CN106119289A - A kind of composite flora Synergistic degradation stalk cellulose and the combined pretreatment method of fermentation and hydrogen production - Google Patents
A kind of composite flora Synergistic degradation stalk cellulose and the combined pretreatment method of fermentation and hydrogen production Download PDFInfo
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Abstract
The present invention relates to a kind of composite flora Synergistic degradation stalk cellulose and the combined pretreatment method of fermentation and hydrogen production, belong to field of solid waste treating technology.The method utilizes composite flora combining hydrogen oxidation sodium and acidifying steam explosion combined pretreatment method that stalk fibrid element is carried out efficient anaerobic fermentation bio-hydrogen first.First powder of straw is broken into the powder of 30～60 mesh, then carries out NaOH pretreatment, finally carry out being acidified steam explosion pretreatment and obtain pretreated straw powder, add composite flora, be placed in batch fermentation and produce cultivation in hydrogen assay device, carry out efficient anaerobic fermentation bio-hydrogen.The present invention, by sodium hydroxide and acidifying steam explosion combined pretreatment, improves product hydrogen rate and the cellulose degradation rate of Hydrogen Production in Straw Fermentation, it is achieved the resource of a large amount of straws and energy.
Description
Technical field
The invention belongs to field of solid waste treating technology, particularly to one, wastes straw is first carried out associating in advance
Process, then composite flora Synergistic degradation cellulose and under anaerobic fermentation conditions, the method for biological hydrogen production.
Background technology
Straw is that a class is widely distributed, the lignocellulosic material that reserves are huge, be also a kind of abundant and cheap can be again
Production-goods source.But for want of its effective utilization is caused the huge waste of resource at present, generally use the processing mode burned
Also serious environmental pollution is easily caused.Utilize lignocellulosic material bioconversion with corn straw as representative produce cleaning,
Efficiently, the new forms of energy-hydrogen of safety, be increasingly becoming the focus of people's research, and caused great attention both domestic and external, had
The most far-reaching economic worth and strategic importance.
Summary of the invention
The purpose of the present invention is to propose to a kind of combined pretreatment method by sodium hydroxide and acidifying steam explosion straw is entered
Row combined pretreatment, then composite flora Synergistic degradation stalk cellulose and under anaerobic fermentation conditions, the method for biological hydrogen production.
The method using the present invention, can improve product hydrogen rate and straw degradative rate, it is achieved while the resource of a large amount of straws and energy
Produce cleaning, efficient, safe new forms of energy-hydrogen.
To achieve these goals, the technical solution used in the present invention is: a kind of composite flora Synergistic degradation stalk fibre
Element and the combined pretreatment method of fermentation and hydrogen production, comprise the steps:
1) straw combined pretreatment: powder of straw is broken into powder of straw, first through NaOH solution pretreatment, more acidified steam explosion is pre-
Process, obtain combined pretreatment powder of straw.
2) anaerobic fermentation of biological produces hydrogen: batch fermentation produces hydrogen assay device deoxygenation sterilizing, then by combined pretreatment straw
Powder is soluble in water, obtains stalk cellulose substrate, is placed on agitator, and composite flora is inoculated with the ratio of 5-10%, seals and holds
Device, at 37～40 DEG C, under the conditions of impact strength is 90～130rpm, carries out cultivating and fermentation and hydrogen production, and ferment 48h, collects and produces
Gas.
Above-mentioned method, it is preferred that described powder of straw particle diameter is 30～60 mesh.
Above-mentioned method, it is preferred that described through NaOH solution pretreatment is: by solid-to-liquid ratio 1:10-15, take powder of straw and
NaOH solution, after soaking at room temperature 20-25h, is washed to neutrality；The mass percentage concentration of described NaOH solution is 0.5%-
2.0%.It is furthermore preferred that solid-to-liquid ratio is 1:10, the mass percentage concentration of NaOH solution is 1.0%.
