CN1226419C - Prepn of natural active protein and peptide - Google Patents
Prepn of natural active protein and peptide Download PDFInfo
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- CN1226419C CN1226419C CN 02133462 CN02133462A CN1226419C CN 1226419 C CN1226419 C CN 1226419C CN 02133462 CN02133462 CN 02133462 CN 02133462 A CN02133462 A CN 02133462A CN 1226419 C CN1226419 C CN 1226419C
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- reaction
- raw material
- natural
- peptide
- enzymolysis
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 38
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 23
- 108091005804 Peptidases Proteins 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000000047 product Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
- 239000007795 chemical reaction product Substances 0.000 claims abstract description 13
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 239000008367 deionised water Substances 0.000 claims abstract description 8
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000005238 degreasing Methods 0.000 claims abstract description 7
- 238000009826 distribution Methods 0.000 claims abstract description 6
- 238000007710 freezing Methods 0.000 claims abstract description 6
- 230000008014 freezing Effects 0.000 claims abstract description 6
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 6
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims abstract description 6
- 239000002253 acid Substances 0.000 claims abstract description 5
- 239000004365 Protease Substances 0.000 claims description 22
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 241001465754 Metazoa Species 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 11
- 238000005842 biochemical reaction Methods 0.000 claims description 9
- 238000001976 enzyme digestion Methods 0.000 claims description 9
- 230000000813 microbial effect Effects 0.000 claims description 8
- 244000068988 Glycine max Species 0.000 claims description 7
- 235000010469 Glycine max Nutrition 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 230000009849 deactivation Effects 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 235000014103 egg white Nutrition 0.000 claims description 4
- 210000000969 egg white Anatomy 0.000 claims description 4
- 238000002203 pretreatment Methods 0.000 claims description 4
- 210000002784 stomach Anatomy 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 abstract description 5
- 102000035195 Peptidases Human genes 0.000 abstract description 3
- 235000019833 protease Nutrition 0.000 abstract 2
- 150000007513 acids Chemical class 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 230000008569 process Effects 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 5
- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
Abstract
The present invention discloses a preparation method of a natural active protein and a peptide, which comprises: a closely inspected natural raw material rich in proteins is selected and preprocessed by degreasing, cleaning, etc., and the natural raw material is homogenized; 100 parts by weight of homogenized raw material and 100 to 250 parts by weight of deionized water are added to a biochemical reactor, Ph valve is adjusted to 5 to 6 with acids or alkalis, temperature is increased to 70 to 90 DEG C and maintained, and the mixture is stirred for 10 to 30 minutes and quickly cooled to 30 to 50 DEG C; 0.02 to 0.5 parts by weight of multi-component composite proteinases is added and stirred uniformly for an enzymolysis reaction for 2 to 6 hours, the reaction products are sampled once every 0.5 hour, and the molecular weight distribution of the reaction products is measured by the SDS-PAGE or HPLC/MS method; according to requirements for the molecular weight of the target product, the enzymolysis degree of the natural protein raw material is judged, the time of terminating the enzymolysis reaction is determined, and the proteinases is deactivated immediately after the reaction is terminated; the reaction product are filtered and preserve by freezing.
Description
One, technical field
The present invention relates to the preparation method of natural active protein and peptide, belong to the biological products manufacture field.
Two, background technology
In recent years, the progress of natural proteinoid and peptide is rapid, and people self are to the continuous increase of pure natural product demand, the fashion that the pure natural property material has become people to pursue, and various is that the product of raw material also arises at the historic moment with the pure natural animals and plants.But because of the complex process of preparation natural active protein and peptide, efficient is low, and the product self stability is poor, and cost is high, thereby restricts the widespread use of this product.The analysis reason is as follows:
(1) no matter from 26S Proteasome Structure and Function, the compound that natural bioactive protein and peptide are the occurring in nature kinds, function is the most complicated, thereby it is not strong to separate the actual operation obtain a certain class natural protein or peptide from natural matter;
(2) complex process, agents useful for same and conditional request are comparatively harsh, must keep the proteinic peculiar structure of pure natural, could keep its functions peculiar and biologic activity;
(3) contain the material instability of protein and peptide product, proteinaceous product is subject to proteasome degradation, has increased many labile factors.
China is large agricultural country, and the natural matter of rich in proteins such as animal skin, egg white, soybean or corn is quite abundant.Developed country is widely used in medical material, cosmetics and skincare product, protective foods and functional foodstuff with natural protein and peptide; But China also rests on the roughing stage to its application present stage, and added value is low, causes the wasting of resources and environmental pollution simultaneously.
