CN1305388C - Tea beverage containing nanometer SOD and its preparation method - Google Patents

Tea beverage containing nanometer SOD and its preparation method Download PDF

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CN1305388C
CN1305388C CNB2004100209865A CN200410020986A CN1305388C CN 1305388 C CN1305388 C CN 1305388C CN B2004100209865 A CNB2004100209865 A CN B2004100209865A CN 200410020986 A CN200410020986 A CN 200410020986A CN 1305388 C CN1305388 C CN 1305388C
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tea beverage
tea
nanometer sod
sod
nanometer
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CN1596684A (en
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王成余
王刚
王强
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王成余
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Abstract

The present invention relates to tea beverage containing nanometer SOD and a preparation method thereof. After the tea beverage is prepared, 500 to 2000 active units of nanometer SOD is added into every kilogram of the tea beverage, and is sufficiently and uniformly stirred. The present invention has the advantages of rich nutrition, little investment, fast effect and low cost. The tea beverage containing nanometer SOD can lighten the load of the liver, protect liver cell membranes, resist the damage functions of chemical substances to liver and brain cells, inhibit inflammatory media from being generated and inflammatory reaction, sufficiently show human bodies' defense functions, regulate physiological rhythm, prevent diseases, promote rehabilitation functions, resist fatigue, enhance immunity, improve taste, promote appetite, the eubolism of vitamin A, functions of vision, and intelligence development, and has curative effects on diseases of the respiratory tract, the oral cavity, pharyngitis, upper respiratory tract infection, the intestinal tract, etc. The tea beverage into which nanometer SOD is added, can play double actions, namely the prevention of the erosion of oxygen free radicals to DNA and the removal of excess oxygen free radicals. Tea polyphenol plays the function of repairing eroded DNA. Because the nanometer SOD also has antioxidation, no preservatives need to be added, and the shelf life is prolonged by one third.

