CN1346984A - High-speed automatic colorimetric analyzer - Google Patents
High-speed automatic colorimetric analyzer Download PDFInfo
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- CN1346984A CN1346984A CN 01131524 CN01131524A CN1346984A CN 1346984 A CN1346984 A CN 1346984A CN 01131524 CN01131524 CN 01131524 CN 01131524 A CN01131524 A CN 01131524A CN 1346984 A CN1346984 A CN 1346984A
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Abstract
A high-speed automatic colorimetric analyzer has an automatic liquid sampling/injecting unit, a colorating/chlorimetric unit and a single-chip processor for controlling the colorating/chlorimetric operation. Said automatic liquid sampling/injecting unit uses an electric 8-way valve for connecting and switching between high accuracy plunger pump and injection tubes. Said plunger pump is controlled by single-chip processor for regulating the sampling or injection of liquid specimen, reagent, or assistant liquid. said colorating/chlorimetric unit consists of open colorating pool, liquid-delivery tube and miniature spectrophotometer. Said open colorating pool has heater and electromagnetic stirrer. Its advantages are high speed and precision, wide analyzed range, and in-line analysis.
Description
Technical field: the invention belongs to the colorimetric analysis instrument, relate in particular to the technological improvement of RAPID COLORIMETRIC ASSAY FOR instrument.
Background technology: colorimetric analysis is one of important analysis method in the analytical chemistry, but in the present colorimetric analysis, behind the transparent cuvette splendid attire sample liquid of many employings, place optical system for testing to make colorimetric again, mainly manual operations in the process, not only operate loaded down with trivial detailsly, and easily produce personal error, the requirement of more not competent on-line analysis; Existing flow injection method is carried out the report of RAPID COLORIMETRIC ASSAY FOR and the commodity of moulding both at home and abroad, what it adopted is the circulation colorimetric pool, but shortcoming is only to be suitable for the fireballing micro-example analysis of chromogenic reaction, then can not be suitable for for the slow-footed sample of chromogenic reaction, because to the slow-footed sample of chromogenic reaction, can only arrhea heating to accelerate chromogenic reaction, disturb colorimetric, in fact be difficult to do online, RAPID COLORIMETRIC ASSAY FOR but in heating process, can produce bubble; In addition, this quasi-instrument all adopts peristaltic pump to measure extraction and adds sample, reagent, possible in theory, but be difficult to guarantee from the measuring accuracy of practical function peristaltic pump.
Summary of the invention: technical matters to be solved by this invention is the high-speed automatic colorimetric analyzer that will provide that a kind of principle is simple, analysis speed is fast, precision is high, analyst coverage is wide, be easy to be implemented in line analysis.
For this reason, technical scheme of the present invention is: a kind of high-speed automatic colorimetric analyzer, by adopt automatically/impregnator, all unit of colour developing/colour comparator connect in regular turn and form, and gather Various types of data respectively from above-mentioned all unit by control system, the control analysis instrument carries out colorimetric analysis, demonstration automatically after the data processing, it is characterized in that: described adopting automatically/impregnator, be to adopt electronic multiple valve, as the coupling arrangement of high precision piston pump butt joint feed tube, liquid injection pipe and adopt the switching device shifter of liquid, fluid injection state.
Described electronic multiple valve is eight or 12 channel-types; Its valve opening is divided into three groups, wherein A group valve opening is connected sample liquid bath, reagent trough, boosting liquid (or eluent) groove respectively, high precision piston pump of B group each self-closing of valve opening, C group valve opening converges the back and connects colour developing/colour comparator inlet, and certain sampling ring to being serially connected with hollow between valve opening or filling enriching column.
Described control system is made up of single-chip microcomputer and output/input peripheral thereof, it is provided with photometric signal to adopting the control loop of liquid, reservoir quantity, can control described high precision piston pump according to the luminosity value in the colour developing/colour comparator, realize sample liquid, reagent and the collection of boosting liquid (or eluent), the adjusting of injection rate IR.
Described colour developing/colour comparator is to be composed in series by infusion pipeline by the microspectrophotometer of discrete each other uncovered colour developing pond with the band flow cell.
