CN1220497C - Antivirus antioxidizing active extract of proplis and its molecular inclusion compound - Google Patents
Antivirus antioxidizing active extract of proplis and its molecular inclusion compound Download PDFInfo
- Publication number
- CN1220497C CN1220497C CNB011417633A CN01141763A CN1220497C CN 1220497 C CN1220497 C CN 1220497C CN B011417633 A CNB011417633 A CN B011417633A CN 01141763 A CN01141763 A CN 01141763A CN 1220497 C CN1220497 C CN 1220497C
- Authority
- CN
- China
- Prior art keywords
- propolis
- powder
- deleading
- purification
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000284 extract Substances 0.000 title claims abstract description 52
- 230000003064 anti-oxidating effect Effects 0.000 title abstract description 23
- 230000002155 anti-virotic effect Effects 0.000 title abstract description 21
- 150000001875 compounds Chemical class 0.000 title description 4
- 229940069949 propolis Drugs 0.000 claims abstract description 306
- 241000241413 Propolis Species 0.000 claims abstract description 301
- 239000000843 powder Substances 0.000 claims abstract description 146
- 239000002994 raw material Substances 0.000 claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 42
- 238000001914 filtration Methods 0.000 claims abstract description 24
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 23
- 238000001035 drying Methods 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 71
- 238000005516 engineering process Methods 0.000 claims description 27
- 239000002904 solvent Substances 0.000 claims description 15
- 239000011347 resin Substances 0.000 claims description 13
- 229920005989 resin Polymers 0.000 claims description 13
- 238000010521 absorption reaction Methods 0.000 claims description 11
- 239000003463 adsorbent Substances 0.000 claims description 5
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 4
- 239000003456 ion exchange resin Substances 0.000 claims description 4
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000002481 ethanol extraction Methods 0.000 claims 2
- 239000004480 active ingredient Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 45
- 229930003944 flavone Natural products 0.000 abstract description 40
- 235000011949 flavones Nutrition 0.000 abstract description 40
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 abstract description 36
- 150000002212 flavone derivatives Chemical class 0.000 abstract description 36
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 abstract description 36
- 238000002360 preparation method Methods 0.000 abstract description 16
- 238000000605 extraction Methods 0.000 abstract description 13
- 239000002105 nanoparticle Substances 0.000 abstract description 10
- 239000003094 microcapsule Substances 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 6
- -1 microballoons Substances 0.000 abstract description 6
- 235000013402 health food Nutrition 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 abstract description 3
- 230000008685 targeting Effects 0.000 abstract description 2
- 238000000746 purification Methods 0.000 description 61
- 230000036541 health Effects 0.000 description 48
- 239000000047 product Substances 0.000 description 46
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 36
- 239000000203 mixture Substances 0.000 description 32
- 241000256844 Apis mellifera Species 0.000 description 28
- RTIXKCRFFJGDFG-UHFFFAOYSA-N Chrysin Natural products C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=CC=C1 RTIXKCRFFJGDFG-UHFFFAOYSA-N 0.000 description 28
- 239000002202 Polyethylene glycol Substances 0.000 description 25
- 229920001223 polyethylene glycol Polymers 0.000 description 25
- 239000007788 liquid Substances 0.000 description 24
- VCCRNZQBSJXYJD-UHFFFAOYSA-N galangin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=CC=C1 VCCRNZQBSJXYJD-UHFFFAOYSA-N 0.000 description 22
- 230000000694 effects Effects 0.000 description 21
- 229960004756 ethanol Drugs 0.000 description 21
- 239000003292 glue Substances 0.000 description 19
- 239000002775 capsule Substances 0.000 description 18
- 229920000858 Cyclodextrin Polymers 0.000 description 15
- 241000222336 Ganoderma Species 0.000 description 15
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 14
- 239000000463 material Substances 0.000 description 13
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 13
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 12
- 239000012535 impurity Substances 0.000 description 12
- 241000256836 Apis Species 0.000 description 11
- 229910052785 arsenic Inorganic materials 0.000 description 11
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 11
- 230000001413 cellular effect Effects 0.000 description 11
- 238000000586 desensitisation Methods 0.000 description 11
- NYCXYKOXLNBYID-UHFFFAOYSA-N 5,7-Dihydroxychromone Natural products O1C=CC(=O)C=2C1=CC(O)=CC=2O NYCXYKOXLNBYID-UHFFFAOYSA-N 0.000 description 10
- 208000024172 Cardiovascular disease Diseases 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 10
- 206010020772 Hypertension Diseases 0.000 description 10
- 201000004681 Psoriasis Diseases 0.000 description 10
- 208000007107 Stomach Ulcer Diseases 0.000 description 10
- 239000007766 cera flava Substances 0.000 description 10
- 208000026106 cerebrovascular disease Diseases 0.000 description 10
- 235000015838 chrysin Nutrition 0.000 description 10
- 229940043370 chrysin Drugs 0.000 description 10
- 206010012601 diabetes mellitus Diseases 0.000 description 10
- 208000000718 duodenal ulcer Diseases 0.000 description 10
- 230000002526 effect on cardiovascular system Effects 0.000 description 10
- CIPSYTVGZURWPT-UHFFFAOYSA-N galangin Natural products OC1=C(Oc2cc(O)c(O)cc2C1=O)c3ccccc3 CIPSYTVGZURWPT-UHFFFAOYSA-N 0.000 description 10
- 208000019622 heart disease Diseases 0.000 description 10
- 229940023488 pill Drugs 0.000 description 10
- 239000006187 pill Substances 0.000 description 10
- SUYJZKRQHBQNCA-UHFFFAOYSA-N pinobanksin Natural products O1C2=CC(O)=CC(O)=C2C(=O)C(O)C1C1=CC=CC=C1 SUYJZKRQHBQNCA-UHFFFAOYSA-N 0.000 description 10
- 239000011669 selenium Substances 0.000 description 10
- 208000007882 Gastritis Diseases 0.000 description 9
- 206010019799 Hepatitis viral Diseases 0.000 description 9
- 208000031226 Hyperlipidaemia Diseases 0.000 description 9
- 208000029078 coronary artery disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 201000005917 gastric ulcer Diseases 0.000 description 9
- 206010022000 influenza Diseases 0.000 description 9
- 229910052711 selenium Inorganic materials 0.000 description 9
- 201000001862 viral hepatitis Diseases 0.000 description 9
- 229940038481 bee pollen Drugs 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 239000003993 organochlorine pesticide Substances 0.000 description 8
- 239000007901 soft capsule Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- QAIPRVGONGVQAS-DUXPYHPUSA-N trans-caffeic acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-DUXPYHPUSA-N 0.000 description 8
- 239000013543 active substance Substances 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 7
- 239000006196 drop Substances 0.000 description 7
- URFCJEUYXNAHFI-ZDUSSCGKSA-N pinocembrin Chemical compound C1([C@@H]2CC(=O)C3=C(O)C=C(C=C3O2)O)=CC=CC=C1 URFCJEUYXNAHFI-ZDUSSCGKSA-N 0.000 description 7
- 230000002265 prevention Effects 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- FGUBFGWYEYFGRK-HNNXBMFYSA-N Pinocembrin Natural products Cc1cc(C)c2C(=O)C[C@H](Oc2c1)c3ccccc3 FGUBFGWYEYFGRK-HNNXBMFYSA-N 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 239000004005 microsphere Substances 0.000 description 6
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 6
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 108010010803 Gelatin Proteins 0.000 description 5
- 229910004298 SiO 2 Inorganic materials 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 239000008273 gelatin Substances 0.000 description 5
- 229920000159 gelatin Polymers 0.000 description 5
- 235000019322 gelatine Nutrition 0.000 description 5
- 235000011852 gelatine desserts Nutrition 0.000 description 5
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 5
- 239000003826 tablet Substances 0.000 description 5
- ACEAELOMUCBPJP-UHFFFAOYSA-N (E)-3,4,5-trihydroxycinnamic acid Natural products OC(=O)C=CC1=CC(O)=C(O)C(O)=C1 ACEAELOMUCBPJP-UHFFFAOYSA-N 0.000 description 4
- OIALAIQRYISUEV-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-hydroxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]e Polymers CCCCCCCCCCCCCCCCCC(=O)OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO OIALAIQRYISUEV-UHFFFAOYSA-N 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 235000004883 caffeic acid Nutrition 0.000 description 4
- 229940074360 caffeic acid Drugs 0.000 description 4
- 229940112822 chewing gum Drugs 0.000 description 4
- 235000015218 chewing gum Nutrition 0.000 description 4
- QAIPRVGONGVQAS-UHFFFAOYSA-N cis-caffeic acid Natural products OC(=O)C=CC1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-UHFFFAOYSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 150000002213 flavones Chemical class 0.000 description 4
- 229920001451 polypropylene glycol Polymers 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000000194 supercritical-fluid extraction Methods 0.000 description 4
- 208000002874 Acne Vulgaris Diseases 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 206010000496 acne Diseases 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 239000000443 aerosol Substances 0.000 description 3
- 235000013877 carbamide Nutrition 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 3
