CN1296063C - Compound red sage prepn for treating cardiac and cerebral vascular diseases and its prepn process - Google Patents
Compound red sage prepn for treating cardiac and cerebral vascular diseases and its prepn process Download PDFInfo
- Publication number
- CN1296063C CN1296063C CNB021573778A CN02157377A CN1296063C CN 1296063 C CN1296063 C CN 1296063C CN B021573778 A CNB021573778 A CN B021573778A CN 02157377 A CN02157377 A CN 02157377A CN 1296063 C CN1296063 C CN 1296063C
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- CN
- China
- Prior art keywords
- extract
- salviae miltiorrhizae
- radix salviae
- water
- red sage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- DOUMFZQKYFQNTF-MRXNPFEDSA-N rosemarinic acid Natural products C([C@H](C(=O)O)OC(=O)C=CC=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 DOUMFZQKYFQNTF-MRXNPFEDSA-N 0.000 description 1
- TVHVQJFBWRLYOD-UHFFFAOYSA-N rosmarinic acid Natural products OC(=O)C(Cc1ccc(O)c(O)c1)OC(=Cc2ccc(O)c(O)c2)C=O TVHVQJFBWRLYOD-UHFFFAOYSA-N 0.000 description 1
- XHALVRQBZGZHFE-UHFFFAOYSA-N rosmarinic acid methyl ester Natural products C=1C=C(O)C(O)=CC=1C=CC(=O)OC(C(=O)OC)CC1=CC=C(O)C(O)=C1 XHALVRQBZGZHFE-UHFFFAOYSA-N 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention discloses a compound red sage root preparation which is characterized in that the preparation is compounded from 4 to 60 parts by weight of extract of red sage root, 2 to 18 parts by weight of notoginseng extract and 1 to 10 parts by weight of borneol. The disclosed compound red sage root preparation of the present invention has the advantages of advanced technology and controllable quality, and has the functions of resisting myocardial ischemia and platelet agglutination, and treating coronary heart disease, angina pectoris, myocardial infarction, cerebral thrombosis, etc.
Description
Invention field
The present invention relates to a kind of pharmaceutical preparation and preparation method thereof, particularly relate to pharmaceutical preparation for the treatment of cardiovascular and cerebrovascular disease and preparation method thereof.
Background of invention
Cardiovascular and cerebrovascular disease is to cause human first dead sick the kind, and along with the raising of social life level and the aging of population, the research of cardiovascular and cerebrovascular diseases medicament becomes the focus of drug development.Coronary atherosclerotic heart disease, be called for short coronary heart disease, its cause of disease is because some can not infilter blood-lipoids material such as cholesterol, the triglyceride etc. of arterial wall, be deposited on and form " atheromatous plaque " back on the endarterium because of calmness calcareous in the liquid, make original whippy tremulous pulse hardening, along with the atheromatous plaque scope on the endarterium strengthens, cause lumen of vessels to narrow down even block, cause myocardial ischemia, anoxia or necrosis.Ischemia apoplexy (cerebral infarction) all is important opportunitys of its prevention and treatment at acute attack stage and in apoplexy early stage and convalescent period.Chinese medicine plays an important role in above-mentioned prevention and treatment of diseases, especially is that the folk prescription or the compound preparation of primary raw material all has definite curative effect with the Radix Salviae Miltiorrhizae, as FUFANG DANSHEN PIAN, FUFANG DANSHEN ZHUSHEYE, treating coronary heart disease granule, happy amylan capsule etc.Radix Salviae Miltiorrhizae is being brought into play main effect in these preparations.
Radix Salviae Miltiorrhizae is the root of Labiatae salvia Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bge..Bitter in the mouth, cold nature has blood circulation promoting and blood stasis dispelling, nourishing blood to tranquillize the mind, the effect of removing heat from blood row's carbuncle and toxin expelling granulation promoting is the common drug of Chinese medicine blood circulation promoting and blood stasis dispelling.The red rooted salvia master contains fat-soluble Diterpenes composition and water miscible liposoluble ingredient, still contains other compositions such as flavonoid, triterpenes, sterol.What belong to quinone, ketone type structure in the Diterpenes composition has Tanshinone I, an II
A, II
B, V, VI, cryptotanshinone, iso tanshinone I, II, II
B, dihydrotanshinone I etc.Water miscible liposoluble ingredient has depside that danshensu, protocatechualdehyde, protocatechuic acid, caffeic acid and danshensu and caffeinic derivant or dimer esterification form such as salvianolic acid A, B, C, D, E, G, alkannic acid B, rosmarinic acid, rosmarinic acid methyl ester etc.Tanshinone I I
AIt is one of representative composition of Radix Salviae Miltiorrhizae Diterpenes blood circulation promoting and blood stasis dispelling.Modern pharmacological research shows Tanshinone I I
AMonomeric pharmacological action mainly contains: expeirmental myocardial ischemia and reperfusion injury are had the improvement effect; Influence experiment to cardiac electrophysiology shows calcium antagonism; The antiplatelet aggregative activity anti thrombotic action.On behalf of the composition cryptotanshinone, the Radix Salviae Miltiorrhizae Diterpenes another have the antibacterial and estrogen-like effects of antiinflammatory.The pharmacological action of water-soluble phenolic acids monomeric compound mainly contains in the Radix Salviae Miltiorrhizae: anti thrombotic action comprises antiplatelet effects, anticoagulation, anti-thrombosis function; Improve the blood circulation effect; The anti-oxidative damage effect.The limitation of existing red sage formulation technology shows as: 1, the unstability of tanshinone, concentrate, in the dry run loss more, cause the low and content poor reproducibility of tanshinone retention rate in preparation; Tanshinone poorly soluble, dissolution is low in the preparation.Existing preparation does not screen suitable adjuvant and technology, to improve the tanshinone dissolution; 2, the extraction of water solublity salvianolic acid class is index with the danshensu, and loss of active ingredients such as danshensu is bigger in the precipitate with ethanol process for refining, and retention rate is low in preparation; Absorb in the danshensu body, metabolism is fast, ordinary preparation is difficult to keep the stability of oral administration blood drug level.Therefore when the exploitation red sage formulation, not only should cure mainly, extract its effective site of reservation according to the prescription function; And should in moulding process, impose the preparation factor, improve the release of effective ingredient and making with extra care of preparation.
Summary of the invention
The object of the invention is to provide a kind of compound red sage root preparation; The object of the invention also is to provide a kind of preparation method of compound red sage root preparation; The object of the invention also is to provide a kind of method of quality control of compound red sage root preparation.
The present invention seeks to be achieved through the following technical solutions:
Preparation of the present invention is made by following raw material:
Radix Salviae Miltiorrhizae extract 4-60 weight portion, Radix Notoginseng extract 2-18 weight portion, Borneolum Syntheticum 1-10 weight portion.
The above-mentioned raw materials optimum ratio is: Radix Salviae Miltiorrhizae extract 36-54 weight portion, Radix Notoginseng extract 8.46-16.92 weight portion, Borneolum Syntheticum 2-8 weight portion; The above-mentioned raw materials optimum ratio is: Radix Salviae Miltiorrhizae extract 18-27 weight portion, Radix Notoginseng extract 4.23-8.46 weight portion, Borneolum Syntheticum 1-2 weight portion; The above-mentioned raw materials optimum ratio is: Radix Salviae Miltiorrhizae extract 4.5-9 weight portion, Radix Notoginseng extract 2.82-5.64 weight portion, Borneolum Syntheticum 4-6 weight portion.
Radix Salviae Miltiorrhizae extract of the present invention can be that red sage root super critical extract, tanshinol extract, Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract, Radix Salviae Miltiorrhizae water extract or Radix Salviae Miltiorrhizae water were carried the refining thing of post; Radix Salviae Miltiorrhizae extract of the present invention can also be that Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water were carried refining thing of post and red sage root super critical extract or tanshinol extract in 1-99: the compositions of 99-1 ratio; Described Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water were carried refining thing of post and red sage root super critical extract or tanshinol extractive composition percentage: 80-20: 20-80,70-30: 30-70,60-40: 40-60 or 50: 50.
Radix Notoginseng extract of the present invention is Radix Notoginseng water extract or Radix Notoginseng ethanol extract; Borneolum Syntheticum of the present invention can replace with Camphora.
Red sage root super critical extract of the present invention or tanshinol extract contain tanshinone and are no less than 5-10%; Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water were carried the refining thing of post and are contained danshensu and be no less than 0.5-1.5%, and protocatechualdehyde is no less than 0.16-0.48%; Radix Notoginseng water extract or Radix Notoginseng ethanol extract contain the ginsenoside Rg
1Be no less than 15.83-30%, contain the ginsenoside Rg
1And Rb
1Be no less than 31-40%.
Preparation of the present invention can add adjuvant and make clinical acceptable forms such as tablet, capsule, oral liquid, drop pill, spray, granule; Described adjuvant comprises solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, substrate etc.
Preparation of the present invention is as follows
A, FUFANG DANSHEN DIWAN
Press recipe quantity, with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum are pulverized, and mix homogeneously is stand-by; Press medicated powder: substrate 1: 1---2 with after substrate PEG6000 or the PEG4000 fusion, and the adding medicament mixed is even, and 60---90 ℃ of insulations, with 20---70 droplets/minute add in the methyl-silicone oils, collect drop pill, absorb liquid coolant with filter paper, the heavy 20-80mg of every ball.
B, FUFANG DANSHEN JIAONANG:
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously add adjuvant 1-3 doubly, make capsule.
C, compound Salviae Miltiorrhizae granule
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously add the adjuvant that suitable medicine 2-5 doubly measures, and make soft material with the ethanol of 60-90% concentration, make granule.
D, compound red sage root spray
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by, add 10～20% tween 80 alcoholic solution, 30～300 parts by volume, add 1,2-propylene glycol 100～500 parts by volume, mixing, ethanol is fixed molten to 200～1000 parts by volume g/ml, be sub-packed in the aerosol pressure bottle by 20-100ml (specification), in every bottle, be pressed into dichlorodifluoromethane 1～10 weight portion behind the gland, make spray.
E, compound Salviae Miltiorrhizae oral liquid
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum mix homogeneously add 1-3 and doubly measure (g/ml) tween 80, add the injection water again and make oral liquid.
The preparation of Radix Salviae Miltiorrhizae extract of the present invention can be but be not limited in the following method any one:
1, gets red rooted salvia, supercritical CO
2Extraction adds 10-40% medical material amount 75-95% ethanol and makes entrainer, extracting pressure 20-40Mpa, temperature 30-60 ℃, gets the red sage root super critical extract.
2, get red rooted salvia, behind the supercritical extraction, medicated powder is taken out, decocting boils 1-2 time, add 6-8 times of water gaging respectively, decocted 0.5-1 hour, merge decocting liquid, concentrate, add 75-95% ethanol and make and contain alcohol amount and reach 60-80%, left standstill 12-24 hour, filter, the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract.
3, get red rooted salvia, behind the supercritical extraction, medicated powder is taken out, decocting boils 1-2 time, adds 4-8 times of water gaging respectively, decocts 1-2 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, the 30-80% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing of post.
4, get red rooted salvia, decocting boils 2-3 time, adds 4-8 times of water gaging respectively, decocted 1-2 hour, and merged decocting liquid, being concentrated into relative density is 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, the 30-80% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing of post.
5, red rooted salvia, 95% alcohol reflux 1-3 time, each 1-2 hour, reclaim ethanol, concentrate drying gets the tanshinol extract.
The preparation of Radix Notoginseng extract of the present invention can be but be not limited in the following method any one:
1, get pseudo-ginseng, pulverize, decocting boils 2---and 3 times, add water 4 respectively---6 times, decocted 1-2 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, the 30-80% ethanol elution reclaims ethanol, concentrate drying gets the Radix Notoginseng water extract.
2, get pseudo-ginseng, pulverize, add 60-70% alcohol reflux 2-3 time, each 1-2 hour, extracting solution reclaimed ethanol, and being concentrated into relative density is 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, the 30-80% ethanol elution, reclaim ethanol, concentrate drying gets the Radix Notoginseng ethanol extract.
Preparation of the present invention is except that being raw material directly feeds intake from extract, can also be the raw material preparation that directly feeds intake by raw medicinal material:
Get red rooted salvia 130-450 weight portion and pseudo-ginseng 25-145 weight portion, be ground into coarse grain, decocting boils 2-3 time, adds water 4-6 respectively and doubly measures, and decocts 1-2 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, the 30-80% ethanol elution reclaims ethanol, concentrate drying, Salvia miltiorrhiza and Panax notoginseng is closed and is carried thing, add Borneolum Syntheticum fine powder 1-8 weight portion, add adjuvant and make tablet, capsule, oral liquid, drop pill, spray, granule, described adjuvant comprises solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, substrate etc.
The raw medicinal material proportioning can also be to get red rooted salvia 130-240 weight portion, pseudo-ginseng 28-60 weight portion, Borneolum Syntheticum or Camphora 1.5-5 weight portion.
The described macroporous resin column model of this description can be: D101, DA201, DM130, WLD-3, YPR-II, X5, AB-8, HPD-100, Dx-5, NKA-9.
