CN1331978A - Application of sowthistle in preparing tobacco toxin resisting medicine - Google Patents
Application of sowthistle in preparing tobacco toxin resisting medicine Download PDFInfo
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Abstract
The ethanol or water extract of sowthistle plant has the function of resisting tobacco toxin and may be prepared to tobacco toxin resisting medicine. The medicine, after being taken, can resist the effect of harmful matter in cigarette and reduce the damage of cigarette to smoker and other people.
Description
The present invention relates to the application of sonchus plant, the particularly application of sonchus plant in the anti-tobacco poisoning medicine of preparation.
Contain carbohydrate, protein, organic acid, alkaloid, glycoside, resin and the multiple composition of volatilization wet goods in the Nicotiana tabacum L..Complicated chemical change takes place in these materials in the Nicotiana tabacum L. of lighting, can produce thousands of kinds of chemical substances.According to surveying and determination, the tar that discharges in the Linear Leaf Speedwell cigarette combustion process is up to 40mg, nicotine 2.6mg, carbon monoxide 100mg, carbon dioxide 150mg and ammonia, Blausure (German), various organic volatiles, phenols and polycyclic aromatic hydrocarbon also have complicated ingredients such as heavy metal such as cadmium, arsenic, lead and radioactive substance.Wherein a lot of harmful substances, tens kinds of carcinogenic and short cancer materials.The Japan scientist finds also to contain in the Nicotiana tabacum L. the strong carcinogenic noxious substance of dioxin in the recent period.
A large amount of studies show that smoking serious harm health.Smoking is more fast-growth 50% than non smoker arterial sclerosis, inspires cardiovascular and cerebrovascular disease.Smoking can be brought out multiple cancer, especially hepatocarcinoma and pulmonary carcinoma is had a direct impact, and impels respiratory tract disease, asthma and bronchitic generation and development.Our result of study has illustrated that also smoking all has major injury to liver, lung and kidney.Smoking can also endanger people's vision, audition and nervous system.Owing to the nuisance mass-energy in the cigarette is quickened body generation free radical, and reduce superoxide dismutase (SOD) activity, thereby quicken body aging and the generation of bringing out various diseases.The harmful substance contents that is dispersed in the smog on every side manys 1-4 doubly than the intravital harmful substance contents of the direct suction of smoker people, so passive smoking faces serious health problem equally.
World Health Organization (WHO) statistics, 3,000,000 people are arranged every year in the world because the disease that smoking causes and death.If continue by this number of smokers and amount of smoking, after 20 years, because of the number of smoking death may be increased to annually 1000 ten thousand, the overwhelming majority belongs to developing country.
Agriculture service bureau of foreign country of United States Department of Agriculture (USDA) announces that World tobacco output in 1998 is up to more than 700 ten thousand tons, and the tobacco production of China ranks first in the world, and accounts for Gross World Product more than 1/3.Smoker's sum in the world has 1,000,000,000 crowd, and the smoker of China accounts for more than 300,000,000.There is trend ahead of time in the age ratio past that investigation Chinese male surname of demonstration and women surname begin smoking.Everyone average cigarette consumption amount of China has leapt to the hat in the world.Because the smoking healthhazard is familiar with by increasing people, the whole world national governments and the people, particularly developed country takes action, and carries out anti-smoking campaign.The large-scale tobacco company of states such as the U.S. more and more is difficult to market sale home, and they are the sale of medicated cigarette aiming market, Asia, particularly developing countries such as China, Vietnam.Countries such as the leading exporter U.S. of Medicated cigarette, Holland, Britain, Brazil, Germany.
In order to help the smoker to give up smoking, people have studied various smoking abstention methods and smoking cessation product.Present popular method of giving up smoking has nicotine replacement method such as smoking give-up chewing gum; Be attached to plaster on the skin and nasal spray etc.Also have acupuncture therapy and Zyban smoking deterent etc.But these smoking cessation ways all can not make most ESs adhere to giving up smoking more than 1 year effectively; Some medicine also has side effect, such as the Zyban smoking deterent common pair of effect of xerostomia, insomnia is arranged, and other drug has frees eczema, side effect such as anaphylaxis pruritus, urticaria, have in addition can cause breaking out of some disease.