The biologically active function is the key property of protein and peptide, and the biological activity of protein and peptide depends primarily on its specific three-dimensional arrangement and conformation.In case after three-dimensional arrangement that these are specific and conformation are destroyed, protein will lose activity.General protease method when preparation natural protein and peptide, the inefficiency of protolysate raw material, and required time is long; And the technology that conventional acid hydrolysis and alkali hydrolysis method prepare natural protein and peptide, though the efficient height of protolysate raw material, but its inherent three-dimensional arrangement and conformation are suffered serious destruction to a great extent, and some amino acid may be decomposed simultaneously, thereby makes its product lose biological activity; Acid hydrolysis and basic hydrolysis technology also can be brought environmental pollution in addition.
Three, summary of the invention
The objective of the invention is to provide the preparation method of a kind of natural active protein and peptide at the deficiencies in the prior art, be characterized in using the microbial protease of edible safety and the efficient multicomponent compound protease that various plants, animal protease mix, adopt gentle biochemical reaction system, the activity of considering natural protein and peptide emphatically reclaims, and helps the industrialization of biological activity and functional protein and peptide preparation.
Purpose of the present invention is realized that by following technical measures wherein said raw material umber is parts by weight except that specified otherwise.
The recipe ingredient of natural active protein and peptide preparation is:
100 parts of the natural matters of rich in proteins
100～250 parts of deionized waters
0.02～0.5 part of polynary compound protease
Wherein, the natural matter of rich in proteins is at least a in animal skin, egg white, soybean or the corn; Polynary compound protease is at least a in microbial protease, plant protease and the animal protease.
The preparation method of natural active protein and peptide:
1. will be through strict check be rich in proteinic animal or plant natural matter, animal skin 1-5%Na
2CO
3Solution carries out degreasing, and soybean and corn are used rinsed with deionized water 3 times with the degreasing of physical squeezing method;
2. through 100～250 parts of 100 parts of the proteinaceous natural matters of above-mentioned pre-treatment and homogenate and deionized waters, add in the biochemical reactor;
3. regulate pH=5～8 with acid or alkali, be warming up to 70～90 ℃, insulated and stirred 10～30 minutes is cooled to 30～50 ℃ then rapidly;
4. add 0.02～0.5 part of polynary compound protease in biochemical reactor, after stirring, enzyme digestion reaction 2～6 hours;
5. sampling in per 0.5 hour once adopts SDS-PAGE or HPLC/MS method to measure the molecular weight distribution of biochemical reaction product, and according to the requirement to target product molecular weight size, judgement native protein raw material is determined the enzymolysis reaction time by the degree of enzymolysis;
6. with enzyme deactivation, reaction product is filtered the freezing preservation in back.
The present invention has following advantage:
1. abandon conventional acid system, alkaline process and general enzyme process, the enzyme of use be eat, efficient multicomponent compound protease that the microbial protease of safety and various plants or animal protease mix;
2. before the enzyme digestion reaction, animal collagen 70～90 ℃ of insulated and stirred effects 10～30 minutes, can be changed the senior spirane structure of collagen, be beneficial to effective enzymolysis of proteolytic enzyme, warm-up time is short, and is very little to the activity influence of natural protein and peptide;
3. isolated microbial proteinous endonuclease capable acts on senior spirane structure such as collagen effectively from the natural protein leavened food, so this enzyme particularly to collagen protein, has good enzymolysis to multiple natural protein;
4. owing to added microorganism efficient protein enzyme in the polynary prozyme, other multiple protein enzymes have been added again, these enzymes act synergistically in the process of degrade proteins mutually, the native protein raw material of degrading more efficiently, and this is that other single enzymes or general prozyme can not reach;
5. according to target product control biochemical reaction process, the peptide chain in the directional enzymatic native protein raw material;
6. adopt gentle biochemical reaction system, prepared protein and peptide can keep its specific three-dimensional arrangement and conformation effectively, to guarantee its bioactive existence;
7. because the present invention abandons the shortcoming of conventional acid system, alkaline process and general enzyme process, its technical process, the native protein raw material of can not only degrading effectively, and be friendly to environment;
8. the employed enzyme of biochemical reaction of the present invention all is edible, so its product is a safe green.
Four, embodiment
Below by embodiment the present invention is carried out concrete description; be necessary to be pointed out that at this following examples only are used for the present invention is further detailed; but can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the content of the invention described above.
Embodiment 1
(1) animal skin 3%Na
2CO
3Solution carries out degreasing, uses rinsed with deionized water 3 times;
(2) with after the pretreated animal skin double centner homogenate, mix for 150 kilograms, join in the biochemical reactor with deionized water;
(3) regulate pH=7, stir maintenance 10 minutes for 85 ℃, be cooled to 40 ℃ rapidly in temperature;
(4) add polynary compound protease (by microbial protease, papoid, bromeline, stomach en-and trypsinase approximately by 6: 1: 1: 1: 1 mixed) 20 restrain in biochemical reactor, after stirring, enzyme digestion reaction 2～6 hours;
(5) got one time sample in the enzymolysis process in per 0.5 hour, and adopt SDS-PAGE or HPLC/MS method to measure the molecular weight distribution situation of biochemical reaction product,, determine the enzyme digestion reaction termination time according to requirement to target product molecular weight size;
(6) with enzyme deactivation, reaction product is filtered, freezing preservation.