- 230000007661 gastrointestinal function Effects 0.000 description 3
- 235000015110 jellies Nutrition 0.000 description 3
- 239000008274 jelly Substances 0.000 description 3
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 3
- 210000000867 larynx Anatomy 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 235000007173 Abies balsamea Nutrition 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000035619 Back crushing Diseases 0.000 description 2
- 239000004857 Balsam Substances 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000700199 Cavia porcellus Species 0.000 description 2
- 206010008570 Chloasma Diseases 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 244000236655 Diospyros kaki Species 0.000 description 2
- 235000008597 Diospyros kaki Nutrition 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 240000008397 Ganoderma lucidum Species 0.000 description 2
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 2
- 244000018716 Impatiens biflora Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- GQODBWLKUWYOFX-UHFFFAOYSA-N Isorhamnetin Natural products C1=C(O)C(C)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 GQODBWLKUWYOFX-UHFFFAOYSA-N 0.000 description 2
- 206010067125 Liver injury Diseases 0.000 description 2
- 208000003351 Melanosis Diseases 0.000 description 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 2
- IKMDFBPHZNJCSN-UHFFFAOYSA-N Myricetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC(O)=C(O)C(O)=C1 IKMDFBPHZNJCSN-UHFFFAOYSA-N 0.000 description 2
- 241001597008 Nomeidae Species 0.000 description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000036783 anaphylactic response Effects 0.000 description 2
- 208000003455 anaphylaxis Diseases 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- XADJWCRESPGUTB-UHFFFAOYSA-N apigenin Natural products C1=CC(O)=CC=C1C1=CC(=O)C2=CC(O)=C(O)C=C2O1 XADJWCRESPGUTB-UHFFFAOYSA-N 0.000 description 2
- 235000008714 apigenin Nutrition 0.000 description 2
- KZNIFHPLKGYRTM-UHFFFAOYSA-N apigenin Chemical compound C1=CC(O)=CC=C1C1=CC(=O)C2=C(O)C=C(O)C=C2O1 KZNIFHPLKGYRTM-UHFFFAOYSA-N 0.000 description 2
- 229940117893 apigenin Drugs 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- BEWYHVAWEKZDPP-UHFFFAOYSA-N bornane Chemical compound C1CC2(C)CCC1C2(C)C BEWYHVAWEKZDPP-UHFFFAOYSA-N 0.000 description 2
- 229940046011 buccal tablet Drugs 0.000 description 2
- 239000006189 buccal tablet Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 239000007938 effervescent tablet Substances 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 210000001156 gastric mucosa Anatomy 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 231100000753 hepatic injury Toxicity 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 235000008800 isorhamnetin Nutrition 0.000 description 2
- IZQSVPBOUDKVDZ-UHFFFAOYSA-N isorhamnetin Chemical compound C1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 IZQSVPBOUDKVDZ-UHFFFAOYSA-N 0.000 description 2
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- 150000002596 lactones Chemical class 0.000 description 2
- 229940040145 liniment Drugs 0.000 description 2
- 239000000865 liniment Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- PCOBUQBNVYZTBU-UHFFFAOYSA-N myricetin Natural products OC1=C(O)C(O)=CC(C=2OC3=CC(O)=C(O)C(O)=C3C(=O)C=2)=C1 PCOBUQBNVYZTBU-UHFFFAOYSA-N 0.000 description 2
- 235000007743 myricetin Nutrition 0.000 description 2
- 229940116852 myricetin Drugs 0.000 description 2
- 239000002088 nanocapsule Substances 0.000 description 2
- 229920001206 natural gum Polymers 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 235000005875 quercetin Nutrition 0.000 description 2
- 229960001285 quercetin Drugs 0.000 description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- 230000004223 radioprotective effect Effects 0.000 description 2
- 230000003578 releasing effect Effects 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 235000017709 saponins Nutrition 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical class C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- RBNPOMFGQQGHHO-UHFFFAOYSA-N -2,3-Dihydroxypropanoic acid Natural products OCC(O)C(O)=O RBNPOMFGQQGHHO-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 241001502050 Acis Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 206010003399 Arthropod bite Diseases 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 206010005963 Bone formation increased Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- RBNPOMFGQQGHHO-UWTATZPHSA-N D-glyceric acid Chemical compound OC[C@@H](O)C(O)=O RBNPOMFGQQGHHO-UWTATZPHSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 206010048768 Dermatosis Diseases 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- CTKXFMQHOOWWEB-UHFFFAOYSA-N Ethylene oxide/propylene oxide copolymer Chemical compound CCCOC(C)COCCO CTKXFMQHOOWWEB-UHFFFAOYSA-N 0.000 description 1
- 239000005770 Eugenol Substances 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 239000009429 Ginkgo biloba extract Substances 0.000 description 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
- XETQTCAMTVHYPO-UHFFFAOYSA-N Isocamphan von ungewisser Konfiguration Natural products C1CC2C(C)(C)C(C)C1C2 XETQTCAMTVHYPO-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920003091 Methocel™ Polymers 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 208000010195 Onychomycosis Diseases 0.000 description 1
- 208000025157 Oral disease Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 240000004371 Panax ginseng Species 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 240000007164 Salvia officinalis Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241000934136 Verruca Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 206010047601 Vitamin B1 deficiency Diseases 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- BAPJBEWLBFYGME-UHFFFAOYSA-N acrylic acid methyl ester Natural products COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 230000000146 antalgic effect Effects 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000181 anti-adherent effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 208000002894 beriberi Diseases 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229930006742 bornane Natural products 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229960003563 calcium carbonate Drugs 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 208000021735 chronic enteritis Diseases 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003221 ear drop Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229960002217 eugenol Drugs 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 description 1
- 229940080345 gamma-cyclodextrin Drugs 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000007903 gelatin capsule Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229940071826 hydroxyethyl cellulose Drugs 0.000 description 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QRWOVIRDHQJFDB-UHFFFAOYSA-N isobutyl cyanoacrylate Chemical compound CC(C)COC(=O)C(=C)C#N QRWOVIRDHQJFDB-UHFFFAOYSA-N 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000021056 liquid food Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000002690 local anesthesia Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 229960000816 magnesium hydroxide Drugs 0.000 description 1
- 235000012254 magnesium hydroxide Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229920003145 methacrylic acid copolymer Polymers 0.000 description 1
- 229940117841 methacrylic acid copolymer Drugs 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000007708 morin Nutrition 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000008601 oleoresin Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- NOOLISFMXDJSKH-UHFFFAOYSA-N p-menthan-3-ol Chemical compound CC(C)C1CCC(C)CC1O NOOLISFMXDJSKH-UHFFFAOYSA-N 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 229940044519 poloxamer 188 Drugs 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000003405 preventing effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000021568 protein beverage Nutrition 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000005412 red sage Nutrition 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 201000003068 rheumatic fever Diseases 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 125000002298 terpene group Chemical group 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 201000005882 tinea unguium Diseases 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 235000015192 vegetable juice Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
Landscapes
- Jellies, Jams, And Syrups (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention discloses an antivirus antioxidizing active extract of propolis, purified lead removing propolis powder, and a manufacture method thereof. Through the improvements of the technical skill of raw material pretreatment, dissolvent extraction, filtration and drying in the process of purifying propolis, the purity of the antivirus antioxidizing active extract of propolis and the total flavone content of propolis are improved, and the novel technical skill of five kinds of removal, such as propolis hyposensitization, heavy metal lead removal, etc., is innovated. The present invention also discloses a group of propolis food and health food, and particularly relates to novel preparations, which has a targeting property and a slow control release property, such as molecular clathrate, micro capsules, microballoons, nanoparticles, etc., and manufacture methods thereof.
