CN1317392C - Corn protein high F value oligopeptide production method - Google Patents
Corn protein high F value oligopeptide production method Download PDFInfo
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Abstract
The present invention relates to a production method of high F-value oligopeptide of zein, which belongs to the processing technology of health care foods. Corn yellow powders are used as raw materials, supercritical CO2 fluid extraction is carried out, and zein is extracted after lipid substances and substances with an abnormal and foreign taste are eliminated from the corn yellow powders. Water wet materials in an appropriate amount are added to the zein, and extrusion modification treatment is carried out at certain temperature and pressure; thereby, the degree of solubility is increased. Two kinds of proteinase are used for the implementation of two-step enzymolysis on the zein of modification treatment under the conditions of certain concentration of substrate, enzyme concentration, pH value and time, and then separation, purification, drying and pulverization are carried out; thereby, high F-value zein oligopeptide of which the F-value is higher than 20 is obtained. Compared with traditional methods, the method of the present invention can increase the extraction rate of zein by at least 10%, and the degree of hydrolysis of zein can be increased by at least 9%. Raw materials of the present invention are fully utilized in the process of manufacturing high F-value oligopeptide of zein, and the present invention has the advantages of no generation of waste slag and waste steam and no environmental pollution.
Description
Technical field
The invention belongs to health food processing technology.
Background technology
In aminoacid mixture, branched-chain amino acid (Branched chain amino acid is called for short BCAA) is called Fiscer with the mol ratio of die aromatischen Aminosaeuren (Aromatic amino acid is called for short AAA), is called for short the F value.High F value oligopeptide is that proteolytic enzyme acts on a kind of lower molecular weight physiologically active peptide with special physiological function that forms behind the protein, is hepatic diseases patient's nutritious supplementary and food ingredients.Modern medicine and trophology studies have shown that high F value oligopeptide has the proteinaceous nutrient situation that suppresses hepatogenic encephalopathy, improves the liver patient, the function that improves body resisting fatigue ability.Have employings such as document announcement nineteen ninety Shin-ya T. to have a liking for the high F value oligopeptide that basonuclin enzyme and Actin muscle enzyme liberating zein are developed no bitter taste, yield reaches 56%, F value 20.47, and AAA content is 1.86%.Shuji A. in 1992 etc. are that raw material is produced high F value oligopeptide mixture with the bovine casein.High F value oligopeptide has good local flavor, and abundant nutrition is is easily digested and assimilated, and the treatment hepatogenic encephalopathy is had remarkable efficacy.But the research in this field at present is fully matured not as yet, relevant its through engineering approaches technology, and industrialization method also rests on experimental size.As a kind of functional food ingredient, should develop raw material resources to greatest extent, enlarge its throughput, really can be widely used in the production of correlation function food to it, for proteinaceous nutrient and the health level of improving the whole people are brought into play due effect.
Corn is one of the world three generalized grain crops, not only in agriculture production, occupies an important position, and also the main raw material of starch, glucose, Alcohol Production in industrial production.Contain materials such as starch, carbohydrate, protein, mineral substance, fiber in the corn, protein content average out to about 12% is higher than other cereal crop.Corn protein has four kinds: prolamine, gluten, albumin, sphaeroprotein.The main byproduct that the wet production W-Gum produces is maize yellow-powder (Zein powder), and its main component is a zein.Prolamine has unique solvability, and it is water insoluble, also is insoluble to anhydrous alcohols, but can be dissolved in the aqueous ethanolic solution of 60%-95%, contain a large amount of hydrophobic amino acids in the prolamine molecule, have very big commercial value, become the focus of countries nowadays scholar's research.But undressed maize yellow-powder color is pale brown, has the different assorted smell of intensive, and the lipid content height has stronger detrimentally affect to the extraction and the enzymolysis of prolamine.For the extraction yield of raising zein and the quality of proteolysate, should remove its contained lipid and different assorted flavor material to greatest extent.Ordinary method generally adopts enzyme process, alkali cleaning, organic solvent processing etc.The enzyme process complex operation is easily introduced heterologous protein to product, and quality product is wayward; Alkaline process and organic solvent are handled and are easily brought chemical pollution to product.Supercritical CO
2Fluid has the two characteristic of liquids and gases concurrently, has good penetrance and stripping property, supercritical CO
2Fluid extraction has easy to operate, and selectivity is good, and the degreasing rate height has the different assorted flavor effect of taking off concurrently, and advantages such as extraction agent is nontoxic, noresidue can obviously improve productive rate, purity and the quality of product, and energy-conservation, save time, but automated operation.