Description

Contain tea beverage of nanometer SOD and preparation method thereof
Affiliated field
The present invention relates to superoxide dismutase SOD drinks, particularly contain tea beverage of nanometer SOD and preparation method thereof.
Background technology
Human body depends primarily on and keeps basic metabolism the needs of energy; Do manual work, activity; Specific dynamic action and growing and other special requirements.Protein is the very complicated class organic compound of chemical constitution, is people's essential nutrients, and all animal and plant foods and life entity all contain protein, and this is first important substance in the vital movement, is the material base of life, does not have life without protein.Protein function is summarized and mainly contained the following aspects: protein is the indispensable constituent of tissue; Various important physical active materials in the protein constituting body; Protein participates in keeping the stable and adaptation to external world of environment in the human body; The portion of energy that protein provides body to need.
Tealeaves is the bud-leaf of plant of theaceae tea.Tealeaves has the refresh oneself, and relieving thirst and restlessness is reduced phlegm, help digestion, diuresis, detoxication, be used for headache, blurred vision, sleep more kind sleep, diseases such as restlessness and thirst, dyspepsia phlegm stagnate, malaria, dysentery.China is the native place of class leaf, and the kind of tealeaves is a lot of, can be divided into black tea, green tea, yellow tea, blue or green tea, black tea and white tea 6 big classes.Dailyly drink more have green tea, black tea, oolong tea and jasmine tea and minority area and drink more compressed tea (comprising Tuo tea, Pu'er tea, brick tea etc.).Because the place of production and processing, concocting method difference, various tea sets have different qualities and special local flavor.China utilizes tea health-care to have history in several thousand.Tealeaves contains hundreds of kind chemistry and is dividing, and removes known nutrient protein, fat, sugar, vitamin (B 1, B 2, PP, C, E and carrotene), outside mineral matter and the trace element, also contain tea polyphenolic compounds, caffeine, tannic acid, glycosides displayed, catechin, aromatic compound etc.Complicated chemical composition makes tea not only have the healthy beverage of slaking thirst and help produce saliva, and have produce refreshing effect to the mind, eliminate fatigue, help digestion, separate greasy, viral infection resisting, multiple efficacies such as anti-inflammation.Contained caffeine can promote the blood of human body circulation in the tealeaves, stimulating central nervous system, and the cardiac stimulant diuresis, the meta-alkalescence of tea helps to regulate acid-base balance.In recent years find that Tea Polyphenols can reduce blood fat and blood pressure, prevention of arterial is atherosis and thrombosis, help to prevent and treat disease of cardiovascular system.Have tea and to give protection against cancer, certain effect is all arranged for the generation of liver cancer, the cancer of the esophagus and cutaneum carcinoma.The contained tannic acid of tealeaves has astriction; The micronutrient cellulose content is variant because of the place of production and kind in the tealeaves, and it is many to contain manganese as green tea, and Dragon Well tea contains potassium, magnesium is many, and it is higher that black tea contains selenium, and carrotene and vitamin C are higher than his kind of base in the jasmine tea; Tealeaves is fluorine-containing, helps to safeguard bone, tooth health, caries prevention and osteoporosis, and some other composition also is that the body-care diseases prevention is necessary.Functional components in the tealeaves is subjected to the influence of environment temperature, pressure, air, pH value and reaction medium in the leaching process and changes.Conclusion is got up, and comprises following four kinds of variations: oxidation, hydrolysis, isomerization and other variations.
Tea beverage (pure tea juice) sugar-free, low heat value, low sodium have the quality style of stimulation, tasty and refreshing, light, glycol, are natural drink, meet the trend of modern beverage.Tea has the effect that stimulates oral mucosa, can promote the production of body fluid to quench thirst, and makes the oral cavity salubrious, and controls heat center internally and come regulate body temperature, reaches the effect of really quenching the thirst.Tea beverage is to grow up the seventies in 20th century, directly utilizes millet paste, instant tea or concentrated tea to convert water and processes, and often occurs with canned, bottled, cartridge carton form, carries, drinks conveniently, and changes in temperature are suitable.At present in the world tea beverage output more than 7Mt, consumption per head rises, West Europe 3.48L/ (people a) (1996), U.S. 7.87L/ (people a) (1994), China 19L/ of Taiwan Province (people a) (1996), Japanese 27L/ (people is (1996) a), tea beverage in the world sales volume above 10,000,000,000 dollars.In recent years, the tea beverage of China also had development, successfully developed tea beverage processing technology, had solved variable color in the tea beverage, fragrant low, precipitation Three Difficult Issues.
Yet the tealeaves extractive technique is perfect inadequately, and the composition of interpolation is also undesirable, and is not suitable for the sportsman and old man's beverage is drunk, and can not be absorbed rapidly drinking the back, can not eliminate fatigue as soon as possible, strengthens endurance, keeps the balance that blood sugar and power and water in the body are separated matter; Can not satisfy the needs of normal person and the good health and a long life of special population class, demand urgently improving.
Summary of the invention
The present invention be directed to the prior art deficiency, a kind of tea beverage that contains nanometer SOD and preparation method thereof is provided, make it to emphasize that its composition fully shows the industrialization food of body defense function, adjusting circadian rhythm, prevent disease and promotion recovery function to people's physical efficiency; And antifatigue, raising immunity; Improve the sense of taste and promote appetite; Promote the eubolism and the visual performance of vitamin A; The normal function of protection skin and bone; Promote intelligence development, satisfy the needs of human health longevity.
The tea beverage that contains nanometer SOD, nanometer SOD 500-2000 active unit/per kilogram tea beverage.
The tea beverage preparation method who contains nanometer SOD makes the back at tea beverage and adds nanometer SOD 500-2000 active unit by the per kilogram tea beverage, stirs.
