CN1113098C - Process for extracting detoxicating enzyme for aflatoxin B1 - Google Patents
Process for extracting detoxicating enzyme for aflatoxin B1 Download PDFInfo
- Publication number
- CN1113098C CN1113098C CN98113268A CN98113268A CN1113098C CN 1113098 C CN1113098 C CN 1113098C CN 98113268 A CN98113268 A CN 98113268A CN 98113268 A CN98113268 A CN 98113268A CN 1113098 C CN1113098 C CN 1113098C
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- China
- Prior art keywords
- enzyme
- extracting
- aflatoxin
- thalline
- damping fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The present invention provides a technique for extracting aflatoxin B1 detoxifcation enzyme, which is characterized in that false shoestring fungus of edible fungus is used for damaging mycelium, the cytosol is precipitated and dialyzed by 30 to 85% of ammonium sulfate, and thus, detoxification enzyme is prepared. The detoxification enzyme has singleness and high efficiency to aflatoxin B1 and has the advantages of no toxin, and safe eating. Because the edible fungus has wide sources, the present invention can be enlarged into industrialized extracting techniques.
Description
The present invention relates to a kind of toxenzyme of separating, particularly AFB
1Process for extracting detoxicating enzyme.
Remove or transform AFB in the prior art
1Method (1) chemical method is arranged; Use clorox, ozone, hydrogen peroxide, sodium hydroxide, formaldehyde, ammonium salt and chlorine etc., main drawback is to destroy AFB
1The time also destroyed the many nutritions in the food, as VITAMIN, protein, alkaloid, and usually can influence the color (when especially using chlorine or ammonium salt) of food or seasonings, some method can't be used for water and handle; (3) physics-chemical method: these class methods are to be used in combination with chemical process with r-ray, uviolizing, and its shortcoming and chemical method are similar; (3) physisorphtion: these class methods are to use technology such as diatomite, ultrafiltration to remove AFB in the liquid-food
1Or M1, the shortcoming of this method is a complex process, is difficult to suitability for industrialized production, effect is not very good, and when using, causes the loss of food nutrition composition inevitably; (4) biotransformation method in the body, this method is with some chemical reagent such as 2-Butylated Hydroxytoluene, Oltipraz etc., liver paddy Guang peptidyl transferase increases in the feeding animals inductor, to increase the AFB in the liver
1Conversion, its shortcoming is to rely on the cells in vivo pigment P
450Existence; To AFB
1Detoxifcation be to be activated at aflatoxin just to detoxify after toxic; Inductor itself is just toxic; This method can not be used for food processing process.
Purpose of the present invention is intended to overcome the deficiencies in the prior art and provides a kind of single-minded, efficient, nontoxic and can be directly to AFB
1The toxicide process for extracting detoxicating enzyme.
The object of the present invention is achieved like this:
A kind of AFB
1Separate the extraction process of toxenzyme, it is characterized in that cultivating 5-70 days at 25-30 ℃ with Armillariella tabescens (Araillatiella fabescens), it is heavy with thalline to take out thalline: damping fluid (PH6)=1: 1 (weight in wet base: volume), add the damping fluid of PH6, with mechanical mill or ultrasonic, or the high pressure breaking method destroys thalline, its cytosol is flowed out, then, the centrifuging and taking supernatant liquor is with behind the ammonium sulfate precipitation of 30-85%, with damping fluid or distill water dialysis 4-24 hour desalination, promptly get AFB
1Separate toxenzyme.
The invention will be further described below by embodiment:
Get 50g Armillariella tabescens bacterial classification inoculation in comprehensive potato culture, be cultured to about 28 days of growth animated period under 28 ℃, filter and take out thalline, about 1000g weighs, mix with the phosphoric acid buffer of 1000mlPH6, with mechanical mill, or high pressure, or the ultrasonic disruption thalline, cytosol is flowed out, through the about 1000ml of centrifugal taking-up supernatant liquor, the ammonium sulfate precipitation with 50% promptly gets AFB with distill water dialysis after 10 hours
1Separate toxenzyme.
Technology of the present invention is extracted separates toxenzyme, is a kind of biological enzyme, to AFB
1Specificity is arranged, and high efficiency can be directly to its detoxifcation, because this detoxifcation enzyme source is in edible fungus, thus nontoxic, safe to eat, because edible fungus is originated enriches, can expand as suitability for industrialized production in addition.
Claims (1)
1, a kind of flavacin B
1The toxicide extraction process is characterized in that cultivating 5-70 days at 25-30 ℃ with Armillariella tabescens (Armillariella tabescens), take out thalline, with thalline heavy (g): pH60 damping fluid volume (ml)=1: 1, add the damping fluid of pH6.0, with mechanical mill or ultrasonic or high pressure breaking method thalline is destroyed, its cytosol is flowed out, then, the centrifuging and taking supernatant liquor is with behind the ammonium sulfate precipitation of 30-85%, with damping fluid or distill water dialysis 4-24 hour desalination, promptly get flavacin B
1Separate toxenzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98113268A CN1113098C (en) | 1998-06-30 | 1998-06-30 | Process for extracting detoxicating enzyme for aflatoxin B1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98113268A CN1113098C (en) | 1998-06-30 | 1998-06-30 | Process for extracting detoxicating enzyme for aflatoxin B1 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1240828A CN1240828A (en) | 2000-01-12 |
| CN1113098C true CN1113098C (en) | 2003-07-02 |
Family
ID=5223017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98113268A Expired - Fee Related CN1113098C (en) | 1998-06-30 | 1998-06-30 | Process for extracting detoxicating enzyme for aflatoxin B1 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1113098C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100339703C (en) * | 2004-07-09 | 2007-09-26 | 广州科仁生物工程有限公司 | Biosensor electrode used for detecting aflatoxin and variegated aspergillin and its preparation method |
| CN1294255C (en) | 2004-08-17 | 2007-01-10 | 广州科仁生物工程有限公司 | Toxicide enzyme with aflatoxin activity conversion and gene for coding the enzyme |
| CN102687800B (en) * | 2012-05-03 | 2013-06-19 | 山东农业大学 | Preparation method of aflatoxin B1 biological degradation agent and application thereof |
| CN103947847B (en) * | 2014-03-31 | 2016-01-13 | 宁夏乙征生物工程有限公司 | The detoxication enzyme of aflatoxin in a kind of feed |
| CN104130984B (en) * | 2014-07-30 | 2016-09-28 | 暨南大学 | Act on the aflatoxin oxidase of versicolorin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047786A (en) * | 1989-06-05 | 1990-12-19 | 广西田东县化工厂 | The production technology and the prescription of " going the aflatoxin spirit " |
| CN1152999A (en) * | 1995-12-28 | 1997-07-02 | 洛阳科宝化工有限公司 | Aflatoxins removing agent and its preparing process |
-
1998
- 1998-06-30 CN CN98113268A patent/CN1113098C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047786A (en) * | 1989-06-05 | 1990-12-19 | 广西田东县化工厂 | The production technology and the prescription of " going the aflatoxin spirit " |
| CN1152999A (en) * | 1995-12-28 | 1997-07-02 | 洛阳科宝化工有限公司 | Aflatoxins removing agent and its preparing process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1240828A (en) | 2000-01-12 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |