CN1266897A - Process for fermenting lucid ganoderma in 18-ton fermentor - Google Patents
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Abstract
A process for fermenting lucid ganoderma in 18-ton fermentor is an improvement on the formulation of culture medium, technological steps and fermenting parameters for dissolving the problems of poor oxygen dissolving power, easy infection of bacteria and no coordination of parameters. Its advantages are large-scale fermentation, high yield of dried lucid ganoderma hyppha powder (up to 1-1.5%) and high content of ganoderma polysaccharides in its dried hypha powder (up to 7-9 g/100g).
Description
The present invention relates to a kind of lucid ganoderma in 18-ton fermentor deep liquid fermentation process.
Glossy ganoderma is a kind of fungi with very high economic worth, be exactly since ancient times nourish, keep fit, diseases prevention, the treasure of curing the disease.Modern medicine proves that the drug effect of glossy ganoderma is very extensive, and diseases such as cancer, heart trouble, cerebral thrombosis, asthma, chronic hepatitis, ephritis, stomach trouble, neurasthenia are all had very significant curative effect, and almost non-toxic side effect.Glossy ganoderma also contains a large amount of trace elements simultaneously, can promote human body to take the photograph the oxygen ability, makes blood circulation unimpeded, prevents the formation of color spot, wrinkle, thrombus, and can promote cell metabolism, delays the aging of body, is a kind of natural cosmetology and health good merchantable brand.Exactly because glossy ganoderma has the medical care effect of mystery like this, at present in the international market, especially started one glossy ganoderma exploitation heat in countries and regions such as Japan in Asia, Korea S, China, Taiwan, Hong Kong, South East Asia.Ganoderma lucidum prod no longer is confined to medicine, but enters industries such as food, beverage, makeup, articles for washing more.Along with the raising of living standards of the people and quality of life, in the society to the demand of glossy ganoderma with increasing.But the wild resource of glossy ganoderma seldom, is difficult to satisfy the demand, so required glossy ganoderma mainly is to obtain by artificial culture and mycelial liquid submerged fermentation on the present industrial production.Because having, artificial culture takes up an area of greatly, cycle is long, be subjected to many shortcomings such as the big thereby difficult quality guarantee of weather effect is big, cost is high, plant in the different place of production of different batches glossy ganoderma mass discrepancy, the glossy ganoderma of especially serious is artificial growth is sprayed sometimes or pollutes some agricultural chemicals and sterilant, can cause serious harm to human body when taking.And the mycelium liquid submerged fermentation have occupation of land less, cycle weak point, weatherproof, cost is low, quality good, the ganoderma lucidum powder mass discrepancy of different batches is little, do not contain agricultural chemicals and sterilant, be suitable for advantages such as suitability for industrialized production, so the liquid submerged fermentation technology will progressively replace artificial culture, become the main mode of producing glossy ganoderma from now on.But up to the present also few to the research of glossy ganoderma industrial fermentation, some small-scale fermentation techniques are only arranged, lack the large scale fermentation Study on Technology.Particularly when fermentation was increased to certain scale, various parameter control played considerable effect to the quality even the success or failure of whole fermentation.The fermentation of different scales, required Comprehensive Control parameter difference if the direct cover of zymotechnique is used for large scale fermentation on a small scale, tends to occur parameter control imbalance, is directed at a large amount of mycelium agings or self-dissolving.Make mycelium production very low, and of poor quality, do not reach industrial production requirement.Just because of above-mentioned reason, cause existing glossy ganoderma fermentation production all to be confined to less scale (below 10 tons), this is difficult to satisfy market and ends. the demand growing to glossy ganoderma.
The objective of the invention is by research and improvement glossy ganoderma fermentation technology, the ganoderma lucidum liquid deep layer fermenting process of a kind of extensive (18 tons) is provided, output, productive rate and the quality of Ganoderma mycelium all are improved significantly, thereby solve the existing the problems referred to above of prior art.
