JP4024886B2 - Parasiticides for cultured fish and methods for controlling them - Google Patents

Parasiticides for cultured fish and methods for controlling them Download PDF

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Publication number
JP4024886B2
JP4024886B2 JP24891596A JP24891596A JP4024886B2 JP 4024886 B2 JP4024886 B2 JP 4024886B2 JP 24891596 A JP24891596 A JP 24891596A JP 24891596 A JP24891596 A JP 24891596A JP 4024886 B2 JP4024886 B2 JP 4024886B2
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Prior art keywords
acid
cultured fish
fish
seawater
controlling
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JPH1072344A (en
Inventor
一彦 奥薗
哲也 山本
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Daiichi Seimo Co Ltd
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Daiichi Seimo Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Farming Of Fish And Shellfish (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Description

【0001】
【産業上の利用分野】
本発明は養殖魚に寄生する寄生虫の駆除剤に関する。
【0002】
【従来の技術】
養殖魚のトラフグ、ハマチ、メバル、ヒラメ等に寄生虫が寄生すると成長が阻害され、食欲不振となり衰弱し商品価値が低下してしまう。ハダムシ、エラムシが主な寄生虫である。ハダムシは、魚の皮膚に吸鈎着し、魚の上皮細胞や色素胞を食害する。エラムシは魚の鰓葉に寄生し貧血という直接的病因作用で鰓が灰白色化し、食欲不振となり衰弱し、ひどい時は死に至ることもある。
【0003】
これらの寄生虫を駆除する対策としては、淡水浴、濃塩水浴、薬浴の各方法が採用されている。魚を一旦取り上げ、止水中に収容して処理する方法である。淡水浴は、大量の淡水の供給・運搬が困難なため、実質上不可能である。濃塩水浴法は、魚が脱水症状を起こす危険性が高いことと、多量の食塩が必要なためにこれも不可能である。従って現在ではホルマリンを用いた薬浴法が採用されている。しかし、ホルマリンを用いた方法では、ハダムシの駆除は可能だがエラムシの駆除ができない欠点があるとともに、定期的なホルマリン処理により魚体中に蓄積され、異臭魚の発生する問題が起こっている。
【0004】
ホルマリンは、生簀1台当り年間1000Kgも使用されており、処理後大量のホルマリンが海水中に投棄されており、近くの漁場の海産魚に対する影響も大きく、最近では多量のホルマリンが養殖中のアコヤ貝に蓄積され大量死するという事故も発生している。ホルマリンは呼吸器などの粘膜を刺激し、空気1l中0.5mgの存在で死に至ることもある。作業上も非常に危険な化合物である。従って、人体にも安全で海水中での分解性も良好、そして残留蓄積されることもなく、寄生虫を駆除できるような薬剤の開発が切望されている。
【0005】
【発明が解決しようとする課題】
本発明の目的は、海水中での分解性が良好で海産生物に残留蓄積されないような、安全で効果の高い養殖魚の寄生虫駆除剤を提供することにある。
【0006】
【課題を解決するための手段】
上記の問題を解決するために鋭意研究を進めた結果、pH3.0〜5.0に調整してなる海水中にて、養殖魚を浸漬処理することにより寄生虫を駆除できることを見いだした。pHを調整する物質としては、クエン酸・リンゴ酸・コハク酸・イタコン酸・グルコン酸乳酸・メタンスルホン酸・酒石酸・フマル酸・プロピオン酸・アジピン酸・グルタル酸・フィチン酸・レブリン酸・アスコルビン酸・エリソルビン酸・ピルビン酸・マロン酸・クロトン酸・硝酸硫酸・塩酸が使用できる。これらの物質は、食品中に含有するものや、食品添加物として認められたものであり、安全性も高く、海水中での分解性も良好である。又、pH3.0〜5.0の海水中にて養殖魚を浸漬処理する時に、水溶性高分子を含有させておくと、処理中の養殖魚の状態が非常に良好で、酸に対する負荷を軽減することができる。水溶性高分子としては、ゼラチン・キトサン・メチルセルロース・アルギン酸ソーダ・寒天・ポリアクリル酸ナトリウム・天然の粘質成分等があげられるが、特に限定されるものではない。処理時には、空気又は酸素を通気しながら行う方がより好ましい。
【0007】
試験1に示しているように、各種の酸を用いてpH3.2に調整した液で寄生虫付着トラフグを浸漬処理すると、トラフグの生存範囲内でハダムシを駆除することができる。試験3より、pHが2.5以下になると、ハダムシ駆除時間とトラフグの死ぬ時間が同一となり駆除できる時間がなくなってしまう。又、pH6.0以上になると、3時間でもハダムシを駆除することができない。従ってpH3.0〜pH5.0に調整した海水中で浸漬処理することにより、トラフグを傷めることなくハダムシを駆除することができる。
試験4より、本発明はハダムシだけでなくエラムシも駆除できることがわかる。又、試験5に示しているように、水溶性高分子を酸と併用すると、処理中の魚の状態も良好で、処理後海水に戻した時もすぐに泳ぎだす程、魚への安全性が高くなる。
【0008】
(試験1)
100lのポリ容器に80lの海水を入れ、各酸物質をpH3.2になるように添加混溶した後、ハダムシが寄生している養殖トラフグを2匹投入し、ハダムシがトラフグから脱落し死滅する時間を調査した。
又、一定時間処理した後、海水に戻し48時間後の状態を観察しトラフグへの影響を調査した。処理時の水温は25℃で行った。結果は表1に示す。
【0009】
【表1】

