CN1240828A - Process for extracting detoxicating enzyme for aflatoxin B1 - Google Patents

Abstract

A process for extracting the detoxicating enzyme of aflatoxin B1 features use of an edible fungus, Armillariella, as raw material and includes destroying the thallus to obtain kytoplam liquid, precipitating with 30-85% ammonium sulfate and dialyzing. Its advantages are efficient and special detoxicating effect on aflatoxin B1, no toxicity.

Description

AFB 1Process for extracting detoxicating enzyme

The present invention relates to a kind of toxenzyme of separating, particularly AFB ₁Process for extracting detoxicating enzyme.

Remove or transform AFB in the prior art ₁Method (1) chemical method is arranged: use clorox, ozone, hydrogen peroxide, sodium hydroxide, formaldehyde, ammonium salt and chlorine etc., main drawback is to destroy AFB ₁The time also destroyed the many nutritions in the food, as VITAMIN, protein, alkaloid, and usually can influence the color (when especially using chlorine or ammonium salt) of food or seasonings, some method can't be used for water and handle; (2) physical-chemical method: these class methods are to be used in combination with chemical process with r-ray, uviolizing, and its shortcoming and chemical method are similar; (3) physisorphtion: these class methods are to use technology such as diatomite, ultrafiltration to remove AFB in the liquid-food ₁Or M1, the shortcoming of this method is a complex process, is difficult to suitability for industrialized production, effect is not very good, and when using, causes the loss of food nutrition composition inevitably; (4) biotransformation method in the body, this method is with some chemical reagent such as 2-Butylated Hydroxytoluene, Oltipraz etc., liver paddy Guang peptidyl transferase increases in the feeding animals inductor, to increase the AFB in the liver ₁Conversion, its shortcoming is to rely on the cells in vivo pigment P ₄₅₀Existence; To AFB ₁Detoxifcation be to be activated at aflatoxin just to detoxify after toxic; Inductor itself is just toxic; This method can not be used for food processing process.

Purpose of the present invention is intended to overcome the deficiencies in the prior art and provides a kind of single-minded, efficient, nontoxic and can be directly to AFB ₁The toxicide process for extracting detoxicating enzyme.

The object of the present invention is achieved like this:

A kind of AFB ₁Separate the extraction process of toxenzyme, it is characterized in that cultivating 5-70 days at 25-30 ℃ with Armillariella tabescens (Armillariella Fabescens), take out thalline, heavy with thalline: damping fluid (PH6)=1: 1 (weight in wet base: volume), add the damping fluid of PH6, with mechanical mill or ultrasonic, or the high pressure breaking method destroys thalline, its cytosol flowed out, then, the centrifuging and taking supernatant liquor, behind the ammonium sulfate precipitation with 30-85%,, promptly get AFB with damping fluid or distill water dialysis 4-24 hour desalination ₁Separate toxenzyme.

The invention will be further described below by embodiment:

Get 50g Armillariella tabescens bacterial classification inoculation in comprehensive potato culture, be cultured to about 28 days of growth animated period under 28 ℃, filter and take out thalline, about 1000g weighs, mix with the phosphoric acid buffer of 1000mlPH6, with mechanical mill, or high pressure, or the ultrasonic disruption thalline, cytosol is flowed out, through the about 1000ml of centrifugal taking-up supernatant liquor, the ammonium sulfate precipitation with 50% promptly gets AFB with distill water dialysis after 10 hours ₁Separate toxenzyme.

Technology of the present invention is extracted separates toxenzyme, is a kind of biological enzyme, to AFB ₁Specificity is arranged, and high efficiency can be directly to its detoxifcation, because this detoxifcation enzyme source is in edible fungus, thus nontoxic, safe to eat, because edible fungus is originated enriches, can expand as suitability for industrialized production in addition.

Claims (1)

1, a kind of AFB ₁Separate the extraction process of toxenzyme, it is characterized in that cultivating 5-70 days at 25-30 ℃ with Armillariella tabescens (Armillariella tabescens), take out thalline, heavy with thalline: damping fluid (PH6)=1: 1 (weight in wet base: volume), add the damping fluid of PH6, thalline is destroyed with mechanical mill or ultrasonic or high pressure breaking method, its cytosol is flowed out, then, the centrifuging and taking supernatant liquor is with behind the ammonium sulfate precipitation of 30-85%, with damping fluid or distill water dialysis 4-24 hour desalination, promptly get AFB ₁Separate toxenzyme.