People attempt to reduce the harm to the smoker with the content that reduces tar in the cigarette, have produced various low tar medicated cigarettes.But relevant the survey showed that, and it can mislead the smoker, drawn up this cigarette, too have a hazardness.
The objective of the invention is to overcome many defectives of above-mentioned smoking abstention method and stop smoking medicine existence, the application of a kind of sonchus plant in the anti-tobacco poisoning preparation of preparation is provided.The extract of the organic solvent of sonchus Sonchus plant or water has been carried out the experimentation of anti-tobacco poisoning, found that this platymiscium has good anti-tobacco poisoning effect.Promptly utilize the sonchus plant to prepare anti-tobacco poisoning preparation, after the smoker takes this anti-tobacco poisoning preparation, can facedown suck the effect of intravital cigarette harmful matter, reduce in the Nicotiana tabacum L. harmful substance effectively smoker and involuntary smoker's harm.
Embodiment of the present invention are as follows:
The present invention is the application of sonchus plant in the anti-tobacco poisoning medicine of preparation;
Make raw material with the sonchus plant, the processing step for preparing anti-tobacco poisoning medicine is:
1. after herbaceos perennial Herba Sonchi Arvensis Sonehus arvensis L. being cleaned up, air-dry, pulverizing;
2. the Herba Sonchi Arvensis after will pulverizing carries out heating and refluxing extraction with ethanol or water, filter extracting solution; The heating and refluxing extraction temperature is 60 ℃-100 ℃;
3. the extracting solution after will filtering makes anti-tobacco poisoning extractum through concentrating under reduced pressure;
4. the anti-tobacco poisoning extractum that will make is being lower than under 100 ℃ the temperature, and is dry and pulverize and make anti-tobacco poisoning medicated powder, incapsulate, and the anti-tobacco poisoning medicated powder of the 0.25g-0.3g that packs in each capsule, thus make anti-tobacco poisoning capsule;
Perhaps will resist tobacco poisoning extractum to add starch, magnesium stearate and Pulvis Talci are made anti-tobacco poisoning tablet through tabletting, and every tablet of anti-tobacco poisoning tablet contains the anti-tobacco poisoning extractum of 0.1-0.4g, and the outside can be surrounded by sugar-coat;
Also can be made into anti-tobacco poisoning medicine grain or anti-tobacco poisoning pill, anti-tobacco poisoning pill can be honeyed pill.
The application of sonchus plant provided by the invention in the anti-tobacco poisoning preparation of preparation at home and abroad do not appear in the newspapers so far as yet.This anti-tobacco poisoning preparation does not have toxic and side effects, from the plant of integration of edible and medicinal herbs, after the smoker takes this anti-tobacco poisoning preparation, can facedown sucks the effect of intravital cigarette harmful matter, reduces in the Nicotiana tabacum L. harmful substance effectively to smoker and involuntary smoker's harm.
Further describe the anti-tobacco poisoning effect of sonchus Sonchus plant extract below in conjunction with experiment:
We have carried out anti-tobacco poisoning experiment to the ethanol extraction of following five kind of plant in the sonchus, its experimental technique is: after five kind of plant of sonchus are cleaned up respectively, air-dry, pulverizing, with ethanol heating and refluxing extraction in water-bath, the heating and refluxing extraction temperature is 80 ℃, and extracting solution boils off solvent through concentrating under reduced pressure and gets extract.Described five kind of plant are that Herba Sonchi Arvensis Sonchus arvensis L. is Son chus arvensis L. again, floral leaf Picris divaricata Sonchus asper L. be Da Ye lettuce Mai grass again, interrupted chrysanthemum, Sonchus brachyotus Sonchus brachyotus DC., Herba Sonchi Oleracei Sonchus oleraceusL. and full leaf Herba Sonchi Oleracei Sonchus transcaspicus Nevski.
Make the experiment object with the Kunming white mice, white mice body weight 20g, male and female half and half are divided into each group at random.Administering mode is gastric infusion.
The experimental result division is as follows:
1. Herba Sonchi Arvensis Sonchus arvensis L. is Son chus arvensis L. again, Compositae sonchus herbaceos perennial, and Chang Zuowei wild herbs among the people are edible.All herbal medicine, has detumescence and apocenosis at bitter cold, and effects such as blood stasis removing detoxifcation can be used for treating enteritis, dysentery, furuncle etc. and acute lymphoblastic type leukemia is had inhibitory action.The ethanol extraction of Herba Sonchi Arvensis is called for short S1.Through experiment, find that it has very strong anti-tobacco poisoning effect.