Description
Technical field
The present invention relates to the propolis health-care products that a kind of propolis extracting method and this method products therefrom thereof make.
Background technology
The source of propolis, characteristics, the biological property that Main Ingredients and Appearance and Apis thereof are gathered propolis all has introduction in following known technology: (Shanxi science tech publishing house publishes " propolis ", pillars of a house chief editor, January in 1999 the 1st edition, the 1-19 page or leaf), " the surprising drug effect of propolis " (Taiwan justice publishing house, July in 1992 the 1st edition, numerous professor Tai Lang chief editor under the clear and medical university's wood of Japan, the Liu Xueqing translation, the 20-42 page or leaf), (Paris, FRA Librairie Maloine S.A.Editeur publishes " Propolisin Natural Therpeutics ", nineteen eighty-three the 2nd revised edition, the 20-28 page or leaf) and " プ ロ Port リ ス そ drug effect The visit Ru " (Chinese " drug efficacy study of propolis " by name, リ ョ Application society publishes, March in 1994 the 1st edition, Song Yezhe also writes).
Propolis (Propolis) is that Apis is gathered extraneous plant sprout bud and secreted natural gum (Gum), resin (Resin), balsam (Balsam) and the oleoresin resinoids such as (Oil-resin) in trunk wound, and sneaks into Apis salivary gland secretion (sialic acid Saliva acid and ptyalin Saliva enzyme) and the formed a kind of yellowish-brown jelly of Cera Flava (Wax).
Propolis derives from the secretions of plant.The first is after plant comes to harm or poisons, just secrete a kind of yellowish-brown jelly from epidermis, this jelly is the pathological product of plant, mainly be to be used for protective plant to exempt from injury, wherein mainly be the bigger complicated pluralism phenolic compound (Phytoncide) of natural gum, resin, volatile oil, tannin (Tannin) and molecule, these materials can be that insoluble matter is used to suppress the infringement to plant such as antibacterial and virus with protein bound; It two is plant tree bud, leaves and the normal resinae secretions of trunk epidermis.The physiology product that these two kinds of secretions all are plant metabolisms has the complex physical effect, is that plant adapts to natural biological nature.
Owing to contain many aromatics volatile matters in the propolis and have the polyphenols of maximum absorption band at ultraviolet region, therefore, Apis is gathered propolis and is not only in order to fill up slit in the Nidus Vespae, prevent the invasion of disease harmful animal, and be the stimulant (attractant) of a kind of bee gathering and visual activity, be that Apis adapts to natural a kind of biological nature.
The inventor concentrates on studies for many years, test and analytical test are learnt, are to contain multiple active substance composition with special pharmacological drug action in the bee glue purifying of raw material production with Chinese propolis: wherein mainly comprise propolis flavone (Propolis Flavonoids), propolis lignanoid (Propolis Lignin), propolis coumarin (PropolisCoumarins), propolis lactone (Propolis Lactone), propolis anthraquinone (Propolis Anthraguinone), propolis stilbene class (Propolis Stilbene), propolis Saponin (Propolis Saponins), propolis volatile oil (Propolis Oils), propolis organic acid (Propolis Organic acis) and propolis terpenoid nearly thousand kinds of active substance compositions of ten big classes such as (Propolis Terpenoides).Be described as by medical expert " natural plants explosive component treasure-house ", claim that Apis is " magical medical expert ".
The pharmacology and the drug action of propolis mainly contain: 1. antibacterial action.The propolis tool is wanted the antibacterial and bactericidal action of broad-spectrum, and antibiotics is had obvious synergistic effect; 2. blood circulation promoting and blood stasis dispelling, anti-inflammatory and antalgic, antiulcer action; 3. antioxidation and antifatigue effect; 4. antiviral, protect the liver and improve the gastrointestinal function effect; 5. resisiting influenza virus effect; 6. hypoglycemic activity; 7. vessel softening and effect for reducing blood fat; 8. hypotensive activity; 9. immunostimulant, slow down aging, microcirculation improvement and antitumaous effect; 10. local anesthesia and promotion tissue regeneration effect.Propolis has peculiar prevention and therapeutical effect to human numerous disease, can be used for treatment: 1. dermatosis: eczema, acne, verruca plana, psoriasis and beriberi etc.; 2. ear,nose ﹠ throat disease; 3. oral disease; 4. internal disease: gastric and duodenal ulcers disease, chronic enteritis, hepatitis, respiratory organ disease, hyperlipemia and blood high viscosity syndrome; 5. prevent and treat diabetes, hypertension and heart disease, cardiovascular and cerebrovascular disease; 6. surgical diseases: sterilization, pain relieving, treatment are burnt, chronic ulcer, hyperosteogeny, osteoporosis, rheumatic and atrophic arthritis and damage, anus etc.; 7. other disease: tinea unguium, gynaecopathia, ophthalmic diseases, tuberculosis; 8. antitumor is as prevention and auxiliary treatment nasopharyngeal carcinoma, breast carcinoma and leukemia etc.
In the active substance composition of the above-mentioned multiple special pharmacological drug action that contains, that propolis the most important thing is is antibiotic, antiviral, biological antioxidant and anti-tumor activity composition.
The propolis active substance composition that contains 35-45% in the raw material propolis approximately is a bee glue purifying, other 55-65% is an insoluble impurities, mainly contains 1. Cera Flava, ethanol insoluble resin and some insoluble impuritiess such as wood flour, residual broken Apis body, wire gauze disintegrating slag, iron nail, fine earth slag, fine sand and crushed stone etc.; 2. the nonactive composition of hot ethanol soluble resin class; 3. heavy metal pollution (as lead, hydrargyrum and arsenic etc.); 4. agricultural residual pollution; 5. main sensitizer.
At present, it mostly is edible ethanol and the heating extraction of taking the 70-90% variable concentrations that the purification of propolis is both at home and abroad produced, filter method is simple coarse also, make and contain insoluble impurities and harmful heavy metal etc. in the final propolis extract, thereby make effective component content contained in the propolis on the low side relatively.Therefore, existing propolis extraction process still has many weak points:
(1) extracts temperature drift: extract temperature generally at 55-65 ℃, dissolved the extracting of the nonactive composition of resinae of a small amount of Cera Flava and some solubilities will be arranged like this; 2. filtering accuracy is low: filter method generally is to filter with 200 order metal screens; Or earlier with 80 order gauze coarse filtration, reuse 300 order gauze filter pressings; 3. it is low to extract solvent strength: the edible ethanol that mainly is to use the 70-90% variable concentrations; 4. the ratio of extracting solvent and propolis raw material is little: generally be 1.5 to 1; 5. still contain a small amount of Cera Flava and insoluble impurities; 6. contain soluble impurity; 7. heavy metal and agricultural residual pollution are arranged; 8. unresolved propolis allergic problem; 9. thoroughly do not solve the agricultural technology problem of full component water-soluble bee glue: the preparation method of water-soluble bee glue is directly to carry out processing with the raw material propolis at present.Should be purification deleading, desensitization and the purified technology production technology problem thereof that solves the raw material propolis earlier, on the basis of bee glue purifying, the reuse dissolution with solvents prepares water-soluble bee glue then.
