CN1252997A - Process for preparing hemopeptidin and hemopeptidin products - Google Patents
Process for preparing hemopeptidin and hemopeptidin products Download PDFInfo
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Abstract
A hemopeptidin is prepared from edible animal blood through special process and biodegradation technique to destroy the structure of blood protein and decompose the protein into short peptide and amino acids. The raw hemopeptidin powder contains, polypeptide, amino acids and heme iron, and can be used to relax iron-deficiency anemia. It can also be used as additive for food or medicine to prepare health-care food or medicine for supplementing iron to human body.
Description
Peptide native is through BIOLOGICAL CONTROL transformation technology and specific prepared a kind of heme iron and hemoglobin degrading polypeptide and the amino acid whose biological product of being rich in of technology with animal blood.Relate to biological enzymolysis technology, contain peptide food and medicinal preparation technical field.
Animal blood is a kind of precious resources, has very high healthy nutritive value, and human nutrition is had very important meaning.In recent years, countries in the world all take much count of utilizing the research and the production of goods such as animal blood conducting food additive, health product and medicine, and the exploitation of animal blood becomes the emerging field that the utmost point has development potentiality.Chinese the meat production amount is in world-class position always since 1993, and live pig output is near 1/2nd of world's total amount, and China produces 1,800,000 tons animal blood every year at present, but major part does not all obtain utilizing serious waste of resources effectively fully.
China just pays much attention to the nutrition health-care functions of blood since ancient times.The traditional Chinese medical science thinks that blood is the source of human body vital essence, and insufficiency of blood can cause comprising that other organ pipe of brain is impaired.The method of enriching blood is varied, wherein, edible animal blood can be mended the deficiency of human body vim and vigour, so " the blood bean curd " made with poultry blood plasma is a kind of tonic product that people like always, but this blood products that forms through the direct heat processing by former blood plasma, because cellularity and macro-molecular protein are without effective destruction, it is extremely low that it absorbs metabolic rate, causes the significant wastage of nutrition.
Modern nutriology thinks that heme iron is a kind of important nutrient that plays blood tonification effect in the blood products.Haemachrome is made of porphyrin ring and ferrous iron, so heme iron is called ferrous porphyrin or organic ferrum again, the content of ferrum is up to more than 40 milligrams in per 100 gram blood, and lean beef, thin Carnis caprae seu ovis and thin pork then have only 2.6,2.0 and 1.6 milligrams.Human body is three times of inorganic iron to the absorbance of ferrous porphyrin, and is not subjected to the influence of vitamin C or iron chelating agent, so, the ferrous porphyrin in the blood for improving because the iron deficiency anemia effect that iron deficiency or malabsorption iron deficiency are caused in the food is obvious,
Ferrous porphyrin has curative effect to iron deficiency anemia and is proved.Prior art all is the extracting method about haemachrome, and the compound method of haemachrome capsule, heame anti-anemina medicine thing.As: 92102700.1 " extracting method of haemachrome " disclose the method for purifying with anticoagulant (trisodium citrate) blood sampling, antioxidant (sodium sulfite) hydrolysis, (sodium hydroxide) alkalization, (sodium acetate) precipitation, (ethanol); 94100816.9 " haemachrome capsule and preparation technology thereof " discloses the method for haemachrome dry powder+Radix Astragali+Radix Polygoni Multiflori Preparata+Pericarpium Citri Reticulatae; 9810767429 " heame anti-anemina medicine and preparation methoies ", disclosed is to be the main solution that extracts with acidic ethanol, make haemachrome and ethanol generation esterification in the blood, in alkaline solution esterification catalysis is become the haemachrome sodium salt then, reuse acid is separated out filtration with the haemachrome precipitation.
Thus, produced aforesaid and noted the effect of haemachrome in the blood, and ignored the effect of protein and other nutrient substance in the blood, caused the waste of resource, it is low to absorb metabolic rate, the unconspicuous shortcoming of medical health effect.
Through Centre for Social Science Information and Documentation of the Chinese Academy of Sciences the present invention is carried out domestic and foreign literature retrieval (21 pieces of domestic literatures, 15 pieces of foreign literatures), the following new interpretation of result of looking into has been proposed: ... this problem adopts the closed blood collecting technology, collect the edibility animal blood, with physical process separating treatment blood and carry out the multistage conversion of enzyme process, product is the major function sexual element with polypeptide, aminoacid and heme iron.Domestic and foreign literature is not seen and the similar research report of these problem characteristics.(See Attachment 1: " the Application and Development research of peptide native " domestic and foreign literature search report in full).
The object of the invention is to make full use of edibility animal blood resource, and Simple and Reliable blood treatment biodegradation process is provided, thereby preparation is the peptide native finished product of major function sexual element with polypeptide, aminoacid and heme iron.