Experimental result:
1) half lethal dose of nicotine and tar is measured according to a conventional method, nicotine LD
5054.42mg/kg, tar LD
50529.2mg/kg.
2) S
1Effect to the mouse death rate of the nicotine of irritating the stomach half lethal dose respectively and tar sees Table 1.Mouse death rate reduces to 20% by 50%.
Table 1 S
1Influence to mouse death rate
| Group | Example number (n) | Dosage mg/kg | Time (h) | Mortality rate (%) |
| The nicotine group | ???10 | ??????54.4 | ???24 | ????50 |
| ?S 1+ nicotine group | ???10 | ??3750＋54.4 | ???24 | ????20 |
| The tar group | ???10 | ?????529.2 | ???24 | ????50 |
| ?S 1+ tar group | ???10 | ??3720＋529.2 | ???24 | ????20 |
3) S
1Pathological changes to nicotine and each internal organs of tar induced mice all has significant protective effect.
The nicotine model group: use the nicotine dosage 1.09mg/kg.d of 2% half lethal dose, after 14 days, Mild edema appears in 80% animal liver tissue, the crowded and steatosis of the hepatic cords that has.10% animal kidney has blood stasis and lung large stretch of consolidation to occur.S
1Medication group dosage 7550mg/kg.d adds nicotine 1.09mg/kg.d, and after 14 days, the liver of all animals, lung, kidney, the heart, harmonization of the stomach splenic organs show no obvious abnormalities.
The tar model group: use the tar dosage 10.58mg/kg.d of 2% half lethal dose, after 14 days, 90% animal liver Mild edema occurs or hepatic tissue has the black particle calmness.Tool taper coagulation necrosis focus and large stretch of consolidation appears in pulmonary and protein oozes out phenomenon under the 10% animal nephridial tissue tunicle.S
1Administration group agent 7500mg/kg.d adds tar 10.58mg/kg.d, and after 14 days, the liver of all animals, lung, kidney, the heart, each internal organs of harmonization of the stomach spleen show no obvious abnormalities.
4) S
1Influence to mice hemoglobin (HB), fat value peroxide (LPO value) and superoxide dismutase (SOD):
Use the nicotine (0.54mg/kg.d) and the tar (5.29mg/kg.d) of 1% half lethal dose to irritate the stomach mice respectively 14 days.Cause HB and significantly rising and the active remarkable decline of SOD of LPO value in the mice hemoglobin, and the S of administration simultaneously
1(7500mg/kg.d), show its significant anti-tobacco poisoning effect, experimental result sees Table 2:
The active variation of HB, LPO and SOD in table 2 mice plasma
| Group | Example number (n) | Time (d) | ??????HB ?????(g/L) | ???????LPO ????(μmol/L) | SOD activity (u/g) |
| Normal group | ???10 | ???14 | ??128.7±5.4 | ???0.6478±0.2 | ???504.5±46.2 |
| The nicotine group | ???10 | ???14 | ??151.5±8.9 | ???2.814±1.37 | ???418.4±7.8 |
| ?S 1+ nicotine group | ???10 | ???14 | ??136.6±3.0 | ???0.4823±0.0 | ???571.5±48.7 |
| The tar group | ???10 | ???14 | ??145.3±9.3 | ???1.1566±0.6 | ???209.6±17.7 |
| ?S 1+ tar group | ???10 | ???14 | ??123.3±9.3 | ???0.3671±0.1 | ???462.6±16.2 |
Annotate: compare P＜0.01 with normal group, compare P＜0.01 with the simulation group
Owing to nicotine and tar all can cause the animal blood slurry anoxia hemoglobin (HB) is raise.And quicken body and in metabolism, produce free radical, the lipid peroxide of free-radical generating causes the change of cellular metabolism and function again, impels body aging and brings out the generation and the development of multiple diseases such as cancer.And superoxide dismutase (SOD) can be removed free radical, suppresses the generation of body endoperoxide, and slow down aging wards off disease.
Every detection data from table 2 can be found out, two groups of administration S
1Group only 14 days, HB drops to approaching and is lower than the normal group level.Two groups LPO value all is lower than normal group.And two groups SOD activity is higher than and near normal group.
