<400> 111ggggtcgtta cggattcgac aggcattatg agacctattt tgcgactcat ctagcggatg 60taaaacgcca gttaaatata actgcaaaaa atacaaattc ttacgcagta gctgcctaaa 120aaccagcctg tgtgatcaat aacaaattgc ttgtgtttgt tgattggtct tattgttaac 180aagctacgtt agaactgagt caggctgttc taaaagagtt ctactgactc gcatcgttag 240agtttgagtt atgtattgta acggtgttaa ataaacacat aacctatagt tgtagacaaa 300tgggttagca gatgtttgga cgtgggttcg actcccaccg gctccacca             349<210> 112<211> 349<212> RNA<213> Streptococcus mutans<400> 112ggggucguua cggauucgac aggcauuaug agaccuauuu ugcgacucau cuagcggaug 60uaaaacgcca guuaaauaua acugcaaaaa auacaaauuc uuacgcagua gcugccuaaa 120aaccagccug ugugaucaau aacaaauugc uuguguuugu ugauuggucu uauuguuaac 180aagcuacguu agaacugagu caggcuguuc uaaaagaguu cuacugacuc gcaucguuag 240aguuugaguu auguauugua acgguguuaa auaaacacau aaccuauagu uguagacaaa 300uggguuagca gauguuugga cguggguucg acucccaccg gcuccacca             349<210> 113<211> 348<212> DNA<213> Streptococcus pneumoniae<400> 113ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt gtggcgacgt 60aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag ctgcctaaaa 120accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt attattagcg 180agatacgatt aagccttgtc tagcggtttg ataagagatt gatagactcg cagtttctag 240acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt gtagacaaat 300atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccacca              348<210> 114<211> 348<212> RNA<213> Streptococcus pneumoniae<400> 114ggggucguua cggauucgac aggcauuaug aggcauauuu ugcgacucgu guggcgacgu 60aaacgcucag uuaaauauaa cugcaaaaaa uaacacuucu uacgcucuag cugccuaaaa 120accagcaggc gugacccgau uuggauugcu cguguucaau gacaggucuu auuauuagcg 180agauacgauu aagccuuguc uagcgguuug auaagagauu gauagacucg caguuucuag 240acuugaguua ugugucgagg ggcuguuaaa auaauacaua accuaugguu guagacaaau 300auguuggcag guguuuggac guggguucga cucccaccgg cuccacca              348<210> 115<211> 348<212> DNA<213> Streptococcus pyogenes<400> 115ggggttgtta cggattcgac aggcattatg aggcatgttt tgcgtcccat cggcagatgt 60aaattgccag ttaaatataa ctgcaaaaaa tacaaactct tacgctttag ctgcctaaaa 120accagctagc gtgacttcta caagattgct tgtgtcctgt tagaagtctc aaaatagcaa 180gctacggtta cgaaattgtc tagtttcgtg acaagagatt gatagactcg caaactaatg 240gcttgagtta tgtgtcttta gtttgttaaa tgaagacata acctatggac gtagacaaat 300atgttggcag gtgtttggac gtgggttcga ctcccaccag ctccacca              348<210> 116<211> 348<212> RNA<213> Streptococcus pyogenes<400> 116gggguuguua cggauucgac aggcauuaug aggcauguuu ugcgucccau cggcagaugu 60aaauugccag uuaaauauaa cugcaaaaaa uacaaacucu uacgcuuuag cugccuaaaa 120accagcuagc gugacuucua caagauugcu uguguccugu uagaagucuc aaaauagcaa 180gcuacgguua cgaaauuguc uaguuucgug acaagagauu gauagacucg caaacuaaug 240gcuugaguua ugugucuuua guuuguuaaa ugaagacaua accuauggac guagacaaau 300auguuggcag guguuuggac guggguucga cucccaccag cuccacca              348<210> 117<211> 394<212> DNA<213> Synechococcus sp. PCC6301<400> 117ggggctgtaa tggtttcgac gtgttggtga atccttcacc gtgattcagg ccgagaggga 60gtccactctc gtaaatccag gctcaaccaa aagtaactgc gaacaacatc gttcctttcg 120ctcgtaaggc tgctcctgta gctgcttaaa cgccacaaac tttctggctc gagcgtctag 180tcgtagactc cgttaatacg cctagactta aacccccaac ggatgctcga gtggcggcct 240caggtccgtc ctctcgctaa gcaaaaacct gagcatcccg ccaacgggga taatcgttgg 300ctcccgcaca gtgggtcaac cgtgctaagc ctgtgaacga gcggaaagtt actagtcaat 360gcggacagcg gttcgattcc gctcagctcc acca                             394<210> 118<211> 394<212> RNA<213> Synechococcus sp. PCC6301<400> 118ggggcuguaa ugguuucgac guguugguga auccuucacc gugauucagg ccgagaggga 60guccacucuc guaaauccag gcucaaccaa aaguaacugc gaacaacauc guuccuuucg 120cucguaaggc ugcuccugua gcugcuuaaa cgccacaaac uuucuggcuc gagcgucuag 180ucguagacuc cguuaauacg ccuagacuua aacccccaac ggaugcucga guggcggccu 240cagguccguc cucucgcuaa gcaaaaaccu gagcaucccg ccaacgggga uaaucguugg 300cucccgcaca gugggucaac cgugcuaagc cugugaacga gcggaaaguu acuagucaau 360gcggacagcg guucgauucc gcucagcucc acca                             394<210> 119<211> 399<212> DNA<213> Synechocystis PCC6803<400> 119ggggccgcaa tggtttcgac aggttggcga aagcttgccc gtgatacagg tcgagagtga 60gtctcctctc gcaaatcaaa ggctcaaaaa aaagtaactg cgaataacat cgtcagcttc 120aaacgggtag ccatagcagc ctagtctgta aaagctacat tttcttgtca aagaccgttt 180acttcttttc tgactccgtt aaggattaga ggttaacccc aacggatgct ttgtttggct 240cttctctagt tagctaaaca atcaagactc agactagagc atcccaccat cagggataat 300cgatggtccc cgtcctaggg ctagaaggac taaacctgtg aatgagcgga aagttaatac 360ccagtttgga cagcagttca attctgctcg gctccacca                        399<210> 120<211> 399<212> RNA<213> Synechocystis PCC6803<400> 120ggggccgcaa ugguuucgac agguuggcga aagcuugccc gugauacagg ucgagaguga 60gucuccucuc gcaaaucaaa ggcucaaaaa aaaguaacug cgaauaacau cgucagcuuc 120aaacggguag ccauagcagc cuagucugua aaagcuacau uuucuuguca aagaccguuu 180acuucuuuuc ugacuccguu aaggauuaga gguuaacccc aacggaugcu uuguuuggcu 240cuucucuagu uagcuaaaca aucaagacuc agacuagagc aucccaccau cagggauaau 300cgaugguccc cguccuaggg cuagaaggac uaaaccugug aaugagcgga aaguuaauac 360ccaguuugga cagcaguuca auucugcucg gcuccacca                        399<210> 121<211> 356<212> DNA<213> Thermotoga maritima<400> 121gggggcgaac gggttcgacg gggatggagt cccctgggaa gcgagccgag gtccccacct 60cctcgtaaaa aaggtgggac aaagaataag tgccaacgaa cctgttgctg ttgccgctta 120atagataagc ggccgtcctc tccgaagttg gctgggcttc ggaagagggc gtgagagatc 180cagcctaccg attcagcttc gccttccggc ctgaatcggg aaaactcagg aaggctgtgg 240gagaggacac cctgcccgtg ggaggtccct cccgagagcg aaaacacggg ctgcgctcgg 300agaagcccag gggcctccat cttcggacgg gggttcgaat ccccccgcct ccacca     356<210> 122<211> 356<212> RNA<213> Thermotoga maritima<400> 122gggggcgaac ggguucgacg gggauggagu ccccugggaa gcgagccgag guccccaccu 60ccucguaaaa aaggugggac aaagaauaag ugccaacgaa ccuguugcug uugccgcuua 120auagauaagc ggccguccuc uccgaaguug gcugggcuuc ggaagagggc gugagagauc 180cagccuaccg auucagcuuc gccuuccggc cugaaucggg aaaacucagg aaggcugugg 240gagaggacac ccugcccgug ggaggucccu cccgagagcg aaaacacggg cugcgcucgg 300agaagcccag gggccuccau cuucggacgg ggguucgaau ccccccgccu ccacca     356<210> 123<211> 349<212> DNA<213> Thermus thermophilus<400> 123gggggtgaaa cggtctcgac gggggtcgcc gagggcgtgg ctgcgcgccg