CN1318062C - Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof - Google Patents

Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof Download PDF

Info

Publication number
CN1318062C
CN1318062C CNB2004100732364A CN200410073236A CN1318062C CN 1318062 C CN1318062 C CN 1318062C CN B2004100732364 A CNB2004100732364 A CN B2004100732364A CN 200410073236 A CN200410073236 A CN 200410073236A CN 1318062 C CN1318062 C CN 1318062C
Authority
CN
China
Prior art keywords
extract
vitis viniferae
semen vitis
fructus sophorae
ethanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2004100732364A
Other languages
Chinese (zh)
Other versions
CN1634359A (en
Inventor
刘晓华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CNB2004100732364A priority Critical patent/CN1318062C/en
Publication of CN1634359A publication Critical patent/CN1634359A/en
Application granted granted Critical
Publication of CN1318062C publication Critical patent/CN1318062C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention relates to a composition and a preparing method thereof, which comprises locust fruit, grape pips and bark extract. The existing soybean isoflavone mainly comprises various flavonoid compound mixtures of genistin, soy bean glycoside, etc., and exists in a glycoside state. However, the soybean isoflavone can be absorbed and utilized by a human body through the decomposition of an intestinal canal of the human body, and the activity of the soy bean glycoside is much lower than that of the genistein. Moreover, osteoporosis therapeutic medicine comprises estrogen, but cancers caused by taking the estrogen in a long term are reported. The present invention relates to the composition of the extract of the locust fruit which comprises genistein and the extract of the grape pips and bark which comprise procyanidin. The present invention adopts a biochemical method that the locust fruit, the grape pips and the bark are used as raw materials for preparing medicine which has the healthcare effects of bone density increase, oxidation resistance, climacteric symptom relief, cardiovascular disease control, etc. The medicine is tested on 200 people, and clinical observation proves that overall therapeutic efficacy is good, and an effective rate reaches more than 95 percent. In addition, the present invention has the advantages of safety, high efficiency and no adverse reaction and side effects.

