CN101654651B - Device and method for detecting state of anchorage-dependent cell in culture dish - Google Patents
Device and method for detecting state of anchorage-dependent cell in culture dish Download PDFInfo
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- CN101654651B CN101654651B CN 200810041988 CN200810041988A CN101654651B CN 101654651 B CN101654651 B CN 101654651B CN 200810041988 CN200810041988 CN 200810041988 CN 200810041988 A CN200810041988 A CN 200810041988A CN 101654651 B CN101654651 B CN 101654651B
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- petridish
- electrode
- mcu unit
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The invention relates to a device and a method for detecting the state of an anchorage-dependent cell in a culture dish. The device comprises a host computer and the culture dish, wherein the host computer comprises a mainboard, a button keyboard, a display module, a cabinet, a cable and a port support; and the mainboard is provided with an MCU unit, a storage unit, a clock module, a temperature measuring bridge, a waveform generator, a voltage regulation module, a button interface, a display drive and a 232 chip. The invention also provides a method for detecting the state of the anchorage-dependent cell in the culture dish. The device and the method have the advantages that: the device can perform online measurement in real time, and thus the device can be formed into an automatic culture device with other instruments; and the measurement is independent of cell overlapping and the measurement range can be completed within four orders of magnitude of cell concentration change, so the device and the method have wide measurement threshold.
Description
[technical field]
The present invention relates to a kind of device, relate in particular to a kind of devices and methods therefor that detects state of anchorage-dependent cell in the petridish.
[background technology]
Using petridish cultivation attached cell to be widely used in all kinds of breadboard small-scales in field such as biology, medical science, pharmacy, criminal investigation, inspection and quarantine spreads cultivation.Attached cell is cultivated normally one of modal experimental project in these laboratories, and it also is the most conventional, the basic experimental implementation in these laboratories that attached cell is cultivated operation.And counting, the volume of attached cell are assessed is one of substance of these experimental implementation.
At present, nearly all be manual method to the number of attached cell in the petridish, the assessment of volume, operation steps comprises the microscope qualitative evaluation, disappear wash, dilution, quantitative sampling, dyeing, microscopic counting etc.Measuring process is loaded down with trivial details, it is long to last, error is bigger, and prior problem is cultivation, the Monitoring systems that can not form robotization.
Now, the bacterium colony analyser of robotization occurs, and it is sent to computingmachine through CCD with the image on the microscopically petridish, and pattern recognition, analysis software in the computingmachine are analyzed the cell situation on the petridish automatically.It is actually the observation of manual work at microscopically is replaced with computingmachine; But this can not solve the associated problem that the microscope direct observing petridish is produced, as because of the counting of cell overlap is inaccurate, cell distribution uneven make view-point lack representative, dead cell also be calculated in interior, can not when cultivating, monitor etc.
One Chinese patent application: 200510118968.5 disclose a kind of Microstress applicator in vitro cell.One Chinese patent application: 200610020150.4 disclose a kind of cytoanalyze, but also do not appear in the newspapers at present about the device and the detection method thereof that detect state of anchorage-dependent cell in the petridish.
[summary of the invention]
The objective of the invention is, a kind of device that detects state of anchorage-dependent cell in the petridish is provided.
One purpose more of the present invention is that a kind of method of utilizing said apparatus to detect state of anchorage-dependent cell in the petridish is provided.
For realizing above-mentioned purpose; The technical scheme that the present invention takes is: a kind of device that detects state of anchorage-dependent cell in the petridish; Comprise main frame, petridish; Described main frame comprises mainboard, button keyboard, display module, casing, cable and port frame, and described mainboard is provided with MCU unit, storage unit, clock module, bridge for measuring temperature, waveform generator, Voltage stabilizing module, push-button interface, display driver, 232 chips
Described storage unit is connected with the MCU unit;
Bridge for measuring temperature is connected with the MCU unit;
Clock module is connected with the MCU unit;
The MCU unit is connected with wave filter through the A/D modular converter, and wave filter is connected with electrode in the petridish through magnifying glass, cable, port frame;
The MCU unit is connected with probe through waveform generator, IT module successively;
The MCU unit connects Voltage stabilizing module, and Voltage stabilizing module connects battery or electric main through power interface;
The MCU unit connects button keyboard through push-button interface;
The MCU unit connects display module through display driver;
The MCU unit connects 232 chips, and 232 chips connect 232 interfaces.
