CN1324659A - Vagina spry containing biological active factor - Google Patents
Vagina spry containing biological active factor Download PDFInfo
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- CN1324659A CN1324659A CN 00114296 CN00114296A CN1324659A CN 1324659 A CN1324659 A CN 1324659A CN 00114296 CN00114296 CN 00114296 CN 00114296 A CN00114296 A CN 00114296A CN 1324659 A CN1324659 A CN 1324659A
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- vagina
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- interferon
- fibroblast growth
- vagina spray
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Abstract
The present invention discloses a vaginal spray containing bio-active factor. It contains 1-100 microgran/ml alkaline fibroblast growth factor, interferon and extract of Chinese medicinal materials of enidium seed, phellodendron bark, flavescent sophora root, stemona root and coptis root. Said spray not only has the bactericidal and inflammation-resisting action, but also possesses the functions of regulating metabolic activity of vaginal epithelial cell, raising its immunological activity and strong promoting action for repairing cervical, vaginal and vulval damage.
Description
The present invention relates to a kind of vagina spray that contains bioactie agent.
The Chinese medicine of effects such as the vagina spray of existing treatment gynecological infection is antibiotic by having usually, heat clearing away, parasite killing is formed, as disclosed vagina spray among the Chinese patent CN1114566.These vagina sprays have antiinflammatory, dehumidifying, effect such as antipruritic, but to the damage at positions such as pudendum, vaginal mucosa, cervix uteri, and are powerless as the active reparation of the local damage that caused after inflammation and laser, the physiotherapy such as freezing and ulcer.
The object of the present invention is to provide a kind of vagina spray that pudendum, vaginal mucosa and cervix uteri damage is had its active repair of promotion.
Vagina spray of the present invention, contain 1～100 μ g/ml basic fibroblast growth factor, 10～1,000,000 u/ml α 2a type interferon or α 1b type interferon, also can contain Chinese medicine extract, 0.1～0.5% hyaluronic acid and 0.05%～0.1% Mentholums such as Fructus Cnidii, Cortex Phellodendri, Radix Sophorae Flavescentis, the Radix Stemonae, Rhizoma Coptidis in addition.
The method for preparing vagina spray of the present invention, be made up of following steps: (1) is with basic fibroblast growth factor, interferon and/or albumin and/or mannitol and/or the chitin polysaccharide is independent or dissolve with water for injection the mixing back, filtration, degerming then, lyophilized powder is made in last lyophilization; (2) Chinese medicines such as Fructus Cnidii, Cortex Phellodendri, Radix Sophorae Flavescentis, the Radix Stemonae, Rhizoma Coptidis, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.05%～0.1% Mentholum, 0.1～0.5% hyaluronic acid is standby.(3) face the time spent, lyophilized powder is poured in the made Chinese medicine extract solution, mixing can use.
Basic fibroblast growth factor is the active substance of denier in the human body, has induction of vascular generation, microcirculation improvement, promotion fibroblast, epithelial cell, sarcoplast, neurocyte and physiological actions such as osteocyte growth and reparation.Therefore vagina spray of the present invention not only has the antiphlogistic effect of sterilization, but also has the effect of regulating the vaginal epithelial cell metabolic activity, strengthening its immunocompetence, prevent pudendum infection and ulcer, promotion vaginal injury initiatively to repair.Vagina spray of the present invention is applicable to the reparation of external genital that multiple factor causes, vagina, cervical mucous membrane damage and the prevention and the treatment of bacillary, fungoid, trichomonal vaginitis and sexually transmitted disease (STD).
Embodiment 1
(1) 10 μ g basic fibroblast growth factors are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved in the Chinese medicine extract uses.
Embodiment 2
(1) 50 μ g basic fibroblast growth factors and 0.05g mannitol are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved among the Chinese medicine extract 20ml uses.
Embodiment 3
(1) 10 μ g basic fibroblast growth factors and 0.1g albumin are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved among the Chinese medicine extract 20ml uses.
Embodiment 4
(1) 5 μ g basic fibroblast growth factors, 50 μ g α 2a type interferon, 0.1g albumin, 0.05g mannitol are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved among the Chinese medicine extract 20ml uses.
Embodiment 5
(1) 15 μ g basic fibroblast growth factors, 50 μ g α 2a type interferon, 0.05g mannitol are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved among the Chinese medicine extract 20ml uses.