Contain in the following assay one or more in the method for quality control of the present invention:
1, danshensu, protocatechualdehyde (according to high performance liquid chromatography)
It is an amount of to get each preparation, puts in the tool plug triangular flask, adds methanol 25ml, supersound process 2 hours, put coldly, add methanol and supply weight, shake up, filter, get filtrate 10ml, water-bath is concentrated into dried, adds water 10ml, ether 20ml is transferred in the separatory funnel, divide and get ether layer, water layer is used 10ml extracted with diethyl ether 2 times more respectively, merge ether solution, ether solution is washed with 5ml, washing liquid is incorporated above-mentioned water layer into, volatilizes ether, steams to a small amount of, add methanol constant volume to the 10ml volumetric flask, being need testing solution, is contrast with the methanol solution of danshensu sodium, and the chromatographic column octadecylsilane chemically bonded silica is a filler, 2-5: 4-1: 95-99 methanol-heptanesulfonic acid sodium solution-water or 75-85: 22-16: 0.5-2 water-methanol-glacial acetic acid is a mobile phase, and the detection wavelength is 280nm.Each preparation is a unit quantity to take output suitable every day, and the per unit amount contains danshensu and is no less than 2.0-4.40mg, and protocatechualdehyde is no less than 0.13-1.44mg;
2, tanshinone, cryptotanshinone (according to high performance liquid chromatography)
Get 1 ether solution, evaporate to dryness, methanol constant volume is contrast to 10ml with the methanol solution of TANSHINONES and the methanol solution of cryptotanshinone, is filler with octadecylsilane chemically bonded silica, and 70-80: the 30-20 methanol-water is a mobile phase, and the detection wavelength is 270nm.Each preparation is a unit quantity to take output suitable every day, and the per unit amount contains tanshinone and is no less than 3.5-7.2mg, and cryptotanshinone is no less than 3.5mg-7.2mg.
3, Borneolum Syntheticum (according to gas chromatography)
It is an amount of to get Pentadecane, and it is fixed molten to add ethyl acetate, as inner mark solution; It is an amount of to get each preparation finished product, adds inner mark solution, and supersound process 2 hours filters, and gets filtrate, is contrast with the inner mark solution of Borneolum Syntheticum, is immobile phase with Polyethylene Glycol-20M, and coating concentration is 10%, and column temperature is 140 ℃.Each preparation is a unit quantity to take output suitable every day, and the per unit amount contains Borneolum Syntheticum and is no less than 20-50mg.
4, ginsenoside Rb
1And ginsenoside Rg
1(according to high performance liquid chromatography)
Get 1 following test sample, with ginsenoside Rb
1And ginsenoside Rg
1Methanol solution be contrast, second cyanogen-with octadecylsilane chemically bonded silica is filler, 55-65: 45-35 methanol-water or 90-110: 410-390,0.05% phosphoric acid solution or 1-4: 9-6 second cyanogen-water is mobile phase, the detection wavelength is 203nm; Perhaps use amino normal phase column, with 85-90: 15-10 second cyanogen-1% phosphoric acid is mobile phase, and the detection wavelength is 203nm.Each preparation is a unit quantity to take output suitable every day, and the per unit amount contains the ginsenoside Rg
1Be no less than 6.67-21.43mg, contain the ginsenoside Rg
1And Rb
1Total amount is no less than 13.3-42.86mg.
Per unit amount content can also be in the above-mentioned method of quality control: tanshinone is no less than 3.53-7.06mg, and cryptotanshinone is no less than 3.53-7.06mg, and danshensu is no less than 2.1-4.2mg, and protocatechualdehyde is no less than 0.13-0.26mg, contains the ginsenoside Rg
1Be no less than 6.67-13.3mg, contain the ginsenoside Rg
1And Rb
1Total amount is no less than 13.3-26.6mg.
Described each preparation finished product amount of regularlying sample and deciding in right amount of getting.
Compound red sage root preparation of the present invention, compare technology advanced person, few, the determined curative effect of dose with official preparation, Pharmacodynamic test of active extract result shows: have and resist myocardial ischemia and effects such as anti-platelet aggregation, treatment coronary heart disease, angina pectoris, myocardial infarction, cerebral thrombosis.These pharmacological actions are confirmed by following pharmacodynamic experiment example.Following experimental example is used to further specify the present invention.
The anti thrombotic action of experimental example 1 compound Salviae Miltiorrhizae tablet of the present invention
Get 40 of rats, be divided into 4 groups at random, press 6 of human dosage, 12 times of amounts are established the dosage group, every day gastric infusion once, successive administration 7 days, after the administration in the 4th day behind the 30min, except that the blank group, all the other animal subcutaneous injection adrenalin hydrochloride injection Adr (0.1%) 0.1ml/, 30min is placed on the 5min that swims in 1.5 degrees centigrade of water, repeated swimming on the 5th day under the same conditions once, 30min after the administration in the 6th day injects Adr one time, 30min after the last administration again, in animal lumbar injection pentobarbital sodium 35mg/kg, anesthetized rat is got blood from rat aorta then, surveys thrombosis.The result shows: compound red sage root preparation can obviously suppress the formation of blood stasis rats in vitro thrombosis, reduces the weight of thrombosis, shortens the length of thrombosis.See Table 1.
The influence that table 1 compound red sage root preparation forms stasis syndrome rat model external thrombus
| Group | Thrombosis long (cm) | Weight in wet base (mg) | Dry weight (mg) |
| The high red sage formulation of blank group model matched group red sage formulation is low | 2.56±0.90 *** 9.11±2.40 5.79±1.75 ** 6.70±2.42 | 143.3±4.22 *** 484.1±144.7 265.3±71.7 *** 353.0±121.1 * | 37.5±13.05 *** 130.0±39.46 67.6±20.75 ** 98.2±38.99 |
Compare with model control group
*P＜0.05,
*P＜0.01,
* *P＜0.001
The influence that experimental example 2 FUFANG DANSHEN DIWAN of the present invention are learned stasis syndrome model mouse hemorheology
Grouping, administration, modeling and processing are with experimental example 1.Get blood from rat aorta and survey its whole blood contrast viscosity, plasma viscosity, packed cell volume and erythrocyte sedimentation rate, the results are shown in Table 2.
Table 2 red sage formulation is to the hemorheology influence of stasis syndrome rat model
| Group | Whole blood contrast viscosity | Plasma viscosity | Packed cell volume (%) | Erythrocyte sedimentation rate (nm/n) | |
| Height is cut | Low cutting | ||||
| The high red sage formulation of blank group model matched group red sage formulation is low | 6.194±1181 * 6.499±1.977 * 6.109±1.004 * 6.189±.002 * | 9.120±1924 ** 10.064±1.765 9.268±3451 * 10.521±4.413 | 1.679±0.071 1.602±0.152 1.615±0.069 1.617±0154 | 41.0±3.399 43.4±1.647 40.9±3.855 39.1±3929 ** | 1.40±0.966 1.72±0.755 2.06±1.208 1.70±0.789 |
Compare with model control group
*P＜0.05,
*P＜0.01,
As seen from Table 2, compound red sage root preparation can reduce the whole blood contrast viscosity of stasis syndrome rat, and other index is not had obvious influence.
Experimental example 3 compound Salviae Miltiorrhizae granules are to the influence of MDA content in LDH, CPK and the cardiac muscular tissue in the myocardial ischemia mice serum due to the isoproterenol
Get 50 of mices, female, body weight 20 ± 2g, divide equally 5 groups by body weight, in the 7th day, press 5 of human dosage, 10,15 times of amounts are established the dosage group, every day gastric infusion once, the 1st, give consubstantiality hydrops 10ml/kg for 2 groups, after administration 4-6 days, the 1st group of subcutaneous injection normal saline 10ml/kg, 2-8 group subcutaneous injection isoproterenol 4mg/kg, 24h after last is given isoproterenol is after the socket of the eye vein is got blood, measure lactic acid dehydrogenase (LDH) and creatine phosphokinase (CPK) content in the mice serum, the muscular tissue of coring is surveyed malonaldehyde (MDA) content, and the result shows, compound red sage root preparation can obviously reduce because the activity of LDH and CPK in the myocardial ischemia mice serum due to the isoproterenol, and mda content in the cardiac muscular tissue, and there were significant differences; See Table 3.
