CN210301825U - 3D prints skin - Google Patents

Abstract

The utility model provides a 3D prints skin, this 3D prints skin include the skin layer that 3D printed and made, still include a plurality of micropins, the micropin imbeds in the skin layer, the first end of micropin is worn to or is passed the surface of skin layer, the second end of micropin is close to, is worn to or is passed the bottom surface of skin layer; the microneedle comprises at least one passageway disposed between a first end and a second end. The utility model discloses a 3D prints skin and laminates completely and has the material transport function with the impaired position of human skin.

Description

3D prints skin

Technical Field

The utility model relates to a 3D prints and tissue engineering biomedical material field, specifically relates to a 3D prints skin.

Background

The skin is the largest organ of the human body, and not only has the function of resisting external environmental infection, but also plays a role in preventing water, electrolytes and other substances in the body from being lost. In general, skin can recover itself if it is mildly damaged. When a large area of skin is severely damaged, such as a burn, the doctor must immediately introduce fluid and protect the wound. If only the superficial layer of skin is damaged, new skin may regenerate in a normal healthy person, but the skin repair process is very difficult in some patients, such as diabetics. Furthermore, if the patient is subjected to a severe burn, affecting the endogenous stem cells, the skin cannot be repaired by itself, and superficial skin from other parts of the body must usually be transplanted to the wound, but this method causes new scarring. If the burn is large in area, the skin of the human body is almost remained, and the transplantation cannot be carried out. Without skin protection, severe burns can have severe dehydration and bacterial infections that can be life threatening, in which case artificial skin is needed.

Artificial skin is a skin substitute artificially developed in vitro by using the principles and methods of bioengineering and regenerative biology to repair and replace defective skin tissues. The artificial skin is artificially synthesized, so that the survival rate of the severe burn patient can be greatly improved. The variety of artificial skin is various, and the artificial skin which is researched and applied all over the world at present has the following three types: (1) artificial skin in the form of film or sponge made of synthetic materials such as polymer plastics, synthetic polypeptide, and artificial fiber; (2) artificial skin made of regenerated protein and animal tissue such as amnion, peritoneum, and fetal membrane; (3) sheet-like artificial skin made of fully polymerized film.

With further maturation of the technology, it has become possible to cultivate full-thickness skin having the same properties as human skin. However, most of the current artificial skins are printed manually or on a flat surface, and the production efficiency and the treatment effect are limited. Prior patent application documents disclose 3D printed skin and methods for making the same. However, in clinical treatment, in order to accelerate wound healing and promote the growth of body cells, the skin wound needs to be kept in a certain moist state, i.e. the wound is required to be neither excessively dry nor excessively moist. The artificial skin prepared by the existing method has the defects of different aspects and different degrees in the treatment process, or has poor strength due to poor adhesion; or the antigenicity is too strong, and the irritation is large; or the permeability is poor, so the fabric is easy to infect; or scar accumulation due to the inhibition of the growth of autogenous skin; or the raw materials are not easy to be obtained, so the cost is too high; or the preparation and storage are difficult due to complicated process, so that the method cannot be well popularized and clinically applied.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a with the impaired position of human skin laminate completely and have the 3D of material transport function and print skin to prior art not enough.

In order to achieve the purpose of the present invention, the present invention provides a 3D printed skin, comprising a skin layer made by 3D printing, and further comprising a plurality of microneedles, wherein at least a portion of each microneedle is embedded in the skin layer, a first end of each microneedle is penetrated to or through the surface of the skin layer, and a second end of each microneedle is close to, penetrated to or through the bottom surface of the skin layer; the microneedle comprises at least one passageway disposed between a first end and a second end.

It is from top to bottom visible, the utility model discloses a 3D prints skin and has micropin structure. After the 3D printing skin is implanted, the second end of the microneedle can be close to, contact with or pierce into the end of a receptor, the first end is attached to the outer side of the skin layer, so that nutrient solution, medicines or cells can be conveyed inwards from the outer side of the 3D printing skin, and meanwhile, the microneedle can also realize the conveying of excrement and the like from the cells in the body of an implant to the outer side of the implanted skin, so that the regeneration of the implanted skin is facilitated.