Described uncovered colour developing pond open topped is communicated with atmosphere, periphery is provided with the temperature control well heater, its underpart is provided with magnetic stirrer, institute's inlet of opening by threeway with adopt automatically/impregnator and a peristaltic pump be connected, its liquid outlet by three-way solenoid valve respectively with microspectrophotometer in the flow cell inlet and distill tank and be connected; The center of the microspectrophotometer of described band flow cell is a flow cell, the flow cell inlet is connected with the three-way solenoid valve of bottom, uncovered colour developing pond, outlet is connected with a peristaltic pump, and is respectively equipped with monochromatic collimated beam source and photoelectric commutator in the relative both sides of flow cell.
Described high precision piston pump is made of the stepper motor driven syringe of metering, and its measuring accuracy of adopting, annotating sample reaches the 0.87 μ l/ step.
The present invention is owing to adopted sampling with high precision, priming device, and it is adopted, the fluid injection precision improves greatly and even reach micro updating, has improved analytical accuracy and reappearance; Single-chip microcomputer is controlled high precision piston pump to adopting liquid, fluid injection enforcement adjusting with photometric signal, realized the robotization that constant is gathered to the sample liquid of the big concentration change of trace, enlarged the concentration range of liquor analysis, and made at the liquor analysis of variable concentrations accurate more, reliable; Colour developing/colour comparator is taked with the colour developing pond of temperature control, stirring and the discrete structure of microspectrophotometer of band flow cell, can improve chromogenic reaction speed greatly by heating, the bubble that can avoid again heating generation enters in the flow cell of microspectrophotometer, interference ratio coloured light road; That joins comprises with many rings, the coordination control of single-chip microcomputer to all detecting units: set heating-up temperature/time, the automatic emptying of peristaltic pump etc. flexibly, realized full unit, global function, the strong robotization of coordinating, make this instrument be suitable for industry online, at a high speed, high precision, big concentration difference analytical test.
Description of drawings: the present invention is further made an explanation below in conjunction with accompanying drawing.
Fig. 1 is the structural principle synoptic diagram of embodiments of the invention.
Electronic multiple valve sample states synoptic diagram when Fig. 2 is the constant sample mode.
Electronic multiple valve when Fig. 3 is the constant sample mode is annotated the sample view.
Electronic multiple valve sample states synoptic diagram when Fig. 4 is the trace sample mode.
Electronic multiple valve when Fig. 5 is the trace sample mode is annotated the sample view.。
Embodiment: as shown in Figure 1: high-speed automatic colorimetric analyzer embodiment, be by adopting automatically/impregnator, the all unit of colour developing/colour comparator connect composition in regular turn, and gather Various types of data (data acquisition is represented with the dotted line of " coming to arrow ") respectively from above-mentioned all unit by single-chip microcomputer 4, the control analysis instrument carries out colorimetric analysis automatically after the data processing, show (control relation is represented with the dotted line of " whereabouts arrow "), wherein output/input peripheral 4-1 realizes data presentation and man-machine communication, it is characterized in that: described adopting automatically/impregnator, it is the electronic multiple valve 1 that adopts eight channel-types, its valve opening is divided into three groups, wherein A group valve opening is connected three respectively respectively in order to contain sample liquid, the liquid bath A1 of reagent and boosting liquid (or eluent), A2, A3, high precision piston pump B1 of B group each self-closing of valve opening, B2, B3, C group valve opening C1, C2 converges the inlet 2 that uncovered colour developing pond 6 is connected in the back, and certain is to valve opening C3, be serially connected with sampling ring 3 between C4.
Described single-chip microcomputer 4 be provided with photometric signal to adopt, the control loop of injection amount, can regulate feed liquor amount and reservoir quantity according to the described high precision piston pump B1 of 5 controls of the luminosity value in the colour developing/colour comparator, B2, B3 to sample liquid, reagent and boosting liquid (or eluent).
Described colour developing/colour comparator is that the microspectrophotometer 7 by discrete each other uncovered colour developing pond 6 and band flow cell is composed in series by infusion pipeline.
