CN1343523A - Millipore non-cell xanoepidermis substitute - Google Patents
Millipore non-cell xanoepidermis substitute Download PDFInfo
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- CN1343523A CN1343523A CN 00125272 CN00125272A CN1343523A CN 1343523 A CN1343523 A CN 1343523A CN 00125272 CN00125272 CN 00125272 CN 00125272 A CN00125272 A CN 00125272A CN 1343523 A CN1343523 A CN 1343523A
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- acellular dermis
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Abstract
A millipore non-cell xenoepidermis substitute used for repairing wound is prepared through regular and dense perforation on whole epidermis by mechanical method or CO2 laser. Its advantages include high nutrients permeability, high viral rate and no fluid, air or blood collection.
Description
The present invention relates to medical domain, particularly a kind of micropore allosome (kind) acellular dermis substitute that is used for wound repair.
At present, substituting one of damaged skin corium, raising wound healing method for quality in degree of depth skin injury wound repair is the acellular dermis substitute of transplanting allosome (kind).The acellular dermis substitute is meant to be handled people's cadaver skin or animal (pig) skin through enzymic digestion, detergent, remove the strong cell composition of antigenicity, and keep the dermis scaffold of collagen bundle and basement membrane structure.This kind substitute no antigen, bioaffinity is good, but the inducing self-body Interstitial cell soaks into growth, rebuilds skin corium after implanting wound surface.Acellular dermis improves the wound healing quality in conjunction with can finally repairing degree of depth skin injury wound surface from body skin cograft.But allosome (kind) the acellular dermis substitute quality of using is fine and close at present, implant wound surface substrate blood plasma isotonic solution behind the wound surface be difficult to see through skin corium nutrition its go up covering from the body skin, this kind acellular dermis needs the ability vascularization of 1～2 week after implanting wound surface on the other hand, these factors all cause being covered in is transplanting early stage and the later stage all is difficult to obtain the sufficient nutrition supply from the body skin on the acellular dermis, thereby a little less than the anti-infection ability, survival rate is low.And the employing technology of drawing in the net commonly used clinically at present, acellular dermis was drawn in the net (1: 1,1: 2 etc.) back implantation wound surface, although will or will stagger from the netted skin of body and acellular dermis mesh and can improve survival rate from body sword pachydermia pressure dressing, but behind the wound healing, form point-like, netted cicatrix inevitably at acellular dermis mesh position, reduced the effect of acellular dermis as dermis scaffold.
The purpose of this invention is to provide that a kind of nutrition permeability is strong, vascularization speed is fast, can improve the self-skin transplant survival rate, do not influence micropore allosome (kind) the acellular dermis substitute of dermis scaffold effect simultaneously.
The present invention is the improvement that at present used clinically acellular dermis substitute and acellular dermis are drawn in the net, on the acellular dermis substitute of quality densification, stamp micropore, make wound surface substrate blood plasma isotonic solution can see through micropore nutrition its go up covering from the body skin, and be beneficial to fibroblast, vascular endothelial cell and soak into growth, form vessel pedicle, quicken vascularization, improve survival rate; And, overcome the defective of mesh position formation point-like, netted cicatrix after acellular dermis draws in the net because micropore size is little.
The method preparation that the present invention adopts conventional enzymic digestion, detergent to soak derives from the cell-less corium ground substance of cadaver skin, Corii Sus domestica, and thickness is 0.2mm～0.8mm.Punch in the penetrability of the intensive rule of the surperficial row of whole acellular dermis then.For guaranteeing that hole can absorb wound exudate and be beneficial to angiogenic growth by siphonage, pore size is at 50um～2mm, and pitch of holes is 1mm～1cm.The method of punching is divided into two classes: (1) mechanical punching: adopt mechanical or manual method, with the fine needle of corresponding size, with certain pitch of holes row penetrability punching.(2) laser boring: utilize the non-specific heat effect principle of laser, use CO
2Laser is with certain power, distance rule irradiation acellular dermis, and until the carbonization of corium point-like, perforation, it is standby to clean at last, pack, sterilize.Select suitable model to be cut into the wound surface size during use, with the acellular dermis substitute corium implantation wound surface that faces down, suitable sutured.(0.2mm～0.5mm) can cover immediately from the body skin, postoperative 2～3 all wound surface can heal for thin acellular dermis.And (0.51mm～0.8mm) treats after the vascularization in 3～4 days can to improve autologous transplanting skin again survival rate and to reach more than 95% when implanting thicker acellular dermis.
