CN109200214A - A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof - Google Patents
A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof Download PDFInfo
- Publication number
- CN109200214A CN109200214A CN201811208631.7A CN201811208631A CN109200214A CN 109200214 A CN109200214 A CN 109200214A CN 201811208631 A CN201811208631 A CN 201811208631A CN 109200214 A CN109200214 A CN 109200214A
- Authority
- CN
- China
- Prior art keywords
- highland barley
- fermentation
- ganoderma
- bud
- ganoderma lucidum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 215
- 238000000855 fermentation Methods 0.000 title claims abstract description 131
- 230000004151 fermentation Effects 0.000 title claims abstract description 131
- 241000222336 Ganoderma Species 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 240000005979 Hordeum vulgare Species 0.000 title description 155
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 74
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 73
- 241000209219 Hordeum Species 0.000 claims abstract description 61
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 150000004676 glycans Chemical class 0.000 claims abstract description 28
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 28
- 239000005017 polysaccharide Substances 0.000 claims abstract description 28
- 239000012535 impurity Substances 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 10
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
- 150000001413 amino acids Chemical class 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 150000007524 organic acids Chemical class 0.000 claims abstract description 8
- 235000005985 organic acids Nutrition 0.000 claims abstract description 6
- 239000002609 medium Substances 0.000 claims description 33
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 21
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 17
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- 238000009835 boiling Methods 0.000 claims description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 9
- 241001480597 Ganoderma tsugae Species 0.000 claims description 8
- 102000004139 alpha-Amylases Human genes 0.000 claims description 7
- 108090000637 alpha-Amylases Proteins 0.000 claims description 7
- 229940024171 alpha-amylase Drugs 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 7
- 238000000967 suction filtration Methods 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 235000015277 pork Nutrition 0.000 claims description 6
- 241001460390 Ganoderma lipsiense Species 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 150000003863 ammonium salts Chemical group 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 244000000231 Sesamum indicum Species 0.000 claims 2
- 235000013305 food Nutrition 0.000 abstract description 7
- 239000003814 drug Substances 0.000 abstract description 6
- 239000004615 ingredient Substances 0.000 abstract description 5
- 239000002537 cosmetic Substances 0.000 abstract description 4
- 241000207961 Sesamum Species 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 241000233866 Fungi Species 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 5
- 230000002929 anti-fatigue Effects 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000010563 solid-state fermentation Methods 0.000 description 4
- 239000004382 Amylase Substances 0.000 description 3
- 102000013142 Amylases Human genes 0.000 description 3
- 108010065511 Amylases Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000640636 Ganoderma leucocontextum Species 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 235000019418 amylase Nutrition 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 241001489091 Ganoderma sinense Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000002218 hypoglycaemic effect Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 239000010985 leather Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000192128 Gammaproteobacteria Species 0.000 description 1
- 241000390476 Ganoderma lingzhi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001646834 Mesona Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000003471 anti-radiation Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 230000027288 circadian rhythm Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/20—Malt products
- A23L7/25—Fermentation of cereal malt or of cereal by malting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Biochemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to fermentation engineering fields, and in particular to a kind of highland barley glossy ganoderma fermentation magma and preparation method thereof.The method prepares highland barley fermentation medium by raw material of highland barley bud, and Ganoderma Lucidum is accessed in the highland barley fermentation medium and is fermented, highland barley ganoderma lucidum fermented liquid is obtained, and after the removal of impurity twice, obtains highland barley glossy ganoderma fermentation magma.Polysaccharide is rich in the highland barley glossy ganoderma fermentation magma prepared, its polysaccharide a part derives from highland barley, another part derives from ganoderma lucidum, furthermore can be applied to the fields such as food, medicine, cosmetics also containing ingredients such as amino acid, protein, organic acids in highland barley glossy ganoderma fermentation magma.
Description
Technical field
The invention belongs to fermentation engineering fields, and in particular to a kind of highland barley glossy ganoderma fermentation magma and preparation method thereof.
Background technique
Highland barley (Hordeum vulgare L.var.nudum Hook.f.) is a kind of grain work of grass family, Hordeum
Object is distributed mainly on Tibet, Qinghai, Gansu, Sichuan Aba and the high altitude localities such as Ganzi and Bolivia, because its
Caryopsis and bran shell are isolated name when harvest, are also referred to as barley, wheat, meter Mai, naked barley in other producing regions.Highland barley is in cereal
Good merchantable brand, edible value and nutritive value with higher are rich in polysaccharide, protein, fat, dietary fiber and various trace elements
Equal ingredients, the content of polysaccharide are 3.66%~8.62%, and average content 5.25% is much higher than barley, wheat and oat, is
The peak of polysaccharide in cereal crops is measured so far.Highland barley, which has, improves the total defense energy of body in particular circumstances
Power adjusts circadian rhythm, anti anoxia, antifatigue, reducing blood lipid, hypoglycemic, prevention cardio-cerebrovascular diseases unique healthcare function,
It is a kind of comparatively ideal drug or health-care food raw material.
