CN1283402A - 赖氨酸碱干粉的生产方法和用途 - Google Patents
赖氨酸碱干粉的生产方法和用途 Download PDFInfo
- Publication number
- CN1283402A CN1283402A CN00122569A CN00122569A CN1283402A CN 1283402 A CN1283402 A CN 1283402A CN 00122569 A CN00122569 A CN 00122569A CN 00122569 A CN00122569 A CN 00122569A CN 1283402 A CN1283402 A CN 1283402A
- Authority
- CN
- China
- Prior art keywords
- methionin
- lysine
- feed
- alkali
- dry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004472 Lysine Substances 0.000 title claims abstract description 35
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 239000000843 powder Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 13
- 229930182817 methionine Natural products 0.000 claims description 59
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 claims description 58
- 239000003513 alkali Substances 0.000 claims description 35
- 238000001035 drying Methods 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 238000005507 spraying Methods 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 108010039918 Polylysine Proteins 0.000 claims description 2
- 229920000656 polylysine Polymers 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 27
- 229930195722 L-methionine Natural products 0.000 description 27
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 24
- 229960003646 lysine Drugs 0.000 description 20
- 235000018977 lysine Nutrition 0.000 description 18
- 239000000047 product Substances 0.000 description 17
- 241001465754 Metazoa Species 0.000 description 16
- 239000000654 additive Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 14
- 230000000996 additive effect Effects 0.000 description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 11
- 235000001014 amino acid Nutrition 0.000 description 10
- 229940024606 amino acid Drugs 0.000 description 10
- 150000001413 amino acids Chemical class 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000004584 weight gain Effects 0.000 description 5
- 235000019786 weight gain Nutrition 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 230000002349 favourable effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 235000019766 L-Lysine Nutrition 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 208000010444 Acidosis Diseases 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000370738 Chlorion Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 description 1
- 229930182818 D-methionine Natural products 0.000 description 1
