CN1267116C - Medicine for treating gingivitis and periodontitis and preparation method thereof - Google Patents

Abstract

The present invention provides a medicament for treating gingivitis and parodontitis and a preparation method thereof. The medicament is oral administration medicine prepared from honeysuckle, scutellaria, patchouli, peony bark, dahurian angelica root and herba, preferably troche, capsule, granular agents and oral liquid. Pharmacodynamic experimental research indicates that the medicament has good functions on treating gingivitis and parodontitis, and high use value in clinic.

Description

A kind of medicine for the treatment of gingivitis and periodontitis and preparation method thereof

Technical field

The invention belongs to the field of Chinese medicines, the invention provides a kind of medicine for the treatment of gingivitis and periodontitis and preparation method thereof.

Background technology

At present the kind of buccal tablet all concentrates on the lozenge aspect of treatment laryngopharyngeal diseases, and the red swelling of gingiva pain, the halitosis that occupy the oral disease first place be nobody shows any interest in.The reason that causes red swelling of gingiva pain, halitosis is a lot, and is wherein common with gingivitis, periodontitis.This disease can make gingiva congestion and edema, pyorrhea, hemorrhage, halitosis, odontoseisis, displacement, final loss of tooth.So sick the damage occur the ability of chewing is reduced, influence digestive function, and be an impediment to attractive in appearance and doings.The further sick damage of periodontal tissue can influence tissue of tooth, and ascending pulpitis and periapical periodontitis take place.Inflammation spreads, and often forms the fascial space infection of periodontal abscess and jaw face.Antibacterial and toxin in the periodontal pocket enter blood, can cause bacteremia and toxemia, cause bacterial endocarditis, myocarditis, nephritis, arthritis and oculopathy etc.Along with modern society's civilization progress, people will more and more pay attention to the disease of this influence individual sight, obstruction doings.

At present the western medical treatment primary disease does not also have desirable medicine, the buccal tablet patent medicine preparation easy to use that particularly is positioned at halitosis, red swelling of gingiva pain lack especially as.Do not treat halitosis, red swelling of gingiva pain, gingival marginal atrophy in the new varieties of approved listing especially so that influence the Chinese patent medicine of beautiful teeth.Therefore, the medicine of inventing a kind of effective treatment gingivitis and periodontitis is being badly in need of at present clinically very much.

Summary of the invention

The object of the present invention is to provide a kind of medicine for the treatment of gingivitis and periodontitis and preparation method thereof.

The medicine of treatment gingivitis of the present invention and periodontitis is that the feedstock production by following weight parts forms:

Flos Lonicerae 500-800 Radix Scutellariae 400-600

Herba Pogostemonis 300-550 Cortex Moutan 150-350

Radix Angelicae Dahuricae 200-400 Herba Moslae 200-400

The weight ratio of above-mentioned raw materials is preferred:

Flos Lonicerae 500 Radix Scutellariaes 450 Herba Pogostemonis 320

Cortex Moutan 200 Radixs Angelicae Dahuricae 250 Herba Moslaes 250

The weight ratio of above-mentioned raw materials is also preferred:

Flos Lonicerae 625 Radix Scutellariaes 500 Herba Pogostemonis 438

Cortex Moutan 250 Radixs Angelicae Dahuricae 312 Herba Moslaes 312

The weight ratio of above-mentioned raw materials is also preferred:

Flos Lonicerae 750 Radix Scutellariaes 580 Herba Pogostemonis 500

Cortex Moutan 350 Radixs Angelicae Dahuricae 380 Herba Moslaes 380

The weight ratio of above-mentioned raw materials is also preferred:

Flos Lonicerae 650 Radix Scutellariaes 420 Herba Pogostemonis 300

Cortex Moutan 160 Radixs Angelicae Dahuricae 300 Herba Moslaes 300

Medicine of the present invention can be the oral Pharmaceutical dosage forms of any routine.Preferred buccal tablet, capsule, granule and oral liquid.

