CN1329040C - Use of zijing in preparing preparation for treating shortsightedness - Google Patents

Abstract

The present invention relates to an application of animal eyes in preparation of medicines for treating myopia of human bodies, particularly to an application of a new medicine Zijing of traditional Chinese medicines in preparation of medicines for treating myopia. Zijing is fresh complete eyeballs of vertebrates, cattle and Ziyang healthy cattle or healthy fresh complete eyeballs of horses, donkeies and mules, and contains substances of various trace elements, multi-amino acids, proteins, multienzyme, polypeptide, ribonucleotide, vitamin, etc. by biochemical analysis, trace elements, vitamin and amino acid nutrient substances are the material basis of the metabolism of the structure functions ofeyes of the animal-Ziyang cattle, and is the material basis of treatment replenishment necessary for myopia, and cattle eyes which are products in biochemical factories and active substances directly participate in the metabolism of tissues of human eyes. The medicine of the present invention has the efficacy of nourishing visceral qi, nourishing blood and the heart, strengthening the spleen and eye muscles, relieving spasm, calming and clearing away heat, removing liver fire, improving eyesight, relieving complicated cataract, dissipating stasis, eliminating stagnation and blocking retrogression affection of eyes, and thereby, eyes are spirited, far and near things can be freely sawn, and the eyesight is recovered to the normal.

Description

The application of ZIJING in preparation treatment myopia preparation

Technical field

The present invention relates to the application of animal eyes in preparation treatment human body myopia preparation.

Technical background

In today of scientific and technical high speed development, bathomorphic research slowly not before, we think that key is to be that etiology is undecided, and are agnogenio, are difficult to execute and control.Emphasis is out of joint on research method.Etiology and pathogenesis is to diagnose originally the root of the rule of treatment, the source of determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs.

Western medicine and domestic doctor trained in Western medicine all have been limited in etiology on the genetic problem, are multifactorial inheritances and high myopia is autosomal recessive inheritance, AR as simple myopia, and " the genetic control opinion of myopia " stops the development of myopia studies.Academic state rests on the experimental study stage always, although understanding deepens continuously, achievement is very little, learning concept is still just smudgy, at present large hospital can only the mirror wildcarding correcting vision or operation rescue.Put on the PD that near-sighted glasses are also lost control of the student for the student, constantly increase with grade, the near-sighted number of degrees also continue to increase, and for the student, operation is rescued and is not indication, and it is still remote that prospect is captured in the myopia treatment.

And,, not only to see quality of medical care for the discovery and the invention of new drug at present at China student oculopathy-bathomorphic medical care problem, also to see the crowd's of being medically treated economic capability.China is developing country at present still, and the people, wage-earners are big, and generally income is few, is characteristics simply having adequate food and clothing, makes the receptible new drug of broad masses of the people must consider the product that cost is low, quality is high, just the medicine that numerous students are received treatment comprehensively.

Theory of Chinese medical science is the guide of holistic medicine, and whole the sight is that the traditional Chinese medical science is seen the marrow that the people knows the theory of disease, still thinks the exopathogenic factor authentication that combines with endogenous cause of ill from ancient times to the present, congenital defect, the day after tomorrow benefit.According to textual criticism, China's myopia is documented in " Records of the Historian " before 2000 to be had: " looking as hoping sheep " record.In 610 years Christian eras, in Sui's Chao Yuanfang (550-630 A.D.) " General Treatise on the Cause and Symptoms of Diseases order sick duke or princess under an emperor " there be note: " order can not hypermetropia, the impairment caused by overstrain internal organs is dirty, deficiency of liver-QI ".625 years Christian eras Tang's Sun Simiao " preventing and treating 16 of oculopathy " in have 10 with myopia relevant.End of the Ming Dynasty Fu Renyu " Precious Book of Ophthalmology " is pointed out: " Liver Channel deficiency, kidney channel disease ", " possess generate myopia ", " watching for a long time is hindered eyeball and become myopia " or the like illustrate that all " near-sighted etiology and pathogenesis " is due to environmental effect and the congenital defect cross interference.The physique constitution of students in 26 years of China, healthy investigation now found: constitutional myopia all betides child and teen-age growth and development stage, seldom morbidity behind the adult.This is relevant with the physiological characteristics of human body ocular structure emmetropization tendency fully, and constitutional myopia has 62.5% not have near-sighted family history; Have only 37.5% near-sighted family history is arranged; The heterozygote morbidity accounts for 17.5%; The high myopia number accounts for the 1-5% of total population number.Therefore we carry out having set up " environmental genomics " in the deep structure research of " environment and gene ", " gene and environment " under the overall situation influence of human genome, negate " absolute genetic control opinion " fully, judge that Chinese student's myopia is " can control the crowd ".

Summary of the invention

Technical problem to be solved by this invention is to propose ZIJING as the application of single new drug in preparation treatment myopia preparation, thereby provides that a kind of therapeutic effect is good, medical expense is low, the extensive bathomorphic preparation of the treatment of popularization and application.

The technical scheme that the present invention proposes is: as the application of Chinese medicine single new drug ZIJING in preparation treatment myopia preparation.

According to Chinese medicine title tradition nomenclature, use the place of production and medicinal part to the new drug standardize naming, the Ziyang buphthalmos is called for short: ZIJING.The place of production: ground such as Ziyang, Shaanxi, Shitai County, Anhui, enshi.

ZIJING is a vertebrates, the bright whole eyeball of the healthy cattle of Bovidae, Ziyang, or horse, donkey, the healthy bright whole eyeball of mule.

ZIJING is spherical or irregular spheroidal, diameter 2-3 centimetre, weight 60-80g, color aquatic foods, complete, sixth has semicircle born of the same parents' eyelid, fissura palpebrae, eyelashes before the eyeball, and black eyeball, Pupil god, tunica albuginea are arranged in the palpebral fissure, and back sixth body wall is softer, pliable and tough, surface is creamy white, smooth flexible, feeble QI is had a strong smell, and saline taste is arranged.

The ZIJING four seasons all can gather, and with fresh and alive eyeball extraction, the flush away bloodstain removes clean lipid foreign material on every side, wraps up, dries in the shade the freezing antistaling storing with Medulla Junci behind the salting when slaughtering an ox.Avoid with fiery baking, Exposure to Sunlight, wind in case break rottenly, the bright complete crack-free person of color is a good merchantable brand.Belong to ophthalmic remedy.

The ZIJING meridian distribution of property and flavor: sweet flat, salty, be slightly cold; The heart, liver,kidney,spleen.

ZIJING pharmic function: have tonification visceral-qi, nourishing blood and nutrient QI, suppressing the hyperactive liver to relieve the wind syndrome, liver heat removing and eyesight improving, blood circulation promoting and blood stasis dispelling, dredge the meridian passage, myopia change disease, sensible recovering lost eyesight.

The clinical practice of ZIJING: solely eyeball soup 60g is decocted in water for oral dose, clinical treatment myopia deteriorated case sudden blindness urgent need and as follows for the application of monarch drug prescription with the ZIJING:

ZIJING pharmaceutical formulation of the present invention has: Ziqing tranquilizing drug; Zijing spleen tonifying pill; Zijing kidney reinforcing pill; Zijing liver tonifying soup; Zijing stomach tonifying pill; Nebula removing pills; ZIJING FUMING YANTIE; ZIJING purification injection-YANSUTAI ZHUSHEYE.

ZIJING pharmaceutical formulation of the present invention:

1. injection: should meet " the regulation of two appendix 1B of Chinese pharmacopoeia version in 2000

2. tablet: should meet " the regulation of two appendix 1A of Chinese pharmacopoeia version in 2000

3. sugar pill: should meet " the regulation of two appendix 1H of Chinese pharmacopoeia version in 2000

4. Eye ointments: should meet " the regulation of two appendix 1G of Chinese pharmacopoeia version in 2000

5. eye drop: should meet " the regulation of two appendix 1J of Chinese pharmacopoeia version in 2000

6. powder: should meet " the regulation of two appendix 1P of Chinese pharmacopoeia version in 2000

7 capsules: should meet " the regulation of two appendix 1E of Chinese pharmacopoeia version in 2000

8. the transdermal eye pastes: should meet " the regulation of two appendix 1V of Chinese pharmacopoeia version in 2000

[scope of application]: near-sighted deteriorated cases such as all kinds of each phase myopia, developmental ophthalmocace, hypermetropia, astigmatism, stravismus, amblyopia, eyestrain, ocular injury, myopia complication, optic atrophy, non-suppurative keratitis, red film cyclitis, fluctuation body muddiness, central retinochoroiditis, myopia glaucoma, near-sighted cataract, ZIJING is the main flavor medicine of each disease treatment prescription of myopia.Good effect of the present invention can obtain explanation by following determination data and clinical conclusion.

One, the content assaying method of ZIJING of the present invention:

1. sensing equipment:

(1) SB--01 Atomic Absorption Spectrometer AAS.SOLAAR-M6 Tbermo jarrellashco. (USA)

(2) SB--23 chromatograph of liquid HPLC.LC-10A shimadzn (JAPAN)

(3) trade of SB--24 inductively coupled plasma boils instrument ICP-MSX7 Tbermo jarrellashco. (USA)

(4) SB--31 Hitachi 8350 automatic amino acid analyzers

2. bioassay standard

(1)GB/T5009.13-2003 (2)GB/T5009.14-2003 (3)GB/T5009.82-2003

(4)GB/T5009.84-2003 (5)GB/T5009.86-2003 (6)GB/T5009.90-2003

(7)GB/T5009.91-2003 (8)GB/T5009.92-2003 (9)GB/T5009.93-2003

(10)GB/T5009.123-2003 (11)GB/T5009.124-2003

3. measurement result

This product is Ziyang, a Shaanxi buphthalmos (abbreviation-ZIJING), and the bright product single of ZIJING new drug China Metrology Accreditation testing result is as follows:

(1) trace element:

1) calcium (Ca): 269.5mg/kg 2) ferrum (Fe): 45.8mg/kg 3) copper (Cu): 3.60mg/kg

4) chromium (Cr): 1.30mg/kg 5) manganese (Mn):＜0.002mg/kg 6) magnesium (Mg): 67.1mg/kg

7) sodium (Na): 7.56 * 10mg/kg 8) potassium (K): 391.9mg/kg 9) zinc (Zn): 5.81mg/kg

10) selenium (Se) 0.020mg/kg

(2) aminoacid:

1) aspartic acid (Asn) 1.12g/100g 2) threonine (Thr) 0.14g/100g

3) serine (Ser) 0.65g/100g 4) glutamic acid (Glu) 1.80g/100g

5) glycine (Gly) 3.24g/100g 6) alanine (Ala) 1.24g/100g

7) valine (Val) 0.41g/100g 8) methionine (Met) 0.19g/100g

9) isoleucine (Ile) 0.39g/100g 10) leucine (Leu) 0.73g/100g

11) tyrosine (Tyr) 0.31g/100g 12) phenylalanine (Phe) 0.52g/100g

13) lysine (Lys) 0.72g/100g 14) histidine (His) 0.20g/100g

15) arginine (Arg) 1.34g/100g 16) proline (Pro) 1.75g/100g

17) tryptophan (Trp) 0.19g/100g 18) cystine (Cys) 0.16g/100g

19) ammonia (An) 0.22g/100g 20) aminoacid summation 15.59g/100g

(3) vitamin

1) vitamin A 1.85 * 10mg/100g 2) vitamin B1 2.16 * 10mg/100g

3) vitamin B2 7.13 * 10mg/100g 4) vitamin B1 20.84 μ g/100g

5) vitamin E 1.68 * 10mg/100g

Two, provide 1, Beijing spectrum Buddhist nun physico-chemical analysis test center " survey report "

Detect: ZIJING

The bright full buphthalmos ball in Ziyang, Shaanxi

Sample state: solid-state

Temperature: 25 ℃

Inspection item:

Calcium (Ca): 269.5mg/kg ferrum (Fe): 45.8mg/kg copper (Cu): 3.60mg/kg

Chromium (Cr): 1.30mg/kg manganese (Mn):＜0.002mg/kg sodium (Na): 7.56 * 10 ³Mg/kg

