CN1271211C - Method and device for detecting biological cell - Google Patents
Method and device for detecting biological cell Download PDFInfo
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- CN1271211C CN1271211C CN 02152595 CN02152595A CN1271211C CN 1271211 C CN1271211 C CN 1271211C CN 02152595 CN02152595 CN 02152595 CN 02152595 A CN02152595 A CN 02152595A CN 1271211 C CN1271211 C CN 1271211C
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Abstract
The present invention relates to a method and a device for detecting biological cells, which uses a traditional cell article-laying stand, the cell article-laying stand is provided with a plurality of through holes which are arranged, and each of the through holes is provided with two electrodes made of metal materials. When the cells fall into the through holes, the bodies of the cells contact the two electrodes, and the bodies of the cells have impedance, electrical inductance or the properties of the current conduction of capacitance; thereby, when the two electrodes have a current or a voltage, the current or electrical fields can flow across the cells from one electrode to the other electrode; when the circulation of the cells has electrical specification, the cells present existence or physical reaction.
Description
Technical field
The present invention relates to a kind of biomass cells detection method and device, especially refer to utilize the method for physical property that the animal and plant cell is applied electric current, add impedance, inductance and the electric capacity matter characteristic of cell own, the cell body alive or electric field after, can learn whether cell exists or allow cell present the reaction of rerum natura in the perforation of a plurality of arrangements.
Background technology
Biotechnology is to utilize biomass cells or its metabolic substd to manufacture a product, or the technology that improvement is moved, planted, microorganism and related products thereof improve human lives's quality.This technology application industry scope has medicine, food, specialization, environmental protection, ocean, the energy and agricultural.In order to adapt to the needs of biotechnology research and development, gene mapping sequencing for example, the biochemical reaction of research process needs a large amount of and rapidly, " biochip " therefore arises at the historic moment.So-called biochip is the integration of functional living being material and micro-electromechanical technology, and the micro-electromechanical technology expection can provide the fine planning and design of chip, and the reacting environment of a plurality of fine zones as the functional living being material can be provided in the small area scope.
And when on biochip, biomass cells do being detected in the past, be chemically to detect, allow biomass cells directly fall into the perforation of cell rack (perforated cell carrier), after splashing into chemical agent again, add fluorescent agent, utilize the optics framework to observe the reaction of biomass cells simultaneously.This kind utilizes the detection method of chemical mode, and it is trifling not only to detect step, and employed peripherals is also more, and when utilizing chemical mode to detect, this chemical agent destructible tissue of biological cells causes the biomass cells shelf time shorter.
Summary of the invention
Main purpose of the present invention is to solve the defective of above-mentioned prior art, avoids defective to exist, and the present invention utilizes a kind of new detection method and device to change traditional chemical process of utilizing to understand the characteristic (rerum natura) of its cell (animal and plant cell).This novel method is to utilize a physical method to have the cell of inductance or electric capacity matter characteristic at some, does not need to dose fluorescent agent when detecting again, and can learn the cell response rerum natura, can also save the optics framework simultaneously.Thereby it is convenient easy that biomass cells is detected.
For realizing above-mentioned purpose, this novel method is to utilize the conventional cell rack, the perforation (hole) that has a plurality of arrangements on this cell rack, in perforation, be provided with two electrodes that constitute by metal material, the laying of this two electrode extends perforated outer by perforation inside, when cell falls into aforesaid perforation, this this cognition of cell contacts with two electrodes, add the cell body and have impedance, the conductive characteristic of inductance or electric capacity matter, therefore alive or during voltage at two electrodes, this electric current or electric field can be by electrode stream through cell to another electrode, when stream of cells is connected with electrical specification, need not add fluorescent agent, can learn whether cell exists or allow cell present rerum natura reaction in the perforation of a plurality of arrangements, to reach the purpose of utilizing physical method to detect cell.