Described uncovered colour developing pond 6 open topped are communicated with atmosphere, periphery is provided with temperature-sensitive head 8-0 and temperature control well heater 8, its underpart is provided with magnetic stirrer 9, the inlet of opening 2 by threeway with adopt automatically/liquid injection port C1, C2 and a peristaltic pump 12 of impregnator be connected, its liquid outlet 10 is connected with flow cell inlet 11 and distillation tank 19 in the microspectrophotometer 7 respectively by three-way solenoid valve 18; The center of the microspectrophotometer 7 of described band flow cell is a flow cell 13, the inlet 11 of flow cell 13 is connected with the three-way solenoid valve 18 of 6 bottoms, uncovered colour developing pond, outlet 14 is connected with a peristaltic pump 15, and is respectively equipped with monochromatic collimated beam source 16 and photoelectric commutator 17 in transparent flow cell 13 relative both sides.
Described high precision piston pump B1, B2, B3 are made of the syringe that metering stepper motor (by illustrating) drives, and its measuring accuracy of adopting, annotating sample reaches the 0.87 μ l/ step.
As shown in Figure 2: the valve opening stream of electronic multiple valve 1, by being close to and can two valve block stator 1-1 and rotor 1-2 (all launching expression) institute form in relative rotation around axle, horizontal line among stator 1-1 and the rotor 1-2 is represented the valve block internal path, the sealing of cross " * " expression path, the solid arrow express liquid flows to (down together).On stator 1-1, high precision piston pump B1, valve opening that B3 connects are adjacent in turn, and the C4 of the sampling ring 3 end institute valve opening that connects is alternate in turn with valve opening that B3 connects, high precision piston pump valve opening that B2 connects again with C4 hold the valve opening that connects alternate in turn; On rotor 1-2, sampling ring 3 holds valve opening that C3 connects and fluid injection stream C1, valve opening that C2 connects alternate in turn in addition, feed liquor stream A3, A1, valve opening that A2 connects are also alternate in turn, and C3 and fluid injection stream C1, valve opening that C2 connects are adjacent in turn with feed liquor stream A3, A1, valve opening that A2 connects again; When doing the constant sampling, sample liquid concentration need not enrichment, the rotor 1-2 of the electronic multiple valve 1 of single-chip microcomputer 4 control rotates and switches: with high precision piston pump B1, sampling ring 3 and boosting liquid (eluent) groove A1 is vacant need not, and high precision piston pump B3, B2 are docked and connected with feed liquor stream A3, A2 respectively, and drive stepping motor (not shown) drive high precision piston pump B3, B2 measure drawing liquid to sample liquid and reagent in two grooves respectively.
Shown in Fig. 3,1: above-mentioned metering, drawing liquid action are finished, when changing constant notes sample over to, the rotor 1-2 of the electronic multiple valve 1 of single-chip microcomputer 4 controls rotates and switches: high precision piston pump B2 and fluid injection stream C2 are docked and connected, the C3 end of high precision piston pump B3 and sampling ring 3 is docked and connected, the C4 of while sampling ring 3 holds just and fluid injection stream C1 is docked and connected, and drive stepping motor (not shown) drive high precision piston pump B3, B2 do to measure fluid injections to uncovered colour developing pond 6 bottom inlets 2 respectively; This moves simultaneously, single-chip microcomputer 4 control peristaltic pumps 12 are closed and whole uncovered colour developing pond 6 systems heat up, stir, colour developing, and control three-way solenoid valve 18 is closed distillation tank 19 pipelines, and the inlet 11 of flow cell 13 is connected with the liquid outlet 10 in uncovered colour developing pond 6, control peristaltic pump 15 6 extracts colour developing sample liquid are accepted monochromatic collimated beam source 16 and photoelectric commutator 17 to flow cell 13 colorimetric detection from uncovered colour developing pond, then, single-chip microcomputer 4 is to output/input peripheral 4-1 output testing result and record, then, single-chip microcomputer 4 drives the peristaltic pump 12 at inlet 2 places and exports the peristaltic pump 15 emptying raffinates at 14 places, cooperate the three-way solenoid valve 18 extraction distilled water at inlet 11 places to clean two ponds and pipeline, and enter the analysis cycle of next round sample.