Zein is water insoluble, handles as not adopting appropriate means that it is carried out solubilising, produces high F value oligopeptide and it directly is used for enzymolysis, and then enzymolysis efficiency is extremely low.The tradition solubilizing methods such as reductive agent, acid, alkali, thermal treatment that adopt more, complex operation, and easily bring chemical pollution.
Summary of the invention
It is the method that raw material is produced corn protein high F value oligopeptide with the maize yellow-powder that the present invention aims to provide a kind of.The technical scheme that the present invention takes is: comprise the following steps:
At first maize yellow-powder is adopted supercritical CO
2Fluid extraction technology removes lipid and different assorted flavor material, extraction operating pressure 10MPa～45MPa, 35 ℃～60 ℃ of temperature, 0.5 hour～3.0 hours time, CO
2Flow 15～40L/ hour, the maize yellow-powder color after the extraction was yellowish, was as good as assorted flavor, and lipid material content is less than 0.2%; With degreasing, to take off maize yellow-powder concentration after assorted be that 60%～90% ethanol extracts, the mass ratio of maize yellow-powder and ethanolic soln is 1: 8～12, the extraction temperature is 50 ℃～60 ℃, pH value 8.0～9.0; With extract separate, dehydration, dry and pulverize the zein powder.This protein powder protein content is greater than 95%;
The zein powder being added the suitable quantity of water material moistening handles again, make its water content 15%～30%, carry out High Temperature High Pressure in the zein powder input forcing machine with the material moistening processing and extrude processing, 120 ℃～160 ℃ of extrusion temperatures, input speed 15～40kg/ hour, the material after extruding is carried out drying, make the extrudate water content less than 8%, dried material is pulverized, and gained meal granularity is controlled at can all get final product by 100 mesh sieves;
Utilize proteolytic enzyme that zein is carried out two step enzymolysis at last.The 1st step enzymolysis is selected for use and the protein and peptide chain can be cut into small peptide and the cutting position overwhelming majority proteolytic enzyme in the junction of die aromatischen Aminosaeuren and branched-chain amino acid, making its end of small peptide that generates behind the enzymolysis is die aromatischen Aminosaeuren, processing condition are concentration of substrate 5%～13%, enzyme concn 1.5～9.0%, 45 ℃～60 ℃ of temperature, pH value 8.0～10.0,2 hours～5 hours time, separate behind the enzymolysis enzymolysis solution; The 2nd step enzymolysis is selected the proteolytic enzyme that the die aromatischen Aminosaeuren of small peptide end can be cut away for use, and processing condition are 45 ℃～60 ℃ of temperature, enzyme concn 1%～9%, concentration of substrate 5%～13%, 2～6 hours time, pH value 6.5～9.0; For removing the bitter taste of corn protein high F value oligopeptide, also can be behind the 2nd step enzymolysis, the flavor protease of interpolation 0.5% carries out enzymolysis once more in enzymolysis solution, and enzymatic hydrolysis condition is 55 ℃ of temperature, pH value 7.0,1 hour time; After enzymolysis finishes, obtain containing the mixture of die aromatischen Aminosaeuren, branched-chain amino acid and oligopeptide, utilize this mixture of charcoal absorption purifying, remove die aromatischen Aminosaeuren.Charcoal absorption purifying process condition is 20 ℃～35 ℃ of temperature, and the mass ratio of gac and liquid to be purified is 1: 5～15, pH value 2.5～5.0.
Zein oligopeptide mixture by the inventive method is produced does not have bitter taste, and the F value is greater than 20, and the product yield is greater than 60%.
The supercritical CO that the present invention adopts
2Fluid extraction technology has the high advantage of degreasing rate, have the different assorted flavor effect of taking off simultaneously concurrently, improved the extraction yield of zein, compare with alkaline process, acid system and enzyme process have efficient, low consumption, nontoxic, pollution-free, unharmful substance is residual, easy to operate, good selective, and can make production serialization, automatization, compares with traditional method, can enhance productivity more than 1 times, take off in the degreasing of maize yellow-powder and have the additive method incomparable advantage aspect the assorted purifying.The present invention adopts the High Temperature High Pressure expressing technique to improve the enzymolysis property of zein, realizes the modified and solubilized processing to material.High temperature, when high pressure is extruded, zein is produced powerful friction, shear, the fusion Degradation, make the moisture instant vaporization of superheat state contained in the material and produce blast effectiveness, break or cut off the insoluble long chain molecule of zein, reduce its molecular weight limitedly, and molecule modified, change zein part chemical structure and relative molecular weight, destroyed the hard tissues of zein, made it become loose, solubility strengthens, enzyme such as peptide bond and hydrophilic radical point of application comes out, increase with the enzyme contact area, more it is easily decomposed by enzyme, and then increases substantially the degree of hydrolysis of zein, and this easy grasp easy and simple to handle, energy-conservation, no waste gas, waste water produces, and belongs to the environmental protection operation.