Potassium (K): 391.9mg/kg zinc (Zn): 5.81mg/kg magnesium (Mg): 67.1mg/kg

Selenium (Se): 0.020mg/kg vitamin A: 1.85 * 10 ^-2Mg/100g

Vitamin B ₁: 2.16 * 10 ^-2The mg/100g vitamin B ₂: 7.13 * 10 ^-2Mg/100g

Vitamin B ₁₂: 0.84 μ g/100g vitamin C: do not detect

Vitamin E: 1.68 * 10 ^-1Mg/100g.Aspartic acid: 1.12g/100g threonine: 0.41g/100g

Serine: 0.65g/100g glutamic acid: 1.80g/100g glycine: 3.24g/100g

Alanine: 1.24g/100g valine: 0.41g/100g methionine: 0.19g/100g

Isoleucine: 0.39g/100g leucine: 0.73g/100g tyrosine: 0.31g/100g

Phenylalanine: 0.52g/100g lysine: 0.72g/100g ammonia: 0.22g/100g

Histidine: 0.20g/100g arginine: 1.34g/100g proline: 1.75g/100g

Tryptophan: 0.19g/100g cystine: 0.16g/100g aminoacid summation: 15.59g/100g

Three, provide 2, Beijing spectrum Buddhist nun physico-chemical analysis test center " survey report "

Detect: bright buphthalmos ball

Inspection item:

Calcium (Ca) 120.6 μ g/g zinc (Zn) 4.3 μ g/g copper (Cu) 1.0 μ g/g

Ferrum (Fe) 24.4 μ g/g potassium (K) 717.8 μ g/g magnesium (Mg) 46.5 μ g/g

Sodium (Na) 2337.2 μ g/g manganese (Mn) 0.45 μ g/g nickel (Ni) 5.9 μ g/g

Chromium (Cr) 1.9 μ g/g

Four, the inorganic constituents-trace element of ZIJING is treated bathomorphic physiology and pharmacological action analysis

1. the pharmacology analysis of trace element:

Chinese medicine ingredients and the drug effect analysis drug effect that integrates with, this is a kind of with molecular medicine, physiology, pathology, a cytohistologic innovation.Trace element is the material base of the human body cell function of macroscopical relatively macroelement, though trace element only is 0.05% of a human body weight, but it is in close relations with the physiological function of human body, the experiment proved that, the generation of child, the juvenile myopia of research growth and development stage, to develop, lapse to process all closely related with trace element, shortage as selenium, zinc, chromium, copper, manganese, calcium is relevant, so in control, must be noted that the ingredient of trace element, be that example is analyzed as follows apparently higher than the selenium in other place of production buphthalmos with trace element in the ZIJING:

(1) selenium: be to promote the multiple metabolic activity material base of ophthalmic, becoming human body to include the selenium total amount is 14-21mg, more with liver, pancreas, kidney content.Selenium is indispensable physiological function material in the human eye: selenium mainly has two kinds with the organic selenium compounds form in human body: the one, contain selenoamino acid, and another kind is a selenoprotein.Selenizing aminoacid mainly is selenizing cystine (Se-Cys) and selenomethionine (Se-Met); Most importantly glutathion peroxidase (HSH-Px) in the selenoprotein.Selenium is in the protein with two kinds of forms, a kind of disassociation factor, and another kind is the specific site performance.

1., the basic substance of selenium " visual acuity ": participating in the function of photoreceptor in the eye directly, participate in the conversion process of energy that photon conversion becomes the signal of telecommunication, thereby guarantee the vision normal function, is the basic substance of visual acuity.Hawkeye " visual acuity " higher position is because the selenium content height, bears in the eyes retina to contain selenium 700-800 microgram, and the normal eye contains 7 micrograms, and myopia takes place, develops, lapses to that retina selenium amount constantly reduces in the process, consequently causes blind.Closely related through laboratory report prompting myopia pathology and trace element.

2., selenium promotes the basic substance of the multiple metabolic activity of ophthalmic: selenocysteine is that the essential composition, particularly glutathion peroxidase (GSH-Px) of plurality of enzymes prothetic group is the destruction of the peroxidating ammonia pair cell that oxidizing process produces in the antagonist.Be to guarantee the important material of the normal operation of endocellular metabolism function.Selenium has obvious activation to the alpha Ketoglutarate dehydrogenase system.Promote the biosynthesis of coenzyme A, ubiquinone, so relevant with the electron transport of tricarboxylic acid cycle and respiratory chain.Tricarboxylic acid cycle is the substance metabolism hinge, is the last shared pathway of sugar, fat fat, the decomposition of aminoacid three major nutrient, is that acquisition maximum stages of energy are decomposed in three big material ammonifications, and the conduction function that directly affects one's power of vision has very important physiology of eye effect.

3., selenium has the anti-cell aging, the degenerative lesion that stops ocular structure: free radical is the one of the main reasons of human cell's aging, its main mechanism is lipid peroxidation, promptly in the presence of oxygen, free radical is an inducing agent, can bring out the inside and outside multiple biochemical component peroxidating of cell, makes the damage of cell membrane, cell interior 26S Proteasome Structure and Function, cause physiology, biochemical reaction disorder, show degenerative lesion.Therefore, allly free radical or polyphenoils can be eliminated, anti-senescence function can be stoped.Selenium by antioxidation and removing free radical, reduces or postpones the formation of lipofuscin just, thereby reaches the order ground of anti-cell aging and apoptosis.In ocular structure, glutathion peroxidase (GSH-P _x) be the important enzyme of anti-peroxidation, its major function is the formation that stops peroxide and free radical, selenium then is GSH-P _xImportant component part, 4 selenium atoms are arranged in each enzyme molecule.Contain selenic GSH-P _xHave extremely strong antioxidation, can make peroxide (ROOH) or H ₂O ₂Be reduced into nontoxic alcohol and water, just because of containing selenic GSH-P _xCan Cuiization H ₂O ₂Reduction reduces active oxygen, just makes the chance of oxidation unsaturated fatty acid and lipid reduce, and the generation of free radical just can be inexcessive, keeps the normal vigorous physiological metabolism of cell, reaches the purpose of anti-cell aging.

4., selenium in ocular structure to the histiocyte repair: selenium is the main component of peptide and enzyme in the metabolism, the effect with oxidation and removing free radicals, selenic antioxidative functional mass is glutathion peroxidase (GSH-P _z) main component; selenocysteine provides the necessary group in active center for this enzyme; the important component of glutathion peroxidase; catalytic reduction type glutathion becomes oxidized form of glutathione; utilize glutathion (GSH) to make virose peroxide be reduced to harmless hydroxy compounds; make oxide decompose, remove free radical, and protection membrane structure and function stop degenerative lesion.To stoping bathomorphic progress, promote reparation to play an important role, repair the molecule damage location.Selenium is the normal composition of eye muscle; can make eye muscle meat contraction convulsion and atrophy when lacking selenium, myocyte's compactness becomes, and lipid increases; calcinosis; cause into the muscular strength regression, selenium can quicken the structure cytothesis of regression, and the ciliary muscle cell is had protective effect; obviously suppress the non-enzymatic lipid peroxidation of eye muscle; stablize denatured areas inner cell and subcellular fraction film and alleviate muscle injury, dwindle the degenerating cell scope, therefore reach ocular tissue's cell, reach the purpose that stops regression and play reparation the regression disease.

5., selenic antiinflammatory action: selenium is that antioxidant also is an antiinflammatory, selenium GP particularly in antioxidation process _xLipid hydroperoxide and phospholipid hydrogen peroxide can be reduced, thereby gathering of free radical and active oxygen can be reduced.In Cycloxygenase and lipoxygenase pathway, GP _xCan reduce the intermediate product of hydroxide, thus the prostacyclin that inhibition causes inflammation and the generation of leukotriene.GP in addition _xCan also regulate respiratory burst by the generation of removing hydrogen peroxide and minimizing superoxides.Disease with oxidation reaction increase and inflammation-related all is subjected to the influence of selenium level.Experimental results show that: utilize bacterium colony in two bacteria culture medias (staphylococcus aureus and escherichia coli) to splash into to contain in the high natural tear of selenium the purification thing promptly be pure " lysozyme " drip to bacterium colony after 3 hours the bacterium colony dissolving disappear.In current experiment, find: purify in human tears liquid a kind of " lysozyme "; composition promptly is the higher solution of glutathion peroxidase content; relatively form is less for lysozyme in the tear and other lysozyme; very active; can be adsorbed onto in the crack of bacteria cell wall, " the enzyme pincers " of " lysozyme " directly vise the oxygen of two sugar rings in the antibacterial glycoprotein, thereby reduce bond energy; sugared key is broken very soon, and dissolving reaches the antiinflammatory purpose so antibacterial is dead.Glutathion peroxidase not only has anti-oxidation characteristics, also has the effect of anti-inflammation.

6., selenium and immunologic function: no matter from epidemiological study, or zoopery has proved that all selenium all has important relation to bathomorphic generation, development.Selenium is to biological fluid Immune Effects, also paid more and more attention.This has confirmed to have become indispensable element at the early stage selenium that human embryos are grown; it is except the composition as GSH-Px; outside removing free radical, protection cell membrane, nucleic acid, proteinic normal configuration and function; toxicity that can also antagonism heavy metal (hydrargyrum, lead, arsenic, cadmium, thallium) has Detoxication.Verify bright factually: utilize human peripheral lymphocyte and 10 ^-9～10 ^-6The selenium of M is cultivated together, finds that selenium can promote the generation of interferon, and in the external activity that can increase the r-interferon, strengthens the cytotoxicity of NK cells of human beings.We also find in the presence of sodium selenite, and the inductive mice spleen cell secretion of ConA 1L-2 increased activity highly significant is 2～3 times of matched group; Through the selenium pretreatment can spontaneous secretion 1L-2 generation.The secretion 1L-1 that selenium can also significantly increase macrophage is under the condition that LPS exists, and its activity of 1L-1 that adds sodium selenite is significantly higher than matched group under different diluting conditions.Human peripheral lymphocyte and mouse boosting cell PHA and ConA after the selenium pretreatment of low concentration induce the 1L-Z activity level obviously to raise; And find that concentration of sodium selenite is 3 * 10 ^-7During M, its facilitation is the strongest, and the 1L-2 activity level is 2 times of matched group.

Experimental results show that: selenium can also increase the secretion of immunoglobulin, leukocyte increasing is engulfed with sterilizing ability and is made the antigen of spleen adherent cell improve ability, though the effect of the no direct activation macrophage of sodium selenite itself, can not impel lymphocyte to produce macrophage activating factor (MAF) (MAF), but the synergistic activation macrophage is arranged and strengthen active anticancer with MAF, can remove low macrophage simultaneously lymphocytic inhibitory action.We find that selenium can activate peritoneal macrophage from the research that selenium is regulated the inductive cytotoxic cell of lymphocyte (LICC), the cell toxicant differentiation factor (CCDF) of its generation can with the activity of 1L-2 synergistic activation LICC; Also find simultaneously to use the internal energy activated macrophage of body and can promote the selenium dosage of its phagocytic function when experiment in vitro, not have the cytotoxic activity effect of significant short LICC, and the selenium of high dose has reversible inhibitory action to LICC.

NK cells of human beings is drawn as drawing a conclusion after active external influence and the Study on Mechanism thereof carrying out selenium:

1) concentration is the cytotoxic activity that the sodium selenite of 100-500ng/ml can obviously strengthen the NK cell;

2) imitate more height of target ratio, potentiation significantly;

3) selenium does not damage target cell membrane, does not see that activating PERIPHERAL BLOOD MONONUCLEAR CELL discharges more NK-cytotoxic factor (NKCF) yet.Think that selenium may be to promote both combinations by some structure that activates NK cell and target cell membrane surface, thereby strengthen the NK killing activity.Also observe selenium in addition and can enlarge the proliferation function of reorganization r-interferon (HnINF-r) the NK cell at body.Trace element has remarkable facilitation to immunity of organism.