Buccal tablet of the present invention can be prepared from by the following method:

1) extracting honeysuckle, Herba Pogostemonis, Cortex Moutan (coarse powder), the Radix Angelicae Dahuricae (coarse powder), Herba Moslae add 6-15 times of water gaging, soak 0.5-2 hour, heating and refluxing extraction 3-8 hour, collect volatile oil; Medicinal liquid filters, and filtrate and medicinal residues are standby; In volatile oil, add equivalent ethanol (concentration range 50%-100%) dissolving, get 2-8, add 1-5 and doubly mix thoroughly to the water of cyclodextrin amount doubly to the cyclodextrin of volatilization oil mass, slowly drip volatile oil ethanol liquid, grind drying, cross the 40-120 mesh sieve, it is standby to get cyclodextrin clathrate;

2) get Radix Scutellariae, use water extraction, add boiling water for the first time and decoct, for the second time with above five kinds of Chinese medicine distillating extracting oil after medicinal residues merge, decoct with water again, filter, filtrate and boiling water decoction liquor merging for the first time, be concentrated into relative density 0.8-1.5 (60 ℃ of surveys), centrifugal, supernatant continues to be evaporated to the thick paste of relative density 1.0-2.0 (60 ℃ of surveys), vacuum drying, pulverize, get extract powder;

3) get extract powder, add cane sugar powder, acesulfame potassium, citric acid, use the 50%-100% alcohol granulation, cross the 8-20 order, 50～60 ℃ of dryings, with 12-20 mesh sieve granulate, adding cyclodextrin clathrate, hardship are covered essence, apple essence and an amount of magnesium stearate, mixing, tabletting, the bag film-coat, packing promptly gets buccal tablet.

The preparation method of capsule of the present invention, granule and oral liquid is: step is with above-mentioned 1), 2) step, the 3rd) step undertaken by the method for preparing capsule, granule and oral liquid of this area routine, make capsule of the present invention, granule or oral liquid.

Among the present invention, cyclodextrin can be α-, β-, gamma-cyclodextrin or other cyclodextrin derivative.

Embodiment 1

Flos Lonicerae 500 Radix Scutellariaes 450 Herba Pogostemonis 320

Cortex Moutan 200 Radixs Angelicae Dahuricae 250 Herba Moslaes 250

Preparation method is as follows:

1) extracting honeysuckle, Herba Pogostemonis, Cortex Moutan (coarse powder), the Radix Angelicae Dahuricae (coarse powder), Herba Moslae add 10 times of water gagings, soak 1 hour, and heating and refluxing extraction 6 hours is received volatile oil; Medicinal liquid filters, and filtrate and medicinal residues are standby; Volatile oil adds the equivalent dissolve with ethanol, gets 5 times to volatilization oil mass β-CD, adds 3 times of water to β-CD amount and mixes thoroughly, slowly drips volatile oil ethanol liquid, grinds 3 hours, and 40 ℃ of dryings are crossed 80 mesh sieves, and it is standby to get beta-CD inclusion.

2) get Radix Scutellariae, use water extraction, go into 10 times of amount boiling water for the first time and decocted 1.5 hours, for the second time with distillating extracting oil after medicinal residues merge, add 8 times of water gagings and decocted 1 hour, filter, filtrate and aforementioned medicinal liquid merge, be concentrated into relative density 1.08 (60 ℃ of surveys), centrifugal, supernatant continues to be evaporated to the thick paste of relative density about 1.30 (60 ℃ of surveys), vacuum drying, pulverize, get extract powder.

3) get extract powder, add cane sugar powder, acesulfame potassium, citric acid, use 95% alcohol granulation, cross 14 orders, 50～60 ℃ of dryings, with 16 mesh sieve granulate, add beta-CD inclusion, hardship cover essence,, apple essence and an amount of magnesium stearate, mixing, tabletting, the bag film-coat, packing promptly gets buccal tablet.

Embodiment 2

Flos Lonicerae 625 Radix Scutellariaes 500 Herba Pogostemonis 438

Cortex Moutan 250 Radixs Angelicae Dahuricae 312 Herba Moslaes 312

Preparation method is with embodiment 1.

Embodiment 3

Flos Lonicerae 750 Radix Scutellariaes 580 Herba Pogostemonis 500

Cortex Moutan 350 Radixs Angelicae Dahuricae 380 Herba Moslaes 380

Preparation method is:

Step 1) and 2) with embodiment 1, the 3) go on foot and carry out according to the capsular method of the preparation of this area routine, make capsule of the present invention.