With the maize yellow-powder is raw material, adopt the inventive method to produce high F value oligopeptide, the zein extraction yield improves 10% at least, produce high F value oligopeptide with high purity and through the zein of extruding modified and solubilized processing through complex enzyme hydrolysis, compare the zein degree of hydrolysis with traditional method and improve 23% at least, the high F value oligopeptide yield can improve more than 20%.
Embodiment
By product maize yellow-powder with W-Gum factory or corn distillery is a raw material.
Embodiment 1
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 10MPa, 35 ℃ of temperature, 0.5 hour time, CO
2Flow 15L/ hour.
2. the extraction of zein
With supercritical CO
2Maize yellow-powder behind the fluid extraction is a raw material, at alcohol concn is 60%, maize yellow-powder and ethanolic soln mass ratio are 1: 8, extracting temperature is 50 ℃, extract under the condition of pH value 8.0 and got zein solution in 2 hours, centrifugation 5min under the condition of rotating speed 3000rpm gets supernatant liquor with zein solution, be 30% with supernatant liquor with distilled water diluting to alcohol concn and be 6.5 when having turbid phenomenon to produce with 4mol HCI solution adjustment pH value, the saturated nacl aqueous solution that adds cumulative volume 2% promotes the prolamine precipitation to separate out, centrifugation 5min under rotating speed 4000rpm condition, separate and obtain the zein throw out, with throw out with pure washing 2 times, vacuum-drying then, pulverize the zein powder.
3. material moistening is handled
The zein powder is added an amount of 25 ℃ tap water, stir, placed 15 minutes, the zein powder is fully absorbed water, and guarantee that its water content is 15%.
4. High Temperature High Pressure is extruded processing
With the single screw extrusion machine preheating, 120 ℃ of design temperatures, when die head temperature reached 120 ℃, the starting forcing machine was extruded operation, control input speed 15kg/ hour, in the extrusion, displays temperature should not be lower than 120 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 5%, enzyme concn (E/S) 1.5%, 45 ℃ of temperature, pH value 8.0,2 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 45 ℃ of temperature, enzyme concn (E/S) 1%, concentration of substrate 5%, pH value 6.5,2 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 20 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 5, pH value 2.0 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 2
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 20MPa, 45 ℃ of temperature, 1 hour time, CO
2Flow 20L/ hour.
2. the extraction of zein
With supercritical CO
2Maize yellow-powder behind the fluid extraction is a raw material, be 70% at alcohol concn, maize yellow-powder and ethanolic soln mass ratio be 1: 9, extract temperature be extract under 55 ℃, the condition of pH value 8.5 2 hours must zein solution, as follows embodiment 1.
3. material moistening is handled
With adding an amount of 25 ℃ tap water in the zein powder, stir, placed 15 minutes, the zein powder is fully absorbed water, and guarantee that its water content is 20%.
4. High Temperature High Pressure is extruded processing
With the twin screw extruder preheating, 130 ℃ of design temperatures, when die head temperature reached 130 ℃, the starting forcing machine was extruded operation, control input speed 20kg/ hour, in the extrusion, displays temperature should not be lower than 130 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 7%, enzyme concn (E/S) 3.0%, 50 ℃ of temperature, pH value 9.0,3 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 50 ℃ of temperature, enzyme concn (E/S) 3%, concentration of substrate 7%, pH value 7.5,3 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 25 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 7, pH value 3.0 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 3
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 30MPa, 45 ℃ of temperature, 1.5 hours time, CO
2Flow 25L/ hour.
2. the extraction of zein
With supercritical CO
2Maize yellow-powder behind the fluid extraction is a raw material, be 80% at alcohol concn, maize yellow-powder and ethanolic soln mass ratio be 1: 10, extract temperature be extract under 60 ℃, the condition of pH value 9.0 2 hours must zein solution, as follows embodiment 1.
3. material moistening is handled
With adding an amount of 25 ℃ tap water in the zein powder, stir, placed 15 minutes, the zein powder is fully absorbed water, and guarantee that its water content is 25%.