Table 3 compound red sage root preparation is to the influence of LDH and CPK in the myocardial ischemia mice serum due to the isoproterenol
| Group | LDH (U/dL) | CPK (U/DL) | MDA (nmol/L) |
| Blank group model matched group compound red sage root preparation compound red sage root preparation compound red sage root preparation | 836±105 ** 1015±107 797±54 *** 828±155 ** 890±100 | 7.58±0.963 ** 12.82±4.310 8.27±1.182 ** 9.89±1.470 * 10.53±0.851 | 57.4±12.0 * 70.5±10.56 60.8±14.9 * 57.8±10.9 * 59.8±8.43 * |
Compare with model control group
*P＜0.05,
*P＜0.01,
* *P＜0.001
Experimental example 4 FUFANG DANSHEN JIAONANG are to the influence of myocardial ischemia mice
Get 50 of male mices, divide equally 5 groups, establish the dosage group by 5,10,15 times of amounts of human dosage by body weight, gastric infusion, be administered once every day, after the last administration 30 minutes, subcutaneous injection (except first group) isoproterenol 5ml/kg does anti-weary oxygen experiment after 15 minutes.The result shows, compound red sage root preparation can obviously prolong myocardial ischemia mouse diing time due to the isoproterenol, and significant difference is arranged, and sees Table 4.
Table 4 compound red sage root preparation is to the influence of the weary oxygen of myocardial ischemia mouse anti due to the isoproterenol
| Group | Death time (branch) |
| Blank group model matched group compound red sage root preparation compound red sage root preparation compound red sage root preparation | 31.8±8.63 *** 16.0±3.65 22.2±5.67 ** 20.6±4.12 * 19.3±4.06 |
Compare with model control group
*P＜0.05,
*P＜0.01,
* *P＜0.001
Following embodiment all can realize above-mentioned effect.
Embodiment 1: FUFANG DANSHEN PIAN
Radix Salviae Miltiorrhizae water was carried post thing 36g Radix Notoginseng extract 8.46g Borneolum Syntheticum 2g, made 400 in tablet, and per 2,2 times/day.
Embodiment 2: FUFANG DANSHEN JIAONANG
Tanshinol extract 18g, Radix Notoginseng alcohol extract 4.23g, Borneolum Syntheticum 3g makes 270 of capsules, one time 1,2-3 time/day.
Embodiment 3: the compound Salviae Miltiorrhizae granule
Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 54g, pseudo-ginseng water extract 16.92g, Camphora 8g makes granule 800 grams, and 4g/ time, 2-3 time/day.
Embodiment 4:
Radix Salviae Miltiorrhizae water was carried column extract 27g, red sage root super critical extract 4.5g, Radix Notoginseng alcohol extract 8.46g, Borneolum Syntheticum 2g makes spray 1000ml, every day 10-15ml.
Embodiment 5:
Red sage root super critical extract 4.5g, Radix Salviae Miltiorrhizae water extract 36g, pseudo-ginseng water extract 2.82g, Borneolum Syntheticum 1.5g makes 500 of capsules, each 1-2 grain, 2-3 time/day.
Embodiment 6:
Red sage root super critical extract 8g, Radix Notoginseng alcohol extract 5,64g, Borneolum Syntheticum 3g makes 260 of capsules, and each 1,1-2 time/day.
Embodiment 7:
Red sage root super critical extract 9g, pseudo-ginseng water extract 8.46g, Borneolum Syntheticum 2.54g makes 180 of capsules, and each 1,1-2 time/day.
Embodiment 8:
Red sage root super critical extract 18g, Radix Salviae Miltiorrhizae water was carried column extract 27g, pseudo-ginseng water extract 15g, Borneolum Syntheticum 3g makes 400 of soft capsules, and each 1,1-2 time/day.
Embodiment 9: FUFANG DANSHEN DIWAN
Radix Salviae Miltiorrhizae 133g, Radix Notoginseng 26.3g, Borneolum Syntheticum 1.5g gets red rooted salvia and pseudo-ginseng, be ground into coarse grain, decocting boils three times, adds 6 times in water respectively, 5 times, 5 times, decocted 1,1,1 hour, merge decocting liquid, be concentrated into relative density and be 1.04, centrifugal, supernatant is crossed the D101 macroporous resin column, washing is abandoned water lotion, 60% ethanol elution, reclaim ethanol, concentrate drying, Salvia miltiorrhiza and Panax notoginseng is closed and is carried thing 7.97g; Borneolum Syntheticum 1.5g is ground into fine powder, closes with Salvia miltiorrhiza and Panax notoginseng and carries the thing mix homogeneously; By medicine: substrate adds substrate PEG6000 14.21g at 1: 1.5, and mix homogeneously splashes in the methyl-silicone oil, makes drop pill, totally 960 balls, every ball weight 25mg, consumption per day 30 balls.One time 10 ball, 2-3 time/day.
Embodiment 10: the compound Salviae Miltiorrhizae granule
Radix Salviae Miltiorrhizae 14Og, Radix Notoginseng 28g, Borneolum Syntheticum 2g, get red rooted salvia and pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6 times in water respectively, 5 times, decocted 1,1 hour, merge decocting liquid, being concentrated into relative density is 1.04, leaves standstill, the DA201 macroporous resin column is crossed in ultrafiltration, filtrate, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying, must make with extra care thing 7.97g, Borneolum Syntheticum 2g is ground into fine powder, mix homogeneously, press medicated powder: adjuvant adds dextrin 40g at 1: 4, make granule 50g, a 1g, 2-3 time/day.
Embodiment 11: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 8g gets red rooted salvia and pulverizes (20 order), supercritical CO
2Extraction adds 30% medical material amount, 90% ethanol and makes entrainer, 50 ℃ of extracting pressure 28MPa, temperature, get extract 8g, behind the supercritical extraction, medicated powder is taken out, decocting boils secondary, adds 8,6 times of water gagings respectively, decocts 1,1 hour, merge decocting liquid, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, left standstill 12-24 hour, filter, the filtrate concentrate drying, 54g gets dry extract; Get pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6,4 times in water respectively, decocted 1,1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 7.5g;
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized (80 order) mix homogeneously; Press medicated powder: the adding medicament mixed was even with after the substrate PEG6000 fusion in 1: 1.5 for substrate, and 70 ℃ of insulations in 30 droplets/minute adding methyl-silicone oils, are collected drop pill, absorb liquid coolant with filter paper, make 2800 balls, the heavy 70mg of every ball, and day is obeyed 25 balls.