Description of drawings
Relevant detailed content of the present invention and technical specification, existing conjunction with figs. is described as follows.
Fig. 1-1 is a cell detection rack synoptic diagram of the present invention.
Fig. 1-2 is the local enlarged diagram of Fig. 1-1.
Fig. 1-3 is that Fig. 1-2 is in A-A position section cross-sectional schematic.
Fig. 2 is a second embodiment of the present invention synoptic diagram.
Fig. 3 is a third embodiment of the present invention synoptic diagram.
Label declaration in the accompanying drawing
The cell rack ... ... ..1
Perforation ... ... ... ..11
Two electrodes ... ... ... 12,12 '
Cell ... ... ... ..2
Heating unit ... ... ... .3
Sensing apparatus ... ... ... .4
Embodiment
Referring to Fig. 1-1,1-2,1-3, be cell detection rack of the present invention and local enlarged diagram.As shown in the figure: cell detection method of the present invention and device mainly are to change tradition with new detection method and device to utilize chemical process to understand the characteristic (rerum natura) of its cell (animal and plant cell).This novel method is to utilize a physical method to have the cell of inductance or electric capacity matter characteristic at some, does not need to dose fluorescent agent when detecting again, and can learn the cell response rerum natura, can also save the optics framework simultaneously.Thereby it is convenient easy that biomass cells is detected.
The above-mentioned novel method of carrying is to utilize conventional cell rack (perforated cellcarrier) 1, the perforation (hole) 11 that has a plurality of arrangements on this cell rack 1, these 11 inside of boring a hole are the state of convergent from top to bottom, and in perforation 11, be provided with two electrodes 12 that constitute by metal material, 12 ', this two electrode 12,12 ' laying extends perforation 11 outsides by perforation 11 inside, when cell 2 falls into aforesaid perforation 11,2 cognition of this cell and two electrodes 12,12 ' is close or contacts, add cell 2 bodies and have impedance, the conductive characteristic of inductance or electric capacity matter, therefore at two electrodes 12,12 ' when being connected with direct current or alternating current or voltage, this electric current or electric field can be flowed through cell 2 to another electrode 12 ' by electrode 12, when cell 2 circulations have electrical specification, need not add fluorescent agent, can detect cell 2 and whether exist or allow cell 2 present rerum natura reaction, to reach the purpose of utilizing physical method to detect cell 2.
Simultaneously, utilize the physical method pair cell 2 of above-mentioned electrical specification to detect, need not assist down by the optics framework, can learn the rerum natura reaction of cell 2, can reduce the utilization of optics framework significantly.And, when utilizing physical method pair cell 2 detections of electrical specification, can not destroy the tissue of cell 2, make the shelf time of cell 2 longer.
Referring to Fig. 2, be second embodiment of the present invention synoptic diagram.As shown in the figure: the present invention carries out the physical detection methods of electrical specification biomass cells 2 except setting up two electrodes 12,12 ' in the perforation 11 of conventional cell rack 1, simultaneously, can also be formed with a heating unit (nichrome wire) 3 in perforation 11 inside with electrode 12,12 ' mode, after allowing cell 2 fall into perforation 11 inside of cell rack 1, except but pair cell 2 applies the electrical specification of electric current, also pair cell 2 heats simultaneously, allow cell 2 except reaction, also have the reaction after the heating with current flowing.
Referring to Fig. 3, be third embodiment of the present invention synoptic diagram.As shown in the figure: present embodiment and the above embodiments are roughly the same, different being in this heating unit 3 be located on the periphery of perforation 11, on cell rack 1, also set up simultaneously a sensing apparatus 4, this sensing apparatus 4 is in order to the Heating temperature of sensing cell 2, avoid over-heating and cause cell 2 necrosis, pair cell 2 detects effectively.
Further, the above-mentioned heating unit of carrying can also heat for the cell 2 on the external heated equipment pair cell rack 1 except can be nichrome wire.