Ganoderma lucidum is a kind of medicinal fungi of preciousness, is just respectfully called refreshing sesame, mesona in ancient times, and ganoderma lucidum belongs to load in classification
Gammaproteobacteria Polyporaceae Ganoderma common are red sesame (Ganoderma lucidum), purple sesame (G.sinense), Ganoderma tsugae
(G.tsugae Murr.), ganoderma lipsiense (G.aplanatum), plain boiled pork ganoderma lucidum (G.leucocontextum) etc..The Ministry of Public Health exists
It include three kinds of red sesame, purple sesame and Ganoderma tsugae spirits in " the fungi strain list that can be used for health food " of publication in 2001
Sesame category fungi.The nutritive value and medical value of ganoderma lucidum are very high, are strengthening by means of tonics, the good merchantable brand strengthened the body resistance to consolidate the constitution, have anti-stop
Arteries and veins hardening improves a variety of pharmacological activity such as immunity, antitumor, anti-aging, anti-oxidant, antifatigue, antiviral, has clinically used
In the treatment of a variety of diseases, U.S.'s herbal medicine pharmacopeia has been put into it.
Polysaccharide (Polysaccharides) is as 10 or more monosaccharide by containing aldehyde radical or ketone group made of glucosides key connection
Natural polymers, be constitute life one of four big base substances.According to source, it is more polysaccharide can be divided into animal
Sugar, plant polyose, bacterial polysaccharides, fungi polysaccharide and algal polysaccharides.For many years studies have shown that polysaccharose substance have it is anti-swollen
The multiple biological activities such as tumor, immunological regulation, hypoglycemic, antiviral, anti-oxidant, anti-radiation, antifatigue, in food, medicine, makeup
The fields such as product have a wide range of applications.The polysaccharide of separate sources has different bioactivity, and general fungi polysaccharide has
Higher anti-tumor activity, and plant polyose then has preferable immunologic enhancement.Therefore, fungi polysaccharide and plant in recent years
Polysaccharide is gradually taken seriously.
In existing application technology, the improvement method [(open (bulletin) of polyoses content in the highland barley based on solid fermentation
Number: CN104087633A] in, disclose a kind of method using Ganoderma Lucidum solid state fermentation highland barley, this method deficiency first is that adopting
With solid state fermentation, cause that fermentation period is long, fermentation is uneven, yield of active ingredients is limited, deficiency second is that fermenting after also need
Separation is extracted to active constituent.Another patent, a kind of highland barley fermentation magma and preparation method and application [open (bulletin)
Number: CN107308073A] in, a kind of method for preparing highland barley fermentation magma is disclosed, which utilizes using highland barley bran as raw material
Saccharomycetes to make fermentation obtains beta glucan and other active components, this method deficiency first is that highland barley bran lacks hullessbarley seed
The nutritional ingredients such as protein, amino acid, and carbon source and nitrogen source content are lower in highland barley bran, are unfavorable for the growth of microorganism, no
Foot second is that using saccharomycetes to make fermentation, a certain amount of ethyl alcohol can be generated, to human body have certain stimulation, in food, medicine, change
The application in the fields such as cosmetic has certain limitation.
Summary of the invention
In view of the deficienciess of the prior art, the present invention provides a kind of highland barley glossy ganoderma fermentation magma and preparation method thereof, mesh
One of be to provide a kind of highland barley glossy ganoderma fermentation magma, in the highland barley glossy ganoderma fermentation magma be rich in polysaccharide, polysaccharide a part come
Derived from highland barley, another part derives from ganoderma lucidum, and amino acid, protein, organic acid are furthermore also contained in highland barley glossy ganoderma fermentation magma
Equal ingredients, can be applied to the fields such as food, medicine, cosmetics.
The second object of the present invention is to provide a kind of preparation method of above-mentioned highland barley glossy ganoderma fermentation magma.