5) S1 is to the protective effect of rat passive smoking:
Rat is divided into each group, 10 every group, male and female half and half.Be placed in the special range hood, with Qincheng city board medicated cigarette, wherein nicotine content 1.4mg/ props up, and tar 23mg/ props up, and carries out the smoking experiment.Every day 6 times, each 3, totally 30 days, stop smoking.One group of smoking model group rat extracting blood is carried out leukocyte WBC, Hb H B, glutamic oxaloacetic transaminase, GOT AST (original name GOT) and the every mensuration of glutamate pyruvate transaminase ALT (original name GPT), and animal liver, heart arteria coronaria, lung and bronchus internal organs are carried out the pathological anatomy inspection, compare with matched group (non-suction smoking group).Every biochemistry detection the results are shown in Table 3 in the blood:
Table 3 passive smoking is to the influence of WBC, HB, ASL and ALT
| Group | ??WBC(10 9/L) | ??????HB(g/L) | ????AST(u) | ?????ALT(u) |
| Matched group smoking group | ???7.7±0.9 ???7.9±0.8 | ????145.4±6.4 ????198.4±25.9 | ??55.6±6.9 ??136.5±13.3 | ???51.0±4.6 ???136.0±14.8 |
Annotate: p＜0.001
Pathological examination results: the liver of blank group all animals, heart arteria coronaria, lung and bronchus internal organs show no obvious abnormalities.Smoking group all animals slight hepatic cell edema, the spotty necrosis that as seen hepatic tissue is dispersed in or the point-like lymphocytic infiltration is arranged and little blood vessel peripheral lymphoid cellular infiltration, 20% animal lung has lamellar hyperemia, and heart arteria coronaria and bronchus show no obvious abnormalities.
Experimental result shows: animal smoking compared with non-smoking group after 30 days, and smoking has all caused major injury to animal liver tissue and lung.30 days the smoking of analysis result explanation of blood is less to the WBC influence, and smoking front and back statistical test difference is remarkable (p＞0.05) not, and to HB, AST, ALT influence significantly.Owing to smoking causes the anoxia Hb H B that raise.And the rising of AST (GOT) and ALT (GPT) explanation liver function is undesired.
Two groups of smoking groups in addition, one group of administration S1 (dosage 2625mg/kgd) wherein, another group is given the normal saline with volume, again each treated animal is got every biochemistry detection that blood carries out above-mentioned identical content after 30 days, and each internal organs of animal are carried out the pathological anatomy inspection, with compare before and after the smoking, every biochemistry detection the results are shown in Table 4 and table 5 in the blood:
Table 4 normal saline is to the influence of WBC, HB, AST and ALT
| ???WBC(10 9/L) | ?????HB(g/L) | ??????AST(u) | ??????ALT(u) | |
| After obeying normal saline after the smoking before the smoking | ????8.7±1.3 ????9.1±1.8 ????8.9±1.3 | ???143.1±10.7 ???197.6±25.9 ???193.3±17.2 | ???55.7±7.6 ???135.0±21.3 ???134.5±22.6 | ???51.1±6.0 ???138.4±14.7 ???133.8±14.6 |
Annotate: p＜0.001 (after the smoking with smoking before ratio, after the administration with smoking after than)
Pathological anatomy result: normal saline group all animals slight hepatic cell edema, the spotty necrosis that as seen hepatic tissue is dispersed in or the point-like lymphocytic infiltration is arranged, little blood vessel peripheral lymphoid cellular infiltration.10% animal lung has lamellar hyperemia.Heart arteria coronaria and bronchus show no obvious abnormalities.
This experimental result shows: the animal liver that the clothes normal saline caused smoking after month and the damage of lung and liver function undesired do not have any improvement effect basically.
Table 5 S1 is to the influence of WBC, HB, AST and AT
| ?WBC(10 9/L) | ????HB(g/L) | ????AST(u) | ??????ALT(u) | |
| After giving SA after the smoking before the smoking | ??7.7±1.3 ??8.4±1.3 ??8.1±0.8 | ??148.2±6.2 ??208.8±9.2 ??155.8±12.5 | ???54.8±4.7 ???137.6±8.4 ???67.6±9.2 | ?????49.5±2.6 ?????136.6±29.0 ?????54.0±5.2 |
Annotate: p＜0.001 (after the smoking with smoking before ratio, after the administration with smoking after than)
The pathological anatomy result; Except 10% animal liver tissue has a small amount of lymphocytic infiltration, the liver of all the other animals, lung, heart arteria coronaria and bronchus internal organs show no obvious abnormalities.