Optional, scribbling electrode on the described petridish bottom interior surface, described electrode is by two or four electrodes of painting with conductive silver glue.
Optional, described electrode is straight line or annulus lines, straight line lines electrode is left-right symmetric and distributes loop line strip electrode and petridish concentric.
Optional, comprise base, upper arm, interior electrode catheter, outer electrode probe, probe draw-in groove, port frame outlet line on the described port frame.
Optional, described base, upper arm are processed by tetrafluoroethylene integral body.
Optional, described upper arm surface is provided with the probe draw-in groove, electrode catheter, outer electrode in described probe draw-in groove is fixing.
Optional, described MCU unit is a dsp chip, waveform generator is a Direct Digital Frequency Synthesizers.
Optional, described dsp chip is selected the TMS32C2812 chip for use, and Direct Digital Frequency Synthesizers is selected the AD9834 chip for use.
Optional, described storage unit is RAM and FLASH.
For realizing above-mentioned second purpose, the technical scheme that the present invention takes is: a kind of method that detects state of anchorage-dependent cell in the petridish comprises following key step:
A, conductive silver glue is coated in the petridish internal surface by electrode desired shape and position, again with petridish sterilization, nutrient solution soak, sterilization, dry for standby again;
B, bacterium to be inoculated is introduced a fine variety the petridish of handling into step a, petridish is inserted in the port frame, again port is placed in the incubator, port frame outlet line is picked out incubator, be connected on the cable of main frame;
Main frame in c, the opening unit begins to carry out the program of monitoring.
The invention has the advantages that: (1) can real-time online measuring, thereby can form the culture apparatus of robotization with Other Instruments; (2) measure the influence that does not receive cell overlap, useful range can be accomplished in 4 one magnitude that cell concn changes, thereby measurement threshold is wide; (3) measure the interference do not receive dead cell and cell debris,, thereby can not reflect recording on the electric capacity because they can not form " small capacitance " of cell that kind; (4) be coated with silver electrode and prove harmless through experiment, the measuring process pair cell also can't harm itself, can continue to cultivate through the cell in the petridish of measuring.
[description of drawings]
Accompanying drawing 1 is a kind of device hardware block diagram that detects state of anchorage-dependent cell in the petridish of the present invention.
Accompanying drawing 2 is coated with the petridish of 4 parallel lines electrodes in being.
Accompanying drawing 3 is coated with the petridish of 2 parallel lines electrodes in being.
Accompanying drawing 4 is coated with the petridish of 4 circle concentric electrodes in being.
Accompanying drawing 5 is coated with the petridish of 2 circle concentric electrodes in being.
Accompanying drawing 6A is the structural representation of port frame.
Accompanying drawing 6B is the side-view of the structure of port frame.
[embodiment]
Below in conjunction with accompanying drawing a kind of embodiment that detects the devices and methods therefor of state of anchorage-dependent cell in the petridish provided by the invention is elaborated.
The Reference numeral and the integral part that relate in the accompanying drawing are as follows:
2, outer electrode 3, interior electrode
4, base 5, upper arm
6, outer electrode probe 7, interior electrode catheter
8, probe draw-in groove 9, port frame outlet line
10, mainboard 11, button keyboard
12, display module 13, cable
14, port frame 15, petridish
16, temperature detecting resistance
100, MCU unit 101, clock module
102, bridge for measuring temperature 103, magnifying glass
104, IT module 105, wave filter
106, waveform generator 107, A/D modular converter
108, battery or electric main 109, power interface
110, Voltage stabilizing module 111, push-button interface
112, display driver 113,232 chips
114,232 interfaces 115, storage unit
Embodiment 1
In be coated with 4 parallel lines electrodes petridish make
In the aseptic technique platform, by the bottom that is coated in petridish 15 shown in Figure 2, processing petridish 15 bottoms has four parallel lines silver line electrodes with conductive silver glue, and four straight line lines electrodes are left-right symmetric and distribute, and outer electrode 2 is arranged on interior electrode 3 outsides.Then petridish 15 is carried out alcohol disinfectings, nutrient solution soaks, alcohol disinfecting again, dry for standby.