The present invention is achieved by the following technical solutions:
A kind of preparation method of highland barley glossy ganoderma fermentation magma prepares highland barley fermentation medium by raw material of highland barley bud, will be clever
Sesame bacterium, which is accessed in the highland barley fermentation medium, ferments, and obtains highland barley ganoderma lucidum fermented liquid, after the removal of impurity twice, obtains
Obtain highland barley glossy ganoderma fermentation magma.
Further, the preparation method of the highland barley fermentation medium specifically:
It crushes: by the drying of highland barley bud, removing root, then crushed 40 meshes, obtain highland barley bud powder；
Saccharification: the highland barley bud powder and water are mixed according to volume ratio 1:(4-10), then press highland barley bud powder weight
0.05-0.08% be added alpha-amylase, 60-80 DEG C heat preservation 4-10 hours, obtain highland barley bud juice；
Boiling: nitrogen source is added into the highland barley bud juice according to mass ratio 0.1%-0.5%, then at 100-121 DEG C
Boiling 15-20 minutes, highland barley fermentation medium is obtained, it is spare after the highland barley fermentation medium is cooled to 25-30 DEG C.
The technical effect of the preparation method of above-mentioned highland barley fermentation medium are as follows: highland barley is crushed, can be increased in highland barley
The active area of starch granules and amylase is added amylase and is digested under the optimal condition of amylase, can be made
Starch in highland barley is further efficiently converted into the glucose that Ganoderma Lucidum most easily utilizes, to effectively shorten the hair of subsequent Ganoderma Lucidum
The ferment period improves production efficiency.
Further, Ganoderma Lucidum is accessed in the highland barley fermentation medium and is fermented 3-5 days, obtain highland barley glossy ganoderma fermentation
Liquid；
During the fermentation, highland barley fermentation medium is stood, stirred or divulged information；Upon agitation, control mixing speed is
150rpm-200rpm；When ventilation, control ventilation quantity is 120-150L/h；During the fermentation, control dissolved oxygen is 25%-
30%.Preferably, control dissolved oxygen is 25% or 30%.Ganoderma lucidum Pseudomonas obligate aerobes, thus in fermentation liquid oxygen concentration pair
Extremely important in the growth of Ganoderma Lucidum, ventilation is the supply in order to increase oxygen, and stirring is to make the oxygen in air
It being preferably scattered in fluid nutrient medium, is found through a large number of experiments, above-mentioned fermentation condition is the optimum growing condition of Ganoderma Lucidum,
Under this condition, a large amount of proliferation for being conducive to Ganoderma Lucidum, promote the generation of ganoderma lucidum polysaccharide, while being also beneficial to Ganoderma Lucidum degradation highland barley
Cell wall structure, promote highland barley in polysaccharide dissolution.
Further, highland barley ganoderma lucidum fermented liquid is carried out impurity twice to remove, is specifically included: after terminating fermentation, by highland barley
Ganoderma lucidum fermented liquid is centrifuged, and to remove the impurity such as the insoluble granule in fermentation liquid, completes removal impurity for the first time；Then it presses
Diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid, after being mixed 30 minutes, filters outstanding to remove
Floating or tannin, polyphenol, large molecular weight protein for being dissolved in fermentation liquid etc., to guarantee that fermentation magma will not in long-term preservation
Phenomena such as generating flocculation, muddiness achievees the purpose that purification and improves finished product preparation quality, completes second of removal impurity, suction filtration
Highland barley glossy ganoderma fermentation magma is obtained afterwards.
Further, the highland barley bud the preparation method comprises the following steps: under conditions of 15-20 DEG C, it is that highland barley seed is clean
Water impregnates 2-5 hours, removes excessive moisture, then keeps 20-30 hours wet at 20-25 DEG C, then be placed under natural conditions
It places 3-4 days, obtains highland barley bud.Wherein, used highland barley seed is belt leather highland barley, i.e., the hullessbarley seed that do not peel.
Further, the nitrogen source being added into the highland barley bud juice is ammonium salt, yeast extract or peptone.
Further, the Ganoderma Lucidum is using red sesame, purple sesame, Ganoderma tsugae, Chinese ganoderma lucidum, ganoderma lipsiense, plain boiled pork ganoderma lucidum
One of.