Above-mentioned method, it is preferred that described acidified steam explosion pretreatment is: by solid-to-liquid ratio 1:10-15, the NaOH that learns from else's experience is molten
The pretreated powder of straw of liquid and aqueous sulfuric acid are placed in container, mixing, the concentration expressed in percentage by volume of described aqueous sulfuric acid
For 1-1.5%；Container is sealed, in being placed in pressure cooker, carries out steam explosion, after steam explosion completes, carry out lowering the temperature, blood pressure lowering, be cooled to room temperature
After, rinse to neutral with water, dry, obtain combined pretreatment powder of straw.It is furthermore preferred that solid-to-liquid ratio is 1:10, aqueous sulfuric acid
Concentration expressed in percentage by volume be 1%.It is furthermore preferred that steam explosion condition is: container is sealed, is placed in pressure cooker, in 120-125 DEG C,
Under pressure 0.15-0.20MPa, steam explosion 2-3h；After steam explosion completes, when pressure cooker temperature is down to 90-95 DEG C, blood pressure lowering, it is cooled to room
Temperature, is washed to neutrality, dries, obtain combined pretreatment powder of straw at 80 DEG C.
Above-mentioned method, it is preferred that described step 2) in, by soluble in water for combined pretreatment powder of straw, obtaining concentration is
The stalk cellulose substrate of 10-20g/L, inoculates composite flora by inoculum concentration 6%.
Above-mentioned method, described composite flora is that strain X 9 and bacterial strain B2 are by the mixing of 1:1.Described strain X 9
NCBI number of registration is EU434651；The NCBI number of registration of described bacterial strain B2 is EU639425.
The method have the advantages that
By straw after sodium hydroxide and acidifying steam explosion combined pretreatment, after composite flora Synergistic degradation combined pretreatment
Straw hydrogen production potential is relatively strong, and it produces hydrogen rate and straw degradative rate is all significantly improved, and not through pretreatment or only through one
In the straw of item pretreatment, the degree of crystallinity of cellulose is high, and between lignin with cell polysaccharide, close physical chemistry is combined, and limits
Composite flora cellulose degrading enzyme contacts with cell wall polysaccharides, causes the degraded of straw and the fermentation and hydrogen production process more slow
And produce hydrogen rate and degradation rate is the lowest slowly,.
The method of the present invention has obviously castering action to product hydrogen rate and straw degradative rate.The method is for using biology
Method preparation cleaning, efficient, safe new forms of energy-hydrogen provide a kind of new preprocess method, a large amount of for realizing further
Stalk resource, energy provide probability.
Accompanying drawing explanation
Fig. 1 is corn straw microscopic comparison before and after sodium hydroxide and acidifying steam explosion combined pretreatment；
In figure, before a is pretreatment, after b is pretreatment.
Fig. 2 is that a kind of batch fermentation produces hydrogen assay device schematic diagram.
In figure, 1. serum bottle；2. air bath agitator；3. air valve；4. tank；5. graduated cylinder；6. conduit.
Fig. 3-1 is sodium hydroxide and the acidifying steam explosion combined pretreatment impact on Hydrogen Production in Straw Fermentation ability.
Fig. 3-2 is the H of variable concentrations2SO4The pretreatment impact on Hydrogen Production in Straw Fermentation ability.
Fig. 3-3 is the NaOH pretreatment of the variable concentrations impact on Hydrogen Production in Straw Fermentation ability.
Fig. 3-4 is the NH of variable concentrations3·H2The O pretreatment impact on Hydrogen Production in Straw Fermentation ability.
Fig. 4-1 is the change of composite flora Synergistic degradation pretreatment corn straw reducing sugar amount.
Fig. 4-2 is the change of composite flora Synergistic degradation pretreatment cornstalk biological amount.
Detailed description of the invention
Below by specific embodiment, the present invention will be further described, but the invention is not limited in this.Due to various places
Corn straw kind is different with composition, and pretreatment parameter also can produce respective change, therefore without prejudice to the present invention essence and
On the premise of right, key parameter in the present invention can be made the appropriate adjustments.
Embodiment 1 composite flora Synergistic degradation stalk cellulose and the combined pretreatment method of fermentation and hydrogen production
Method is as follows:
1) corn straw smashing
Corn straw takes from Shenyang suburbs.Corn straw is cut into the segment of 3～5cm, then utilizes microphyte powder
These segments are ground into the powder of 30～60 mesh by broken machine, obtain powder of straw.
2) corn straw combined pretreatment
By solid-to-liquid ratio 1:10 (g/ml), take powder of straw and mass percentage concentration is the NaOH solution of 1.0%, soaking at room temperature
After 24h, it is washed to neutrality, dries at 80 DEG C.