Every biochemistry detection index is all near the level before the smoking in this experimental result and the blood, show S1 to animal because anoxia in the blood that smoking causes, the infringement of liver and lung and liver function undesired all had very strong restoration and protection effect.Its minimum effective dose is 1000mg/kg.
2. floral leaf Picris divaricata Sonchus asper L is Da Ye lettuce Mai grass again, interrupted chrysanthemum.The annual herb plant.All herbal medicine has reducing swelling and alleviating pain, and the blood stasis removing Detoxication is once as the product of using with of Chinese medicine Herba Patriniae.Floral leaf Picris divaricata ethanol extraction is called for short S
2, existing through experimentation, find that it has very strong anti-tobacco poisoning effect.
Experimental result:
1)
SThe influence that the 2 pairs of nicotine and tar cause chmice acute to poison: mouse death rate reduces to 50% and 40% by 80% respectively.Experimental result sees Table 6:
Table 6 S
2Influence to mouse death rate
| Group | Example number (n) | Dosage mg/kg | Time (h) | Mortality rate % |
| Nicotine group S 2+ nicotine group tar group S 2+ tar group | ?????10 ?????10 ?????10 ?????10 | ?????122.9 ??7352＋122.9 ?????912 ???7352＋912 | ?????24 ?????24 ?????24 ?????24 | ??????80 ??????50 ??????80 ??????40 |
2) S
2Protective effect to nicotine and each organ disease of tar induced mice
The nicotine model group: nicotine dosage 1.09mg/kg.d, after 14 days, Mild edema appears in 80% animal liver tissue, the crowded and steatosis of the hepatic cords that has.10% animal kidney has blood stasis and lung large stretch of consolidation to occur.S
2Medication group dosage 7352mg/kg.d adds nicotine 1.09mg/kg.d, and after 14 days, the liver of all animals, lung, kidney, the heart, harmonization of the stomach splenic organs show no obvious abnormalities.
The tar model group: tar dosage 10.58mg/kg.d, after 14 days, 90% animal liver Mild edema occurs or hepatic tissue has the black particle calmness.Tool taper coagulation necrosis focus and large stretch of consolidation appears in pulmonary and protein oozes out phenomenon under the 10% animal nephridial tissue tunicle.S
2Administration group dosage 7352mg/kg.d adds tar 10.58mg/kg.d, and after 14 days, the liver of all animals, lung, kidney, the heart, each internal organs of harmonization of the stomach spleen show no obvious abnormalities.
Experimental result shows S
2Cause the pathological changes of Mouse Liver, kidney and lungs device that very strong protective effect is all arranged to nicotine and tar.
3) S
2(dosage 7352mg/kg.d) is to nicotine (dosage 0.54mg/kg.d) and tar (dosage 5.29mg/kg.d), after 14 days, hemoglobin in the rising mice plasma (HGB) and lipid peroxide value (LPO) have significant reduction effect (be lower than or near normal value), and superoxide dismutase (SOD) activity of its reduction is had tangible rising effect, experimental result sees Table 7:
HGB in table 7 mice plasma, the active variation of LPO and SOD
| Group | Time (d) | ?????HGB?g/L | ????LPOμmol/L | The active u/g of SOD |
| Normal group nicotine group S 2+ nicotine group tar group S 2+ tar group | ?????14 ?????14 ?????14 ?????14 ?????14 | ???122.7±5.4 ???151.5±8.9 ???136.4±3.0 ???145.3±9.3 ???123.8±10.8 | ???0.6478±0.2 ???2.814±1.37 ???0.5606±0.113 ???1.1566±0.6 ???0.5201±0.1 | ??504.5±46.2 ??418.4±78 ??484.3±12.3 ??209.6±17.7 ??472.5±20.8 |
3. Sonchus brachyotus Sonchus brachyotus DC., herbaceos perennial.Edible.All herbal medicine has heat-clearing and toxic substances removing, treats acute pharyngitis and bacillary dysentery effect, and the leukaemia is had obvious inhibitory action.The Sonchus brachyotus ethanol extraction is called for short S
3, experimental result:
1) S
3The influence that causes chmice acute to poison to nicotine and tar.Mouse death rate is reduced to 10% and 0% by 50% respectively, and experimental result sees Table 8
Table 8 S
3Influence to mouse death rate
2.) S