Further, the method specifically includes:
(1) preparation of highland barley bud: under conditions of 15-20 DEG C, the water of highland barley seed cleaning is impregnated 2-5 hours, is gone
Except excessive moisture, then keeps 20-30 hours wet at 20-25 DEG C, then be placed under natural conditions and place 3-4 days, obtain blueness
Highland barley bud；
(2) it crushes: highland barley bud being dried at 60 DEG C, root is removed, after crushed 40 meshes, obtains highland barley bud powder；
(3) it is saccharified: the highland barley bud powder and water is mixed according to volume ratio 1:(4-10), then press highland barley bud powder weight
Alpha-amylase is added in the 0.05-0.08% of amount, 60-80 DEG C heat preservation 4-10 hours, obtain highland barley bud juice；
(4) nitrogen source boiling: is added into the highland barley bud juice according to mass ratio 0.1%-0.5% then at 100-121 DEG C
Lower boiling 15-20 minutes, obtains highland barley fermentation medium；
(5) it ferments: after fermentation medium is cooled to 25-30 DEG C, ganoderma lucidum being accessed into highland barley fermentation medium the 3-5 that ferments
It, obtains highland barley ganoderma lucidum fermented liquid.
(6) it cleans: after terminating fermentation, highland barley ganoderma lucidum fermented liquid being centrifuged twice, complete the removal of first time impurity；So
Diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid afterwards, after being mixed 30 minutes, filters and completes
Second of impurity removes, and highland barley glossy ganoderma fermentation magma is obtained after suction filtration.
A kind of highland barley glossy ganoderma fermentation magma, using a kind of preparation method preparation of highland barley glossy ganoderma fermentation magma, by every
Gram highland barley glossy ganoderma fermentation magma calculates, and every gram of highland barley glossy ganoderma fermentation magma includes: 25.2-36.1mg polysaccharide, 4.3-
11.6mg protein, 1.8-4.3mg total amino acid and 2.5-5.3mg total organic acids.
Advantageous effects of the invention:
1) relative to solid state fermentation, the method for the invention uses liquid state fermentation, has fermentation period short, raw material availability
Height, fermentation condition is easily controllable and standardizes, and tunning can carry out using directly as product after filtering clarification, reduce
Pollution to environment.
Also, polysaccharide is dissolvable in water in the fermentation liquid of liquid fermentation, after cleaning as a kind of water-soluble active material
The aqueous solution that polysaccharide can be obtained, does not need additional separating step, and the inert matter after solid fermentation, in tunning
Accounting example is very high, is such as not isolated, and will seriously affect the Function of active material.
2) with respect to the background art employed in highland barley bran for ferment when, the method for the invention use highland barley
Bud, highland barley bud, containing nutriments such as more abundant starch, albumen, are more advantageous to the growth of Ganoderma Lucidum compared with wheat bran
And breeding, it is easier to it is utilized by Ganoderma Lucidum, it is shorter so as to shorten fermentation period, improve production efficiency.
3) present invention is fermented using Ganoderma Lucidum, and Ganoderma Lucidum is a kind of fungi, is nearly free from second during the growth process
Alcohol, and yeast is in the food industry, to use ethyl alcohol conversion effect with higher mainly as a kind of industrial strain of producing and ethanol
Rate.
4) a kind of highland barley glossy ganoderma fermentation magma prepared using the method for the invention is rich in active polysaccharide, polysaccharide
A part derives from highland barley, and another part derives from ganoderma lucidum, and amino acid, albumen are furthermore also contained in highland barley glossy ganoderma fermentation magma
The ingredients such as matter, organic acid have the effects that anti-oxidant, antifatigue, anti-aging, can be applied to the neck such as food, medicine, cosmetics
Domain.
5) a kind of highland barley glossy ganoderma fermentation magma provided by the present invention is sent out using the hullessbarley seed that do not peel as raw material
Bud obtains highland barley bud and then prepares highland barley fermentation medium, is inoculated with Ganoderma fungi, directly obtains highland barley spirit by fermentation
Sesame fermentation magma, has many advantages, such as that preparation process is simple, with short production cycle, Green-pollution is small.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is explained in further detail.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, it is not used to
Limit the present invention.
On the contrary, the present invention covers any substitution done on the essence and scope of the present invention being defined by the claims, repairs
Change, equivalent method and scheme.Further, in order to make the public have a better understanding the present invention, below to of the invention thin
It is detailed to describe some specific detail sections in section description.Part without these details for a person skilled in the art
The present invention can also be understood completely in description.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Highland barley as used in the following examples is the highland barley that do not peel, i.e. belt leather highland barley, picks up from Tibet, Qinghai or Gansu
Equal provinces and regions.