The present invention also aims to the peptide native is health care or medicinal raw material, and preparation has the iron supplement effect, improves the health food or the medicinal preparation of symptoms such as auxotype anemia, QI and blood deficiency.
The objective of the invention is to be achieved through the following technical solutions:
With pig, cattle, mammals such as sheep, or chicken, birdss such as duck, or Trionyx sinensis Wiegmann, Serpentis, the edibility blood of economic animals such as deer is raw material, technical process through the blood collecting-former powder of DC separation-one-level conversion-secondary conversion-peptide native-preparation-product, adopt biological enzymolysis technology, destroy the protein macromolecule structure, decomposing protein is small peptide and aminoacid, preparation is with polypeptide, aminoacid and heme iron are the former powder of the peptide native of Main Ingredients and Appearance, and the former powder of this peptide native has and improves because the effect of the human body iron deficiency anemia that iron deficiency or malabsorption iron deficiency are caused in the food.Be additive with the former powder of peptide native again, prepare health food or the medicinal preparation that has iron supplement, improves symptoms such as auxotype anemia, QI and blood deficiency with the food or the medicine of different shape.
It is to adopt the closed blood-sampling method that animal blood is collected, promptly adopt hollow blood sampling knife to connect the sealing mode of flexible pipe and blood sampling bucket successively, collect 50 liters of blood bucket dischargeable capacitys, load-bearing is an optimum for 40 kilograms, blood sampling time was controlled between 10～40 seconds, and collection process and conveyer chain keep carrying out synchronously.Do not add anticoagulant in the blood collection procedure, or only add a small amount of anticoagulant.
Blood collecting is to carry out at normal temperatures, collect the back and within 4 hours, carry out DC separation pretreatment, stirring, broken and homogenizing blood cell structure, form the haemolysis state of three layer components, spray into a small amount of enzyme liquid and eliminate upper foam, with fibrin, cell debris and the lipid in 40 order net skim middle levels.The blood plasma of lower floor and cell leachable can directly become pending blood plasma.
Aforesaid anticoagulant is 5～40% sodium citrate aqueous solution, and consumption is to add 1～10 milliliter of anticoagulant in per 100 milliliters of blood.
DC separates pretreated animal blood, and promptly pending blood is through two-stage biological degradation technique conversion process.
One-level transforms, be in bioreactor slowly under the stirring condition, DC is separated pre-treated animal blood be heated to 40-70 ℃, after treating temperature stabilization, slowly add endopeptidase Alcalase2.4L, addition is the 0.1-2.0% of blood plasma volume, kept thermotonus 0.5-6 hour, control DH value 5-15% makes hemocyte all dissolvings gradually, the pH of blood plasma gradually when the 7.5-7.6 of former blood drops to 5.8-6.5 only.
Under the equal physical condition that transforms for the first time, to cellularity disappearance, the blood plasma that macro-molecular protein is decomposed into small peptide carries out second level conversion.Promptly add weakly acidic exopeptidase Flavourzyme1000L and small peptide is done further to repair release, addition is the 0.1-2.0% of blood plasma volume, continues reaction 0.5-6 hour.The DH value is controlled at 30-70%.
In the two-stage conversion process, all be to measure and control DH value (degree of hydrolysis), (h mmol/g) accounts for total peptide bond mM number (h promptly to measure the mM number of peptide bond cleaved in every gram material protein
Tot, percent value mmol/g) is determined with enzyme amount, response time and reaction temperature.Its calculating formula is:
DH＝h/h
tot×100％。
In the two-stage conversion process, the enzyme preparation that is adopted is to meet the papain that Alcalase endopeptidase, Neutrase endopeptidase, Protamex endopeptidase+exopeptidase, Flavourzyme1000 exopeptidase, the Guangzhou enzyme preparation factory of the NOVO company that food stage requires produce.
The secondary conversion process will be carried out enzyme denaturing to goods after finishing.Be 70～90 ℃ of temperature, keep carrying out under the condition in 10-40 minute.
Peptide native oleo stock in the preparation process should adopt spray drying method.Inlet temperature 180-220 ℃, leaving air temp 70-80 ℃, and also material should keep stirring in process to be dried.
Peptide native oleo stock can also adopt the freeze-drying dehydration, preparation peptide native finished product.
The peptide native final finished is Powdered, bronzing, little light bitterness, free from extraneous odour, moisture content≤10%, protein 〉=60%, ferrum 〉=2000mg/kg.
The peptide native finished product can by every day 2 gram dosage directly as symptoms such as iron deficiency anemia, QI and blood deficiency tonic.
The peptide native finished product can also can be used as the additive of solid-state food, liquid beverage, paste food.
All right and the Cordyceps of peptide native finished product, relevant Chinese medicinal components compatibilities such as the Radix Astragali, Radix Codonopsis, Fructus Lycii, Colla Corii Asini prepare the peptide native medicinal preparation or the health food of agent in many ways.