Embodiment 4

Flos Lonicerae 650 Radix Scutellariaes 420 Herba Pogostemonis 300

Cortex Moutan 160 Radixs Angelicae Dahuricae 300 Herba Moslaes 300

Preparation method is:

Step 1) and 2) with embodiment 1, the 3) go on foot and carry out according to the method for preparing granule of this area routine, make granule of the present invention.

Embodiment 5

Flos Lonicerae 650 Radix Scutellariaes 420 Herba Pogostemonis 300

Cortex Moutan 160 Radixs Angelicae Dahuricae 300 Herba Moslaes 300

Preparation method is:

Step 1) and 2) with embodiment 1, the 3) go on foot and carry out according to the method for preparing oral liquid of this area routine, make oral liquid of the present invention.

One, pharmacodynamics data

Pharmacodynamic experiment studies show that medicine of the present invention has the effect of good treatment gingivitis and periodontitis.Mainly show: (1) can reduce gingivitis model Cavia porcellus incisor and organize the soak antibacterial to detect positive rate, improve model Cavia porcellus red swelling of gingiva, quality is soft, the performance indicators that gingival sulcus is deepened, change model Cavia porcellus gingival epithelium ulcer, the local inflammation necrosis, collagen is loosened, and the pathology of serious gingiva inflammation such as mucosa holostrome inflammation change.(2) can control the periodontitis rat model and fur gloss occur, cold extremities is unable, inappetence, even performance such as death, and the rat body weight that has stoped this periodontitis to cause substantially descends.(3) soft to the erosion of rat model maxillary molar surrounding tissue, necrosis, tooth blackout, quality, and upper jaw gingival epithelium ulcer, the local inflammation necrosis, collagenolysis, mucosal inflammation, alveolar bone, spongy bone occur that the small amount of bone girder is sparse, pathological change such as attenuate all has better improvement, show that it has therapeutical effect preferably to Cavia porcellus gingivitis, rat periodontitis.(4) can obviously reduce Hemolytic streptococcus type strain and clinical strain and infect the back mortality of mice; Reduce the mortality rate of hemophilus influenza type strain and clinical strain infecting mouse, have certain vivo bacteria corrosion action.(5) can suppress the mice caused by dimethylbenzene xylene auricle edema; Suppress Ovum Gallus domesticus album and cause rat paw edema; Reduce sodium carboxymethyl cellulose and cause the rat abdominal cavity leukoplania, have certain antiinflammatory action.The mouse writhing reaction that (6) can suppress to cause by acetic acid; Improve the temperature capacity of mice, have analgesic activity preferably.(7) can shorten blood clotting time of mice; Shorten the plasma in rabbit recalcification time, have certain anastalsis.(8) can promote the phagocytic activity of mice reticuloendothelial system; Promote the hemolytic antibody of mice to generate, have the effect that improves immunologic function.

1. experiment material

1.1 medicine and reagent

Hemolytic streptococcus type strain and clinical separation strain, hemophilus influenza type strain and clinical separation strain, clinical periodontal pocket anaerobe is provided by microorganism teaching and research room of the Hunan Province college of traditional Chinese medicine.Clinical periodontal pocket anaerobe isolated culture method: from clinical patients with periodontitis periodontal pocket, obtain bacterial plaque, cultivate, obtain the total anaerobic culture liquid of dental plaque by anaerobism with syringe.

2. method and result

(statistical method: measurement data represents that with X ± S the neat person of variance is with variance analysis and t check analysis in groups; The heterogeneity of variance person is with the rank test analysis.Enumeration data is with X ²Check analysis.Ranked data are with the Ridit check analysis.)

2.1. influence to Cavia porcellus gingivitis model

2.1.1 method

Modeling: fasten knot Cavia porcellus lower jaw incisor neck portion with just abnormal steel wire, the anaerobic culture liquid of clinical patients periodontal pocket bacterial plaque is inoculated into fastening knot Cavia porcellus gingival sulcus, caused the acute gingivitis model in continuous 5 days with probe.