Ah he's wound of Amada Co., Ltd.'s woods protobiochemistry institute waits at Chinese patents CN1194967A " has the water miscible propolis extract of improvement ": it is characterized in that preparation propolis extractive with organic solvent earlier, extract with the solvent that contains alkali or basic anhydride of PH between 5-7 then, propolis composition in the prepared propolis water solubility extract remains the water-soluble bee glue extract of propolis-containing part component, is not full component water-soluble bee glue extract.Name is called to have disclosed in No. 00105486.4 Chinese patent application of " processing art for extracting propolis and removing lead from it " a kind ofly " to be pulverized propolis after below-4 ℃ freezing 12 hours through liquid nitrogen cryogenics; The variable concentrations edible ethanol is merceration repeatedly; Filtering lead is got rid of in freezing sedimentation under the critical state; Binodal energy vacuum and low temperature concentrating under reduced pressure; A kind of processing art for extracting propolis and removing lead from it that the method that alcohol distillation column distillation recovery alcohol in high concentration utilizes is again finished." not only deleading is incomplete in this patent application, and mentions for not giving such as the removal of hydrargyrum, arsenic one class poisonous metal, do not give equally for the removal of the removal of residual pesticide and heat-sensitive materials relating to.
Therefore, existing propolis purifying technique is anxious waits to improve.
In sum, the application of propolis at first will solve the purification of propolis raw material and remove heavy metal lead, desensitization etc., the propolis Patent document data of nearly by retrieval 40 years (after 1962) domestic and international more or less a hundred countries, in conclusive evidence propolis purification technique and the production technology, also do not solve simultaneously and remove five purification technique that takes off and production technologies such as heavy metal lead and desensitization.
Summary of the invention
---the invention provides a kind of preparation method of pure deleading propolis powder and the health preparation of the propolis powder system that makes by this method.It has improved pre-treatment, solvent extraction, filtration and the drying technique of raw material in the propolis purification process preparation method provided by the invention, thereby has improved the purity of propolis antivirus antioxidizing active extract and the content of propolis total flavones; And provide in the propolis purification process purify Cera Flava and insoluble impurities, remove organochlorine pesticide residual, desensitize, remove such as a class heavy metal such as lead and hydrargyrum and the nonactive composition of resinae etc. five that removes some solubilities and take off technology.
---in the manufacture method of purification deleading propolis powder provided by the invention, comprising: 1. the raw material propolis is being higher than pre-freeze under-4 ℃-0 ℃ the condition; 2. the raw material propolis after the pre-freeze is crushed to 40 orders or thinner powder rapidly with common beater grinder; 3. then raw material propolis powder is joined lentamente being equipped with in the propolis extraction pot that extracts solvent of stirring; 4. extracting solvent is 95% (or more than 95%) edible ethanol or dehydrated alcohol; 5. raw material propolis powder and the ratio of extracting solvent are 1 than 3.5-4.5 (W/W); 6. mixing speed is 8-15 rev/min; 7. extracting temperature is normal temperature laboratory; 8. extraction time is 8-12 hour; 9. filter type adopts micropore fine filtration technology to make with extra care filtration; 10. concentrate and reclaim solvent and drying under reduced pressure, pulverize after cooling is placed and promptly get the deleading propolis powder of purifying.Finally make the purity of propolis antivirus antioxidizing active extract reach 100%, solid content reaches more than 99.9%; Content of heavy metal lead≤0.01ppm, the content of propolis total flavones reaches (wherein three kinds of flavone sums of pinocembrin Pinocembrin, chrysin Chrysin and galangin Galangin reach more than 8%, adopt high performance liquid chromatography-HPLC test) more than 10%.
---in the manufacture method of purification deleading propolis powder provided by the invention, disclosed the propolis purification process, comprise: 1. purify Cera Flava and insoluble impurities: selection concentration is that edible ethanol or the dehydrated alcohol more than 95% or 95% made the extraction solvent, and under the condition of normal temperature laboratory, stir extraction, be that the micropore fine filtration technology of 100 nanometers is made with extra care filtration finally with the aperture; 2. it is residual to remove organochlorine pesticide: adopt the adsorbent resin that chlorinated organics is had a selective absorption that organochlorine pesticide is carried out selective absorption and remove; 3. desensitization: purify insoluble impurities, extract at room temperature to remove the hot ethanol soluble substance and to adopt adsorbent to carry out the adsorbing and removing sensitizer; 4. remove such as plumbous and hydrargyrum one class heavy metal: adopt ion exchange resin that heavy metal is had a selective absorption to as plumbous and hydrargyrum one class heavy metal reach and carry out selective absorption as arsenic one class harmful element and remove; 5. remove the nonactive composition of resinae of some solubilities: extract at room temperature is with the nonactive composition of resinae of the solubility of removing the hot ethanol stripping, is that the micropore fine filtration technology of 100 nanometers is made with extra care filtration with the aperture finally.
---the deleading propolis powder that the invention provides the method purification that discloses with the present invention is the health food containing propolis that primary raw material is made, the content range of deleading propolis powder of wherein purifying is 10-100%, the content range of additive is 0-90%, additive is respectively: Lac regis apis dry powder, bee pollen, broker wall bee pollen and extract thereof, queen bee embryo (larva) dry powder and extract thereof, Nidus Vespae and extract thereof, the pupa albumen extract, Pollen Typhae pollen, breaking cellular wall Pollen Typhae pollen and extract thereof, Pollen Pini, breaking cellular wall Pollen Pini and extract thereof, oatmeal and extract thereof, Fructus Cucurbitae moschatae powder and extract thereof, juice powder, the vegetable juice powder, Se-rich xianggu powder and extract thereof, health must selenium powder, selenium-enriched edible mushroom powder and extract thereof, smart powder of Ganoderma herb and ganoderan extract thereof, Ganoderma spore powder, Ganoderma spore powder with cellular wall broken and extract thereof, biological calcium powder, vitamin A, the vitamin B powder, vitamin c powder, vitamin D powder, vitamin E, other vitamin, the compound vitamin powder, ginkgo leaf extract powder and Folium Ginkgo flavone extract thereof, smart powder of Folium Kaki and Folium Kaki flavone extract thereof, be medicine be again the natural animal-plant activity extract of food, panax ginseng extractum powder and extract thereof, Radix Panacis Quinquefolii and red sage root refined powder and extract thereof, natural Chinese medicinal herb vegetable active extract, lactose, dextrin, α, beta, gamma-cyclodextrin and derivant thereof, maltodextrin, starch, superfine SiO 2, magnesium hydroxide, calcium carbonate, mannitol, xylitol, microcrystalline Cellulose, polyvinylpyrrolidone, Polyethyleneglyeol, polyoxyethylene (40) monostearate (S-40), Poloxamer 188, gelatin, stearic acid, sodium stearate etc. or its compositions.
