CN1223344C - Parasiticides for farmed fish - Google Patents
Parasiticides for farmed fish Download PDFInfo
- Publication number
- CN1223344C CN1223344C CNB02811812XA CN02811812A CN1223344C CN 1223344 C CN1223344 C CN 1223344C CN B02811812X A CNB02811812X A CN B02811812XA CN 02811812 A CN02811812 A CN 02811812A CN 1223344 C CN1223344 C CN 1223344C
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- CN
- China
- Prior art keywords
- fish
- pyruvate
- parasite
- species
- dipping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/14—Ectoparasiticides, e.g. scabicides
Abstract
Parasiticides containing as the active ingredient at least one component selected from among methyl pyruvate, propyl pyruvate and butyl pyruvate with which parasites can be safely exterminated from farmed fish without loading any stress and the survival ratio and growth rate of the farmed fish can be improved.
Description
Technical field
The present invention relates to a kind of be used to go out remove to parasitize culture fish parasitic parasiticide on one's body.
Background technology
Parasitic parasitic breed fish such as simple eye Fugu (Fugu ocellatus), Xiao Huang bar
, Rockfiss, butterfish (Flounder, turbot), yellow bar
, growth retardation, loss of appetite life depletion then such as extra large black boat, tuna, Rockfish, tuna, filefish and Navodon septentrionalis, Anguillar japonica, salmon, Fructus Pruni pseudocerasi salmon, fragrant fish, Cyprinus carpio, coloured Cyprinus carpio, Carassius auratus, Carassius auratus, Misgurni anguillicaudati, Silurus asotus fish, tilapia, silverside, soft shell Chelomia mydas (Linnaeus)., thereby cause the reduction of commercial value.
Parasite mainly is epizoa such as Benedenia species, Neobenedenia species, Caligus species and Lepeophtheirus species or Gyrodactylus species, Heteraxine species and Heterobothrim species.Benedenia species etc. adheres to or sticks on the fish skin epithelial cell of the fish of biting or pigment cell.Heteraxine species etc. colonizes on the gill leaf of fish, and brings into play the pathological effect of direct anemia.Consequently the fish gill becomes greyish white, thereby and fish also lose appetite and become weak, when serious, cause death.
As going out, reported such as fish being carried out methods such as fresh water bath, strong brine bath and dipping except that these parasitic measures.In a single day these methods are exactly to capture fish, just place it in the stagnant water, then handle.
Owing to providing and transporting very difficulty of a large amount of fresh water, so the operability of fresh water bath method is very poor.And, owing to cause the dangerous extremely strong of fish dehydration and need a large amount of sodium chloride, so the strong brine bath method can not be used.Therefore, utilize the dipping method of formalin to be used usually.Yet in utilizing the method for formalin, to remove parasitic effect very big although go out, and the problem that exists is that formal comes the toxicity of woods very high.
Used a formalin in per 10 days with 1000 to 2000ppm concentration.This is equivalent to each fish pond fish and used an about 1000kg in, because the very poor a large amount of formalin of degradation capability are dispersed in the sea water, near marine fish the fish farm and Crustacean has been caused very big influence.Recently, such thunder bolt has taken place, a large amount of exactly formalin is accumulated in the Margarita body of being cultured, and the result causes the death of a large amount of Margaritas.At present, forbid in the fish culture industry, using formalin.
Formalin stimulates people's respiratory apparatus mucosa and have the death that can cause the people under the condition of 0.5mg in the 1L air.Therefore, formalin is a kind of breakneck chemical compound in operation.Thereby needing a kind of dipping medicine of development, this medicine is that degradation capability safe and in sea water is very strong to human body, and can not left behind.
An object of the present invention is to provide a kind of parasiticide of culturing fish, so the degradation capability of this medicine in water is very strong and can be residual very unsafe in vivo, this medicine effect is strong and can improve survival rate and the growth rate of breed fish.