New and high technology is to set up the support of tealeaves innovation industry.Antioxidant in the diet for a long time extremely Chinese scholars pay close attention to, this be because: the antioxidant in the food can be protected food self, in order to avoid things oxidation damaged and going bad; In human body alimentary canal, have antioxidation, prevent alimentary canal generation oxidative damage; Can be played a role at other histoorgans of body by the antioxidant that body absorbs; Some antioxidant in the food can be used as plant extract or purifying thing, as the treatment medicine.Antioxidation can be divided into direct effect and indirectly-acting.The chemical reaction process that antioxidant is participated in deactivation free radical or the initiation of blocking-up free radical directly is called direct effect; In contrast, play the antioxidant of indirectly-acting and do not participate in above-mentioned reaction directly, but can strengthen the oxidation resistance of cell, stop oxidative stress by number of mechanisms.
Require and domestic and international Tea Processing scientific research basis according to new and high technology innovation industry, tealeaves innovation industry can be considered from following several respects: with zymotechnic, hyperfiltration technique, supercritical CO 2Technology etc. are extracted the monomer of effective content of tea, and produce concentrate, instant tea etc., improve the quality, reduce cost; Applied Biotechnology is cultivated the tea tree cell, extracts secondary metabolites, and maximum ground improves primary product purity, improves the ratio and the content of ester catechin; Applied Computer Techniques is realized the process automatic control.At present, be developed into the process technology that simmers tea of full-automatic computer operation, realized full-automatic computer control; The development interdisciplinary science, the secret of exploration tea and health, the product that exploitation makes new advances, making tea is that health is made bigger contribution.
Nanometer SOD has utilized new and high technology means such as modern biology and physics.Typically refer to and in 0.1-100 nanometer bulk, handle atom and molecule, SOD is studied or processes, thus the new product that preparation has specific function.When a kind of material constantly is cut to a certain degree, its particle is little to be nano material to nanometer scale, and nano material tends to produce some new physicochemical properties; Nanometer technology is applied to the medicine food technical elements, has overcome an insurmountable difficult problem on some conventional medicament food and the technology, provide life for promoting medicine food to carry in vivo etc.; Can remove the excessive ultra-oxygen anion free radical of generation in the body metabolic process, delay, as the appearance of delaying decrepitude of skin and lipofuscin precipitation owing to free radical is encroached on the aging phenomenon that occurs; Improve the resistance of human body, comprise tumour, inflammation, pulmonary emphysema, cataract and autoimmune disease etc. inducing an illness owing to the free radical infringement; Improve the resistance of human body,, strengthen the body adaptive faculty of environment to external world as smog, radiation, toxic chemical and poisonous pharmaceuticals etc. to the extraneous incitant of free radical; Pain and the serious adverse of ameliorate tumor patient when carrying out chemotherapy, radiotherapy is as bone marrow injury worker leucocyte minimizing etc.; Eliminate organism fatigue, strengthen adaptive faculty the excess load large amount of exercise.
Research thinks that effect and its raising antioxidant ability of organism of tealeaves anti-cancer is closely related.Antioxidant content in the tealeaves mainly is the Tea Polyphenols that accounts for dry weight 15%-35%, the antioxidant effect of Tea Polyphenols is 9.6 times of vitamin E, 2.6 times of BHA, antioxidation activity than vitamin C and BHT is also high, kind surplus in the of about 30, comprise the depside of catechin, flavones and derivative thereof, dark brownish green plain class, phenolic acid, wherein catechin accounts for 80% of total amount.Tea Polyphenols is harmless, the good natural additive of security.Tea Polyphenols acute toxicity, subacute toxicity, subchronic toxicity, Salmonella reversion test, anti-sudden change were carried out many researchs with toxicology contents such as anticancer change test, micronucleus tests both at home and abroad, its result shows that Tea Polyphenols meets the toxicology and the safety evaluation requirement of food additives fully.The anti-oxidant usefulness of Tea Polyphenols is equivalent to copper-zinc superoxide dismutase, and both have been in conjunction with having played complementation, can strengthen anti-oxidant and reparation by the DNA of free-radical oxidation, and it is inhibited to the DNA biosynthesis of tumour cell SR-1.Tealeaves green coffee alkali content is the highest, is present in young sprout more, accounts for the 1%-5% of dry matter; Water-soluble polysaccharide in the tealeaves mainly is cellulose, starch and pectic substance, accounts for the 20%-30% of bright leaf dry weight; Chlorophyll is the main component of tealeaves marennin, accounts for the 0.3%-0.8% of bright leaf dry weight, is made up of glaucous chlorophyll a and yellowish green chlorophyll b; Colored component in the tealeaves also has carrotene, lutein, xanthin, anthocyanidin, Quercetin etc. except that chlorophyll; Xanthin, anthocyanidin all belong to the flavonoids in the phenol derivatives, are water colo(u)r.
SOD has been applied to fields such as medicine, food, health products, cosmetics as broad-spectrum biology enzyme; Lack influence, the direct absorption difference of outer time spent skin of (half-life has only a few minutes), oral digestive juice and itself be that a kind of protein has certain immunogenicity etc. greatly by (greater than 30000), half-life owing to overcome the SOD molecular weight for nanometer SOD, application is very restricted, the application of nanometer SOD in tea beverage has the effect that strengthens cellular immune function, macrophage phagocytic function and humoral immune function.Active oxygen is the important participant of tea beverage oxidation, adds nanometer SOD and can prevent the tea beverage oxidation, antioxidant ability of organism is doubled and redoubled, and makes tea beverage fresh delicious.
We use free radical biology, free radical medical science, traditional Chinese medicine and nutrition, in tea beverage, add the antioxidant nanometer SOD, and the extract of edible plant and antioxidant vitamins are main complexing agent, become the nanometer SOD tea beverage, through a series of experiments, prove that it is a kind of fresh efficiently health food.The nanometer SOD tea beverage is a kind of new function food, and the permeability in the human small intestine greatly improves; Rate of release improves; Effect and effective rate of utilization improve greatly, detect data according to contrasting, nearly 10 times of active raising.Nanometer SOD can effectively be broken through the restriction of body biological barrier to the effect of medicine food, makes medicine food enter blood circulation better.Human body has many protection bodies, is the natural biological barrier, as blood-brain barrier; blood-eye barrier, cell biological envelope barrier etc., general medicine is difficult to the biological barrier by these bodies; the nanometer SOD particulate then may pass these barriers, and lesions position is played therapeutic action.