Five, the analysis on trace of Chinese medicine:

Analysis on trace is to intert research project in each side such as medical material, process preparation, clinical practices.Past effective components of Chinese medicinal research biases toward Organic substance mostly, and less to the research of inorganic matter wherein.Since the eighties, along with people to the raising on the trace element effect understanding, the analysis on trace of Chinese medicine is also launched rapidly, mainly shows following several respects:

1. single medicinal material trace elements determination: find to contain in the composition macroelements such as various trace elements such as zinc, selenium, copper, chromium, manganese, calcium and sodium, potassium, magnesium during as the composition measurement of ZIJING, determine its status in treatment.How about the iron-holder of the Radix Polygoni Multiflori is measured the trace element that is higher than other, in the trace elements determination of Radix Ginseng, also finds to contain harmful substances such as harmful cadmium, antimony, bismuth, lead, therefore takes for a long time and will note appropriateness.

2. with the analysis on trace in the different places of production of a kind of Chinese medicine: as ZIJING must be that Ziyang buphthalmos content has therapeutic value, the treatment disease of can not being used as medicine that the content of other regional buphthalmos is extremely low.And for example the content of rubidium and manganese is the highest in the Rhizoma Gastrodiae, but the different place of production, contains the amount difference of trace element.Particularly rubidium, manganese, brill, lithium, molybdenum etc. think that this can be used as one of material base of estimating the Rhizoma Gastrodiae quality, hold concurrently and estimate the flavour of a drug quality.See the different places of production of ZIJING content elementary analysis table:

The different places of production bright buphthalmos ball micronutrient levels contrast table

Annotate: area, Ziyang buphthalmos is apparently higher than the content of area, Shanghai buphthalmos, and the manganese that eye function plays the interference effect is lower than Shanghai, the medicine source is gathered plays suggesting effect for we study the myopia treatment.

3. with clinical relevant effect applied research: if any the human atomic absorption spectrography (AAS) content of zinc, copper, ferrum in 40 kinds of emmenagogues such as Radix Angelicae Sinensis is measured and to be shown: iron content height such as Radix Morindae Officinalis, Radix Angelicae Sinensis; Rhizoma Polygonatis etc. contain the zinc height; Cupric height such as Fructus Schisandrae Chinensis.Pointed out the Chinese medicine the kidney invigorating, nourished blood, invigorated blood circulation, in close relations between the material base of Qi-tonifying drug and zinc, copper, ferrum and the human endocrine function.

The analysis on trace of Chinese medicine different parts 4. of the same race: as body, head, the tail of Radix Angelicae Sinensis, clinical function difference, its trace element is also different.Human energy dispersion X-ray analysis method is arranged, has measured the multiple tenor in Radix Angelicae Sinensis, body, the tail respectively, find that calcium in Qin's Radix Angelicae Sinensis, copper, manganese content are the highest, for the 1.5-6.8 that returns body or Radix Angelicae Sinensis doubly; Potassium in the Radix Angelicae Sinensis, iron content are the highest, for the 1.5-2 that returns head or return body doubly.The river Radix Angelicae Sinensis is also like this.It is all higher that each organ of Ziyang Niu Tizhong contains trace element, but have only Ziyang buphthalmos content to be fit to contain the various organic chemistry compositions of treatment human eye oculopathy.

5. cultivation and wild: comprise the research of trace element between synthetic and the natural drug.As wild animal and handling animal, contained trace element there are differences between wild plant and the cultivated plant.Most elements content becomes the positive benefit with place of production elements in Soil content (particularly active part).So, can consider to improve artificial culture or artificial breeding's quality by the supply measure of trace element.(trace element zinc, copper, chromium, calcium, manganese etc. see near-sighted basic research and applied research ZIJING, YANSUTAI, ZIJING prescription, new Chinese medicine Project Summary Report book for details)

Six, the organic principle of ZIJING: aminoacid, polypeptide, albumen, the pharmacological action analysis of enzyme in myopia is repaired

1. aminoacid and protein: the human proteinivorous final goal of the ultimate unit of aminoacid constitutive protein matter is to obtain needed each seed amino acid of body.

2. constituting the proteinic first step is that each aminoacid must link to each other with next aminoacid, forms a kind of chemical bond that is called peptide bond (peptide bond) between between an amino acid whose amino and another amino acid whose carboxyl.Side chain stretches out from carbon skeleton, the specific character of decision protein.

3. all protein are all formed because of aminoacid, and aminoacid can decompose component peptide and enzyme, combine with trace element and can form trace element for aminoacid or protein, form special protein, finish the specific function in the vital movement, therefore protein and all vital movements always connect together, and we can say the ribonucleic acid that does not have protein and link, and just do not have the existence of life.Known at present, needed by human six big class nutrient substance: i.e. protein, lipid, carbohydrate, vitamin, mineral and water.Wherein comprise the nutrient more than 40 kinds at least, each all can not lack, each is all important, but in six big class nutrient substance, what play special and centrality effect is protein, and proteinic function is numerous, can only set forth some representational parts at this, but also the multiformity that protein acts in vivo can be described, uniqueness and importance.Protein is called as the primary material of life, its significant feature:

1. the basic comprising material of body and all cells of body.

2. constitute the similar all life active compounds of body, comprise enzyme, hormones, antibody and immune material class, and the osmotic pressure that forms body, cause the comings and goings and the amino acid whose special role of body: methionine can form choline, acetic acid choline for methyl.As tryptophan is that the precursor methionine that nicotinic acid and 5-release tryptamines can form choline, acetic acid choline for methyl.Glycine forms important component of leaf woods, purine, pyrimidine or the like.

3. protein is a lot of important metabolites, the carrier of nutrient in the body, and for example multiple lipid, vitamin, mineral and trace element all need protein, or specificity protein carries and turns round.

4. thousands of various each proteinoid are made up of different aminoacid in the human body, and this is distinctive in the six big nutrient substance, is the demand material of vital movement.

5. support body growth to grow and the tissue renewal:

Protein is all life cells and the important composition of body fluid, is the main component that constitutes muscle, internal organs and the hormonal system of body, is the material base that body growth is grown and tissue repair is upgraded.The proteinic synthetic needs of cambium are amino acid whose without interruption.These heart tissues can be arranged in the embryo, and burn, hemorrhage or postoperative need replenish in the fresh blood in the child who is growing, child and the teenager, in the scar tissue of wound healing or in new life's the hair and fingernail.What more merge into background is we everyone proteinic effect in the body intracellular metabolic process all the time.The life-span of Red blood corpuscle has only 3-4 month, will be replaced by the new cell that bone marrow produces to certain hour.The cell that digestion is closed down can only be survived 3 days, and they are coming off of not stopping and upgrade.Skin Cell can death comes off, and new cell is by from following generation.Nearly all cell all will experience this generation, existence and dead process.In the cellular activity, the protein leaf of self constantly is being synthesized and is decomposing.Aminoacid in the food can be supported these all body growths and the process of organization component maintenance update.

6. synzyme, hormone and other chemical compounds:

Enzyme is to make one of important protein matter in the cell.Thousands of enzyme is just arranged in the individual cells, and each all is a kind of catalyst, and certain specific chemical reaction is carried out smoothly.

Enzyme is a catalyst: can make and originally can take place but carry out the very slow again response speed of speed and accelerate.Enzyme can make two compd As and B be in suitable position, reacts especially easily thereby make between the two.Compd A and B are absorbed into the active site of enzyme and are placed in and make the most incidental optimum position of reflection.Leave after generating new compd A B.

Single enzyme molecule can be finished the similarly synthetic reflection of hundreds of time in a second, some enzymes are arranged can be two or more products with compound decomposition or the atom of a chemical compound reset generate the another one chemical compound.Body is interior to be signaling molecule by a lot of hormones (hormone), wherein has some to come from aminoacid.Also there are some hormones to come from lipid.Multiple body of gland is secreted corresponding hormone according to the variation of internal milieu, and the reflection of hormone induction necessity is so that environment recovery is normal then.Thyroxin is the hormone that is made of aminoacid, is used to regulate organism metabolism.Insulin and glucagon are the opposite hormones of a pair of effect, are used for keeping the level of blood glucose.Also have much functions of other hormone various no less importants in participating in the adjusting body in the body.Have how much aminoacid to be connected to form the insulin of human body, and some side chain is how to attract each other to form insulin molecule with normal function in the left side according to certain principle.

Except the raw material as synthetic protein, aminoacid itself also has effect in vivo.For example, tyrosine is the ingredient of chemical messenger epinephrine and adrenalectomy element, and these couriers can transmit whole body nerve signal everywhere.Health also uses the tyrosine synthesis of melanin, and melanin is the source of skin, hair and eye color.Tyrosine also can change into thyroxine to regulate the metabolic rate of body.Tryptophan is the synthesis material of neurotransmitter serotonin and vitamin nicotinic acid.

7. synthetic antibody:

In the protein of organism all kinds, antibody (antibodies) can illustrate proteinic biologic specificity.Antibody can be discerned protein that belongs to self and the external source microgranule (being generally protein) of invading human body, and only can have an effect with the latter.Exogenous proteins may be the ingredient of antibacterial, virus or toxin, or causes certain composition hypersensitive in the food.Body identifies the protein of invasion, will produce to be used for suppressing this proteinic antibody specially.

Each antibody can only be used for destroying a kind of specific invador.A kind of antibody can effectively be resisted certain influenza virus, then can have no effect concerning the patient who has infected another kind of influenza disease.In case body produces certain specific antibody, will produce Memorability to this.When next time, same invador came to attack, he just can put up a resistance more rapidly.In other words, promptly human body has produced immunity to this invador.This molecular memory is exactly the principle of immunity inoculation, and immunity inoculation is promptly injected dead or the microorganism of inactivation or the immune defense system that its product comes human activin to people.Some immunity is lifelong, and for other some such as tetanus, then need the regular period inoculation once.

8. energy is provided:

Amino acid whose various function has only protein to realize, protein also can provide energy when being necessary.Human body be unable to do without energy constantly, and it is the most primary once obtaining energy.

1) when aminoacid is decomposed produce power, amino will be removed, and be used for other purposes or will be converted into carbamide (urea) by the liver absorption, be transported to kidney and get rid of with the form of urine.Remaining part is the same with fat with sugar, all form by hydrocarbon and oxygen, synthetic sugar and fat can be used to or in human body, glucogenic amino acid may be transformed with amino acid whose carbon skeleton, and and change into glucose and be utilized, or store with the form of glycogen, aminoacid also can transform ketogenic amino acid, and then transforms fat and be utilized.

2) aminoacid can not only provide energy, and much aminoacid can be converted into glucose, and this point fat is then not all right.Therefore under the condition of necessity, protein can help to keep stable blood sugar level, satisfies the needs of brain to glucose.

3) similarity of three kinds of materials that energy is provided and difference now should be very clear, and sugar provides energy, and fat provides high-energy, and protein provides energy and nitrogen under the situation of needs.

Aminoacid could can all be used for carrying out proteinic synthesizing when 4) having only sugar and the energy that provides of fat to be enough to satisfy the needs of cell.Human body can produce the chemical compound by sugar and depot fat energy, and wherein glucose is stored with the form of glycogen, and fat is stored with the form of triacylglycerol.And protein just exists with the constituent of tissue and the form of functional molecular.When the situation is critical, the human body break-up tissue protein of just having to obtained aminoacid and obtains energy.Calling of every kind of protein all has certain order: at first be the small protein matter in blood and the liver, next is only the protein of muscle and other organs.Therefore energy shortage (hunger) then can be both the consumption that causes fat and body non-fat tissue.

5) human body can't store the acid of excess of ammonia base, at this moment has only its amino is sloughed, and remaining carbon skeleton is converted into energy storage goods and materials hepatic glycogen and fat.