aggtgcgggt 60ggcctcgtaa aaacccgcaa cggcataact gccaacacca actacgctct cgcggcttaa 120tgaccgcgac ctcgcccggt agccctgccg ggggctcacc ggaagcgggg acacaaaccc 180ggctagcccg gggccacgcc ctctaacccc gggcgaagct tgaagggggc tcgctcctgg 240ccgcccgtcc gcgggccaag ccaggaggac acgcgaaacg cggactacgc gcgtagaggc 300ccgccgtaga gaccttcgga cgggggttcg actcccccca cctccacca             349<210> 124<211> 349<212> RNA<213> Thermus thermophilus<400> 124gggggugaaa cggucucgac gggggucgcc gagggcgugg cugcgcgccg aggugcgggu 60ggccucguaa aaacccgcaa cggcauaacu gccaacacca acuacgcucu cgcggcuuaa 120ugaccgcgac cucgcccggu agcccugccg ggggcucacc ggaagcgggg acacaaaccc 180ggcuagcccg gggccacgcc cucuaacccc gggcgaagcu ugaagggggc ucgcuccugg 240ccgcccgucc gcgggccaag ccaggaggac acgcgaaacg cggacuacgc gcguagaggc 300ccgccguaga gaccuucgga cggggguucg acucccccca ccuccacca             349<210> 125<211> 354<212> DNA<213> Treponema pallidum<400> 125ggggatgact aggtttcgac tagggatgtg gggtgttgcg ctgcaggtgg agtgtcgatc 60tcctgattcg gcgcctttat aactgccaat tctgacagtt tcgactacgc gctcgccgcg 120taatcgcggg cctgtgtttg cgctgctctg agcgaacata tcggcccgac gccaaacgga 180gcttgctctt acgttgtgca cggcggacgt agggggactt ttgtctgtgc taagactctg 240gcgcgtgcgg tgcaggccta gcagagtccg acaaacgcag tacgcaccgc taaacctgta 300ggcgcgcagc actcgctctt taggacgggg gttcgattcc ccccatctcc acca       354<210> 126<211> 354<212> RNA<213> Treponema pallidum<400> 126ggggaugacu agguuucgac uagggaugug ggguguugcg cugcaggugg agugucgauc 60uccugauucg gcgccuuuau aacugccaau ucugacaguu ucgacuacgc gcucgccgcg 120uaaucgcggg ccuguguuug cgcugcucug agcgaacaua ucggcccgac gccaaacgga 180gcuugcucuu acguugugca cggcggacgu agggggacuu uugucugugc uaagacucug 240gcgcgugcgg ugcaggccua gcagaguccg acaaacgcag uacgcaccgc uaaaccugua 300ggcgcgcagc acucgcucuu uaggacgggg guucgauucc ccccaucucc acca       354<210> 127<211> 367<212> DNA<213> Vibrio cholerae<400> 127ggggctgatt caggattcga cgggaatttt gcagtctgag gtgcatgccg aggtgcggta 60ggcctcgtta acaaaccgca aaaaaatagt cgcaaacgac gaaaactacg cactagcagc 120ttaataccct gctcagagcc cttcctccct agcttccgct tgtaagacgg ggaaatcagg 180aaggtcaaac caaatcaagc tggcgtggat tcccccacct gagggatgaa gcgcgagatc 240taattcaggt tagccattcg ttagcgtgtc ggttcgcagg cggtggtgaa attaaagatc 300gactaagcat gtagtaccaa agatgaatgg ttttcggacg ggggttcaac tccccccagc 360tccacca                                                           367<210> 128<211> 367<212> RNA<213> Vibrio cholerae<400> 128ggggcugauu caggauucga cgggaauuuu gcagucugag gugcaugccg aggugcggua 60ggccucguua acaaaccgca aaaaaauagu cgcaaacgac gaaaacuacg cacuagcagc 120uuaauacccu gcucagagcc cuuccucccu agcuuccgcu uguaagacgg ggaaaucagg 180aaggucaaac caaaucaagc uggcguggau ucccccaccu gagggaugaa gcgcgagauc 240uaauucaggu uagccauucg uuagcguguc gguucgcagg cgguggugaa auuaaagauc 300gacuaagcau guaguaccaa agaugaaugg uuuucggacg gggguucaac uccccccagc 360uccacca                                                           367<210> 129<211> 364<212> DNA<213> Yersinia pestis<400> 129ggggctgatt ctggattcga cgggattcgc gaaacccaag gtgcatgccg aggtgcggtg 60gcctcgtaaa aaaccgcaaa aaaaatagtt gcaaacgacg aaaactacgc actagcagct 120taataacctg cttagagccc tctctgccta gcctccgctc ttaggacggg gatcaagaga 180ggtcaaacct aaaagagctc gtgtggaaac cttgcctggg gtggaagcat taaaactaat 240caggatagtt tgtcagtagc gtgtccatcc gcagctggcc ggcgaatgta atgattggac 300taagcatgta gtgccgacgg tgtagtaatt tcggacgggg gttcaaatcc ccccagctcc 360acca                                                              364<210> 130<211> 364<212> RNA<213> Yersinia pestis<400> 130ggggcugauu cuggauucga cgggauucgc gaaacccaag gugcaugccg aggugcggug 60gccucguaaa aaaccgcaaa aaaaauaguu gcaaacgacg aaaacuacgc acuagcagcu 120uaauaaccug cuuagagccc ucucugccua gccuccgcuc uuaggacggg gaucaagaga 180ggucaaaccu aaaagagcuc guguggaaac cuugccuggg guggaagcau uaaaacuaau 240caggauaguu ugucaguagc guguccaucc gcagcuggcc ggcgaaugua augauuggac 300uaagcaugua gugccgacgg uguaguaauu ucggacgggg guucaaaucc ccccagcucc 360acca                                                              364<210> 131<211> 309<212> DNA<213> Campylobacter fetus<400> 131aggagtaagt ctgcttagat ggcatgtcgc tttgggcaaa gcgtaaaaag cccaaataaa 60attaaacgca aacaacgtta aattcgctcc tgcttacgct aaagctgcgt aagttcagtt 120gagcctgaaa tttaagtcat actatctagc ttaattttcg gtcatctttg atagtgtagc 180cttgcgtttg acaagcgttg aggtgaaata aagtcttagc cttgcttttg agttttggaa 240gatgagcgaa gtagggtgaa gtagtcatct ttgctaagca tgtagaggtc tttgtgggat 300tatttttgg                                                         309<210> 132<211> 309<212> RNA<213> Campylobacter fetus<400> 132aggaguaagu cugcuuagau ggcaugucgc uuugggcaaa gcguaaaaag cccaaauaaa 60auuaaacgca aacaacguua aauucgcucc ugcuuacgcu aaagcugcgu aaguucaguu 120gagccugaaa uuuaagucau acuaucuagc uuaauuuucg gucaucuuug auaguguagc 180cuugcguuug acaagcguug aggugaaaua aagucuuagc cuugcuuuug aguuuuggaa 240gaugagcgaa guagggugaa guagucaucu uugcuaagca uguagagguc uuugugggau 300uauuuuugg                                                         309<210> 133<211> 309<212> DNA<213> Campylobacter coli (BM2509)<400> 133aggagtaagt ctgcttagat ggcatgtcgc tttggacaaa gcgtaaaaag tccaaattaa 60aattaaacgc aaataacgtt aaatttgctc ctgcttacgc taaagctgcg taagttcagt 120tgagcccgaa actcaagtga tgctatctag cttgaatttt ggtcatcttt gatagtgtag 180attgaaaatt gacaactttt aatcgaagtt aaagtcttag tctagcttga aattttggaa 240ggtgagttta gccagatgaa gttttcacct ttgctaaaca tgtagaagtc tttgtggggt 300tatttttgg                                                         309<210> 134<211> 309<212> RNA<213> Campylobacter coli (BM2509)<400> 134aggaguaagu cugcuuagau ggcaugucgc uuuggacaaa gcguaaaaag uccaaauuaa 60aauuaaacgc aaauaacguu aaauuugcuc cugcuuacgc uaaagcugcg uaaguucagu 120ugagcccgaa acucaaguga ugcuaucuag cuugaauuuu ggucaucuuu gauaguguag 180auugaaaauu gacaacuuuu aaucgaaguu aaagucuuag ucuagcuuga aauuuuggaa 240ggugaguuua gccagaugaa guuuucaccu uugcuaaaca uguagaaguc uuuguggggu 300uauuuuugg                                                         309<210> 135<211> 311<212> DNA<213> 未知<220><223> 未知生物体的描述：Campylobacter chicken分离物<400> 135acaggagtaa gtctgcttag atggcatgtc gctttgggca aagcgtaaaa agcccaaata 60aaattaaacg caaacaacgt taaattcgct cctgcttacg ctaaagctgc gtaagttcag 