Nanometer SOD has effects such as the color spot of removing, chloasma, wrinkle to Ms's skin, and can increase the elasticity of skin membrane, prevents aging; The man there is the fatigue of elimination, eliminates inferior health, improves antiviral ability, prevent aging, and can reduce the prostatitic incidence of disease.This technology has overcome the application restriction and the deficiency of bio proteases such as present SOD, has opened new gate for its extensive use, is suitable for penetration and promotion.
Testing 1 result shows: the SOD of oral uptake can enter in the body.Give mouse stomach with SOD, reach the top behind the 3h in blood, most of SOD is distributed in the blood plasma, and fraction enters in the haemocyte, and the distribution sequence of SOD in tissue is kidney>liver>spleen>heart.
Testing 2 results shows: tried in the thing SOD group and nanometer SOD group mouse swimming time and negative control group relatively, and the SOD obvious prolongation of group (P<0.05), the average swimming time of nanometer SOD group is significant prolongation (P<0.001) then.According to the antifatigue effect method of inspection, the kinds of processes formulation of SOD has been carried out the swimming with a load attached to the body test, result of the test is positive, show that two kinds of SOD all have anti-fatigue effect, and the antifatigue effect of nanometer SOD is more obvious.
Testing 3 results shows: the body weight test, and each dosage treated animal body weight sustainable growth is compared with negative control group, and sod organizes no significant difference, and nanometer sod organizes significant difference (p<0.05).
Conclude the tea beverage that gets up to contain nanometer SOD and have following characteristics: be nutritious, delicious, various in style; Produce simple, invest little, instant effect, cost is low, is easy to popularization; Raw material sources are extensive, abundant sufficient; Can alleviate load of liver, the protection liver plasma membrane, the opposing chemical substance is to hepatocellular damaging action; The opposing chemical substance suppresses inflammatory mediator simultaneously again and produces the damaging action of brain cell, the inflammation-inhibiting reaction; Effective in cure to respiratory tract, oral cavity, pharyngitis and last sense and enteron aisle etc., and antifatigue, raising immunity, the tea beverage that adds nanometer SOD, it can play double action, prevents that oxygen radical from corroding DNA, removes unnecessary oxygen radical, Tea Polyphenols plays the effect that is etched DNA of repairing, because of nanometer SOD has antisepsis, do not add any anticorrisive agent shelf-life raising 1/3, can satisfy the needs of human health longevity.
The specific embodiment:
The tea beverage that contains nanometer SOD, nanometer SOD 1000 active units/per kilogram tea beverage.
The tea beverage preparation method who contains nanometer SOD below 40 ℃ the time, makes the back at tea beverage and adds nanometer SOD 1000 active units by the per kilogram tea beverage, stirs.
Example 1, tea (green tea, black tea, oolong tea, yellow tea) add water-cooled and extract; The 350ml tea beverage adds nanometer SOD 200 active units.Human body absorbed nanometer SOD 6000 active units in one day at most.To respiratory tract, oral cavity, pharyngitis and to go up sense and enteron aisle etc. effective in cure, and antifatigue, raising immunity, the shelf-life improves 1/3.
Example 2, green tea powder 2.5g, white granulated sugar 7.5g, sorbierite 7.5g, sodium ascorbate 1g, water 100mL, nanometer SOD 100 active units.The green tea of nanometer SOD end method for preparing beverage except that nanometer SOD, stirs whole raw materials, carries out high-temperature short-time sterilization (106-116 ℃ 2s), below 40 ℃ the time, adds nanometer SOD and aseptic packaging.Packaging material are generally carton or metal can.This beverage is not owing to pass through long-time high-temperature process, and nutrition does not have destroyed, can keep distinctive green of green tea and delicate fragrance for a long time, and embodies the effect of nanometer SOD.
Example 3, contain the tea beverage preparation method of nanometer SOD, water → processing → purifying → adding tealeaves (black tea, green tea, oolong tea, jasmine tea etc.) and heating → extraction → cooling → filtration → allotment → heating → sterilization → cooling → nanometer SOD → canned.
Example 4, contain the cold drink instant tea method for preparing beverage of nanometer SOD, the quality of tea will have fragrance, astringent taste and color and luster.When leaching, can keep its good fragrance and color and luster, but can not obtain astringent taste with cold water; When leaching with hot water, though can obtain astringent taste, its fragrance can vapor away in leaching process, for both get both, can adopt following method preparation:
(1), tealeaves put into contain the aqueous solution that cyclodextrine is the heavy 1-10% of tea, abundant dipping back squeeze and filter under 20-40 ℃ of temperature adds ascorbic acid and excipient again, is cooled to 8 ℃ of preservations, is leachate (a).
(2), the tea leaf residual after pressing is with 85-90 ℃ the airtight dipping of hot water, cooling back squeeze and filter adds ascorbic acid and excipient in leachate, be leachate (b).
(3), (b) liquid residue is carried out once identical leaching process again, leachate (c).
(4), carry out vacuum after (b), (c) liquid merged and concentrate, concentrate remerges (a) liquid, through centrifugation, must clarify extract, adds nanometer SOD in the time of below 40 ℃, with spraying or other method is carried out drying.
The quality management of tea beverage: food GMP is a kind of concrete quality guarantee system, its aim is to make food factory in processes such as manufacturing, packing and storing, settings such as the relevant personnel, building, facility and health, manufacture process, quality management all can meet good working condition, prevent food unhygienic conditioned disjunction may cause pollute and environment that quality degenerates in operate, reduce the generation of production accident, guarantee the stable of food safety and sanitation and quality.After the U.S., the GMP system is also being carried out as Japan, Canada, Singapore, Germany, Australia, China etc. in many countries and regions.