- 239000004470 DL Methionine Substances 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010020597 Hyperchloraemia Diseases 0.000 description 1
- 241001071864 Lethrinus laticaudis Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 206010027417 Metabolic acidosis Diseases 0.000 description 1
- 241001467578 Microbacterium Species 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 238000003639 Student–Newman–Keuls (SNK) method Methods 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- -1 ammonia ester Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 150000003841 chloride salts Chemical group 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000002036 drum drying Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000005243 fluidization Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229960002989 glutamic acid Drugs 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229960005337 lysine hydrochloride Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 1
- 235000006109 methionine Nutrition 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229940029985 mineral supplement Drugs 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009645 skeletal growth Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229960002898 threonine Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/807—Poultry or ruminant feed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dermatology (AREA)
- Fodder In General (AREA)
Abstract
本发明涉及一种含有赖氨酸碱干粉的饲料或预混合料,其中干物质中的游离赖氨酸碱含量大于70%重量,以及赖氨酸碱干粉的生产方法及其用途。
Description
本发明涉及赖氨酸碱干粉在食品、药物制剂加工以及动物饲料领域的应用及其生产方法。
L-赖氨酸是一种必需氨基酸，广泛用作食品和饲料的添加剂，例如用于生产营养食品以及饲料。氨基酸如赖氨酸也广泛应用于医药，例如作为注射液的组分。L-赖氨酸促进，例如骨骼的生长以及刺激细胞分裂和核苷合成。
全世界合成的90％以上的L-赖氨酸都应用于生产饲料，特别是用于生产猪或家禽饲料。将L-赖氨酸用于生产饲料的目的是为了给家畜提供必需氨基酸，因为在家畜营养中常用蛋白质饲料中缺少L-赖氨酸。饲料中的赖氨酸没有限制，并且家畜的动物饲养性能明显提高。
目前主要使用的是L-赖氨酸一盐酸盐(L-赖氨酸HCl)，其中L-赖氨酸的含量约79％，它已采用来例如作为饲料的L-赖氨酸添加剂。赖氨酸盐酸盐的使用导致家畜饮水增加(US 4，919，945)。这常常造成没有用的“潮湿废料”出现并增加了家畜感染的危险。为减少吸湿产物的出现必须将大量的添加剂混合入浓缩物。然而，这对大多数情况下氨基酸含量已经相对较低的饲料来说会进一步降低氨基酸的含量。
此外，碱性氨基酸的氯化物盐形式有非常苦的味道，在饲料中更大量的供给，则会减少家畜对营养物质的摄取。
L-赖氨酸盐酸盐在药物注射液中的应用可能会引起高氯血代谢性酸中毒。如果这样的注射液应用于肾衰病人，由于氯离子的作用会对病人已经紊乱的电解质平衡造成进一步的负担。
现有技术(EP-B122163)教导可以在保持特定的发酵条件下获得粗发酵肉汤而后干燥成稳定的固体产品。然而这种通过将发酵肉汤完全脱水获得的产品中L-赖氨酸的含量仅有35-48％重量，因此明显少于L-赖氨酸一盐酸盐。
EP-B 533 039要求保护一种动物饲料添加剂，其中具有高含量的“氨基酸”并且还含有发酵肉汤中的绝大多数组分。根据公开的发酵方法获得的最终产品(参见实施例）中不是游离氨基酸而是氨基酸的盐形式，根据发酵条件，例如是硫酸盐或碳酸盐。
将发酵肉汤喷雾干燥而不再纯化。氨基酸的重量百分比，例如L-赖氨酸碱的含量，该专利中规定理论上是根据赖氨酸盐计算游离碱的含量。
生产的另一种产品形式是液体赖氨酸碱，其中除50％赖氨酸碱外，还含有发酵副产物并主要是水。由于其总体积大造成运输，保存，包装以及加工的高成本，液体赖氨酸碱的缺点是需要特别的使用技术并且生产中需要的产品浓度低。
针对干燥条件下许多形式的赖氨酸自身所具有的粘着性，现有技术中公开了特定的添加剂，例如CaSO4，用于降低粘着性(DE-A2447274)。