---it is the health food containing propolis that raw material is made that the present invention also provides the purification deleading propolis powder that makes with described method, and described health product are confection, chewing gum, chewing gum, such as dosage forms such as buccal tablet and effervescent tablet one class tablet, capsule, granule, instant and drop pill.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, described health product just like: 1. selenium spirit bee glue powder capsule: deleading propolis powder 20-50% wherein purifies, Ganoderma spore powder with cellular wall broken 20-25%, selenium-enriched edible mushroom powder or health must selenium powder 20-25%, cyclodextrin 0-40%; 2. bee glue powder capsule, the deleading propolis powder 75-100% that wherein purifies, cyclodextrin 0-25% or polyvinylpyrrolidone 0-25% or superfine SiO 2 0-25%; 3. the outstanding capsule of propolis ganoderma lucidum, 50 parts of weight of Ganoderma spore powder with cellular wall broken wherein, 25 parts of weight of purification deleading propolis powder, 25 parts of weight of cyclodextrin, 20 parts of weight of queen bee embryo dry powder; 4. the compound extraordinary bee pollen electuary of propolis, the deleading propolis powder 10% of wherein purifying, bee pollen 50-90%, other additive 0-40%.
---what the invention provides that described method makes is the health product that having of raw material manufacturing improved sleep and chemical liver injury had the health-care effect of auxiliary protection with purification deleading propolis powder; described health product are selenium spirit bee glue powder capsule; deleading propolis powder 20-50% wherein purifies; Ganoderma spore powder with cellular wall broken 20-25%; selenium-enriched edible mushroom powder (health must selenium powder) 20-25%, cyclodextrin 0-40%.
---what the invention provides that described method makes is the auxiliary health product that suppress the health-care effect of tumor of having of raw material manufacturing with purification deleading propolis powder, described health product are honeybee Lingbao City capsule, wherein Ganoderma spore powder with cellular wall broken 50%, purification deleading propolis powder 25%, broker wall bee pollen (Flos Camelliae Japonicae) 0-10%, the smart powder 0-10% of Ganoderma herb, queen bee embryo dry powder 10%, Lac regis apis dry powder 5%.
---what the invention provides that described method makes is the auxiliary health-care effect health product that suppress tumor of having of raw material manufacturing with purification deleading propolis powder, and described health product are strong honeybee curing capsule, the deleading propolis powder 50% of wherein purifying, Ganoderma spore powder 50%.
---what the invention provides that described method makes is having blood lipid regulation and improving gastrointestinal function, facilitating digestion, loosening bowel to relieve constipation, gastric mucosa is had the health-care effect health product of auxiliary protection function of raw material manufacturing with purification deleading propolis powder; described health product are bee glue powder capsule; the deleading propolis powder 75-100% that wherein purifies, cyclodextrin 0-25% or polyvinylpyrrolidone 0-25% or superfine SiO 2 0-25%.
---the invention provides that described method makes with purification deleading propolis powder be the raw material manufacturing have cleaning throat and moistening larynx and radiation-resistant health-care effect health product, described health product are propolis dripping pill, the deleading propolis powder 10-50% that wherein purifies, Polyethylene glycol or polyoxyethylene (40) monostearate 50-90%.
---what the invention provides that described method makes is having prevention and treating the health product of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza of raw material manufacturing with purification deleading propolis powder, described health product are bee glue powder capsule, the deleading propolis powder 75-100% that wherein purifies, cyclodextrin 0-25% or polyvinylpyrrolidone 0-25% or superfine SiO 2 0-25%.
---what the invention provides that described method makes is having prevention and treating the health product of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza of raw material manufacturing with purification deleading propolis powder, described health product are propolis tablet, the deleading propolis powder 50-100% that wherein purifies, other adjuvant 0-50%.
---what the invention provides that described method makes is having prevention and treating the health product of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza of raw material manufacturing with purification deleading propolis powder, described health product are propolis dripping pill, the deleading propolis powder 10-50% that wherein purifies, Polyethylene glycol or polyoxyethylene (40) monostearate 50-90%.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, described health product are full component solubility propolis liquid goods and propolis liquid goods, the content range of deleading propolis powder of wherein purifying is 10-50%, the content range of solvent is 50-90%, and solvent is respectively 95% edible ethanol, ethanol, glycerol, polyglycereol, propylene glycol (Propylene glycol), polypropylene glycol (Polypropylene glycol) and Polyethylene Glycol (Polyethylene glycol) or its compositions.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, described health product are full component solubility propolis liquid and goods thereof, dosage forms such as concentrated solution for example for oral administration, oral cavity aerosol, spray, drop and soft capsule.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, described health product are full component solubility propolis liquid goods, propolis liquid food for example, as: 1. Beijing propolis liquid, the deleading propolis powder 5-50% that wherein purifies, 95% edible ethanol or/and propylene glycol (Propylene glycol) or/and Polyethylene Glycol (Polyethylene glycol) or its compositions 50-95%; 2. propolis vigor soft capsule, the deleading propolis powder 10-50% that wherein purifies, 95% edible ethanol or/and propylene glycol (Propylene glycol) or/and Polyethylene Glycol (Polyethylene glycol) or its compositions 50-90%.
---the invention provides that described method makes with purification deleading propolis powder be the raw material manufacturing have radioprotective and blood sugar regulation health product, described health product are the healthy goods of full component solubility propolis liquid, its purification deleading propolis powder 5-50%, 95% edible ethanol or/and propylene glycol (Propylene glycol) or/and Polyethylene Glycol (Polyethyleneglycol) or its compositions 50-95%.
---what the invention provides that described method makes is having cleaning throat and moistening larynx and beauty treatment, the acne of dispelling, functions of removing chloasma, improving the health product of skin moisture and oil content health-care effect of raw material manufacturing with purification deleading propolis powder, described health product are full component solubility propolis liquid or propolis vigor soft capsule, the deleading propolis powder 10-50% that wherein purifies, Polyethylene Glycol (Polyethylene glycol) 50-90%.
---what the invention provides that described method makes is having prevention and treating the health product of the effect of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza of raw material manufacturing with purification deleading propolis powder, described health product are full component solubility propolis liquid or its propolis vigor soft capsule, the deleading propolis powder 10-50% that wherein purifies, Polyethylene Glycol (Polyethylene glycol) 50-90%.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, described health product are full component solubility external propolis liquid, the content range of deleading propolis powder of wherein purifying is 5-20%, the content range of solvent is 80-95%, solvent be respectively ethanol, propylene glycol (Propylene glycol), polypropylene glycol (Polypropylene glycol), Polyethylene Glycol (Polyethylene glycol) but, the transdermal of externals such as isopropyl alcohol, dimethyl sulfoxide, glycerol and glyceric acid fat promotes lyosoption.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, and described health product are full component solubility propolis liquid and such as aerosol for external use, spray, eye drop, ear drop, baths, liniment and unguentum etc.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, a kind of molecular clathrate of the deleading propolis powder of purifying, microcapsule, microsphere and nanoparticle etc. have targeting and have the instant propolis powder new formulation of slow-releasing and controlled-releasing action, it is characterized in that: the content range of purification deleading propolis powder is 10-50%, enclose, encapsulation, the content range of microsphere and nanoparticle carrier material is 50-90%, enclose, encapsulation, microsphere and nanoparticle carrier material are respectively: 1. carbamide (urea, carbonyl diamine), thiourea, deoxycholic acid and cyclodextrin and derivant thereof etc.; 2. gelatin, polyvinylpyrrolidone, polyacrylic acid, hydroxy methocel and hydroxyethyl-cellulose etc.; 3. ethyl cellulose, gelatin, arabic gum, polylactic acid, methyl acrylate copolymer, methacrylic acid copolymer, polyamide, polyvidone and VPVAC etc.; 4. gelatin, methyl methacrylate, methyl 2-cyanoacrylate, cyanacrylate, Tisuacryl, isobutylcyanoacrylate and methylmethacrylate etc.
