CN1252243C - Shaker device with functions of making real time detection, controlling pH and supplementing material - Google Patents
Shaker device with functions of making real time detection, controlling pH and supplementing material Download PDFInfo
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- CN1252243C CN1252243C CN 03129230 CN03129230A CN1252243C CN 1252243 C CN1252243 C CN 1252243C CN 03129230 CN03129230 CN 03129230 CN 03129230 A CN03129230 A CN 03129230A CN 1252243 C CN1252243 C CN 1252243C
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Abstract
The present invention discloses a shaker with the functions of real-time detection, pH control and material supplementation. Sensors such as pH electrodes, etc. are arranged in the shake flask as the kernel part of the shaker, and are capable of detecting the pH value (or other parameters) of a culture solution in real time and converting the pH value into electric signals which are transmitted to an electronic measuring and controlling device; when the pH value of the culture solution deviates from a set value by (+/-)0.1, the electronic measuring and controlling device gives instructions to open an electromagnetic valve in 0.2 second so as to enable the feed solution in a feeding bottle to enter the shake flask from the feeding opening at one side of the shake flask through an elastic feeding tube and a feeding needle. When the pH restores to the preset value, the electronic measuring and controlling device automatically gives instructions to close the electromagnetic valve; 2 to 4 baffles are tightly attached to the wall of the bottle in the position of the cone angle of the bottom inside the shake flask, and the function of the baffles aims at improving the mixing uniformity of cultured components in the shake flask and the condition of the dissolved oxygen so as to enhance the precision of a measured pH value.
Description
Technical field
The present invention relates to the shaking table of a kind of bacterial screening and cultivation, particularly a kind of microorganism, improved shaking table of animal and plant cells bacterial screening and seed enlarged culturing aspect of being used for.
Background technology
Shaking table is a kind of specific equipment indispensable in the bioprocesses.Be used for aspects such as microorganism, animal and plant cells bacterial screening, seed enlarged culturing.Be extensive use of in fields such as biochemistry, medicine, food, light industry, environmental protection, agricultural chemicals.As far back as 20th century the '30s shaking table bring into use, established technical foundation for the submerged-culture method that grew up afterwards, development of biology has been made indelible contribution.So far still bringing into play irreplaceable effect.At present, traditional shaking table has the function of speed control, temperature control through technique improvement.But bioprocesses is to take place on three levels of transmission characteristic of the metabolism adjusting of hereditary property, cell levels at molecular level and engineering level, is the process of this three aspects comprehensive action.And certain temperature is only controlled in existing shaking table cultivation and rotating speed is far from being enough, and these measurements or control do not reflect the variation of above-mentioned situation.Especially in the shake-flask culture process, pH changes the overall target that comes down to thalline physiological metabolism and the variation of reactor (shaking bottle) mixing transmittance process, if we can research and analyse the H balance in the shake-flask culture process, just can understand the relation of various cultivation compositions and bacterial classification physiological property, for further optimizing fermentation provides necessary parameter and basis.Therefore, branch of industry wishes that relevant engineering technical personnel further improve existing shaking table, to satisfy many-sided requirement of bioprocesses.
Summary of the invention
Purpose of the present invention:
For overcoming existing shaking table in the defective aspect the cultivation, designed a kind of shaking table with real-time detection, control pH and feed supplement function, can satisfy the requirement of each side in the bioprocesses.
