CN1302100C - Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma - Google Patents
Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma Download PDFInfo
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- CN1302100C CN1302100C CNB2004100281764A CN200410028176A CN1302100C CN 1302100 C CN1302100 C CN 1302100C CN B2004100281764 A CNB2004100281764 A CN B2004100281764A CN 200410028176 A CN200410028176 A CN 200410028176A CN 1302100 C CN1302100 C CN 1302100C
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- glossy ganoderma
- disinfectant
- ganoderma
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Abstract
The present invention discloses an application of using chlorine dioxide as disinfecting agents for cultivating glossy ganoderma. The products have wide sterilizing boards, strong disinfecting effect, no harm to people and livestock, and no influence to the growth of (ganoderma lucidum)As. 5.65 and 7 types of the glossy ganoderma. Culturing mediums are needless to disinfect and can be used for the separation of glossy ganoderma fruit bodies, strain expanding cultivation, the fermentation of glossy ganoderma mycelia and glossy ganoderma polysaccharide and the solid culture of the glossy ganoderma only by added with right quantity of the disinfecting agents. The present invention greatly simplifies glossy ganoderma culturing productive processes, saves energy source and obviously reduces productive cost. The disinfecting agents have the advantages of low price and low cost and can be widely used for sterilizing strain culturing mediums, such as the glossy ganoderma, etc.
Description
Technical field
The present invention relates to dioxide peroxide (ClO
2) purposes, relate in particular to the purposes of dioxide peroxide as disinfectant.
Background technology
Dioxide peroxide is a strong oxidizer; Can strong oxidizing reaction take place with phenols, sulphur class, tertiary amine, organic microbial, metal ion etc.; React slightly with alkanes, alkene class, alcohols, aldehydes, ester class, organic acid etc., can not produce chlorophenol, stink substance and carcinogenic substances such as trichloromethane, it can not produce secondary pollution to environment.The late nineteen eighties; dioxide peroxide is as food sanitizer; drinking water disinfect; obtained the approval of USDA and USEPA; world health office thinks that this material does not have carinogenicity, teratogenesis shape fully; it is come safe disinfection agent first place, is widely used in sterilization, sterilization, anticorrosion, deodorizing, field such as fresh-keeping, be described as the 4th generation disinfectant.
In traditional glossy ganoderma was cultivated, prepared culture medium all needed all will take strict aseptic manipulation through high-temperature sterilization in inoculation and culturing process, and glossy ganoderma is cultivated cost height, technical requirements height.
Summary of the invention
The object of the present invention is to provide the agent of a kind of glossy ganoderma medium sterilization.
The present invention uses dioxide peroxide as the agent of glossy ganoderma medium sterilization.
Specifically, dioxide peroxide is to add the dioxide peroxide of 7～10mL in every 1000mL liquid medium as the interpolation concentration of glossy ganoderma medium sterilization agent.
The present invention adopts dioxide peroxide to be applied to the glossy ganoderma substratum as disinfectant, and fungicidal spectrum is wide, and sterilizing power is strong, to the person poultry harmless, but to red sesame (Ganoderma lucidum) As.5.65 and 7 kinds of not influences of glossy ganoderma growth.Experimental study shows: substratum does not need sterilization, as long as add the fermentation that an amount of disinfectant just can be used for Ganoderma sporophore separation, strain expanded culture and Ganoderma mycelium, ganoderan, and the solid state cultivation of glossy ganoderma, simplify the production process that glossy ganoderma is cultivated greatly, saved the energy, significantly reduced production cost.This disinfectant low price, cost is low, but wide popularization and application is in the sterilization of bacterium culture mediums such as glossy ganoderma.
In order to understand essence of the present invention better, will its unusual effect in the sterilization field of glossy ganoderma substratum be described with experimental data below.
1. disinfectant is to the restraining effect experiment of mould and bacterium
The dioxide peroxide of employing 0.1%～1.0% compares experiment as the substratum of disinfectant and the control group of sterilized, and incubation time is 5 days.