Ganoderma lucidum as used in the following examples is the bacterial strain of following type: red sesame (Ganoderma lucidum), purple sesame
(G.sinense), Ganoderma tsugae (G.tsugae Murr.), Chinese ganoderma lucidum (G.lingzhi), ganoderma lipsiense
(G.aplanatum), plain boiled pork ganoderma lucidum (G.leucocontextum).Each bacterial strain is purchased from Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center (CGMCC).
Unevenly, yield of active ingredients is limited, after fermentation for, fermentation long for solid state fermentation fermentation period in the prior art
Also need the technical issues of separation is extracted to active constituent；And in the prior art using highland barley bran as fermentation raw material
The technical issues of being unfavorable for fermentative microorganism growth, ethyl alcohol generated using saccharomycetes to make fermentation；
The present embodiment provides a kind of preparation methods of highland barley glossy ganoderma fermentation magma, prepare highland barley fermentation by raw material of highland barley bud
Ganoderma Lucidum is accessed in the highland barley fermentation medium and is fermented, obtains highland barley ganoderma lucidum fermented liquid by culture medium, and process is miscellaneous twice
After matter removal, highland barley glossy ganoderma fermentation magma is obtained.
In the present embodiment, the preparation method of the highland barley fermentation medium specifically:
It crushes: by the drying of highland barley bud, removing root, then crushed 40 meshes, obtain highland barley bud powder；
Saccharification: the highland barley bud powder and water are mixed according to volume ratio 1:(4-10), small in 60-80 DEG C of heat preservation 4-10
When, obtain highland barley bud juice；
Boiling: nitrogen source is added into the highland barley bud juice according to mass ratio 0.1%-0.5%, then at 100-121 DEG C
Boiling 15-20 minutes, highland barley fermentation medium is obtained, it is spare after the highland barley fermentation medium is cooled to 25-30 DEG C.
In the present embodiment, Ganoderma Lucidum is accessed in the highland barley fermentation medium and is fermented 3-5 days, obtain highland barley ganoderma lucidum hair
Zymotic fluid；During the fermentation, highland barley fermentation medium is stood, stirred or divulged information；Upon agitation, control mixing speed is
150rpm-200rpm；When ventilation, control ventilation quantity is 120-150L/h；During the fermentation, control dissolved oxygen is 25%-
30%.
In the present embodiment, highland barley ganoderma lucidum fermented liquid is carried out impurity twice to remove, is specifically included:, will after terminating fermentation
Highland barley ganoderma lucidum fermented liquid is centrifuged, and removal impurity for the first time is completed；Then 2g diatom is added by every 1 liter of highland barley ganoderma lucidum fermented liquid
Diatomite is added in the ratio of soil, after being mixed 30 minutes, filters and completes second of removal impurity, obtain highland barley ganoderma lucidum after suction filtration
Fermentation magma.
In the present embodiment, a kind of preparation method of highland barley bud is provided, specifically: under conditions of 15-20 DEG C, by highland barley
The water of seed cleaning impregnates 2-5 hours, removes excessive moisture, then keeps 20-30 hours wet at 20-25 DEG C, then set
It is placed 3-4 days under natural conditions, obtains highland barley bud.
Specifically, the nitrogen source being added in the present embodiment into the highland barley bud juice is ammonium salt, yeast extract or peptone.
Preferably, the Ganoderma Lucidum is using in red sesame, purple sesame, Ganoderma tsugae, Chinese ganoderma lucidum, ganoderma lipsiense, plain boiled pork ganoderma lucidum
One kind.
A kind of highland barley glossy ganoderma fermentation magma prepared using the present embodiment the method, by every gram of highland barley glossy ganoderma fermentation
Magma calculates, and every gram of highland barley glossy ganoderma fermentation magma includes: 25.2-36.1mg polysaccharide, 4.3-11.6mg protein, 1.8-
4.3mg total amino acid and 2.5-5.3mg total organic acids.