Because the present invention makes intensive micropore acellular dermis substitute with the acellular dermis of common densification, compares with the acellular dermis substitute of atresia, has following remarkable advantage:
1. the small and dense collection of the hole of acellular dermis substitute of the present invention, behind its implantation wound surface, can make wound exudate be penetrated into the acellular dermis surface by the capillary siphoning effect, guarantee on it to cover from the body skin transplanting the early stage sufficient nutrition supply that obtains, improve survival rate;
2. Interstitial cell such as fibroblast, vascular endothelial cell can take the lead in by micropore, forms the vessel pedicle spline structure along collagen stroma, quickens vascularization, makes from the body skin and is transplanting the supply of later stage acquisition adequate blood, raising survival rate and anti-infection ability;
3. dense micropore can prevent acellular dermis substitute transplanting back hypohydrops, pneumatosis, hematocele;
4. acellular dermis substitute of the present invention aperture is little, if with from body sword pachydermia, netted skin, stamp skin or microparticle skin cograft, can not form point-like or netted cicatrix, do not influence outward appearance and function behind the wound healing.Overcome acellular dermis has been drawn in the net afterwards to transplant the easily defective of formation point-like, netted cicatrix.
The present invention is further illustrated below in conjunction with embodiment
Embodiment 1:
The thick about 0.8mm of xenogenesis (pig) acellular dermis, long 10cm, wide 10cm.Produce JZ-30A type CO with Beijing
2Laser (cutter head is apart from acellular dermis 0.5cm for wavelength 10.6um, output 18W) burns corium to point-like carbonization perforation, and the aperture is 100um, and pitch of holes is 1.5mm.Cleaning the back packs, sterilizes standby.
Embodiment 2:
Allosome acellular dermis thickness 0.6mm, long 15cm, wide 15cm is with CO
2The intensive punching of laser, aperture are 300um, and pitch of holes is 2.5mm.Cleaning the back packs, sterilizes standby.
Embodiment 3:
Allosome acellular dermis thickness 0.4mm, long 10cm, wide 20cm.Method with craft is punched the about 5mm of pitch of holes with the fine steel needle that diameter is about 1mm.Cleaning the back packs, sterilizes standby.
Micropore acellular dermis substitute of the present invention can be used for wound repair behind deep burn, multiple chronic skin ulcer and the scar excision.Show through a large amount of animal (SD rat) experiments and part clinic trial, will (0.2mm～0.5mm) implants holostrome skin injury wound surface than thin micropore acellular dermis substitute, adopt a step grafting to cover immediately from body sword pachydermia, netted skin, stamp skin, microparticle skin, can guarantee at the nutrition supply of transplanting early stage and later stage from the body skin, survival rate brings up to 90% by 75%, and the while has been avoided acellular dermis to draw in the net afterwards again and easily formed the defective of point-like, netted cicatrix from body skin cograft.(0.51mm～0.8mm), the vascularization time adopts two step graftings can reach more than 95% from body skin survival rate by shortening to 3～4 days 1～2 week and transplant thicker micropore acellular dermis substitute.
Claims (2)
1. a micropore allosome (kind) acellular dermis substitute is characterized in that the acellular dermis substitute has the intensive penetrability micropore of rule.