120ttgagcctga aatttaagtc atactatcta gcttaatttt cggtcatttt tgatagtgta 180gccttgcgtt tgacaagcgt tgaggtgaaa taaggtctta gccttgcttt tgagttttgg 240aagatgagcg aagtagggtg aagtagtcat ctttgctaag catgtagagg tctttgtggg 300attatttttg g                                                      311<210> 136<211> 311<212> RNA<213> 未知<220><223> 未知生物体的描述：Campylobacter chicken分离物<400> 136acaggaguaa gucugcuuag auggcauguc gcuuugggca aagcguaaaa agcccaaaua 60aaauuaaacg caaacaacgu uaaauucgcu ccugcuuacg cuaaagcugc guaaguucag 120uugagccuga aauuuaaguc auacuaucua gcuuaauuuu cggucauuuu ugauagugua 180gccuugcguu ugacaagcgu ugaggugaaa uaaggucuua gccuugcuuu ugaguuuugg 240aagaugagcg aaguagggug aaguagucau cuuugcuaag cauguagagg ucuuuguggg 300auuauuuuug g                                                      311<210> 137<211> 313<212> DNA<213> Clostridium perfringens<400> 137acgggggtag gatgggtttg ataagcgagt cgagggaagc atggtgcctc gataataaag 60tatgcattaa agataaacgc acgagataat tttgcattag cagcttaagt tagcgctgct 120catccttcct caattgccca cggttgagag taagggtgtc atttaaaagt ggggaaccga 180gcctagcaaa gctttgagct aggaacggaa tttatgaagc ttaccaaaga ggaagtttgt 240ctgtggacgt tctctgaggg aattttaaaa cacaagacta cactcgtaga aagtcttact 300ggtctgcttt cgg                                                    313<210> 138<211> 313<212> RNA<213> Clostridium perfringens<400> 138acggggguag gauggguuug auaagcgagu cgagggaagc auggugccuc gauaauaaag 60uaugcauuaa agauaaacgc acgagauaau uuugcauuag cagcuuaagu uagcgcugcu 120cauccuuccu caauugccca cgguugagag uaaggguguc auuuaaaagu ggggaaccga 180gccuagcaaa gcuuugagcu aggaacggaa uuuaugaagc uuaccaaaga ggaaguuugu 240cuguggacgu ucucugaggg aauuuuaaaa cacaagacua cacucguaga aagucuuacu 300ggucugcuuu cgg                                                    313<210> 139<211> 331<212> DNA<213> Haemophilus ducreyi (NCTC 10945)<400> 139acgggattag cgaagtccaa ggtgcacgtc gaggtgcggt aggcctcgta acaaaccgca 60aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc ttaataacct gcatttagcc 120ttcgcgccct agctttcgct cgtaagacgg ggagcacgcg gagtcaaacc aaaacgagat 180cgtgtggacg cttccgcttg tagatgaaac actaaattga atcaagctag tttatttctt 240gcgtgtctgt ccgctggaga taagcgaaat taaagaccag actaaacgtg tagtactgaa 300gatagagtaa tttcggaccc gggttcgact c                                331<210> 140<211> 331<212> RNA<213> Haemophilus ducreyi (NCTC 10945)<400> 140acgggauuag cgaaguccaa ggugcacguc gaggugcggu aggccucgua acaaaccgca 60aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc uuaauaaccu gcauuuagcc 120uucgcgcccu agcuuucgcu cguaagacgg ggagcacgcg gagucaaacc aaaacgagau 180cguguggacg cuuccgcuug uagaugaaac acuaaauuga aucaagcuag uuuauuucuu 240gcgugucugu ccgcuggaga uaagcgaaau uaaagaccag acuaaacgug uaguacugaa 300gauagaguaa uuucggaccc ggguucgacu c                                331<210> 141<211> 232<212> DNA<213> Listeria innocua(食物分离物#1)<400> 141ggcaaagaaa aacaaaacct agctttcgct gcctaataac cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cagactagct gaatggaagc ctgttaccgg 180gctgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattc aa         232<210> 142<211> 232<212> RNA<213>Listeria innocua(食物分离物#1)<400> 142ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaac caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuaccgg 180gcugauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuc aa         232<210> 143<211> 232<212> DNA<213> Listeria innocua(食物分离物#2)<400> 143ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cagactagct gaatggaagc ctgttaccgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa         232<210> 144<211> 232<212> RNA<213>Listeria innocua(食物分离物#2)<400> 144ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuaccgg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa         232<210> 145<211> 232<212> DNA<213> Listeria innocua(食物分离物#3)<400> 145ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagaata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cggactagct gaatggaagc ctgttaccgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa         232<210> 146<211> 232<212> RNA<213> Listeria innocua(食物分离物#3)<400> 146ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagaaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cggacuagcu gaauggaagc cuguuaccgg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa         232<210> 147<211> 232<212> DNA<213> Listeria innocua (ATCC 12210)<400> 147ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctggg gttaaataga agagcttaat cagactagct gaatggaagc ctgttactgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa         232<210> 148<211> 232<212> RNA<213> Listeria innocua (ATCC 12210)<400> 148ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucuggg guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuacugg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa         232<210> 149<211> 322<212> DNA<213> Listeria ivanovii (NCTC 11846)<400> 149acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta ttaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg                                          322<210> 150<211> 321<212> RNA<213> Listeria ivanovii (NCTC 11846)<400> 150acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua uuaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacucg cucguagaua 300uuuaagugcc gauauuucug g                                           321<210> 151<211> 322<212> DNA<213> Listeria seeligeri (NCTC 11856)<400> 151acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggaaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatactaata cggtgactac gctcgtagat 300atttaagtgc ccatatttct gg                                          322<210> 152<211> 322<212> RNA<213> Listeria seeligeri (NCTC 11856)<400> 152acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacggaaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aauacuaaua cggugacuac gcucguagau 300auuuaagugc ccauauuucu gg                                          322<210> 153<211> 314<212> DNA<213> Salmonella enteritidis<400> 153acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca 60aaaaaatagt cgcaaacgac gaaacctacg ctttagcagc ttaataacct gcttagagcc 120ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat 180cgcgtggatg ccctgcctgg ggttgaagcg ttaaaacgaa tcaggctagt ctggtagtgg 240cgtgtccgtc cgcaggtgcc aggcgaatgt aaagactgac taagcatgta gtaccgagga 300tgtaggaatt tcgg                                                   314<210> 154<211> 314<212> RNA<213> Salmonella enteritidis<400> 154acgggauuug cgaaacccaa ggugcaugcc gaggggcggu uggccucgua aaaagccgca 60aaaaaauagu cgcaaacgac gaaaccuacg cuuuagcagc uuaauaaccu gcuuagagcc 120cucucucccu agccuccgcu cuuaggacgg ggaucaagag aggucaaacc caaaagagau 180cgcguggaug cccugccugg gguugaagcg uuaaaacgaa ucaggcuagu cugguagugg 240cguguccguc cgcaggugcc aggcgaaugu aaagacugac uaagcaugua guaccgagga 300uguaggaauu ucgg                                                   314<210> 155<211> 313<212> DNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 155acaggggtcc cccgagctta ttaagcgtgt cggagggttg gctccgtcat caacacattt 60cggttaaata taactgacaa atcaaacaat aatttcgcag tagctgcgta atagccactg 120catcgcctaa cagcatctcc tacgtgctgt taacgcgatt caaccctagt aggatatgct 180aaacactgcc gcttgaagtc tgtttagatg aaatataatc aagctagtat catgttggtt 240gtttattgct tagcatgatg cgaaaattat caataaacta cacacgtaga aagatttgta 300tcaggacctc tgg                                                    313<210> 156<211> 313<212> RNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 156acaggggucc cccgagcuua uuaagcgugu cggaggguug gcuccgucau caacacauuu 60cgguuaaaua uaacugacaa aucaaacaau aauuucgcag uagcugcgua auagccacug 120caucgccuaa cagcaucucc uacgugcugu uaacgcgauu caacccuagu aggauaugcu 180aaacacugcc gcuugaaguc uguuuagaug aaauauaauc aagcuaguau cauguugguu 240guuuauugcu uagcaugaug cgaaaauuau caauaaacua cacacguaga aagauuugua 300ucaggaccuc ugg                                                    313<210> 157<211> 302<212> DNA<213> Streptococcus agalactiae (NCTC 8181)<400> 157acaggcatta tgaggtatat tttgcgactc atcggcagat gtaaaatgcc agttaaatat 60aactgcaaaa aatacaaatt cttacgcatt agctgcctaa aaaacagcct gcgtgatctt 120cacaagattg tttgcgtttt gctagaaggt cttatttatc agcaaactac gtttggctac 180tgtctagtta gttaaaaaga gatttataga ctcgctatgt gagggcttga gttatgtgtc 240atcacctagt taaatcaata cataacctat agttgtagac aaatatatta gcagatgttt 300gg                                                                302<210> 158<211> 302<212> RNA<213> Streptococcus agalactiae (NCTC 8181)<400> 158acaggcauua ugagguauau uuugcgacuc aucggcagau guaaaaugcc aguuaaauau 60aacugcaaaa aauacaaauu cuuacgcauu agcugccuaa aaaacagccu gcgugaucuu 120cacaagauug uuugcguuuu gcuagaaggu cuuauuuauc agcaaacuac guuuggcuac 180ugucuaguua guuaaaaaga gauuuauaga cucgcuaugu gagggcuuga guuauguguc 240aucaccuagu uaaaucaaua cauaaccuau aguuguagac aaauauauua gcagauguuu 300gg                                                                302<210> 159<211> 168<212> DNA<213> Bordetella bronchiseptica<400> 159ggggccgatc cggattcgac gtgggtcatg aaacagctca aggcatgccg agcaccagta 60agctcgttaa tccactggaa cactacaaac gccaacgacg agcgtttcgc tctcgccgct 120taagcggtga gccgctgcac tgatctgtcc ttgggtcacg cgggggaa              168<210> 160<211> 168<212> RNA<213> Bordetella bronchiseptica<400> 160ggggccgauc cggauucgac gugggucaug aaacagcuca aggcaugccg agcaccagua 60agcucguuaa uccacuggaa cacuacaaac gccaacgacg agcguuucgc ucucgccgcu 120uaagcgguga gccgcugcac ugaucugucc uugggucacg cgggggaa              168<210> 161<211> 426<212> DNA<213> Chlamydia pneumoniae (CWL029)<400> 161gggggtgtat aggtttcgac ttgaaaatga agtgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccaaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120tagcttgttt gactcagtag aggaaagact agctgcttaa ttagcaaaag ttgttagcta 180gataatctct aggtaacccg gtatctgcga gctccaccag aggcttgcaa aataccgtca 240tttatctggt tggaacttac tttctctaat tctcaaggaa gttcgttcga gatttttgag 300agtcattggc tgctatagag gcttctagct aagggagtcc aatgtaaaca attctagaag 360ataagcatgt agaggttagc agggagtttg tcaaggacga gagttcgagt ctctccacct 420ccacca                                                            426<210> 162<211> 426<212> RNA<213> Chlamydia pneumoniae (CWL029)<400> 162ggggguguau agguuucgac uugaaaauga aguguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccaaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120uagcuuguuu gacucaguag aggaaagacu agcugcuuaa uuagcaaaag uuguuagcua 180gauaaucucu agguaacccg guaucugcga gcuccaccag aggcuugcaa aauaccguca 240uuuaucuggu uggaacuuac uuucucuaau ucucaaggaa guucguucga gauuuuugag 300agucauuggc ugcuauagag gcuucuagcu aagggagucc aauguaaaca auucuagaag 360auaagcaugu agagguuagc agggaguuug ucaaggacga gaguucgagu cucuccaccu 420ccacca                                                            426<210> 163<211> 421<212> DNA<213> Francisella tularensis<400> 163gggggcgaat atggtttcga catgaatgtc aaaatctaag gtgcatgccg aggaagtacc 60gtaacctcgt taataacagt acaaatgcca ataataactg gcaacaaaaa agcaaaccgc 120gtagcggcta acgacagcaa ctttgctgct gttgctaaag ctgcctagtc tagcttaata 180atctagatgc gcacggatat gatagtcttt cttatgacac tatctataca tccgttcata 240ttccgcataa gacggtcttt gctttttgtc tgggagttaa ggctgtattt aacagactcg 300ctaactatta ccctggctaa ttggggaata gtcaagctaa actcaaatag attagcctaa 360gcatgtagat ccaaagatct agagtttgtg gacgcgggtt caaatcccgc cgcctccacc 420a                                                                 421<210> 164<211> 421<212> RNA<213> Francisella tularensis<400> 164gggggcgaau augguuucga caugaauguc aaaaucuaag gugcaugccg aggaaguacc 60guaaccucgu uaauaacagu acaaaugcca auaauaacug gcaacaaaaa agcaaaccgc 120guagcggcua acgacagcaa cuuugcugcu guugcuaaag cugccuaguc uagcuuaaua 180aucuagaugc gcacggauau gauagucuuu cuuaugacac uaucuauaca uccguucaua 240uuccgcauaa gacggucuuu gcuuuuuguc ugggaguuaa ggcuguauuu aacagacucg 300cuaacuauua cccuggcuaa uuggggaaua gucaagcuaa acucaaauag auuagccuaa 360gcauguagau ccaaagaucu agaguuugug gacgcggguu caaaucccgc cgccuccacc 420a                                                                 421<210> 165<211> 330<212> DNA<213> Guillardia theta (质体)<400> 165ggggctgatt tggattcgac atataaattt gcgtgtttca ttatgaagca agtcaagttt 60aatgatcttg taaaaaacat taaagtacaa ataaatgcaa gcaatatagt ttcatttagt 120tcaaaacgtt tagtctcttt tgcataagca aaatgtgtta ataactttct tagtagaaat 180tggagaagtt tactaagatt tatatttact ccataattat tttaaagatg gtaaaaaggt 240gattcatcat ttgtatgttt