Embodiment 6
(1) 10 μ g basic fibroblast growth factors, 50 μ g α 1b type interferon, 0.05g mannitol are dissolved with 10ml water for injection, filter then, degerming, lyophilized powder is made in last lyophilization; (2) Fructus Cnidii 30g, Cortex Phellodendri 30g, Radix Sophorae Flavescentis 30g, Radix Stemonae 20g, Rhizoma Coptidis 15g, decocting 2～3 times, merging filtrate is concentrated into 1000ml; Add 0.02%～0.05% Mentholum, 0.1～0.5% hyaluronic acid.(3) above-mentioned lyophilized powder is dissolved among the Chinese medicine extract 20ml uses.
Embodiment 7 repairs and renders a service experiment
Adopt chick embryo allantois (repair and render a service experiment) to carry out: to select 70 of fresh Embryo Gallus domesticus for use, be divided into 7 groups of experimental group, blank groups at random, every group each 5, give the spray 2ml of embodiment 1 to 6 respectively, matched group gives normal saline 2ml, observe down in magnifier after 7 days, matched group does not have new vessels, and experimental group has the new vessels growth.
The experiment of embodiment 8 antibacterial efficacies
Getting cultured staphylococcus aureus bacterium liquid 100 μ l evenly coats on the solid SCDLP flat board.With aseptic nipper sterilized little filter paper (diameter 5mm) is gently placed on the flat board, the spray with the above-mentioned example 1 to 6 of 5 μ l drips respectively on filter paper then, treat that sample permeates fully after, culture dish is inverted in 37 ℃ of incubators cultivated 6-8 hour.Take out culture dish, observe the inhibition zone size.The result: above-mentioned observation sample is the inhibition zone of visible diameter 8～10mm all.
Claims (6)
1. a vagina spray contains 1～100 μ g/ml basic fibroblast growth factor and protective agent thereof.
2. vagina spray as claimed in claim 1 is characterized in that containing 10～1,000,000 units/g interferon, and its interferon is α 2a type or α 1b type.
3. vagina spray as claimed in claim 2, it is characterized in that protective agent can select for use albumin, mannitol, chitin polysaccharide wherein one or both, also can be mixed and made into by multiple.
4. vagina spray as claimed in claim 3 is characterized in that containing 0.1～0.5% hyaluronic acid and 0.02%～0.05% Mentholum.
5. vagina spray as claimed in claim 4 is characterized in that containing Chinese medicine extract such as Fructus Cnidii, Cortex Phellodendri, Radix Sophorae Flavescentis, the Radix Stemonae, Rhizoma Coptidis.
6. preparation method for preparing the described vagina spray of claim 5, form by following steps:
(1) separately or mix the back with the water for injection dissolving, filter then, degerming, lyophilized powder is made in last lyophilization with basic fibroblast growth factor, interferon and/or albumin and/or mannitol and/or chitin polysaccharide; (2) face in time spent adding claim 4, the 5 described Chinese medicine extract solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001142968A CN1156307C (en) | 2000-05-23 | 2000-05-23 | Vagina spry containing biological active factor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001142968A CN1156307C (en) | 2000-05-23 | 2000-05-23 | Vagina spry containing biological active factor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1324659A true CN1324659A (en) | 2001-12-05 |
| CN1156307C CN1156307C (en) | 2004-07-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001142968A Expired - Fee Related CN1156307C (en) | 2000-05-23 | 2000-05-23 | Vagina spry containing biological active factor |
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| Country | Link |
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| CN (1) | CN1156307C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600456A (en) * | 2012-03-22 | 2012-07-25 | 兰州大学第二医院 | Bioadhesive vaginal plug for treating vaginitis and cervicitis as well as preparation method of bioadhesive vaginal plug |
| CN110917132A (en) * | 2019-12-19 | 2020-03-27 | 浙江因诺泰医疗器械有限公司 | Vaginal squamous epithelium repair aqua and preparation method thereof |
-
2000
- 2000-05-23 CN CNB001142968A patent/CN1156307C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600456A (en) * | 2012-03-22 | 2012-07-25 | 兰州大学第二医院 | Bioadhesive vaginal plug for treating vaginitis and cervicitis as well as preparation method of bioadhesive vaginal plug |
| CN102600456B (en) * | 2012-03-22 | 2014-05-07 | 兰州大学第二医院 | Bioadhesive vaginal plug for treating vaginitis and cervicitis as well as preparation method of bioadhesive vaginal plug |
| CN110917132A (en) * | 2019-12-19 | 2020-03-27 | 浙江因诺泰医疗器械有限公司 | Vaginal squamous epithelium repair aqua and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1156307C (en) | 2004-07-07 |
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