CN1335494A - Capillary electrophoretic filtering analyzer - Google Patents
Capillary electrophoretic filtering analyzer Download PDFInfo
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- CN1335494A CN1335494A CN 01128581 CN01128581A CN1335494A CN 1335494 A CN1335494 A CN 1335494A CN 01128581 CN01128581 CN 01128581 CN 01128581 A CN01128581 A CN 01128581A CN 1335494 A CN1335494 A CN 1335494A
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Abstract
The capillary electrophoretic filtering analyzer features that the separating capillary in the optical precision has inner electrophoretic passage with annular cross section; that the detection instrument consists of annular filtering cavity unit, concentric optical fiber as linear light source connected optically with laser source, separating transparent capillary to form an annular filtering cavity together with the axle concentric optical fiber inside it, light signal sensor outside the wall of the separating capillary, the subsequent optical coupling unit and microcomputerized signal processing and display unit. All the said component parts except the last one are set inside one dark box. The instrument is universal, high in S/N ratio and very low lower limit of detection may be used for the synchronous detection during the sample liquid separation and variation.
Description
Technical field: the present invention relates to Modern Analytical Instrument, relate in particular to the structure innovation of capillary electrophoresis analysis instrument.
Background technology: existing capillary electrophoretic analysis method, its detecting instrument has: Ultraviolet Detector, fluorescence detector, laser-induced fluorescence detector, the Electrochemical Detection instrument, chemiluminescence detector, Mass Spectrometer Method instrument etc., all these detection methods and instrument, its characteristics and scope of application restriction is separately all arranged, be that versatility is relatively poor, and common shortcoming mainly is: can only be at the Capillary Electrophoresis Separation Process point, separated sample liquid is extracted earlier, detect again, it promptly all is single-point, non-detection simultaneously, at all can't be in capillary electrophoresis separation, the separation of multiple spot even omnidistance inspection sample measuring liquid and the situation of variation, thereby a large amount of physics and chemistry information of having omitted detected sample liquid.
Summary of the invention: the objective of the invention is shortcoming, design a kind of highly versatile, and can separate, change the omnidistance capillary electrophoretic filtering analyzer that carries out synchronous detection to sample liquid at existing capillary electrophoresis analysis detector.
For achieving the above object, being constructed as follows of capillary electrophoretic filtering analyzer of the present invention: separate with kapillary two ends difference UNICOM's sample inlet pool and sample-out pool, and iontophoretic electrode and ground-electrode insert respectively into and out of the sample pond, and adjacent capillaries into and out of the sample end; Detecting instrument then detects, shows the concentration of separated sample liquid, it is characterized in that: described separation kapillary is the transparent thin tube with optical accuracy, and the electrophoresis path xsect in it is annular; Described detecting instrument is by constituting with lower unit: the toroidal cavity filter unit, by the axle center light transmitting fiber that connects as optics with monochromatic source as line source, be built in described separation with on the capillary transparent tube axle center, light transmitting fiber surface, axle center with separate with forming the optical filtering toroidal cavity between the tube wall of capillary transparent tube, and outside the tube wall of described separation with the capillary transparent tube, be provided with optical signal sensor, to pick up light signal from this tube wall; Be orderly connected with photoelectrical coupling unit, reach signal Processing/display unit,, show after then electric signal being dealt with so that light signal conversion, amplification that optical signal sensor was picked up are become electric signal thereafter.
Described optical signal sensor is some sensing light transmitting fibers, along separating with capillary transparent tube axial distribution, and does direct sunshine student's federation in appearance at its tube wall and connects and constitute; Or imaging lens, place on the image space that separates with capillary transparent tube tube wall to constitute.
Described axle center light transmitting fiber passes through described separation capillary transparent tube total length; The axle center light transmitting fiber, and the sensing light transmitting fiber can be multi-mode or monotype.
Described monochromatic source is the LASER Light Source of frequency adjustable.
Described signal Processing/display unit is a microcomputer system, and numeral is handled and be shown as to its electric signal with photoelectrical coupling unit output.
Described separation with capillary transparent tube, sample inlet pool and sample-out pool, iontophoretic electrode and ground-electrode, optical signal sensor, and photoelectrical coupling unit all place among the camera bellows.