The further technical proposal is that the micro-needle is also provided with at least one through hole communicated with the passage.

From the above, the micro-needle can be provided with a plurality of through holes to realize the transportation in different modes. For example, through holes are formed on the side surfaces of the microneedles, so that the positioning input or the excrement output of the side surfaces is realized.

Further technical solutions are that the plurality of microneedles are made of the same or different materials, with the same or different orientations, lengths, cross-sections and/or microneedle spacings.

It is from top to bottom visible, the utility model discloses an adopt different micropins to carry out the overall arrangement, realize multiple transport function. Microneedle arrays formed from a plurality of microneedles can include, for example, hybrid microneedles having different lengths, outer diameters, inner diameters, cross-sectional shapes, and microneedle spacings. The utility model discloses a single micropin can have outside-in simultaneously and carry and the function of carrying from inside to outside, the utility model discloses a plurality of micropins also can have partly micropin to have the function that outside-in carried, have another part micropin to have the function of carrying from inside to outside. The orientation, length, cross-section and microneedle spacing of the microneedles may be determined based on the particular implant conditions, the nature of the substance to be delivered or delivered, etc. For example, the microneedle pitch can be adjusted according to the requirements of the skin implantation site, and the density of the microneedles can be adjusted by appropriately increasing or decreasing the number of microneedles.

The further technical proposal is that the preparation material of the micro-needle is one or more of metal, ceramic, semiconductor material, low molecular organic matter or organic high molecular material; the organic high polymer material is a biodegradable high polymer material or a non-biodegradable high polymer material. The preparation material of the microneedle is preferably a biodegradable high molecular compound.

From the top down, the microneedles of the present invention can be constructed of various materials as desired. The materials of construction may be pharmaceutical grade stainless steel or other metals, metal alloys, silicon dioxide, polymeric materials, composite materials, and the like. The polymer material may be natural polymer material or synthetic polymer. Representative biodegradable polymers include polymers of hydroxy acids such as lactic acid and glycolic acid, such as polylactide, polyglycolide, and polylactide-co-glycolide, and the like, and the biodegradable polymers can also be copolymers copolymerized with polyethylene glycol, polyanhydrides, polyorthoesters, polyurethanes, poly (butyric acid), poly (valeric acid), and poly (lactide-co-caprolactone), and the like. Representative non-biodegradable polymers include polycarbonates, polyesters, polyacrylamides, and the like. The presence of microneedles can cause pain to the patient when the nerve grows to the epidermis, and thus the microneedles should be removed or degenerated when the skin is repaired and the nerve grows back. When non-biodegradable materials are used, the microneedles should have sufficient mechanical strength so that the microneedles can remain intact when inserted into the biological barrier, when held in place for many days, and when removed. When a biodegradable material is employed, the microneedle must remain intact for a sufficient period of time to enable the microneedle to serve its intended purpose, e.g., the purpose of the microneedle being used as a catheter for delivering a drug. The micro-needle of the utility model is preferably made of biodegradable material, and the micro-needle is degraded after being implanted into the skin for regeneration, which is beneficial to maintaining the barrier between the epidermis and the connective tissue below.

The further technical proposal is that the micro-needle is vertical to the skin layer or is arranged at a certain angle relative to the skin layer.

From the above, the microneedles of the present invention can be arranged in different orientations. When the microneedles are perpendicular to the skin layer, a greater density of microneedles per unit area of skin may be provided. When the microneedles are tilted at an angle relative to the skin layer, such as when the microneedles are positioned at an angle for natural hair growth, the microneedles are advantageously better retained in place. When the microneedles adopt different orientations, the microneedles are more easily combined with the wound surface, so that excessive damage to the skin is avoided, and the skin regeneration is facilitated.

A further solution is that the length of the microneedles is between 1 μm and 5mm, preferably between 400 μm and 1mm second end, and may be about 800 μm, for example.