本发明的目的之一是提供一种具有高含量的游离L-赖氨酸并具有高营养价值的L-赖氨酸碱干粉，及其生产方法。要求最终的固体产品具有良好的储存和加工性能。此外，该产品的粘着性应很小，不需要为降低粘着性加入添加剂，这样则不会减少所需产品浓度。
我们发现本发明的目的通过包含赖氨酸碱干粉的饲料或预混合料而实现，其中干物质中游离赖氨酸碱含量大于70％重量。
同样可以使用赖氨酸盐(例如盐酸盐)产品，其具有极好的流动特性和很小的粘着性。也可以将其溶解在水中制成浓缩赖氨酸碱溶液。
通常产品中含有按重量计约2-3％的残存水份。
干物质中游离赖氨酸碱含量大于70重量％，优选大于90重量％。
生产L-赖氨酸碱干分的赖氨酸原料可以是，例如是可以通过酸解获得L-赖氨酸的蛋白质。D，L-赖氨酸可以通过比学合成获得，例如通过D，L-α氨基-ε-己内酰安合成以及L-赖氨酸和D-赖氨酸也可以通过外消旋物的拆分获得。
通常L-赖氨酸是通过微生物，特别是棒状杆菌属、短杆菌属、节细菌属、微杆菌属、芽胞杆菌属或诺卡氏菌属发酵并随后进行纯化，优选离子交换剂纯化获得。包括直接或除去生物量后将发酵肉汤放于离子交换剂上。微生物学方法生产L-赖氨酸，例如公开在Trends Biotechnology 1983，1，70-74。
新方法的区别在于干物质中游离赖氨酸喊含量大于70重量％，优选大于90重量％。
然后使用常规浓缩和／或干燥方法如喷雾干爆，流化式喷雾干燥，转鼓式干燥或冷冻干燥方法将新的赖氨酸喊干粉转化成粉剂。优选的干燥方法是喷雾干燥(EP-A0457075；EP-A-O497177)，其中温度是80-200℃，优选110-170℃。
术语赖氨酸碱是指赖氨酸，优选游离形式的L-赖所氨酸(H2N-(CH2)4-CHNH2-CO2H；pK～10．5)。
除含有游离赖氨酸碱(70-97％重量)，干物质中还可以包括来自发酵培养基的少量其它氨基酸或蛋白质。
赖氨酸碱干粉在食品工业中的新应用是有利的，优选在家畜营养学，特别优选作为猪，仔猪和家禽如蛋鸡，肉鸡或火鸡的饲料添加剂。为此目的可以将他们加入到所有的常规家畜饲料如化合物饲料和／或单一成分饲料如禾谷类如小麦，燕麦，黑麦，大麦和豆科植物，玉米或玉米棒子芯混合物中。使用前将赖氨酸碱干粉直接或通过与其它饲料添加剂混合好的预混合料的形式加入到饲料中。
在赖氨酸碱干粉中可以加入的助剂是农业上使用的所有常规助剂，例如调味剂，着色剂，促进食欲的物质，抗菌素，前抗生素(probiotics)和／或酶。加入助剂量为赖氨酸碱干粉重量的0．1％-50％是有利的，优选按重量计0．1-10％。
根据饲料组成确定赖氨酸碱干粉的不同使用量是有利的。通常每公吨家畜饲料加入0．1-10kg赖氨酸添加剂是足够的。优选每公吨家畜饲料中加入0．5-5kg的赖氨酸碱。
赖氨酸碱干粉可根据已知方法用于生产饲料或其混合物。可以在得到或储存后立即加入饲料中。固体或液体赖氨酸碱可以直接或以预混合料的方式加入饲料中，在饲料的造粒或挤压过程中加入是有利的，例如通过计量装置。
赖氨酸碱干粉也可以用于药物或化妆品生产中。
本发明新的赖氨酸碱干粉的另一个用途是用于聚赖氨酸工业合成。
根据以下实施例对本发明作详细说明。
实施例1：通过发酵方法生产L-赖氨酸
使用US 3，708，395中的谷氨酸棒状菌ATCC21526，以常规方法进行赖氨酸发酵(72小时)后，发酵肉汤用H2SO4酸化至pH2，并用离子交换剂NH3稀释，真空下除去NH3，而后浓缩成68％的干物质。该浓缩物用于喷雾干燥方法。
实施例2：L-赖氨酸喷雾干燥
将实施例1的浓缩物在下面条件下喷雾干燥：
带有喷雾器的喷雾干燥器
·入口温度165℃
·出口温度110℃
·喷雾器速度10，000rpm(喷雾干燥器Niro Production minor)
以这种方式干燥后的产品含有97％干物质并且赖氨酸碱的含量为91．8％。而且没有粘着性易于生产。
实施例3：在赖氨酸碱饲喂仔猪饲养试验中90％赖氨酸碱利用率
该试验中使用断奶器目的是测试与Sewon L-赖氨酸HCl99％饲料(79％L-赖氨酸)相比90％赖氨酸碱产品的利用率。试验产品是喷雾干燥的含有90％L-赖氨酸活性物质的赖氨酸碱。
将总共45头(GLxP)生下28天的并且平均体重7．2kg的仔猪进行49天的饲喂试验。动物是来自一胎生下的9头小猪并根据block-formation规则随机分成5组处理。对以下各组处理进行测定：
| 组 | 赖氨酸添加剂％ | 测试物质 |
| Ⅰ | - | - |
| Ⅱ | 0．12 | 0．152％L-赖氨酸HCl |
| Ⅲ | 0．24 | 0．304％L-赖氨酸HCl |
| Ⅳ | 0．12 | 0．133％90％赖氨酯碱 |
| Ⅴ | 0．24 | 0．266％90％赖氨酸碱 |
组Ⅰ作为阴性对照并给以没有赖氨酸添加剂的基本饲料。在组Ⅱ和Ⅲ中，分别含有0．12％和 0．24％的赖氨酸，以Sewon L-赖氨酸HCl99％商品饲料形式(最少79％L-赖氨酸，BASF Aktiengesellschaft Ludwigshafen)加入基本饲料中。在组Ⅳ和Ⅴ中，添加剂是与测试物质90％赖氨酸碱形式等量的赖氨酸。在下表1中可发现使用了低含量赖氨酸基本饲料组分。基本饲料含有0．79％赖氨酸并预计可满足仔猪约60％的赖氨酸需求。所有其它种类氨基酸含量满足要求。除赖氨酸外，饲料符合用于仔猪饲养的标准Ⅱ型饲料的要求。
家畜分别放在可调节空气的试验房圈中。在试验过程中将室温从26℃逐渐降低至22℃,相对湿度约55％。可随意提供丸状饲料。对没有吃掉的饲料一周重新称重两次。每周测量家畜的体重。试验参数是体重增重，饲料消耗量和饲料转化率。
试验数据进行方差分析并采用F测验测定其显著差异，采用Student／Newman-Keuls测验比较每组的平均值。每组平均值之间的显著差别用不同的上标标记。