Get 60 of Cavia porcelluss; male and female half and half; body weight 425.6 ± 16.5g; be divided into 6 groups at random by body weight; be respectively blank group (ig distilled water 10ml/kg), model control group (ig distilled water 10ml/kg), Bezoar pill for clearing away the stomach-heat group (ig Bezoar pill for clearing away the stomach-heat 3.8g/kg; be 2 times of clinical equivalent dosage), the health gum basic, normal, high dosage group of buccal tablet (clean the teeth buccal tablet 2.3,4.6,9.2g crude drug/kg of ig health gum respectively is equivalent to 1,2,4 times of clinical equivalent dosage respectively) that cleans the teeth.Except that the blank group, all the other animal modelings, all Cavia porcelluss are pressed table 1 dosed administration in the 1st day every Mus of modeling, and every day 1 time, continuous 5 days, medication is: per os splashed into, and swallows naturally.Observation index: A, gingiva inflammation are observed.B, get incisor and the surrounding tissue soak carries out bacteriology checking.C, get the conventional paraffin wax section of incisor and surrounding tissue, HE dyeing is observed the gingiva pathological change: 1. gingiva tissue structure normal (-); 2. the gingival epithelium covering is complete, and a small amount of vasodilation of lamina propria is dispersed in a small amount of inflammatory cell infiltration (+); 3. gingival epithelium is not seen erosion, lamina propria inflammatory cell focal infiltration, collagen fiber swelling, vasodilation, congested (++); 4. gingival epithelium ulcer, local a large amount of inflammatory necrosis tissues, collagenolysis, inflammation is involved mucosa holostrome (+++).

The table 1 health gum buccal tablet that cleans the teeth is organized the bacteriological influence of soak to gingivitis model Cavia porcellus incisor

Group	Number of animals (only)	Dosage (g/kg)	Periodontal bacteria is checked
					Number positive	Positive rate %
			The blank group model matched group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth is high in low	10 10 10 10 10 10			DW DW 3.8 2.3 4.6 9.2	1 9 6 7 6 5	10 ** 90 60 70 60 50 *

Through X ²Check is compared with model control group, ^*P＜0.05, ^*P＜0.01

Table 2 health gum cleans the teeth buccal tablet to the histopathologic influence of gingivitis model Cavia porcellus incisor

Group	Number of animals (only)	The result of histopathologic examination (only)
						-	+	++	+++
		The blank group model matched group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth is high in low	10 10 10 10 10 10	10 0 0 0 0 1	0 1 4 5 5 5					0 3 5 3 4 4	0 6 1 * 2 1 * 0 **

Through the Ridit check, compare with model control group, ^*P＜0.05, ^*P＜0.01

2.1.2 result

Characterize observed result: model control group Cavia porcellus red swelling of gingiva, edema, quality is soft, and gingival sulcus is deepened, and gently leans out blood.The health gum cleans the teeth, and show is swollen slightly for the middle and high dosage group of buccal tablet Cavia porcellus gingiva, edema.

By table 1 as seen, with model control group relatively, the health gum buccal tablet high dose group Cavia porcellus incisor that cleans the teeth is organized the soak bacteriology to detect positive rate significantly to reduce.

By table 2 as seen, the most gingival epithelium ulcer of model control group Cavia porcellus, local a large amount of inflammatory necrosis tissues, collagenolysis, inflammation is involved the mucosa holostrome, shows serious gingiva inflammation; Buccal tablet low dose group animal gingival epithelium is not seen erosion though the health gum cleans the teeth, lamina propria inflammatory cell focal infiltration, and collagen fiber swelling, vasodilation, hyperemia is during majority shows as, intense gingivitis disease; The most gingival epitheliums of middle and high dosage treated animal cover complete, a small amount of vasodilation of lamina propria, and tissue edema is not obvious, only is dispersed in a small amount of inflammatory cell infiltration, and collagen fiber swelling is not obvious, shows as light, moderate gingiva inflammation.Through Ridit check, with model control group relatively, the clean the teeth middle and high dosage group of buccal tablet Cavia porcellus incisor and surrounding tissue pathological change of health gum is remarkable, shows that the health gum buccal tablet that cleans the teeth has therapeutical effect preferably to the Cavia porcellus acute gingivitis.