ctaaactttg tgaaagaata gtgggctcca tttataatga 300acgtgggttc aaatcccacc agctccacca                                  330<210> 166<211> 330<212> RNA<213> Guillardia theta(质体)<400> 166ggggcugauu uggauucgac auauaaauuu gcguguuuca uuaugaagca agucaaguuu 60aaugaucuug uaaaaaacau uaaaguacaa auaaaugcaa gcaauauagu uucauuuagu 120ucaaaacguu uagucucuuu ugcauaagca aaauguguua auaacuuucu uaguagaaau 180uggagaaguu uacuaagauu uauauuuacu ccauaauuau uuuaaagaug guaaaaaggu 240gauucaucau uuguauguuu cuaaacuuug ugaaagaaua gugggcucca uuuauaauga 300acguggguuc aaaucccacc agcuccacca                                  330<210> 167<211> 348<212> DNA<213> Thalassiosira Weissflogii(质体)<400> 167ggggctgatt tggtttcgac atttaaaact tctttctatg tgtcaggtca aagtttgtat 60tctttgtaaa aaaatactaa aatactaata aatgctaata atataatacc gtttattttt 120aaagcagtaa aaacaaaaaa agaagcaatg gctttaaatt ttgctgtata gttcattaac 180ttaggttatt aaatattttt tcattataac tggacttttc tctagtttat agtttagaat 240aaatttaaat tttgcaaaac tcgttcgaaa attttcgggc taaacctgta aacgcaaata 300ctaagaaatt ttagatggac atgggttcaa ttcccatcag ttccacca              348<210> 168<211> 348<212> RNA<213> Thalassiosira Weissflogii(质体)<400> 168ggggcugauu ugguuucgac auuuaaaacu ucuuucuaug ugucagguca aaguuuguau 60ucuuuguaaa aaaauacuaa aauacuaaua aaugcuaaua auauaauacc guuuauuuuu 120aaagcaguaa aaacaaaaaa agaagcaaug gcuuuaaauu uugcuguaua guucauuaac 180uuagguuauu aaauauuuuu ucauuauaac uggacuuuuc ucuaguuuau aguuuagaau 240aaauuuaaau uuugcaaaac ucguucgaaa auuuucgggc uaaaccugua aacgcaaaua 300cuaagaaauu uuagauggac auggguucaa uucccaucag uuccacca              348<210> 169<211> 19<212> DNA<213> 人工序列<220><223> 人工序列的描述：V.cholerae tmRNA特异性探针<400> 169aacgaatggc taacctgaa                                              19<210> 170<211> 17<212> DNA<213> 人工序列<220><223> 人工序列的描述：通用ssrA/tmRNA 5’体外扩增引物<400> 170gggmytacgg wttcgac                                                17<210> 171<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：通用ssrA/tmRNA 3’体外扩增引物<400> 171gggartcgaa ccrsgtcc                                               18<210> 172<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：5’Listeria属特异性PCR扩增引物<400> 172aaagccaata ataactgg                                               18<210> 173<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：3’Listeria属特异性扩增引物<400> 173ccagaaatat cggcactt                                              18<210> 174<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：Listeria属特异性杂交探针<400> 174gtgagaccct taccgtag                                              18<210> 175<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：Listeria monocytogenes种特异性杂交探针<400> 175tctatttaac cccagacg                                              18<210> 176<211> 348<212> DNA<213> Helicobacter pylori<400> 176tggggatgtt acggtttcga caggggtagt tcgagcttag gtggcgagtc gaggggatcg 60gcctcgttaa aacgtcaaag cctataactg gcaaacaaca aaacaacttc gctttagcag 120cttaataagc tcttagcggt tcctccctcc atcgcccatg tggtagggta agggactcaa 180attaagtggg ctacgctgga ttccaccgtc tgaggatgaa agaagagaac aaccagacta 240gctacccgga cgcccgtcga taggcagatg gagtagcgaa tcgcgaatat atcgactaca 300ctcgtagaag cttaagtgcc gatattcttg gacgtgggtt cgactccc              348<210> 177<211> 348<212> RNA<213> Helicobacter pylori<400> 177uggggauguu acgguuucga cagggguagu ucgagcuuag guggcgaguc gaggggaucg 60gccucguuaa aacgucaaag ccuauaacug gcaaacaaca aaacaacuuc gcuuuagcag 120cuuaauaagc ucuuagcggu uccucccucc aucgcccaug ugguagggua agggacucaa 180auuaaguggg cuacgcugga uuccaccguc ugaggaugaa agaagagaac aaccagacua 240gcuacccgga cgcccgucga uaggcagaug gaguagcgaa ucgcgaauau aucgacuaca 300cucguagaag cuuaagugcc gauauucuug gacguggguu cgacuccc              348<210> 178<211> 344<212> DNA<213> Helicobacter pylori<400> 178tggggacgtt acggtttcga cagggatagt tcgagcttag gttgcgagtc gaggggatcg 60gcctcgttaa aacgtcaaag cctataattg gcaaacaaaa caatctttct ttagctgctt 120aattgcacta aaggttcctc cctccatcgt ccatgtggta gggtaaggga ctcaaactaa 180gtggactacg ccggagttcg ccgtctgagg acaaaggaag agaacaacca gactagcaac 240ttggaagcct gtcgataggc cgaagagttc gcgaaatgct aatatatcga ctacactcgt 300agaagcttaa gtgccgatat ttttggacgt gggttcgatt ccct                  344<210> 179<211> 344<212> RNA<213> Helicobacter pylori<400> 179uggggacguu acgguuucga cagggauagu ucgagcuuag guugcgaguc gaggggaucg 60gccucguuaa aacgucaaag ccuauaauug gcaaacaaaa caaucuuucu uuagcugcuu 120aauugcacua aagguuccuc ccuccaucgu ccauguggua ggguaaggga cucaaacuaa 180guggacuacg ccggaguucg ccgucugagg acaaaggaag agaacaacca gacuagcaac 240uuggaagccu gucgauaggc cgaagaguuc gcgaaaugcu aauauaucga cuacacucgu 300agaagcuuaa gugccgauau uuuuggacgu ggguucgauu cccu                  344<210> 180<211> 322<212> DNA<213> Listeria seeligeri(NCTC 11856)<400> 180acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggaaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatactaata cggtgactac gctcgtagat 300atttaagtgc ccatatttct gg                                          322<210> 181<211> 322<212> RNA<213> Listeria seeligeri (NCTC 11856)<400> 181acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacggaaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aauacuaaua cggugacuac gcucguagau 300auuuaagugc ccauauuucu gg                                          322<210> 182<211> 322<212> DNA<213> Listeria ivanovii (NCTC 11846)<400> 182acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta ttaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg                                          322<210> 183<211> 321<212> RNA<213> Listeria ivanovii (NCTC 11846)<400> 183acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua uuaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacucg cucguagaua 300uuuaagugcc gauauuucug g                                           321<210> 184<211> 319<212> DNA<213> Mycobacterium africanum<400> 184acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcgacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaacgccctc ggcccggacc ctggcatcag ctagagggat 180ccaccgatga gtccggtcgc gggactcctc gggacaacca cagcgactgg gatcgtcatc 240tcggctagtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta                                              319<210> 185<211> 