The peptide native finished product be with above-mentioned Chinese medicinal components in any one, any two kinds, any three kinds, any four kinds of formulated in combination.
Above-mentioned peptide native medicinal preparation or health food are to prepare in proportion with the powder or the extract of relevant Chinese medicinal components.
The main form of peptide native medicinal preparation or health food is the peptide native coated tablet, peptide native electuary, peptide native capsule, peptide native oral liquid.
By above technical scheme and measure, make the present invention explain the notion that the animal blood edibility is utilized first, in conjunction with practical situation exploitation animal's whole blood, adopt BIOLOGICAL CONTROL transformation technology and processing technique, preparing the peptide native product that is rich in the trophic factors such as organic ferrum, hemoglobin degrading polypeptide and aminoacid that are easy to absorption of human body, is a kind of peptide native preparation method and product of high technology content high added value.Through animal experiment and clinical proof, this product all has the medical care effect that prevention improves to symptoms such as iron deficiency anemia and nutritional anemia, QI and blood deficiency.
The Henan Province college of traditional Chinese medicine has carried out at the experiment management center zoopery of " peptide native is to the influence of the bad model of blood dificiency of rat hemorrhagic anemia Ensure Liquid (iron deficiency anemia) ".Experimental result shows: the peptide native preparation has certain improvement and mitigation to rat iron deficiency anemia model, can significantly improve the amount and the erythrocyte number (P＜0.05) of model group rat hemoglobin in this experiment.(seeing Appendix two)
The clinical observation result of the peptide native treatment iron deficiency anemia infant that carries out through Henan Province's Shangqiu City First People's Hospital proves: iron deficiency sexually transmitted disease (STD) infant, after taking peptide native treatment course of treatment, Hb, MCV have the utmost point to significantly improve (P＜0.01), RBC, Ret be significantly increased (P＜0.05), do not improve though MCH, MCHC have obviously, before taking certain rising is arranged also.
The present invention proposes the simple and feasible closed blood collecting method of a cover, adopt unique DC separation process pretreatment blood, can destroy the organizational structure of blood effectively, and equipment is simple, the efficient height, cost is low.
The peptide native finished product can be widely used in food additive or medicinal preparation except that direct use, thereby, it of many uses, product category is many.
Above-mentioned characteristics are enough to explanation and obtain the technical scheme support and realized purpose of the present invention.
Below in conjunction with the embodiment explanation more detailed in addition to the present invention:
Indication animal of the present invention, mammals such as the pig that refers generally to raise, cattle, sheep also can be birdss such as chicken, duck, in particular cases, can also be extraordinary economic class animals such as Trionyx sinensis Wiegmann, Serpentis, deer, and must be the healthy animal by quarantine.Following process prescription all is example with the pig; Indication animal blood of the present invention refer in particular to the scale meat producing plant in slaughtering process, adopt certain means collected meet the clean animal blood that can be used for further consumption processing that food hygiene requires, following process prescription all is example with the Sanguis sus domestica.
The closed blood collecting technology is adopted in the collection of animal blood of the present invention.Collection process can be carried out at normal temperatures, blood collecting can not worry blood coagulation and haemolysis.The blood of collecting if can not further handle, should be considered stored refrigerated in 4 hours.
Blood clean is collected and can be divided into small lot blood sampling and two kinds of processes of scale blood sampling, all adopt hollow thorn blood cutter, can not add anticoagulant in the blood, or only add a small amount of anticoagulant, the purpose that adds anticoagulant is that to prevent that blood from irreversibility taking place in put procedure clean with fixed attention.Anticoagulant is the sodium citrate aqueous solution of 5-40%, and consumption is that per 100 milliliters of blood add 1-10 milliliter anticoagulant.
In small lot when blood sampling,, hollow knife is terminal to be pricked collection blood bag and cutter hub can be fixed on the trunk with hook, and collection blood bag must be made and has certain intensity with the material that meets the food hygiene requirement, and capacity is the 2-5 liter.The live body qualified live pig of quarantining, reversal of the natural order of things after shocking by electricity, hollow knife thrusts in the neck place gets blood, and the thorn blood time is controlled at 10-40 second, and the blood sampling volume of every pig is the 1.5-3.5 kilogram.Used hollow knife cleans the back and reuses after plucking collection blood bag.Place separately the blood numbering back of collecting, treat that the quarantine of corresponding trunk and internal organs is qualified after, it is standby that qualified blood is incorporated in storage blood jar.