Figure 0004024886
【0010】
(試験2)
各酸物質を2種類使用し、pH3.0〜5.0の間に調整し、その他は試験例1と同様にしてハダムシ駆除試験を行った。結果は表2に示す。
【0011】
【表2】
Figure 0004024886
【0012】
(試験3)
プロピオン酸を用いてpH2.5〜6.0の間に調整し、その他は試験1と同様にしてハダムシ駆除試験を行った。結果は表3に示す。
【0013】
【表3】
Figure 0004024886
【0014】
(試験4)
100lのポリ容器に80lの海水を入れ、プロピオン酸を添加して、pH3.5及び4.0に調整した後、エラムシの寄生したトラフグからエラムシを採取し、浸漬処理し死滅する時間を調査した。結果は表4に示す。
【0015】
【表4】
Figure 0004024886
【0016】
(試験5)
100lのポリ容器に80lの海水を入れ、プロピオン酸を添加してpH4.5に調整した後、ゼラチンを0.01w/v%又は0.1w/v%になるように添加溶解し処理液2検体を調整した。次に、プロピオン酸を添加しpH4.5にしたものを調整し、比較試験区とした。これらの3処理区に、ハダムシが寄生している養殖トラフグを2匹投入し、ハダムシの駆除時間と、トラフグの状態を観察した。処理時の水温は25℃で行った。結果は表5に示す。
【0017】
【表5】
Figure 0004024886
【0018】
【発明の効果】
クエン酸・リンゴ酸・コハク酸・イタコン酸・プロピオン酸等の特定の酸によりpH3.0〜5.0に調整してなる海水中にて養殖魚を浸漬処理することにより、寄生虫を駆除することができる。[0001]
[Industrial application fields]
The present invention relates to a parasite-controlling agent parasitic on farmed fish.
[0002]
[Prior art]
If parasites infest the farmed fish, such as trough puffer fish, yellowtail, sea bream, and Japanese flounder, the growth is inhibited, the appetite is weakened, and the product value is reduced. Hadamushi and aphids are the main parasites. The damselfly adsorbs on the skin of the fish and eats the fish epithelial cells and pigment vesicles. The aphids parasitize the fish leaves, and the angiogenesis is a direct etiological effect that turns the sharks off white, causing loss of appetite and debilitating.
[0003]
As countermeasures against these parasites, fresh water bath, concentrated salt water bath, and medicine bath are employed. This is a method of picking up fish once and storing it in still water. A fresh water bath is virtually impossible because it is difficult to supply and transport a large amount of fresh water. Concentrated salt bathing is not possible due to the high risk of fish dehydration and the need for large amounts of salt. Therefore, the chemical bath method using formalin is currently employed. However, the method using formalin has the disadvantages that it is possible to control hard beetles but cannot control the aphids, and there is a problem in that it is accumulated in the fish body by regular formalin treatment to generate off-flavor fish.
[0004]
Formalin is used at an annual rate of 1000 kg per ginger, and after treatment, a large amount of formalin is dumped in the seawater, which has a great impact on marine fish at nearby fishing grounds. Recently, a large amount of formalin is being cultivated in Akoya. There have also been accidents involving the accumulation of shellfish and death. Formalin stimulates mucous membranes such as the respiratory tract and may be fatal in the presence of 0.5 mg in 1 liter of air. It is a very dangerous compound for work. Accordingly, there is an urgent need to develop a drug that is safe for the human body, has good degradability in seawater, and does not accumulate and can be used to combat parasites.
[0005]
[Problems to be solved by the invention]
An object of the present invention is to provide a safe and highly effective parasite control agent for cultured fish that has good degradability in seawater and does not accumulate in sea products.
[0006]
[Means for Solving the Problems]
As a result of diligent research to solve the above problems, it has been found that parasites can be controlled by immersing cultured fish in seawater adjusted to pH 3.0 to 5.0. Substances that adjust pH include citric acid, malic acid, succinic acid, itaconic acid, gluconic acid , lactic acid, methanesulfonic acid, tartaric acid, fumaric acid, propionic acid, adipic acid, glutaric acid, phytic acid, levulinic acid, ascorbine acid erythorbic acid, pyruvic acid, malonic acid, crotonic acid, nitric acid, and sulfuric acid and hydrochloric acid, can be used. These substances are those