By solid-to-liquid ratio 1:10 (g/ml), the pretreated powder of straw of NaOH solution of learning from else's experience and concentration expressed in percentage by volume are 1%
Aqueous sulfuric acid, is placed in container, mixing.Container is sealed, is placed in pressure cooker, in 121 DEG C, under pressure 0.15MPa, steam explosion
2h；After steam explosion completes, when pressure cooker temperature is down to 90 DEG C, blood pressure lowering, it is cooled to room temperature after taking-up, is washed to neutrality, 80 DEG C of drying,
Obtain through sodium hydroxide and acidifying steam explosion combined pretreatment corn stalk powder (ESCS).
3) anaerobic fermentation of biological produces hydrogen
As in figure 2 it is shown, produce hydrogen assay device for a kind of batch fermentation: serum bottle 1 is placed in air bath vibration as reactor
On device 2, the built-in water of tank 4, the opening of graduated cylinder 5 inserts in water, and conduit 6 one end connects with serum bottle 1, the other end and graduated cylinder 5
Connection.
Being placed in serum bottle 1 by combined pretreatment corn stalk powder ESCS, add water mixing, obtains the Semen Maydis that concentration is 15g/L
Stalk cellulose substrate, to serum bottle and powder of straw autoclave sterilization, and with High Purity Nitrogen (99.9%) stripping 10min to drive away
Gas phase in serum bottle and the oxygen in liquid phase, by 6% inoculum concentration, (strain X 9 and bacterial strain B2 press 1:1 (v/v) to add composite flora
Mixing.The NCBI number of registration of described strain X 9 is EU434651；The NCBI number of registration of described bacterial strain B2 is
EU639425), using rubber stopper seal container, open air valve 3, in 37 DEG C, impact strength is under 100rpm, and 48h is cultivated in concussion, produces
Raw gas, in conduit is filled with graduated cylinder, collects the gas produced.
The contrast experiment of embodiment 2 preprocess method
Method step, with embodiment 1, simply changes step 2) in preprocess method.Use device as shown in Figure 2, different
Serum bottle 1 employed in pretreated straw substrate different.Particularly as follows:
In A: embodiment 1, use sodium hydroxide and the combined pretreatment corn stalk powder of acidifying steam explosion combined pretreatment
(ESCS)。
B: sulfur acid pretreatment: dose volume percentage concentration is the H of 0.2%, 0.5%, 1.0% respectively2SO4Solution, solid-to-liquid ratio
For 1:10, soaking at room temperature 24h；Corn straw use water after process rinses to neutral, then in 80 DEG C of drying, obtains independent sulphuric acid pre-
The pretreatment corn stalk powder processed.
C: NaOH pretreatment: preparation mass percentage concentration is the NaOH solution of 0.5%, 1.0%, 2.0% respectively, Gu
Liquor ratio is 1:10, soaking at room temperature 24h；Corn straw use water after process rinses to neutral, then in 80 DEG C of drying, obtains independent hydrogen
The pretreatment corn stalk powder of sodium oxide pretreatment.
D: pretreatment with agueous Ammonia: dose volume percentage concentration is the ammonia spirit of 2.0%, 5.0%, 10.0% respectively, solid-liquid
Ratio is 1:10, soaking at room temperature 24h；Corn straw use water after process rinses to neutral, then in 80 DEG C of drying, obtains independent ammonia
The pretreatment corn stalk powder of pretreatment.
Result is as follows:
As it is shown in figure 1, corn straw microscopic comparison before and after sodium hydroxide and acidifying steam explosion combined pretreatment.By in figure
Can be seen that the destroyed corrosion of cell wall of plant cell, between lignin with cell polysaccharide, close physical chemistry is combined and beats
Opening, the parcel of cellulose, hemicellulose is effectively dissociated by lignin, and cellulose is more exposed to material surface, and cellulose is tied
Crystalline substance degree reduces, and specific surface area increases, and intracellular matter is blend-out.
As shown in figure 3-1, corn straw, under the conditions of sodium hydroxide and acidifying steam explosion combined pretreatment, is assisted with composite flora
Same-action, the ability that degrading maize straws produces hydrogen is relatively strong, its produce hydrogen rate and corn straw degradation rate respectively reach 8.6mmol/g and
72%.