9. keep body fluid and electrolyte balance:

Protein can help keep the balance (fluid and electrolyte balance) of body fluid and electrolyte by the amount of each several part liquid in the control agent.Must keep certain amount of fluid in the living cells, too much liquid may cause cell to burst, and may make the cell loss of function at least and cross.Although water can pass in and out cell freely, but protein is not all right; And protein is possess hydrophilic property, so cell can be kept their required liquid by keeping the interior protein of born of the same parents and the storage capacity of some other mineral.Cell can also keep the constant liquid volume of iuntercellular by secreting protein and anti-material, so just can keep inside and outside suitable balance.If this cover system is not normal, the extracellular will accumulate a large amount of body fluid and cause edema (edema).

Except the amount of body fluid, the composition of body fluid also is extremely important to life.Transport protein on the cell membrane is by constantly with various sending material out or transport cell and keep body fluid and form.Such as, sodium is higher in extracellular concentration.Will damage the function of heart, lung and brain if this balance is damaged, cause serious acute disease.Protein in the cell is suppressed at and takes great pains to make liquid and electrolyte to maintain suitable ratio, avoids this calamitous situation to take place.

10. keep acid-base balance:

Health constantly produces acid (acids) and alkali (bases) under the normal condition, must be transported to Excretory organ by blood.Blood must get off to finish this work in the prerequisite that does not influence self acid-base balance (acid-base balance).Proteinic another function in Here it is the blood, he can be as the buffer substance (buffers) that keeps the normal pH value of blood, and it can collect too much hydrion (acid), and can when hydrion is very little it be discharged.Its mechanism just is that amino acid whose side chain is electronegative, can hold the hydrion of another positively charged when being necessary.

Blood pH makes one of condition that is strictly controlled in the body.If it is excessive that pH value changes, will cause dangerous acidosis (acidosis) or opposite alkalosis (alkalosis), cause stupor even dead.Why danger is that they can exert an influence to protein to this situation.When proteinic buffer capacity has reached capacity, promptly the hydrogen atom of Jie Shouing reached that they can accept to greatest extent the time, too much acid will make their degeneration, thereby a lot of processes of health are damaged.Summary has also been replenished our proteinic various functions previously discussed.See Table:

The summary table of table protein function

(1) growth and renewal: protein is as the structure material of physical growth and tissue repair.(2) enzyme: protein promotes that required chemical reaction carries out smoothly.(3) hormone: protein is regulated the various physiological process of health.(4) antibody: protein forms immune system molecule resist the disease.

(5) body fluid and electrolyte balance: distribution and mineral that protein helps to keep each several part body fluid are formed.(6) acid-base balance: protein helps to keep the acid-base balance of various part body fluid as buffer agent.(7) energy: protein can provide the energy that needs for health.(8) transportation: the various materials that protein helps the body transport point to need, as fat, mineral and oxygen.(9) blood pool: protein provides rack to form for clot in the above.(10) constituent: protein is most of health mechanism, as the core of skin, muscle tendon, ligament, film, muscle, organ and skeleton.

Generally speaking, the aminoacid in the cell can be used to: 1. synthetic protein; 2. be converted into other little nitrogen-containing compounds, as nicotinic acid; 3. be converted into other aminoacid.Therefore the function of nutrition, regeneration, reparation is played in the effect of ZIJING in the myopia treatment.(seeing near-sighted basic research and applied research ZIJING, YANSUTAI, ZIJING prescription, new Chinese medicine Project Summary Report book for details)

Seven, the vitamin of ZIJING physiological and pharmacological function analysis in near-sighted human body:

Vitamin is to keep the necessary class organic compound of human body normal activities, and its content is atomic in vivo, but plays an important role in processes such as the metabolism of body, growth, growth.Though their chemical constitution and character are not close, and common feature is arranged: 1. all with vitamin itself, or the form of the precursor compound (having another name called the quality of supporting one's family) that can be utilized by body is present in the natural food; 2. non-housing construction composition does not provide heat energy yet, but is undertaking special metabolic function; 3. generally can not synthesize in vivo, or synthetic quantity (vitamin D exception) very little, must provide or medicine replenishes by food; 4. human body only needs minute quantity to satisfy, but must not lack.Lack certain vitamin to a certain degree, can cause avitaminosis.Just show multivitamin in the occurrence and devlopment of myopia process and lack feature, the discovery of ZIJING ocular structure just needs the additional medicine of multivitamin in a large number.

Good effect of the present invention is conspicuous, ZIJING is through biochemical analysis, contain various trace element, several amino acids, protein, multienzyme, polypeptide, nucleotide, materials such as vitamin, trace element, vitamin, plain this three big element of amino acid nutrient, it is the material base of animal Ziyang the eye splice structure functional metabolism, it also is the material base that the essential treatment of myopia replenishes, the tissue metabolism that the product of this bioid factory of buphthalmos and active substance are participated in human eye directly, can mend visceral-qi, nourishing blood and nutrient QI, the spleen invigorating of nourishing heart, strong eye muscle, slow spasm, flat sweet flame-out, liver heat removing and eyesight improving, black expense is moved back, stasis of blood block removing is fallen, stop eye to stop degenerative lesion, refreshing light is eagle-eyed, and brilliance is sent out more at a distance, look hypermetropia closely freely, vision restoration as before.(seeing near-sighted basic research and applied research ZIJING, YANSUTAI, ZIJING prescription, new Chinese medicine Project Summary Report book for details)

Eight, ZIJING purification liquid a--cave, eye district is annotated ZIJING purification liquid YANSUTAI ZHUSHEYE treatment myopia myopia and is cured that the back is capsulociliary to dynamic observe report

We adopted in 2002 UBM to a cave, 68 example eye district annotate YANSUTAI (eye is bright) treatment myopia cure the case history drug withdrawal carry out after 3 years looking of eye near, look adjusting far away and dynamic observe, look near, as to look far different corpus ciliare shaped position under spontaneous adjustment state dynamic change and do detailed observation, the model of action of Suspensory ligament when being intended to understand the living human eye adjusting of myopia healing back indirectly.And assessment Chinese medicine ZIJING prescription and ZIJING purification liquid YANSUTAI (eye is bright---and ocular tissue's structure behind the injection for treating, muscular strength, muscle group are reported as follows curing the changing condition that eye recovers:

One, clinical data

In January, 2000 to 2004, clinical myope's 1328 examples of controlling altogether of the bright injection of eye were annotated in year cave, eye district in April, totally 2608 eyes, (including 48 single myopia), wherein simple myopia 1172 examples, 2296 eyes, pathological changes myopia 156 examples, 312 eyes.Male's 608 examples, 1184 eyes, women's 720 examples, 1424 eyes.Age 7-22 one full year of life, the course of disease is the shortest 5 months, and is the longest 12 years.Calendar year 2001 to 2003 year carries out patient's randomization of stopping controlling followed the trail of and checks 192 examples, 384 eyes.Cure randomization 68 examples (68 eyes) among patient in various each myopia, male's 30 examples wherein, women's 38 examples phase.The right eye refractive diopter is-4.50-0.00D average (1.47 ± 1.39) D: left eye-3.00-0.00D, average (1.28 ± 1.20) D.All get left eye and observe, 28 emmetropiaes of Zhi Yuing wherein, 40 after treatment, low myopia is still arranged, the myopia of distant vision more than 1.0 of correcting defects of vision.Observed personnel must get rid of other ophthalmics of existing trouble, all kinds of eye table inflammation, astigmatism, amblyopia, hypermetropia, cornea illness, glaucoma, cataract and iridocyclitis etc., and ban use of ciliary muscle to regulate the individuality of medicine such as atropine, pilocarpine etc.

Two, observational technique

1, instrument: adopt U.S. Paradigm ^TMUBM P40 type ultrasound biomicroscopy UBM, transducer frequency 50MH ^z, probe side direction and axial resolution are 50m, seek and visit the about 5mm of the degree of depth and width, monitor indication range 5mm * 5mm, (864 pixels * 432 pixels).

2, inspection method: under the adequate illumination, the detected object right eye is with 0.4% tetracaine topical anesthesia, and lying on the back lies low, and the built-in suitable eyecup of conjunctiva of right eye capsule (diameter 20mm or 22mm) is annotated an amount of 0.9% contact with normal saline agent in it, and it is vertical with eyeball to adjust probe.Deceive " E " as looking far, look near fixation object with the white background of length of side 4cm and 0.4cm respectively.Look steadily that person under inspection's head is active, eyes turn left, watch attentively and oblique angle, concordant upper left side, eye position about 45 ° and 5m distant place (theoretical infinite point) hypermetropia mark with left eye, absorb 9 o'clock of right eye, 2 of orientation corpus ciliare cross-sectional images (look 1 figure far away and both looked 2 figure far away) with UBM under (minimum) adjustment state; Look steadily the person under inspection again and watch equal angular self near point place myopia mark attentively, with 2 of corpus ciliare cross-sectional images (look nearly 3 figure and look nearly 4 figure) under nearly (maximum) adjustment state of method picked-up.All inspections are independently finished by the author.

A) measuring method: the place image is by pyromagnetic printer prints, AGFA SnapScan e20 scanner is with 200% scanning of optical resolution 300dpi zoom, with 3D-DOCTOR3.5.011019 image analysis software it is carried out the measurement of a plurality of two-dimentional variablees, each variable is surveyed and is averaged for 3 times.All measurements are independently finished by the author.Measure content and comprise 18 parameters (Fig. 1)

Three, statistical method

The result imports SPSS10.Ofor windows statistical package, the partial Correlation Analysis in one factor analysis of variance, the variance analysis of many dependent variable linear model and the binary variable correlation analysis between each variable before and after regulating.

Marginal data: CBT1: ciliary body thickness 1[4], 1.0mm is perpendicular to the ciliary body thickness of sclera inner surface behind the scleral spur.CBT2: ciliary body thickness 2[4], 2.0mm is perpendicular to the ciliary body thickness of sclera inner surface behind the scleral spur.CBT3: ciliary body thickness 3,3.0mm is perpendicular to the ciliary body thickness of sclera inner surface behind the scleral spur.CBT4: ciliary body thickness 4,4.0mm is perpendicular to the ciliary body thickness of sclera inner surface behind the scleral spur.CPD ': ciliary processes length, 1.5 places are to the distance of the interior point of ciliary processes behind the scleral spur.SC: scleral spur ciliary processes distance, scleral spur is to the vertical dimension of CPD '.CT: the ciliary processes width, cross a divide a word with a hyphen at the end of a line straight line of making vertical CPD ' and another edge of ciliary processes of ciliary processes iris and intersect at a point distance between two points.TCPD ': girder ciliary processes (interior point) distance [3], scleral spur preceding 500 is to the vertical dimension of interior point and iris major axis parallel lines.ICPD ': iris ciliary processes (interior point) distance, TCPD ': the distance of point in iris and the ciliary processes on the line.Angle, AOD500:500 room open distance.500m intersects at a point in iris perpendicular to corneoscleral junction inner surface one straight line before the scleral spur, distance between two points. θ 1: girder iris angle [3], angle between 2 difference of crypts of Fuchs and AOD500 line, θ 4: sclera ciliary processes angle [3] sclera outer surface tangent line and ciliary processes angle.θ 3: sclera iris angle [3], sclera outer surface tangent line and the iris angle [3] between line respectively of dividing a word with a hyphen at the end of a line.θ 5: iris ciliary processes angle, iris major axis and ciliary processes angle.θ C: point becomes angle and gauffer portion flattening portion point of interface in the ciliary processes, a ciliary processes iris difference line angle of dividing a word with a hyphen at the end of a line.AE: corpus ciliare cross-sectional area between the 4mm behind the scleral spur.Ar: ring muscle area.

By the ciliary processes iris divide a word with a hyphen at the end of a line a little be parallel to the sclera inner surface to gauffer portion flattening portion point of interface one line with interior ciliary processes area.A1 longitudinal muscle area is last both poor.

Four, observed result

1, controls back eye and look far away, ciliary region UBM image (Fig. 2) when looking nearly adjusting

By the UBM image as seen, controlling back eye, to look when far away corpus ciliare gauffer portion more smooth, and thickness and par are as good as, the shorter broad of ciliary processes, point is more blunt in it, far away with iris distance, angle of anterior chamber broad: look corpus ciliare gauffer portion is higher than the par obviously to eye axis protuberance when near, ciliary processes is elongated to narrow down, interior point becomes right angle even acute angle, and close iris before revolving, and the angle of anterior chamber degree of opening slightly diminishes.Look near and look when far away, iris, sclera have no significant change.