Capillary electrophoretic filtering analyzer of the present invention, owing to will separate with the electrophoresis circular passage of capillary transparent tube and the optical filtering toroidal cavity of detecting instrument and integrate, thereby separate with the capillary transparent tube axially on any point place, between monochromatic line source surface and capillary transparent tube tube wall, reflection, the refraction that can produce monochromatic various patterns at any time; And characteristic frequency monochromatic light is in certain detected sample liquid, and other effects can be ignored, and only produces anti-, refraction, and structure, the content of various components are related deeply in anti-, refractive index and the sample liquid, produces the mode filtered light strength signal of sample liquid thus; In conjunction with factors such as electrophoretic voltage, electric current, electrophoretic velocities, be equipped with the processing/demonstration of micro computer rapid data, the present invention can be when doing the separation of sample liquid, sample liquid is carried out multiple spot even omnidistance Synchronization Analysis detect, show synchronously testing result, can detect interaction and the variation etc. of sample fluid component in detachment process, greatly enrich the physics and chemistry quantity of information of detected sample liquid; And the line source of employing optical fiber side scattering laser, light source monochromaticity is good, and background value significantly reduces, and can obtain very high signal to noise ratio (S/N ratio), and very low detection lower limit has increased resolution, sensitivity; The invention belongs to folding, the reflection quasi-instrument of light, have very strong versatility, separated sample liquid is not carried out any processing, saved time, reagent, simplified experimental procedure.Therefore, the present invention is the analysis and detection technology that does not still reach in the world up to now.
Description of drawings: now in conjunction with the accompanying drawings the present invention is made the following instructions.
Fig. 1 is the structural principle synoptic diagram of the embodiment of the invention.
Fig. 2 is the structural representation of the embodiment of the invention 1.
Fig. 3 is the structural representation of the embodiment of the invention 2.
Fig. 4 is electrophoresis of the present invention circular passage broken section and mode filtered light synoptic diagram.
The specific embodiment: as shown in Figure 1, the formation of capillary electrophoretic filtering analyzer of the present invention As follows: as separate with capillary 1 two ends difference UNICOM sample inlet pool 2 and sample-out pool 3, and iontophoretic electrode 4 to reach Earth electrode 5 inserts respectively into and out of the sample pond, and adjacent capillaries into and out of the sample end; Detecting instrument Then the concentration to separated sample liquid detects, shows, it is characterized in that: described separation capillary Pipe 1 is the transparent thin tube with optical accuracy, and electrophoresis path 8 cross sections in it are annular; Described detecting instrument is by consisting of so that lower unit is following: the toroidal cavity filter unit, by with monochromatic source 6 Make the axle center optical fiber 7 of optics connection as line source, it is transparent with capillary to be built in described separation Manage on 1 axle center, axle center optical fiber 7 surfaces with separate with forming filter between the tube wall of capillary penetrating exposed conduit 1 Ring of light shape cavity 8 outside the tube wall of described separation with capillary penetrating exposed conduit 1, is provided with optical signal sensor 9, To pick up optical signal from this tube wall; Be connected with photoelectrical coupling unit 10, with the light letter that picks up thereafter Number conversion, amplify and to become the signal of telecommunication; And signal processing/display unit 11, then with photoelectrical coupling unit 10 The signal of telecommunication of output is processed and is shown. Above-mentioned separation with capillary penetrating exposed conduit 1, sample inlet pool 2 with go out Sample pond 3, iontophoretic electrode 4 and earth electrode 5, optical signal sensor 9, and photoelectrical coupling unit 10 equal Place among the camera bellows 12.
As shown in Figure 2, being constructed as follows of capillary electrophoretic filtering analyzer embodiment 1 of the present invention: Separate with capillary 1 two ends difference UNICOM sample inlet pool 2 and sample-out pool 3, and iontophoretic electrode 4 and ground connection electricity The utmost point 5 inserts respectively into and out of the sample pond, and adjacent capillaries into and out of the sample end; Detecting instrument is then to quilt Quality or the concentration of separation sample liquid detect, show, it is characterized in that: described separation capillary Pipe 1 is the transparent thin tube with optical accuracy, and electrophoresis path 8 cross sections in it are annular; Described detecting instrument is by consisting of so that lower unit is following: the toroidal cavity filter unit, and by adjustable with frequency LASER Light Source 6-1 make axle center optical fiber 7 that optics connects as line source, be built in described branch From with on capillary penetrating exposed conduit 1 axle center and pass through its total length, axle center optical fiber 7 surfaces with separate usefulness Form optical filtering toroidal cavity 8 between the tube wall of capillary penetrating exposed conduit 1, set optical signal sensor is some biographies Fiber 9-1 is led in sensitization, uses capillary penetrating exposed conduit 1 axial distribution along separating, and does in appearance directly at its tube wall Connect optics and connect and consist of, to pick up optical signal from this tube wall; Be connected with photoelectrical coupling unit thereafter 10, optical signal conversion, the amplification of picking up become the signal of telecommunication; And the microcomputer type signal is processed/show Unit 11-1 then processes and is shown as numeral with the signal of telecommunication of photoelectrical coupling unit 10 outputs. On The separation of stating capillary penetrating exposed conduit 1, sample inlet pool 2 and sample-out pool 3, iontophoretic electrode 4 and earth electrode 5, Sensing optical fiber 9-1, and photoelectrical coupling unit 10 all place among the camera bellows 12.