Embodiment 2
(1) with egg white double centner and deionized water double centner, join in the biochemical reactor behind the thorough mixing;
(2) regulate pH=7, be warming up to 40 ℃;
(3) add polynary compound protease (by microbial protease, papoid, bromeline, stomach en-and trypsinase approximately by 6: 1: 1: 1: 1 mixed) 20 grams in biochemical reactor, after stirring, enzyme digestion reaction 2～6 hours;
(4) got one time sample in the enzymolysis process in per 0.5 hour, and adopt SDS-PAGE or HPLC/MS method to measure the molecular weight distribution situation of biochemical reaction product,, determine the enzyme digestion reaction termination time according to requirement to target product molecular weight size;
(5) with enzyme deactivation, reaction product is filtered, freezing preservation.
Embodiment 3
(1) will clean through the soybean or the corn of strict check, pre-treatment such as squeezing, degreasing;
(2) with pretreated soybean or the homogenate of corn double centner, mix, join in the biochemical reactor with the deionized water double centner;
(3) regulate pH=7, be warming up to 40 ℃;
(4) add polynary compound protease (by microbial protease, papoid, bromeline, stomach en-and trypsinase approximately by 6: 1: 1: 1: 1 mixed) 25 restrain in biochemical reactor, after stirring, enzyme digestion reaction 2～6 hours;
(5) got one time sample in the enzymolysis process in per 0.5 hour, and adopt SDS-PAGE or HPLC/MS method to measure the molecular weight distribution situation of biochemical reaction product,, determine the enzyme digestion reaction termination time according to requirement to target product molecular weight size;
(6) with enzyme deactivation, reaction product is filtered, freezing preservation.
Claims (1)
1, the preparation method of natural active protein and peptide is characterized in that:
(1) the native protein raw material is carried out pre-treatment, described natural protein is any in animal skin, egg white, soybean or the corn; Animal skin 1-5%Na wherein
2CO
3Solution carries out degreasing, and soybean and corn are used rinsed with deionized water 3 times then with the degreasing of physical squeezing method;
(2) native protein raw material 100 weight parts after above-mentioned pre-treatment and homogenate and deionized water 100～250 weight parts add in the biochemical reactor;
(3) regulate pH=5～8 with acid or alkali, be warming up to 70～90 ℃, insulated and stirred 10～30 minutes is cooled to 30～50 ℃ or adjusting pH=7 then rapidly, is warming up to 40 ℃;
(4) add polynary compound protease 0.02～0.5 weight part, after stirring, enzyme digestion reaction 2～6 hours, described polynary compound protease by microbial protease, papoid, bromeline, stomach en-and trypsinase by 6: 1: 1: mixing in 1: 1 is formed;
(5) sampling in per 0.5 hour once adopts SDS-PAGE or HPLC/MS method to measure the molecular weight distribution of biochemical reaction product, and according to the requirement to target product molecular weight size, judgement native protein raw material is determined the enzymolysis reaction time by the degree of enzymolysis; Reaction makes enzyme deactivation after finishing at once;
(6) reaction product is filtered the freezing preservation in back.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02133462 CN1226419C (en) | 2002-07-12 | 2002-07-12 | Prepn of natural active protein and peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02133462 CN1226419C (en) | 2002-07-12 | 2002-07-12 | Prepn of natural active protein and peptide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1403583A CN1403583A (en) | 2003-03-19 |
| CN1226419C true CN1226419C (en) | 2005-11-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02133462 Expired - Lifetime CN1226419C (en) | 2002-07-12 | 2002-07-12 | Prepn of natural active protein and peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1226419C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100338224C (en) * | 2004-04-01 | 2007-09-19 | 广西大学 | Method for preparing peptide of soybean through continuous enzyme membrane reaction of soyabean protein |
| CN101263861B (en) * | 2008-05-08 | 2011-03-23 | 广西广明药业有限公司 | Preparation animal protolysate |
| CN101264316B (en) * | 2008-05-08 | 2011-06-22 | 广西广明药业有限公司 | Preparation of medicinal protolysate |
| CN101824455B (en) * | 2009-03-06 | 2013-07-17 | 香港百特有限公司 | Soy protein oligopeptide, and preparation method and use thereof |
| CN102994605B (en) * | 2012-11-28 | 2014-03-19 | 张有聪 | Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation |
| CN103305580A (en) * | 2013-07-03 | 2013-09-18 | 广州金娜宝生物科技有限公司 | Method for preparing collagen peptide from mammal skin and bone |
-
2002
- 2002-07-12 CN CN 02133462 patent/CN1226419C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1403583A (en) | 2003-03-19 |
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