2, eye is looked far away, each parameter variation (table 1) of ciliary region when looking nearly adjustings

Table 1 treatment back eye corpus ciliare is being looked far away and is being looked nearly (the x ± s) of form location parameter under the state

Parameter	1 #	2 #	3 #	4 #
					Morphological parameters
CBT1(mm)	0.7688±0.1375	0.7790±0.1462	0.8550±0.1358	0.8693±0.1333
					CBT2(mm)	0.5256±0.1221	0.5133±0.1146	0.4965±0.1063	0.5103±0.1031
CBT3(mm)	0.3297±0.0872	0.3317±0.0720	0.3109±0.0705	0.3155±0.0699
					CBT4(mm)	0.2075±0.0413	0.2011±0.0404	0.1989±0.0460	0.2059±0.0445
CPD′(mm)	0.9535±0.1981	0.9571±0.1908	1.2816±0.2698	1.2847±0.2656
					CT(mm)	1.0454±0.3671	1.0296±0.2959	0.7794±0.1816	0.8140±0.2091
θc(mm)	126.9026±16.6904	124.8076±17.1408	92.2314±20.9929	93.5065±21.6779
					Ae(mm)	168219.4819±24104.9060	169591.8342±250748681	181926.5551±26083.0017	187162.3709±22456.7092
Ar(mm)	61718.3832±14384.5223	59832.3848±155512097	72125.0888±16953.3823	74249.9109±18144.8019
					Al(mm)	106574.6301±16149.7594	109759.4347±16878.1197	109757.3486±15839.3728	111358.4895±18819±9213
Location parameter
					SC(mm)	1.2152±0.1692	1.2240±0.1562	1.0042±0.1871	1.0188±0.1966
TCPD′(mm)	1.5550±0.2432	1.5754±0.2053	1.3827±0.2034	1.4134±0.2219
					ICPD′(mm)	0.5669±0.2391	0.5398±0.1425	0.4007±0.1687	0.4057±0.1787
θ4(°)	72.1049±12.5643	69.9288±11.8916	54.6132±10.2557	55.8938±11.2494
					θ3(°)	44.0241±8.4572	43.0006±8.4319	44.2738±7.6117	43.5121±8.6276
θ5(°)	28.0853±11.7925	26.9293±12.2258	10.3394±10.6269	12.3817±12.3933

Annotate: * is for looking 1 figure far away, and 2 for looking 2 figure far away, and 3 for looking nearly 3 figure, and 4 for looking nearly 4 figure

(1) change (table 2) of corpus ciliare form position:

Parameter	The Tamhane check ##						The SNK check ##
									1-2	1-3	1-4	2-3	2-4	3-4	1-2	3-4
	Morphological parameters
CBT1										0.999	0.002 #	0.000 #	0.012 #	0.001 #	0.990	0.669	0.547
	CBT2	0.991	0.598	0.967	0.941	1.000	0.970	0.427
CBT3										1.000	0.670	0.877	0.435	0.705	0.999	0.371
	CBT4′	0.931	0.823	1.000	1.000	0.986	0.935	0.646
CPD′										0.929	0.000 #	0.000 #	0.000 #	0.000 #	0.938	0.929	0.938
	CT	0.737	0.000 #	0.000 #	0.000 #	0.000 #	0.460	0.737	0.460
θc										0.978	0.000 #	0.000 #	0.000 #	0.000 #	1.000	0.526	0.699
	Ae	1.000	0.011 #	0.000 #	0.034 #	0.000 #	0.760	0.744	0.212
Ar										0.976	0.001 #	0.000 #	0.000 #	0.000 #	0.981	0.500	0.448
	Al	0.840	0.819	0.517	1.000	0.996	0.995	0.357
Location parameter
									SC	1.000	0.000#	0.000#	0.000#	0.000#	0.998	0.775	0.631
TCPD′	0.996	0.000 #	0.003 #	0.000 #	0.000 #	0.955	0.586	0.415
									CPD′	0.964	0.000 #	0.000 #	0.000 #	0.000 #	1.000	0.396	0.873
θ4(°)	0.884	0.000 #	0.000 #	0.000 #	0.000 #	0.982	0.271	0.517
									θ3(°)	0.980	1.000	1.000	0.929	1.000	0.995	0.807
θ5(°)	0.994	0.000 #	0.000 #	0.000 #	0.000 #	0.887	0.567	0.312
									The angle, room
AOD500	0.948	0.016 #	0.012 #	0.014 #	0.010 #	0.918	0.948	0.918
									θ1	1.000	0.455	0.637	0.407	0.593	1.000	0.324

Annotate: * show look far away, look difference has statistical significance when near.1,2,3,4 represent to look far, look nearly each 2 figure respectively, 1-2,3-4 etc. are respectively make comparisons * * Tamhane and SNK check of parameter between two figure and are the common method of one factor analysis of variance in the SPSS statistical software, the former is used for variance nonhomogeneity person, and the latter is used for the data of homogeneity of variance.

Look far away 1,2 figure and look between nearly 3,4 figure at 68 eyes, the ANOVA that carries out capsulociliary morphological parameters CBT1, CBT2, CBT3, CBT4, CPD ', CT, θ C, Ae, Ar and AL between each group analyzes.The result shows: (one), be subjected to an inspection eye CBT1 to look nearly ratio to look the obvious increase that has far away, and looking nearly ratio, CBT1 looks the obvious increase that has far away, and CBT2, CBT3, CBT4 do not have obvious change, corpus ciliare when regulating mainly be behind the scleral spur before the 1mm place gauffer portion part thicken, and 2,3 and the 4mm place be that the par does not have obvious thickening.(2) looked closely with looking far by inspection eye and compare, CPD ' obviously increases.CT and θ C obviously reduce, and ciliary processes becomes sharp-pointed and long and narrow when promptly regulating.(3) be subjected to inspection eye Ae and Ar to look obvious increase far away looking nearly ratio, A1 but changes not quite, illustrate when regulating that the corpus ciliare cross-sectional area increases before the 4mm, and especially the ciliary teat promptly encircles formation flesh place, and the stringer fiber does not then have obvious change.The ANOVA of 68 example treatment back eyelash shape location parameter SC, TCPD ', ICPD ', θ 4, θ 3 and θ 5 the analysis showed that between 4 groups, look near and look more than, except that θ 3, all the other all diminish, it is the distance of ciliary processes and scleral spur, trabecular reticulum (500m before the scleral spur), iris, all significantly reduce with sclera and iris angle, illustrate when regulating and revolve before the apparent reach of JIANMING in the ciliary processes.And sclera iris angle does not have obvious change, and iris is not seen obvious displacement.The relatively variation of angle of anterior chamber parameter A OD500 and θ 1 between 4 groups.Look nearly ratio and look AOD500 reduces when far away, 1 no significant change of θ, promptly angle, 500m room open distance is existing reduces, and girder iris angle does not still have obvious change, and the anterior chamber slightly shoaled when near the adjusting be described, and angle, room degree of opening is decline slightly.Look far away, look and carry out the variance analysis of many dependent variable linear model between nearly adjusting relatively, draw above-mentioned identical result.

(2) corpus ciliare form position correlation analysis research: each form location parameter of corpus ciliare is made correlation analysis, the result shows: when (one) looks nearly adjusting, there is not significant correlation near the displacement of iris and the increase of anterior thickness of corpus ciliare and ciliary processes length forward between in the corpus ciliare, and with the degree height correlation of each position parameter change, each location parameter change in elevation unanimity (table 3).

Table 3 corpus ciliare TCPD ' change Related factors

Test value	ICPD′	CBT1	CPD′	SC	CT	AOD500	θ4	θ5
									r t p	0.489 4.553 0.000*	-0.161 0.191	0.176 0.152	0.702 8.017 0.000*	0.514 4.865 0.000*	0.262 2.201 0.031*	0.672 7.377 0.000*	0.492 4.590 0.000*

Annotate: the different significance of * differential

(2) the come to a point growth of degree and the anterior thickness of corpus ciliare, ciliary processes length of point in the ciliary processes with its high-positive correlation that reduces to scleral spur distance and sclera angle, and increases along with the degree of area increase; But itself and ciliary processes width, to the distance no obvious dependency (table 4) of girder

Table 4 treatment back eye ciliary processes becomes angle θ c Related factors

Test value	CBT1	CPD′	SC	CT	TCPD′	θ4	Ar
								r t p	0.340 2.934 0.005*	0.693 7.818 0.000*	0.275 2.327 0.023*	0.023 0.854	0.172 0.161	0.392 3.464 0.001*	0.307 2.620 0.011*

Annotate: the different significance of * differential

(3) the corpus ciliare front portion is thick more, ciliary processes is more long and sharp, and its annular pars muscularis area increase is many more; But ciliary processes is narrow more, and this area increase is few more; In itself and the ciliary processes point before revolve and do not have obvious relation (table 5)

Table 5 treatment back eyelash shape bulk area Ar Related factors

Test value	CBT1	CPD′	TCPD′	SC	CT	θc
							r t p	0.499 4.678 0.000*	0.444 4.021 0.000*	-0.129 0.296	-0.041 0.737	-0.316 -2.705 0.009*	0.307 2.620 0.011*

Annotate: the different significance of * differential

When (four) regulating thickening, TCPD ' and the θ 4 of the degree that shoals of angle of anterior chamber and CBT1 and CPD ' reduce be high-positive correlation (table 6), the variation of angle of anterior chamber was relevant with the change of corpus ciliare form and position when adjusting was described.

Table 6 treatment back angle of anterior chamber changes the Related factors of (AOD500) when regulating

Test value	CBT1	CPD′	TCPD′	SC	CT	θ4	θ5
								r t p	0.344 2.984 0.004*	0.320 2.744 0.008*	0.262 2.201 0.031*	0.236 0.053	0.009 0.945	0.275 2.326 0.023	-0.067 0.587

Annotate: * differential significance

The 5th observes brief summary

One, test is looked in limited distance far, is looked near spontaneous adjusting and carried out preliminary study, sclera, iris, axiallength when we also can further understand adjusting controlling the back human eye ^[5]Effect and anterior chamber's change ^[6]Directly observe corpus ciliare, crystalline lens, Suspensory ligament system ^[3]Delicate contact.Recovery extent with checking treatment back ocular structure.In addition, regulate front and back collarette temporo side and each parameter of nasal side and variation thereof obvious difference is arranged ^[7], further research is regulated and the relation of gathering.Centuries, the eye regulatory mechanism is the focus of research arguement and bone of contention always, mainly concentrates on when regulating on loose still nervous this problem of Suspensory ligament.The invention of ultrasound biomicroscopy UBM has remedied the deficiency of the poor definition of common A, B ultrasonic imaging.Can on living human eye, become microscope noinvasive observing effect with low power.The three-D ultrasonic intravital microscope shows the exploitation that picture is checked ^[8], improved the visual degree of spatial relationship, also the further research for anterior ocular segment anatomical structure and regulatory mechanism provides new route, also lays the first stone for the ocular structure live body dynamic observes.

This test is with UBM observation and analyze the spontaneous hypermetropia of looking of treatment back human eye when closely regulating, the variation of a plurality of parameters of collarette structure, the result shows, each muscle fiber of corpus ciliare is coordinated to shrink when looking near adjusting, mainly is that ring muscle shrinks, and makes it forward to intrinsic displacement, ciliary processes is revolved before coming to a point, near crystalline lens, thereby Suspensory ligament is loose, and angle of anterior chamber narrows down.This result of study is supported the loose theory of adjusting of Helmholtz.This is that major step is advanced in the research of myopia therapeutics again.Also be to the provide theoretical foundation of treatment antifatigue " pharmacological action of ZIJING " with " bending the degree adjusting says ".