4. High Temperature High Pressure is extruded processing
With the twin screw extruder preheating, 140 ℃ of design temperatures, when die head temperature reached 140 ℃, the starting forcing machine was extruded operation, control input speed 25kg/ hour, in the extrusion, displays temperature should not be lower than 140 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 9%, enzyme concn (E/S) 4.5%, 55 ℃ of temperature, pH value 9.0,4 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 55 ℃ of temperature, enzyme concn (E/S) 5%, concentration of substrate 9%, pH value 7.5,4 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 30 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 9, pH value 3.0 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 4
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 40MPa, 50 ℃ of temperature, 2.0 hours time, CO
2Flow 30L/ hour.
2. the extraction of zein
With supercritical CO
2Maize yellow-powder behind the fluid extraction is a raw material, be 90% at alcohol concn, maize yellow-powder and ethanolic soln mass ratio be 1: 11, extract temperature be extract under 60 ℃, the condition of pH value 9.0 2 hours must zein solution, as follows embodiment 1.
3. material moistening is handled
The zein powder is added an amount of 25 ℃ tap water, stir, placed 15 minutes, the zein powder is fully absorbed water, and guarantee that its water content is 30%.
4. High Temperature High Pressure is extruded processing
With the single screw extrusion machine preheating, 150 ℃ of design temperatures, when die head temperature reached 150 ℃, the starting forcing machine was extruded operation, control input speed 30kg/ hour, in the extrusion, displays temperature should not be lower than 150 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 11%, enzyme concn (E/S) 6.0%, 60 ℃ of temperature, pH value 10,5 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 60 ℃ of temperature, enzyme concn (E/S) 7%, concentration of substrate 11%, pH value 8.0,5 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 35 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 11, pH value 3.5 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 5
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 45MPa, 55 ℃ of temperature, 2.5 hours time, CO
2Flow 35L/ hour.
2. the extraction of zein
With supercritical CO
2Maize yellow-powder behind the fluid extraction is a raw material, be 90% at alcohol concn, maize yellow-powder and ethanolic soln mass ratio be 1: 12, extract temperature be extract under 60 ℃, the condition of pH value 9.0 2 hours must zein solution, as follows embodiment 1.
3. material moistening is handled
With embodiment 4.
4. High Temperature High Pressure is extruded processing
With the single screw extrusion machine preheating, 160 ℃ of design temperatures, when die head temperature reached 160 ℃, the starting forcing machine was extruded operation, control input speed 35kg/ hour, in the extrusion, displays temperature should not be lower than 160 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 13%, enzyme concn (E/S) 7.5%, 60 ℃ of temperature, pH value 10,5 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 60 ℃ of temperature, enzyme concn (E/S) 9%, concentration of substrate 13%, pH value 8.5,6 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 35 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 13, pH value 4.5 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 6
1. maize yellow-powder degreasing, take off different assorted flavor and handle
Maize yellow-powder is carried out supercritical CO
2Fluid extraction, extracting pressure 45MPa, 60 ℃ of temperature, 3.0 hours time, CO
2Flow 40L/ hour.
2. the extraction of zein
With embodiment 5.
3. material moistening is handled
With embodiment 4.
4. High Temperature High Pressure is extruded processing
With the single screw extrusion machine preheating, 160 ℃ of design temperatures, when die head temperature reached 160 ℃, the starting forcing machine was extruded operation, control input speed 40kg/ hour, in the extrusion, displays temperature should not be lower than 160 ℃, with the extrudate that obtains, carry out warm air drying or natural air drying, make its water content less than 8%, pulverize then, make the meal that obtains can be all by 100 mesh sieves.
5. enzymolysis and separation and purification
1. Sumizyme MP enzymolysis: with the zein of extruding processing is that raw material carries out enzymolysis, and concentration of substrate is 13%, enzyme concn (E/S) 9.0%, 60 ℃ of temperature, pH value 10,5 hours time.Separate then enzymolysis solution.
2. papain enzymolysis: with the Sumizyme MP enzymolysis product is raw material, 60 ℃ of temperature, enzyme concn (E/S) 9%, concentration of substrate 9%, pH value 9.0,6 hours time.
3. Activated Carbon Adsorption Separation purifying: with step 2. products therefrom carry out the charcoal absorption purifying, 35 ℃ of temperature, the mass ratio of gac and pending enzymolysis solution is 1: 15, pH value 5.0 is removed die aromatischen Aminosaeuren to greatest extent, keeps the branched-amino acid constituents.
Embodiment 7
1. maize yellow-powder degreasing, take off different assorted flavor and handle
With embodiment 6.
2. the extraction of zein
With embodiment 5.
3. material moistening is handled
With embodiment 4.
4. High Temperature High Pressure is extruded processing
With embodiment 6.