319<212> RNA<213> Mycobacterium africanum<400> 185acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcgacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaacgcccuc ggcccggacc cuggcaucag cuagagggau 180ccaccgauga guccggucgc gggacuccuc gggacaacca cagcgacugg gaucgucauc 240ucggcuaguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua                                              319<210> 186<211> 319<212> DNA<213> Mycobacterium gordonae<400> 186acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca tataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcggaccgg gaacgccctc gccccggacc ccggcatcag ctagagggat 180caaccgatga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtc cgtgtgacca ggagatccga gcagagacat agcggactgc gcacggagaa 300gccttgaggg aatgccgta                                              319<210> 187<211> 319<212> RNA<213> Mycobacterium gordonae<400> 187acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca uauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucggaccgg gaacgcccuc gccccggacc ccggcaucag cuagagggau 180caaccgauga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguc cgugugacca ggagauccga gcagagacau agcggacugc gcacggagaa 300gccuugaggg aaugccgua                                              319<210> 188<211> 319<212> DNA<213> Mycobacterium kansasii<400> 188acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaccgccctc gacccggact ctggcatcag ctagagggat 180caaccgatga gttcggtcgc gggactcgtc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta                                              319<210> 189<211> 319<212> RNA<213> Mycobacterium kansasii<400> 189acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaccgcccuc gacccggacu cuggcaucag cuagagggau 180caaccgauga guucggucgc gggacucguc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua                                              319<210> 190<211> 320<212> DNA<213> Mycobacterium chelonae<400> 190acagcgagtc tcgacttaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60attgcaacca attaagcgcc gattctcatc agcgcgacta cgcactcgct gcctaagcga 120ctgcgtgtct gtcagaccgg gagcgccctc agcccggacc ctggcatcag ctagagggac 180aaactacggg ttcggtcgcg ggacccgtag ggacatcaaa cagcgactgg gatcgtcatc 240tcggcttgtt cgcgggaccg agagatccaa gtagaggcat agcgaactgc gcacggagaa 300gccttaatga acggccgttg                                             320<210> 191<211> 320<212> RNA<213> Mycobacterium chelonae<400> 191acagcgaguc ucgacuuaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60auugcaacca auuaagcgcc gauucucauc agcgcgacua cgcacucgcu gccuaagcga 120cugcgugucu gucagaccgg gagcgcccuc agcccggacc cuggcaucag cuagagggac 180aaacuacggg uucggucgcg ggacccguag ggacaucaaa cagcgacugg gaucgucauc 240ucggcuuguu cgcgggaccg agagauccaa guagaggcau agcgaacugc gcacggagaa 300gccuuaauga acggccguug                                             320<210> 192<211> 320<212> DNA<213> Mycobacterium szulgai<400> 192acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gagaacactc agcgcgactt cgctctcgct gcctaagcga 120cagcaagtcc gtcagaccgg gaaagccctc gacccggacc ctggcgtcat ctagagggat 180ccaccggtga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gtagagacat agcgaactgc gcacggagaa 300gccttgaggg aatgccgtag                                             320<210> 193<211> 320<212> RNA<213> Mycobacterium szulgai<400> 193acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gagaacacuc agcgcgacuu cgcucucgcu gccuaagcga 120cagcaagucc gucagaccgg gaaagcccuc gacccggacc cuggcgucau cuagagggau 180ccaccgguga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga guagagacau agcgaacugc gcacggagaa 300gccuugaggg aaugccguag                                             320<210> 194<211> 320<212> DNA<213> Mycobacterium malmoense<400> 194acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca tataagcgcc gtttcaacac agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaacgccctc gacccggagc ctggcgtcag ctggagggat 180ccaccggtga gtccggtcgc gggactcatc gggacataca cagcgactgg gatcgtcatc 240ctggctggtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgtag                                             320<210> 195<211> 320<212> RNA<213> Mycobacterium malmoense<400> 195acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca uauaagcgcc guuucaacac agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaacgcccuc gacccggagc cuggcgucag cuggagggau 180ccaccgguga guccggucgc gggacucauc gggacauaca cagcgacugg gaucgucauc 240cuggcugguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccguag                                             320<210> 196<211> 321<212> DNA<213> Mycobacterium flavescens<400> 196acttcgagcg tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattccaatc agcgcgacta cgcactcgct gcctaagcga 120ctgcgtgtct gtcagcccgg gagagccctc gacccggtgt ctggcatcag ctagagggat 180aaaccggtgg gtccggtcgc gggactcatc gggacatcaa acagcgactg ggatcgtcat 240cctgacttgt tcgcgtgatc aggagatccg agtagagaca tagcgaactg cgcacggaga 300agccttgagg gaacgccgta g                                           321<210> 197<211> 321<212> RNA<213> Mycobacterium flavescens<400> 197acuucgagcg ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauuccaauc agcgcgacua cgcacucgcu gccuaagcga 120cugcgugucu gucagcccgg gagagcccuc gacccggugu cuggcaucag cuagagggau 180aaaccggugg guccggucgc gggacucauc gggacaucaa acagcgacug ggaucgucau 240ccugacuugu ucgcgugauc aggagauccg aguagagaca uagcgaacug cgcacggaga 300agccuugagg gaacgccgua g                                           321<210> 198<211> 320<212> DNA<213> Mycobacterium marinum<400> 198acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gatgcaacta gataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcggaccgg gaacgccctc gccccggacc ccggcatcag ctagagggat 180caaccgatga gttcggtcgc ggggctcatc gggacatcaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gcagagacct agcggactgc gcacggagaa 300gccttgaggg aatgccgtag                                             320<210> 199<211> 320<212> RNA<213> Mycobacterium marinum<400> 199acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60gaugcaacua gauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucggaccgg gaacgcccuc gccccggacc ccggcaucag cuagagggau 