During the scale blood sampling, the hollow knife end links to each other with collection blood bucket with flexible pipe.Flexible pipe must be made and inner wall smooth with the material that meets the food hygiene requirement, and internal diameter should be enough greatly to guarantee blood flow and to reduce agglomerative probability in the blood tube.Collection blood bucket is made with rustless steel, and dischargeable capacity is 50 liters, and top cover can rotate, and three to five adapters are arranged above, and each adapter can connect a flexible pipe.20 to 30 seconds thorn blood time on slaughter line.The blood of per 20 pigs is 1 barrel, is about 40 kilograms, if this batch trunk and internal organs quarantine are qualified, this barrel blood can be used for peptide native production.Defective if quarantine, blood can be done non-consumption and handle.
Peptide native small lot test technology
| Batch | Fresh blood | Pretreatment | Spray drying | Total recovery % | ||||
| Weight kg | Content % | Weight kg | Loss % | Weight Kg | Moisture content % | Loss % | ||
| ????1 | ??3.80 | ??18.2 | ??3.62 | ??????4.7 | ??0.645 | ??2.70 | ???4.9 | ??90.6 |
| ????2 | ??3.50 | ??18.8 | ??3.30 | ??????5.7 | ??0.610 | ??2.86 | ???4.2 | ??90.3 |
| ????3 | ??4.00 | ??18.6 | ??3.67 | ??????8.2 | ??0.682 | ??5.65 | ???5.4 | ??86.8 |
| ????4 | ??3.50 | ??18.2 | ??3.32 | ??????5.1 | ??0.590 | ??4.44 | ???6.0 | ??89.2 |
| ????5 | ??3.50 | ??18.5 | ??3.24 | ??????7.4 | ??0.598 | ??6.34 | ???6.6 | ??86.5 |
| On average | ??????6.22 | ???5.4 | ??88.7 | |||||
According to technological process and the processing parameter established, build up the pilot production line that class handles 300 kilograms of animal bloods in industrial microorganism institute biological engineering pilot scale base, Tianjin.Production line is made up of freezer, agitator tank, homogenizer, Cooling or heating jar, spray drying tower, drying room etc., and armamentarium comprises that delivery pump and connecting pipe all adopt stainless steel, and workshop is built by the food production hygienic requirements.
Pilot production line is handled 310 kilograms, 320 kilograms and 320 kilograms of Sanguis sus domestica respectively through three trial productions.Experiment showed, that this Design of Production Line is reasonable, meet technological requirement, the disposal ability of equipment reaches per tour more than 300 kilograms.
The yield of producing as a trial in the peptide native
| Batch | Fresh blood | Spray drying | Total recovery % | ||
| Weight kg | Content % | Weight Kg | Moisture content % | ||
| ?1 | ???310 | ????17.8 | ????48.4 | ????5.6 | ????82.8 |
| ?2 | ???320 | ????18.0 | ????51.7 | ????4.8 | ????85.5 |
| ?3 | ???320 | ????17.6 | ????49.1 | ????4.5 | ????83.3 |
| On average | ????83.8 | ||||
The present invention carries out DC separation process pretreatment to collected clean animal blood, and purpose is in the short period of time, destroys the coagulability and the hemocyte structure of blood plasma, obtains the homogeneity blood plasma of good fluidity, is convenient to zymolysis.
Utilize fibrin in the blood to erythrocytic combination, Lock-in is partly condensed, and broken and homogenizing is handled and destroyed cellularity with agitator again, produces haemolysis.Blood through processing leave standstill after very fast formation three layer components, the upper strata is a froth bed, is the hematoglobin protein formation of bubbling when vigorous stirring, sprays into that a small amount of enzyme liquid is very fast to be understood froth breaking and become blood plasma; The middle level is heavy-gravity fibrous material, by coagulating the fibrin that and crosslinked cell debris and a small amount of lipid material formed, with 40 order copper mesh skim fully; The red slurry that lower floor is made up of blood plasma and cell leachable and small amounts of cells presents good flowability, natural coagulation no longer occurs and produces undesirable abnormal smells from the patient in the processing procedure afterwards or during cold preservation.This process is called the DC separation process, and its feature is not need to add anticoagulant or only add a small amount of anticoagulant to achieve the goal, and technology is simple, is suitable for the industrialization operation, and can effectively keep the quality and the natural sex of final products.
The present invention carries out under temperate condition in bioreactor the biotechnology conversion process of animal blood.Bioreactor can be a stainless steel, also can be enamel or glass material, and inner wall smooth is easy to clean; Agitating function and indirect and refrigerating function are arranged, and automatic control or manual control can keep temperature required stablizing in course of reaction.
The present invention adopts two-stage to transform to the biotechnology conversion process of animal blood, carries out according to the following steps:
Separate pre-treated animal blood through DC, in bioreactor, under slow stirring condition, be heated to 40-70 ℃.Behind the temperature stabilization, slowly add endopeptidase Alcalase2.4L and carry out first order conversion, addition is the 0.1-2.0% of former blood plasma volume, keeps thermotonus 0.5-6 hour.In course of reaction, increase with the DH value, hemocyte is all dissolvings gradually, and the pH of blood plasma drops to 5.8-6.5 from the 7.5-7.6 of former blood gradually.