In be coated with 2 parallel lines electrodes petridish make
In the aseptic technique platform, by the bottom that is coated in petridish 15 shown in Figure 3, processing petridish 15 bottoms has two parallel lines silver line electrodes with conductive silver glue, and two straight line lines electrodes are left-right symmetric and distribute.Then petridish 15 is carried out alcohol disinfectings, nutrient solution soaks, alcohol disinfecting again, dry for standby.
In be coated with 4 circle concentric electrodes petridish make
In the aseptic technique platform, by the bottom that is coated in petridish 15 shown in Figure 4, processing petridish 15 bottoms has 4 circle concentric(al) circles silver line electrodes, 4 circle loop line strip electrode and petridish 15 concentrics with conductive silver glue.Then petridish 15 is carried out alcohol disinfectings, nutrient solution soaks, alcohol disinfecting again, dry for standby.
In be coated with 2 circle concentric electrodes petridish make
In the aseptic technique platform, by the bottom that is coated in petridish 15 shown in Figure 5, processing petridish 15 bottoms has 2 circle concentric(al) circles silver line electrodes, 2 circle loop line strip electrode and petridish 15 concentrics with conductive silver glue.Then petridish 15 is carried out alcohol disinfectings, nutrient solution soaks, alcohol disinfecting again, dry for standby.
A kind of device that detects attached cell impedance in the petridish
Please with reference to Fig. 1, Fig. 2, Fig. 6 A, Fig. 6 B.
Fig. 1 is a kind of device hardware block diagram that detects state of anchorage-dependent cell in the petridish; This device is provided with main frame, petridish 15, and the main frame of described device comprises mainboard 10, button keyboard 11, display module 12, casing (not shown), cable 13 and port frame 14.Described mainboard 10 is provided with MCU unit 100, storage unit 115, clock module 101, bridge for measuring temperature 102, waveform generator 106, Voltage stabilizing module 110, push-button interface 111, display driver 112,232 chips 113.Described storage unit 115 is connected with MCU unit 100, and bridge for measuring temperature 102 is connected with MCU unit 100, and clock module 101 is connected with MCU unit 100.MCU unit 100 is connected with wave filter 105 through A/D modular converter 107, and wave filter 105 is connected with electrode in the petridish 15 through magnifying glass 103, cable 13, port frame 14.MCU unit 100 is connected with probe through waveform generator 106, IT module 104 successively.MCU unit 100 connects Voltage stabilizing module 100, and Voltage stabilizing module 100 connects battery or electric main 108 through power interface 109.MCU unit 100 connects button keyboard 11 through push-button interface 111.MCU unit 100 connects display module 12 through display driver 112.MCU unit 100 connects 232 chips, 113,232 chips 113 and connects 232 interfaces 114.Please with reference to Fig. 6 A, Fig. 6 B.Fig. 6 A and Fig. 6 B are port frame 14, and petridish 15 places in the port frame 14, the petridish that described petridish 15 selects for use embodiment 1 to make.Comprise base 4, upper arm 5, outer electrode probe 6, interior electrode catheter 7, probe draw-in groove 8, port frame outlet line 9, temperature detecting resistance 16 on the port frame 14.Described base 4 avris are provided with a support (not shown), and support is provided with a upper arm 5.Described base 4, support, upper arm 5 are processed by tetrafluoroethylene integral body.Described upper arm 5 surfaces are provided with probe draw-in groove 5, and the big termination of described interior electrode catheter 7, outer electrode probe 6 is fixed on the draw-in groove 8.The small end head that interior electrode catheter 7 small end heads are pressed close to interior electrode 3, the outer electrode probe 6 of silver-colored line is pressed close to the outer electrode 2 of silver-colored line, and (probe stationary can be according to silver-colored line electrode adjustment position and fixing in the petridish on draw-in groove.)。
Need to prove: described port frame outlet line 9 has 6; First and second root port frame outlet line 9 connects two outer electrode probes 6; Third and fourth root port frame outlet line 9 connects two interior electrode catheters 7; Five, six roots of sensation port frame outlet line 9 one ends connect temperature detecting resistance 16, and the other end connects bridge for measuring temperature 102.The effect of port frame 14 is fixing petridish 15, and port frame outlet line 9 is linked to each other with main frame.Described outer electrode probe 6, interior electrode catheter 7 can adopt the stainless steel probe, and it conducts electricity, pollution-free, and have certain corrosion resistance nature.