As shown in figure 3-2, corn straw is through H2SO4(0.5%w/v) under pretreatment condition, and composite flora synergism,
It produces hydrogen rate and corn straw degradation rate respectively reaches 7.8mmol/g and 65%.
As shown in Fig. 3-3, corn straw is through NH3·H2Under O (5.0%w/v) pretreatment condition, work collaborative with composite flora
With, it produces hydrogen rate and corn straw degradation rate respectively reaches 3.8mmol/g and 33%.
As shown in Figure 3-4, corn straw under NaOH (1.0%w/v) pretreatment condition, and composite flora synergism,
It produces hydrogen rate and corn straw degradation rate respectively reaches 2.8mmol/g and 26%.
By Fig. 3-1 to shown in Fig. 3-4, contrasting four kinds of preprocess methods, result shows, through sodium hydroxide and acidifying steam explosion connection
Closing pretreatment is optimal maize straw pretreatment method.And the not knot of cellulose in the corn straw of combined pretreatment
Crystalline substance degree is high, and between lignin with cell polysaccharide, close physical chemistry is combined, and limits composite flora cellulose degrading enzyme with thin
The contact of cell wall polysaccharide, causes the degraded of corn straw and fermentation and hydrogen production process relatively slower, and produces hydrogen rate and degradation rate all
The lowest, find out from result, without the product hydrogen rate of corn straw of pretreatment and degradation rate only have respectively 1.1mmol/g and
8.8%.
As shown in Fig. 4-1, no matter using which kind of preprocess method, in fermentation and hydrogen production system, reducing sugar does not the most accumulate
Process, is all to reduce rapidly along with the passage of fermentation and hydrogen production time from original pretreated reducing sugar level.After 12h
System is substantially not detectable reducing sugar；This shows the reduction produced in fermentation and hydrogen production system along with degrading maize straws
Sugar, this partial reduction sugar is at once by composite flora strain X9And B2Utilization carries out thalli growth anabolism and production capacity metabolism, thus
Reflect whole during the accumulation of reducing sugar does not the most occur.
As shown in the Fig. 4-2, through sodium hydroxide and acidifying steam explosion combined pretreatment and H2SO4(0.5%w/v) pretreatment condition
Under, during 40h, cell concentration respectively reaches 0.61g/L and 0.53g/L, the most progressively tends towards stability；Through NaOH (1.0%w/v) and
NH3·H2Under O (5.0%w/v) pretreatment condition, its cell concentration when 40h respectively reaches 0.33g/L and 0.34g/L, afterwards
Progressively tend towards stability.
Table 1 is in four kinds of pretreatment corn stalk fiber element efficient anaerobic fermentation bio-hydrogen reaction systems, main liquid phase
The composition of metabolism end products and pH value.
Table 1
As shown in table 1, in four kinds of pretreatment corn stalk fiber element efficient anaerobic fermentation bio-hydrogen reaction systems, mainly
The composition of liquid phase metabolism end products and pH value.In general, along with growth and the raising meeting of metabolic activity of bacterial biomass
Promote the antibacterial degraded utilization rate to substrate, so that the acidification rate of the terminal aqueous production in fermentation liquid system can be corresponding
Improving, pH value can reduce accordingly.There is certain positive correlation between the two.
Understanding in conjunction with Fig. 3-1 to Fig. 3-4, Fig. 4-1 to Fig. 4-2 and table 1, composite flora is utilizing sodium hydroxide and acidifying vapour
During the corn stalk fiber element efficient anaerobic fermentation bio-hydrogen of quick-fried combined pretreatment, VFAs total amount is 2652mg/L, pH
It is 4.3.These indexs are all substantially better than other three kinds of preprocess methods, and this also illustrates sodium hydroxide and the pre-place of acidifying steam explosion associating
Reason is optimal preprocess method, is conducive to the anaerobic fermentation of biological improving composite flora Synergistic degradation corn stalk fiber element to produce
The ability of hydrogen.