Another embodiment of the present invention provides a kind of preparation method of highland barley glossy ganoderma fermentation magma, the method is specifically included:
(1) preparation of highland barley bud: under conditions of 20 DEG C, the water of highland barley seed cleaning is impregnated 3 hours, it is extra to remove
Then moisture keeps 20-30 hours wet at 20 DEG C, then is placed under natural conditions and places 3 days, obtain highland barley bud；
(2) it crushes: highland barley bud being dried at 60 DEG C, root is removed, after crushed 40 meshes, obtains highland barley bud powder；
(3) it is saccharified: the highland barley bud powder and water is mixed according to volume ratio 1:10, then by highland barley bud powder weight
Alpha-amylase is added in 0.05-0.08%, is then added and alpha-amylase is added by the 0.08% of highland barley bud powder weight, be warming up to 70
DEG C, 30 minutes are kept the temperature, continues heat preservation 4 hours, obtains highland barley bud juice；
(4) nitrogen source boiling: is added into the highland barley bud juice according to mass ratio 0.3% (using ammonium chloride in the present embodiment
As nitrogen source), it is then heated to boiling, and keep the temperature 20 minutes, obtains highland barley fermentation medium；
(5) it ferments: after fermentation medium is cooled to 30 DEG C, red sesame (G.lucidum) being accessed into highland barley fermentation medium
Middle fermentation 4 days, obtains highland barley ganoderma lucidum fermented liquid；
(6) it cleans: after terminating fermentation, highland barley ganoderma lucidum fermented liquid being centrifuged under the conditions of 8000rpm twice, complete the
Impurity removal；Then diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid, is mixed 30
After minute, filters and complete second of impurity removal, obtain highland barley glossy ganoderma fermentation magma after suction filtration.
Through detecting, in the highland barley fermentation magma that the present embodiment obtains, contain in every 1 gram ganoderma lucidum highland barley fermentation magma
32.2-34.7mg polysaccharide, the protein of 7.3-8.4mg and 3.3-4.2mg total amino acid and 4.5-5.1mg total organic acids.
Another embodiment of the present invention provides a kind of preparation method of highland barley glossy ganoderma fermentation magma, the method is specifically included:
(1) preparation of highland barley bud: under conditions of 15 DEG C, the water of highland barley seed cleaning is impregnated 5 hours, it is extra to remove
Then moisture keeps 30 hours wet at 20 DEG C, then is placed under natural conditions and places 3 days, obtain highland barley bud；
(2) it crushes: highland barley bud being dried at 60 DEG C, root is removed, after crushed 40 meshes, obtains highland barley bud powder；
(3) it is saccharified: the highland barley bud powder and water is mixed according to volume ratio 1:5, be then added and press highland barley bud powder weight
Alpha-amylase is added in the 0.05% of amount, is warming up to 60 DEG C, keeps the temperature 30 minutes, continues heat preservation 8 hours, obtains highland barley bud juice；
(4) nitrogen source boiling: is added into the highland barley bud juice according to mass ratio 0.5% (using chlorination in the present embodiment
Ammonium), it is then heated to boiling, and keep the temperature 20 minutes, obtains highland barley fermentation medium；
(5) it ferments: after fermentation medium is cooled to 28 DEG C, plain boiled pork ganoderma lucidum (G.leucocontextum) being accessed into highland barley
It ferments 5 days in fermentation medium, obtains highland barley ganoderma lucidum fermented liquid；
(6) it cleans: after terminating fermentation, highland barley ganoderma lucidum fermented liquid being centrifuged under the conditions of 8000rpm twice, complete the
Impurity removal；Then diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid, is mixed 30
After minute, filters and complete second of impurity removal, obtain highland barley glossy ganoderma fermentation magma after suction filtration.
Through detecting, in the highland barley fermentation magma that the present embodiment obtains, contain in every 1 gram ganoderma lucidum highland barley fermentation magma
25.2-26.7mg polysaccharide, the protein of 4.3-5.2mg and 1.8-2.4mg total amino acid and 2.5-3.1mg total organic acids.
Claims (9)
1. a kind of preparation method of highland barley glossy ganoderma fermentation magma, which is characterized in that prepare highland barley fermentation training by raw material of highland barley bud
Base is supported, Ganoderma Lucidum is accessed in the highland barley fermentation medium and is fermented, highland barley ganoderma lucidum fermented liquid is obtained, by impurity twice
After removal, highland barley glossy ganoderma fermentation magma is obtained.
2. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1, which is characterized in that the highland barley fermentation
The preparation method of culture medium specifically:
It crushes: by the drying of highland barley bud, removing root, then crushed 40 meshes, obtain highland barley bud powder；
Saccharification: the highland barley bud powder and water are mixed according to volume ratio 1:(4-10), then by highland barley bud powder weight
0.05-0.08% be added alpha-amylase, 60-80 DEG C heat preservation 4-10 hours, obtain highland barley bud juice；
Boiling: nitrogen source is added into the highland barley bud juice according to mass ratio 0.1%-0.5%, the then boiling at 100-121 DEG C
15-20 minutes, highland barley fermentation medium is obtained, it is spare after the highland barley fermentation medium is cooled to 25-30 DEG C.
3. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1, which is characterized in that access Ganoderma Lucidum
It ferments 3-5 days in the highland barley fermentation medium, obtains highland barley ganoderma lucidum fermented liquid；
During the fermentation, highland barley fermentation medium is stood, stirred or divulged information；Upon agitation, control mixing speed is
150rpm-200rpm；When ventilation, control ventilation quantity is 120-150L/h；During the fermentation, control dissolved oxygen is 25%-
30%.
4. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1, which is characterized in that send out highland barley ganoderma lucidum
Zymotic fluid carries out impurity twice and removes, and specifically includes: after terminating fermentation, highland barley ganoderma lucidum fermented liquid being centrifuged, complete for the first time
Remove impurity；Then diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid, is mixed 30 minutes
Afterwards, it filters and completes second of removal impurity, obtain highland barley glossy ganoderma fermentation magma after suction filtration.
5. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1 or claim 2, which is characterized in that the highland barley
Bud the preparation method comprises the following steps: under conditions of 15-20 DEG C, the water of highland barley seed cleaning is impregnated 2-5 hour, removal superfluous water
Point, it then keeps 20-30 hours wet at 20-25 DEG C, then be placed under natural conditions and place 3-4 days, obtains highland barley bud.
6. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 2, which is characterized in that the highland barley bud
The nitrogen source being added in juice is ammonium salt, yeast extract or peptone.
7. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1, which is characterized in that the Ganoderma Lucidum is adopted
With one of red sesame, purple sesame, Ganoderma tsugae, Chinese ganoderma lucidum, ganoderma lipsiense, plain boiled pork ganoderma lucidum.
8. a kind of preparation method of highland barley glossy ganoderma fermentation magma according to claim 1, which is characterized in that the method is specific
Include:
(1) preparation of highland barley bud: under conditions of 15-20 DEG C, the water of highland barley seed cleaning is impregnated 2-5 hours, is removed more
Then remaining moisture keeps 20-30 hours wet at 20-25 DEG C, then is placed under natural conditions and places 3-4 days, obtain highland barley bud；
(2) it crushes: highland barley bud being dried at 60 DEG C, root is removed, after crushed 40 meshes, obtains highland barley bud powder；
(3) it is saccharified: the highland barley bud powder and water is mixed according to volume ratio 1:(4-10), then by highland barley bud powder weight
0.05-0.08% be added alpha-amylase, 60-80 DEG C heat preservation 4-10 hours, obtain highland barley bud juice；
(4) boiling: nitrogen source is added into the highland barley bud juice according to mass ratio 0.1%-0.5%, is then steamed at 100-121 DEG C
It boils 15-20 minutes, obtains highland barley fermentation medium；
(5) it ferments: after fermentation medium is cooled to 25-30 DEG C, ganoderma lucidum being accessed in highland barley fermentation medium and is fermented 3-5 days,
Obtain highland barley ganoderma lucidum fermented liquid.
(6) it cleans: after terminating fermentation, highland barley ganoderma lucidum fermented liquid being centrifuged twice, complete the removal of first time impurity；Then it presses
Diatomite is added in the ratio that 2g diatomite is added in every 1 liter of highland barley ganoderma lucidum fermented liquid, after being mixed 30 minutes, filters and completes second
Secondary impurity removes, and highland barley glossy ganoderma fermentation magma is obtained after suction filtration.