Drop pill method of quality control assay:
1, danshensu, protocatechualdehyde (according to high performance liquid chromatography)
Get drop pill 25 balls, put in the tool plug triangular flask, add methanol 25ml, supersound process 2 hours, put coldly, add methanol and supply weight, shake up, filter, get filtrate 10ml, water-bath is concentrated into dried, adds water 10ml, ether 20ml is transferred in the separatory funnel, divide and get ether layer, water layer is used 10ml extracted with diethyl ether 2 times more respectively, merge ether solution, ether solution is washed with 5ml, washing liquid is incorporated above-mentioned water layer into, volatilizes ether, steams to a small amount of, add methanol constant volume to the 10ml volumetric flask, being need testing solution, is contrast with the methanol solution of danshensu sodium, and the chromatographic column octadecylsilane chemically bonded silica is a filler, methanol-heptanesulfonic acid sodium solution-water was mobile phase in 3: 2.5: 97, and the detection wavelength is 280nm.
2, tanshinone, cryptotanshinone (according to high performance liquid chromatography)
Get 1 ether solution, evaporate to dryness, methanol constant volume is contrast to 10ml with the methanol solution of TANSHINONES and the methanol solution of cryptotanshinone, is filler with octadecylsilane chemically bonded silica, and 73: 27 methanol-waters are mobile phase, and the detection wavelength is 270nm.
3, Borneolum Syntheticum (according to gas chromatography)
It is an amount of to get Pentadecane, and it is fixed molten to add ethyl acetate, as inner mark solution; Get drop pill 25 balls, add inner mark solution, supersound process 2 hours filters, and gets filtrate, is contrast with the inner mark solution of Borneolum Syntheticum, is immobile phase with Polyethylene Glycol-20M, and coating concentration is 10%, and column temperature is 140 ℃.
4, ginsenoside Rb
1And ginsenoside Rg
1(according to high performance liquid chromatography)
Get 1 following test sample, with ginsenoside Rb
1And ginsenoside Rg
1Methanol solution for the contrast, be filler with octadecylsilane chemically bonded silica, 58: 42 methanol-waters are mobile phase, the detection wavelength is 203nm, the detection wavelength is 203nm.
The contained danshensu of every drop pill of present embodiment is no less than 0.096mg, and protocatechualdehyde is no less than 0.031mg.Every contained tanshinone of drop pill is no less than 0.16mg, and cryptotanshinone is no less than 0.16mg.Every contained Borneolum Syntheticum of drop pill is no less than 1.57mg in Borneolum Syntheticum.Every contained Radix Notoginseng of drop pill is with the ginsenoside Rg
1Meter is no less than 0.42mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 0.84mg.
Embodiment 12 compound Salviae Miltiorrhizae granules
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 8g gets red rooted salvia and pulverizes (20 order), supercritical CO
2Extraction adds 30% medical material amount, 90% ethanol and makes entrainer, 50 ℃ of extracting pressure 28MPa, temperature, get extract 8g, behind the supercritical extraction, medicated powder is taken out, decocting boils secondary, adds 8,6 times of water gagings respectively, decocts 1,1 hour, merge decocting liquid, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, left standstill 12-24 hour, filter, the filtrate concentrate drying, 54g gets dry extract; Get pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6,4 times in water respectively, decocted 1,1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 7.5g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add the dextrin of 3 times of amounts of suitable medicine, make soft material, make granule 300g, each 3g, day clothes 1-2 time with the ethanol of 80% concentration.
Embodiment 13 FUFANG DANSHEN JIAONANG
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 8g gets red rooted salvia and pulverizes (20 order), supercritical CO
2Extraction adds 30% medical material amount, 90% ethanol and makes entrainer, 50 ℃ of extracting pressure 28MPa, temperature, get extract 8g, behind the supercritical extraction, medicated powder is taken out, decocting boils secondary, adds 8,6 times of water gagings respectively, decocts 1,1 hour, merge decocting liquid, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, left standstill 12-24 hour, filter, the filtrate concentrate drying, 54g gets dry extract; Get pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6,4 times in water respectively, decocted 1,1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 7.5g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The dextrin that adds 2 times of amounts of suitable medicine is made 900 of capsules, and each 3, day clothes 3 times.
Embodiment 14 compound Salviae Miltiorrhizae soft capsules
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 8g gets red rooted salvia and pulverizes (20 order), supercritical CO
2Extraction adds 30% medical material amount, 90% ethanol and makes entrainer, 50 ℃ of extracting pressure 28MPa, temperature, get extract 8g, behind the supercritical extraction, medicated powder is taken out, decocting boils secondary, adds 8,6 times of water gagings respectively, decocts 1,1 hour, merge decocting liquid, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, left standstill 12-24 hour, filter, the filtrate concentrate drying, 54g gets dry extract; Get pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6,4 times in water respectively, decocted 1,1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 7.5g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The vegetable oil that adds 1 times of amount of suitable medicine, even with the colloid mill mill, make 500 of soft capsules, each 2, day clothes 2 times.
Embodiment 15 compound red sage root sprays
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 8g gets red rooted salvia and pulverizes (20 order), supercritical CO
2Extraction adds 30% medical material amount, 90% ethanol and makes entrainer, 50 ℃ of extracting pressure 28MPa, temperature, get extract 8g, behind the supercritical extraction, medicated powder is taken out, decocting boils secondary, adds 8,6 times of water gagings respectively, decocts 1,1 hour, merge decocting liquid, concentrate, add 95% ethanol and make and contain the alcohol amount and reach 70%, left standstill 12-24 hour, filter, the filtrate concentrate drying, 54g gets dry extract; Get pseudo-ginseng, be ground into coarse grain, decocting boils 2 times, add 6,4 times in water respectively, decocted 1,1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 7.5g;
Radix Salviae Miltiorrhizae is extracted, thing Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by, and 20% tween 80 alcoholic solution 100ml adds 1,2-propylene glycol 200ml, mixing, ethanol is fixed molten to 1000ml, and ethanol is fixed molten to 1000ml, the 50ml/ bottle is sub-packed in the aerosol pressure bottle, in every bottle, be pressed into dichlorodifluoromethane 2g behind the gland, make spray, daily 10-15ml.
Embodiment 16: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g,
Get red rooted salvia and pulverize (20 order), supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 1 time, adds 6 times of water gagings, decocts 0.5 hour, merges decocting liquid, concentrates, and adds 95% ethanol and makes and contain the alcohol amount and reach 70%, leaves standstill 12 hours, filters, and the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 70% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 4.23g;
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized (80 order) mix homogeneously; Press medicated powder: the adding medicament mixed was even with after the substrate PEG4000 fusion in 1: 1.3 for substrate, and 80 ℃ of insulations in 40 droplets/minute adding methyl-silicone oils, are collected drop pill, absorb liquid coolant with filter paper, and the heavy 60mg of every ball makes 1944 balls, and day is obeyed 18 balls.
Embodiment 17: the compound Salviae Miltiorrhizae granule
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g,
Get red rooted salvia and pulverize (20 order), supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 1 time, adds 6 times of water gagings, decocts 0.5 hour, merges decocting liquid, concentrates, and adds 95% ethanol and makes and contain the alcohol amount and reach 70%, leaves standstill 12 hours, filters, and the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 70% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 4.23g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add the microcrystalline Cellulose of 4 times of amounts of suitable medicine, make soft material, make granule 250g, each 3g, day clothes 1-2 time with the ethanol of 70% concentration.