Description

A kind of composition and method of making the same that contains Fructus Sophorae, Semen Vitis viniferae, peel extract
One, technical field:
The present invention relates to a kind of composition and method of making the same that contains Fructus Sophorae, Semen Vitis viniferae, peel extract.Specifically adopting biochemical method, is raw material with Fructus Sophorae, Semen Vitis viniferae, skin, prepares a kind of medicine that has bone density improving, antioxidation, alleviation menopause syndrome, prevents and treats health-care effects such as cardiovascular disease.
Two, background technology:
Owing to the health oxidation resistance reduces the medicine that people, the osteoporotic people of needs control, the people that need prevent and treat cardiovascular disease who causes old and feeble and various degenerative disease, the women that menopause syndrome is arranged etc. need mitigate the disease.Existing product has the soybean isoflavone series products, its soybean isoflavone mainly contains the mixture of multiple flavone compounds such as genistin, daiazi, mainly the form with glycosides exists, need just can be absorbed by the people through the decomposition of human body intestinal canal enzyme, therefore individual absorption difference is bigger, and the activity of daiazi is well below genistein; And existing osteoporosis therapy medicine mainly contains following a few class: 1. estrogens; 2. Calcium Metabolism Regulation agent is as calcitonin etc.; 3. bisphosphonates; 4. the other types medicine comprises skeletal growth factor, fluoride and various calcium preparation.Taking estrogen for a long time causes cancer that existing report takes place; Calcium preparation just increases blood calcium, rather than increases bone formation, and the generally use of calcium preparation reduces fracture rates; Calcitonin costs an arm and a leg; Bisphosphonates has tangible side effect of digestive tract etc.; Fluoride will reach effective therapeutic effect, must take heavy dose (75mg) every day, and the treatment window is narrow, and the high dose administration also can weaken mineralization to the osteocyte toxigenicity, reduces the bone sedimentation rate and causes the osteomalacia to wait side effect.
Three, summary of the invention:
The object of the present invention is to provide and have bone density improving, antioxidation, alleviation menopause syndrome, prevent and treat the composition and method of making the same of health-care effects such as cardiovascular disease.
For achieving the above object, the technical solution used in the present invention is:
A kind of compositions that contains Fructus Sophorae, Semen Vitis viniferae, peel extract, its special character is: described compositions is made by following materials of weight proportions:
The Fructus Sophorae extract 1-3 part that contains genistein
Semen Vitis viniferae, peel extract 1-3 part of containing procyanidin
Above-mentioned a kind of compositions that contains Fructus Sophorae, Semen Vitis viniferae, peel extract, its special character is: described compositions is made by following materials of weight proportions:
1.5 parts of Fructus Sophorae extracts that contain genistein
1.5 parts of Semen Vitis viniferae, peel extract containing procyanidin
Above-mentioned a kind of preparation of compositions method that contains Fructus Sophorae, Semen Vitis viniferae, peel extract, its special character is:
The extracting method of the described Fructus Sophorae extract that contains genistein is as follows:
Fructus Sophorae drying, pulverizing were steamed 30 minutes with steamer, added the nutrient that is beneficial to the aspergillus oryzae growth, as 20% wheat bran, the inoculation aspergillus oryzae ferments under 30 ℃ clean environment, squeeze and filter then, filtering residue filter through 60 ℃ of extractions of 70% ethanol, concentrate and reclaim ethanol, extract falls through water precipitating, filters, ethyl alcohol recrystallization, spray drying promptly gets and contains genistein and reach Fructus Sophorae extract more than 90%, also contains have an appointment 6% rutin, 4% kaempferol etc. in extract.
The extracting method of the described Semen Vitis viniferae that contains procyanidin, peel extract is as follows:
Behind Semen Vitis viniferae, the skin cold compression to degrease, through 60 ℃ of extractions of 70% ethanol, filtration, the concentrated ethanol that reclaims, through 98% ethanol cold sedimentation, filter, extracting solution concentrates and reclaims ethanol, through polyamide column chromatography, and 70% ethanol elution, eluent concentrates the back spray drying and promptly gets and contain procyanidin and reach Semen Vitis viniferae, peel extract more than 70%, also contains 30% the polyhydric phenols of having an appointment etc. in extract.
Fructus Sophorae extract and Semen Vitis viniferae, peel extract are pressed 1-3: the mixed of 1-3 adds existing adjuvant and makes pharmaceutical formulation.
Above-mentioned pharmaceutical formulation is sheet glue, capsule, electuary.
The present invention is with respect to prior art, and its advantage is as follows:
1, take medicine of the present invention after, the trend that the intravital SOD contents level of people significantly raises, glutathione content has rising, the contents level of lipid peroxide descend, skin improves, resistance increases, osteoporosis such as cramp in the leg, aching pain in waist and back symptom alleviates or disappears, blood fat and blood pressure is steady, and menopause syndrome such as vexed insomnia alleviate or disappear.
2, the present invention is used for 200 people, and through clinical observation, overall therapeutic effect is good, and effective percentage reaches more than 95%.
3, the present invention detects through the disease prevention and control center, Shaanxi Province, and untoward reaction and side effect do not appear in safety, effective.
4, the present invention avoids too much with an organic solvent, helps protecting environment.
Four, specific embodiment:
The present invention is applicable to medicine of the present invention to reduce people, the osteoporotic people of needs control, the people that need prevent and treat cardiovascular disease who causes old and feeble and various degenerative disease, the women that menopause syndrome is arranged etc. owing to the health oxidation resistance.
It is as follows that the present invention contains the extracting method of Fructus Sophorae extract of genistein: Fructus Sophorae drying, pulverizing, steamed 30 minutes with steamer, and add the nutrient that is beneficial to the aspergillus oryzae growth, as 20% wheat bran, the inoculation aspergillus oryzae ferments under 30 ℃ clean environment, squeeze and filter then, filtering residue filter through 60 ℃ of extractions of 70% ethanol, concentrate and reclaim ethanol, extract falls through water precipitating, filters ethyl alcohol recrystallization, spray drying promptly gets and contains genistein and reach Fructus Sophorae extract more than 90%.
It is as follows that the present invention contains the extracting method of Semen Vitis viniferae, peel extract of procyanidin:
Behind Semen Vitis viniferae, the skin cold compression to degrease, through 60 ℃ of extractions of 70% ethanol, filtration, the concentrated ethanol that reclaims, through 98% ethanol cold sedimentation, filter, extracting solution concentrates and reclaims ethanol, through polyamide column chromatography, 70% ethanol elution, eluent concentrate the back spray drying and promptly get and contain procyanidin and reach Semen Vitis viniferae, peel extract more than 70%.