Process for fermenting lucid ganoderma in 18-ton fermentor of the present invention may further comprise the steps:
(1) the glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 22-28 ℃, cultivated 72-96 hour on 160-220 rev/min the shaking table;
(2) bacterial classification in two bottles of 2L triangular flasks is transferred 30L is female to plant in jar (fermention medium is housed), transfer pH to 5.0-7.0, keep jar warm 24-28 ℃, tank pressure 10-40kPa, stir speed (S.S.) 80-120 rev/min, air flow 1: 0.1-0.4 fermented 36-60 hour;
(3) mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 5.0-7.0, keep jar warm 24-28 ℃, tank pressure 15-60kPa, stir speed (S.S.) 90-140 rev/min, air flow 1: 0.2-0.6 fermented 36-48 hour;
(4) bacterial classification in the first class seed pot is transferred in one 3 tons the secondary seed jar (fermention medium is housed), transfer pH to 5.0-7.0, keep jar warm 22-28 ℃, tank pressure 25-70kPa, stir speed (S.S.) 140-200 rev/min, air flow 1: 0.4-0.8 fermented 48-72 hour;
(5) bacterial classification in the secondary seed jar is transferred in one 18 tons the fermentor tank (fermention medium is housed), transfer pH to 5.0-7.0, keep warm 22-28 ℃ in jar, tank pressure 20-40kpa, stir speed (S.S.) 180-250 rev/min, air flow 1: 0.6-1.2, fermented 72-120 hour, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.
Jar standard of putting that this technology is taked is:
1, thalli morphology: microscopy has a large amount of mycelium, no tangible bud head and clamp connexion on the Ganoderma lucidum mycelium, and mycelia attenuates, the aggegation of mycelia protoplasma, the fermented liquid very thickness that becomes, and most mycelium senesces, and the minority mycelia has autolysis;
2, potential of hydrogen: put jar when the general pH value drops between the 2.5-3.0.
The composition of used shake-flask culture base and proportioning thereof are by weight percentage in the zymotechnique of the present invention: potato 20, glucose 2, sal epsom 0.02, potassium primary phosphate 0.1, vitamins B
10.005 all the other are water.
The composition of used fermention medium and proportioning thereof are by weight percentage in the zymotechnique of the present invention: soybean cake powder 1-3, wheat bran 0.1-1.0, Semen Maydis powder 2-4, glucose 2-5, peptone 0.5-1.5, sal epsom 0.02-0.04, potassium primary phosphate 0.1-0.3, vitamins B
10.001-0.005 all the other are water.
Zymotechnique of the present invention is suitable and in existing various Ganderma lucidum strains.
The present invention is by the comprehensive improvement to glossy ganoderma fermentation technology, 18 tons of extensive deep glossy ganoderma fermenting technologies of jar have successfully been set up, solved extensive in the past low, the ropy problem of glossy ganoderma fermentation productive rate, glossy ganoderma fermentation is only limited to the limitation of (below 10 tons) on a small scale thereby broken in the past, and this has great importance to the development that promotes glossy ganoderma fermentation and glossy ganoderma and be applied to industry.Technology one time fermentation scale of the present invention promptly reaches 18 tons, improved the yield in unit time of glossy ganoderma fermentation greatly, simultaneously, productive rate (mycelial yield of unit substratum) and mycelia quality all are improved significantly, reached the mycelia dry powder yield of 1.0-1.5%, the ganoderma polyoses content of mycelia dry powder reaches every hectogram mycelia dry powder 7-9g, all is significantly increased than in the past technology.
The invention will be further described by the following examples:
Embodiment 1: fresh glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 24 ℃, cultivated 72 hours on 180 rev/mins the shaking table.The 30L that then bacterial classification in two bottles of 2L triangular flasks transferred is female to plant in jar (fermention medium is housed), transfers pH to 6.0, keeps 24 ℃ of jar temperature, tank pressure 10kPa, and 120 rev/mins of stir speed (S.S.)s, air flow 1: 0.1 fermented 48 hours.Again mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 5.8, keep 24 ℃ of jar temperature, tank pressure 20kPa, 130 rev/mins of stir speed (S.S.)s, air flow 1: 0.2 fermented 36 hours.Then bacterial classification in the first class seed pot is transferred in one 3 tons the second order fermentation jar (fermention medium is housed), transfer pH to 5.5, keep a jar warm 24C, tank pressure 25kPa, 160 rev/mins of stir speed (S.S.)s, air flow 1: 0.8 fermented 72 hours.At last the bacterial classification in the secondary seed jar is transferred in one the 18 tons fermentor tanks (fermention medium is housed), transfer pH to 6.0, keep 24 ℃ of jar temperature, tank pressure 30kPa, 180 rev/mins of stir speed (S.S.)s, air flow 1: 0.6 fermented 108 hours, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.Pulverize wet mycelium oven dry back, obtains mycelia dry powder, and its yield is 1.26%.The mycelia dry powder sample that takes a morsel, the content that records ganoderan in the dry powder according to the phenolsulfuric acid method is 8.56g/100g glossy ganoderma dry powder.