Further, sensing apparatus on can being located at cell rack 1, also can be constituted by the sensor device of outside.
Above-mentioned is preferred embodiment of the present invention only, is not to be used for limiting scope of the invention process.Be that all equalizations of doing according to claim of the present invention change and modification, be claim of the present invention and contain.
Claims (10)
1. a cell detection device is the device that utilizes the physical property pair cell to detect, and it is characterized in that this device includes:
The cell rack (1) of one ccontaining cell (2) has the perforation (11) of a plurality of arrangements on it;
One is located at above-mentioned cell rack (1) goes up and extends the inner electrode (12,12 ') of perforation (11);
Whereby, biomass cells (2) fall into perforation (11) and electrode (12,12 ') near to or in contact with, alive or during voltage at electrode (12,12 '), this electric current or electric field can flow through biomass cells (2), can learn whether cell (2) exists in the perforation (11) of a plurality of arrangements or allow biomass cells (2) present rerum natura reaction.
2. cell detection device according to claim 1, this electrode (12,12 ') has two electrode characteristics, to be connected with direct current or alternating current or voltage.
3. cell detection device according to claim 1, this device also are provided with a heating unit (3) of being located on the cell rack (1).
4. cell detection device according to claim 3, this heating unit (3) can be located at cell rack (1) and go up and extend in the perforation (11).
5. cell detection device according to claim 3, this heating unit (3) can be nichrome wire.
6. cell detection device according to claim 3, this heating unit (3) can be formed by indirect heating equipment.
7. cell detection device according to claim 1, this device also are provided with a sensing apparatus (4) of being located on the above-mentioned cell rack (1).
8. cell detection device according to claim 7, this sensing apparatus (4) is a temperature-sensing device.
9. cell detection device according to claim 7, this sensing apparatus (4) can be made of the sensor device of outside.
10. method of utilizing physical property pair cell (2) to detect with the described cell detection device of claim 1, this method includes:
A) utilize cell rack (1), have the perforation (11) of a plurality of arrangements on this cell rack (1), this perforation (11) is provided with two electrodes (12,12 ') that are made of metal material;
When b) treating that cell (2) falls into aforesaid perforation (11), this cognition of this cell (2) is close with two electrodes (12,12 ') or contacts, add the conductive characteristic that cell (2) body has impedance, inductance or electric capacity matter, therefore at two electrodes (12,12 ') when aliving, this electric current or electric field can be flowed through cell (2) to another electrode (12,12 ') by electrode (12,12 '), when cell (2) circulation has electrical specification, can allow cell (2) present the rerum natura reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02152595 CN1271211C (en) | 2002-11-29 | 2002-11-29 | Method and device for detecting biological cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02152595 CN1271211C (en) | 2002-11-29 | 2002-11-29 | Method and device for detecting biological cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1504578A CN1504578A (en) | 2004-06-16 |
| CN1271211C true CN1271211C (en) | 2006-08-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02152595 Expired - Fee Related CN1271211C (en) | 2002-11-29 | 2002-11-29 | Method and device for detecting biological cell |
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| Country | Link |
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| CN (1) | CN1271211C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101654651B (en) * | 2008-08-22 | 2012-07-04 | 上海电机学院 | Device and method for detecting state of anchorage-dependent cell in culture dish |
| FR3042873A1 (en) | 2015-10-23 | 2017-04-28 | Ecole Polytech | METHOD AND SYSTEM FOR DISCRIMINATING CELLS |
| US11568990B2 (en) | 2016-11-21 | 2023-01-31 | Sensome SAS | Characterizing and identifying biological structure |
| TWI662123B (en) | 2018-05-09 | 2019-06-11 | 國立清華大學 | Method of detecting cells |
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2002
- 2002-11-29 CN CN 02152595 patent/CN1271211C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1504578A (en) | 2004-06-16 |
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Granted publication date: 20060823 Termination date: 20091229 |