2.3. bacteriostasis research

2.3.1 influence to Hemolytic streptococcus type strain and the death of clinical strain infecting mouse

2.3.1.1 method

Get 120 of ICR system cleaning level mices; male and female half and half; body weight 21.3 ± 1.5g; be divided into 6 groups at random; be respectively model control group (ig distilled water 20ml/kg), amoxicillin group (0.4g/kg; be 2 times of clinical equivalent dosage), Bezoar pill for clearing away the stomach-heat group (ig Bezoar pill for clearing away the stomach-heat 6.2g ball/kg; be 2 times of clinical equivalent dosage), the health gum basic, normal, high dosage group of buccal tablet (clean the teeth buccal tablet 3.8,7.6,15.2g crude drug/kg of ig health gum respectively is equivalent to 1,2,4 times of clinical equivalent dosage respectively) that cleans the teeth.Press table 5 dosed administration 6 times, medication is: irritate earlier stomach, stay about 0.1ml allow the mice buccal at last.Administration every day is 1 time before bacterial injection, and totally 3 times, 30min after the 3rd administration, (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 1 * 10 to every Mus lumbar injection 0.5ml second kind Hemolytic streptococcus liquid (type strain) ⁹Individual/ml), administration 3 times is continued in 1h and the 2nd, 3 day behind the injection Hemolytic streptococcus, and the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.

Other gets 120 of ICR system cleaning level mices, male and female half and half, body weight 22.8 ± 2.0g, administration as stated above, and (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 5 * 10 to inject 0.5ml second kind Hemolytic streptococcus liquid (clinical separation strain) ⁸Individual/ml), the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.The results are shown in Table 5.

The influence of buccal tablet that clean the teeth of table 5 health gum to the hemolytic streptococcal infection dead mouse

Group	Number of animals (only)	Dosage (g/kg)	Type strain		Clinical separation strain
							Death toll (only)	Mortality rate (%)	Death toll (only)	Mortality rate (%)
			The group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth in model control group amoxicillin is high in low	20 20 20 20 20 20	DW 0.4 6.2 3.8 7.6 15.2	18 0 12 13 11 11					90 0 ** 60 * 65 55 * 55 *	19 1 13 14 12 11	95 5 ** 65 * 70 * 60 * 55 **

Through X ²Relatively, compare with model control group, ^*P＜0.05, ^*P＜0.01

2.3.1.2 result

Table 5 as seen, compare with model control group, the health gum basic, normal, high dosage group of the buccal tablet mortality of mice that cleans the teeth has significant difference, its mortality rate obviously reduces, show that the health gum buccal tablet that cleans the teeth can obviously reduce Hemolytic streptococcus type strain and clinical separation strain and infects the back mortality of mice, has certain antibacterial action.The positive control drug Amoxicillin Capsules has good vivo bacteria corrosion action.

2.3.2 influence to hemophilus influenza type strain and the death of clinical strain infecting mouse

2.3.2.1 method

Get 120 of ICR system cleaning level mices; male and female half and half; body weight 21.6 ± 2.6g; be divided into 6 groups at random; be respectively model control group (ig distilled water 20ml/kg), amoxicillin group (0.4g/kg; be 2 times of clinical equivalent dosage), Bezoar pill for clearing away the stomach-heat group (ig Bezoar pill for clearing away the stomach-heat 6.2g ball/kg; be 2 times of clinical equivalent dosage), the health gum basic, normal, high dosage group of buccal tablet (clean the teeth buccal tablet 3.8,7.6,15.2g crude drug/kg of ig health gum respectively is equivalent to 1,2,4 times of clinical equivalent dosage respectively) that cleans the teeth.Press table 6 dosed administration 6 times, medication is: irritate earlier stomach, stay about 0.1ml allow the mice buccal at last.Administration every day is 1 time before bacterial injection, and totally 3 times, 30min after the 3rd administration, (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 1.5 * 10 to every Mus lumbar injection 0.5ml hemophilus influenza liquid (type strain) ⁹Individual/ml), administration 3 times is continued in 1h and the 2nd, 3 day behind the injected current haemophilus influenza, and the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.

Other gets 120 of ICR system cleaning level mices, male and female half and half, and body weight 22.1 ± 2.4g, administration as stated above, and (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 1 * 10 to inject 0.5ml hemophilus influenza liquid (clinical separation strain) ⁹Individual/ml), dead mouse situation in the observed and recorded 4 days is carried out statistical with mortality rate.The results are shown in Table 6.

