CN1259569A

CN1259569A - Biotechnology cultivation and processing of lucid ganoderma extract

Info

Publication number: CN1259569A
Application number: CN 98124982
Authority: CN
Inventors: 张雪岳
Original assignee: Individual
Current assignee: Individual
Priority date: 1998-11-27
Filing date: 1998-11-27
Publication date: 2000-07-12

Abstract

Use cultured bacterial strain to fermant and culture into liquid bacterial spawn, increase inoculum (15-20%) and inoculated on wood dust culture medium which is best formulated for glossy genoderma culture medium and is put into small bag, then ferment and culture to ripe, when the content is highest yet not converted to leathery cell wall, extract more than 90% effective ingredient, vacuum concentrate under reduced pressure (below 60 deg.c) to obtain glossy genoderma extract product which retains nearly all the effective ingredient which is easy to be absorbed by human body. It solves the problem of breaking the leathery cell wall of glossy genoderma.

Description

Biotechnology cultivation and processing of lucid ganoderma extract

What the present invention relates to is the method for cultivating Ganderma lucidum and processing of lucid ganoderma extract with biotechnology.

That has found in the macro fungi of Ganoderma Ganodenna has tens of kinds, is that red sesame, red sesame are representative species with glossy ganoderma Ganoderma bucidum wherein.Glossy ganoderma can make the people come back to life, legend, the mythology of treatment all kinds of diseases and ailments, no scientific basis.The high surprising ganoderma lucidum prod of propaganda that some are excessively made an overstatement and with low quality and valency has caused confusion, makes the true effect of glossy ganoderma also be subjected to people's suspection, and also the true colours of glossy ganoderma have been given play to its due effect.Materials such as ganoderan, amino acid, alkaloid, triterpenes, coumarin glucoside, trace element, VITAMIN, enzyme, be the good healthcare products of a class, also can prevent and treat multiple disease, the micro-organic germanium in the glossy ganoderma can strengthen the new vigor of blood of human body and oxygen, strengthens metabolism.Ganoderan, polypeptide, triterpenes, enzyme, organic germanium etc. can strengthen the immunizing power of cell, and tumoricidal not dead end granzyme stops the synthetic of cancer cells gene DNA, RNA, plays the effect of cancer preventing and treating, and has no side effect.The effective ingredient of glossy ganoderma is the effect uniqueness aspect adjusting human viscera organ function, raising immunizing power, anti-ageing, anti-curing oncoma.Because the widespread use of medical science, wild Ganoderma and finishing technology can not satisfy the demands far away, proposed new problem for like this cultivation and the processing technology of glossy ganoderma, though cultivate Ganoderma sporophore through the beginning of the seventies at the end of the sixties, and extract effective ingredient with spore powder and make " Ganoderma Syrupus ", " Ganoderma granule ", created the cultivation technology of " bottle is planted glossy ganoderma " same period.Though in later more than ten years we in the research of glossy ganoderma some the progress, do not have big breakthrough, exist the bacterial strain growth period long, pollution rate is high; Wildly contain composition because of the place of production is different with basic thing and differ greatly with cultivation; Yield poorly, effective constituent is few; The growth cycle is long; Precision work efficient is low, quality is low, cost is high.To the intermediary and later stages nineties, on the basis of research many decades mushroom, breed the new rapidly bacterial strain of a kind of red sesame stable high yield growth, prepare the best glossy ganoderma culture material prescription of successfully a kind of stable high yield, with biotechnology fermentation culture glossy ganoderma and extract 90% above effective ingredient, solved a difficult problem that is difficult to brokenly the keratin cell walls.

Purpose of the present invention provides a kind of red sesame stable high yield bacterial strain rapidly of growing of breeding, and prepares the best glossy ganoderma culture material prescription of a kind of stable high yield and with the method for biotechnology fermentation culture glossy ganoderma and processing of lucid ganoderma extract.

The objective of the invention is to realize through following approach:

Sporophore separation and Culture with red sesame becomes mycelium, handles and experiment in cultivation through four ultraviolet mutagenesises, selects the metastable bacterial strain that is fit to requirement; With the Fagaceae wood chip is the main best glossy ganoderma culture material prescription that is mixed with; Turn out glossy ganoderma with biofermentation method, carry out retrofit and become the glossy ganoderma essence.

The glossy ganoderma of adopting such scheme to cultivate can extract 90% above effective ingredient, has solved a difficult problem that is difficult to brokenly the keratin cell walls.Glossy ganoderma essence and the preparation thereof produced are evident in efficacy, and reduce production costs greatly, in the cultivation of glossy ganoderma and processing breakthrough have been arranged.

Below further its concrete batching and working method of narration:

One, material and method:

1. strain selection: the sporophore separation and Culture with red sesame becomes mycelium, divides some groups, on clean work station, use different time respectively with ultraviolet ray, the different distance irradiation, select the culture dish that kills mycelium 98%, the dilution tube is cultivated into strains for cultivation, select wherein that growth is fast, a shape is good, the bacterium of strong resistance bag, from sporophore, isolate bacterial classification again, again with ultraviolet ray suitable distance and time irradiation, the mycelium tube of mutagenesis is cultivated into strains for cultivation, select the sporophore separation and Culture that is fit to require again and become bacterial classification.Handle and experiment in cultivation through four ultraviolet mutagenesises like this, select the metastable bacterial strain that is fit to requirement.