The change of corpus ciliare form position when two, regulating

Human eye after the healing and normal eye contrast sees that curing the back human eye looks when near from looking far to become, and refracting media need increase refractive diopter and increase entering ray refraction and convergence, makes the clear retina that focuses on of divergent rays.Primate, as the Rhesus Macacus and the mankind, this process is mainly finished by the variation of corpus ciliare-Suspensory ligament-intraocular lens system: look the contraction of ciliary muscle when near, changed the tension force of Suspensory ligament, because the plasticity of the elasticity of crystalline lens self cyst membrane and substrate makes its anteroposterior diameter increase the obvious prolapse of front surface to the crystalline lens tractive, radius of curvature reduces, therefore refractive diopter increases: look ciliary muscle diastole when far away, and the above-mentioned change of driving in the wrong direction, refractive diopter diminishes.

Because ciliary processes bottom (2mm) accounts for whole corpus ciliare length 1/3, the 1mm place is equivalent to the most outstanding place of corpus ciliare gauffer central authorities of portion behind the scleral spur, and 2,3, the 4mm place is equivalent to its par.Look when far away, gauffer portion is more flat, and ciliary processes is short and wide, and interior point is more blunt, and similar one is that base, interior point are the obtuse triangle of drift angle with the sclera; It is inwardly outstanding to look when near gauffer portion, ciliary sudden change long and narrow, interior point also becomes right angle even acute angle, as one be the base with corpus ciliare near the leading edge of iris, sclera is the right angled triangle of opposite side, ciliary processes is outstanding more, point is also sharp more in it.

Look when far away, ciliary processes is apart from each index point, and is all far away as trabecular reticulum and the iris of 500m before scleral spur, the scleral spur.Sight line draws near, and ciliary processes is obviously near above-mentioned sign, even is close to iris, disappears with the distance of iris, also obviously diminishes with the angle of sclera outer surface tangent line, when proving absolutely nearly adjustings, ciliary processes especially its interior point really pro-revolve reach.During ciliary muscle contraction because sclera inner surface concave surface is inside, simultaneously before stop scleral spur compliance less than back stop ora serrata compliance, so corpus ciliare integral body is promptly along the amplitude displacement forward of inner surface bending, its result must be inside (axis oculi) moving forward.Ciliary muscle contraction is strong more, and ciliary processes is more long and sharp, also the closer to iris and scleral spur.

Since capsulociliary forward, move inward, JIANMING is apparent in when shrinking owing to corpus ciliare again inwardly gives prominence to, no matter the equator of lens diameter how, distance is between the two obviously dwindled, the Suspensory ligament that is connected between the two is loose, loosened the outside traction force of Suspensory ligament to equator of lens, further finished the function of adjusting, this also is the theory that meets the loose theory of Helmhotz fully.The adjusting of treatment back eye is regulated with normal eye function fully has relevant concordance.

We also observe in the test, look when near the angle of anterior chamber degree of opening and slightly dwindle, and girder iris angle does not narrow down as yet, are not enough to cause that angle, the such tangible room of similar angle closure glaucoma narrows down or closes.Being rotary with certain dependency before the elongated and reach of this minor variations and ciliary processes, may be because capsulociliary contraction tractive scleral spur has due to the displacement trend backward.

The contraction of ciliary muscle when three, regulating

During near the adjusting, corpus ciliare front portion (CBT1) is thickening obviously, this is 3 groups of the most concentrated places of muscle fiber just, ciliary processes rises appreciably and comes to a point, just be the circular fiber place: corpus ciliare rear portion (CBT2, CBT3, CBT4) but do not have significant change, and the main distribution stringer fiber of this section has not had circular fiber.In addition, the corpus ciliare cross-sectional area is looked obvious increase far away looking nearly time ratio, and the part of increase mainly is the inwardly outstanding part of gauffer portion, the place of ring muscle fiber just, the amplitude of its increase also with above-mentioned change high-positive correlation; As and if the stringer pars fibrosa does not have obvious change, revolve before the change of ring muscle contraction simultaneously and the reach of ciliary processes to have no relations yet.This shows that each muscle fiber leading role in looking nearly adjusting is a circular fiber, the muscle fiber ring dwindles to the center as elastic ring during contraction, radius reduces, and dwindles and loose Suspensory ligament with the crystalline lens distance, shrinks strong more, point is sharp keen more in the ciliary processes, and the adjusting of generation is strong more; The stringer fiber may be led the effect that department connects the front and back stop, and owing to the preceding difference that goes up the some compliance makes the corpus ciliare displacement.Each performs its own functions for both, not significantly influence mutually, and this just provides foundation for comparative study organizational structure before observing myopia treatment back and treatment.

Four, " Chinese medicine ZIJING prescription " and ZIJING purification liquid one YANSUTAI ZHUSHEYE have been confirmed by above capsulociliary dynamic observing fully, medical new method is treated bathomorphic curative effect that histological foundation is provided " YANSUTAI ZHUSHEYE treatment myopia is annotated in a cave, eye district ", because ocular tissue's structure through treatment, muscular strength, the recovery of muscle group fiber impels the recovery of distant vision, the recovery of physiology of eye function, look far away, the recovery fully of looking nearly adjusting reaches leading organizational structure and functional level, therefore have clinically and cure the patient and still before treatment, live under the equal environment, study, myopia no longer takes place in eyes, and this just illustrates strengthens the purpose that the antifatigue effect reaches " holographic immunologic balance therapy " fully.Have among the patient of the recovery of clinical observation ocular tissue structure through 3 years to 13 years tests, have stalwartness, sensitive adjusting power is constant, all can make adjusting far away, near vision reach normal level, this assessment for myopia treatment back curative effect at a specified future date is significant.Modern with one part of the attached outpatient service statistical table in model case 32 example backs.

Description of drawings

Accompanying drawing 1 ocular anterior segment structure parameter measurement sketch map

The pleasing to the eye ciliary region UBM image in accompanying drawing 2 treatment backs

The specific embodiment

Embodiment 1

[purification medicinal liquid]

YANSUTAI ZHUSHEYE is a ZIJING purification active substance sterile water solution, and this product is colourless or little yellow supernatant liquid.For medicinal liquid is identified in the ZIJING representative.

The formulation method of YANSUTAI ZHUSHEYE and technological process

Method of purification: circulating reflux method of purification

Formulation method: Chinese medicine low capacity aqueous injection technological process

One, prescription: ZIJING 60g

Benzyl alcohol 2ml

3 of Tween 80s

Make injection 100ml

Two, operational approach:

Former medicine ZIJING is taken out, and removes the outsourcing Medulla Junci, washes, the 60g that weighs is put the former medicine of ZIJING and is twisted into the serosity shape in the reamer, adds injection water 100ml and soaks 1 hour, digestion is five times in water-bath, each digestion 30 minutes, after the filtration, slagging-off, filtrate concentrates and causes 1/2, in stirring, add 100 milliliters of 95% ethanol gradually, place, filtration, filtrate recycling ethanol.With 95% ethanol repeated treatments, causing does not have till the precipitation with method, and filtered and recycled ethanol adds benzyl alcohol, Tween 80, is adding 100ml water for injection, stir evenly, filtration, packing, sterilization, sample detection, bottle, seal, standby, intramuscular injection or acupoint injection therapy are used.

Every 1ml is equivalent to bright product ZIJING 1g or 2.5g, content: 1ml: 1g; 1ml: 2.5g; 2ml: 2g; 2ml: content among the every 1g of 5g testing result:

(1) trace element:

Selenium (Se): 0.020 μ g zinc (Zn): 0.047 μ g copper (Cu): 0.023 μ g

Chromium (Gr): 0.021g calcium (Ca): 9.20 μ g manganese (Mn): 0.042 μ g

Indicate: attached survey report China Metrology Accreditation (2002) amount is recognized (capital) word (Z0407)

(2) aminoacid

Threonine (Thr): 0.019mg glutamic acid (Gln): 0.243mg glycine (Gly): 0.101mg

Alanine (Ala): 0.098mg valine (Val): 0.039mg leucine (Len): 0.044mg

Phenylalanine (Phe): 0.027mg lysine (Lys): 0.030mg

Indicate: attached survey report China Metrology Accreditation (2002) amount is recognized (capital) word (Z0407)

(3) vitamin:

B1：0.14mg B2：0.20mg B12：0.0015mg

Indicate: attached survey report China Metrology Accreditation (2002) amount is recognized (capital) word (Z0407)

[medicine discriminating]

1. under the assay item, the aqueous solution of this product shows the discriminating of sodium salt and hydride but should meet " two appendix 3 of Chinese pharmacopoeia version in 2000.

2. the retention time of each aminoacid chromatographic peak should be consistent with the retention time of corresponding reference substance solution chromatographic peak in the chromatograph of noting down under the assay item.

The inspection technique of [medicine inspection] this product meets the " regulation of two appendix 1B of Chinese pharmacopoeia version in 2000.

1.PH value: 6.59 meet the " regulation of two appendix VIH of Chinese pharmacopoeia version in 2000.

2. protein: get this product 1ml and add 20% sulfo group water substance solution 1ml muddiness must not take place.

3. thermal source: get this product and check by the regulation of " two appendix XID of Chinese Pharmacopoeia version in 2000 ", dosage is up to specification by the every 1kg injection of rabbit 0.2ml.

4. bacterial endotoxin: the method " two appendix XIE of Chinese Pharmacopoeia version in 2000 " of pressing under the bacterial endotoxins test item is checked up to specification.

5. aseptic: as to check up to specification according to method under the sterility test method item " two appendix XIH of Chinese Pharmacopoeia version in 2000 ".

6. clarity: the regulation according to " clarity test detailed rules and regulations 80 criterions " is checked up to specification.

7. loading amount: carry out by " two appendix 1B of Chinese Pharmacopoeia version in 2000 " required standard.

Other: (1) solvent (2) additives (3) containers (4) ingredients (5) canned (6) sealings by fusing (7) loading amounts (8) leak detection (9) is stored.All up to specification by " two appendix 1B of Chinese Pharmacopoeia version in 2000 " required standard requirement.

[drug dose]: 1. ZIJING 5-30g

2. YANSUTAI 1ml: 1g; 1ml: 2.5g; 2ml: 2g; 2ml: 5g;

[scope of application]; All kinds of each phase myopia, developmental ophthalmocace, hypermetropia, astigmatism, stravismus, amblyopia, eyestrain, ocular injury, myopia complication, optic atrophy, non-suppurative keratitis, iridocyclitis, vitreous opacity, central retinochoroiditis, ZIJING are the prescription master of the myopia medicines of distinguishing the flavor of.

[clinical practice]: myopia is the disease based on deficiency syndrome, " deficiency syndrome should be treated by tonifying method ", the natural endowment deficiency, mend it day after tomorrow, deficiency of both the liver and kidney, asthenia of essence and blood, when suitable liver and kidney tonifying, beneficial essence is enriched blood and is method of treatment; Cross and use eyesight, overworked eyes causes declination of heart-YANG, or deficiency of liver-blood, controls suitable tranquilizing by nourishing the heart, or nourishing blood to suppress the hyperactive liver; Eating and drinking without temperance, impairing the spleen and stomach, insufficiency of vital energy and blood is controlled suitable invigorating the spleen and benefiting QI; A little less than the deficiency of vital energy blood, blood fortune is unable, or qi depression to blood stasis, controls suitable benefiting vital QI and blood, blood circulation promoting and blood stasis dispelling.In, the low myopia eye, often based on deficient syndrome, rule of treatment method of treatment then should be based on tonify deficiency; High myopic eye is this with void, but deteriorated case is numerous and disorderly, and deficiency complicated with excess is often arranged.Or show as principal contradiction with excess syndrome, by the principle of the traditional Chinese medical science " symptomatic treatment in acute condition ", " radical treatment in chronic case ", this moment should be based on timid heresy.As cooling blood for hemostasis, clearing away heat-damp and promoting diuresis, method of treatment such as blood circulation promoting and blood stasis dispelling.Certainly, when timid heresy, should expect deficiency in origin.Because of this cause of disease because of complexity, disease is various, should be principle with " symptomatic treatment in acute condition, relieving the primary symptom in a chronic case " at ordinary times, the body resistance strengthening and constitution consolidating, liver and kidney tonifying, invigorating the spleen and benefiting QI is main, simultaneously, also be the treatment this disease therapy commonly used.