As shown in Figure 3, being constructed as follows of capillary electrophoretic filtering analyzer embodiment 2 of the present invention: Separate with capillary 1 two ends difference UNICOM sample inlet pool 2 and sample-out pool 3, and iontophoretic electrode 4 and ground connection electricity The utmost point 5 inserts respectively into and out of the sample pond, and adjacent capillaries into and out of the sample end; Detecting instrument is then to quilt Quality or the concentration of separation sample liquid detect, show, it is characterized in that: described separation capillary Pipe 1 is the transparent thin tube with optical accuracy, and electrophoresis path 8 cross sections in it are annular; Described detecting instrument is by consisting of so that lower unit is following: the toroidal cavity filter unit, and by adjustable with frequency LASER Light Source 6-1 make axle center optical fiber 7 that optics connects as line source, be built in described branch From with on capillary penetrating exposed conduit 1 axle center and pass through its total length, axle center optical fiber 7 surfaces with separate usefulness Form optical filtering toroidal cavity 8 between the tube wall of capillary penetrating exposed conduit 1, set optical signal sensor is imaging lens 9-2 places on the image space of separation with the tube wall of capillary penetrating exposed conduit 1 to consist of, to pick up from this tube wall Get optical signal; Be connected with photoelectrical coupling unit 10, the optical signal that picks up is changed, zoomed into thereafter Be the signal of telecommunication; Microcomputer type signal processing/display unit 11-1 is then with photoelectrical coupling unit 10 outputs The signal of telecommunication process and be shown as numeral. Above-mentioned separation with capillary penetrating exposed conduit 1, sample inlet pool 2 with Sample-out pool 3, iontophoretic electrode 4 and earth electrode 5, imaging lens 9-2, and photoelectrical coupling unit 10 equal Place among the camera bellows 12.
As shown in Figure 4, the axle center optical fiber 7 of connecting monochromatic source places on capillary penetrating exposed conduit 1 axle center that can accurately filter, form optical filtering toroidal cavity 8 between the tube wall of the surface of axle center optical fiber 7 and capillary penetrating exposed conduit 1, refraction, reflection in succession can take place in the inside and outside surface of the tube wall of axle center optical fiber 7 surfaces, capillary penetrating exposed conduit 1 in monochromatic light, certain root sensing optical fiber 9-1 (or imaging lens 9-2, see Fig. 3) light intensity that can collect is exactly certain initial homogeneous beam I0Process is such as pattern In, pattern In+1Deng in succession refraction, reflection approach (pattern) arrive this sensing optical fiber 9-1 (or imaging lens 9-2, See Fig. 3) all light summations, i.e. mode filtered light signal. When detected sample liquid electrophoresis filters annular in this When cavity 8 is interior, can adopt CF monochromatic light, make other effect quilts between detected sample liquid and light Ignore, then the mode filtered light collected of sensing optical fiber 9-1 (or imaging lens 9-2, see Fig. 3) The variation of signal strength signal intensity is the mode filtered light signal of this detected sample liquid. Pattern with this detected sample liquid Optical filtering signals is processed/is shown in conjunction with factor integrations such as electrophoretic voltage, electric current, electrophoretic velocities, namely realizes Do to detect synchronously to analyze when sample liquid do is separated.
Now mainly in conjunction with the embodiment of the invention 1 and above-mentioned accompanying drawing, further specify operation principle and process As follows: at first open the power supply of iontophoretic electrode 4, LASER Light Source 6-1 and all unit of detecting instrument, right Separate and detect with the homogeneous carrier fluid that is full of in the capillary penetrating exposed conduit 1 electrophoresis circular passage 8, get clearancen White control value; To treat that then sample measuring liquid is as containing mixing of aminoacetic acid 9.8mMol and alanine 10.0mMol Sample liquid injects the sample inlet pool 2 be filled with carrier fluid since into and out of sample pond 2,3 and separate and use capillary penetrating Be full of the carrier fluid of homogeneous in the electrophoresis circular passage 8 of exposed conduit 1,5 of iontophoretic electrode 4 and earth electrodes Set up the electrophoresis electric field, aggregate sample liquid is under the effect of this electrophoresis electric field force, through separating with hair The electrophoresis circular passage 8 of thin transparent pipe 1,3 directions move as electrophoresis from sample inlet pool 2 to sample-out pool, and In moving process, separate, thereby at the different moment, capillary penetrating exposed conduit 1 axial different sections On the optical signal sensing optical fiber 9-1 on the tube wall face, can collect detected sample liquid different phase, difference The mode filtered light signal of intensity is sent into microcomputer type signal processing/display unit 11-1 again and is shown as difference Peak value: at the beginning, the root optical signal sensing optical fiber 9-1 of nearly sample introduction end place of capillary penetrating exposed conduit 1 On, microcomputer type signal processing/display unit 11-1 shows unique peak that mixes of its aminoacetic acid and alanine Value is along with passage of time, at the sample introduction end far away place of capillary penetrating exposed conduit 1 root optical signal sensing photoconduction fibre On the dimension 9-1, microcomputer type signal processing/display unit 11-1 shows the separation peak value that two is different, Time continues, and is separating with the optical signal sensing optical fiber behind certain axial location of capillary penetrating exposed conduit 1 On the 9-1, the tolerance of the separation peak value that demonstrates all reaches maximum and is tending towards the constant balance of namely separating, this The time peak value that separates tolerance be directly proportional with the molar concentration of aminoacetic acid and alanine respectively, namely realized The synchronous detection analysis that sample liquid separates; In any moment, microcomputer type signal processing/display unit 11-1 can To do separating with the optical signal on capillary penetrating exposed conduit 1 axially upper all optical signal sensing optical fiber 9-1 Simultaneously scanning separates omnidistance synchronous detection analysis to realize sample liquid.