Table 1 disinfectant is to the restraining effect of mould and bacterium
| 0.9% 1.0% contrasts | --cover with | --cover with | --cover with | --cover with |
Annotate :+mycelial growth arranged; ++ mycelial growth is many; +++mycelial growth is a lot;-no mycelia growth
Analyzed by table 1 and to learn: control tube all covers with assorted bacterium within 5 days, and has added all long bacterium of substratum of 0.7% above disinfectant, and adds the growth that 0.1% disinfectant just can suppress head mold and each bacterioid fully; Aspergillus niger and mould are also increased by the inhibition degree thereupon along with the increase of disinfectant concentration, when disinfectant concentration is increased to 0.7%, can suppress the growth of aspergillus niger and mould fully.Therefore, when selecting the concentration of disinfectant for use, do not answer the end in 0.7%.
2. the different concns disinfectant is to the restraining effect experiment of red sesame
Adopt 0.1%～5.0% dioxide peroxide to compare experiment as the substratum of disinfectant and control group through high-temperature sterilization, incubation time is 10～12 days.
Under the different disinfectant concentration of table 2 to the growing state of red sesame
| 0.3% 0.4% 0.5% 0.6% 0.7% 0.8% 0.9% 1.0% 2.0% 3.0% 4.0% 5.0% contrasts | Sprout-----------sprout | + sprout---++ | +++ ++ ++ ++ ++ ++ ++ ++ + - - - +++ | Cover with +++---cover with |
Annotate :-no mycelia growth; + mycelial growth arranged; ++ mycelial growth is many; +++mycelial growth is a lot
Got by table 2: disinfectant concentration begins that at 0.4% o'clock red sesame is had certain restraining effect, make red sesame sprout than control group late 2 days, but the time of covering with is roughly the same, observation after the 12nd day, all overgrow with, promptly (0.1%-1.0%) disinfectant is little to the growth effect of red sesame.Disinfectant concentration is at 2.0% o'clock, and to the sprouting of glossy ganoderma with cover with all influentially, promptly 2.0% disinfectant has restraining effect to glossy ganoderma; Disinfectant concentration began to suppress fully the growth of red sesame at 3.0% o'clock.
Associative list 1: when cultivating red sesame, select for use the concentration of disinfectant to be preferably in (0.7%-1.0%), this concentration can play good restraining to mould, and little to red sesame growth effect.
3. disinfectant is to the influence experiment of other several glossy ganoderma growths
The dioxide peroxide of employing 0.7% compares experiment as the substratum and the control group through sterilizing of disinfectant, and incubation time is 10～12 days.
Table 3 0.7% disinfectant is to the influence of other several glossy ganoderma growths
Annotate: contrasting is that the substratum process is sterilized, and all the other are all unsterilised;-no mycelia growth; + mycelial growth arranged; ++ mycelial growth is many; +++mycelial growth is a lot
Get by table 3: the equal normal growth of the Ganderma lucidum of control group.And added the U.S. glossy ganoderma of 0.7% disinfectant, Japanese glossy ganoderma, red sesame, black sesame, Huizhou glossy ganoderma, Xinzhou glossy ganoderma) with compare, some glossy ganoderma sprout time is postponed slightly, but cover with the time and control group is basic identical, illustrate that 0.7% disinfectant is little to the influence of glossy ganoderma class.But this disinfectant has the obvious suppression effect for purple sesame.
4. disinfectant transformation period experiment
Shake bottle observed in 12 days: 2 bottles of comprehensive liquid nutrient medium clarifications of the PDA through sterilization, all there are not phenomenons such as muddiness.And 5 bottles added the comprehensive liquid nutrient medium of PDA of 0.7% disinfectant without high-temperature sterilization, also is clarification, phenomenons such as no muddiness.The transformation period that this disinfectant is described is longer, generally need not to add once more disinfectant and prevent microbiological contamination in culturing process.
5. disinfectant is to the influence experiment of glossy ganoderma shake-flask culture
Bottled substratum 100ml of 250ml triangle and the bottled substratum 1000ml of 3000ml triangle shake-flask culture are observed: experimental group (0.7% disinfectant is unsterilised) and control group (sterilising medium) all had the mycelium germination sign in the 2nd day, the 8th day, the white hypha ball all covers with bottle, and the two difference of mycelia dry weight is little.Above-mentioned experiment repeats repeatedly, illustrates that this disinfectant has certain practical value to the deep layer cultivation of glossy ganoderma.