In the course of reaction, measure DH value (degree of hydrolysis is meant that the mM number of peptide bond cleaved in every gram material protein accounts for the percent value of total peptide bond mM number) and control with enzyme amount and response time and reaction temperature.
After the first order transformed and finishes, cellularity disappeared, and macro-molecular protein is decomposed into small peptide, and the pH of converted product decreases, and the DH value of blood plasma can reach 5-15%.
Keep said temperature, add weakly acidic exopeptidase Flavourzyme and small peptide is done further to repair to release carry out secondary and transform, addition is the 0.1-2.0% of former blood plasma volume, continues reaction 0.5-6 hour.After the second level transformed and finishes, the protein small peptide is further decomposed produced aminoacid, and the DH value rises rapidly, can reach 30-70%.
The protease preparation of the used biological preparation of the present invention for from microbial fermentation product or plant, extracting, can decompose hematoglobin protein under specific temperate condition becomes micromolecule oligopeptide and aminoacid, and used enzyme preparation all meets the food processing requirement.Realize that best enzyme preparation of the present invention is Alcalase2.4L (food stage, NovoNordisk company product, liquid) and Flavourzyme1000L (food stage, Novo Nordisk company product, liquid).The protease of other type, as: Neutrase2.4L (Novo Nordisk company product, liquid), Protamex1.5L (Novo Nordisk company product, liquid) and papain (enzyme preparation factory in Guangzhou produces, solid) also can be used to reach this purpose.
The main purpose that hematoglobin protein is transformed is that decomposing protein becomes small peptide and aminoacid, promotes the absorption of nutrient substance and helps the absorption metabolism of heme iron, destroys proteinic macromolecular structure simultaneously, improves its dissolubility.
After the secondary conversion process finishes, be heated to 70-90 ℃ and keep 10-40 minute enzyme denaturing, in heating enzyme denaturing process, the DH value still has the increase of 5-10% usually.
Above-mentioned product of the present invention promptly is a peptide native oleo stock, has good flowability, can produce the brown flocculent deposit after leaving standstill, and slowly can not precipitate under the stirring.
Above-mentioned peptide native oleo stock is rich in the thermal sensitivity organic substance, and suitable employing can instantaneous exsiccant spray drying method.Material should keep stirring in process to be dried.The control inlet temperature is 180-220 ℃ in the dry run, and leaving air temp 70-80 ℃, temperature is lower than 70 ℃ of products and easily lumps.The former powder of gained peptide native is the product of brick-red good fluidity, water content 3-8%, and also solubility is very good.
Above-mentioned peptide native oleo stock can also adopt the freeze-drying dehydration, can obtain even more ideal peptide native product, but can cause the rising significantly of cost usually.
The nutrient analysis of peptide native product
The peptide native product detects through Chinese food quality supervision and test center, contains whole 18 seed amino acids, and amino acid whose total amount wherein must account for 54% of total amount by amino acid whose content up to 87%; The content of ferrum element is per kilogram 2124 micrograms, also contains trace element such as a certain amount of copper, zinc, calcium in addition.Providing in the peptide native must amount of amino acid and the content of human milk, shell egg and theoretical reference value.The heavy metal index and the microbiological indicator of peptide native product all meet food hygienic standard.
The milligram ordered series of numbers following table of the contained essential amino acids of every gram protein:
Annotate: theoretical pattern refers to satisfy the amino acid pattern of the high quality protein of body needs, according to baby and 10-12 the year child needed essential amino acids design.(source of data: work such as A.H. Ensminger; " nutrient ")
| Aminoacid | The tyrosine phenylalanine | Leucine | Lysine | Valine | Isoleucine | Threonine | The methionine cystine | Histidine | Amount to |
| Theoretical pattern | ????73 | ??70 | ???51 | ??48 | ??42 | ????35 | ????26 | ????17 | ???373 |
| Human milk | ????99 | ??95 | ???68 | ??63 | ??56 | ????46 | ????40 | ????23 | ???507 |
| Shell egg | ????98 | ??88 | ???68 | ??72 | ??63 | ????49 | ????56 | ????24 | ???534 |
| Peptide native | ????80.8 | ??119.7 | ???76.1 | ??74.1 | ??13.3 | ????31.1 | ????17.3 | ????59.8 | ???472.2 |
| Chemistry divides | ????82 | ??136 | ???112 | ??103 | ??21 | ????63 | ????31 | ????249 |
Chemistry divides, and shell egg is as reference protein matter.