From top to bottom, the utility model discloses a micropin length can be selected according to actual need. The length selection may be made with consideration for both the portions of the microneedle that are inserted into the skin and not inserted into the skin for a particular application.

The further technical scheme is that the cross section of the microneedle is circular or non-circular, and the cross section has the same or different shapes and sizes at different positions in the length direction of the microneedle.

Therefore, the utility model discloses can select suitable micropin cross sectional shape and size according to actual need. For example, when it is desired to deliver a more viscous substance through a passageway, microneedles having larger cross-sectional areas may be selected and larger passageways and/or larger through-holes provided. The microneedles may be straight, having a substantially uniform diameter. The microneedles may be tapered, with the diameter of the microneedle being at a maximum at one end and tapering to a point at the other end. Microneedles can also be manufactured having non-tapered portions including straight and tapered portions. Preferably, the microneedles have a certain difference in size, e.g., diameter, at the epidermal side and at the subcutaneous end.

The further technical scheme is that the micro-needle is provided with a sensor, and the sensor is used for monitoring and/or controlling the transmission of substances in the micro-needle.

It is from top to bottom visible, the utility model discloses can be through setting up the sensor on the micropin, the effect of the material of monitoring input to control transportation process, thereby further be favorable to implanting the regeneration of skin. The utility model discloses can detect and monitor through external equipment supporting with 3D printing skin to promote the implantation effect of skin more effectively.

The first end of the micro needle is connected with a reservoir, and the reservoir is filled with medicament, nutrient solution or cells; the reservoir controls the release of the agent, nutrient solution or cells by the sensor.

From top to bottom, the utility model discloses can be through micropin input medicament, nutrient solution or cell. The agent may include minocycline to prevent infection and maintain the cells. The medicament can also comprise traditional Chinese medicine extracts and the like. The nutrient solution may include growth factors, such as factors that stimulate nerve growth, for stimulating nerve growth to the epidermis, so that the implanted skin can protect itself in response to touch, pain, stretching, pressure, etc., and additionally, can promote hair growth. The infused cells may also include mesenchymal stem cells with minocycline, and the like. The utility model discloses set up the storage apparatus, be connected through the first end of storage apparatus and micropin, medicament, nutrient solution or the cell that is convenient for store up or produce in the storage apparatus flows out from the storage apparatus, through the passageway of micropin, flows into in the target tissue. When attached to the reservoir, it is preferred that the first ends of the microneedles protrude through the surface of the skin. In addition, a sensor may be used to release the substance of the reservoir.

Because the key to successful transplantation of artificial skin is rapid vascularization, the skin should be provided with nutrition as soon as possible after transplantation. The nutrient solution can also comprise a factor (VEGF) gene which can promote vascularization, and the VEGF gene is successfully transferred into human fiber cells to make the human fiber cells secrete VEGF so as to promote vascularization. The utility model can also implant autologous endothelial cells and fibroblasts into the dermal scaffold through a proper way to induce the formation of new blood vessels. At present in clinical application, for example the skin method is planted to sieve form cuts a lot of osculums with the artificial skin who plans the skin grafting, is fixed in the surface of a wound under certain tension condition, can increase the area of skin piece, also is convenient for simultaneously the exudate drainage, and the utility model discloses a 3D of micropin structure prints skin after, need not the incision again, and the exudate drainage is realized to the accessible micropin, is favorable to improving the implantation effect, promotes to form new blood vessel.

The further technical scheme is that the 3D printing skin further comprises a support, and the support is arranged on the surface, under the bottom surface or in the skin layer. Preferably, the stent is made of a biodegradable material.

It is from top to bottom visible, the utility model discloses a 3D prints skin includes the support, and the support helps implanting skin fixed, and the operation of being convenient for is sewed up. The stent may be a continuous whole, may have a gap in the middle, or may be divided into multiple stents, as long as it can support in the skin layer. Preferably, the scaffold is made of a biodegradable and absorbable biopolymer nanofiber material. The scaffold can be degraded or removed after 3D printed skin grafting.