contained in foods and recognized as food additives, and have high safety and good degradability in seawater. In addition, when immersing cultured fish in seawater with a pH of 3.0 to 5.0, if the water-soluble polymer is included, the state of the cultured fish being treated is very good and the load on acid is reduced. can do. Examples of the water-soluble polymer include gelatin, chitosan, methylcellulose, sodium alginate, agar, sodium polyacrylate, natural viscous components, and the like, but are not particularly limited. It is more preferable to carry out the treatment while ventilating air or oxygen.
[0007]
As shown in Test 1, when a parasite-attached trough puffer is dipped in a solution adjusted to pH 3.2 using various acids, it is possible to eliminate the beetle within the trough puffer's survival range. From the test 3, when the pH is 2.5 or less, the time for removing the dam and the time for trough puffing is the same, and there is no time for the removal. Moreover, if it becomes pH 6.0 or more, it will be impossible to get rid of it. Therefore, by carrying out the immersion treatment in seawater adjusted to pH 3.0 to pH 5.0, it is possible to remove the beetle without damaging the trough.
From Test 4, it can be seen that the present invention can eliminate not only hard beetles but also aphids. In addition, as shown in Test 5, when a water-soluble polymer is used in combination with an acid, the condition of the fish being treated is good, and the fish is safe enough to swim immediately when returned to seawater after treatment. Get higher.
[0008]
(Test 1)
Add 80 liters of seawater to a 100 liter plastic container, add and mix each acid substance to pH 3.2, and then add 2 cultured trough puffers that are parasitized. Investigate time.
Moreover, after processing for a fixed time, it returned to seawater and the state 48 hours after was observed, and the influence on a trough puffer was investigated. The water temperature during the treatment was 25 ° C. The results are shown in Table 1.
[0009]
[Table 1]
Figure 0004024886
[0010]
(Test 2)
Two kinds of each acid substance were used, adjusted to pH 3.0 to 5.0, and others were subjected to the Hadamushi control test in the same manner as in Test Example 1. The results are shown in Table 2.
[0011]
[Table 2]
Figure 0004024886
[0012]
(Test 3)
The control was carried out in the same manner as in Test 1 except that the pH was adjusted between 2.5 and 6.0 using propionic acid. The results are shown in Table 3.
[0013]
[Table 3]
Figure 0004024886
[0014]
(Test 4)
After adding 80 l of seawater to a 100 l plastic container and adding propionic acid to adjust the pH to 3.5 and 4.0, the aphids were collected from trough pufferfish that were parasitized by the aphids, and the time to kill them by immersion treatment was investigated. . The results are shown in Table 4.
[0015]
[Table 4]
Figure 0004024886
[0016]
(Test 5)
Add 80 l of seawater to a 100 l plastic container, add propionic acid to adjust the pH to 4.5, add and dissolve gelatin to 0.01 w / v% or 0.1 w / v% The specimen was adjusted. Next, what adjusted the pH to 4.5 by adding propionic acid was prepared, and it was set as the comparative test group. Two cultured trough puffer fishes parasitized with stag beetles were introduced into these three treatment areas, and the control time of the stag beetles and the state of tiger puffers were observed. The water temperature during the treatment was 25 ° C. The results are shown in Table 5.
[0017]
[Table 5]
Figure 0004024886
[0018]
【The invention's effect】
Exterminate parasites by immersing cultured fish in seawater adjusted to pH 3.0-5.0 with specific acids such as citric acid, malic acid, succinic acid, itaconic acid, propionic acid be able to.