3Protective effect nicotine model group to nicotine and each organ disease of tar induced mice: nicotine dosage 1.09mg/kg.d is after 14 days, and Mild edema appears in 80% animal liver tissue, the crowded and steatosis of the hepatic cords that has.10% animal kidney has blood stasis and lung large stretch of consolidation to occur.S
3Pharmaceutical composition amount 5882mg/kg.d added nicotine 1.09mg/kg.d after 14 days, and each internal organs such as the liver of 20% animal, lung, kidney show no obvious abnormalities.80% animal is the slight hepatic cell edema only, and all the other each internal organs show no obvious abnormalities.The tar model group, tar dosage 10.58mg/kg.d is after 14 days, and 90% animal liver Mild edema occurs or hepatic tissue has the black particle calmness.See that large stretch of consolidation appears in taper coagulation necrosis focus and pulmonary and protein oozes out phenomenon under the 10% animal nephridial tissue tunicle.S
3Medicine group 5882mg/kg.d and tar 1.09mg/kg.d are after 14 days, and each internal organs such as 20% animal liver, lung, kidney there is no unusually.Only outside the slight hepatic cell edema, all the other each internal organs there is no unusually 80% animal.Experimental result shows S
3Cause the pathological changes of each internal organs of mice that good protective effect is all arranged to nicotine and tar.
| Group | Example number (n) | Dosage mg/kg | Time (h) | Mortality rate % |
| Nicotine group S 3+ nicotine group tar group S 3+ tar group | ??????10 ??????10 ??????10 ??????10 | ?????54.4 ??5880＋54.4 ????529.2 ??5880＋529.2 | ?????24 ?????24 ?????24 ?????24 | ??????50 ??????10 ??????50 ??????0 |
3.) S
3(dosage 5882mg/kg.d) has tangible reduction effect to hemoglobin (HGB) and lipid peroxide value (LPO) in nicotine (0.54mg/kg.d) and tar (5.29mg/kg.d) the rising mice plasma, and to superoxide dismutase (SOD) the activity effect of being significantly improved of its reduction, experimental result sees Table 9:
HGB in table 9 mice plasma, the active variation of LPO and SOD
| Group | Time (d) | ????HGB?g/L | ???LPOμmol/L | The active u/g of SOD |
| Normal group nicotine group S 3+ nicotine tar group S 3+ tar group | ?????14 ?????14 ?????14 ?????14 ?????14 | ??128.714.7 ??151.5±8.9 ??125.4±6.7 ??157.0±6.5 ??129.0±9.5 | ??0.6478±0.2 ??2.814±1.37 ??0.4823±0.21 ??1.4465±0.59 ??0.9138±0.12 | ??504.5±46.2 ??418.4±7.8 ??436.3±15.3 ??209.6±17.7 ??342.7±25.3 |
4. Herba Sonchi Oleracei Sonchus oleraceus L., annual or biennial herb plant.Stem and leaf can be made feedstuff, and all herbal medicine has antitumor action.In the northern area of China also as the product of using with of Herba Patriniae.70% ethanol extraction of Herba Sonchi Oleracei is called for short S
4,, find that it has good anti-tobacco poisoning effect through experimentation.
Experimental result:
1) S
4The influence that causes chmice acute to poison to nicotine and tar.Mouse death rate is reduced to 10% and 10% by 50% respectively, and experimental result sees Table 10:
Table 10 S
4Influence to mouse death rate
| Group | Example number (n) | Dosage mg/kg | Time (h) | Mortality rate % |
| Nicotine group S 4+ nicotine group tar group S 4+ tar group | ???10 ???10 ???10 ???10 | ?????54.4 ??7267＋54.4 ????529.2 ??7267＋529.2 | ????????24 ????????24 ????????24 ????????24 | ??????50 ??????10 ??????50 ??????10 |
2.) S
4Protective effect to nicotine and each organ disease of tar induced mice
The nicotine model group: nicotine dosage 1.09mg/kg.d is after 14 days, and Mild edema appears in 80% animal liver tissue, the crowded and steatosis of the hepatic cords that has.10% animal kidney has blood stasis and lung large stretch of consolidation to occur.S
4Administration group dosage 7267mg/kg.d added nicotine 1.09mg/kg.d after 14 days, and the liver of 90% animal, kidney, lung, the heart, each internal organs of harmonization of the stomach spleen there is no unusually, had only 10% animal interstitial lung that the black particle calmness is arranged, and other each internal organs there is no unusually.