Current potential that probe obtains and current signal get into the MCU unit through magnifying glass, wave filter and A/D modular converter in succession; The MCU unit passes through according to prior preset program; The output frequency byte is to waveform generator (OSC); Produce the sine wave of CF and amplitude, deliver to probe segment through an IT module, current return ground connection forms the probe ground system; Isolate (device beyond the present invention's probe partly is a Device Host) with the main frame ground system, reduced the interfering factors that influences measuring result in the environment of living in of popping one's head in effectively.
Described magnifying glass 103 comprises that current potential amplification, reference electrode group and electric current amplify.
Described display module 12 is LCD display (liquid crystal display).The user uses the button input order, can obtain the data of needs, also can save the data in the storage unit 115 or through RS-232 interface data remote is arrived computingmachine.Storage unit 115 on the main frame is RAM (random access memory) and FLASH (flash memory).
The unitary major function of MCU is computing and control, with the data of measuring with in the data that computing obtains are kept at storage unit 115 or be presented on the external display module 12.These data can be specific inductivity and specific conductivity, also can be through the attached cell quantity that converts, size distribution etc.
The mainboard 10 of main frame is provided with RS232 chip 113 and RS232 interface 114, and RS232 chip 113 is connected with MCU unit 100, and RS232 interface 114 is connected with RS232 chip 113.RS232 chip 113 and RS232 interface 114 make things convenient for the present invention and extraneous data corresponding, are convenient to the application of the present invention in automatic control system.
Principle of work of the present invention is following: waveform generator 106 sends the alternating current of CF and amplitude under the unitary control of MCU; Through IT module 104, cable 13, port frame 14, the first root port frame outlet line 9 get into the outer electrode 2 of outer electrode probe 6, silver-colored line, again flow into the outer electrode 2 of interior electrode 3, the opposite side silver line of interior electrode 3, the opposite side silver line of liquid, the silver-colored line of flowing through in the petridish, through opposite side outer electrode probe 6, through the second root port frame outlet line 9, flow back into the reference resistance on the main frame mainboard.Produce voltage on the reference resistance, this voltage by current amplifier amplify, the MCU unit obtains current current signal after wave filter, AD conversion.When alternating current passes through outer electrode probe 6, silver-colored line electrode outer electrode 2 turnover liquid, set up the electric field of this frequency in the liquid, on interior electrode 3, produced voltage.Through interior electrode catheter 7, port frame outlet line 9, port frame 14, cable 13 this voltage signal is incorporated into mainboard 10.This voltage by voltage amplifier amplify, MCU unit 100 obtains current current signal after wave filter, AD conversion.Described electric potential signal (voltage signal) through the interior electrode 3 of the 3rd root port frame outlet line 9, interior electrode catheter 7, the silver-colored line of flowing through, again flow into liquid in the petridish, the interior electrode 3 through opposite side silver line, through electrode catheter in the opposite side 7, the 4th root port frame outlet line 9, port frame 14, cable 13, the current potential that this voltage signal is incorporated into mainboard 10 amplifies, MCU unit 100 obtains current electric potential signal after wave filter 105, AD conversion.