About the peptide native product:
In order to verify the effect of peptide native, entrust pathology chamber, Henan College Of Traditional Chinese Medicine experiment management center to do zoopery with the peptide native powder respectively, observe its influence to the bad model of blood dificiency of rat hemorrhagic anemia Ensure Liquid (iron deficiency anemia), offer Shangqiu City First People's Hospital, Henan Province department of pediatrics with the peptide native capsule, the iron deficiency anemia infant is carried out oral medication (seeing laboratory report).
Normal adult every day, the requirement to the ferrum element was the 1-2 milligram, and the tonic that adopts is inorganic source of iron usually, and its absorbance only is 10%, was the 15-20 milligram so the threpsology goes up chalybeate intake every day of recommendation.The absorbance of the contained ferrous porphyrin of peptide native is up to 30%, and the content in every gram product is greater than 2 milligrams, and contained polypeptide and aminoacid can satisfy the needs of human body to the ferrum element for the facilitation preferably that is absorbed with of ferrum element so take peptide native 2 grams every day.
The clinical observation object of peptide native effect is the light MIDA infant of outpatient service, and the peptide native that is provided is that capsule (0.25 gram/grain) and press mold become plate (10/sheet).The original plan dosage is eight of every days, is equivalent to the amount of peptide native 2 gram, and the morning, noon and afternoon take for twice, with fortnight be a course of treatment.Dosage during actual carrying out is kept to 6 of oral peptide native capsules every day, is a course of treatment with two weeks, forbids methods treatments such as other chalybeate or blood transfusion during this period.Clinical observation result shows: iron deficiency sexually transmitted disease (STD) infant, after taking peptide native treatment course of treatment, Hb, MCV have the utmost point to significantly improve (P＜0.01), RBC, Ret be significantly increased (P＜0.05), do not improve though MCH, MCHC have obviously, before taking certain rising is arranged also.Illustrate that peptide native has curative effect preferably to light MIDA infant, any toxic and side effects do not occur in the application process.
Peptide native zoopery dosage is set according to interrelated data, divide two dosage 0.2g/kg and 33mg/kg to rat, administration every day 1 time, successive administration 14 days, make comparisons with Feosol simultaneously, observe its influence the bad model of blood dificiency of rat hemorrhagic anemia Ensure Liquid (iron deficiency anemia).Experimental result shows: the peptide native preparation has certain improvement and mitigation to rat iron deficiency anemia model, can significantly improve model group rat hemoglobin amount and erythrocyte number in experiment; The peptide native preparation is compared with inorganic iron-supplementing preparation ferrous sulfate commonly used, more can effectively improve model group rat hemoglobin level, but similar to increasing the aspect effect of model group rat erythrocyte number.
So just can determine that the peptide native finished product can be directly as tonic, day dose 2 grams can satisfy the needs of human body ferrum element.
The peptide native finished product also can be used as the additive of solid-state food, as:
The cookies batching is added 0.5～1.0% peptide native powder, produces the peptide native cookies.
The bread batching is added 0.5～1.0% peptide native powder, produces peptide native bread.
The milk powder batching is added 0.5～1.0% peptide native powder, produces peptide native milk powder.
As the additive of liquid beverage, as:
The yoghourt batching is added 0.5～1.0% peptide native powder, produces the peptide native yoghourt.
As the additive of paste food, as:
Autumn pear cream batching is added 0.1～0.5% peptide native powder, produces peptide native autumn pear cream.
As health food or medicinal preparation, use more extensive.As compatible, obtain having the peptide native powder of promoting curative effect with following Chinese medicine.：
Cordyceps 20%, the Radix Astragali 5%, Radix Codonopsis 5%, Fructus Lycii 10%, Colla Corii Asini 10%, peptide native 50%
These materials can better increase the functional characteristic of peptide native.Used Cordyceps can be wild, also can be the mycelium of biotechnology fermenting and producing, and used Chinese medicine is powder or its extract.
The peptide native powder also can with above-mentioned Chinese medicine in any one, any two kinds, any three kinds, any four kinds compatible, preparation peptide native health food or medicinal preparation.
Peptide native health food or medicinal preparation mainly comprise: peptide native coated tablet, peptide native electuary, peptide native capsule, peptide native oral liquid.
Claims (19)
1, a kind of preparation method of peptide native and peptide native series products, it is characterized in that: with the edibility animal blood is raw material, technical process through the blood collecting-former powder of DC separation-one-level conversion-secondary conversion-peptide native-preparation-product, adopt biodegradation method, destroy the protein macromolecule structure, decomposing protein is small peptide and aminoacid, preparation is with polypeptide, aminoacid and heme iron are the former powder of the peptide native of Main Ingredients and Appearance, the former powder of this peptide native has and improves because the effect of the human body iron deficiency anemia that iron deficiency or malabsorption iron deficiency are caused in the food, be additive with the former powder of peptide native again, have iron supplement with the food of different shape or medicine preparation, improve the auxotype anemia, the health food of symptoms such as QI and blood deficiency or medicinal preparation.