The influence of buccal tablet that clean the teeth of table 6 health gum to the death of hemophilus influenza infecting mouse

Group	Number of animals (only)	Dosage (g/kg)	Type strain		Clinical separation strain
							Death toll (only)	Mortality rate (%)	Death toll (only)	Mortality rate (%)
			The group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth in model control group amoxicillin is high in low	20 20 20 20 20 20	DW 0.4 6.2 3.8 7.6 15.2	19 0 14 15 13 12					95 0 ** 70 * 75 65 * 60 **	19 0 15 14 14 13	95 0 ** 75 70 * 70 * 65 *

Through X ²Relatively, compare with model control group, ^*P＜0.05, ^*P＜0.01

2.3.2.2 result

Table 6 as seen, with model control group relatively, the health gum basic, normal, high dosage group of the buccal tablet mortality of mice that cleans the teeth has significant difference, its mortality rate obviously reduces, show that the health gum buccal tablet that cleans the teeth can obviously reduce hemophilus influenza and infects the back mortality of mice, has certain antibacterial action.The positive control drug Amoxicillin Capsules has fabulous vivo bacteria corrosion action.

Two, updates

1. experiment material

1.1 medicine and reagent

The health gum buccal tablet that cleans the teeth, clinical plan consumption is 29.3g crude drug/day, adopts its extractum during experiment, and concentration is 4.4g crude drug/g, and Drug Manufacturing Room provide by the Hunan Inst of Traditional Chinese Medicine, lot number: 20030528.

Positive drug (one), Bezoar pill for clearing away the stomach-heat, specification: every ball weighs 6 grams; Consumption: oral, 4 ball/days.Hebei province Cang Zhou Tang Sheng pharmaceutcal corporation, Ltd produces, lot number: 20010524.

Positive drug (two), Metronidazole Tablet, specification 0.2g/ sheet, oral 2-3 sheet/time, 3 times/day, Central China, Hubei pharmaceutical Co. Ltd produces, lot number: 021210.

Porphyromonas gingivalis (ATCC33277), middle general Salmonella (ATCC25261) is provided by BJ Stomatological Hospital's institute.Porphyromonas gingivalis, middle general Salmonella clinical separation strain are separated by clinical patients with periodontitis periodontal pocket, and the antibacterial increment is cultivated and provided by microorganism teaching and research room of Hunan College of Traditional Chinese Medicine.

1.2 animal

NIH one-level mice, male and female half and half, body weight 18-22g is provided by Hunan College of Traditional Chinese Medicine's Experimental Animal Center, the quality certification number: 20-001 number.The laboratory animal environmental facility quality certification number: No. 027.

2. method and result

(statistical method: measurement data represents that with X ± S the neat person of variance is with variance analysis and t check analysis in groups; The heterogeneity of variance person is with the rank test analysis.Enumeration data is with X ²Check analysis.Ranked data are with the Ridit check analysis.)

2.1. bacteriostasis research

2.1.1 influence to porphyromonas gingivalis type strain and the death of clinical strain infecting mouse

2.1.1.1 method ⁽⁴⁾

Get 120 of ICR system cleaning level mices; male and female half and half; body weight 19.6 ± 3.8g; be divided into 6 groups at random; be respectively model control group (ig distilled water 20ml/kg), metronidazole group (0.4g/kg; be 2 times of clinical equivalent dosage), Bezoar pill for clearing away the stomach-heat group (ig Bezoar pill for clearing away the stomach-heat 6.2g ball/kg; be 2 times of clinical equivalent dosage), the health gum basic, normal, high dosage group of buccal tablet (clean the teeth buccal tablet 3.8,7.6,15.2g crude drug/kg of ig health gum respectively is equivalent to 1,2,4 times of clinical equivalent dosage respectively) that cleans the teeth.Press table 3 dosed administration 6 times, medication is: irritate earlier stomach, stay about 0.1ml allow the mice buccal at last.Administration every day is 1 time before bacterial injection, and totally 3 times, 30min after the 3rd administration, (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 1.2 * 10 to every Mus lumbar injection 0.5ml second kind porphyromonas gingivalis liquid (type strain) ¹⁰Individual/ml), administration 3 times is continued in 1h and the 2nd, 3 day behind the injection porphyromonas gingivalis, and the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.