As shown in Figure 4: high-speed automatic colorimetric analyzer of the present invention, when doing the trace sampling, sample liquid concentration is extremely low, fill enriching column in the sampling ring 3, all be same as the constant sampling in the C4 of high precision piston pump B1, B2 on the stator 1-1, B3, sampling ring 3 end, feed liquor stream A1, A2, A3, fluid injection stream C1, the C2 on rotor 1-2, the C4 end institute valve opening that the connects relation of sampling ring 3, contain sample liquid among the A1 but change into, contain eluent among the A3; At this moment, the rotor 1-2 of the electronic multiple valve 1 of single-chip microcomputer 4 controls rotates and switches: the feed liquor stream of high precision piston pump valve opening that B3 connects and eluant groove A3 is docked and connected, the C3 end of high precision piston pump valve opening that B1 connects and sampling ring 3 is docked and connected, the C4 end of sampling ring 3 is docked and connected with sample liquid bath A1 feed liquor stream, the feed liquor stream of high precision piston pump valve opening that B2 connects and reagent trough A2 is docked and connected, and the drive stepping motor (not shown) drives high precision piston pump B1, B2, B3 is respectively from A1, A2, A3 measures drawing liquid, confirms the extraction amount of each liquid up to single-chip microcomputer 4.
As Fig. 5, shown in 1: above-mentioned metering, the drawing liquid action is finished, when changing trace notes sample over to, the rotor 1-2 of the electronic multiple valve 1 of single-chip microcomputer 4 controls rotates and switches: make unnecessary sample liquid in the high precision piston pump B1 blowdown pump, high precision piston pump valve opening that B2 connects and fluid injection stream C2 are docked and connected, B3 the C3 end of the valve opening that connects and sampling ring 3 be docked and connected, and the C4 end valve opening that connects and the fluid injection stream C1 of sampling ring 3 are docked and connected, then, single-chip microcomputer 4 drive stepping motor (not shown) drive high precision piston pump B3 and release pumped storage eluent wherein, test substance wash-out with enrichment in the enriching column of sampling ring 4, inject uncovered colour developing pond 6 through inlet 2, and make the reagent among the high precision piston pump B2 also in uncovered colour developing pond 6, do the metering fluid injection respectively through inlet 2; This moves simultaneously, single-chip microcomputer 4 control peristaltic pumps 12 are closed and whole uncovered colour developing pond 6 systems heat up, stir, colour developing, and control three-way solenoid valve 18 is closed distillation tank 19 pipelines, and the inlet 11 of flow cell 13 is connected with the liquid outlet 10 in uncovered colour developing pond 6, control peristaltic pump 15 6 extracts colour developing sample liquid are accepted monochromatic collimated beam source 16 and photoelectric commutator 17 to flow cell 13 colorimetric detection from uncovered colour developing pond, if photometric signal is not obvious, single-chip microcomputer 4 will drive accent such as high precision piston pump B1 and increase sample liquid and extract and reservoir quantity, up to suitable shading value occurring, then, single-chip microcomputer 4 is to output/input peripheral 4-1 output testing result and record, then single-chip microcomputer 3 drives the peristaltic pump 12 at inlet 2 places again and exports the peristaltic pump 15 emptying raffinates at 14 places, cooperate the three-way solenoid valve 18 extraction distilled water at inlet 11 places to clean two ponds and pipeline, and enter the analysis cycle of next round sample.
For the analysis of the high sample liquid of testing concentration, only need to use the sampling ring 3 of hollow, and change eluent into boosting liquid, get final product according to the similar process analysis of Fig. 4, Fig. 5.
Claims (6)
1, a kind of high-speed automatic colorimetric analyzer, by adopt automatically/impregnator, all unit of colour developing/colour comparator connect in regular turn and form, and gather Various types of data respectively from above-mentioned all unit by control system, the control analysis instrument carries out colorimetric analysis, demonstration automatically after the data processing, it is characterized in that: described adopting automatically/impregnator, be to adopt electronic multiple valve, as the coupling arrangement of high precision piston pump butt joint feed tube, liquid injection pipe and adopt the switching device shifter of liquid, fluid injection state.
2, high-speed automatic colorimetric analyzer as claimed in claim 1 is characterized in that: described electronic multiple valve is eight or 12 channel-types; Its valve opening is divided into three groups, wherein A group valve opening is connected sample liquid bath, reagent trough, boosting liquid (or eluent) groove respectively, high precision piston pump of B group each self-closing of valve opening, C group valve opening converges the back and connects colour developing/colour comparator inlet, and certain sampling ring to being serially connected with hollow between valve opening or filling enriching column.