---what the invention provides that described method makes is the health product that raw material is made with purification deleading propolis powder, and propolis flavone content reaches more than 90% in the described health product, and wherein pinaster element, chrysin and three kinds of flavone sums of galangin reach more than 72%.Described health product are the new formulations such as molecular clathrate, microcapsule, microsphere and nanoparticle of confection, chewing gum, chewing gum, tablet (comprising buccal tablet and effervescent tablet), oral cavity aerosol, spray, capsule, granule, instant, beverage, soft gelatin capsule and drop pill and propolis flavone.The production of described health product propolis flavone and preparation method adopt: macroporous resin adsorption eluting preparation method, column chromatography and supercritical fluid extraction preparation method etc.
Specifically realize as follows:
1. the molecular clathrate of propolis antivirus antioxidizing active extract and microcapsule formulation
1. the molecular clathrate preparation of propolis antivirus antioxidizing active extract, be meant propolis vigor element with molecular forms by enclose or be embedded in the molecule tubular structure of novel enclose material (as beta-schardinger dextrin-etc.) formed ultra micron to disperse thing be molecular clathrate (Inclusion Compound), claim molecular microcapsule again.Its molecule stereo structure is cylindrical shape in the form of a ring, and outside diameter of cylinder is about the 1.3-1.4 nanometer, and internal diameter is about the 0.7-0.8 nanometer, and the high 0.7-0.8 nanometer that is about of tube is a kind of nanoscale molecular microcapsule.
2. preparation technology: fully stir and oven dry pulverizing-propolis vigor element clathrate solid material powder-dissolving and filtration-propolis vigor element inclusion complex in solution-spray drying-instant powder of propolis clathrate after the plain pure powder of propolis vigor-with the dissolution with solvents of 2 times of amounts and filtrations-lysate adds excipient.
3. the characteristics of molecular clathrate new formulation: the molecular clathrate of propolis antivirus antioxidizing active extract can make propolis vigor element change to the permeability of film with combination of proteins, thereby increased the absorption of propolis vigor element, improved the bioavailability of propolis vigor element, reduce the using dosage of propolis vigor element, improved the mouthfeel of propolis vigor element.
2. the microsphere of propolis antivirus antioxidizing active extract and nanoparticle preparation
Nanoparticle (Nanocapsules) is a nanocapsule, is a kind of directed delivery system that has, its similar microcapsule, and dispersion is than microcapsule micronize more, the molecular association complex of likeness in form colloidal ion.The nanoparticle preparation of propolis antivirus antioxidizing active extract is exactly the nanoparticle of the encapsulation propolis vigor element made with natural high molecular substance such as gelatin, albumin and cellulose family etc., and diameter is about the 50-500 nanometer.
The invention solves the technical barrier in five these fields of taking off such as removing heavy metal lead and desensitization, and make its technical specification reach the top standard.For example, wherein: 1. heavy metal lead≤0.01ppm, hydrargyrum≤0.01ppm, arsenic≤0.01ppm; 2. removed main sensitization source, through Cavia porcellus anaphylaxis contrast experiment, its result is: positive before the desensitization, the desensitization back is negative; 3. Cera Flava and insoluble impurities are 0 to be that propolis active substance content is 100%; 4. organochlorine pesticide is residual does not detect; 5. the nonactive composition of soluble resin class is 0.Provide standardized purification deleading propolis powder raw materials for production for producing and make new formulations such as serial propolis food, the healthy goods of propolis and propolis medicine.
Compared with prior art, the present invention has following distinguishing feature and good effect:
1) the propolis antivirus antioxidizing active extract deleading propolis powder purity height of promptly purifying reaches 100%.
2) can not add any antiadhesives and the excipient bonding of can not luming under 40 ℃ of hot conditionss, and present the mobile fabulous powder body of foresythia, its solubility solid content is up to 99.9%;
3) demarcated antivirus antioxidizing active material---propolis total flavones and caffeinic content in the standard China propolis extract with high performance liquid chromatography first.Total flavone content in propolis has reached 10%, wherein pinocembrin (Pinocembrin), galangin (Galangin) and chrysin (Chrysin) sum 〉=8%; And the content of caffeic acid (Caffeic acid) is 1-1.5%;
4) bee glue purifying of Sheng Chaning has: purified Cera Flava and insoluble impurities, removed organochlorine pesticide residual, desensitize, removed heavy metal (plumbous and hydrargyrum arsenic etc.) and pollute and removed the nonactive composition of some soluble resin classes etc. five and take off new feature, wherein propolis removing heavy-metal (lead and hydrargyrum arsenic etc.) technology and desensitization technology are in the leading level in the world;
5) a series of production technologies and manufacturing process thereof involved in the present invention not only are adapted to Chinese propolis, and are adapted to the raw material propolis that the propolis production countries and regions in the worldwide such as Brazilian propolis, Japanese propolis, Korea S's propolis, Canadian propolis, New Zealand's propolis, U.S.'s propolis, Russian propolis, India's propolis, Korea's propolis and Taiwan propolis are produced;
6) solved full component water-soluble bee glue liquid production technology;
7) on the basis of full component water-soluble bee glue liquid production technology, innovated propolis soft capsule production technology, and propolis antivirus antioxidizing active thing is existed with molecularity, content can reach 50%.
8) new formulation production technologies such as the instant propolis powder of molecular clathrate, microcapsule, microsphere and nanoparticle have been innovated.
9) promptly purify deleading propolis powder and preparation thereof of the propolis antivirus antioxidizing active extract that utilizes the technology of the present invention to produce, its advantage has greatly improved the antibacterial preservation antioxidation activity and the anti-virus ability of bee glue product, make nearly thousand kinds of effective active components in the propolis keep getting off intactly, the fresh and alive food that makes the higher fatty acid high protein that is applicable in the preservation antioxidation is (as fruit, fresh meat, fish, shrimp) and beverage (fresh milk, fruit juice, fruit juice, kernel protein beverage etc.), extend to more than 2 years even the longer time by storing 2-8 hour originally with regard to going bad.
Below by instantiation the present invention is done further narration, but this is not to be concrete qualification to the scope of the invention.
Specific embodiments
Promptly the purify manufacturing example of deleading propolis powder of embodiment one, propolis antivirus antioxidizing active extract
1. with the raw material propolis of 200 kilograms of weight pre-freeze 24 hours under the condition below 0 ℃;
2. the raw material propolis after the pre-freeze is crushed to 40 purpose powder rapidly with common beater grinder;
3. 200 kg feed material propolis powder are joined lentamente in the propolis extraction pot that 800 kilogram of 95% edible ethanol is housed that is stirring then;
4. stir and extract after 12 hours, quiet to precipitating 12 hours, wherein mixing speed is 8 rev/mins, and the extraction temperature is a normal temperature laboratory;
5. adopting the aperture is that the micropore fine filtration technology of 100 nanometers is made with extra care filtration to supernatant;
6. remove heavy metal (lead and hydrargyrum arsenic etc.): adopt ion exchange resin that heavy metal (lead and hydrargyrum arsenic etc.) is carried out selectivity ion exchange and remove;
7. reclaim under reduced pressure ethanol and concentrate drying cool off placement back crushing screening and promptly get 80 kilograms of purification deleading propolis powder.
After measured: the propolis antivirus antioxidizing active extract promptly purify the deleading propolis powder 1. purity be 100%; 2. solid content is 99.9%; 3. heavy metal lead≤0.0lppm; 4. the content of propolis total flavones 〉=10%, three kinds of important antivirus antioxidizing effective active components wherein: pinocembrin (5,7-dihydroxy flavanone Pinocembrin), chrysin (5,7-dihydroxyflavone Chrysin) and galangin (3,5,7-trihydroxyflavone alcohol Galangin) sum 〉=8%, other propolis flavone such as rutin (Rutin), myricetin (Myricetin), Quercetin (Quercetin), the luxuriant and rich with fragrance alcohol of camphane (Kaempferol), apigenin (Apigenin), morin (Motin) and isorhamnetin (Isorhamnetin) equal size sum are 2%, the content of caffeic acid (Caffeicacid) is 1.1%, and propolis flavone and caffeinic detection method all adopt high performance liquid chromatography (HPLC).