Summary of the invention
In order to solve the problem of above-mentioned existence, the inventor has carried out deep research, found that by culturing fish to be immersed in the Treatment Solution that contains as the ethyl pyruvate with very strong degradation capability of active component and can to go out except that parasite.Yet, find that also when utilizing ethyl pyruvate to carry out dipping in the fish culture field, frequently breathe and bait mutually owing to anxiety in simple eye east.
Usually, when dipping is carried out in the fish culture field, consider from the angle of operability and cost, because 3000 to 5000 so a large amount of fishes are placed in the pond of containing 5 to 10 tons of water, fish is in overcrowding state and handles by adding medicine, therefore in the dipping process, excessive to the fish applied pressure.Therefore, when culturing simple eye east, owing to entering from wound, antibacterial causes disease in the body as a rule usually, so thereby need carry out tooth regularly to excise the injury that fish is subjected to as far as possible little, even can not injure too big after being snapped yet.
For this because of, the inventor studies the dipping medicine, thereby fish is moved about leisurely and do not bait mutually even this medicine also can not produce pressure in the dipping process, and find to contain at least a parasiticide that is selected from methyl pyruvate, Propyl 2-oxopropionate and the 2-Ketopropanoic acid butyl ester and can go out safely, thereby finished the present invention except that parasite and not to breed fish generation pressure as active component.
That is to say, the invention is characterized in (1) and (2).
(1) parasiticide that is used for culturing fish contains and is selected from least a as active component of methyl pyruvate, Propyl 2-oxopropionate and 2-Ketopropanoic acid butyl ester.
(2) go out except that parasitic method, be included in the processing procedure with the parasiticide that is used to culture fish described in (1) more than 50 to 1000ppm the concentration use.
The parasiticide of the application of the invention can carry out the dipping method safely and do not produce pressure to culturing fish in the dipping process.
Realize best way of the present invention
Parasiticide of the present invention can be used to any marine fish and fresh-water fishes, for example, marine fish be such as simple eye east (Fugu ocellatus), Xiao Huang bar, Rockfiss, butterfish (Flounder, turbot), yellow bar, extra large black boat, tuna,, tuna, filefish and green fin black scraper, fresh-water fishes are all eels, salmon, salmon, Fructus Pruni pseudocerasi salmon, fragrant fish, Cyprinus carpio, coloured Cyprinus carpio, Carassius auratus, Carassius auratus, Misgurni anguillicaudati, Silurus asotus fish, tilapia, silverside, soft shell Chelomia mydas (Linnaeus)..The present invention's parasitic example that removes that will go out comprises following parasite:
The parasite of yellow bar is such as Benedenia seriolae, Heteraxineheterocerca and Caligus spinosus, the parasite of Red sea crow boat is such as Bivaginatai, Philometra lateolabracis, Longicollum pagrosomi and Rhexanellaverrucosa, the parasite of black snapper is such as Cryptocarion irritans, Anoplodiscus spari and Alella macrotrachelus, the parasite of the long beak perch of striped is such as Benedenia hoshinai, the parasite of butterfish is such as the lchthyobodo species, Cryptocaryon irritans, Trichodina, the parasite in simple eye east is such as Trichodina, Gyroductylus, Heterobothrium tetrodonis, Kudoapericardialis and Boloceroides mcmurrich, the parasite of jewfish is such as Septemcapsula yasunagai, parasite be such as Microcotyle sebastisci, the parasite of leather is such as Neobenedeniella congeri, the parasite of Anguillar japonica is such as Pleistophora anguillarum, Myxidium matsui, Ichthyophthiriusmultifiliis, Pseudodactylogyrus bini, the Diplostomum species, Anguillicola globiceps, the parasite of salmon section is such as Microsporidium takedai, the Myxobolus species, Ichthyobodo necator, Chilodonella piscicola, the Trichodina species, Gyrodoctylus masu, Tetraonchus awakurai, the Acanthocephalus species, Lepeophtheirus salmonin, the Salmincola species, Argulus coregoni, Rocinela maculate, Margaritifera laevis, the parasite of fragrant fish is such as Glugea plecoglossi, Gyrodactylus japonicus, Gyrodactylus tominagai, Gyrodactylus plecoglossl, Pseudergasiluszacconis, the parasite of Cyprinus carpio and coloured Cyprinus carpio is such as Thelohanellus kitauei, Myxobolus species nakai, Chilodonella piscicola, Trichodina, Dactylogyrus extensus, Dactylogyrus minutus, Gyrodactyluskherulensis, Gyrodactylus sprostonae, Philometroides cyprini, the parasite of Carassius auratus and Carassius auratus is such as Hoferellus carassii, Ichthyophthirius multifiliis, Clinostomum complanatum, Lernaea cyprinacea, Argurus japonicus, the parasite of Misgurni anguillicaudati is such as Clinostomum complanatum, the parasite of U.S. Silurus asotus fish is such as Ichthyophthirius multifiliis, and the parasite that tilapia belongs to is such as Trichodina.