Other gets 120 of ICR system cleaning level mices, male and female half and half, body weight 21.4 ± 4.4g, administration as stated above, and (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 6 * 10 to inject 0.5ml porphyromonas gingivalis liquid (clinical separation strain) ⁸Individual/ml), the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.The results are shown in Table 3.

The influence of buccal tablet that clean the teeth of table 3 health gum to the death of porphyromonas gingivalis infecting mouse

Group	Number of animals (only)	Dosage (g/kg)	Type strain		Clinical separation strain
							Death toll (only)	Mortality rate (%)	Death toll (only)	Mortality rate (%)
			The model control group metronidazole group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth is high in low	20 20 20 20 20 20	DW 0.4 6.2 3.8 7.6 15.2	19 2 14 14 14 12					95 10 ** 70 * 70 * 70 * 60 **	19 3 15 16 14 13	95 15 ** 75 80 70 * 65 *

Through X ²Relatively, compare with model control group, ^*P＜0.05, ^*P＜0.01

2.1.1.2 result

Table 3 as seen, compare with model control group, the health gum basic, normal, high dosage group of the buccal tablet mortality of mice that cleans the teeth has significant difference, its mortality rate obviously reduces, show that the health gum buccal tablet that cleans the teeth can obviously reduce mortality of mice behind porphyromonas gingivalis type strain and the clinical strain intraperitoneal injection of mice, has certain antibacterial action.Positive control drug metronidazole capsule has vivo bacteria corrosion action preferably.

2.2.2 influence to general Salmonella type strain in centre and the death of clinical strain infecting mouse

2.2.2.1 method

Get 120 of ICR system cleaning level mices; male and female half and half; body weight 20.8 ± 3.6g; be divided into 6 groups at random; be respectively model control group (ig distilled water 20ml/kg), metronidazole group (0.4g/kg; be 2 times of clinical equivalent dosage), Bezoar pill for clearing away the stomach-heat group (ig Bezoar pill for clearing away the stomach-heat 6.2g ball/kg; be 2 times of clinical equivalent dosage), the health gum basic, normal, high dosage group of buccal tablet (clean the teeth buccal tablet 3.8,7.6,15.2g crude drug/kg of ig health gum respectively is equivalent to 1,2,4 times of clinical equivalent dosage respectively) that cleans the teeth.Press table 4 dosed administration 6 times, medication is: irritate earlier stomach, stay about 0.1ml allow the mice buccal at last.Administration every day is 1 time before bacterial injection, and totally 3 times, 30min after the 3rd administration, (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 1.5 * 10 to general Salmonella liquid (type strain) in the middle of every Mus lumbar injection 0.5ml ¹⁰Individual/ml), the death condition of mice is carried out statistical with mortality rate in the 1h and continued administration 3 times on the the 2nd, 3 day behind the general Salmonella in the middle of the injection, observed and recorded 4 days.

Other gets 120 of ICR system cleaning level mices, male and female half and half, and body weight 22.4 ± 5.0g, administration as stated above, and (draw this bacterium infecting mouse by prerun, the concentration that 90% mortality rate occurs is about 9 * 10 to inject the middle general Salmonella liquid (clinical separation strain) of 0.5ml ⁸Individual/ml), the death condition of mice is carried out statistical with mortality rate in the observed and recorded 4 days.The results are shown in Table 4.

The influence of buccal tablet that clean the teeth of table 4 health gum to centre general Salmonella infecting mouse death

Group	Number of animals (only)	Dosage (g/kg)	Type strain		Clinical separation strain
							Death toll (only)	Mortality rate (%)	Death toll (only)	Mortality rate (%)
			The model control group metronidazole group Bezoar pill for clearing away the stomach-heat group health gum buccal tablet that cleans the teeth is high in low	20 20 20 20 20 20	DW 0.4 6.2 3.8 7.6 15.2	19 3 15 15 14 13					95 15 ** 75 75 70 * 65 *	19 5 15 14 14 14	95 25 ** 75 70 * 70 * 70 *

Through X ²Relatively, compare with model control group, ^*P＜0.05, ^*P＜0.01

2.2.2.2 result

Table 4 as seen, with model control group relatively, the health gum basic, normal, high dosage group of the buccal tablet mortality of mice that cleans the teeth has significant difference, its mortality rate obviously reduces, show the health gum clean the teeth buccal tablet in the middle of obviously reducing general Salmonella infect the back mortality of mice, have certain antibacterial action.Positive control drug metronidazole capsule has vivo bacteria corrosion action preferably.