6. disinfectant is to the influence experiment of cultivation of glossy ganoderma
Conventional material cultivation of glossy ganoderma: adding 0.7% disinfectant is unsterilised in the solid material, and the 4th day glossy ganoderma begins to sprout, and after the 20th day, material all covers with mycelia, and does not have the microbiological contamination phenomenon, and promptly mycelia is white, does not have other colors, no unpleasant taste in the bag.Ganoderma sporophore and contrast difference are little.
Embodiment
Now with the application of specific embodiment explanation dioxide peroxide disinfectant in the glossy ganoderma substratum.
Prepare each substratum with following material:
1. slant medium
PDA synthetic medium: potato (peeling) 200g, glucose 20g, KH
2PO
40.75g, MgSO
40.3g, agar 20g, water 1000ml.And in water, add liquid dioxide peroxide 7ml.
2. shake-flask seed substratum
PDA synthetic medium: potato (peeling) 200g, glucose 20g, KH
2PO
40.75g, MgSO
40.3g, water 1000ml.And in water, add liquid dioxide peroxide 7ml.
3. shake flask fermentation substratum
Analysis for soybean powder synthetic medium: analysis for soybean powder 20g, glucose 20g, yeast extract paste 2g, KH
2PO
40.75g, MgSO
40.3g, water 1000ml.And in water, add liquid dioxide peroxide 7ml.
4. solid state cultivation substratum
Wheat bran 96.3%, sucrose 1%, gypsum (CaSO
4) 1%, calcium superphosphate 1%, water adds 1000ml by each kilogram solid materials, and adds liquid dioxide peroxide 7ml in water.
Claims (2)
1. use the method for dioxide peroxide as the agent of glossy ganoderma medium sterilization.
2. application dioxide peroxide according to claim 1 is characterized in that adding in every 1000mL liquid medium the liquid dioxide peroxide of 7～10mL as the method for glossy ganoderma medium sterilization agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100281764A CN1302100C (en) | 2004-07-22 | 2004-07-22 | Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100281764A CN1302100C (en) | 2004-07-22 | 2004-07-22 | Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1597924A CN1597924A (en) | 2005-03-23 |
| CN1302100C true CN1302100C (en) | 2007-02-28 |
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ID=34664172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100281764A Expired - Fee Related CN1302100C (en) | 2004-07-22 | 2004-07-22 | Application of using chlorine dioxide as disinfecting agent for cultivating glossy ganoderma |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960179A (en) * | 2011-08-29 | 2013-03-13 | 何寒 | Process for manufacturing liquid strain by using raw culture medium |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080206215A1 (en) * | 2006-02-22 | 2008-08-28 | Allen Michael Ziegler | Apparatus and method for treatment of microorganisms during propagation, conditioning and fermentation |
| CN102580129A (en) * | 2012-02-25 | 2012-07-18 | 何寒 | High-pressure-free and short-term normal pressure sterilizing method for cordyceps sinensis culture medium |
| CN102630493B (en) * | 2012-04-26 | 2014-03-12 | 刘德育 | Method and equipment for preparing liquid strain of edible and medicinal fungi by using conventional drinking water |
| CN103392499A (en) * | 2013-06-22 | 2013-11-20 | 何寒 | Method for preparing edible fungus fruiting fungus bars from full raw materials directly |
| CN105363052A (en) * | 2015-12-10 | 2016-03-02 | 何寒 | Quick sterilization method of liquid culture medium for edible fungi |
| CN105886430A (en) * | 2016-04-27 | 2016-08-24 | 石家庄大众肥业有限公司 | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation |
| CN106171970B (en) * | 2016-06-20 | 2018-06-15 | 淮北师范大学 | Rapid preparation method of plant culture medium with low phenol content |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1295795A (en) * | 2000-10-31 | 2001-05-23 | 济南化肥厂有限责任公司 | Agricultural disinfectant |
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2004
- 2004-07-22 CN CNB2004100281764A patent/CN1302100C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1295795A (en) * | 2000-10-31 | 2001-05-23 | 济南化肥厂有限责任公司 | Agricultural disinfectant |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960179A (en) * | 2011-08-29 | 2013-03-13 | 何寒 | Process for manufacturing liquid strain by using raw culture medium |
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| Publication number | Publication date |
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| CN1597924A (en) | 2005-03-23 |
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