Composite flora Synergistic degradation is the most preprocessed through the ability of the corn stalk fiber element fermentation and hydrogen production of pretreatment
Shi Yaogao, preprocess method all has a certain degree of impact to the modification of corn straw process and the ability of raising fermentation and hydrogen production,
So it is all the most necessary, wherein for carrying out corn straw pretreatment to raising composite flora cellulose degradation rate and hydrogen production potential
Composite flora utilizes sodium hydroxide the highest with the hydrogen production potential of acidifying steam explosion combined pretreatment corn straw, H2SO4Pretreatment time
It, NaOH and NH3·H2O pretreatment is relatively low.
The screening of embodiment 3 compound strain
Used strain is taken from laboratory and is flowed stirring slot type hydrogen production through anaerobic fermentation reactor CSTR continuously
(ZL92114474.1) activated sludge in.Through separation, purification obtain hydrogen-producing bacteria 125 strain, to hydrogen production potential stronger seven
Hydrogen production potential and the fermentation end products of strain hydrogen-producing bacteria B2, B19, H7, X9, X12, C3 and C17 carry out gas chromatographic analysis.Root
According to observation by light microscope and end products gas chromatographic analysis, the hydrogen-producing bacteria being separated to being divided into two classes, the first kind is second
Alcohol type fermentation and hydrogen production bacterial strain B2, B19 and H7 etc.；Equations of The Second Kind is butanoic acid type fermentative hydrogen-producing bacteria X9, X12, C3 and C17 etc..To with
Upper seven kinds of hydrogen-producing bacterias carry out the screening of composite flora.
1) hydrogen-producing bacteria separation and screening (primary dcreening operation)
Method is with embodiment 1, simply by step 3) in composite flora replace with single bacterial strain as shown in table 2, connect
The amount of kind is 6%, result such as table 2.Table 2 is bacterial fermentation end primary product composition and hydrogen output.
Table 2
As shown in table 2, hydrogen-producing bacteria B2, B19 show similar fermentation end products, predominantly ethanol and second with H7
Acid, the content of its ethanol and acetic acid accounts for more than the 90% of total end products content, and the content of a high proportion of ethanol and acetic acid embodies
Fermentation and hydrogen production strain significant ethanol-type fermentation feature.The hydrogen output of three strain bacterium the most all reaches 5000～6000ml/L simultaneously,
Show good fermentation and hydrogen production ability.Can be all typical ethanol-type fermentation hydrogen-producing bacteria with preliminary judgement B2, B19 and H7；
Hydrogen-producing bacteria X9, X12, C3 have a similar fermentation end products, predominantly butanoic acid and acetic acid with C17 tetra-strain bacterium, its butanoic acid and
The content of acetic acid accounts for more than the 90% of total end products content, and the hydrogen output of three strain bacterium reaches 2000～3500ml/L, and end is sent out
Butanoic acid and acetic acid content explanation X9, X12 and H17 that in ferment product, content is higher are typical butanoic acid type fermentative hydrogen-producing bacterias.
2) separation of degraded cellulose hydrogen-producing bacteria and screening (multiple sieve)
With microcrystalline Cellulose (MCC) and sodium carboxymethyl cellulose (CMC) for sieving culture medium again, through separating, screening and pure
Change to have successfully been isolated and obtain degraded cellulose and hydrogen-producing bacteria purebred X9, X12 and C17 of fermentation and hydrogen production while that three strains having.
Result such as table 3, table 3 is bacterial fermentation end primary product composition and hydrogen output.
Table 3
As shown in table 3, three strain degraded cellulose hydrogen-producing bacteria X9, X12 and the C17 obtained screening utilize MCC fermentation to produce
The ability of hydrogen and fermentation end products gas chromatographic analysis are it can be seen that three strain antibacterials utilize the hydrogen output of ferment cellulose for producing hydrogen
1000～1700ml/L can be reached, there is higher degraded cellulose fermentation and hydrogen production ability.Simultaneous hydrogen production antibacterial X9, X12 and
The fermentation end products of C17 tri-strain bacterium is all mainly butanoic acid and acetic acid, and its total end products content is 3500～5000mg/L.Its
In there is higher cellulose degradation hydrogen production potential due to strain X 9, show good growth and hydrogen-producing characteristic, therefore select
Strain X 9.
3) composite flora is compounding optimizes
Use the relatively easy and single microcrystalline Cellulose (i.e. MCC, 12g/L) of structure and sodium carboxymethyl cellulose is (i.e.