Claims (6)

1, the tea beverage that contains nanometer SOD is characterized in that nanometer SOD 500-2000 active unit/per kilogram tea beverage.
2, according to the described tea beverage that contains nanometer SOD of claim 1, it is characterized in that nanometer SOD 1000 active units/per kilogram tea beverage.
3,, it is characterized in that green tea powder 2.5g, white granulated sugar 7.5g, sorbierite 7.5g, sodium ascorbate 1g, water 100mL and nanometer SOD 100 active units according to the described tea beverage that contains nanometer SOD of claim 1.
4, the tea beverage preparation method who contains nanometer SOD makes the back at tea beverage and adds nanometer SOD 500-2000 active unit by the per kilogram tea beverage, stirs.
5,, it is characterized in that below 40 ℃ the time tea beverage is made the back and added nanometer SOD 1000 active units by the per kilogram tea beverage, stirs according to the described tea beverage preparation method who contains nanometer SOD of claim 4.
6, according to the described tea beverage preparation method who contains nanometer S0D of claim 4, it is characterized in that green tea powder 2.5g, white granulated sugar 7.5g, sorbierite 7.5g, sodium ascorbate 1g, water 100mL, nanometer SOD 100 active units; Except that nanometer SOD, whole raw materials are stirred, carry out high temperature 106-116 ℃, the 2s instantaneous sterilizing below 40 ℃ the time, adds nanometer SOD and aseptic packaging.
CNB2004100209865A 2004-07-19 2004-07-19 Tea beverage containing nanometer SOD and its preparation method Expired - Fee Related CN1305388C (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101700212B (en) * 2009-07-23 2012-04-18 杭州六易科技有限公司 Method for making anti-aging moisturizer
CN103609790B (en) * 2013-10-31 2015-04-15 安徽博格生物科技有限公司 Tea bag capable of enhancing immunization and preventing disease

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1214211A (en) * 1997-10-13 1999-04-21 穆运转 Anti-senility cancer-preventing health beverage
CN1483337A (en) * 2003-05-01 2004-03-24 李中华 SOD tea leaf and productive method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1214211A (en) * 1997-10-13 1999-04-21 穆运转 Anti-senility cancer-preventing health beverage
CN1483337A (en) * 2003-05-01 2004-03-24 李中华 SOD tea leaf and productive method thereof

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