重量变化，每日增重，饲料消耗量和饲料转化率的结果汇总在表2中。组Ⅰ中家畜的重量变化，饲料消耗量和饲料转化率明显比添加有赖氨酸产品的组Ⅱ-Ⅴ中的家畜差。因此，基本饲料中赖氨酸的供给是明显缺少的。
赖氨酸HCl形式的0．12％和0．24％赖氨酸添加剂的日增重由209g／d分别显著增加至312和349g,与组Ⅰ比多49％和67％。以赖氨酸碱形式相应添加的赖氨酸使日增重分别提高至308和355g，即与阴性对照(组Ⅰ)相比多47％和70％。因此赖氨酸产品和赖氨酸添加剂在促进生长上是差不多的。
饲料的消耗量随赖氨酸供给量的增加同样明显变化。以赖氨酸HCl形式添加0．12％和0．24％赖氨酸时，与对照组动物比较。仔猪消耗的饲料量证实分别增加了27％和 30％。在相应的赖氨酸碱添加剂的组Ⅳ和组Ⅴ中动物的饲料消耗量分别是25％和33％，高于组Ⅰ。因此两种赖氨酸形式的饲料消耗量的类似增加，也表明赖氨酸碱是很适合的。
与阴性对照相比，以赖氨酸HCl和赖氨酸碱形式添加0．12％和0．24％赖氨酸，饲料转化率分别显著提高了15％和22％。对于两种赖氨酸形式，它们的添加剂含量的效果也被证实。因此两种赖氨酸产品在饲料转化中表现了相当的效果。
该试验表明当添加等量赖氨酸进行比较时，试验产品90％赖氨酸碱的生物效率类似于标准产品赖氨酸HCl。这适用于所有测试的动物饲养学参数：日增重，饲料消耗量和饲料转化率。试验结果表明新开发的产品90％赖氨酸碱至少与标准产品赖氨酸HCl的效果相当。表1：使用的基本饲料组成
表2：L-赖氨酸HCl99％和90％赖氨酸碱对断奶幼畜体重、日增重、日饲料消耗量和饲料转化的效果
| 组分 | ％ |
| 小麦 | 40．00 |
| 玉米 | 18．12 |
| 大麦 | 6．00 |
| 提取大豆粉 | 14，15 |
| 玉米面筋 | 10．00 |
| 肉／骨粉 | 3．00 |
| 小麦 | 2．10 |
| 苜蓿粉 | 2．00 |
| 豆油 | 2．00 |
| DL-蛋氨酸 | 0．05 |
| L-苏氨酸 | 0．08 |
| 矿物质，维生素和微量元素添加剂 | 2．50 |
| 粗蛋白 | 17．70 |
| 代谢能量，MJ／kg | 13．10 |
| 钙 | 0．90 |
| 磷 | 0．70 |
| 组添加赖氨酸，％赖氨酸类型 | -- | 0．12赖氨酸HCl | 0．24赖氨酸HCl | 0．12赖氨酸碱 | 0．24赖氨酸碱 |
| 开始时重量，Kg | 7．19±0．92 | 7．21±0．93 | 7．20±0．72 | 7．19±0．72 | 7．20±0．99 |
| 结束时重量，kg相对％ | 18．51b±3．98100 | 22．50a±2．87122 | 24．30a±3．12131 | 22．28a±4．16120 | 24．59a±2．28133 |
| 增重，g／d相对，％ | 209b±66100 | 312a±44149 | 349a±38167 | 308a±50147 | 355a±72170 |
| 饲料消耗量，g／d相对，％ | 465b±121100 | 592a±83127 | 606a±64130 | 583a±107125 | 619a±99133 |
| 饲料转化相对，％ | 2．23a±0．15100 | 1．90b±0．0985 | 1．74c±0．1178 | 1．89b±0．0885 | 1．75c±0．1278 |
Claims (8)
1．一种含有赖氨酸碱干粉的饲料或预混合料，其中干物质中的游离赖氨酸碱含量大于70重量％。
2．根据权利要求1的饲料或预混合料，其中干物质中的游离赖氨酸碱含量大于90重量％。
3．一种赖氨酸碱干粉的生产方法，其中包括进行干燥以使干物质中游离赖氨酸碱含量大于70重量％。
4．根据权利要求3的生产方法，其中将赖氨酸发酵溶液进行提纯，然后不用进行中和或形成盐就将赖氨酸发酵溶液喷雾干燥为游离碱。
5．根据权利要求4的生产方法，其中赖氨酸发酵溶液用离子交换剂提纯，然后不用进行中和或形成盐就将赖氨酸发酵溶液喷雾干燥为游离碱。
6．根据权利要求4或5中任一项的方法，其中干物质中的游离赖氨酸碱含量大于90重量％。
7权利要求4-6中任一项方法生产的喷雾干燥赖氨酸碱干粉在动物饲料或预混合料的生产或添加中的应用。
8．权利要求4-6中任一项方法生产的喷雾干燥赖氨酸碱干粉在聚赖氨酸工业合成中作为起始物料的应用。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19932059A DE19932059A1 (de) | 1999-07-12 | 1999-07-12 | Verfahren zur Herstellung und Verwendung von Lysinbase-Trockenpulvern |
| DE19932059.4 | 1999-07-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1283402A true CN1283402A (zh) | 2001-02-14 |
| CN1250102C CN1250102C (zh) | 2006-04-12 |
Family
ID=7914224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001225693A Expired - Lifetime CN1250102C (zh) | 1999-07-12 | 2000-07-12 | 赖氨酸碱干粉的生产方法和用途 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6468580B1 (zh) |
| EP (1) | EP1068804A1 (zh) |
| KR (1) | KR20010049755A (zh) |
| CN (1) | CN1250102C (zh) |