Fructus Sophorae extract and Semen Vitis viniferae, peel extract are pressed 1-3: the mixed of 1-3 adds existing adjuvant and makes pharmaceutical formulation.
The present invention is in the extraction process of Fructus Sophorae extract; the present invention quotes the biochemistry means; utilize microorganism that the chemical constituent of raw material is degraded; need not pass through hydrolysis, destroy glycosidic bond, make glycosides be transformed into aglycon; the extraction rate of effective ingredient is increased more than the twice; and avoid excessive heat and acid hydrolysis destruction, also avoid too much with an organic solvent, help protecting environment effective ingredient.
The content of genistein is up to more than 90% in the Fructus Sophorae extract in the prescription, effective ingredient is clear and definite, the mechanism of action is clear, consumption is controlled, the estrogen activity of genistein can effectively be alleviated the Woman climacteric symptom, also has bone density improving, antioxidation, prevents and treats the health-care effect of cardiovascular disease etc.Contain the procyanidin more than 70% in Semen Vitis viniferae, the peel extract, procyanidin is the most powerful antioxidant of finding up to now, and its oxidation resistance is ascorbic 30 times, 50 times of vitamin E, but blood fat reducing also.Both economic benefits and social benefits unification, reinforced effects more mutually.
The present invention has bone density improving, antioxidation, alleviation menopause syndrome, prevents and treats health-care effects such as cardiovascular disease.
For verifying its anti-oxidation function clinical effectiveness, by Shaanxi Province Centre for Disease Control the present invention is done the anti-oxidation function test with rat, show that the rat anti oxidase active has two index determining results all positive, two results of lipid peroxide assay are also all positive, so judge that the present invention has anti-oxidation function.
By Zhengzhou Railway Administration Xi'an central hospital, Shaanxi Province Centre for Disease Control the present invention has been done the report of health food human experiment, its testing result is as follows: the test-meal group is after taking three months continuously, SOD content raises in experimenter's serum, self relatively reach with the matched group comparing difference before and after the test significance (p<0.01, p<0.05) is all arranged.Experimenter's hemoglobin, erythrocyte, leukocyte, glutamate pyruvate transaminase, glutamic oxaloacetic transaminase, GOT, blood urea nitrogen, creatinine, total protein, the every index test of albumin front and back are all in normal range.Urine before and after experimenter's test-meal, just routine examination no abnormality seen, Chest X-rays, electrocardiogram, Abdominal B type ultrasonography inspection also there is no unusually, and experimenter front and back meals do not have obvious change.Can judge that by above-mentioned result the present invention has anti-oxidation function.
For verifying its bone density improving function clinical effectiveness, 200403033 the examining report of having done by nutrition of Chinese Centre for Disease Control and food safety, its testing result is as follows: result of the test shows, the present invention is to basic, normal, high dosage group rat femur weight in wet base. and relatively there were significant differences (P<0.05 or P<0.01) for dry weight, bone density, bone mineral and calcium content of bone and model control group, regulation in the foundation " health food detects and assessment technique standard-2003 " thinks that the present invention has the effect of increase rat bone density function.
By Shaanxi Province Centre for Disease Control the toxicology test that the present invention does is shown:
1, rat acute per os toxicity test, all greater than 15.0g/kg.bw, its dosage is equivalent to 1500 times of human body recommended amounts to female, great and mighty or powerful Mus per os maximum tolerated dose in the present invention.
2, genetic toxicity test: mouse marrow cell micro nuclear test, mouse sperm deformity test, three results are all negative for Salmonella reversion test, i.e. and the present invention does not see the genetoxic effect.
3,30 days feeding trials of rat: in test dose 0.25g/kg.bw-1.00g/kg.bw scope, the growth promoter, hematology, blood biochemical of test rat are learned and obvious adverse reaction is not all found in the check of the every index in pathology aspect.
Concrete case:
1 name: Zhang Zhuanhe age: 53 years old
Take preceding malaise symptoms: xerosis cutis, menopause syndrome such as vexed, Tidal fever with perspiration of having a sleepless night is obvious, aching pain in waist and back.
Take the back: the skin smooth exquisiteness that becomes, sleep quality improves, and can one feels and sleeps to daybreak, and menopause syndrome such as vexed, temperish, Tidal fever with perspiration disappear, and in high spirits, the aching pain in waist and back situation is alleviated.
2 names: Li Bo age: 36 years old
Take preceding malaise symptoms: xerosis cutis has mottle, constipation.
Take the back: the skin smooth exquisiteness that becomes, mottle is thin out, and constipation disappears.
3 names: Huang Xiaorong age: 48 years old
Take preceding malaise symptoms: the blood fat height, hypertension has heart disease, and heart beating is slow excessively, and often suppress awake night.
Take the back: blood fat becomes normally, and blood pressure is normal, and nighttime sleep is good, and the phenomenon of feeling suffocated disappears.
Embodiment 1:
1.5 kilograms of Fructus Sophorae extracts that contain genistein
1.5 kilograms of Semen Vitis viniferae, peel extract containing procyanidin
The extracting method of the described Fructus Sophorae extract that contains genistein is as follows:
Fructus Sophorae drying, pulverizing were steamed 30 minutes with steamer, added the nutrient that is beneficial to the aspergillus oryzae growth, as 20% wheat bran, the inoculation aspergillus oryzae ferments under 30 ℃ clean environment, squeeze and filter then, filtering residue filter through 60 ℃ of extractions of 70% ethanol, concentrate and reclaim ethanol, extract falls through water precipitating, filters, ethyl alcohol recrystallization, spray drying promptly gets and contains genistein and reach 90% Fructus Sophorae extract, also contains 6% rutin, 4% kaempferol in extract.
The extracting method of the described Semen Vitis viniferae that contains procyanidin, peel extract is as follows:
Behind Semen Vitis viniferae, the skin cold compression to degrease, through 60 ℃ of extractions of 70% ethanol, filtration, the concentrated ethanol that reclaims, through 98% ethanol cold sedimentation, filter, extracting solution concentrates and reclaims ethanol, through polyamide column chromatography, and 70% ethanol elution, eluent concentrates the back spray drying and promptly gets and contain procyanidin and reach 70% Semen Vitis viniferae, peel extract, also contains 30% polyhydric phenols in extract.
Fructus Sophorae extract and Semen Vitis viniferae, peel extract are pressed 1-3: the mixed of 1-3 adds existing adjuvant and makes pharmaceutical formulation.
Embodiment 2:
1 kilogram of the Fructus Sophorae extract that contains genistein
1 kilogram of Semen Vitis viniferae, peel extract containing procyanidin
Its extracting method is identical with the method for embodiment 1.
Embodiment 3:
3 kilograms of Fructus Sophorae extracts that contain genistein
3 kilograms of Semen Vitis viniferae, peel extract containing procyanidin
Its extracting method is identical with the method for embodiment 1.