3. discuss

Porphyromonas gingivalis and middle general Salmonella are the Gram-negative anaerobic bacillus(cillus anaerobicus), study morely in recent years, are considered to main bacteria infection such as periodontitis.From above-mentioned result of study as seen, the health gum buccal tablet that cleans the teeth has certain inhibitory action to the two.

Claims (8)

1, a kind of medicine for the treatment of gingivitis and periodontitis is characterized in that making raw materials of effective components and consists of:

Flos Lonicerae 500-800 Radix Scutellariae 400-600

Herba Pogostemonis 300-550 Cortex Moutan 150-350

Radix Angelicae Dahuricae 200-400 Herba Moslae 200-400.

2, medicine as claimed in claim 1 is characterized in that the weight ratio of raw material is:

Flos Lonicerae 500 Radix Scutellariaes 450 Herba Pogostemonis 320

Cortex Moutan 200 Radixs Angelicae Dahuricae 250 Herba Moslaes 250.

3, medicine as claimed in claim 1 is characterized in that the weight ratio of raw material is:

Flos Lonicerae 625 Radix Scutellariaes 500 Herba Pogostemonis 438

Cortex Moutan 250 Radixs Angelicae Dahuricae 312 Herba Moslaes 312.

4, medicine as claimed in claim 1 is characterized in that the weight ratio of raw material is:

Flos Lonicerae 750 Radix Scutellariaes 580 Herba Pogostemonis 500

Cortex Moutan 350 Radixs Angelicae Dahuricae 380 Herba Moslaes 380.

5, medicine as claimed in claim 1 is characterized in that the weight ratio of raw material is:

Flos Lonicerae 650 Radix Scutellariaes 420 Herba Pogostemonis 300

Cortex Moutan 160 Radixs Angelicae Dahuricae 300 Herba Moslaes 300.

6, medicine as claimed in claim 1 is characterized in that this medicine is buccal tablet, granule, capsule or oral liquid.

7, the preparation method of the described medicine of claim 6 is characterized in that preparing buccal tablet and may further comprise the steps:

1) extracting honeysuckle, Herba Pogostemonis, Cortex Moutan, the Radix Angelicae Dahuricae, Herba Moslae add 6-15 times of water gaging, soak 0.5-2 hour, heating and refluxing extraction 3-8 hour, collect volatile oil; Medicinal liquid filters, and filtrate and medicinal residues are standby; Add equivalent 50%-100% dissolve with ethanol in volatile oil, get 2-8 doubly to the cyclodextrin of the oil mass of volatilizing, add 1-5 and doubly mix thoroughly to the water of cyclodextrin amount, slowly drip volatile oil ethanol liquid, grind, drying is crossed the 40-120 mesh sieve, and it is standby to get cyclodextrin clathrate;

2) get Radix Scutellariae, use water extraction, add boiling water for the first time and decoct, for the second time with above five kinds of Chinese medicine distillating extracting oil after medicinal residues merge, decoct with water again, filter, filtrate and boiling water decoction liquor merging for the first time, be concentrated into relative density 0.8-1.5, centrifugal, supernatant continues to be evaporated to the thick paste of relative density 1.0-2.0, vacuum drying, pulverize, get extract powder;

3) get extract powder, add cane sugar powder, acesulfame potassium, citric acid, use the 50%-100% alcohol granulation, cross the 8-20 order, 50～60 ℃ of dryings, with 12-20 mesh sieve granulate, adding cyclodextrin clathrate, hardship are covered essence, apple essence and an amount of magnesium stearate, mixing, tabletting, the bag film-coat, packing promptly gets buccal tablet.

8, preparation method according to claim 7 is characterized in that cyclodextrin wherein is alpha-cyclodextrin, beta-schardinger dextrin-, gamma-cyclodextrin or other cyclodextrin derivative.