Design of the present invention is such:
As everyone knows: shaking table mainly by machinery concussion mechanism, shakes parts such as bottle and electronic measurement and control system and forms.Wherein shaking bottle is the core component of bioprocesses.The present invention is exactly the defective that exists at existing shaking table to the core component of shaking table-shake bottle to improve, and its design is:
1, by pH detecting electrode (or other transmitter is set in shaking bottle, as dissolved oxygen electrode, nutrient solution concentration determination electrode and target product concentration determination electrode etc.), in culturing process, the pH (or other parameter) that shakes bottle interior nutrient solution changes, by detecting electrode signal is imported in the electronic measurement and control device, but The real time measure with the record each shake the pH value (or other parameter value) of bottle interior nutrient solution.In addition, microbial strains grows into certain stage in shaking bottle, when the pH of nutrient solution changes to a certain value, electronic measuring device also can be when real-time continuous be measured record, send the signal of startup (or closing) magnetic valve, nutrient solution in the feed supplement bottle is added dropwise to by elastic material pipe trace shakes in the bottle;
2, automatic feeding (or control pH) device: when operations of shaker, can be by the switch of magnetic valve, shake bottle with the feed liquid inflow of blocking or opening in the feed supplement pipe, mending the amount and the time of going into can be by manually giving first setting in computer, the purpose that also can reach the dropping feed liquid by the signal control magnetic valve that shakes a bottle interior pH electrode (or other transmitter) or regulate pH;
3, shake bottle baffle plate of inner bottom part cone angle by being arranged on, improve the concussion mixture strength of nutrient solution, the cultivation component is in all the time mixes attitude, thus the accuracy that improves pH value (or other value).The present invention realizes according to above-mentioned design, and a kind of shaking table with real-time detection, control pH and feed supplement function mainly by machinery concussion mechanism, shakes bottle and electronic measurement and control system and forms; Wherein shaking bottle is the core component of bioprocesses, it is characterized in that:
In shaking bottle, be provided with the real-time detecting electrode of pH, said electrode shakes in the bottle by the rubber stopper seal insertion of shaking in the electrode socket on bottle one side (as the right side), one end nationality of electrode helps protector protected, prevent that electrode is bruised in the earthquake process, the other end of electrode passes to the electronic measurement and control device by lead with signal;
Be provided with material-feeding port at the opposite side (as the left side) that shakes bottle, material-feeding port seals with the medical grade rubber plug; The feed supplement syringe needle that one end of feed supplement pipe is connected with similar medical needle shakes in the bottle by the rubber plug insertion, the other end of feed supplement pipe links to each other with the feed supplement bottle, middle nationality helps the keying control feed supplement of magnetic valve, the keying of magnetic valve is then undertaken by the instruction of electronic measurement and control device, with shake the bottle cone angle place, bottom, be close to a bottle wall and establish 2～4 baffle plates.Said electronic measurement and control device is made up of impedance transformer, controller and indicating meter.Said baffle plate, its material are a kind of in glass, stainless steel or the engineering plastics plate.In shaking bottle, except with pH electrode, can also detect in real time with dissolved oxygen electrode, nutrient solution concentration determination electrode or target product concentration determination electrode.It is further embodied in the following drawings and Examples.
Description of drawings
Fig. 1 shaking table overall operation synoptic diagram
Wherein:
1-feed supplement bottle, the required additional feed liquid of interior storage microorganism growth or be used to adjust the reagent of pH;
The 2-impedance transformer;
The 3-controller;
The 4-indicating meter;
5-magnetic valve, its internal structure accompanying drawing are in addition showed;
6-shakes bottle, and its internal structure accompanying drawing is in addition showed;
7-pH electrode (or other transmitter);
The 8-material-feeding port;
9-shaking table chassis;
The 10-bent axle operates by reduction gearing mechanism motor drives (not shown).
As seen from Figure 1: shaking table of the present invention, wherein each shakes bottle 6 and is provided with pH electrode 7, the pH value of shaking the nutrient solution in the bottle is converted into electric current by electrode, transfer to separately impedance transformer 2 by lead respectively again, and then deliver to controller 3 and indicating meter 4 respectively, can show respectively and a certain specific pH value of shaking in the bottle of record by button on the indicating meter, when the nutrient solution in shaking bottle departs from a certain pH value ± 0.1, controller 3 just can send the magnetic valve 5 on certain feed supplement pipe of instruction unlatching respectively in 0.2 second, nutrient solution in the feed supplement bottle then flows to material-feeding port 8 by the feed supplement pipe, and enter and shake bottle by feed supplement syringe needle (a kind of medical needle that is similar to), pH value in shaking bottle returns in the preset range, controller will cut out magnetic valve 5 by automatic command, stops feed supplement.
Because pH electrode 7 (or dissolved oxygen electrode, nutrient solution concentration determination electrode and target product concentration determination electrode etc.), impedance transformer 2, controller 3 and indicating meter 4 all are commercially available, so its description is omitted.