The further technical proposal is that the skin layer comprises a dermis cell layer and an epidermis cell layer from inside to outside. Further, collagen gel layers are respectively arranged between the dermis cell layer and the epidermis cell layer, and on the inner side of the dermis cell layer and the outer side of the epidermis cell layer. Alternatively, the skin layer is a dense membrane layer.

From top to bottom, the utility model discloses a 3D prints skin can include real leather cell layer and epidermal cell layer, is close human skin structure, can generate cuticle and hair. The cell layer may carry antibiotics such as minocycline. When this skin configuration is employed, the second ends of the microneedles reach at least the dermal layer of the 3D printed skin to satisfy delivery of the nutrients or agents. The collagen gel material may also be replaced by other biodegradable materials or mixtures with other biodegradable materials. The utility model discloses a 3D prints skin can also be fine and close rete, is mixed fused deposition 3D by polycarboxylic anhydride and poly succinic acid ethylene glycol ester and prints the shaping, fills the input through the micropin after the implantation by collagen, polylysine, glycine, arabic gum, citric acid, have antibiotic antiviral effect's medicine etc. can further reduce the manufacturing degree of difficulty of whole skin like this.

The utility model discloses a 3D prints skin accessible following preparation method and obtains, this method includes following step: scanning the damaged part of the skin by using a 3D scanner, and establishing a base model and a 3D printing skin model; printing a substrate by using a 3D printer, and then printing the skin layer and the microneedles layer by layer; or printing a substrate by using a 3D printer according to the model, printing skin layers layer by layer, and then inserting prepared microneedles; or, printing a substrate according to the model by using a 3D printer, placing prepared microneedles, and then printing a skin layer by layer.

From top to bottom, the utility model discloses a 3D scanning modeling technique and 3D layered printing, the successive layer method of piling up print out the skin tissue that has specific thickness and specific shape that is located the impaired position of human skin, can print full customization type skin according to actual need. The microneedles can be formed by printing while the biological 3D layer-by-layer skin layer is printed, or the microneedles are implanted for a second time after the 3D layer is printed. The skin layer can be printed layer by layer from inside to outside or from outside to inside when the skin layer is printed layer by layer.

The further technical scheme is that the preparation method also comprises the following steps: extracting a small amount of skin tissue, separating to obtain primary skin cells, culturing to obtain the required amount of dermal cells and epidermal cells, and printing skin layer.

It is from top to bottom visible, the utility model discloses only need draw a small amount of human skin cells, can obtain the skin cell that is used for 3D to print of capacity after cell separation, medicine stimulation, subculture, avoid further causing skin wound.

The further technical proposal is that a plurality of prepared micro-needles are sterilized; the printing of the skin layer is performed in a clean closed environment.

It is from top to bottom visible, the utility model discloses a 3D prints skin and is used for implanting human surface, and it is clean to guarantee 3D to print skin as far as possible, avoids bringing the infection. The microneedles of the present invention may be sterilized using standard methods, such as ethylene oxide sterilization or gamma radiation sterilization.

Drawings

Fig. 1 is a schematic structural diagram of a first embodiment of a 3D printed skin according to the present invention, wherein (a) is a schematic diagram of a 3D printed skin; (b) is a schematic view of a microneedle-bearing stent; (c) a partial cross-sectional enlarged view of the skin is printed for 3D.

Fig. 2 is a schematic structural diagram of a second embodiment of the 3D printed skin of the present invention, wherein (a) is a schematic diagram of the 3D printed skin; (b) is a schematic view of a microneedle-bearing stent; (c) a partial cross-sectional enlarged view of the skin is printed for 3D.

Fig. 3 is a schematic structural diagram of a third embodiment of the 3D printed skin of the present invention, wherein (a) is a schematic diagram of the 3D printed skin; (b) is a schematic view of a microneedle-bearing stent; (c) a partial cross-sectional enlarged view of the skin is printed for 3D.