The compatibility of acetone acid esters and water is very poor, but the favorable solubility of methyl pyruvate in water.Therefore, methyl pyruvate is most preferred in three kinds of pyruvates.
On the other hand, because Propyl 2-oxopropionate and 2-Ketopropanoic acid butyl ester have very strong insecticidal action, so can obtain not only to act on by force but also the good preparation of the compatibility by methyl pyruvate is mixed with Propyl 2-oxopropionate and/or 2-Ketopropanoic acid butyl ester.
In the dipping process, the concentration of parasiticide acetone acid esters of the present invention is preferably 50 to 1000ppm.If concentration is lower than 50ppm, act on insufficient.Yet,, can culture the phenomenon that fish baits mutually if concentration surpasses 1000ppm.
Processing time is 10 minutes or longer fully.
Because parasiticide of the present invention is hydrolyzed into acetone acid and ethanol fast in water, and finally changes into carbon dioxide and water, so, even handle for a long time, culture fish and also the medicine disease can not occur.In addition, parasiticide of the present invention is a kind of environment to be had the very material of high security.
When parasiticide of the present invention periodically used for a long time repeatedly, the survival rate and the growth rate of culturing fish can be improved.
The ethyl pyruvate of pyruvate apoplexy due to endogenous wind does not have direct toxicity to fish, but is using ethyl pyruvate to carry out causing bacterial infection to cause the death of fish in the fish body owing to baiting mutually of fish makes antibacterial enter by the wound of breaking by the teeth in the process of dipping.On the contrary, methyl pyruvate, Propyl 2-oxopropionate and 2-Ketopropanoic acid butyl ester as the active component of parasiticide of the present invention can go out safely except that parasite, even in simple eye Fugu carries out the process of dipping, also do not cause this baiting, thereby can make the fish state of keeping fit of culturing.Therefore, can obtain high viability and growth rate.
Survival rate from following embodiment we can say that from security standpoint, methyl pyruvate is most preferred in three kinds of pyruvates as active component of the present invention as can be seen.
Embodiment
Following the present invention will obtain detailed explanation by embodiment and comparative example.
Embodiment 1
50 liters sea water is irritated in the polyethylene can of 100L into so that the dipping solution of different methyl pyruvates shown in the preparation table 1 and ethyl pyruvate concentration.Article ten, the parasitism simple eye Fugu of breed (body is about 10 centimetres) of about 10 kinds of epizoas (Caligus species and Neobenedenia species) is placed on wherein, and submergence 30 minutes.After the processing, ectozoic number that removes and the epizoa number that still colonizes on the simple eye Fugu obtain ectozoic going out except that rate thereby mensuration is gone out.In addition,, prepared the dipping solution of the 5000L shown in the table 1, and 3000 simple eye Fugu bodies have been placed on wherein in the fish culture field.The submergence of carrying out 30 minutes is handled, and checks the situation of simple eye Fugu.The results are shown in Table 1.