2, the preparation method of peptide native according to claim 1, it is characterized in that: it is to adopt the closed blood-sampling method that animal blood is collected, promptly adopt hollow blood sampling knife to connect the sealing mode of flexible pipe and blood sampling bucket successively, 50 liters of collection blood bucket dischargeable capacitys, load-bearing is an optimum for 40 kilograms, and blood sampling time was controlled between 10～40 seconds, and collection process and conveyer chain keep synchronously, be not add anticoagulant in the blood collection procedure, or only add a small amount of anticoagulant.
3, the preparation method of peptide native according to claim 1, it is characterized in that: blood collecting carries out at normal temperatures, collect and carry out DC separation pretreatment within back 4 hours, stirring, broken and homogenizing blood cell structure, form the haemolysis state of three layer components, being to eliminate upper foam with spraying into a small amount of enzyme liquid, is with 40 order net skim middle level impurity, is directly as pending blood plasma with lower floor's blood plasma.
4, the preparation method of peptide native according to claim 1 is characterized in that: aforesaid anticoagulant is 5～40% sodium citrate aqueous solution, and addition is that per 100 milliliters of blood add 1～10 milliliter of anticoagulant.
5, the preparation method of peptide native according to claim 1 is characterized in that: pending blood is through two-stage biological Degradation and Transformation process, reaches and destroys the protein macromolecule structure, and decomposing protein is small peptide and amino acid whose effect.
6 preparation methoies of peptide native according to claim 1 or 5, it is characterized in that: one-level transforms, be in bioreactor slowly under the stirring condition, pending blood is heated to 40-70 ℃, treat temperature stabilization after, slowly add endopeptidase Alcalase2.4L, addition is the 0.1-2.0% of blood plasma volume, kept thermotonus 0.5-6 hour, control DH value 5-15%, pH value ends when 7.5-7.6 drops to 5.8-6.5.
7, the preparation method of peptide native according to claim 1 or 5, it is characterized in that: after the first time, conversion process stopped, under the equal physical condition on this basis, carrying out secondary transforms, promptly adding weakly acidic exopeptidase Flavourzyme1000L does further to repair to small peptide and releases, addition is the 0.1-2.0% of blood plasma volume, continues reaction 0.5-6 hour, and the DH value is controlled at 30-70%.
8, the preparation method of peptide native according to claim 1 or 5, it is characterized in that: in the two-stage conversion process, all be to measure and control DH value (degree of hydrolysis), (h mmol/g) accounts for total peptide bond mM number (h promptly to measure the mM number of peptide bond cleaved in every gram material protein
Tot, percent value mmol/g) determines that its calculating formula is: DH=h/h with enzyme amount, response time and reaction temperature
Tot* 100%.
9, the preparation method of peptide native according to claim 1 or 5 is characterized in that: two-stage transforms the papain that the enzyme preparation that is adopted is the Alcalase endopeptidase that meets the NOVO company that food stage requires, Neutrase endopeptidase, Protamex endopeptidase+exopeptidase, Flavourzyme1000 exopeptidase, Guangzhou enzyme preparation factory.
10, the preparation method of peptide native according to claim 1 is characterized in that: will carry out enzyme denaturing after the secondary conversion process finishes, be 70～90 ℃ of temperature, keeps carrying out under the condition in 10-40 minute.
11, the preparation method of peptide native according to claim 1 is characterized in that: the peptide native oleo stock in the preparation process, and adopt spray drying method to carry out, inlet temperature is 180-220 ℃, leaving air temp 70-80 ℃, dry run keeps stirring.
12, the preparation method of peptide native according to claim 1 is characterized in that: peptide native oleo stock can also adopt the freeze-drying dehydration, preparation peptide native finished product.
13, according to the preparation method and the product of claim 1 and 11,12 described peptide natives, it is characterized in that: the peptide native final finished is Powdered, bronzing, little light bitterness, free from extraneous odour, moisture content≤10%, protein 〉=60%, ferrum 〉=2000mg/kg.
14, peptide native series products according to claim 1 is characterized in that: the peptide native finished product can by every day 2 gram dosage directly as symptoms such as iron deficiency anemia, QI and blood deficiency tonic.
15, peptide native series products according to claim 1, it is characterized in that: the peptide native finished product can be used as the additive of solid-state food, liquid beverage, paste food.
16, peptide native series products according to claim 1 is characterized in that: peptide native finished product and Cordyceps, relevant Chinese medicinal components compatibilities such as the Radix Astragali, Radix Codonopsis, Fructus Lycii, Colla Corii Asini prepare the peptide native medicinal preparation or the health food of agent in many ways.
17, according to claim 1 or 16 described peptide native series products, it is characterized in that: the peptide native finished product be with aforementioned Chinese medicinal components in any one, any two kinds, any three kinds, any four kinds carry out formulated in combination.