Said electronic measurement and control device is made up of impedance transformer 2, controller 3 and indicating meter 4 all members, perhaps imports computer, is regulated and control by computer.
Fig. 2 is for shaking the bottle structure synoptic diagram
Wherein:
The 1a-material-feeding port;
2a-medical rubber plug;
3a-sterile seal plug;
The 4a-rubber plug;
The 5a-transmitter can be pH electrode or other transmitter;
6a-electrode socket;
The 7a-baffle plate is close to and is shaken a bottle inner bottom part cone angle, and quantity is 2～4; It can be that the glass baffle plate is fixed in cone angle place, bottom, also can use highly elastic material, manufactures as stainless steel substrates or engineering plastics to form;
The 8a-protector.
The work synoptic diagram of Fig. 3 automatic feeding and magnetic valve
Wherein:
The 1b-pipe clamp, nationality helps the armature in the magnetic valve that elasticity feed supplement pipe is held out against in pipe clamp, to close the feed liquid path, is in the state of closing among the figure;
2b-elasticity feed supplement pipe, its end connects the feed supplement bottle, and the other end links to each other with syringe needle to insert and shakes in the bottle;
The 3b-retaining plate is equipped with parts such as pipe clamp on it;
4b-shaking table shell;
Armature member in the 5b-magnetic valve;
6b-top spring;
The 7b-electro-magnet.
Shaking table of the present invention has following technique effect through practical probation:
1, solves the problem that traditional shake flask fermentation culturing process can't same of continuously measured be shaken growth parameter(s) in the bottle (as pH etc.), improve the reliability of take off data;
(as regulating pH, feed supplement etc.) essential aseptic technique and caused growing environment variation issue of shutdown operation process when 2, changing culture condition in the traditional shake flask fermentation process of solution, and manually sequential setting in advance and accurately control;
But 3, wish to change, adjust culture condition, reduce the blindness of operation by the real-time parameter of continuously measured;
4, improve the mixing and the dissolved oxygen situation of traditional shake-flask culture process nutrient solution;
5, under identical starting condition, test different culture condition simultaneously, be convenient to research;
6, easy and simple to handle, cut down the consumption of energy, save cost;
7, on the basis of the above, can compare the early stage research of the metabolic characteristic and the optimizing fermentation of different strain simultaneously, promptly be used for the back technical study of bacterial screening and strain improvement.
Embodiment
Further illustrate the content of invention below in conjunction with embodiment, but these embodiment do not limit protection scope of the present invention.
Embodiment 1
For optimizing culture condition, seek suitable medium, use device of the present invention to cultivate pichia spp:
With initial pH is that 4.0 pichia spp nutrient solution is divided into three groups (n=2) and places this shaking table to cultivate: group is not controlled pH, and two groups pH value is set at 5.0,5.5 respectively, span of control in addition :+/-0.1.When runawaying, magnetic valve is opened, cp=20 second, p=0.2 second.Behind the 48h, testing data sees the following form during cultivation:
| pH | Do not control | 5.0 | 5.5 |
| PH behind the 48h | 3.4 | 5.1 | 5.6 |
The testing data explanation, pH was at 5.0,5.5 o'clock in control, and 48h secondary fermentation liquid pH remains in the span of control, reaches processing requirement.Simultaneously easy and simple to handle, increase work efficiency.
Embodiment 2
For optimizing culture condition, seek suitable medium, use device of the present invention to cultivate nanchang streptomycete:
In fermentor tank, cultivate and finish nanchang streptomycete to vegetative period (48h), fermented liquid is divided into three groups (n=2) places this shaking table to cultivate: control different pH (pH 7.0, pH 7.5, pH 8.0) respectively, span of control :+/-0.1.When runawaying, magnetic valve is opened, cp=20 second, p=0.2 second.Be cultured to measure behind the 120h and tire, testing data sees the following form:
| pH | 7.0 | 7.5 | 8.0 |
| PH behind the 120h | 7.1 | 7.6 | 8.1 |
| (μ g/ml) tires behind the 120h | 36 | 52 | 43 |
Testing data explanation, use this shaker fermentation to cultivate 120h after, pH can remain in the span of control, test-results provides Useful Information for seeking the culture condition of optimizing simultaneously.