9. a kind of highland barley glossy ganoderma fermentation magma is prepared using any one of claim 1-7 the method, which is characterized in that by every
Gram highland barley glossy ganoderma fermentation magma calculates, and every gram of highland barley glossy ganoderma fermentation magma includes: 25.2-36.1mg polysaccharide, 4.3-
11.6mg protein, 1.8-4.3mg total amino acid and 2.5-5.3mg total organic acids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811208631.7A CN109200214A (en) | 2018-10-17 | 2018-10-17 | A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811208631.7A CN109200214A (en) | 2018-10-17 | 2018-10-17 | A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109200214A true CN109200214A (en) | 2019-01-15 |
Family
ID=64980732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811208631.7A Pending CN109200214A (en) | 2018-10-17 | 2018-10-17 | A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109200214A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114081862A (en) * | 2020-07-31 | 2022-02-25 | 伽蓝（集团）股份有限公司 | Preparation and application of saccharomyces cerevisiae fermentation product from highland barley wine yeast in pan-Himalaya region |
| CN115191551A (en) * | 2022-07-20 | 2022-10-18 | 四川省雅士科技有限公司 | Anoxia-resistant fermentation broth, preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018259A (en) * | 2010-12-03 | 2011-04-20 | 西藏月王生物技术有限公司 | Cordyceps sinensis and monacolin drink and preparation method thereof |
| CN104087633A (en) * | 2014-07-04 | 2014-10-08 | 西藏圣龙实业有限公司 | Method for improving content of polysaccharides in highland barley based on solid fermentation |
| CN105725182A (en) * | 2016-02-01 | 2016-07-06 | 中国人民解放军总后勤部军需装备研究所 | New application of schizophyllum commune fermentation broth |
| CN107308073A (en) * | 2017-06-26 | 2017-11-03 | 上海应用技术大学 | A kind of highland barley fermentation magma and preparation method and application |
-
2018
- 2018-10-17 CN CN201811208631.7A patent/CN109200214A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018259A (en) * | 2010-12-03 | 2011-04-20 | 西藏月王生物技术有限公司 | Cordyceps sinensis and monacolin drink and preparation method thereof |
| CN104087633A (en) * | 2014-07-04 | 2014-10-08 | 西藏圣龙实业有限公司 | Method for improving content of polysaccharides in highland barley based on solid fermentation |
| CN105725182A (en) * | 2016-02-01 | 2016-07-06 | 中国人民解放军总后勤部军需装备研究所 | New application of schizophyllum commune fermentation broth |
| CN107308073A (en) * | 2017-06-26 | 2017-11-03 | 上海应用技术大学 | A kind of highland barley fermentation magma and preparation method and application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114081862A (en) * | 2020-07-31 | 2022-02-25 | 伽蓝（集团）股份有限公司 | Preparation and application of saccharomyces cerevisiae fermentation product from highland barley wine yeast in pan-Himalaya region |
| CN114081862B (en) * | 2020-07-31 | 2023-06-16 | 伽蓝（集团）股份有限公司 | Preparation and application of Saccharomyces cerevisiae fermentation product from highland barley wine distiller's yeast in Pan-Himalaya region |
| CN115191551A (en) * | 2022-07-20 | 2022-10-18 | 四川省雅士科技有限公司 | Anoxia-resistant fermentation broth, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106190699B (en) | A kind of preparation method of brown rice wine of rice fermented with red yeast | |
| CN105831695B (en) | A kind of preparation method of low-salt health-care soy sauce | |
| CN108260808B (en) | Noni enzyme and preparation method thereof | |
| CN101423856B (en) | Aminoacid polypeptide nutrient fluid, preparation method thereof and use | |
| CN105969636B (en) | A kind of preparation method of brown rice monascus vinegar | |
| CN109717340A (en) | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis | |
| WO2009074004A1 (en) | A method for preparation of a health care wine of cordyceps | |
| CN102885303B (en) | High-r-aminobutyric-acid-content highland-barley red yeast and preparation method thereof | |
| CN107488598B (en) | Burdock-based cordyceps militaris mycelium and preparation method thereof | |
| CN103190576B (en) | Cordyceps militaris soybean and preparation method thereof | |
| CN103865747A (en) | Production method of monascus vinegar rich in lovastatin | |
| CN1759760A (en) | Method for producing fruit juice of germinant unpolished rice and ganoderma lucidum, and production | |
| CN109691670A (en) | A kind of preparation method and applications of dendrobium candidum enzyme liquid | |
| CN102351580B (en) | Method for preparing black fungus nutrient solution by utilizing food processing residues | |
| CN102835624A (en) | Ganoderan noodle and production method thereof | |
| CN108771116A (en) | A kind of processing method of multi-functional composite rice | |
| CN109200214A (en) | A kind of highland barley glossy ganoderma fermentation magma and preparation method thereof | |
| CN110616122A (en) | Cardamine hupingshanensis selenium-rich yellow wine and production method thereof | |
| CN102577918B (en) | Method for preparing ganoderma lucidum mycelia by aid of germinated brown rice | |
| CN103229666B (en) | Cordyceps militaris peanut and preparing method thereof | |
| CN102911977A (en) | Method for producing monacolin k by fermenting monascus with solid matrix served as carrier | |
| CN110129163A (en) | A method of producing hickory chick yellow rice wine | |
| CN105713786A (en) | Preparation method of healthy kudzuvine root liquor | |
| KR100844980B1 (en) | Method of producing a Healthy Mulberry Leaf Food by culture of Mushroom Mycelia | |
| CN103719744A (en) | Preparation method of Monacolin K-rich lobster sauce |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |
|
| RJ01 | Rejection of invention patent application after publication |