Table 1
| The composition title | Ectozoic going out except that rate (%) | Situation at dipping process median ocellus Fugu | |
| The comparative example 1 | Sea water (contrast) | 0 | Move about leisurely and do not bait mutually |
| The comparative example 2 | Methyl pyruvate 1ppm | 0 | Move about leisurely and do not bait mutually |
| Embodiment 1 | Methyl pyruvate 50ppm | 20 | Move about leisurely and do not bait mutually |
| Embodiment 2 | Methyl pyruvate 100ppm | 40 | Move about leisurely and do not bait mutually |
| Embodiment 3 | Methyl pyruvate 300ppm | 100 | Move about leisurely and do not bait mutually |
| Embodiment 4 | Methyl pyruvate 1000ppm | 100 | Move about leisurely and do not bait mutually |
| The comparative example 3 | Methyl pyruvate 2000ppm | 100 | Breathe and bait mutually |
| The comparative example 4 | Ethyl pyruvate 1ppm | 0 | Move about leisurely and do not bait mutually |
| The comparative example 5 | Ethyl pyruvate 50ppm | 30 | Breathe and bait mutually |
| The comparative example 6 | Ethyl pyruvate 100ppm | 56 | Breathe and bait mutually |
| The comparative example 7 | Ethyl pyruvate 300ppm | 100 | Breathe and bait mutually |
| The comparative example 8 | Ethyl pyruvate 1000ppm | 100 | Breathe and bait mutually |
| The comparative example 9 | Ethyl pyruvate 2000ppm | 100 | Breathe and bait mutually |
Embodiment 2
50 liters sea water is irritated in the polyethylene can of 100L into so that the dipping solution of the different methyl pyruvates shown in the preparation table 2, Propyl 2-oxopropionate and 2-Ketopropanoic acid butyl ester concentration.Article ten, the parasitism simple eye Fugu of breed (body is about 10 centimetres) of about 10 kinds of epizoas (Caligus species and Neobenedenia species) is placed on wherein, and submergence 30 minutes.After the processing, ectozoic number that removes and the epizoa number that still colonizes on the simple eye Fugu obtain ectozoic going out except that rate thereby mensuration is gone out.In addition,, prepared the dipping solution of the 5000L shown in the table 2, and 3000 simple eye Fugu bodies have been placed on wherein in the fish culture field.The submergence of carrying out 30 minutes is handled, and checks the situation of simple eye Fugu.The results are shown in Table 2.
Table 2
| The composition title | Ectozoic going out except that rate (%) | Situation at dipping process median ocellus Fugu | |
| Embodiment 5 | Propyl 2-oxopropionate 50ppm | 60 | Move about leisurely and do not bait mutually |
| Embodiment 6 | Propyl 2-oxopropionate 100ppm | 100 | Move about leisurely and do not bait mutually |
| Embodiment 7 | 2-Ketopropanoic acid butyl ester 50ppm | 100 | Move about leisurely and do not bait mutually |
| Embodiment 8 | 2-Ketopropanoic acid butyl ester 100ppm | 100 | Move about leisurely and do not bait mutually |
| Embodiment 9 | Methyl pyruvate 100 ppm+ Propyl 2-oxopropionate 100ppm | 100 | Move about leisurely and do not bait mutually |
Embodiment 3
Prepared trial zone, five fishing grounds, each district holds the simple eye Fugu of 200 about 50g of body weight, and 20 fishes of random catching come average value measured during on-test.Four districts in these test blocks, the submergence that utilizes the every fortnight of the dipping medicine shown in the table 3 (pyruvate concentration is 50ppm, and solution amount is 500L) to carry out in half a year one time 30 minutes is handled.In another test block, in half a year, do not utilize the processing of dipping medicine.After half a year, check average weight and the growth rate of 20 fishes of the survival rate of simple eye Fugu and random catching.The results are shown in Table 3.