In addition, shaking table of the present invention has also been obtained significant effect at the aspects such as optimization of screening, substratum and the culture condition of other bacterial classification.Data are reliable, easy and simple to handle, efficient and low-cost, are outstanding advantages of the present invention.The present invention will provide more science, effective testing data for zymotechnique amplifies if be used in combination with modern bio-reactor, to understanding bioprocesses tool directive significance, be with a wide range of applications.
In a word, because technological improvement of the present invention brings new vitality to shaking table, the more perfect function of shaking table, thus shaking table is played a greater role in biotech development, and promote bionic technical progress effectively.
Claims (4)
1, a kind of shaking table with real-time detection, control pH and feed supplement function mainly by machinery concussion mechanism, shakes bottle and electronic measurement and control system and forms; Wherein shaking bottle is the core component of bioprocesses, it is characterized in that:
(1) in shaking bottle, is provided with the real-time detecting electrode of pH, the real-time detecting electrode of said pH (5a) shakes in the bottle by rubber plug (4a) the sealing insertion of shaking in the electrode socket (6a) on bottle one side, one end nationality of electrode helps protector (8a) to be protected, prevent that electrode is bruised in the earthquake process, the other end of electrode passes to the electronic measurement and control device by lead with signal;
(2) be provided with material-feeding port (1a) at the opposite side that shakes bottle, material-feeding port (1a) seals with medical grade rubber plug (2a); The feed supplement syringe needle that one end of feed supplement pipe is connected with similar medical needle shakes in the bottle by rubber plug (2a) insertion, the other end of feed supplement pipe links to each other with feed supplement bottle (1), middle nationality helps the keying control feed supplement of magnetic valve, the keying of magnetic valve is then undertaken by the instruction of electronic measurement and control device, with shake the bottle cone angle place, bottom, be close to a bottle wall and establish 2～4 baffle plates (7a).
2, shaking table as claimed in claim 1 is characterized in that said electronic measurement and control device is made up of impedance transformer (2), controller (3) and indicating meter (4).
3, shaking table as claimed in claim 1 is characterized in that (2) said baffle plate (7a), and its material is a kind of in glass, stainless steel or the engineering plastics plate.
4, shaking table as claimed in claim 1 is characterized in that except pH electrode, can also use dissolved oxygen electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03129230 CN1252243C (en) | 2003-06-13 | 2003-06-13 | Shaker device with functions of making real time detection, controlling pH and supplementing material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03129230 CN1252243C (en) | 2003-06-13 | 2003-06-13 | Shaker device with functions of making real time detection, controlling pH and supplementing material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1460710A CN1460710A (en) | 2003-12-10 |
| CN1252243C true CN1252243C (en) | 2006-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03129230 Expired - Fee Related CN1252243C (en) | 2003-06-13 | 2003-06-13 | Shaker device with functions of making real time detection, controlling pH and supplementing material |
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| CN (1) | CN1252243C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108018204A (en) * | 2016-11-03 | 2018-05-11 | 浙江金仪盛世生物工程有限公司 | A kind of parallel bioreactor system |
| CN109576183B (en) * | 2018-12-24 | 2022-08-30 | 上海智城分析仪器制造有限公司 | Siphon catheter feeding and shaking table culture method for bacteria |
| CN109609392B (en) * | 2018-12-24 | 2022-04-19 | 上海智城分析仪器制造有限公司 | Siphon catheter feeding table cultivation method for yeast |
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2003
- 2003-06-13 CN CN 03129230 patent/CN1252243C/en not_active Expired - Fee Related
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| CN1460710A (en) | 2003-12-10 |
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| PB01 | Publication | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20170516 Address after: 201400, No. 2222 West Ring Road, South Bridge Town, Shanghai, Fengxian District Patentee after: Shanghai ZHICHENG Anaiytical Instrument Manufacturing Co., Ltd. Address before: Changning District, Shanghai city 200231 West Xianxia Road No. 585 Patentee before: Guoqiang Biochemical Engineering Equipment Co., Ltd., Shanghai |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060419 Termination date: 20210613 |