CN1343729A - Liquorice polyose - Google Patents
Liquorice polyose Download PDFInfo
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- CN1343729A CN1343729A CN00124539A CN00124539A CN1343729A CN 1343729 A CN1343729 A CN 1343729A CN 00124539 A CN00124539 A CN 00124539A CN 00124539 A CN00124539 A CN 00124539A CN 1343729 A CN1343729 A CN 1343729A
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- centrifugal
- polysaccharide
- precipitating thing
- water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Abstract
A liquorice polyose for improving immunity and suppressing tumor and AIDS is prepared from the root and stem of liquorice through cutting, boiling in water at 100 deg.C for 7-8 hrs, extracting supernatant, decolouring, concentrating, deposition in alcohol, centrifugal separation, baking, grinding, sieving to obtain coarse polyose, adding water, filtering under 4 deg.C, centrifugal separation to obtain supernatant, adding sevage reagent to remove protein, dialysis with deionic water, chromatography by DEAE-Cellulose (DE-32) column, educting, concentrating, deposition in alcohol, centrifugal separation, washing with alcohol and vacuum drying.
Description
The invention belongs to the extract that derives from vegetable material Chinese medicine, particularly from Radix Glycyrrhizae, the polysaccharide material that the efficient part purification forms.Be a kind of relevant treatment tumour that relates to, strengthen immune function of human body, the licorice polysaccharide of better curative effect is arranged suppressing AIDS.
At present, tumour is common frequently-occurring disease, and this disease does not still have specifics in the western medicine at home and abroad, and the drug price costliness, makes most of patients give birth to fear.In recent years, China's Chinese traditional treatment tumor research is very active, has all obtained certain progress from the basic theory to the clinical treatment.Report that from all parts of the country curative effect differs, benevolence intelligence is respectively seen.How improving clinical efficacy for this reason, visit and get the treatment rule, is the task of top priority.To the treatment of AIDS, also do not have both at home and abroad and find especially effectively medicine.
The objective of the invention is, provide a kind of mediator's body immunity function to suppress the licorice polysaccharide of tumour and AIDS.Prove through a large amount of scientific experiments, this product can not only activating macrophage, the TB lymphocyte, thereby strengthen the dual-use function of humoral immunization and cellular immunization, simultaneously, licorice polysaccharide is to reaching inhibited to the reversed transcriptive enzyme of AIDS from the effect of tumour cell telomere, make genome (DNA or RNA), can't normal replication, thus play inhibition or directly deactivation to virus or tumour, be the purpose of improving the general immunity function and more effectively suppressing tumour and AIDS.
The present invention is a kind of synthetic drugs that is different from, and immune function of human body suppresses tumour and AIDS is the natural green medicine of purpose to strengthen.
The present invention is achieved in that the preparation process of licorice polysaccharide is as follows: one, raw material is handled extracting liquorice root, stem cutting, water flushing impurity elimination.Two, polysaccharide prepares 1. raw materials and adds water, and 100 ℃ were boiled 7～8 hours, filtered out impurities, and obtained clear liquid.2. through activated carbon decolorizing.3. destainer concentrates, and uses alcohol precipitation.4. the centrifugal precipitating thing that obtains.5. Crude polysaccharides is dried, ground, sieves, obtains to the precipitating thing.6. Crude polysaccharides adds water, and 4 ℃ are filtered swelling.7. centrifugal, get supernatant liquor.8. add Sevage reagent and remove albumen.9. dialyse with deionized water.10. through DEAE-Cellulose (DE-32) column chromatography.11. elutriant concentrates.12. use the alcohol precipitation polysaccharide.13. centrifugal, obtain the precipitating thing.14. with alcohol washing 2 times.15. vacuum-drying.16. grind to form the white powder polysaccharide.17. putting shady and cool dry place preserves.Three, packing 1. is packed white powder polysaccharide powder in the ampere bottle into, and every bottle of 1mg sterilizes through physical method.2. be packaged into box, envelope is stabbed.
Characteristics of the present invention are: the physics and chemistry of licorice polysaccharide and biological characteristics 1. physicochemical properties:
(1) nonnitrogenous, no conjugated protein, main ingredient is the dextran of glucose.