Table 3
| The composition title | Survival rate (%) | Average weight (g) | Growth rate (%) | ||
| Initially | Finally | ||||
| The comparative example 10 | Ethyl pyruvate | 20 | 50 | 175 | 250 |
| Embodiment 10 | Methyl pyruvate | 90 | 52 | 210 | 304 |
| Embodiment 11 | Propyl 2-oxopropionate | 70 | 48 | 192 | 300 |
| Embodiment 12 | 2-Ketopropanoic acid butyl ester | 70 | 53 | 209 | 294 |
| The comparative example 11 | (district of being untreated) | 55 | 49 | 173 | 253 |
Growth rate: (final average weight-initial average weight)/initial average weight
As can be seen, when using with 2000ppm or lower concentration when being selected from least a in methyl pyruvate, Propyl 2-oxopropionate and the 2-Ketopropanoic acid butyl ester, can go out does not produce pressure to culturing fish except that parasite, and survival rate and growth rate are all very high.
Industrial applicibility
Contain and be selected from least a parasiticide in methyl pyruvate, Propyl pyruvate and the n-Butyl pyruvate can be used to go out parasite except the cultivation fish as active component.
Claims (2)
1. at least a pyruvate that is selected from methyl pyruvate, Propyl 2-oxopropionate and 2-Ketopropanoic acid butyl ester is used to produce the parasiticide of culturing fish as active component purposes.
2. the purposes of pyruvate as claimed in claim 1, the concentration of wherein handling the pyruvate of culturing fish is the 50-1000ppm weight ratio.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP179669/2001 | 2001-06-14 | ||
| JP2001179669 | 2001-06-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1514724A CN1514724A (en) | 2004-07-21 |
| CN1223344C true CN1223344C (en) | 2005-10-19 |
Family
ID=19020205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB02811812XA Expired - Fee Related CN1223344C (en) | 2001-06-14 | 2002-06-13 | Parasiticides for farmed fish |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JP4154326B2 (en) |
| CN (1) | CN1223344C (en) |
| NO (1) | NO334540B1 (en) |
| WO (1) | WO2002102366A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080300303A1 (en) | 2005-04-15 | 2008-12-04 | Klaus Huse | Substances and Pharmaceutical Compositions for the Inhibition of Glyoxalases and Their Use As Anti-Fungal Agents |
| DE102005018642B4 (en) * | 2005-04-15 | 2011-04-07 | Bio Mac- Privatinstitut für medizinische und zahnmedizinische Forschung, Entwicklung und Diagnostik GmbH | Use of compounds for the inhibition of glyoxalases |
| JP2006304750A (en) * | 2005-04-26 | 2006-11-09 | Shinei Sangyo Kk | Disease control method for freshwater fish |
| BRPI0609930A2 (en) * | 2005-05-26 | 2011-10-11 | Lilly Co Eli | use of an effective amount of spinosine, a method for improving the production of fish grown in aquaculture, and formulating |
| CN111183935A (en) * | 2018-11-15 | 2020-05-22 | 中山大学 | Method for controlling cryptocaryon irritans disease of fish by using ozone |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4024886B2 (en) * | 1996-08-29 | 2007-12-19 | 第一製網株式会社 | Parasiticides for cultured fish and methods for controlling them |
-
2002
- 2002-06-13 WO PCT/JP2002/005888 patent/WO2002102366A1/en active Application Filing
- 2002-06-13 JP JP2003504953A patent/JP4154326B2/en not_active Expired - Lifetime
- 2002-06-13 CN CNB02811812XA patent/CN1223344C/en not_active Expired - Fee Related
-
2003
- 2003-12-12 NO NO20035550A patent/NO334540B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002102366A1 (en) | 2002-12-27 |
| JPWO2002102366A1 (en) | 2004-09-30 |
| CN1514724A (en) | 2004-07-21 |
| NO334540B1 (en) | 2014-03-31 |
| NO20035550D0 (en) | 2003-12-12 |
| JP4154326B2 (en) | 2008-09-24 |
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