Claims (3)

クエン酸・リンゴ酸・コハク酸・イタコン酸・グルコン酸乳酸・メタンスルホン酸・酒石酸・フマル酸・プロピオン酸・アジピン酸・グルタル酸・フィチン酸・レブリン酸・アスコルビン酸・エリソルビン酸・ピルビン酸・マロン酸・クロトン酸・硝酸硫酸・塩酸の中の1種以上を含有し、且つ、処理時のpHが3.0〜5.0に調整してなることを特徴とする養殖魚の寄生虫駆除剤。Citric acid, malic acid, succinic acid, itaconic acid, gluconic acid , lactic acid, methanesulfonic acid, tartaric acid, fumaric acid, propionic acid, adipic acid, glutaric acid, phytic acid, levulinic acid, ascorbic acid, erythorbic acid, pyruvic acid, Parasite control of cultured fish characterized by containing at least one of malonic acid, crotonic acid, nitric acid , sulfuric acid and hydrochloric acid , and adjusting the pH during treatment to 3.0 to 5.0 Agent. 水溶性高分子を含有することを特徴とする請求項1記載の養殖魚の寄生虫駆除剤。  The parasite-controlling agent for cultured fish according to claim 1, comprising a water-soluble polymer. 請求項1又は2記載の寄生虫駆除剤を水又は海水で希釈した処理液に、寄生虫の寄生した養殖魚を浸漬処理することを特徴とする養殖魚の寄生虫駆除方法。A method for controlling parasites of cultured fish, comprising immersing the cultured fish infested with parasites in a treatment solution obtained by diluting the parasite control agent according to claim 1 or 2 with water or seawater.
JP24891596A 1996-08-29 1996-08-29 Parasiticides for cultured fish and methods for controlling them Expired - Fee Related JP4024886B2 (en)

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JP2002338538A (en) * 2001-05-21 2002-11-27 Takeda Schering-Plough Animal Health Kk New sulfonamide derivative and antiparasitic agent containing the same and used for fishes
JP4154326B2 (en) * 2001-06-14 2008-09-24 第一製網株式会社 Parasiticides for cultured fish
JP4976006B2 (en) * 2005-03-29 2012-07-18 日本水産株式会社 Parasitic disease prevention and treatment method for marine fish
WO2009022424A1 (en) * 2007-08-16 2009-02-19 National University Corporation Kochi University Agent for exterminating pathogenic bacteria in fishes and method of exterminating the same
JP5270135B2 (en) * 2007-11-16 2013-08-21 タテホ化学工業株式会社 Fish disease treatment

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