Claims (3)

1, a kind of compositions that contains Fructus Sophorae, Semen Vitis viniferae, peel extract, it is characterized in that: the active component of described compositions is made by following materials of weight proportions:
Contain genistein and reach Fructus Sophorae extract 1-3 part of 90%
Contain procyanidin and reach Semen Vitis viniferae, peel extract 1-3 part of 70%
Describedly contain genistein to reach the extracting method of 90% Fructus Sophorae extract as follows:
Fructus Sophorae drying, pulverizing were steamed 30 minutes with steamer, added 20% wheat bran, the inoculation aspergillus oryzae ferments under 30 ℃ clean environment, then squeeze and filter, filtering residue is through 60 ℃ of extractions of 70% ethanol, filter, concentrate and reclaim ethanol, extract falls through water precipitating, filter, ethyl alcohol recrystallization, spray drying promptly gets and contains genistein and reach 90% Fructus Sophorae extract.
Describedly contain procyanidin to reach the extracting method of 70% Semen Vitis viniferae, peel extract as follows:
Behind Semen Vitis viniferae, the skin cold compression to degrease, through 60 ℃ of extractions of 70% ethanol, filtration, the concentrated ethanol that reclaims, through 98% ethanol cold sedimentation, filter, extracting solution concentrates and reclaims ethanol, through polyamide column chromatography, 70% ethanol elution, eluent concentrate the back spray drying and promptly get and contain procyanidin and reach 70% Semen Vitis viniferae, peel extract.
Described Fructus Sophorae extract and Semen Vitis viniferae, peel extract are mixed in proportion, add existing adjuvant and make pharmaceutical formulation.
2, a kind of compositions that contains Fructus Sophorae, Semen Vitis viniferae, peel extract according to claim 1, it is characterized in that: described compositions active component is made by following materials of weight proportions:
With described Fructus Sophorae extract and Semen Vitis viniferae, peel extract mixed, add existing adjuvant and make pharmaceutical formulation by 1.5: 1.5.
3, a kind of compositions that contains Fructus Sophorae, Semen Vitis viniferae, peel extract according to claim 1 and 2, it is characterized in that: described pharmaceutical formulation is sheet glue, capsule, electuary.
CNB2004100732364A 2004-11-04 2004-11-04 Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof Expired - Fee Related CN1318062C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2004100732364A CN1318062C (en) 2004-11-04 2004-11-04 Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100732364A CN1318062C (en) 2004-11-04 2004-11-04 Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof

Publications (2)

Publication Number Publication Date
CN1634359A CN1634359A (en) 2005-07-06
CN1318062C true CN1318062C (en) 2007-05-30

Family

ID=34846812

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB2004100732364A Expired - Fee Related CN1318062C (en) 2004-11-04 2004-11-04 Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof

Country Status (1)

Country Link
CN (1) CN1318062C (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102628227A (en) * 2012-03-29 2012-08-08 江南大学 Grape-seed-based ecological preparation method for ultravioresistant woolen and silk textiles
CN105503980B (en) * 2015-12-01 2018-05-11 大兴安岭林格贝寒带生物科技股份有限公司 A kind of extracting method of grape peel anthocyanidin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87107111A (en) * 1987-10-23 1988-06-15 衡水市还童茶饮料厂 The preparation method of medical health drinking agent

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87107111A (en) * 1987-10-23 1988-06-15 衡水市还童茶饮料厂 The preparation method of medical health drinking agent

Also Published As

Publication number Publication date
CN1634359A (en) 2005-07-06

Similar Documents

Publication Publication Date Title
Bhowmik et al. A potential medicinal importance of zinc in human health and chronic
JP4609875B2 (en) healthy food
CN104799146A (en) Functional nutritional good composition for improving sub-health and preparation method thereof
CN102406860A (en) Composition for preventing and treating diabetes, and preparation method and use thereof
JP2020172523A (en) Eurycoma longifolia extract and its use in enhancing and/or stimulating immune system
CN101288686A (en) Health preparation for increasing bone density and preparation method thereof
CN105942498A (en) Functional nutritional food compounds with diabetes-treating effects and preparation methods thereof
CN100366628C (en) Effective parts of fructus sophorae flavone production and use thereof
CN107455625A (en) A kind of nutrient for plants beverage and its preparation technology with antifatigue weight losing function
JP2007523157A (en) Cold prevention composition
JP4553224B2 (en) healthy food
CN111771988A (en) Nutritional food suitable for diabetic patients and preparation method thereof
CN110772578A (en) Human body immunity enhancer for preventing and treating cancer and preparation method thereof
CN109077321A (en) A kind of composition and preparation method thereof with strengthen immunity and antioxidation
CN1799582A (en) Blood sugar- and blood pressure-lowering medicine
CN1318062C (en) Composition containing extracts of pagodatree fruits, grape seeds and peel and preparation method thereof
KR102003755B1 (en) Germinated Fermented Soybean Extract Having Effect of Protecting Liver Cells and Increasing Bone Density Containing Coumesterol, And Composition Containing The Extract
CN107772468A (en) The functional food that a kind of women takes
CN104001157A (en) Compound preparation for preventing and treating osteoporosis and preparation method thereof
RU2408383C1 (en) Composition with antineoplastic and adaptogenic activity (versions) and based drug (versions)
CN107668695A (en) A kind of Solid nutritional electuary for preventing cardiovascular and cerebrovascular disease and preparation method thereof
CN103005439A (en) Bitter gourd compound lipid-lowering oral solution and preparation method thereof
CN101933968A (en) Pharmaceutical composition with functions of resisting oxidation and delaying senility and preparation method thereof
CN102125129A (en) Health care tea
CN112076305A (en) A Chinese medicinal oral and topical stock solution mixture for treating metabolic syndrome and resisting bacteria and inflammation

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20070530

Termination date: 20121104