Embodiment 2: fresh glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 27 ℃, cultivated 72 hours on 160 rev/mins the shaking table.The 30L that then bacterial classification in two bottles of 2L triangular flasks transferred is female to plant in jar (fermention medium is housed), transfers pH to 6.5, keeps 27 ℃ of jar temperature, tank pressure 30kPa, and 110 rev/mins of stir speed (S.S.)s, air flow 1: 0.4 fermented 36 hours.Again mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 6.0, keep 27 ℃ of jar temperature, tank pressure 40kPa, 140 rev/mins of stir speed (S.S.)s, air flow 1: 0.6 fermented 48 hours.Then bacterial classification in the first class seed pot is transferred in one 3 tons the second order fermentation jar (fermention medium is housed), transfer pH to 6.5, keep 27 ℃ of jar temperature, tank pressure 55kPa, 180 rev/mins of stir speed (S.S.)s, air flow 1: 0.8 fermented 72 hours.At last the bacterial classification in the secondary seed jar is transferred in one the 18 tons fermentor tanks (fermention medium is housed), transfer pH to 6.0, keep 27 ℃ of jar temperature, tank pressure 40kPa, 200 rev/mins of stir speed (S.S.)s, air flow 1: 1 fermented 84 hours, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.Pulverize wet mycelium oven dry back, obtains mycelia dry powder, and its yield is 1.06%.The mycelia dry powder sample that takes a morsel, the content that records ganoderan in the dry powder according to the phenolsulfuric acid method is 7.78g/100g glossy ganoderma dry powder.
Embodiment 3: fresh glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 28 ℃, cultivated 84 hours on 170 rev/mins the shaking table.The 30L that then bacterial classification in two bottles of 2L triangular flasks transferred is female to plant in jar (fermention medium is housed), transfers pH to 5.5, keeps 28 ℃ of jar temperature, tank pressure 20kPa, and 180 rev/mins of stir speed (S.S.)s, air flow 1: 0.3 fermented 48 hours.Again mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 6.0, keep 28 ℃ of jar temperature, tank pressure 20kPa, 130 rev/mins of stir speed (S.S.)s, air flow 1: 0.2 fermented 36 hours.Then bacterial classification in the first class seed pot is transferred in one 3 tons the second order fermentation jar (fermention medium is housed), transfer pH to 6.0, keep 28 ℃ of jar temperature, tank pressure 30kPa, 180 rev/mins of stir speed (S.S.)s, air flow 1: 0.6 fermented 60 hours.At last the bacterial classification in the secondary seed jar is transferred in one the 18 tons fermentor tanks (fermention medium is housed), transfer pH to 5.5, keep 28 ℃ of jar temperature, tank pressure 20kPa, 220 rev/mins of stir speed (S.S.)s, air flow 1: 0.8 fermented 120 hours, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.Pulverize wet mycelium oven dry back, obtains mycelia dry powder, and its yield is 1.45%.The mycelia dry powder sample that takes a morsel, the content that records ganoderan in the dry powder according to the phenolsulfuric acid method is 8.15g/100g glossy ganoderma dry powder.
Embodiment 4: fresh glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 25 ℃, cultivated 96 hours on 200 rev/mins the shaking table.The 30L that then bacterial classification in two bottles of 2L triangular flasks transferred is female to plant in jar (fermention medium is housed), transfers pH to 6.5, keeps 25 ℃ of jar temperature, tank pressure 40kPa, and 100 rev/mins of stir speed (S.S.)s, air flow 1: 0.2 fermented 48 hours.Again mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 6.5, keep 25 ℃ of jar temperature, tank pressure 30kPa, 120 rev/mins of stir speed (S.S.)s, air flow 1: 0.4 fermented 36 hours.Then bacterial classification in the first class seed pot is transferred in one 3 tons the second order fermentation jar (fermention medium is housed), transfer pH to 6.0, keep 25 ℃ of jar temperature, tank pressure 35kPa, 160 rev/mins of stir speed (S.S.)s, air flow 1: 0.7 fermented 60 hours.At last the bacterial classification in the secondary seed jar is transferred in one the 18 tons fermentor tanks (fermention medium is housed), transfer pH to 5.8, keep 25 ℃ of jar temperature, tank pressure 30kPa, 200 rev/mins of stir speed (S.S.)s, air flow 1: 0.8 fermented 108 hours, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.Pulverize wet mycelium oven dry back, obtains mycelia dry powder, and its yield is 1.35%.The mycelia dry powder sample that takes a morsel, the content that records ganoderan in the dry powder according to the phenolsulfuric acid method is 8.45g/100g glossy ganoderma dry powder.