CMC, 5g/L) as fermentation and hydrogen production substrate, pure for several classes bacterial strain is mutually mixed and compounds, answering of selection traits and metabolic stability
Close flora and be further continued for successive transfer culture.Incubation declines such as degraded cellulose hydrogen production potential, then eliminates or continue compounding.Will be through
More than half a year continuous successive transfer culture, selects the composite flora still keeping higher degraded cellulose hydrogen production potential as test further
For examination strain (seed).Result such as table 4, table 4 is several floras and their product hydrogen rate and degradation rate added.By table 4
Visible with the composite flora that strain X 9 and B2 is combination, its product hydrogen rate and degradation rate are optimal.
Table 4
4) optimization experiment
This test is fermentation and hydrogen production substrate with MCC (12g/L, 100ml), to produce hydrogen rate as measurement index, investigates compound bacteria
Group's different inoculum concentration of X9+B2 and the inoculative proportion impact (being shown in Table 5) on its growing state and the ability of fermentation and hydrogen production.Thus really
Determine the optimal inoculum concentration of composite flora and inoculative proportion.Table 5 is compounded combination optimization Test.
Table 5
When the inoculum concentration of composite flora is 8ml (8%) and inoculative proportion is 1:1 when, produce hydrogen rate and reach 9.8mmol/
g；When the inoculum concentration of composite flora be 6ml (6%) and inoculative proportion also for 1:1 when, its produce hydrogen rate can reach 9.6mmol/
G, at this moment inoculative proportion is not changed in, but inoculum concentration decreases 25%, but product hydrogen rate now reduced by only 2%；Work as inoculation
When amount is less than 6%, slowly, incubation time is long for strain growth propagation, produces enzyme activity and hydrogen production potential is the highest；And inoculum concentration exceedes
When 6%, then strengthen inoculum concentration on enzyme live substantially do not have anything to affect, the most do not significantly improve hydrogen production potential.So being not to connect
Kind amount the biggest, enzymatic productivity is the strongest, produce hydrogen rate the highest, it is impossible to simply by increase inoculum concentration improve enzyme live, Biomass and
Produce hydrogen rate.Furthermore, it is contemplated that the problems such as industrial applications cost, when the inoculum concentration of strain X 9 and B2 is 6ml (6%) and inoculation ratio
When example is also 1:1, reach ideal product hydrogen effect.
It addition, in different compound schemes, along with in composite flora, its hydrogen production potential of increase of strain X 9 inoculum concentration carries
Height is more notable, illustrates that strain X 9 is " leading bacterial strain "；And bacterial strain B2 is along with its product hydrogen of the increase of inoculum concentration in composite flora
The raising of ability is not it is obvious that explanation bacterial strain B2 is " supplementary strain ".Meanwhile, composite flora mainly utilizes cellulase hydrolysis sugar
Change and utilize two effective synergism of biological metabolism of enzymatic saccharification liquid fermentation and hydrogen production to reach efficient degradation ferment cellulose for producing
The purpose of hydrogen, to the extent that, the strain X 9 in composite flora is again relative " of equal importance " with B2.
By analyzing above, the inoculum concentration of composite flora be 6ml (6%) and inoculative proportion be 1:1 be appropriate compounding group
Conjunction mode, now composite flora can obtain higher hydrogen production potential.
Claims (10)
1. a composite flora Synergistic degradation stalk cellulose and the combined pretreatment method of fermentation and hydrogen production, it is characterised in that include
Following steps:
1) straw combined pretreatment: powder of straw is broken into powder of straw, first through NaOH solution pretreatment, more acidified steam explosion is located in advance
Reason, obtains combined pretreatment powder of straw；
2) anaerobic fermentation of biological produces hydrogen: batch fermentation produces hydrogen assay device deoxygenation sterilizing, then by molten for combined pretreatment powder of straw
Yu Shuizhong, obtains stalk cellulose substrate, is placed on agitator, and composite flora is inoculated with the inoculum concentration of 5-10%, seals container,
At 37～40 DEG C, under the conditions of impact strength is 90～130rpm, carrying out cultivating and fermentation and hydrogen production, ferment 48h, collects the gas produced
Body.