Fig. 4 is a schematic structural diagram of a fourth embodiment of the 3D printed skin of the present invention, wherein (a) is a schematic diagram of the 3D printed skin; (b) is a schematic view of a microneedle-bearing stent; (c) a partial cross-sectional enlarged view of the skin is printed for 3D.

Fig. 5 is a schematic diagram of a fifth embodiment of the 3D printed skin of the present invention, wherein (a) is a schematic diagram of the 3D printed skin; (b) an exploded schematic view of a 3D printed skin.

Fig. 6 is a partial cross-sectional view of the 3D printed skin of the present invention after six grafts.

Fig. 7 is a partial cross-sectional view of a 3D printed skin embodiment of the present invention after seven grafts.

Fig. 8 is a partial cross-sectional view of the 3D printed skin embodiment of the present invention after eight grafts.

The present invention will be further explained with reference to the drawings and examples.

Detailed Description

Printed skin preparation method examples

The embodiment provides a preparation method of a 3D printed skin, which includes the following steps performed in sequence:

(1) 3D scanning and skin modeling: scanning the damaged part of the skin of a human body by using a 3D scanner to obtain a three-dimensional structure of the damaged part of the skin, calculating various attributes of the full-custom skin to be transplanted to the wound by using software, including various attributes of a base three-dimensional model and a skin structure model, and establishing a 3D printing skin model which is in accordance with the three-dimensional structure of the damaged part of the skin and comprises a bracket, so that the custom skin is completely attached to the wound; estimating and evaluating whether stem cells are needed, and if so, primarily estimating the quantity of the stem cells; meanwhile, the type of nutrient solution or medicament needing to be input for promoting skin regeneration is evaluated, the structure of the microneedle, the position and the layout of the microneedle, the sizes of the microneedle passage and the through hole and the like are evaluated.

(2) When stem cells are needed, stem cells are prepared: extracting a small amount of human skin tissue, cleaning, sterilizing, weighing, chopping to obtain primarily treated skin tissue, adding corresponding protease and collagenase, and performing tissue enzymolysis to obtain free skin cell tissue fluid; filtering to obtain cell sap, centrifuging to gather cells, and inoculating the cells to a culture dish to obtain primary skin cells; performing primary culture, namely adding a ROCK inhibitor into a part of primary cells for culture, and performing subculture for a period of time to obtain the required quantity of epidermal stem cells; and (3) taking a part of primary cells, culturing without adding a ROCK inhibitor, and subculturing for a period of time to culture the dermal stem cells with the required amount. The stem cells can be dermal stem cells and endogenous epidermal stem cells. The obtained epidermal stem cells and dermal stem cells can be stored in liquid nitrogen for printing, and when printing is needed, the cells are transferred to a raw material box of a printer.

(3) 3D layered printing: printing out Polydimethylsiloxane (PDMS) substrate through the 3D printer firstly, printing a collagen gel layer on the PDMS substrate, printing a dermis cell layer on the collagen gel layer, printing the collagen gel layer on the dermis cell layer, printing a epidermis cell layer on the collagen gel layer, and printing the collagen gel layer on the epidermis cell layer. The printing process of each layer of collagen gel is as follows: printing a layer of cross-linking agent, printing a layer of collagen gel on the cross-linking agent, and then printing the cross-linking agent to fix the layer. The printing process of the cell layer is as follows: printing a layer of cross-linking agent, printing a layer of collagen gel on the cross-linking agent, printing a layer of cell layer on the collagen gel, and then printing the cross-linking agent to fix the collagen gel layer. And the number and the thickness of the layer of the eudermal cell layer, the collagen gel layer and the epidermal cell layer are determined by modeling calculation according to actual needs. In another embodiment of the present invention, the printing can be performed from the skin surface to the subcutaneous layer. The microneedles can be formed by printing while the biological 3D layer-by-layer skin layer is printed, or the microneedles are implanted for a second time after the 3D layer is printed.

After the 3D printing skin is prepared, skin tissue culture and transplantation are carried out: and transferring the printed skin into a culture dish, culturing for 2-10 days at a gas-liquid interface of a constant temperature box, and transplanting the skin tissue to the damaged part of the skin or performing an in-vitro skin model test after the skin tissue is primarily fused.