Claims (6)
1, a kind of capillary electrophoretic filtering analyzer, it is constructed as follows: separate with kapillary two ends UNICOM's sample inlet pool and sample-out pool respectively, and iontophoretic electrode and ground-electrode insert respectively into and out of the sample pond, and adjacent capillaries into and out of the sample end; Detecting instrument then detects, shows the concentration of separated sample liquid, it is characterized in that: described separation kapillary is the transparent thin tube with optical accuracy, and the electrophoresis path xsect in it is annular; Described detecting instrument is by constituting with lower unit: the toroidal cavity filter unit, by the axle center light transmitting fiber that connects as optics with monochromatic source as line source, be built in described separation with on the capillary transparent tube axle center, light transmitting fiber surface, axle center with separate with forming the optical filtering toroidal cavity between the tube wall of capillary transparent tube, and outside the tube wall of described separation with the capillary transparent tube, be provided with optical signal sensor, to pick up light signal from this tube wall; Be orderly connected with photoelectrical coupling unit, reach signal Processing/display unit,, show after then electric signal being dealt with so that light signal conversion, amplification that optical signal sensor was picked up are become electric signal thereafter.
2, capillary electrophoretic filtering analyzer as claimed in claim 1, it is characterized in that: described optical signal sensor, be some sensing light transmitting fibers, along separating, and do direct sunshine student's federation in appearance at its tube wall and connect and constitute with capillary transparent tube axial distribution; Or imaging lens, place on the image space that separates with capillary transparent tube tube wall to constitute.
3, capillary electrophoretic filtering analyzer as claimed in claim 1 or 2 is characterized in that: described axle center light transmitting fiber passes through described separation capillary transparent tube total length; The axle center light transmitting fiber, and the sensing light transmitting fiber can be multi-mode or monotype.
4, capillary electrophoretic filtering analyzer as claimed in claim 1 or 2 is characterized in that: described monochromatic source is the LASER Light Source of frequency adjustable.
5, capillary electrophoretic filtering analyzer as claimed in claim 1 or 2 is characterized in that: described signal Processing/display unit is a microcomputer system, and numeral is handled and be shown as to its electric signal with photoelectrical coupling unit output.
6, capillary electrophoretic filtering analyzer as claimed in claim 1 or 2 is characterized in that: described separation with capillary transparent tube, sample inlet pool and sample-out pool, iontophoretic electrode and ground-electrode, optical signal sensor, and photoelectrical coupling unit all place among the camera bellows.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011285818A CN1156693C (en) | 2001-09-06 | 2001-09-06 | Capillary electrophoretic filtering analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011285818A CN1156693C (en) | 2001-09-06 | 2001-09-06 | Capillary electrophoretic filtering analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1335494A true CN1335494A (en) | 2002-02-13 |
| CN1156693C CN1156693C (en) | 2004-07-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011285818A Expired - Fee Related CN1156693C (en) | 2001-09-06 | 2001-09-06 | Capillary electrophoretic filtering analyzer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394172C (en) * | 2005-01-25 | 2008-06-11 | 中国科学院化学研究所 | Micro-capillary electropheresis apparatus |
| CN101334378B (en) * | 2008-07-29 | 2012-05-16 | 清华大学 | Portable capillary pipe electrophoresis apparatus |
-
2001
- 2001-09-06 CN CNB011285818A patent/CN1156693C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100394172C (en) * | 2005-01-25 | 2008-06-11 | 中国科学院化学研究所 | Micro-capillary electropheresis apparatus |
| CN101334378B (en) * | 2008-07-29 | 2012-05-16 | 清华大学 | Portable capillary pipe electrophoresis apparatus |
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| Publication number | Publication date |
|---|---|
| CN1156693C (en) | 2004-07-07 |
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