The tar model group, tar dosage 10.58mg/kg.d is after 14 days, and 90% animal liver Mild edema occurs or hepatic tissue has the black particle calmness.Tool taper coagulation necrosis focus and large stretch of consolidation appears in pulmonary and protein oozes out phenomenon under the 10% animal nephridial tissue tunicle.S4 pharmaceutical composition amount 7276mg/kg.d added tar 10.58mg/kg.d after 14 days, and each internal organs such as the liver of 80% animal, kidney there is no unusually.20% animal is slight hepatic cell edema or the black particle calmness is arranged only, and all the other internal organs such as kidney, lung there is no unusually.Experimental result shows S
4Cause the pathological changes of mice internal organs that good protective effect is all arranged to nicotine and tar.
3) S
4(dosage 7267mg/kg.d) has tangible reduction effect to hemoglobin (HGB) and lipid peroxide value (LPO) in nicotine (0.54mg/kg.d) and tar (5.29mg/kg.d) the rising mice plasma, and to superoxide dismutase (SOD) the activity effect of being significantly improved of its reduction, experimental result sees Table 11.
HGB in table 11 mice plasma, the active variation of LPO and SOD
| Group | Time (d) | ?????HGB?g/L | ???LPOμmol/L | ?????SOD?u/g |
| Normal group nicotine group S 4+ nicotine tar group S 4+ tar | ?????14 ?????14 ?????14 ?????14 ?????14 | ???128.714.7 ???157.0±6.5 ???139.5±10.6 ???145.3±9.3 ???101.7±11.7 | ????0.6478±0.2 ????2.814±1.37 ????0.8816±0.32 ????1.1566±0.6 ????0.67±0.26 | ???504.5±46.2 ???375.1±56.1 ???385.5±13.6 ???209.6±17.7 ???465.0±23.2 |
5. full leaf Herba Sonchi Oleracei Sonchus transcaspicus Nevski, herbaceos perennial about its purposes and economic worth and chemical constitution study situation, does not see that any report is arranged in the documents and materials at home and abroad so far.Full leaf Herba Sonchi Oleracei 70% ethanol extraction is called for short S
5, through experimentation, find that it has good anti-tobacco poisoning effect, especially anti-nicotine toxicity acts on very strong.
Experimental result:
1) S
5The influence that causes chmice acute to poison to nicotine and tar.Mouse death rate is reduced to 20% and 70% by 80% respectively, and experimental result sees Table 12:
Table 12 S
5Influence to mouse death rate
| Group | Example number (n) | Dosage mg/kg | Time (h) | Mortality rate % |
| Nicotine group S 5+ nicotine group tar group S 5+ tar group | ???10 ???10 ???10 ???10 | ?????122.9 ??7120＋122.9 ?????912 ???7120＋912 | ?????24 ?????24 ?????24 ?????24 | ??????80 ??????20 ??????80 ??????70 |
2.) S
5Protective effect to nicotine and each organ disease of tar induced mice
The nicotine model group: nicotine dosage 1.09mg/kg.d is after 14 days, and Mild edema appears in 80% animal liver tissue, the crowded and steatosis of the hepatic cords that has.10% animal kidney has blood stasis and lung large stretch of consolidation to occur.S
5Administration group dosage 7120mg/kg.d added nicotine 1.09mg/kg.d after 14 days, and the liver of all animals, lung, kidney, the heart, each internal organs of harmonization of the stomach spleen show no obvious abnormalities.
The tar model group, dosage 10.58mg/kg.d is after 14 days, and 90% animal liver Mild edema occurs or hepatic tissue has the black particle calmness.Tool taper coagulation necrosis focus and large stretch of consolidation appears in pulmonary and protein oozes out phenomenon under the 10% animal nephridial tissue tunicle.S
5Administration group dosage 7120mg/kg.d added tar 10.58mg/kg.d after 14 days, and the liver of 60% animal, lung, kidney, the heart, each internal organs of harmonization of the stomach spleen there is no unusually.40% animal only slight hepatic cell edema and cell rope is crowded, but each internal organs such as kidney, lung there is no unusually.Experimental result shows S
5Cause the pathological changes of each internal organs of mice that good protective effect is all arranged to nicotine and tar.