The technical matters embodiment is taken off in embodiment two, propolis antivirus antioxidizing active extract purification five
1. purify Cera Flava and insoluble impurities:
1. with the raw material propolis of double centner weight pre-freeze 24 hours under the condition below 0 ℃;
2. the raw material propolis after the pre-freeze is crushed to 40 purpose powder rapidly with common beater grinder;
3. double centner raw material propolis powder is joined lentamente in the propolis extraction pot that 400 kilogram of 95% edible ethanol is housed that is stirring then;
4. stir and extract after 12 hours, quiet to precipitating 12 hours, wherein mixing speed is 10 rev/mins, and the extraction temperature is a normal temperature laboratory;
5. quiet to after the propolis supernatant be that the micropore fine filtration technology of 100 nanometers is made with extra care filtration finally with the aperture.
2. it is residual to remove organochlorine pesticide:
6. adopt adsorbent resin that chlorinated organics is had a selective absorption to carry out selective absorption and remove to organochlorine pesticide is residual;
3. remove the nonactive composition of resinae of some solubilities:
7. extract at room temperature is with the nonactive composition of resinae of the solubility of removing the hot ethanol stripping, is that the micropore fine filtration technology of 100 nanometers is made with extra care filtration with the aperture finally;
4. desensitization:
8. on the basis of the nonactive composition of resinae that purifies insoluble impurities and some solubilities, adopt adsorbent such as activated earth that sensitizer is carried out adsorbing and removing again, be that the micropore fine filtration technology of 100 nanometers is made with extra care filtration finally with the aperture.
5. remove heavy metal (lead and hydrargyrum arsenic etc.):
9. adopting ion exchange resin that heavy metal (plumbous and hydrargyrum arsenic etc.) is carried out selectivity ion exchange and remove, is that the micropore fine filtration technology of 100 nanometers is made with extra care filtration with the aperture finally.
6. reclaim under reduced pressure ethanol and concentrate drying:
10. reclaim under reduced pressure ethanol and concentrate drying cool off placement back crushing screening and promptly get 40 kilograms of propolis vigor elements.After measured, the propolis antivirus antioxidizing active extract is that the technical specification of propolis vigor element is:
1. Cera Flava and insoluble impurities are 0 to be that propolis active substance content is 100%;
2. organochlorine pesticide is residual does not detect;
3. the nonactive composition of soluble resin class is 0;
4. through Cavia porcellus anaphylaxis contrast experiment, its result is: positive before the desensitization, the desensitization back is negative;
5. heavy metal lead≤0.01ppm, hydrargyrum≤0.01ppm, arsenic≤0.01ppm.
Embodiment three, be the propolis food embodiment that main material production is made with purification deleading propolis powder
1. selenium spirit propolis powder (capsule): purification deleading propolis powder 20%, Ganoderma spore powder with cellular wall broken 20%, selenium-enriched edible mushroom powder 20%, cyclodextrin 40%.
2. propolis powder (capsule): purification deleading propolis powder 85%, cyclodextrin 15%.
3. propolis ganoderma lucidum outstanding person (capsule): 50 parts of weight of Ganoderma spore powder with cellular wall broken, 25 parts of weight of purification deleading propolis powder, 25 parts of weight of cyclodextrin, 20 parts of weight of queen bee embryo dry powder.
4. the compound extraordinary bee pollen of propolis (electuary): purification deleading propolis powder 10%, bee pollen 90%.
Example four, be the healthy goods example I of propolis that main material production is made with purification deleading propolis powder
1. has the healthy goods of propolis that improve sleep and chemical liver injury is had auxiliary protection function---selenium spirit propolis powder (capsule).It consists of: purification deleading propolis powder 20-50%, Ganoderma spore powder with cellular wall broken 20-25%, selenium-enriched edible mushroom powder (health must selenium powder) 20-25%, cyclodextrin 0-40%.
2. has healthy goods---the propolis powder (capsule) of the propolis that improves gastrointestinal function (facilitating digestion/loosening bowel to relieve constipation/gastric mucosa is had auxiliary protection function).It consists of: purification deleading propolis powder 75-100%, superfine SiO 2 0-25%.
3. the propolis healthy goods---honeybee Lingbao City (capsule) that has auxiliary function of tumor inhibition.It consists of: Ganoderma spore powder with cellular wall broken 50%, purification deleading propolis powder 25%, broker wall bee pollen (Flos Camelliae Japonicae) 0-10%, the smart powder 0-10% of Ganoderma herb, queen bee embryo dry powder 10%, Lac regis apis dry powder 5%.
4. the healthy goods of propolis---the strong honeybee spirit (capsule) that has auxiliary function of tumor inhibition.It consists of: purification deleading propolis powder 50%, Ganoderma spore powder 50%.
Example five, be the healthy goods example II of propolis that main material production is made with purification deleading propolis powder
1. has healthy goods---the propolis powder (capsule) of the propolis that prevents and treat each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza effect.It consists of: purification deleading propolis powder 75-100%, cyclodextrin 0-25%.
2. has healthy goods---the propolis tablet (tablet) of the propolis that prevents and treat each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza effect.It consists of: purification deleading propolis powder 50-100%, other adjuvant 0-50%.
3. has healthy goods---the propolis dripping pill of the propolis that prevents and treat each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza effect.It consists of: purification deleading propolis powder 10-50%, Polyethylene glycol or polyoxyethylene (40) monostearate (S-40) 50-90%.
Example six, be the propolis food embodiment that main material production is made with full component solubility propolis liquid
1. in the propolis liquid of Beijing: purification deleading propolis powder 10-50%, Propylene glycol is or/and Polyethyleneglycol or its compositions 50-90%.
2. in the propolis vigor soft capsule: purification deleading propolis powder 10-50%, Polyethylene glycol 50-90%.
Example seven, be the propolis health food embodiment that raw material production is made with full component solubility propolis liquid
1. have in Beijing propolis liquid of health cares such as immunomodulating and blood sugar regulation: purification deleading propolis powder 10-50%, Propylene glycol is or/and Polyethylene glycol or its compositions 50-90%.
2. have in the propolis vigor soft capsule of health cares such as blood sugar regulation and beauty treatment (acne/functions of removing chloasma of dispelling/improve skin moisture and oil content): purification deleading propolis powder 10-50%, Polyethylene glycol50-90%.
3. have in the propolis dripping pill of health cares such as cleaning throat and moistening larynx and radioprotective: purification deleading propolis powder 10-50%, Polyethylene glycol 50-90%.
Example eight, be the bee glue product example I that raw material production is made with full component solubility propolis liquid
With full component solubility propolis liquid is bee glue product-rheumatism essence that main material production is made, and its manufacture method is as follows:
1. the fresh Venenum apis of 0.5-1% is added in the full component solubility of the 5-20% propolis liquid, and then add an amount of Mentholum, essential oil and eugenol etc., fully mix the back packing and promptly get the rheumatism essence.
2. the rheumatism essence is a kind of externally applied liniment, is mainly used in rheumatism, rheumatoid arthrosis osteodynia, mosquito bite.
Example nine, be the healthy goods example II of propolis that raw material production is made with full component solubility propolis liquid
1. have the prevention and treat in the propolis vigor soft capsule of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza effect: purification deleading propolis powder 10-50%, Polyethylene glycol 50-90%.