Method the most according to claim 1, it is characterised in that: described powder of straw particle diameter is 30～60 mesh.
Method the most according to claim 1, it is characterised in that: described through NaOH solution pretreatment is: by solid-to-liquid ratio 1:
10-15, takes powder of straw and NaOH solution, after soaking at room temperature 20-25h, is washed to neutrality；The percent mass of described NaOH solution
Concentration is 0.5%-2.0%.
Method the most according to claim 3, it is characterised in that: solid-to-liquid ratio is 1:10, the mass percentage concentration of NaOH solution
It is 1.0%.
Method the most according to claim 1, it is characterised in that: described acidified steam explosion pretreatment is: by solid-to-liquid ratio 1:
10-15, the pretreated powder of straw of NaOH solution of learning from else's experience and aqueous sulfuric acid are placed in container, mix, and described sulphuric acid is water-soluble
The concentration expressed in percentage by volume of liquid is 1-1.5%；Container is sealed, carries out steam explosion in being placed in pressure cooker, after steam explosion completes, drop
Temperature, blood pressure lowering, after being cooled to room temperature, rinse to neutral with water, dry, obtain combined pretreatment powder of straw.
Method the most according to claim 5, it is characterised in that: solid-to-liquid ratio is 1:10, and the volume basis of aqueous sulfuric acid is dense
Degree is 1%.
Method the most according to claim 5, it is characterised in that: steam explosion condition is: is sealed by container, is placed in pressure cooker,
In 120-125 DEG C, under pressure 0.15-0.20MPa, steam explosion 2-3h；After steam explosion completes, when pressure cooker temperature is down to 90-95 DEG C, fall
Pressure, is cooled to room temperature, is washed to neutrality, dries, obtain combined pretreatment powder of straw at 80 DEG C.
Method the most according to claim 1, it is characterised in that: described step 2) in, by molten for combined pretreatment powder of straw
Yu Shuizhong, obtains the stalk cellulose substrate that concentration is 10-20g/L, inoculates composite flora by inoculum concentration 6%.
Method the most according to claim 1, it is characterised in that: described composite flora is that strain X 9 and bacterial strain B2 are by 1:1
Mixing.
Method the most according to claim 9, it is characterised in that: the NCBI number of registration of described strain X 9 is EU434651；
The NCBI number of registration of described bacterial strain B2 is EU639425.
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| CN106942473A (en) * | 2017-03-09 | 2017-07-14 | 寿县康瑄山羊养殖有限公司 | It is a kind of to improve the processing method of maize straw digestibility |
| CN107012195A (en) * | 2017-06-12 | 2017-08-04 | 北京中农富源生物工程技术有限公司 | A kind of method using agricultural crop straw as fermenting raw materials hydrogen manufacturing |
| CN108781593A (en) * | 2018-06-20 | 2018-11-13 | 中国水稻研究所 | Prevent the method for the dysfunction factor of damping-off during a kind of rice soilless breeding seeding |
| CN110484571A (en) * | 2019-09-29 | 2019-11-22 | 哈尔滨工业大学 | Utilize the method for corn stover semi-successive cultivation hydrogen and grease |
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| CN106942473A (en) * | 2017-03-09 | 2017-07-14 | 寿县康瑄山羊养殖有限公司 | It is a kind of to improve the processing method of maize straw digestibility |
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| CN107012195B (en) * | 2017-06-12 | 2022-07-12 | 北京中农富源生物工程技术有限公司 | Method for producing hydrogen by fermenting crop straws as raw materials |
| CN108781593A (en) * | 2018-06-20 | 2018-11-13 | 中国水稻研究所 | Prevent the method for the dysfunction factor of damping-off during a kind of rice soilless breeding seeding |
| CN108781593B (en) * | 2018-06-20 | 2022-10-04 | 中国水稻研究所 | Method for preventing damping-off functional disturbance factors in rice soilless seedling raising process |
| CN110484571A (en) * | 2019-09-29 | 2019-11-22 | 哈尔滨工业大学 | Utilize the method for corn stover semi-successive cultivation hydrogen and grease |
| CN110484571B (en) * | 2019-09-29 | 2023-04-25 | 哈尔滨工业大学 | Method for semi-continuously producing hydrogen and grease by using corn straw |
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