Embodiment 2 deficiency of heart-YANG types:

[clinical manifestation]: myopia is clear, distant vision being blurred, or companion's insomnia forgetfulness, spiritlessness and weakness, pale tongue with thin fur, weak pulse.

[pathogenesis analysis]: fire is refreshing light at order, crosses and uses eyesight, overworked eyes, it is on the decline to cause heart-yang, and excess of YIN due to YANG-insufficiency, refreshing light are difficult to send out more at a distance, so look closely clear, distant vision being blurred, blood must not be gone up honor, then the complexion white light, the main hypermetropia of dispersing of sun, cloudy main convergence is looked near, and deficiency of heart-YANG then yang-energy can not be dispersed, and causes distant vision being blurred; The moon is had a surplus then can restrain and is looked closely.The heart controlling mental and emotional activities if lose yang-energy, agitates unable, blood vessels not fill, refreshing light must not, send out more at a distance, thus look near timid far.Then spirit, consciousness and thinking activities weaken, and insomnia forgetfulness very then occurs, and be refreshing tired weak, pale tongue with thin fur, and weak pulse is the disease of deficiency of heart-YANG.

[rule of treatment]: reinforcing the heart QI invigorating, tranquilizing the mind

[square medicine]: (Ziqing tranquilizing drug)

[prescription]: ZIJING 30g, Medulla Junci 30g, Radix Polygalae 20g, Radix Codonopsis 5g, Poria cum Radix Pini 15g, Concha Margaritifera 15g, Rhizoma Acori Graminei 10g, Cortex Cinnamomi 15g, Radix Astragali 10g, Radix Angelicae Sinensis 10g, Fructus Schisandrae Chinensis 10g, Fructus Leonuri 10g, Fructus Lycii 10g, Semen Ziziphi Spinosae 10g, Semen Plantaginis 10g, Semen Cuscutae 10g ， Zhu sand 3g, Radix Glycyrrhizae 5g.

[plus-minus]: yang deficiency person adds Cortex Cinnamomi, the QI invigorating warming YANG; The heart-throb person adds Semen Platycladi to nourish heart.Dosing is fried in shallow oil and is introduction and the same clothes of ball.

[method for making]: process is processed into ball, the heavy 1g of ball, every bag net weight 12g, and taking medicine before meal usefulness, each bag, every day three times, two weeks were a course of treatment.

Be equipped with injection: YANSUTAI ZHUSHEYE 2ml * 2, once-a-day, intramuscular injection.

Embodiment 3 insufficiency of spleen-qi type:

[clinical manifestation]: being clear, distant vision being blurred, being unable to see for a long time, happiness is hung down and is closed, or weak after being ill, inappetence, limbs fatigue, pale tongue with thin fur is white, weak pulse.

[pathogenesis analysis]: because monophagia, malnutrition or excessive causes spleen injury, deficiency-weakness of spleen-QI.The spleen has the function to transport and transform nutrients, main the lucid yang sending up, insufficiency of the spleen then cereal nutrient can not on fail in purpose, cause malnutrition of eyes, then distant vision being blurred.Insufficiency of the spleen deficiency of QI in middle-JIAO, the unable then being unable to see for a long time of elevate a turnable ladder, happiness is hung down and is closed.Insufficiency of the spleen failure of the spleen to transport and transform, retention of water-damp in the body causes the whole body insufficiency of vital energy and blood, then limbs fatigue.Pale tongue with thin fur is white, and weak pulse is the disease of deficiency-weakness of spleen-QI.

[rule of treatment]: invigorating the spleen and benefiting QI

[square medicine]: (Zijing spleen tonifying pill)

[prescription]: ZIJING 30g, Radix Codonopsis 20g, Rhizoma Atractylodis Macrocephalae 10g obtains Siberian cocklebur 15g, Radix Polygoni Multiflori 20g, Radix Paeoniae Alba 20g, Pericarpium Citri Reticulatae 10g, Rhizoma Atractylodis Macrocephalae 10g, Rhizoma Dioscoreae 10g, Radix Platycodonis 10g, Rhizoma Dioscoreae 10g, Semen Coicis 10g, Radix Puerariae 10g, Fructus Leonuri 10g, Fructus Lycii 10g, Semen Cassiae 10g, Flos Chrysanthemi 10g, Radix Glycyrrhizae Preparata 5g.

[plus-minus]: the inappetence person of laying particular stress on, add Massa Medicata Fermentata, Fructus Crataegi, digestion-promoting spleen-invigorating, insufficiency of vital energy and blood is the person partially very, adds Radix Angelicae Sinensis, the Radix Astragali, vigorate qi and replenish the blood.

[method for making]: through being processed into ball, the heavy 1g of ball, every bag net weight 12g, taking medicine before meal usefulness, each bag, every day three times.

The deficient type of embodiment 4 kidney yins:

[clinical manifestation]: labor is looked forward or upwards watching for a long time, visual deterioration, caligation, mouthful in desire drink, stomach receive still can, the stool profit, black flower dances in the air, eyelash is unable, order is closed in happiness, dizziness, tinnitus, forgetful, insomnia, the soreness of waist is soft.

[pathogenesis analysis]: waist is the kidney mansion, suffers from a deficiency of the kidney and can not make by force then soreness of the waist and knees.Lower limb is the medulla renis sea, deficiency of marrow-reservoir, suffer from a deficiency of the kidney can not on hold then, look near timid far away, dizziness and tinnitus, insomnia forgetfulness, the red few tongue of tongue trade, deep-thready pulse is the deficient disease of kidney yin.

[rule of treatment]: tonification is suffered from a deficiency of the kidney, and having one's ideas straightened out makes eye bright.

[square medicine]: (Zijing kidney reinforcing pill)

ZIJING 30g, Radix Rehmanniae Preparata 20g, Fructus Corni 15g, Rhizoma Dioscoreae 15g, Poria 15g, Rhizoma Alismatis 15g, Cortex Moutan 10g, Herba Dendrobii 20g, Radix Salviae Miltiorrhizae 15g, Radix Curcumae 10g, Fructus Amomi 10g, Fructus Broussonetiae 10g, Cordyceps 20g, Fructus Lycii 15g, Fructus Schisandrae Chinensis 15g, Semen Plantaginis 10g, Semen Cassiae 10g, Herba Senecionis Scandentis 10g.

[plus-minus]: insufficiency of kidney-YANG adds Ramulus Cinnamomi, Radix Aconiti Lateralis Preparata, and the heating of suffering from a deficiency of the kidney adds Radix Bupleuri, and the dryness of the intestine constipation adds Radix Angelicae Sinensis, Radix Et Rhizoma Rhei.

[method for making] grinds to the powder altogether, and banburying is a ball, the heavy 15g of ball, one after each meal sooner or later.

Embodiment 5 deficiency of liver-blood types:

[clinical manifestation]: look closely still clear, distant vision being blurred, being unable to see for a long time, dim-sighted at the moment, looking one is two, metamorphopsia, black Hua Jiansheng, examination of ocular fundus, visible macula lutea portion maculae atrophicae or hemorrhage, complexion is very white, the colour of skin is not magnificent, myasthenia of limbs, pale tongue with thin fur is white, weak pulse.

[pathogenesis analysis]: polyphyly is crossed and is used eyesight, and labor is looked forward or upwards hypermetropia, consumption impairment of YIN blood, being unable to see for a long time.The irritability store blood, the liver blood rareness, not nourishing the liver of blood, liver opening at eye, liver can be looked by blood, and deficiency of liver-blood is loss of nutrient of eyes then, looks thing ghost image, fuzzy, looks dizziness of a specified duration, dizzy, metamorphopsia.Deficiency of liver-blood can not be gone up flourish in women's head-ornaments, so face is very white, the colour of skin is simple and unadorned, myasthenia of limbs, and pale tongue with thin fur is white, and weak pulse is the disease of deficiency of liver-blood.

[rule of treatment]: liver-smoothing, qi-regulating, nourishing liver blood.

[square medicine]: (Zijing liver tonifying soup)

[prescription]: ZIJING 30g, Radix Angelicae Sinensis 30g, Radix Paeoniae Alba 15g, Rhizoma Chuanxiong 15g, Radix Rehmanniae Preparata 15g, Semen Sesami Nigrum 15g Radix Ophiopogonis 15g, Fructus Chaenomelis 15g, Semen Ziziphi Spinosae 10g, Fructus Lycii 15g, Flos Chrysanthemi 15g, Radix Saposhnikoviae 15g, Radix Asparagi 15g, RHIIZOMA DIOSCOREAE frOm Henan of China 15g, Poria 15g, Semen Plantaginis 30g, Radix Platycodonis 12g, Radix Bupleuri 12g, Herba Dendrobii 15g, Herba Asari 3g, Fructus Schisandrae Chinensis 12g, Radix Polygalae 12g, Semen Ziziphi Spinosae (parched) 15g, Radix Glycyrrhizae 3g.

[plus-minus]: add the Radix Astragali, Radix Codonopsis with vigorate qi and replenish the blood.

[method for making]: be decocted in water for oral dose, one day twice, the morning and evening one after each meal.

Embodiment 6 liver energy stagnation type of suffering from a deficiency of the kidney:

[clinical manifestation]: will be very fast under the vision, teenager is suffered from more.Looking thing can not watching for a long time, overworked eyes, and dizzy ophthalmic bloated, anorexia, perspiration lacks, xerostomia desire drink, forehead is overcautious, and eyelash is unable, does not desire to open eyes, and happiness is closed, sour, dry and astringent, the proptosis ocular of eye, and black flower waves, and after the lightning, looks as dim the close knot of defecating, weakness of the waist and knees

[pathogenesis analysis]: refreshing light is at order, and gas decreases dispirited, deficiency of kidney yin, the liver blood loss, in have hot and suffocating, a little less than the deficiency of the kidney yin, stagnated heat of liver channel, body of the tongue is light red, tongue is thin white, deep and stringy pulse is counted accurately or is heavy and unable.

[rule of treatment]: nourishing YIN and benefiting blood, liver heat removing and order

[square medicine]: (Zijing pill for reinforcing kidney and soothing liver)

[prescription]: ZIJING 30g, ripe Radix Rehmanniae 30g, Fructus Lycii 12g, Radix Codonopsis 20g, Radix Paeoniae Alba 15g, Semen Astragali Complanati 10g, Fructus Mori 10g, Fructus Ligustri Lucidi 10g, Radix Adenophorae (Radix Glehniae) 10g, Radix Scutellariae 10g, Rhizoma Pinelliae 10g, Radix Bupleuri 10g, Herba Schizonepetae 10g, Fructus Leonuri 10g, Semen Plantaginis 10g, Radix Salviae Miltiorrhizae 10, Rhizoma Chuanxiong 10g, Medulla Junci 10g, Semen Cassiae 10g, Radix Saposhnikoviae 10g, Rhizoma Cyperi 10g, Radix Angelicae Sinensis 5g, Spica Prunellae 15g, Herba Epimedii 10g, Radix Polygoni Multiflori Preparata 10g, Fructus Corni 10g, Cordyceps 15g, Radix Glycyrrhizae 3g.

[plus-minus]: the close knot of defecating adds fence and rushes down leaf, Radix Ophiopogonis.The dysuria person adds Poria, Polyporus.

[method for making] grinds to the powder altogether, and banburying is a ball, and the heavy 15g of ball respectively obeys 1 ball sooner or later.

Embodiment 7 caused by liver and kidney deficiency types:

[clinical manifestation]: distant vision descends, the pallor giddy, black at the moment flower dances in the air, whole body disease as seen, dizziness and tinnitus, insomnia and dreamful sleep, soreness of the waist and knees, pale tongue without fur, thready pulse.