(2) [2] D
28:+120 ° of (cO.1, H
2O) specific optical rotation.
(3) Infrared spectroscopy, absorption peak (cm
-1) 855～833 be 2-D Portugal pyrans
Sugar, licorice polysaccharide tool 850.6230 absorption peaks show that it belongs to the 2-D-pyrans
Dextran.
(4), be indicated as the dextran that 2-(1.4) key connects through nuclear magnetic resonance measuring.
(5) through methylating and mass spectroscopy, its molecular weight is that 37000 Dalton molecular structures are 2-D-Glep-(1
→4)-2-D-Glep-(1
→4)
226-2-D-Glep
(6) colourless, tasteless powdery substance.
(7) soluble in water.2. biological characteristics
(1) can reach 85% to entity pain S-180 tumour inhibiting rate.(2) the macrophage phagocytic rate can improve 66%.(3) adenovirus III type cells infected protection ratio is improved 74.5%, very viral to Ke gallery
The cells infected protection ratio improves 59.1%.(4) reach 36.2% for the HIV (human immunodeficiency virus) inhibiting rate.
Prove through a large amount of scientific experiments, this product can not only activating macrophage, the TB lymphocyte, thereby strengthen the dual-use function of humoral immunization and cellular immunization, simultaneously, licorice polysaccharide is to reaching inhibited to the reversed transcriptive enzyme of AIDS from the effect of tumour cell telomere, make genome (DNA or RNA), can't normal replication, thus play inhibition or directly deactivation to virus or tumour, be the purpose of improving the general immunity function and more effectively suppressing tumour and AIDS.
Fig. 1 is that the Crude polysaccharides preparation technology figure of licorice polysaccharide preparation technology schema: Fig. 2 is the holosaccharide preparation technology figure of licorice polysaccharide preparation technology schema
This product is confirmed innocuous substance through the toxicological assessment examining report. (seeing Appendix 1).
This product is confirmed stay in grade through functional component, the stability test of health products. (see Appendix 2).
This product has inhibitory action and directly deactivation through the antagonism virus research, to macrophage Phagocytic function research has obvious facilitation that immunologic enhancement is arranged, to the tumor-inhibiting action test as seen The karyopycnosis of large stretch of degeneration necrosis cell and the property made distribution. (seeing Appendix 3).
This product shows through antagonism AIDS drug test report: virus replication is had certain inhibition Effect is 36.2% to viral inhibiting rate. (seeing Appendix 4)
Claims (2)
1, a kind of licorice polysaccharide is characterized in that: obtained by following preparation method's process, the root of extracting liquorice, stem cut into fritter, water flushing decon; Add entry, heat 100 ℃ and boiled 7～8 hours, filter out impurities, extract clear liquid; Again through activated carbon decolorizing; Liquid after the decolouring concentrates, and uses alcohol precipitation again; Adopt centrifugal method to obtain the precipitating thing; Again the precipitating thing is dried, is ground, sieves, obtains Crude polysaccharides; Add water by Crude polysaccharides again, filter swelling down for 4 ℃ in temperature; After centrifugal, get supernatant liquor again; Add Sevage reagent again and remove Deproteinization; Dialyse with deionized water again; Again through DEAE-Cellulose (DE-32) column chromatography; Concentrate through elutriant again, use the alcohol precipitation polysaccharide, again through centrifugal acquisition precipitating thing; Again with alcohol washing 2 times; Through vacuum-drying, grind, the material that obtains white powder is licorice polysaccharide again.