Press embodiment 10 method of quality control and measure, the mobile phase of the assay of danshensu, protocatechualdehyde is 80: 19: 1 water-methanol-glacial acetic acid, and the contained danshensu of every g is no less than 1.08mg, and protocatechualdehyde is no less than 0.36mg.The contained tanshinone of every g is no less than 1.8mg, and cryptotanshinone is no less than 1.8mg.The contained Borneolum Syntheticum of every g is no less than 13.2mg in Borneolum Syntheticum.The contained Radix Notoginseng of every g is with the ginsenoside Rg
1Meter is no less than 4.28mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 8.56mg.
Embodiment 18: FUFANG DANSHEN JIAONANG
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g,
Get red rooted salvia and pulverize (20 order), supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 1 time, adds 6 times of water gagings, decocts 0.5 hour, merges decocting liquid, concentrates, and adds 95% ethanol and makes and contain the alcohol amount and reach 70%, leaves standstill 12 hours, filters, and the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 70% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 4.23g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The microcrystalline Cellulose that adds 2 times of amounts of suitable medicine is made 600 of capsules, and each 2, day clothes 3 times.
Embodiment 19: compound red sage root spray
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g,
Get red rooted salvia and pulverize (20 order), supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 1 time, adds 6 times of water gagings, decocts 0.5 hour, merges decocting liquid, concentrates, and adds 95% ethanol and makes and contain the alcohol amount and reach 70%, leaves standstill 12 hours, filters, and the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 70% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 4.23g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by, 10% tween 80 alcoholic solution 120ml adds 1,2-propylene glycol 80ml, mixing, ethanol is fixed molten to 1000ml, and the 50ml/ bottle is sub-packed in the aerosol pressure bottle, is pressed into dichlorodifluoromethane 7g behind the gland in every bottle, make spray, daily 10-15ml.
Embodiment 20: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 400g, Radix Notoginseng 120g, Borneolum Syntheticum 6g gets red rooted salvia and pulverizes supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 50 ℃ of extracting pressure 30MPa, temperature get red sage root super critical extract red sage root super critical extract 6.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 2 times, add 4 times of water gagings respectively, decocted 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 75% ethanol elution reclaims ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 18g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 4.23g
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized mix homogeneously; Press medicated powder: the adding medicament mixed was even, 70 ℃ of insulations with after substrate PEG6000 or the PEG400 fusion in 1: 1.8 for substrate, with inside/outside directly is to add in the methyl-silicone oil with 50 droplets/minute, collects drop pill, absorbs liquid coolant with filter paper, make 2431 balls, the heavy 40mg of every ball, day clothes 25 balls.
Embodiment 21: the compound Salviae Miltiorrhizae granule
Get Radix Salviae Miltiorrhizae 400g, Radix Notoginseng 120g, Borneolum Syntheticum 6g gets red rooted salvia and pulverizes supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 50 ℃ of extracting pressure 30MPa, temperature get red sage root super critical extract red sage root super critical extract 6.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 2 times, add 4 times of water gagings respectively, decocted 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 750% ethanol elution reclaims ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 18g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 4.23g
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add the carboxymethyl cellulose of 4 times of amounts of suitable medicine, make soft material, make granule 180g, each 2g, day clothes 1-2 time with the ethanol of 60% concentration.
Embodiment 22: FUFANG DANSHEN JIAONANG
Get Radix Salviae Miltiorrhizae 400g, Radix Notoginseng 120g, Borneolum Syntheticum 6g gets red rooted salvia and pulverizes supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 50 ℃ of extracting pressure 30MPa, temperature get red sage root super critical extract red sage root super critical extract 6.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 2 times, add 4 times of water gagings respectively, decocted 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 750% ethanol elution reclaims ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 18g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 4.23g
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The carboxymethyl cellulose that adds 2 times of amounts of suitable medicine is made 400 of capsules, and each 2, day clothes 4 times.
Press embodiment 10 method of quality control and measure the ginsenoside Rg
1With ginsenoside Rb
1The mobile phase of assay be 99: 400 second cyanogen-0.05% phosphoric acid solutions, the contained danshensu of every capsules is no less than 0.48mg, protocatechualdehyde is no less than 0.16mg, the contained tanshinone of every capsules is no less than 1mg, cryptotanshinone is no less than 1mg, the contained Borneolum Syntheticum of every capsules is no less than 8.25mg in Borneolum Syntheticum, and the contained Radix Notoginseng of every capsules is with the ginsenoside Rg
1Meter is no less than 2.28mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 4.56mg.
Embodiment 23: compound red sage root spray
Get Radix Salviae Miltiorrhizae 400g, Radix Notoginseng 120g, Borneolum Syntheticum 6g gets red rooted salvia and pulverizes supercritical CO
2Extraction adds 40% medical material amount, 90% ethanol and makes entrainer, and 50 ℃ of extracting pressure 30MPa, temperature get red sage root super critical extract red sage root super critical extract 6.5g; Behind the supercritical extraction, medicated powder is taken out, decocting boils 2 times, add 4 times of water gagings respectively, decocted 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 750% ethanol elution reclaims ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 18g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 4.23g
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by, 10% tween 80 alcoholic solution 200ml adds 1,2-propylene glycol 100ml, mixing, ethanol is fixed molten to 1000ml, and the 50ml/ bottle is sub-packed in the aerosol pressure bottle, is pressed into dichlorodifluoromethane 6g behind the gland in every bottle, make spray, daily 10-15ml.
Embodiment 24: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 140g, Radix Notoginseng 28g, Borneolum Syntheticum 2g, behind the supercritical extraction, medicated powder is taken out, the medicated powder decocting boils 2 times, add 6,5,5 times of water gagings, decocted 1.5,1,1 hours, merge decocting liquid, concentrate, add 95% ethanol and make and contain alcohol amount and reach 60%, left standstill 12 hours, filter, the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 1.68g;
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized mix homogeneously; Press medicated powder: the adding medicament mixed was even with after substrate PEG6000 or the PEG400 fusion in 1: 1.8 for substrate, and 70 ℃ of insulations in 50 droplets/minute adding methyl-silicone oils, are collected drop pill, absorb liquid coolant with filter paper, make 2222 balls, the heavy 50mg of every ball, and day is obeyed 70 balls.
Embodiment 25: the compound Salviae Miltiorrhizae granule
Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g, the refining thing 1.68g of post, Borneolum Syntheticum 2g are crossed in the Radix Notoginseng alcohol extraction.
Get Radix Salviae Miltiorrhizae 140g, Radix Notoginseng 28g, Borneolum Syntheticum 2g, behind the supercritical extraction, medicated powder is taken out, the medicated powder decocting boils 2 times, add 6,5,5 times of water gagings, decocted 1.5,1,1 hours, merge decocting liquid, concentrate, add 95% ethanol and make and contain alcohol amount and reach 60%, left standstill 12 hours, filter, the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 1.68g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add the carboxymethyl starch sodium of 3 times of amounts of suitable medicine, make soft material, make granule 160g, each 5g, day clothes 1-2 time with the ethanol of 80% concentration.