180caaccgauga guucggucgc ggggcucauc gggacaucaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga gcagagaccu agcggacugc gcacggagaa 300gccuugaggg aaugccguag                                             320<210> 200<211> 319<212> DNA<213> Mycobacterium microti<400> 200acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcgacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaacgccctc ggcccggacc ctggcatcag ctagagggat 180ccaccgatga gtccggtcgc gggactcctc gggacagcca cagcgactgg gatcgtcatc 240tcggctagtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta                                              319<210> 201<211> 319<212> RNA<213> Mycobacterium microti<400> 201acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcgacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaacgcccuc ggcccggacc cuggcaucag cuagagggau 180ccaccgauga guccggucgc gggacuccuc gggacagcca cagcgacugg gaucgucauc 240ucggcuaguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua                                              319<210> 202<211> 321<212> DNA<213> Mycobacterium smegmatis<400> 202acttcgagca tcgaatccag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattccaatc agcgcgacta cgccctcgct gcctaagcga 120cggctggtct gtcagaccgg gagtgccctc ggcccggatc ctggcatcag ctagagggac 180ccacccacgg gttcggtcgc gggacctgtg gggacatcaa acagcgactg ggatcgtcat 240ctcggcttgt tcgtgtgacc gggagatccg agtagagaca tagcgaactg cgcacggaga 300agcctcgagg acatgccgta g                                           321<210> 203<211> 321<212> RNA<213> Mycobacterium smegmatis<400> 203acuucgagca ucgaauccag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauuccaauc agcgcgacua cgcccucgcu gccuaagcga 120cggcuggucu gucagaccgg gagugcccuc ggcccggauc cuggcaucag cuagagggac 180ccacccacgg guucggucgc gggaccugug gggacaucaa acagcgacug ggaucgucau 240cucggcuugu ucgugugacc gggagauccg aguagagaca uagcgaacug cgcacggaga 300agccucgagg acaugccgua g                                           321<210> 204<211> 320<212> DNA<213> Mycobacterium xenopi<400> 204acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacta aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcaggccgg gagttccctc gacccggatc ctggcgtcag ctagagggat 180ccaccgatgg gttcggtcgc gggacccatc gggacaccac acagcgactg ggatcgccgt 240cccggctagt tcgcgagacc gggagatccg agtaagggca aagcgaactg cgcacggaga 300agccttgagg gtatgccgta                                             320<210> 205<211> 320<212> RNA<213> Mycobacterium xenopi<400> 205acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacua aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucaggccgg gaguucccuc gacccggauc cuggcgucag cuagagggau 180ccaccgaugg guucggucgc gggacccauc gggacaccac acagcgacug ggaucgccgu 240cccggcuagu ucgcgagacc gggagauccg aguaagggca aagcgaacug cgcacggaga 300agccuugagg guaugccgua                                             320<210> 206<211> 320<212> DNA<213> Mycobacterium intracellulare<400> 206acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aaccgaccac cgtaagcgtc 60gttgcaaaca gataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaacgccctc gacccggagc ctggcgtcag ctagagggat 180ccaccgatga gtccggtcgc gggacttatc gggacaccaa cagcgactgg gatcgtcatc 240tcggcttgtt cgcgtgaccg ggagatccga gtagaggcat agcgaactgc gcacggagaa 300gtcttgaggg aatgccgtag                                             320<210> 207<211> 320<212> RNA<213> Mycobacterium intracellulare<400> 207acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aaccgaccac cguaagcguc 60guugcaaaca gauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaacgcccuc gacccggagc cuggcgucag cuagagggau 180ccaccgauga guccggucgc gggacuuauc gggacaccaa cagcgacugg gaucgucauc 240ucggcuuguu cgcgugaccg ggagauccga guagaggcau agcgaacugc gcacggagaa 300gucuugaggg aaugccguag                                             320<210> 208<211> 320<212> DNA<213> Mycobacterium scrofulaceum<400> 208acatcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaaagccctc gacccggagc ctggcgtcag ctagagggat 180caaccgatga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtc cgcgtgacca ggagatccga gcagaggcat agcggactgc gcacggagaa 300gtcttgaggg aatgccgttg                                             320<210> 209<211> 320<212> RNA<213> Mycobacterium scrofulaceum<400> 209acaucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaaagcccuc gacccggagc cuggcgucag cuagagggau 180caaccgauga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguc cgcgugacca ggagauccga gcagaggcau agcggacugc gcacggagaa 300gucuugaggg aaugccguug                                             320<210> 210<211> 326<212> DNA<213> Nocardia asteroides<400> 210actgtgtgcg ccgaggtagg ggaagcgtgt cggtgcaggc tggagaccac cgttaagcgt 60cgcggcaacc aattaagcgc cgattccaat cagcgcgact acgccctcgc tgcctgatca 120gcgacggcta gctgtcggcc cgggttgtgt tcccgaaccc ggatgccggc atcatctcag 180ggaactcacc gtgttcgccg gtcgcggacg gacacgggac agcaaacagc gactgggatc 240gtcatctcgg cttgttcgcg tgaccgggag atccaagtag agacatagcg gactgcacac 300ggagaagccc tactgactcg acacag                                      326<210> 211<211> 325<212> RNA<213> Nocardia asteroides<400> 211acugugugcg ccgagguagg ggaagcgugu cggugcaggc uggagaccac cguuaagcgu 60cgcggcaacc aauuaagcgc cgauuccaau cagcgcgacu acgcccucgc ugccugauca 120gcgacggcua gcugucggcc cggguugugu ucccgaaccc ggaugccggc aucaucucag 180ggaacucacc guguucgccg gucgcggacg gacacgggac agcaaacagc gacugggauc 240gucaucucgg cuuguucgcg ugaccgggag auccaaguag agacauagcg gcugcacacg 300gagaagcccu acugacucga cacag                                       325<210> 212<211> 314<212> DNA<213> Salmonella enteritidis<400> 212acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca 60aaaaaatagt cgcaaacgac gaaacctacg ctttagcagc ttaataacct gcttagagcc 120ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat 180cgcgtggatg ccctgcctgg ggttgaagcg ttaaaacgaa tcaggctagt ctggtagtgg 240cgtgtccgtc cgcaggtgcc aggcgaatgt aaagactgac taagcatgta gtaccgagga 300tgtaggaatt tcgg                                                   314<210> 213<211> 314<212> RNA<213> Salmonella enteritidis<400> 213acgggauuug cgaaacccaa ggugcaugcc gaggggcggu uggccucgua aaaagccgca 60aaaaaauagu cgcaaacgac gaaaccuacg cuuuagcagc uuaauaaccu