2, licorice polysaccharide according to claim 1, its preparation method is: the root of extracting liquorice, stem cut into fritter, water flushing decon; Add entry, heat 100 ℃ and boiled 7～g hour, filter out impurities, extract clear liquid; Again through activated carbon decolorizing; Liquid after the decolouring concentrates, and uses alcohol precipitation again; Adopt centrifugal method to obtain the precipitating thing; Again the precipitating thing is dried, is ground, sieves, obtains Crude polysaccharides; Add water by Crude polysaccharides again, filter swelling down for 4 ℃ in temperature; After centrifugal, get supernatant liquor again; Add Sevage reagent again and remove Deproteinization; Dialyse with deionized water again; Again through DEAE-Cellulose (DE-32) column chromatography; Concentrate through elutriant again, use the alcohol precipitation polysaccharide, again through centrifugal acquisition precipitating thing; Again with alcohol washing 2 times; Through vacuum-drying, grind again, obtain the white powder licorice polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00124539A CN1343729A (en) | 2000-09-20 | 2000-09-20 | Liquorice polyose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00124539A CN1343729A (en) | 2000-09-20 | 2000-09-20 | Liquorice polyose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1343729A true CN1343729A (en) | 2002-04-10 |
Family
ID=4590488
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|---|---|---|---|
| CN00124539A Pending CN1343729A (en) | 2000-09-20 | 2000-09-20 | Liquorice polyose |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310952C (en) * | 2004-06-04 | 2007-04-18 | 山东鲁抗集团中澳生物科技有限公司 | Extraction of polysaccharose of three bristle cudrania |
| CN100404526C (en) * | 2006-01-20 | 2008-07-23 | 华中科技大学 | Method for extracting glycyrrhizicacid, licorice flavone and licorice polysaccharide |
| CN100432232C (en) * | 2005-04-30 | 2008-11-12 | 黑龙江红豆杉药业有限责任公司 | Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use |
| CN101525391B (en) * | 2009-03-31 | 2011-05-11 | 武汉大学 | Method for extracting amylase from liquorice and application thereof |
| CN102311509A (en) * | 2011-06-17 | 2012-01-11 | 青阳县杰灵生物工程有限责任公司 | Extracting method of sweet potato stem leaf polysaccharide |
| CN102532337A (en) * | 2011-12-29 | 2012-07-04 | 天津大学 | Method for producing glycyrrhizia polysaccharide by utilizing tissue culture method |
| CN103864952A (en) * | 2014-04-02 | 2014-06-18 | 天津中医药大学 | Preparation method and application of micro-molecular glycyrrhiza polysaccharide with anti-tumor activity |
| CN107987176A (en) * | 2017-11-29 | 2018-05-04 | 马斌祥 | A kind of technique using radix glycyrrhizae extraction high-purity liquorice polysaccharide |
| CN108191988A (en) * | 2017-12-26 | 2018-06-22 | 浦江县美泽生物科技有限公司 | Licorice polysaccharide extraction process |
| CN110343188A (en) * | 2019-07-30 | 2019-10-18 | 中国人民解放军总医院第五医学中心 | A kind of method for extraction and purification of licorice polysaccharide |
-
2000
- 2000-09-20 CN CN00124539A patent/CN1343729A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310952C (en) * | 2004-06-04 | 2007-04-18 | 山东鲁抗集团中澳生物科技有限公司 | Extraction of polysaccharose of three bristle cudrania |
| CN100432232C (en) * | 2005-04-30 | 2008-11-12 | 黑龙江红豆杉药业有限责任公司 | Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use |
| CN100404526C (en) * | 2006-01-20 | 2008-07-23 | 华中科技大学 | Method for extracting glycyrrhizicacid, licorice flavone and licorice polysaccharide |
| CN101525391B (en) * | 2009-03-31 | 2011-05-11 | 武汉大学 | Method for extracting amylase from liquorice and application thereof |
| CN102311509A (en) * | 2011-06-17 | 2012-01-11 | 青阳县杰灵生物工程有限责任公司 | Extracting method of sweet potato stem leaf polysaccharide |
| CN102532337A (en) * | 2011-12-29 | 2012-07-04 | 天津大学 | Method for producing glycyrrhizia polysaccharide by utilizing tissue culture method |
| CN103864952A (en) * | 2014-04-02 | 2014-06-18 | 天津中医药大学 | Preparation method and application of micro-molecular glycyrrhiza polysaccharide with anti-tumor activity |
| CN107987176A (en) * | 2017-11-29 | 2018-05-04 | 马斌祥 | A kind of technique using radix glycyrrhizae extraction high-purity liquorice polysaccharide |
| CN108191988A (en) * | 2017-12-26 | 2018-06-22 | 浦江县美泽生物科技有限公司 | Licorice polysaccharide extraction process |
| CN110343188A (en) * | 2019-07-30 | 2019-10-18 | 中国人民解放军总医院第五医学中心 | A kind of method for extraction and purification of licorice polysaccharide |
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