2. prepare burden: Fagaceae wood chip 75%, cornstalk chaff 10%, wheat skin 10%, sucrose 1%, calcium superphosphate 2%, terra alba (calcium sulfate) 2%, water content 50% (will insert 15～20% liquid spawn during inoculation), transfer pH value to 6.5～7 with lime powder.

3. fermentating culturing process flow process:

The female kind of glossy ganoderma separation and Culture → constant-temperature shaking culture liquid original seed → submerged fermentation cultivation liquid spawn → batching pack → autoclaving → access liquid spawn → send out a bacterium to cultivate → manage → gather → drying.

4. glossy ganoderma fine-processing technique flow process:

Leaching juice → cross bacterium slag charge face with water logging to boil and crossed leaching juice → four juice in 1 hour and merge the recovery part solvent was crossed in the solvent lixiviate that leaching juice → with 50% was crossed in the solvent lixiviate that leaching juice → with 70% was crossed in the nonpoisonous organic solvent lixiviate of raw material stoving (it is the abundantest and gather when also not forming the keratin cell walls to nutritive ingredient to send out bacterium) → with 95% in 24 hours in 24 hours in 24 hours, and be concentrated into Baume 33 with vacuum decompression (below 60 ℃) and spend → in ratio adding Zulkovsky starch absorption → cryodrying → pulverizing (ratio of raw material and extraction glossy ganoderma essence 5: 1) of 1: 1.

Two, result:

1. the bacterial strain bred of mutagenesis covered with the bacterium bag in 7～10 days in advance than original strain, polluted fewly, found in the experiment to pollute, and the original strain pollution rate reaches about 20%.

2. the best glossy ganoderma culture material prescription of preparation is the Fagaceae wood chip that grows based on the optimum glossy ganoderma, experiment showed, the wild problem that differs greatly because of the place of production, the different content compositions of basic thing with cultivating ganoderma that solved.

3, output and the high glossy ganoderma of effective ingredient have been turned out with fermentation engineering bacterial strain, best culture material prescription, contain pure mycelium 26%, total reducing sugar 8.35%, polysaccharide 4.70%, protein 33.5%, alkaloid 23.4%, 16 kinds of trace elements, 18 seed amino acids, and the Ganoderma spore powder of contrast, polysaccharide content is 0.38%, protein 16.06%, alkaloid 4.69%.Above-mentioned analytical results shows that effective constituent is than more than ten times of the high several times to two of spore powder.

4. send out that bacterium is cultured to maturation and during the also unconverted formation keratin cell walls of partial protein and sugar, various effective constituents are the highest, extracting effective components also is easier to.We propose the glossy ganoderma essence with 5: 1 ratio, and nearly all effective constituent can both preserve, and is not damaged, and is easy to the absorption of human body utilization.

5. cultivate the glossy ganoderma essence of processing with biotechnology, new bacterial strain, optimum proportion, advanced technology not only, output and quality height, production cost are also low, than the low several times of commercially available ganoderma lucidum prod, tens of times to more than hundred times.

6. the conventional glossy ganoderma of cultivating is taken 30 to 40 days under optimum conditions and just can make mycelium cover with the bacterium bag, and this differentiates the keratin cell walls with regard to the part mycelium that a growth earlier occurs is long to physiological maturity, and then Chang mycelium does not also cover with the bacterium bag.Our growth speed is fast, and liquid spawn access amount is increased to 15%～20%, makes mycelium begin to send out bacteria growing everywhere simultaneously in the bacterium bag.Not only use best batching, also make the bacterium bag into pouch, make mycelium cover with bag as early as possible.Therefore, can shift to an earlier date 10～15 days and send out well bacterium, and be easy to extract the effective ingredient more than 90%, this also can be described as a breakthrough on the processing technology.

Claims

1. biotechnology cultivation and processing of lucid ganoderma extract is characterized in that: with the Fagaceae wood chip is the main best glossy ganoderma culture material prescription that is mixed with:

Fagaceae wood chip 75%, cornstalk chaff 10%, wheat skin 10%, sucrose 1%, calcium superphosphate 2%, terra alba (calcium sulfate) 2%, water content 50% (will insert 15～20% liquid spawn during inoculation) are transferred pH value to 6.5～7 with lime powder.

2. biotechnology cultivation and processing of lucid ganoderma extract is characterized in that: an advanced person's cultivation glossy ganoderma technical process is arranged:

Female kind → constant-temperature shaking culture the liquid of strains separation cultivation original seed → submerged fermentation cultivation liquid spawn → batching dress pouch → autoclaving → access liquid spawn (15～20%) → send out a bacterium to cultivate → manage → gather → drying.

3. biotechnology processing of lucid ganoderma extract is characterized in that: a glossy ganoderma fine-processing technique flow process is arranged:

Leaching juice → cross bacterium slag charge face with water logging to boil and crossed leaching juice → four juice in 1 hour and merge the recovery part solvent was crossed in the solvent lixiviate that leaching juice → with 50% was crossed in the solvent lixiviate that leaching juice → with 70% was crossed in the nonpoisonous organic solvent lixiviate of raw material stoving (it is the abundantest and gather when also not forming the keratin cell walls to nutritive ingredient to send out bacterium) → with 95% in 24 hours in 24 hours in 24 hours, and was concentrated into ratio adding Zulkovsky starch absorption → cryodrying → pulverizing (ratio of raw material and extraction glossy ganoderma essence 5: 1) that 1: 1 is spent → connect to Baume 33 with vacuum decompression (below 60 ℃).