3, as arbitrary described high-speed automatic colorimetric analyzer in the claim 1,2, it is characterized in that: described control system is made up of single-chip microcomputer and output/input peripheral thereof, it is provided with photometric signal to adopting the control loop of liquid, reservoir quantity, can control described high precision piston pump according to the luminosity value in the colour developing/colour comparator, realize sample liquid, reagent and the collection of boosting liquid (or eluent), the adjusting of injection rate IR.
4, high-speed automatic colorimetric analyzer as claimed in claim 3 is characterized in that: described colour developing/colour comparator is to be composed in series by infusion pipeline by the microspectrophotometer of discrete each other uncovered colour developing pond with the band flow cell.
5, high-speed automatic colorimetric analyzer as claimed in claim 4, it is characterized in that: described uncovered colour developing pond open topped is communicated with atmosphere, periphery is provided with the temperature control well heater, its underpart is provided with magnetic stirrer, institute's inlet of opening by threeway with adopt automatically/impregnator and a peristaltic pump be connected, its liquid outlet by three-way solenoid valve respectively with microspectrophotometer in the flow cell inlet and distill tank and be connected; The center of the microspectrophotometer of described band flow cell is a flow cell, the flow cell inlet is connected with the three-way solenoid valve of bottom, uncovered colour developing pond, outlet is connected with a peristaltic pump, and is respectively equipped with monochromatic collimated beam source and photoelectric commutator in the relative both sides of flow cell.
6, high-precision fully automatic photomatrix titrimetric analysis instrument as claimed in claim 5 is characterized in that: described high precision piston pump is made of the stepper motor driven syringe of metering, and its measuring accuracy of adopting, annotating sample reaches the 0.87 μ l/ step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01131524 CN1346984A (en) | 2001-11-07 | 2001-11-07 | High-speed automatic colorimetric analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01131524 CN1346984A (en) | 2001-11-07 | 2001-11-07 | High-speed automatic colorimetric analyzer |
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| CN1346984A true CN1346984A (en) | 2002-05-01 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101793839B (en) * | 2010-02-08 | 2011-11-23 | 江苏德林环保技术有限公司 | Colorimetric detection method and device of sugar content of water |
| CN102258885A (en) * | 2011-05-18 | 2011-11-30 | 中国地质大学（武汉） | Automatic separation system for integrated column chromatography |
| CN103278655A (en) * | 2013-05-23 | 2013-09-04 | 南京工业大学 | Flow path system suitable for glycosylated hemoglobin analysis based on multichannel peristaltic pump |
| CN108645844A (en) * | 2018-01-22 | 2018-10-12 | 河海大学 | A kind of automation solution chromogenic reaction sensor |
| CN110320166A (en) * | 2018-03-29 | 2019-10-11 | 株式会社岛津制作所 | A kind of judgment method judging whether there is test sample supply |
-
2001
- 2001-11-07 CN CN 01131524 patent/CN1346984A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101793839B (en) * | 2010-02-08 | 2011-11-23 | 江苏德林环保技术有限公司 | Colorimetric detection method and device of sugar content of water |
| CN102258885A (en) * | 2011-05-18 | 2011-11-30 | 中国地质大学（武汉） | Automatic separation system for integrated column chromatography |
| CN103278655A (en) * | 2013-05-23 | 2013-09-04 | 南京工业大学 | Flow path system suitable for glycosylated hemoglobin analysis based on multichannel peristaltic pump |
| CN103278655B (en) * | 2013-05-23 | 2014-06-11 | 南京工业大学 | Flow path system suitable for glycosylated hemoglobin analysis based on multichannel peristaltic pump |
| CN108645844A (en) * | 2018-01-22 | 2018-10-12 | 河海大学 | A kind of automation solution chromogenic reaction sensor |
| CN110320166A (en) * | 2018-03-29 | 2019-10-11 | 株式会社岛津制作所 | A kind of judgment method judging whether there is test sample supply |
| CN110320166B (en) * | 2018-03-29 | 2022-08-12 | 株式会社岛津制作所 | Judgment method for judging whether detection sample is supplied or not |
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