18, according to claim 1 or 16 described peptide native series products, it is characterized in that: aforesaid peptide native medicinal preparation or health food are to prepare in proportion with the powder or the extract of relevant Chinese medicinal components.
19 according to claim 1 or 16 described peptide native series products, and it is characterized in that: the main form of peptide native medicinal preparation and health food is the peptide native coated tablet, peptide native electuary, peptide native capsule, peptide native oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99125047A CN1252997A (en) | 1999-11-25 | 1999-11-25 | Process for preparing hemopeptidin and hemopeptidin products |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99125047A CN1252997A (en) | 1999-11-25 | 1999-11-25 | Process for preparing hemopeptidin and hemopeptidin products |
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| CN1252997A true CN1252997A (en) | 2000-05-17 |
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|---|---|---|---|
| CN99125047A Pending CN1252997A (en) | 1999-11-25 | 1999-11-25 | Process for preparing hemopeptidin and hemopeptidin products |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1483978A1 (en) * | 2003-06-04 | 2004-12-08 | Compagnie Generale de Dietetique | Biological process for the production of a food product comprising heme-iron and food product obtained with said process |
| CN101664543B (en) * | 2009-09-02 | 2011-10-26 | 张滨 | Hemokinin-iron oral liquid |
| CN102300471A (en) * | 2009-01-30 | 2011-12-28 | 申泰容 | Blood waste treatment system for slaughtered animals, and method for producing high quality amino acid solution using blood waste |
| CN101748181B (en) * | 2010-01-15 | 2012-12-26 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
| CN102964423A (en) * | 2012-12-05 | 2013-03-13 | 四川四海食品股份有限公司 | Purification process for producing hemoglobin peptides by using pig blood |
| CN102994584A (en) * | 2012-09-24 | 2013-03-27 | 南昌大学 | Method for producing heme iron by using duck blood |
| CN105996009A (en) * | 2016-06-29 | 2016-10-12 | 陈石良 | Antioxidative anti-aging SOD nano-selenium peptide composition and preparation method thereof |
| CN111018867A (en) * | 2019-12-12 | 2020-04-17 | 山东立菲生物产业有限公司 | Method for quickly extracting heme from hemoglobin |
| CN113598377A (en) * | 2021-08-09 | 2021-11-05 | 河南磐康健康管理股份有限公司 | Composition for improving anemia, functional food thereof, preparation method and application |
| WO2023074991A1 (en) * | 2021-10-26 | 2023-05-04 | 주식회사 아미노랩 | Method for production of bioactive substance using blood and application thereof |
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1999
- 1999-11-25 CN CN99125047A patent/CN1252997A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1483978A1 (en) * | 2003-06-04 | 2004-12-08 | Compagnie Generale de Dietetique | Biological process for the production of a food product comprising heme-iron and food product obtained with said process |
| FR2855722A1 (en) * | 2003-06-04 | 2004-12-10 | Cie Generale De Dietetique | BIOLOGICAL PROCESS FOR OBTAINING A HEMIC IRON-BASED FOOD PREPARATION AS WELL AS A FOOD PREPARATION OBTAINED FOLLOWING THE IMPLEMENTATION OF THIS PROCESS |
| CN102300471A (en) * | 2009-01-30 | 2011-12-28 | 申泰容 | Blood waste treatment system for slaughtered animals, and method for producing high quality amino acid solution using blood waste |
| CN101664543B (en) * | 2009-09-02 | 2011-10-26 | 张滨 | Hemokinin-iron oral liquid |
| CN101748181B (en) * | 2010-01-15 | 2012-12-26 | 白求恩医科大学制药厂 | Method for preparing high-ferric content ferroheme polypeptide composite with pepsin hydrolysis method |
| CN102994584A (en) * | 2012-09-24 | 2013-03-27 | 南昌大学 | Method for producing heme iron by using duck blood |
| CN102964423A (en) * | 2012-12-05 | 2013-03-13 | 四川四海食品股份有限公司 | Purification process for producing hemoglobin peptides by using pig blood |
| CN105996009A (en) * | 2016-06-29 | 2016-10-12 | 陈石良 | Antioxidative anti-aging SOD nano-selenium peptide composition and preparation method thereof |
| CN111018867A (en) * | 2019-12-12 | 2020-04-17 | 山东立菲生物产业有限公司 | Method for quickly extracting heme from hemoglobin |
| CN113598377A (en) * | 2021-08-09 | 2021-11-05 | 河南磐康健康管理股份有限公司 | Composition for improving anemia, functional food thereof, preparation method and application |
| WO2023074991A1 (en) * | 2021-10-26 | 2023-05-04 | 주식회사 아미노랩 | Method for production of bioactive substance using blood and application thereof |
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