5. enzymolysis and separation and purification
Behind the papain enzymolysis, the flavor protease of interpolation 0.5% carries out enzymolysis in enzymolysis solution, and enzymatic hydrolysis condition is 55 ℃ of temperature, pH value 7.0,1 hour time.
All the other contents are with embodiment 6.
Claims (3)
1, a kind of production method of corn protein high F value oligopeptide comprises the following steps:
At first maize yellow-powder is adopted supercritical CO
2Fluid extraction technology removes lipid and different assorted flavor material, extraction operating pressure 10MPa～45MPa, 35 ℃～60 ℃ of temperature, 0.5 hour～3.0 hours time, CO
2Flow 15～40L/ hour, the maize yellow-powder color after the extraction was yellowish, was as good as assorted flavor, and lipid material content is less than 0.2%; With degreasing, to take off maize yellow-powder concentration after assorted be that 60%～90% ethanol extracts, the mass ratio of maize yellow-powder and ethanolic soln is 1: 8～12, the extraction temperature is 50 ℃～60 ℃, pH value 8.0～9.0; With extract separate, dehydration, dry and pulverize the zein powder, this protein powder protein content is greater than 95%;
The zein powder being added the suitable quantity of water material moistening handles again, make its water content 15%～30%, carry out High Temperature High Pressure in the zein powder input forcing machine with the material moistening processing and extrude processing, 120 ℃～160 ℃ of extrusion temperatures, input speed 15～40kg/ hour, the material after extruding is carried out drying, make the extrudate water content less than 8%, dried material is pulverized, and gained meal granularity is controlled at can all get final product by 100 mesh sieves;
Utilize proteolytic enzyme that zein is carried out two step enzymolysis at last: the 1st step enzymolysis is selected for use and the protein and peptide chain can be cut into small peptide and the cutting position overwhelming majority proteolytic enzyme in the junction of die aromatischen Aminosaeuren and branched-chain amino acid, making its end of small peptide that generates behind the enzymolysis is die aromatischen Aminosaeuren, processing condition are concentration of substrate 5%～13%, enzyme concn 1.5～9.0%, 45 ℃～60 ℃ of temperature, pH value 8.0～10.0,2 hours～5 hours time, separate behind the enzymolysis enzymolysis solution; The 2nd step enzymolysis is selected the proteolytic enzyme that the die aromatischen Aminosaeuren of small peptide end can be cut away for use, and processing condition are 45 ℃～60 ℃ of temperature, enzyme concn 1%～9%, concentration of substrate 5%～13%, 2～6 hours time, pH value 6.5～9.0; After enzymolysis finishes, obtain containing the mixture of die aromatischen Aminosaeuren, branched-chain amino acid and oligopeptide, utilize this mixture of charcoal absorption purifying, remove die aromatischen Aminosaeuren, charcoal absorption purifying process condition is 20 ℃～35 ℃ of temperature, the mass ratio of gac and liquid to be purified is 1: 5～15, pH value 2.5～5.0.
2, the production method of corn protein high F value oligopeptide according to claim 1 is characterized in that: utilize proteolytic enzyme that zein is carried out two step enzymolysis: the 1st step enzymolysis is selected Sumizyme MP for use, and the 2nd step enzymolysis is selected papoid for use.
3, the production method of corn protein high F value oligopeptide according to claim 1 and 2, it is characterized in that: behind the 2nd step enzymolysis, the flavor protease of interpolation 0.5% carries out enzymolysis once more in enzymolysis solution, and enzymatic hydrolysis condition is 55 ℃ of temperature, pH value 7.0,1 hour time.
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| CN103766577B (en) * | 2014-02-28 | 2015-05-13 | 北京金地三福膨化机制造(大厂)有限公司 | Method for preparing active proteins from soybean hulls and application of active proteins |
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| 高F值寡肽混合物的制备及抗疲劳与抗缺氧作用 谷文英等，粮油食品科技，第7卷 1999 * |
| 高F值寡肽混合物的制备及抗疲劳与抗缺氧作用 谷文英等，粮油食品科技，第7卷 1999;酶解玉米蛋白粉制备高F值寡肽 谷文英等，精红与专用化学品，第21卷 2001;速溶黄粉蛋白乳粉的研制 任国谱，谷文英，中国油脂，第26卷第5期 2001 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108634236A (en) * | 2018-05-16 | 2018-10-12 | 浙江海润达科技有限公司 | A kind of preparation method of ultralow fat activity squid-soybean mixing peptide oral liquid |
| CN108634236B (en) * | 2018-05-16 | 2021-07-30 | 浙江海润达科技有限公司 | Preparation method of ultra-low fat active squid-soybean mixed peptide oral liquid |
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