CN1326883C - Method for preparing calcium hyauronate - Google Patents
Method for preparing calcium hyauronate Download PDFInfo
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- CN1326883C CN1326883C CNB2005101173009A CN200510117300A CN1326883C CN 1326883 C CN1326883 C CN 1326883C CN B2005101173009 A CNB2005101173009 A CN B2005101173009A CN 200510117300 A CN200510117300 A CN 200510117300A CN 1326883 C CN1326883 C CN 1326883C
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- calcium
- hyaluronate
- alcoholic solution
- calcium salt
- preparation
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Abstract
The present invention provides a method for preparing calcium hyaluronate which is hyaluronic calcium salt. The method has the technology that sodium hyaluronate solid bodies in alcoholic solution are directly turned to the calcium hyauronate with calcium salt by an ion exchange method. The present invention has the advantages of simple and direct production technology, few devices and low cost.
Description
Technical field
The invention belongs to field of health care food, relate to a kind of preparation method of Calcium hyaluronate.
Background technology
Calcium hyaluronate is hyaluronic calcium salt, the polysaccharide that hyaluronic acid (hyaluronic acid) is made up of the disaccharide repeating unit of glucuronic acid and glucosamine, be present in many reticular tissue such as skin, vitreum, umbilical cord, cartilage, knuckle synovia, and play therein preserve moisture, physiological actions such as nutrition, reparation and pre-antisitic defect.Calcium hyaluronate can be used as capsule or solution, and hyaluronic acid has good cosmetic result, and Calcium hyaluronate is again good calsium supplement, therefore as protective foods, not only has cosmetic result but also have the good health care effect.
Traditional preparation technology is a spent ion exchange resin, and with the hyaluronate sodium dissolving, upper prop changes into Calcium hyaluronate by ion-exchange with hyaluronate sodium, again by organic solvent deposit, and dehydration, vacuum-drying.This method yield is low, and the production cycle is long, complex procedures, and use equipment is many, and the present invention only finishes at dehydrating step, and the yield height, use equipment is few, and technology is simple and easy to do.
Summary of the invention
The invention provides a kind of preparation method of Calcium hyaluronate, be with the hyaluronate sodium pressed powder in the alcoholic solution of calcium salt by the method for ion-exchange, directly change into Calcium hyaluronate.
Organic solvent of the present invention comprises methyl alcohol and ethanol.
Calcium salt of the present invention, for calcium chloride, calcium sulfate, lime acetate, nitrocalcite etc. can be dissolved in the inorganic or organic zinc salt of small molecules of alcohol-water solution, wide material sources are easy to get.
Its concentration of alcohol-water solution of the present invention is 40%～100% (volume fraction)
This invention is solid powdery product, calcium contents 3%～12%.
The preparation technology of Calcium hyaluronate is in this invention: A. is soaked into hyaluronate sodium in the alcoholic solution that contains calcium salt, stirs to be beneficial to the speeding-up ion exchange, filters, and removes alcoholic solution.B. repeat A step 2～10 time, obtain the wet solid of Calcium hyaluronate.C. soak the wet solid of washing Calcium hyaluronate with the alcoholic solution that does not contain calcium salt.D. the dry Calcium hyaluronate that gets.
The specific embodiment of the invention:
Following examples are for illustrating in greater detail the present invention, are not restriction the present invention.
Embodiment 1
With hyaluronate sodium 180 grams, stir, add 2 liters of ethanolic solns (alcohol concn 92%) that contain 1.5% calcium sulfate, open stirring, after 12 hours, stop to stir, the alcohol supernatant liquor inclines, repeat displacement step 8 times, use 95% washing with alcohol afterwards 5 times, to alcoholic strength 95%, centrifugal, remove ethanol, put the vacuum drying oven drying, to moisture 7%.
Embodiment 2
With hyaluronate sodium 230 grams, stir, add 2.5 liters of methyl alcohol (methanol concentration 90%) that contain 3% calcium chloride, open stirring, after 12 hours, stop to stir, the methyl alcohol supernatant liquor inclines, adding 2 liters of methyl alcohol that contain calcium chloride again repeats to replace 8 times, use the analytical pure methanol dehydration afterwards 5 times, centrifugal, remove methyl alcohol, put the vacuum drying oven drying, to moisture 7%.
Embodiment 3
With hyaluronate sodium 200 grams, stir, add 2 liters of ethanol (alcohol concn 90%) that contain 3% lime acetate, open stirring, after 12 hours, stop to stir the ethanol that inclines supernatant liquor, adding 2 liters of ethanol that contain lime acetate again repeats to replace 8 times, use 90% ethanol dehydration 5 times afterwards, centrifugal, remove ethanol, put the vacuum drying oven drying, to moisture 7%.