2. by the described micropore allosome of claim 1 (kind) acellular dermis substitute, it is characterized in that described intensive penetrability micropore size size is 50um～2mm, pitch of holes is 1mm～1cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001252720A CN1176725C (en) | 2000-09-19 | 2000-09-19 | Millipore non-cell xanoepidermis substitute |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001252720A CN1176725C (en) | 2000-09-19 | 2000-09-19 | Millipore non-cell xanoepidermis substitute |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1343523A true CN1343523A (en) | 2002-04-10 |
| CN1176725C CN1176725C (en) | 2004-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001252720A Expired - Fee Related CN1176725C (en) | 2000-09-19 | 2000-09-19 | Millipore non-cell xanoepidermis substitute |
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| CN (1) | CN1176725C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829360A (en) * | 2010-04-16 | 2010-09-15 | 中国人民解放军第二军医大学 | Method for preparing acellular ligament or tendon stent |
| CN101856516A (en) * | 2010-05-25 | 2010-10-13 | 中国人民解放军总医院第一附属医院 | Preparation of collagen-chitosan-laser micropore dermal matrix composite membranes |
| CN101985052A (en) * | 2010-08-31 | 2011-03-16 | 中国人民解放军第二军医大学 | Skin substitute for automatically capturing endothelial progenitor cells and promoting vascularization and construction method thereof |
| CN102188751A (en) * | 2010-03-19 | 2011-09-21 | 温州医学院附属第一医院 | Laser micropore porcine acellular dermal matrix and preparation method thereof |
| CN104288837A (en) * | 2014-09-11 | 2015-01-21 | 杨淑珍 | Acellular dermal matrix and preparation method and application thereof |
| CN105688286A (en) * | 2014-08-08 | 2016-06-22 | 罗旭 | Laser micro-drilled acellular dermal matrix and preparation method thereof |
| CN109045357A (en) * | 2018-06-11 | 2018-12-21 | 武汉奥翔生物科技有限公司 | Provenance skin and preparation method thereof |
| CN109701077A (en) * | 2019-01-29 | 2019-05-03 | 北京颢美细胞基因生物技术有限公司 | A kind of micropore regenerating tissues matrix and its preparation and application |
-
2000
- 2000-09-19 CN CNB001252720A patent/CN1176725C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102188751A (en) * | 2010-03-19 | 2011-09-21 | 温州医学院附属第一医院 | Laser micropore porcine acellular dermal matrix and preparation method thereof |
| CN101829360B (en) * | 2010-04-16 | 2013-04-03 | 中国人民解放军第二军医大学 | Method for preparing acellular ligament or tendon stent |
| CN101829360A (en) * | 2010-04-16 | 2010-09-15 | 中国人民解放军第二军医大学 | Method for preparing acellular ligament or tendon stent |
| CN101856516A (en) * | 2010-05-25 | 2010-10-13 | 中国人民解放军总医院第一附属医院 | Preparation of collagen-chitosan-laser micropore dermal matrix composite membranes |
| CN101856516B (en) * | 2010-05-25 | 2011-11-30 | 中国人民解放军总医院第一附属医院 | Preparation of collagen-chitosan-laser micropore dermal matrix composite membranes |
| CN101985052B (en) * | 2010-08-31 | 2013-11-06 | 中国人民解放军第二军医大学 | Skin substitute for automatically capturing endothelial progenitor cells and promoting vascularization and construction method thereof |
| CN101985052A (en) * | 2010-08-31 | 2011-03-16 | 中国人民解放军第二军医大学 | Skin substitute for automatically capturing endothelial progenitor cells and promoting vascularization and construction method thereof |
| CN105688286A (en) * | 2014-08-08 | 2016-06-22 | 罗旭 | Laser micro-drilled acellular dermal matrix and preparation method thereof |
| CN104288837A (en) * | 2014-09-11 | 2015-01-21 | 杨淑珍 | Acellular dermal matrix and preparation method and application thereof |
| CN109045357A (en) * | 2018-06-11 | 2018-12-21 | 武汉奥翔生物科技有限公司 | Provenance skin and preparation method thereof |
| CN109321514A (en) * | 2018-06-11 | 2019-02-12 | 武汉奥翔生物科技有限公司 | Humanization skin and preparation method thereof |
| CN109701077A (en) * | 2019-01-29 | 2019-05-03 | 北京颢美细胞基因生物技术有限公司 | A kind of micropore regenerating tissues matrix and its preparation and application |
| CN109701077B (en) * | 2019-01-29 | 2021-07-30 | 北京颢美细胞基因生物技术有限公司 | Micropore regeneration tissue matrix and preparation and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1176725C (en) | 2004-11-24 |
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