| DE (1) | DE19932059A1 (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003219807A (ja) | 2002-01-25 | 2003-08-05 | Ajinomoto Co Inc | Ｌ−リジンを主成分とする造粒乾燥物 |
| WO2009106914A1 (en) * | 2008-02-29 | 2009-09-03 | Ajinomoto Eurolysine S.A.S. | Mixed aqueous solution of l-lysine and l-threonine |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU502019A1 (ru) * | 1973-10-11 | 1976-02-05 | Институт Биохимии Им. А.Н.Баха Ан Ссср | Штамм |
| FR2464032A1 (fr) * | 1979-08-29 | 1981-03-06 | Rhone Poulenc Ind | Nouvelles compositions a base de lysine, pour alimentation animale, et leur preparation |
| DE3242501C1 (de) | 1982-11-18 | 1984-03-29 | Degussa Ag, 6000 Frankfurt | Verwendung von waessrigen L-Lysin-Loesungen zur Supplementierung von Futtermitteln mit L-Lysin |
| FR2541867B1 (fr) | 1983-03-03 | 1987-06-05 | Aec Chim Organ Biolog | Nouvelles compositions pour alimentation animale a base de lysine et leur preparation |
| FR2576753B1 (fr) * | 1985-02-01 | 1990-04-13 | Aec Chim Organ Biolog | Nouvelles compositions pour l'alimentation des animaux |
| US4835309A (en) * | 1988-04-14 | 1989-05-30 | Eastman Kodak Company | Ion exchange recovery of L-lysine |
| DE4014262A1 (de) | 1990-05-04 | 1991-11-07 | Basf Ag | Gut rieselfaehige, nichtstaubende bindemittelfreie riboflavinspruehgranulate oder -mikrogranulate aus reinem riboflavin und ein verfahren zu deren herstellung |
| DK0497177T3 (da) | 1991-01-28 | 1995-04-10 | Hoffmann La Roche | Vitaminholdige formuleringer og fremstilling deraf |
| DE4130868C2 (de) | 1991-09-17 | 1994-10-13 | Degussa | Tierfuttermittelsupplement auf der Basis einer Aminosäure und Verfahren zu dessen Herstellung |
| US5990350A (en) * | 1997-12-16 | 1999-11-23 | Archer Midland Company | Process for making granular L-lysine |
| CA2255130A1 (en) * | 1997-12-16 | 1999-06-16 | Archer Daniels Midland Company | Process for making granular l-lysine feed supplement |
-
1999
- 1999-07-12 DE DE19932059A patent/DE19932059A1/de not_active Withdrawn
-
2000
- 2000-06-14 EP EP00112564A patent/EP1068804A1/de not_active Withdrawn
- 2000-06-23 US US09/602,320 patent/US6468580B1/en not_active Expired - Lifetime
- 2000-07-11 KR KR1020000039489A patent/KR20010049755A/ko not_active Application Discontinuation
- 2000-07-12 CN CNB001225693A patent/CN1250102C/zh not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1250102C (zh) | 2006-04-12 |
| DE19932059A1 (de) | 2001-01-18 |
| US6468580B1 (en) | 2002-10-22 |
| EP1068804A1 (de) | 2001-01-17 |