Embodiment 26: FUFANG DANSHEN JIAONANG
Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g, the refining thing 1.68g of post, Borneolum Syntheticum 2g are crossed in the Radix Notoginseng alcohol extraction.
Get Radix Salviae Miltiorrhizae 140g, Radix Notoginseng 28g, Borneolum Syntheticum 2g, behind the supercritical extraction, medicated powder is taken out, the medicated powder decocting boils 2 times, add 6,5,5 times of water gagings, decocted 1.5,1,1 hours, merge decocting liquid, concentrate, add 95% ethanol and make and contain alcohol amount and reach 60%, left standstill 12 hours, filter, the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 1.68g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The carboxymethyl starch sodium that adds 1 times of amount of suitable medicine is made 300 of capsules, and each 3, day clothes 3 times.
Embodiment 27: compound red sage root spray
Get Radix Salviae Miltiorrhizae 140g, Radix Notoginseng 28g, Borneolum Syntheticum 2g, behind the supercritical extraction, medicated powder is taken out, the medicated powder decocting boils 2 times, add 6,5,5 times of water gagings, decocted 1.5,1,1 hours, merge decocting liquid, concentrate, add 95% ethanol and make and contain alcohol amount and reach 60%, left standstill 12 hours, filter, the filtrate concentrate drying gets Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract 36g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 1.68g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by; 15% tween 80 alcoholic solution 100ml, ethanol is fixed molten to 300ml, adds 1,2-propylene glycol 30ml, mixing, ethanol is fixed molten to 300ml, and the 60ml/ bottle is sub-packed in the aerosol pressure bottle, in every bottle, be pressed into dichlorodifluoromethane 1g behind the gland, make spray, daily 15ml.
Measure by embodiment 10 method of quality control: every milliliter of contained danshensu is no less than 0.28mg, and protocatechualdehyde is no less than 0.09mg.Every milliliter of contained Borneolum Syntheticum is no less than 3.6mg in Borneolum Syntheticum.Every milliliter of contained Radix Notoginseng is with the ginsenoside Rg
1Meter is no less than 0.71mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 0.76mg.
Embodiment 28: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 133g, Radix Notoginseng 26g, Borneolum Syntheticum 1.5g, the Radix Salviae Miltiorrhizae decocting boils 2 times, adds 4 times of water gagings respectively, decocts 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 75% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 8g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 1.56g;
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized mix homogeneously; Press medicated powder: the adding medicament mixed was even with after the substrate PEG4000 fusion in 1: 1.5 for substrate, and 80 ℃ of insulations in 60 droplets/minute adding methyl-silicone oils, are collected drop pill, absorb liquid coolant with filter paper, get drop pill 922 balls, the heavy 30mg of every ball, and day is obeyed 31 balls.
Pressing embodiment 10 method of quality control measures: the ginsenoside Rg
1With ginsenoside Rb
1The mobile phase of assay be 3: 7 second cyanogen-water, every contained danshensu of drop pill is no less than 0.07mg, protocatechualdehyde is no less than 0.023mg.Every contained Borneolum Syntheticum of drop pill is no less than 0.89mg in Borneolum Syntheticum.Every contained Radix Notoginseng of drop pill is with the ginsenoside Rg
1Meter is no less than 0.21mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 0.42mg.
Embodiment 29: the compound Salviae Miltiorrhizae granule
Get Radix Salviae Miltiorrhizae 133g, Radix Notoginseng 26g, Borneolum Syntheticum 1.5g, the Radix Salviae Miltiorrhizae decocting boils 2 times, adds 4 times of water gagings respectively, decocts 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 75% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 8g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 1.56g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add 4 times of amounts of suitable medicine-cyclodextrin, make soft material with the ethanol of 60% concentration, make granule 55g, each 2g, day clothes 1-2 time.
Embodiment 30: FUFANG DANSHEN JIAONANG
Get Radix Salviae Miltiorrhizae 133g, Radix Notoginseng 26g, Borneolum Syntheticum 1.5g, the Radix Salviae Miltiorrhizae decocting boils 2 times, adds 4 times of water gagings respectively, decocts 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 75% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 8g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 1.56g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add 2 times of amounts of suitable medicine-cyclodextrin, make 120 of granules, each 2, day clothes 2 times.
Embodiment 31: compound red sage root spray
Get Radix Salviae Miltiorrhizae 133g, Radix Notoginseng 26g, Borneolum Syntheticum 1.5g, the Radix Salviae Miltiorrhizae decocting boils 2 times, adds 4 times of water gagings respectively, decocts 1 hour, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 75% ethanol elution, reclaim ethanol, concentrate drying gets Radix Salviae Miltiorrhizae water and carried the refining thing 8g of post; Get pseudo-ginseng, be ground into coarse grain, decocting boils 3 times, add 6,5,4 times in water respectively, decocted 1.5,1,1 hours, merge decocting liquid, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, washing, abandon water lotion, 70% ethanol elution reclaims ethanol, concentrate drying gets Radix Notoginseng water extract 1.56g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by; Add 15% tween 80 alcoholic solution 100ml, ethanol is fixed molten to 300ml, adds 1,2-propylene glycol 30ml, mixing, ethanol is fixed molten to 300ml, and the 100ml/ bottle is sub-packed in the aerosol pressure bottle, in every bottle, be pressed into dichlorodifluoromethane 1g behind the gland, make spray, daily 10-15ml.
Embodiment 32: FUFANG DANSHEN DIWAN
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g
Get red rooted salvia and pulverize supercritical CO
2Extraction adds 40% medical material amount, 95% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 2.82g;
With Radix Salviae Miltiorrhizae extract, it is stand-by that refining thing of Radix Notoginseng and Borneolum Syntheticum are pulverized mix homogeneously; Press medicated powder: the adding medicament mixed was even with after the substrate PEG4000 fusion in 1: 1.5 for substrate, and 80 ℃ of insulations in 30 droplets/minute adding methyl-silicone oils, are collected drop pill, absorb liquid coolant with filter paper, make 1332 balls, the heavy 25mg of every ball, and day is obeyed 12 balls.
Embodiment 33: the compound Salviae Miltiorrhizae granule
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g
Get red rooted salvia and pulverize supercritical CO
2Extraction adds 40% medical material amount, 95% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 2.82g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; Add the dextrin of 4 times of amounts of suitable medicine, make soft material, make granule 70g, each 0.7g, day clothes 1-2 time with the ethanol of 60% concentration.
Embodiment 34: FUFANG DANSHEN JIAONANG
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g
Get red rooted salvia and pulverize supercritical CO
2Extraction adds 40% medical material amount, 95% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 2.82g;
Press recipe quantity with Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The dextrin that adds 2 times of amounts of suitable medicine is made 150 of granules, each 1-2 grain, day clothes 3 times.