gcuuagagcc 120cucucucccu agccuccgcu cuuaggacgg ggaucaagag aggucaaacc caaaagagau 180cgcguggaug cccugccugg gguugaagcg uuaaaacgaa ucaggcuagu cugguagugg 240cguguccguc cgcaggugcc aggcgaaugu aaagacugac uaagcaugua guaccgagga 300uguaggaauu ucgg                                                   314<210> 214<211> 313<212> DNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 214acaggggtcc cccgagctta ttaagcgtgt cggagggttg gctccgtcat caacacattt 60cggttaaata taactgacaa atcaaacaat aatttcgcag tagctgcgta atagccactg 120catcgcctaa cagcatctcc tacgtgctgt taacgcgatt caaccctagt aggatatgct 180aaacactgcc gcttgaagtc tgtttagatg aaatataatc aagctagtat catgttggtt 240gtttattgct tagcatgatg cgaaaattat caataaacta cacacgtaga aagatttgta 300tcaggacctc tgg                                                    313<210> 215<211> 313<212> RNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 215acaggggucc cccgagcuua uuaagcgugu cggaggguug gcuccgucau caacacauuu 60cgguuaaaua uaacugacaa aucaaacaau aauuucgcag uagcugcgua auagccacug 120caucgccuaa cagcaucucc uacgugcugu uaacgcgauu caacccuagu aggauaugcu 180aaacacugcc gcuugaaguc uguuuagaug aaauauaauc aagcuaguau cauguugguu 240guuuauugcu uagcaugaug cgaaaauuau caauaaacua cacacguaga aagauuugua 300ucaggaccuc ugg                                                    313<210> 216<211> 302<212> DNA<213> Streptococcus agalactiae (NCTC 8181)<400> 216acaggcatta tgaggtatat tttgcgactc atcggcagat gtaaaatgcc agttaaatat 60aactgcaaaa aatacaaatt cttacgcatt agctgcctaa aaaacagcct gcgtgatctt 120cacaagattg tttgcgtttt gctagaaggt cttatttatc agcaaactac gtttggctac 180tgtctagtta gttaaaaaga gatttataga ctcgctatgt gagggcttga gttatgtgtc 240atcacctagt taaatcaata cataacctat agttgtagac aaatatatta gcagatgttt 300gg                                                                302<210> 217<211> 302<212> RNA<213> Streptococcus agalactiae (NCTC 8181)<400> 217acaggcauua ugagguauau uuugcgacuc aucggcagau guaaaaugcc aguuaaauau 60aacugcaaaa aauacaaauu cuuacgcauu agcugccuaa aaaacagccu gcgugaucuu 120cacaagauug uuugcguuuu gcuagaaggu cuuauuuauc agcaaacuac guuuggcuac 180ugucuaguua guuaaaaaga gauuuauaga cucgcuaugu gagggcuuga guuauguguc 240aucaccuagu uaaaucaaua cauaaccuau aguuguagac aaauauauua gcagauguuu 300gg                                                                302<210> 218<211> 21<212> DNA<213> 人工序列<220><223> 人工序列的描述：Salmonella属特异性探针<400> 218cgaatcaggc tagtctggta g                                           21<210> 219<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述：检测肺结核复合体的寡核苷酸探针<400> 219actcctcggg acarccacag cga                                         23<210> 220<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述：检测M.avium和M.Paratuberculosis序列的寡核苷酸探针<400> 220gttgcaaata gataagcgcc                                             20<210> 221<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述：检测M.Avium和M.paratuberculosis序列的寡核苷酸探针<400> 221tccgtcagcc cgggaacgcc                                             20<210> 222<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：在对Listeria菌细胞进行热杀灭处理后在tmRNA完整性的
                  测定中所用寡核苷酸探针<400> 222ttttgttttt ctttgcca                                               18<210> 223<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：在对Escherichia coli细胞进行热杀灭处理后在tmRNA完整性的
                 测定中所用的寡核苷酸探针<400> 223agttttcgtc gtttgcga                                               18<210> 224<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述：用于扩增所有分支杆菌序列的简并寡核苷酸引物<400> 224caggcaashg accaccgtaa                                             20<210> 225<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述：用于扩增所有分支杆菌序列的简并寡核苷酸引物<400> 225ggatctccyg rtcwcrcgra cwa                                         23<210> 226<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：用于扩增M.avium和M.paratuberculosis序列的寡核苷酸引物<400> 226tgccggtgca ggcaactg                                               18<210> 227<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述：用于扩增M.avium和M.Paratuberculosis序列的寡核苷酸引物<400> 227cacgcgaaca agccagga                                               18<210> 228<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述：用于检测Listeria ssrA基因序列的寡核苷酸探针<400> 228cattaaactt tagcaaggaa gtg                                         23

Claims (29)

1.ssrA基因或其片段的用途，其作为用于原核生物或真核生物的核酸探针检验中的靶点区。
2.根据权利要求1的用途，其中将ssrA基因分子中与从该DNA分子的5’末端起的高同源区相对应的片段作为通用靶点序列。
3.根据权利要求1的用途，其中将ssrA基因分子中与从该DNA分子的3’末端起的高同源区相对应的片段作为通用靶点序列。
4.根据权利要求1的用途，其中将ssrA基因分子中与低同源区相对应的片段作为核酸探针检验中的靶点区用于区分不同物种。
5.根据权利要求1的用途，其中将ssrA基因分子中与低同源区相对应的片段作为靶点区用于产生属特异性探针。
6.tmRNA即ssrA基因的RNA转录序列或其片段的用途，其作为用于原核生物或真核生物的核酸探针检验中的靶点区。
7.根据权利要求6的用途，其中将tmRNA分子中与从tmRNA分子的5’末端起的高同源区相对应的片段作为通用靶点序列。
8.根据权利要求6的用途，其中将tmRNA分子中与从tmRNA分子的3’末端起的高同源区相对应的片段作为通用靶点序列。
9.根据权利要求6的用途，其中将tmRNA分子中与低同源区相对应的片段作为核酸探针检验中的靶点区用于区分不同物种。
10.根据权利要求6的用途，其中将tmRNA分子中与低同源区相对应的片段作为靶点区用于产生属特异性探针。
11.根据前面的任一权利要求的用途，其中所说的ssrA基因片段或tmRNA片段被用作为在扩增中所用的引物的基础。
12.根据权利要求11的用途，其中扩增产物被用来作为核酸探针检验的靶点区。
13.根据权利要求6-12中的任何一项所述的用途，其中tmRNA分子的cDNA转录序列被用作为核酸杂交检验中的探针。
14.根据前面的任一权利要求所述的用途，其中所述的检验在体外完成。
15.根据权利要求1-13中的任何一项所述的用途，其中所述的检验在原位完成。
16.根据前面任一权利要求所述的用途，其中的靶点区被作为用于区分活的和死的原核生物或真核生物的检验的基础。
17.根据前面任一权利要求所述的用途，其中的靶点区被用于多探针形式以大范围地检测和/或鉴定原核生物或真核生物。
18.根据权利要求17的用途，其中的ssrA基因探针或tmRNA转录序列探针被连接到微阵列基因芯片系统上，用于大范围地高通量检测和鉴定原核生物或真核生物。
19.根据前面任一权利要求所述的用途，其中的靶点区被用作为用于检测目标样品中原核生物或真核生物的检验中的探针。
20.根据权利要求1-13中的任何一项所述的用途，其中的ssrA基因或tmRNA转录序列的片段被用于得到原核生物或真核生物的DNA图谱的检验中，从而区分同一物种的不同株系。
21.根据权利要求1-13中的任何一项所述的用途，其中的ssrA基因、tmRNA转录序列或与其互补的DNA序列或其片段被用来设计用于治疗目的来对抗感染性的原核生物或真核生物的试剂。
22.根据权利要求1-15中的任何一项所述的用途，其中的靶点区被用于监测对抗感染性媒介的药物的治疗效率。
23.根据权利要求1-15中的任何一项所述的用途，其中的靶点区被用于监测胃肠道中促进健康的微生物的存活率和水平。
24.根据权利要求1-15中的任何一项所述的用途，其中所述检验被用于原核生物或真核生物的定量分析。
25.根据权利要求1-5和11-15中的任何一项所述的用途，其中ssrA基因序列的数据库被用来鉴定原核生物或真核生物。
26.ssrA序列，其选自：SEQ ID NO：47、49、51、53、55、57、59、61、65、67、71、99、159、161、163、165、167、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214和216。
27.tmRNA序列，其选自：SEQ ID NO：48、50、52、54、56、58、60、62、66、68、72、98、160、162、164、168、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215和217。
28.寡核苷酸探针，其选自：SEQ ID NO：169、174、175、218-223和228。
29.寡核苷酸引物，其选自：SEQ ID NO：170-173和224-227。
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