Embodiment 5: fresh glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 26 ℃, cultivated 76 hours on 180 rev/mins the shaking table.The 30L that then bacterial classification in two bottles of 2L triangular flasks transferred is female to plant in jar (fermention medium is housed), transfers pH to 6.0, keeps 26 ℃ of jar temperature, tank pressure 15kPa, and 110 rev/mins of stir speed (S.S.)s, air flow 1: 0.2 fermented 48 hours.Again mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 6.0, keep 26 ℃ of jar temperature, tank pressure 25kPa, 140 rev/mins of stir speed (S.S.)s, air flow 1: 0.3 fermented 36 hours.Then bacterial classification in the first class seed pot is transferred in one 3 tons the second order fermentation jar (fermention medium is housed), transfer pH to 5.5, keep 26 ℃ of jar temperature, tank pressure 35kPa, 160 rev/mins of stir speed (S.S.)s, air flow 1: 0.7 fermented 72 hours.At last the bacterial classification in the secondary seed jar is transferred in one the 18 tons fermentor tanks (fermention medium is housed), transfer pH to 6.0, keep 26 ℃ of jar temperature, tank pressure 35kPa, 180 rev/mins of stir speed (S.S.)s, air flow 1: 0.7 fermented 120 hours, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.Pulverize wet mycelium oven dry back, obtains mycelia dry powder, and its yield is 1.20%.The mycelia dry powder sample that takes a morsel, the content that records ganoderan in the dry powder according to the phenolsulfuric acid method is 7.69g/100g glossy ganoderma dry powder.
Claims (3)
1, a kind of process for fermenting lucid ganoderma in 18-ton fermentor, its feature may further comprise the steps:
(1) the glossy ganoderma slant strains is inserted respectively in two 2L triangular flasks (the shake-flask culture base is housed), under 22-28 ℃, cultivated 72-96 hour on 160-220 rev/min the shaking table;
(2) bacterial classification in two bottles of 2L triangular flasks is transferred 30L is female to plant in jar (fermention medium is housed), transfer pH to 5.0-7.0, keep jar warm 24-28 ℃, tank pressure 10-40kPa, stir speed (S.S.) 80-120 rev/min, air flow 1: 0.1-0.4 fermented 36-60 hour;
(3) mother is planted bacterial classification in the jar and transfer in the first class seed pot (fermention medium is housed) of a 300L, transfer pH to 5.0-7.0, keep jar warm 24-28 ℃, tank pressure 15-60kPa, stir speed (S.S.) 90-140 rev/min, air flow 1: 0.2-0.6 fermented 36-48 hour;
(4) bacterial classification in the first class seed pot is transferred in one 3 tons the secondary seed jar (fermention medium is housed), transfer pH to 5.0-7.0, keep jar warm 22-28 ℃, tank pressure 25-70kPa, stir speed (S.S.) 140-200 rev/min, air flow 1: 0.4-0.8 fermented 48-72 hour;
(5) bacterial classification in the secondary seed jar is transferred in one 18 tons the fermentor tank (fermention medium is housed), transfer pH to 5.0-7.0, keep warm 22-28 ℃ in jar, tank pressure 20-40kPa, stir speed (S.S.) 180-250 rev/min, air flow 1: 0.6-1.2, fermented 72-120 hour, put jar then,, get the glossy ganoderma wet mycelium the fermented liquid press filtration.
2, method described in the claim 1 is characterized in that the composition of used shake-flask culture and proportioning thereof are by weight percentage: potato 20, glucose 2, sal epsom 0.02, potassium primary phosphate 0.1, vitamins B
10.005 all the other are water.
3, method described in the claim 1 is characterized in that the composition of used fermention medium and proportioning thereof are by weight percentage: soybean cake powder 1-3, wheat bran 0.1-1.0, Semen Maydis powder 2-4, glucose 2-5, peptone 0.5-1.5, sal epsom 0.02-0.04, potassium primary phosphate 0.1-0.3, vitamins B
10.001-0.005 all the other are water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99113515 CN1266897A (en) | 1999-03-10 | 1999-03-10 | Process for fermenting lucid ganoderma in 18-ton fermentor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 99113515 CN1266897A (en) | 1999-03-10 | 1999-03-10 | Process for fermenting lucid ganoderma in 18-ton fermentor |
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| CN1266897A true CN1266897A (en) | 2000-09-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267398A (en) * | 2017-06-16 | 2017-10-20 | 江阴市长泾国民育种场 | A kind of Liquid Strain of Ganoderma Lucidum culture medium prescription and Liquid Strain of Ganoderma Lucidum cultural method |
-
1999
- 1999-03-10 CN CN 99113515 patent/CN1266897A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267398A (en) * | 2017-06-16 | 2017-10-20 | 江阴市长泾国民育种场 | A kind of Liquid Strain of Ganoderma Lucidum culture medium prescription and Liquid Strain of Ganoderma Lucidum cultural method |
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