Electric current is in turnover liquid and silver-colored line electrode, and electrode surface can form polarization of electrode, produces polarization impedance Zp1 and Zp2.Frequency is low more, and polarization impedance is big more.For the higher liquid of specific conductivity, common polarization impedance was wanted obvious impedance greater than liquid when 1MHz was following.Therefore, can not think that the current potential on the outer electrode is exactly the current potential on the liquid this moment, and electrode in need using a pair, avoids polarization impedance and directly obtain the current potential on the liquid.Electric current on the interior electrode is very little, does not almost have polarization of electrode on the therefore interior electrode.When using the lower nutrient solution of specific conductivity, can in electrode and only use pair of electrodes to carry out impedance measurement.When using four electrodes, interior electrode can obtain the liquid voltage characteristic, according to Ohm's law Z=V/I, also needs the current characteristics of liquid, and this can connect with liquid through a reference resistance Rr and obtain.Voltage vector is Vx on the sample if interior electrode records, and through current phasor I, the voltage vector on the reference impedance is Vr in the sample.Because therefore polarization impedance, sample, reference resistance series connection have:
Then the sample impedance can be expressed as:
By
Can obtain:
Therefore, only need accurately to measure Vx, Vr voltage magnitude ratio and its phase differential in real time, can obtain sample impedance Z x to be measured in conjunction with demarcating good reference impedance Rr in advance.If what use is 2 electrodes, then with the voltage on this two electrode as Vx.
Because the cytolemma specific conductivity has only 1/10 of its inside and outside medium conductivity
6, therefore when applying electric field in the liquid that is comprising cell, the cell in the petridish in the liquid just becomes small one by one electric capacity.Because cytolemma is very thin, so electrical capacity is 10 in concentration
5Individual/as just can to reach the pF level during ml and be detected.Cell number is directly proportional between the electric capacity that records and electrode, through the off-line calibration before using, records the proportionlity of electric capacity and cell in advance, just can come real-time online to obtain the sum of cell through on-line measurement electric capacity.
Use proofing unit of the present invention to detect the method for state of anchorage-dependent cell in the petridish
Petridish sterilization, dry for standby that the first step: embodiment 1 makes;
Second step: by cultivating working specification; Bacterium to be inoculated is introduced a fine variety the petridish of above-mentioned processing, petridish is inserted in the port frame 14, port frame 14 places in the incubator again; Port frame outlet line 9 is carefully picked out incubator, port frame outlet line 9 is connected to the cable 13 on the main frame; The sterilization of each parts of attention port frame;
The 3rd step: open main frame, carry out the program that begins to monitor by rules.Instrument will be carried out built-in correction program at once, write down current and follow-up cultivation conditions and show.
Need to prove: the petridish that embodiment 2-4 makes also can be applied in the detection method of the present invention.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the inventive method; Can also make some improvement and replenish, these improvement and replenish and also should be regarded as protection scope of the present invention.
Claims (10)
1. device that detects state of anchorage-dependent cell in the petridish; Comprise main frame, petridish; Described main frame comprises mainboard, button keyboard, display module, casing, cable and port frame; It is characterized in that described mainboard is provided with MCU unit, storage unit, clock module, bridge for measuring temperature, waveform generator, Voltage stabilizing module, push-button interface, display driver, 232 chips
Described storage unit is connected with the MCU unit;
Bridge for measuring temperature is connected with the MCU unit;
Clock module is connected with the MCU unit;
The MCU unit is connected with wave filter through the A/D modular converter, and wave filter is connected with electrode in the petridish through magnifying glass, cable, port frame;
The MCU unit is connected with probe through waveform generator, IT module successively;
The MCU unit connects Voltage stabilizing module, and Voltage stabilizing module connects battery or electric main through power interface;
The MCU unit connects button keyboard through push-button interface;
The MCU unit connects display module through display driver;
The MCU unit connects 232 chips, and 232 chips connect 232 interfaces;
The unitary major function of said MCU is computing and control; Be kept in the storage unit or be presented on the external display module with the data of measuring with through the data that computing obtains; Said data or specific inductivity and specific conductivity, or attached cell quantity, size distribution through converting.