| KR20010049755A (ko) | 2001-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI110378B (fi) | Fermentointiliemipohjainen aminohappoeläinrehulisäosa, menetelmä sen valmistamiseksi ja sen käyttö | |
| Little et al. | Nutritional significance of soluble nitrogen in dietary proteins for ruminants | |
| JPH11504818A (ja) | 動物用飼料添加物および前記添加物を含む飼料 | |
| EP1003384A1 (en) | Granular plasma protein supplement with increased bio-efficacy | |
| US4600586A (en) | Livestock feed lot adaptation composition and method | |
| CN106578529A (zh) | 一种改善仔猪生产性能、肠道健康和抗氧化性能的组合物及其制备方法 | |
| EP0826310A1 (en) | FEED COMPOSITION CONTAINING POLY-$g(g)-GLUTAMIC ACID | |
| US8178088B2 (en) | Antiprotozoal composition | |
| CN1250102C (zh) | 赖氨酸碱干粉的生产方法和用途 | |
| US4073884A (en) | Promotion of feed efficiency | |
| US4235878A (en) | Animal feeds containing a mixture of avoparcin or spiramycin and proteolytic enzymes | |
| US6858239B2 (en) | Feed additive and method for controlling large bowel fermentation in the horse and similar animals | |
| Bercovici et al. | Industrial amino acids in nonruminant animal nutrition | |
| US4242363A (en) | Urea-based feed supplement | |
| JP2001029022A (ja) | 嗜好性向上ペットフード用組成物 | |
| US20220217999A1 (en) | Method for protecting protein from heat damage and reducing rumen degradability of methionine | |
| RU1790381C (ru) | Кормова добавка дл тел т | |
| RU689011C (ru) | Кормовой препарат витамина РР дл животных | |
| Parsons et al. | Utilization of amino acid analogues in diets of young turkeys | |
| CN117987283A (zh) | 采食调控酵母组合物、含有其的饲料和其应用 | |
| JPH03277240A (ja) | 飼料 | |
| CN112931699A (zh) | 一种过瘤胃氨基酸及其制备方法 | |
| RU1782521C (ru) | Кормова смесь дл цыпл т-бройлеров на заключительном этапе откорма | |
| Parsons et al. | Relative lysine potencies of eight analogues in diets of young turkeys | |
| Bush | Torula yeast in dairy cattle rations |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WHITE LIGHT INDUSTRY CO., LTD. Free format text: FORMER OWNER: BASF COMPANY Effective date: 20080613 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20080613 Address after: Jeonbuk, South Korea Patentee after: Paik Kwang Ind Co.,Ltd. Address before: Ludwigshafen, Federal Republic of Germany Patentee before: BASF AG |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160408 Address after: Seoul, South Kerean Patentee after: Daesang Co., Ltd. Address before: Jeonbuk, South Korea Patentee before: Paik Kwang Ind Co.,Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20060412 |
|
| CX01 | Expiry of patent term |