[pathogenesis analysis]: morbidity since the childhood, polyphyly natural endowment deficiency, caused by liver and kidney deficiency, few through blood deficiency, refreshing light is slight, brilliance can not cure and, can not sending nutrient upward to eye, thus sincerely can myopia, eyes losing nutrition, then black at the moment spending danced in the air.Brain being the marrow sea, suffer from a deficiency of the kidney can not on hold, deficiency of marrow-reservoir, dizziness and tinnitus then, waist is a house of kidney, suffers from a deficiency of the kidney that it is strong to do, soreness of the waist and knees then, the light red tongue thready pulse is the disease of caused by liver and kidney deficiency.

[rule of treatment]: liver and kidney tonifying

[square medicine]: (Zijing liver tonifying pill)

[prescription]: ZIJING 30g, Radix Codonopsis 15g, Radix Rehmanniae 15g, Radix Angelicae Sinensis 15g, Concha Haliotidis 15g, Concha Margaritifera 15g, Fructus Corni 10g, Rhizoma Dioscoreae 10g, Radix Stephaniae Tetrandrae 10g, Rhizoma Alismatis 10g, Flos Chrysanthemi 10g, Radix Salviae Miltiorrhizae 10g, Fructus Lycii 10g, Ramulus Cinnamomi 10g, Radix Aconiti Lateralis Preparata 10g, Fructus Broussonetiae 10g, Fructus Mori 10g, Fructus Ligustri Lucidi 10g, Fructus Leonuri 10g, Semen Plantaginis 10g, Rhizoma Chuanxiong 10g, Fructus Schisandrae Chinensis 10g, Os Draconis 10g, Radix Paeoniae Alba 10g, Semen Cassiae 10g, Fructus Amomi, Rhizoma Acori Graminei, Radix Polygalae, Radix Saposhnikoviae, Faeces Vespertilionis, Herba Senecionis Scandentis, Poria cum Radix Pini, the yellow bat, Radix Aucklandiae (Radix Vladimiriae), each 8g of JIXUEXI, Radix Glycyrrhizae 5g.

[method for making] be porphyrize altogether, and banburying becomes ball, the heavy 10g of ball, twice of every day.

[adapted] YANSUTAI ZHUSHEYE 2ml every day * per two weeks of 2 intramuscular injection are a course of treatment.

Embodiment 8 taste deficiencies:

[clinical manifestation]: distant vision descends, and near vision is normal, blurring of vision, and watching for a long time is weak, and binocular is closed in happiness, and the face light yellow complexion is simple and unadorned, the sight dimness, muscle is thin, and body is tired unable, monophagia and anorexia, the wrist abdomen goes up painful, and light red tongue is gargled also the indentation white and thin fur, a little less than the moderate pulse.

[pathogenesis analysis]: The spleen and stomach provide the material basis of the acquired, a little less than upright digesting and absorbing the essence of foodstuff far away and the defeated cloth essence, is the source of QI and blood elder generationization, and weakness of the spleen and stomach QI and blood elder generationization is not enough, is difficult to support order key, the muscle of spleen; Labor is looked forward or upwards watching for a long time, and muscle weakness loses balance, outer flesh stravismus, interior muscular spasm, it is unable to lift eyelash, can not hypermetropia, look also clear, insufficiency of the spleen transformation failure of spleen, promoting circulation of body fluid not, retention of water-damp, the QI and blood source is not enough, so color be that Huang is simple and unadorned, refreshing light dimness, wasting, body is tired unable.Failure of the spleen to transport and transform, indigestion, so clear QI being mixed up with turbid QI is the loose stool.Hypoactivity of spleen-YANG, so dysfunction of the spleen in transportation and transformation is food back distension and fullness in the abdomen.There is indentation on the light red tongue limit of coughing, white and thin fur, the resembling of weak and taste two void of moderate pulse.

[rule of treatment]: invigorating the spleen and regulating the stomach, replenishing vital essence to improve eyesight

[square medicine]: (Zijing stomach tonifying pill)

[prescription]: ZIJING 30g Radix Astragali 15g Radix Codonopsis 15g Radix Angelicae Sinensis 15g Pericarpium Citri Reticulatae 10g Endothelium Corneum Gigeriae Galli (vinegar moxibustion) Rhizoma Dioscoreae Fructus Amomi

Radix Platycodonis Semen Nelumbinis Radix Aucklandiae Semen Coicis Rhizoma Cimicifugae Radix Bupleuri Rhizoma Atractylodis Macrocephalae Fructus Crataegi Fructus Setariae Germinatus Semen Ziziphi Spinosae Poria

Radix Paeoniae Alba Radix Polygalae Medulla Junci Cortex Cinnamomi Massa Medicata Fermentata Radix Rehmanniae Preparata Fructus Lycii red sage and chuanxiong rhizome Semen Cuscutae Semen Astragali Complanati

Fructus Mori Fructus Ligustri Lucidi Semen Cassiae Fructus Leonuri, each 10g Radix Glycyrrhizae 5g of Fructus Broussonetiae Semen Plantaginis Fructus Jujubae.

[method for making] is through being processed into pillet, the every bag net weight of the heavy 1g of ball 12g

Each bag, day clothes three times, taking medicine before meal is used.

Be equipped with injection: YANSUTAI ZHUSHEYE, inject 2ml * 2 every day, per two weeks of intramuscular injection are a course of treatment.

Embodiment 9 liver spleen deficiencies:

[clinical manifestation]: being clear, distant vision being blurred, the sight dimness, slow-witted order does not have god, inappetence, anorexia, monophagia, few food, emaciation and sallow complexion, myasthenia of limbs, weak after being ill, pale tongue with thin fur is white, thready and weak pulse.

[etiology and pathogenesis]: The spleen has the function to transport and transform nutrients, main the lucid yang sending up, insufficiency of the spleen then precise and tiny can not fail in liver, liver do not conserve, that irritability is on the decline, liver is insufficiency of the spleen is weak, deficiency of QI in middle-JIAO, retention of water-damp in the body, insufficiency of vital energy and blood.Asthenia of liver and spleen is an asthenia of essence and blood, and two orders lose supports.Refreshing light is loved little, and refreshing light is for the source deficiency, and brilliance can not reach with at a distance, so myopia is timid far away, axis oculi elongates, and it is prominent that eyeball becomes, the anterior chamber deepens, mydriasis, and light reflex is blunt, essence and blood can not be gone up honor, YANG-orifices failing to be nourished, the dreaminess so dizzy, dizzy, tinnitus, night are slept, asthenia of essence and blood, sering is not filled, thready pulse without strength then, the few tongue of red tongue.

[rule of treatment]: the block removing of liver and kidney tonifying benefit intensive culture blood suppressing the hyperactive liver to relieve the wind syndrome makes eye bright

[square medicine]: (nebula removing pills)

[prescription]: each each 10g Radix Glycyrrhizae 5g Herba Asari 3g of 15g Os Draconis Endoconcha Sepiae Herba Cistanches Radix Rehmanniae Preparata Flos Chrysanthemi medicines rehmannia rhizome, cornus officinalis Radix Polygoni Multiflori Preparata Radix Bupleuri white peony root Radix Asparagi Radix Ophiopogonis Rhizoma Acori Graminei Radix Polygalae Radix Saposhnikoviae Faeces Vespertilionis Cortex Moutan Rhizoma Alismatis Poria cum Radix Pini Radix Astragali Radix Codonopsis Radix Angelicae Sinensis Rhizoma Chuanxiong Rhizoma Anemarrhenae Cortex Phellodendri Radix Aucklandiae (Radix Vladimiriae) Herba Dendrobii Caulis Spatholobi of ZIJING 30g Concha Margaritifera 30g Concha Haliotidis 20g Fructus Lycii Fructus Leonuri Semen Plantaginis Semen Cuscutae Fructus Rosae Laevigatae Fructus Mori Fructus Rubi Fructus Alpiniae Oxyphyllae Fructus Schisandrae Chinensis

[method for making] grinds to the powder altogether, and banburying is a ball, the heavy 20g of ball, and day twice, one time one ball of clothes, one after each meal, eliminating cold for resuscitation water send down.

15 days one courses of treatment of YANSUTAI ZHUSHEYE 1ml * 0.6 * 2 district's injection are annotated in a cave in [adapted] (1) eye district

(2) eye muscle training

Embodiment 10 ZIJING FUMING YANTIE:

[clinical practice]: all can not be oral and the myopia and the near-sighted deteriorated case of injection, each phase, various patient all can use, but must instruct application by the doctor.

[rule of treatment]: blood circulation promoting and blood stasis dispelling, dredge the meridian passage, liver and kidney tonifying, nourishing QI and blood, suppressing the hyperactive liver to relieve the wind syndrome, liver heat removing and eyesight improving.

[prescription]: ZIJING 80g Calamina 50g, Margarita 30g, Moschus 1g, Fel Ursi 1g, Borneolum Syntheticum 1g, Borax 3g, Os Sepiae 30g, Radix Angelicae Dahuricae 30g, Fructus Viticis 15g, Fructus Lycii 15g, Semen Cuscutae 15g, Semen Cassiae 15g, Fructus Leonuri 15g, Fructus Tribuli 12g, Periostracum Cicadae 9g, Endoconcha Sepiae 12g, Olibanum 10g, Myrrha 10g, Semen Coicis 9g, Flos Carthami 9g, Rhizoma Chuanxiong 10g.

[method for making]: should echo regulation according to " Chinese Pharmacopoeia two appendix IV in 2000 ".

Calamina, forge porphyrize, with the Borneolum Syntheticum mix homogeneously, divide and do two parts, one splits in the porcelain, fries in shallow oil water logging with following prescription, use the cotton paper sealing on the porcelain mouth, weather exposure adds Margarita powder 30g when dried, Fel Ursi 1g, Moschus 1g, Borax 3g, lapping-in is even altogether to be dipped in Calamina together with medication not, makes eye and pastes.

It more than is the announcement that nearly timid 24 diseases, 8 types far away of energy are secretly handed down prescription.About near-sighted deteriorated case: cloud and mist moves fine, considering the straight line as the curve due to metamorphopsia, and sudden blindness 72 symptoms " close biography 58 prescriptions of purpurine " treat that arrangement delivers after the demonstration again.

The basic research and the applied research brief summary of ZIJING treatment myopia: " opinion is achieved unusual years, looks into the future and shoulders heavy responsibilities ".I am the traditional Chinese medical science, 48 years achievements of practising medicine, and 43 years scientific research career is realized me deeply: " student's oculopathy is not minor matter, especially a myopia." according to epidemiology statistics, constitutional myopia 81.4% occurs among the student, has only 18.6% to flow in social crowd, it seems that the student is the ill source of myopia.In state-owned 1,300,000,000 populations, the student accounts for 400,000,000, and has 300,000,000 to be myopia among the student 400,000,000.Serious is 2002 according to the Ministry of Education of the state and health ministry investigation monitoring and statistics, because of 300,000 people that have of myopia blinding, how surprising this be numeral.We investigate through a large amount of retrieval investigation: myopia has certificate at 49 country's reports, spread all over different continents, so myopia is global human difficult and complicated illness.Myopia is the oculopathy with inherited characteristic, though frequency is less, indication will be that human visual deterioration becomes bathomorphic tendency, otherwise several centuries, human blinding, the frequency that disables constantly increases, and is the threat of a maximum to the human development future, and control ahead of time stops tragedy to take place.

Claims (3)

1, the application of Chinese medicine ZIJING in the preparation of preparation treatment ophthalmic diseases, wherein ZIJING is meant the healthy whole eyeball of the healthy cattle whole eyeball in vertebrates Bovidae Ziyang or horse, hinny.

2, application as claimed in claim 1, wherein the place of production of Ziyang cattle is Ziyang, Shaanxi, Shitai County, Anhui or enshi.

3, application as claimed in claim 1 or 2, wherein the preparation method of ZIJING is: when slaughtering an ox fresh and alive eyeball is extracted the flush away bloodstain, remove clean lipid foreign material on every side, wrap up, dry in the shade with Medulla Junci behind the salting, the freezing antistaling storing is avoided with fiery baking, Exposure to Sunlight, wind in case break rotten.

Images