According to the 3D skin preparation method provided by the embodiment, the skin is divided into the dermal cell layer, the epidermal cell layer and the gel layer according to modeling, the full-custom skin close to the structure of the human skin is printed in a multi-layer printing and layer-by-layer stacking mode, the full-function skin can generate the cuticle and the hair, and nutrient solution or medicament can be delivered through the micro-needle arranged on the skin in the implantation process.

Print skin embodiment

The first embodiment is as follows: as shown in fig. 1, the present embodiment provides a 3D-printed skin including an epidermal cell layer 11, a dermal cell layer 12, microneedles 13, and a scaffold 14. Wherein, the first end of the micro-needle 13 penetrates the surface of the epidermal cell layer 11, the second end penetrates the bottom surface of the dermal cell layer 12, and a passage is arranged in the middle of the micro-needle 13. The microneedles 13 include straight sections and tapered sections. The microneedles 13 are made of the same material as the holder 14. The stent 14 is located on the uppermost layer, and the stent 14 can be removed after 3D printing skin graft surgery. The 3D printed skin may also comprise a layer of collagen gel, not shown in the figures.

Example two: as shown in fig. 2, the present embodiment provides a 3D-printed skin including an epidermal cell layer 21, an endothelial cell layer 22, microneedles 23, and a scaffold 24. Wherein, the micro-needle 23 and the bracket 24 are made of different materials. The stent 24 is positioned on the topmost layer of the 3D printed skin and can be removed after the 3D printed skin graft surgery. The rest of the structure is similar to the first embodiment.

Example three: as shown in fig. 3, the present embodiment provides a 3D-printed skin including an epidermal cell layer 31, an endothelial cell layer 32, microneedles 33, and a scaffold 34. Among other things, the scaffold 34 is located in a middle layer of the 3D printed skin, which can degrade after the 3D printed skin graft surgery. The microneedles 33 and the stent 34 are made of the same or different materials. The rest of the structure is similar to the first embodiment.

Example four: as shown in fig. 4, the present embodiment provides a 3D-printed skin including an epidermal cell layer 41, an endothelial cell layer 42, microneedles 43, and a scaffold 44. Where the scaffold 44 is located at the lowest layer of 3D printed skin, it may degrade after 3D printed skin grafting surgery. The microneedles 43 and the holders 44 are made of the same or different materials. The rest of the structure is similar to the first embodiment.

Example five: as shown in fig. 5, the present embodiment provides a 3D-printed skin including an epidermal cell layer 51, a dermal cell layer 52, microneedles 53, and a scaffold 54. Wherein the microneedles 53 are tapered. The scaffolds 54 are positioned on the topmost layer of the 3D printed skin, dispersed around the microneedles 53, which may be removed after the 3D printed skin grafting procedure. The microneedles 53 are made of the same or different materials as the stent 54.

Example six: as shown in fig. 6, the present embodiment provides a 3D-printed skin including an epidermal cell layer 61, an endothelial cell layer 62, microneedles 63, and a scaffold 64. Wherein a passage 65 is arranged between the first end and the second end of the microneedle 63, the microneedle 63 is tapered, and the diameter of the microneedle 63 is gradually reduced from the first end to the second end. The microneedles 63 are also provided with through-holes 66 at the sides thereof. After the 3D printed skin is transplanted, the second end of the microneedle 63 is inserted into a patient body 67, nutrient solution, medicament or cells 68 can be input from the first end of the microneedle 63 through the passage 65, the nutrient solution, medicament or cells 68 can be positioned, permeated or conveyed to the patient from the second end of the microneedle 63 or the through hole 66, in addition, waste can be discharged out of the skin through the passage 65, and the regeneration, fusion and growth of the skin are facilitated. A scaffold 64 is disposed around the microneedles 63 and in the epidermal cell layer 61 and the dermal cell layer 62, and is degradable after 3D printing of the skin graft.