2. device according to claim 1 is characterized in that, scribbles electrode on the described petridish bottom interior surface, and described electrode is by two or four electrodes of painting with conductive silver glue.
3. device according to claim 2 is characterized in that, described electrode is straight line or annulus lines, and straight line lines electrode is left-right symmetric and distributes loop line strip electrode and petridish concentric.
4. device according to claim 1 is characterized in that, comprises base, upper arm, interior electrode catheter, outer electrode probe, probe draw-in groove, port frame outlet line on the described port frame.
5. device according to claim 4 is characterized in that, described base, upper arm are processed by tetrafluoroethylene integral body.
6. device according to claim 4 is characterized in that, described upper arm surface is provided with the probe draw-in groove, electrode catheter, outer electrode in described probe draw-in groove is fixing.
7. device according to claim 1 is characterized in that, described MCU unit is a dsp chip, and waveform generator is a Direct Digital Frequency Synthesizers.
8. device according to claim 7 is characterized in that described dsp chip is selected the TMS32C2812 chip for use, and Direct Digital Frequency Synthesizers is selected the AD9834 chip for use.
9. device according to claim 1 is characterized in that, described storage unit is RAM and FLASH.
10. method of utilizing the described device of claim 1 to detect state of anchorage-dependent cell in the petridish comprises following key step:
A, conductive silver glue is coated in the petridish internal surface by electrode desired shape and position, again with petridish sterilization, nutrient solution soak, sterilization, dry for standby again;
B, bacterium to be inoculated is introduced a fine variety the petridish of handling into step a, petridish is inserted in the port frame, again port is placed in the incubator, port frame outlet line is picked out incubator, be connected on the cable of main frame;
Main frame in c, the opening unit begins to carry out the program of monitoring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200810041988 CN101654651B (en) | 2008-08-22 | 2008-08-22 | Device and method for detecting state of anchorage-dependent cell in culture dish |
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| CN 200810041988 CN101654651B (en) | 2008-08-22 | 2008-08-22 | Device and method for detecting state of anchorage-dependent cell in culture dish |
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| CN101654651A CN101654651A (en) | 2010-02-24 |
| CN101654651B true CN101654651B (en) | 2012-07-04 |
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| CN105555944B (en) * | 2013-10-11 | 2017-03-15 | 松下健康医疗控股株式会社 | Culture apparatus |
| CN114544468A (en) * | 2022-02-25 | 2022-05-27 | 成都柏奥特克生物科技股份有限公司 | Capacitance method cell counting method for fixed bed bioreactor |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000125846A (en) * | 1998-10-27 | 2000-05-09 | Matsushita Electric Ind Co Ltd | Device for measuring number of microorganism and method therefor |
| CN1504578A (en) * | 2002-11-29 | 2004-06-16 | 公准精密工业股份有限公司 | Method and device for detecting biological cell |
| CN1834220A (en) * | 2005-07-07 | 2006-09-20 | 重庆大学 | Microstress applicator for in vitro cell |
| CN101000306A (en) * | 2006-01-09 | 2007-07-18 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyser |
-
2008
- 2008-08-22 CN CN 200810041988 patent/CN101654651B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000125846A (en) * | 1998-10-27 | 2000-05-09 | Matsushita Electric Ind Co Ltd | Device for measuring number of microorganism and method therefor |
| CN1504578A (en) * | 2002-11-29 | 2004-06-16 | 公准精密工业股份有限公司 | Method and device for detecting biological cell |
| CN1834220A (en) * | 2005-07-07 | 2006-09-20 | 重庆大学 | Microstress applicator for in vitro cell |
| CN101000306A (en) * | 2006-01-09 | 2007-07-18 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyser |
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