Embodiment 35: the compound Salviae Miltiorrhizae soft capsule
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g
Get red rooted salvia and pulverize supercritical CO
2Extraction adds 40% medical material amount, 95% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 2.82g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously; The vegetable oil that adds 1.5 times of amounts of suitable medicine, even with the colloid mill mill, make 200 of soft capsules, each 1, day clothes 2 times.
Press embodiment 10 method of quality control and measure the ginsenoside Rg
1With ginsenoside Rb
1The amino normal phase column of assay, mobile phase is 87: 13 second cyanogen-1% phosphoric acid, every contained tanshinone of drop pill is no less than 2.25mg, every contained Borneolum Syntheticum of drop pill is no less than 16.5mg in Borneolum Syntheticum.Every contained Radix Notoginseng of drop pill is with the ginsenoside Rg
1Meter is no less than 5.35mg, with the ginsenoside Rg
1With ginsenoside Rb
1The total amount meter is no less than 10.7mg.
Embodiment 36: compound red sage root spray
Get Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Borneolum Syntheticum 6g
Get red rooted salvia and pulverize supercritical CO
2Extraction adds 40% medical material amount, 95% ethanol and makes entrainer, and 35 ℃ of extracting pressure 23MPa, temperature get red sage root super critical extract 4.5g; Get pseudo-ginseng, be ground into coarse grain, add 60% alcohol reflux 3 times, each 1 hour, extracting solution reclaimed ethanol, be concentrated into relative density and be 1-1.1, centrifugal, supernatant is crossed macroporous resin column, and water lotion is abandoned in washing, 60% ethanol elution reclaims ethanol, and concentrate drying gets Radix Notoginseng ethanol extract 2.82g;
With Radix Salviae Miltiorrhizae extract, Radix Notoginseng extract and Borneolum Syntheticum fine powder mix homogeneously are stand-by; Add 15% tween 80 alcoholic solution 120ml, add 1,2-propylene glycol 80ml, mixing, it is fixed molten to 800ml to add ethanol again, and the 100ml/ bottle is sub-packed in the aerosol pressure bottle, is pressed into dichlorodifluoromethane 4g behind the gland in every bottle, makes spray, daily 8-10ml.
Claims (7)
1, a kind of compound red sage root preparation is characterized in that said preparation made by following crude drug:
Radix Salviae Miltiorrhizae extract 4-60 weight portion, Radix Notoginseng extract 2-18 weight portion, Borneolum Syntheticum 1-10 weight portion;
Described Radix Salviae Miltiorrhizae extract is that Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water were carried refining thing of post and red sage root super critical extract or tanshinol extract by 80-20: the compositions of 20-80 percentage;
Described Radix Notoginseng extract is Radix Notoginseng water extract or Radix Notoginseng ethanol extract;
Described red sage root super critical extract or tanshinol extract contain tanshinone and are no less than 5-10%; Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water were carried the refining thing of post and are contained danshensu and be no less than 0.5-1.5%, and protocatechualdehyde is no less than 0.16-0.48%; Radix Notoginseng water extract or Radix Notoginseng ethanol extract contain the ginsenoside Rg
1Be no less than 15.83-30%, contain the ginsenoside Rg
1And Rb
1Be no less than 31-40%.
2, compound red sage root preparation as claimed in claim 1, it is characterized in that wherein said Radix Salviae Miltiorrhizae water extract-alcohol precipitation extract or Radix Salviae Miltiorrhizae water carried refining thing of post and red sage root super critical extract or tanshinol extractive composition percentage and be: 70-30: 30-70,60-40: 40-60 or 50: 50.
3, compound red sage root preparation as claimed in claim 1 is characterized in that said preparation made by following crude drug:
Radix Salviae Miltiorrhizae extract 36-54 weight portion, Radix Notoginseng extract 8.46-16.92 weight portion, Borneolum Syntheticum 2-8 weight portion.
4, compound red sage root preparation as claimed in claim 1 is characterized in that said preparation made by following crude drug:
Radix Salviae Miltiorrhizae extract 18-27 weight portion, Radix Notoginseng extract 4.23-8.46 weight portion, Borneolum Syntheticum 1-2 weight portion.
5, compound red sage root preparation as claimed in claim 1 is characterized in that said preparation made by following crude drug:
Radix Salviae Miltiorrhizae extract 4.5-9 weight portion, Radix Notoginseng extract 2.82-5.64 weight portion, Borneolum Syntheticum 4-6 weight portion.
6, as claim 1,3,4 or 5 described compound red sage root preparations, it is characterized in that Borneolum Syntheticum replaces with Camphora.
7,, it is characterized in that to add in the preparation adjuvant and make any dosage form in clinical middle tablet, capsule, oral liquid, drop pill, spray or the granule as the arbitrary described compound red sage root preparation of claim 1-5.
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| CN100404035C (en) * | 2003-09-23 | 2008-07-23 | 天津天士力制药股份有限公司 | Combination of Chinese traditional medicine for curing cardiovascular diseases and cerebrovascular disease |
| CN100391452C (en) * | 2003-12-15 | 2008-06-04 | 张正生 | Red sage root preparation for injection and its preparation method |
| CN100381133C (en) * | 2003-12-31 | 2008-04-16 | 天津天士力制药股份有限公司 | Application of pharmaceutical composition in the process for preparing medicine to treat aspirin resisting disease |
| CN101167838B (en) * | 2006-11-06 | 2010-12-01 | 张文武 | Traditional Chinese medicinal preparation for treating coronary heart disease and angina pectoris and its preparing process |
| CN101062088B (en) * | 2007-05-29 | 2011-06-08 | 北京宝泰宁堂生物技术有限公司 | Cardiac and cerebral vascular disease treating medicine, the preparing method, the quality control method and the function thereof |
| CN102526380B (en) * | 2010-12-28 | 2014-02-05 | 天津同仁堂集团股份有限公司 | Detection method of coronary artery dredging tablet |
| CN103432199A (en) * | 2013-08-27 | 2013-12-11 | 江西中医学院 | Preparation method of pharmaceutical composition, and pharmaceutical composition prepared thereby |
| KR20230088371A (en) * | 2021-09-27 | 2023-06-19 | 타슬리 파마슈티컬 그룹 컴퍼니 리미티드 | Pharmaceutical composition for treating myocardial ischemia and manufacturing method thereof |
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| CN1348815A (en) * | 2001-11-09 | 2002-05-15 | 天津天士力制药股份有限公司 | Medicine for preventing and treating coronary heart disease and angina pectoris and its prepn and other use |
| CN1349818A (en) * | 2001-10-24 | 2002-05-22 | 沈阳药科大学 | Effective part group in red sage mixture and its delayed release prepn. medicinal use and prepn process |
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| CN1349818A (en) * | 2001-10-24 | 2002-05-22 | 沈阳药科大学 | Effective part group in red sage mixture and its delayed release prepn. medicinal use and prepn process |
| CN1348815A (en) * | 2001-11-09 | 2002-05-15 | 天津天士力制药股份有限公司 | Medicine for preventing and treating coronary heart disease and angina pectoris and its prepn and other use |
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