3.) S
5(dosage 7120mg/kg.d) has tangible reduction effect (be lower than or near normal value) to hemoglobin (HGB) and lipid peroxide value (LPO) in nicotine (0.54mg/kg.d) and tar (5.29mg/kg.d) the rising mice plasma, and to superoxide dismutase (SOD) the activity effect of being significantly improved of its reduction, experimental result sees Table 13:
HGB in table 13 mice plasma, the active variation of LPO and SOD
| Group | Time (d) | ????HGB?g/L | ????LPO?μmol/L | ?????SOD?u/g |
| Normal group nicotine group S 5+ nicotine tar group S 5+ tar group | ?????14 ?????14 ?????14 ?????14 ?????14 | ??122.7±5.4 ??151.5±8.9 ??125.6±4.9 ??145.3±9.3 ??123.9±6.2 | ????0.6478±0.2 ????2.814±1.37 ????0.3881±0.32 ????1.1566±0.6 ????0.6638±0.9 | ???504.5±46.2 ???375.1±56.1 ???482.4±16.3 ???209.6±17.7 ???440.4±21.3 |
Above-mentioned experiment shows that the ethanol extraction of sonchus Sonchus arvensis L. plant has very strong anti-tobacco poisoning effect.In like manner, the water extract of sonchus Sonchus arvensis L. plant also has very strong anti-tobacco poisoning effect.
Claims (1)
1. the application of sonchus Sonchus arvensis L. plant in the anti-tobacco poisoning medicine of preparation.
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| CNB001078186A CN1187061C (en) | 2000-06-22 | 2000-06-22 | Application of sowthistle in preparing tobacco toxin resisting medicine |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301722C (en) * | 2004-03-17 | 2007-02-28 | 上海市新文达生物科技有限公司 | Sow thistle extract, preparing process and application thereof |
| CN104905407A (en) * | 2014-03-11 | 2015-09-16 | 贵州省烟草公司贵阳市公司 | Drug-added filter-tipped cigarette |
| CN110038015A (en) * | 2019-05-21 | 2019-07-23 | 枣庄学院 | Application of the taraxasterol in the drug of preparation treatment nicotinism |
| CN111467382A (en) * | 2020-04-29 | 2020-07-31 | 段成路 | Green vegetable for nicotine-releasing and smoking-quitting and preparation method thereof |
| CN115568525A (en) * | 2022-08-29 | 2023-01-06 | 刘峰 | A herba Sonchi Oleracei tea beverage with tobacco toxin resisting effect, and its preparation method |
| CN116474020A (en) * | 2023-05-12 | 2023-07-25 | 北京暄芷达生物科技有限公司 | Sonchus arvensis jujube extract for improving sleep and application thereof |
-
2000
- 2000-06-22 CN CNB001078186A patent/CN1187061C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301722C (en) * | 2004-03-17 | 2007-02-28 | 上海市新文达生物科技有限公司 | Sow thistle extract, preparing process and application thereof |
| CN104905407A (en) * | 2014-03-11 | 2015-09-16 | 贵州省烟草公司贵阳市公司 | Drug-added filter-tipped cigarette |
| CN110038015A (en) * | 2019-05-21 | 2019-07-23 | 枣庄学院 | Application of the taraxasterol in the drug of preparation treatment nicotinism |
| CN110038015B (en) * | 2019-05-21 | 2022-03-15 | 枣庄学院 | Application of taraxasterol in preparation of medicine for treating nicotine poisoning |
| CN111467382A (en) * | 2020-04-29 | 2020-07-31 | 段成路 | Green vegetable for nicotine-releasing and smoking-quitting and preparation method thereof |
| CN115568525A (en) * | 2022-08-29 | 2023-01-06 | 刘峰 | A herba Sonchi Oleracei tea beverage with tobacco toxin resisting effect, and its preparation method |
| CN116474020A (en) * | 2023-05-12 | 2023-07-25 | 北京暄芷达生物科技有限公司 | Sonchus arvensis jujube extract for improving sleep and application thereof |
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| CN1187061C (en) | 2005-02-02 |
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