Example seven: as shown in fig. 7, the present embodiment provides a 3D-printed skin including an epidermal cell layer 71, an endothelial cell layer 72, microneedles 73, and a scaffold 74. The micro-needle 73 is provided with a passage 75 and a through hole 76, the second end of the micro-needle 73 is inserted into the body 77 of a patient, and the micro-needle 73 can deliver nutrient solution, medicament or cells 78 and also can discharge waste. A scaffold 74 is disposed apart from the microneedles 73, which are located in the epidermal cell layer 71 and the dermal cell layer 72, and is degradable after 3D printing of the skin graft. The rest of the structure is similar to the sixth embodiment.

Example eight: as shown in fig. 8, the present embodiment provides a 3D-printed skin including an epidermal cell layer 81, an endothelial cell layer 82, microneedles 83, and a scaffold 84. The micro-needle 83 is provided with a passage 85 and a through hole 86, the second end of the micro-needle 83 is inserted into a patient body 87, and the micro-needle 83 can deliver nutrient solution, medicament or cells 88 and also can discharge waste. In the embodiment, the diameter of a part of the micro-needle 83 is gradually reduced from the first end to the second end, and the diameter of a part of the micro-needle 83 is gradually reduced from the second end to the first end, so that the micro-needle density is improved, the substance is conveyed, and the micro-needle can automatically and rapidly heal with the skin of a human body.

It should be emphasized that the above-described preferred embodiment of the present invention is merely exemplary and is not intended to limit the invention, since various changes and modifications may be made by those skilled in the art, any changes, substitutions, and alterations herein without departing from the spirit and scope of the invention.

The utility model provides a 3D prints skin, can be used to various skin diseases such as psoriasis, acne, patient's skin restoration such as albinism, also can be used to patient's skin restoration such as burn, diabetes. And 3D printing skin is full customization skin, and is identical with the wound completely, has improved the treatment success rate to can obtain better cure effect. Simultaneously, the utility model discloses a micropin plays important role in the aspect of 3D prints skin input nutrient solution, cell and medicine and the waste material of discharging after implanting.

Claims (7)

1. A3D prints skin, includes the skin layer that 3D printed and made, its characterized in that:

the 3D printed skin further comprises a plurality of microneedles embedded in the skin layer, a first end of the microneedles passing to or through a surface of the skin layer, a second end of the microneedles approaching, passing to or through a bottom surface of the skin layer;

the microneedle comprises at least one passageway disposed between the first end and the second end.

2. The 3D printed skin of claim 1, wherein:

the microneedle is also provided with at least one through hole communicating with the passageway.

3. 3D printed skin according to claim 1 or 2, characterized in that:

the plurality of microneedles are made of the same or different materials, with the same or different orientations, lengths, cross-sections, and microneedle spacings;

the orientation is perpendicular to the skin layer or inclined at an angle relative to the skin layer;

said length is between 1 μm and 5 mm;

the cross section is circular or non-circular, and the cross section has the same or different shapes and sizes at different positions in the length direction of the microneedle.

4. 3D printed skin according to claim 1 or 2, characterized in that:

the micro-needle is provided with a sensor, and the sensor is used for monitoring and/or controlling the transmission of substances in the micro-needle.

5. 3D printed skin according to claim 1 or 2, characterized in that:

the first end of the microneedle is connected with a reservoir, and the reservoir contains a medicament, a nutrient solution or cells; the reservoir controls the release of the agent, nutrient solution or cells by the sensor.

6. 3D printed skin according to claim 1 or 2, characterized in that:

the 3D printed skin further comprises a scaffold disposed above a surface of the skin layer, below a bottom surface of the skin layer, or in the skin layer; the stent is made of biodegradable material or non-biodegradable material.

7. 3D printed skin according to claim 1 or 2, characterized in that:

the skin layer comprises a dermis cell layer and an epidermis cell layer; collagen gel layers are respectively arranged between the dermal cell layer and the epidermal cell layer, and on the inner side of the dermal cell layer and the outer side of the epidermal cell;

alternatively, the skin layer is a polymeric dense film layer.

Images