CN102960179A

CN102960179A - Process for manufacturing liquid strain by using raw culture medium

Info

Publication number: CN102960179A
Application number: CN2011102631654A
Authority: CN
Inventors: 何寒
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-08-29
Filing date: 2011-08-29
Publication date: 2013-03-13

Abstract

The invention belongs to the field of deep fermentation of edible fungi liquid strain, and particularly relates to a process for manufacturing liquid strain by using raw culture medium. According to the process, the liquid culture medium is sterilized by using chlorine dioxide as a sanitizer, and can not be subjected to high-temperature and high-pressure or atmospheric sterilization. The well sterilized liquid culture medium is joined up with the edible fungi strain, and then the edible fungi liquid strain can be obtained through conventional culture. In the implementation, not only does no energy consumption exist, but also complicated procedures can be reduced, nutrient in the liquid culture medium can not be lost due to high-temperature and high-pressure sterilization. Through the technical measures, massive, high-quality and low-cost liquid strains can be produced easily.

Description

A kind of technique of making liquid spawn of the raw material medium

Technical field

The invention belongs to biological submerged fermentation technical field, be specifically related to a kind of technique of making liquid spawn of the raw material medium.

Background technology

The edible fungus solid spawn production cycle is long, cost is high, easy pollution, have a big risk, and that edible fungi liquid strain has is with short production cycle, cell age neat and consistent, inoculation are convenient, send out bacterium fast, be suitable for the advantage such as batch production production, its China's Edible Fungi that thoroughly is through with has been continued to use the history that more than one thousand years adopts solid spawn, is a qualitative leap.

Liquid spawn produce be use biological submerged fermentation engineering preparation and highly purified bacterial classification.Its birth will be eliminated traditional workshop-based production of employing solid spawn huge numbers of families, and from then on peasant household's kind mushroom is got twice the result with half the effort, and batch production, standardized production have had guarantee.

Traditional liquid spawn in process of production, liquid culture matrix must be through strict autoclave sterilization or for a long time normal pressure sterilization, not only consume thus a large amount of energy, also so that technique becomes loaded down with trivial details, complicated, the nutrient of liquid nutrient medium also can be because causing running off to some extent through autoclave sterilization.

Number of patent application is 01114001.1 " edible fungi liquid strain production method and equipment ", the fermentation tank that edible fungus liquid culture growth medium matter also needs to involve great expense is as autoclave sterilization container and culture vessel, and this method makes the production cost of liquid spawn higher.

Number of patent application is 200410021441.6 " technology for producing ediable mushroom with decanned liquid bacterial spawn ", use cheap cylindrical glass bottle or plastic bottle to enlarge the liquid spawn output, the incubator that does not need to involve great expense is as autoclave sterilization container and culture vessel, but liquid spawn culture medium matter still needs to come autoclave sterilization or for a long time normal pressure sterilization by other approach, can access bacterial classification and cultivate into liquid spawn, equally very loaded down with trivial details, the complicated and consumption mass energy of this technique.

Summary of the invention

The object of the present invention is to provide a kind of technique of making liquid spawn of the raw material medium, it has overcome traditional liquid bacterial classification production technology and has used fermentation tank, a large amount of energy of consumption that involves great expense, production technology is loaded down with trivial details, complicated, and the nutrient of liquid nutrient medium is because of shortcomings such as the process autoclave sterilization cause running off to some extent.Institute provides a kind of and utilizes chlorine dioxide to make disinfectant to realize that liquid spawn culture medium does not need through high temperature, autoclaving, thereby not only reduced loaded down with trivial details, complicated technique, also need not power consumption, the technical matters that the nutrition of liquid nutrient medium is run off to some extent.

Used chlorine dioxide among the present invention, efficient, the wide spectrum of the latest generation of generally acknowledging in the world at present, the sterilization of safety, can under extremely low concentration (0.1ppm), kill the thalline of virus, bacterium, protist, algae, fungi and various spore and sporulation, be widely used at World Developed Countries.The Ministry of Chemical Industry of country has promulgated relevant industry standard, and health ministry approved chlorine dioxide is disinfectant and new food additive.

After chlorine dioxide adds liquid spawn culture medium matter according to quantity to, in 30 minutes, just finish whole sterilizing process; Again through quiet put 30 hours after, the sterilizing ability of chlorine dioxide will complete obiteration, at this moment accesses edible fungus species, can any injury not arranged to bacterial classification.

The present invention is achieved through the following technical solutions:

1, the raw material medium is made the technique of liquid spawn, and its production technology is:

(1) select chlorine dioxide to make disinfectant.

(2) come routinely the obtaining liq medium, and in the fermented and cultured container of packing into according to quantity.

(3) after liquid nutrient medium prepared, the 0.1-0.3% that presses liquid nutrient medium added chlorine dioxide, and fermented and cultured container envelope is tight.

(4) after liquid nutrient medium is thrown in chlorine dioxide according to quantity, need quiet putting just to access later on bacterial classification in 36 hours.

(5) liquid nutrient medium is cultivated routinely and is obtained edible fungi liquid strain after gnotobasis is lowered to bacterial classification.

Compared with prior art, the present invention has the following advantages:

1, the present invention select disinfectant---chlorine dioxide is cheap, consumption is few during use;

2, use the present invention can realize that liquid spawn culture medium does not need through high temperature, autoclaving;

3, use the present invention not only to reduce loaded down with trivial details, complicated technique, also need not power consumption, the nutrition of liquid nutrient medium is run off to some extent;

4, use that the present invention can easy to doly produce in a large number, high-quality reaches cheaply liquid spawn.

Embodiment

Below in conjunction with embodiment method of the present invention is further specified.

(1) select chlorine dioxide to make disinfectant.

Claims

1. make the technique of liquid spawn with the raw material medium for one kind, it is characterized in that:

(1) select chlorine dioxide to make disinfectant.