Claims (6)
- The preparation method of 1 one kinds of Calcium hyaluronates, it is characterized in that with the hyaluronate sodium pressed powder in the alcoholic solution of calcium salt by the method for ion-exchange, directly change into Calcium hyaluronate.
- The described preparation method of 2 claims 1 is characterized in that described calcium salt is for being dissolved in the inorganic or organic calcium salt of small molecules of alcoholic solution.
- The described preparation method of 3 claims 2 is characterized in that the inorganic or organic calcium salt of the described small molecules that can be dissolved in alcoholic solution is calcium chloride, calcium sulfate, lime acetate or nitrocalcite.
- The described preparation method of 4 claims 2 is characterized in that the concentration of described calcium salt in alcoholic solution is 1%～15%.
- The arbitrary described preparation method of 5 claim 1-4 is characterized in that described alcoholic solution is the methyl alcohol or the alcoholic acid aqueous solution, and its liquor capacity mark is 40%～100%.
- The described preparation method of 6 claims 1 is characterized in that Calcium hyaluronate is the solid powdery product, calcium contents 3%～12%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101173009A CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101173009A CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1748569A CN1748569A (en) | 2006-03-22 |
| CN1326883C true CN1326883C (en) | 2007-07-18 |
Family
ID=36604370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101173009A Active CN1326883C (en) | 2005-11-04 | 2005-11-04 | Method for preparing calcium hyauronate |
Country Status (1)
| Country | Link |
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| CN (1) | CN1326883C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831004A (en) * | 2010-03-24 | 2010-09-15 | 章云 | Hyaluronic acid strontium salt compounds, preparation method thereof and application in medicine |
| EP2559708B1 (en) * | 2010-04-14 | 2018-06-20 | Kewpie Corporation | Process for producing zinc hyaluronate, process for producing cosmetic containing zinc hyaluronate, zinc hyaluronate and process for producing the same |
| WO2023125789A1 (en) * | 2021-12-31 | 2023-07-06 | 华熙生物科技股份有限公司 | Calcium hyaluronate, and preparation method therefor and use thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0622560B2 (en) * | 1989-04-11 | 1994-03-30 | 株式会社紀文食品 | Method for producing hyaluronate sheet |
| CN1537868A (en) * | 2003-04-18 | 2004-10-20 | 上海建华精细生物制品有限公司 | Preparation method of sodium hyaluronic acid |
| CN1563108A (en) * | 2004-04-13 | 2005-01-12 | 阮春学 | Method for preparing transparent calcium hyaIuronate in low molecular weight |
| CN1674920A (en) * | 2002-08-07 | 2005-09-28 | 梅迪多姆实验室股份有限公司 | Process for preparing a sterile high molecular weight hyaluronic acid formulation |
-
2005
- 2005-11-04 CN CNB2005101173009A patent/CN1326883C/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0622560B2 (en) * | 1989-04-11 | 1994-03-30 | 株式会社紀文食品 | Method for producing hyaluronate sheet |
| CN1674920A (en) * | 2002-08-07 | 2005-09-28 | 梅迪多姆实验室股份有限公司 | Process for preparing a sterile high molecular weight hyaluronic acid formulation |
| CN1537868A (en) * | 2003-04-18 | 2004-10-20 | 上海建华精细生物制品有限公司 | Preparation method of sodium hyaluronic acid |
| CN1563108A (en) * | 2004-04-13 | 2005-01-12 | 阮春学 | Method for preparing transparent calcium hyaIuronate in low molecular weight |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1748569A (en) | 2006-03-22 |
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Owner name: SHANDONG FREDA BIOPHARM CO., LTD. Free format text: FORMER NAME: FURUIDA BIOCHEMICAL CO., LTD., SHANDONG |
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Address after: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee after: Shandong Freda Biopharm Co., Ltd. Address before: 250014 No. 264 mountain road, Lixia District, Shandong, Ji'nan Patentee before: Furuida Biological Chemical Co., Ltd., Shandong |
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Address after: 250101 678 Tianchen street, hi tech Development Zone, Ji'nan, Shandong Patentee after: Huaxi Biotechnology Co., Ltd. Address before: 250101 678 Tianchen street, hi tech Development Zone, Ji'nan, Shandong Patentee before: Bloomage Freda Biopharm Co., Ltd. |
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