2. have the prevention and treat in the propolis dripping pill of each phase gastritis, gastric ulcer and duodenal ulcer, diabetes, cardiovascular and cerebrovascular disease, heart disease, hyperlipidemia, hypertension, coronary heart disease, psoriasis and viral hepatitis and influenza effect: purification deleading propolis powder 10-50%, Polyethylene glycol 50-90%.
Example ten, be primary raw material with purification deleading propolis powder, with supercritical liquid extraction technique production and preparation propolis flavone embodiment
1. supercritical extraction technique is the efficient abstraction technique that development in recent years is got up, and it is to utilize the property of medium under supercriticality, under different pressure and temperatures, can optionally extract the active substance composition in the raw material.
2. the present invention adopts the supercritical fluid extraction machine, with CO
2Make medium the propolis flavone in the purification deleading propolis has been carried out optionally extraction.
3. propolis flavone supercritical extraction liquid is through concentrate under reduced pressure at low temperature, and promptly gets propolis flavone after the drying and crushing.After measured: propolis flavone content 95%, wherein pinaster element, chrysin and galangin sum are 76%, detection method adopts high performance liquid chromatography (HPLC).
Example 11, be main material production and make propolis flavone goods example I with the supercritical propolis flavone
With the supercritical propolis flavone is main material production propolis flavone drop pill, and its manufacture method is as follows:
1. after using Polyethylene glycol with the fusion of supercritical propolis flavone, directly drip system propolis flavone drop pill.
2. in the propolis flavone drop pill: supercritical propolis flavone 50%, Polyethylene glycol 50%.
After measured: in the propolis flavone drop pill---propolis flavone content 47.5%, wherein pinaster element, chrysin and galangin sum are 38%, detection method adopts high performance liquid chromatography (HPLC).
Example 12, be main material production and make propolis flavone goods example II with the supercritical propolis flavone
With the supercritical propolis flavone is the instant powder of main material production propolis flavone, and its manufacture method is as follows:
1. with the supercritical propolis flavone with organic solvent dissolution after, directly add in the saturated solution of cyclodextrin, and constantly stir, the cooling post crystallization promptly gets the instant powder of propolis flavone.
2. in the instant powder of propolis flavone: supercritical propolis flavone 10%, cyclodextrin 90%.
After measured: in the instant powder of propolis flavone---propolis flavone content 9.5%, wherein pinaster element, chrysin and galangin sum are 7.6%, detection method adopts high performance liquid chromatography (HPLC).
Claims (1)
1. method for preparing the deleading propolis is characterized in that this method comprises:
1, with the raw material propolis more than-4 ℃ to 0 ℃ the temperature pre-freeze,
2, after the pre-freeze, be crushed to below 40 orders,
3, room temperature and under 8-15 rev/min of mixing speed, with ethanol extraction 8-12 hour that equals greater than 95% percetage by weight, propolis powder was 1 than 3.5-4.5 with equaling weight ratio greater than the ethanol extraction solvent of 95% percetage by weight,
4, filter with micropore fine filtration technology,
5, after the secondary filter, use the resin that chlorinated organics is had a selective absorption to remove,
6, carry out the adsorbing and removing sensitizer with the activated earth adsorbent,
7, use the ion exchange resin that heavy metal is had a selective absorption to carry out selective absorption, remove heavy metal,
8, extract at room temperature is with the resinae non-active ingredient of the solubility of removing the hot ethanol stripping,
9, be that the micropore fine filtration technology of 100 nanometers is made with extra care filtration with the aperture,
10, the propolis powder that drying under reduced pressure is pulverized.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011417633A CN1220497C (en) | 2001-09-18 | 2001-09-18 | Antivirus antioxidizing active extract of proplis and its molecular inclusion compound |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011417633A CN1220497C (en) | 2001-09-18 | 2001-09-18 | Antivirus antioxidizing active extract of proplis and its molecular inclusion compound |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1342464A CN1342464A (en) | 2002-04-03 |
CN1220497C true CN1220497C (en) | 2005-09-28 |
Family
ID=4676392
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB011417633A Expired - Fee Related CN1220497C (en) | 2001-09-18 | 2001-09-18 | Antivirus antioxidizing active extract of proplis and its molecular inclusion compound |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1220497C (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9719970B2 (en) | 2012-11-30 | 2017-08-01 | Waters Technologies Corporation | Methods and apparatus for the analysis of vitamin D metabolites |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1313020C (en) * | 2003-12-25 | 2007-05-02 | 南昌大学 | Combination use of absorption and ion exchange for removing lead from propolis |
CN1303984C (en) * | 2005-07-18 | 2007-03-14 | 北京联合大学生物化学工程学院 | Method for prparing bee glue-ethyl cellulose microcapsule |
CN101518552B (en) * | 2009-04-10 | 2012-01-04 | 南京师范大学 | Pine pollen and cyclodextrin composition, method for preparing same, use of same in preparation of health-care products for resisting fatigue and improving immunity of organisms |
CN102078337A (en) * | 2011-01-14 | 2011-06-01 | 哈药集团密山蜂业有限公司 | Propolis natural reduction extraction process method |
CN103719982B (en) * | 2013-12-26 | 2015-11-04 | 福建农林大学 | Fruit drink of a kind of preservative free and preparation method thereof |
CN103652532B (en) * | 2013-12-26 | 2015-03-18 | 陈静 | Healthy food prepared from qinghai-tibet plateau bee product and Chinese herbal medicine |
CN104127449A (en) * | 2014-08-12 | 2014-11-05 | 安徽衡济堂灵芝产业开发有限公司 | Combined ganoderma lucidum capsule for auxiliary inhibition on tumor and preparation method thereof |
CN104997716B (en) * | 2015-08-17 | 2020-03-17 | 杭州华胄科技有限公司 | Oral care product containing propolis alcohol extract and having anti-helicobacter pylori effect |
CN105169373A (en) * | 2015-09-10 | 2015-12-23 | 夏顺刚 | Nutrition buccal tablet suitable for children to take and preparation method of nutrition buccal tablet |
CN105963237B (en) * | 2016-07-06 | 2019-02-22 | 广东科玮生物技术股份有限公司 | A kind of Novel mouthwash and its preparation method and application |
CN106581078A (en) * | 2017-03-01 | 2017-04-26 | 四川天福精细化工有限公司 | Oral desensitization paste and preparation method thereof |
CN108593826B (en) * | 2018-06-04 | 2020-05-19 | 中国农业科学院蜜蜂研究所 | Method for identifying source of bee pollen |
CN109198084A (en) * | 2018-09-14 | 2019-01-15 | 杭州和蜂园保健品有限公司 | A kind of preparation method of propolis tea bag |
CN111956641B (en) * | 2019-12-30 | 2021-11-02 | 云南汉盟制药有限公司 | Flavonoid compound composition and preparation method thereof |
CN113750120B (en) * | 2021-10-11 | 2023-10-24 | 北京德蜂堂健康科技有限公司 | Propolis extract and production process and application thereof |
-
2001
- 2001-09-18 CN CNB011417633A patent/CN1220497C/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9719970B2 (en) | 2012-11-30 | 2017-08-01 | Waters Technologies Corporation | Methods and apparatus for the analysis of vitamin D metabolites |
Also Published As
Publication number | Publication date |
---|---|
CN1342464A (en) | 2002-04-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1220497C (en) | Antivirus antioxidizing active extract of proplis and its molecular inclusion compound | |
CN1289079C (en) | Use of an opuntia ficus-indica extract and compounds isolated therefrom for protecting nerve cells | |
RU2355187C1 (en) | Feed supplement for farm livestock | |
CN1840166A (en) | Modern Chinese medicinal oral liquid of 'Wen Dan Tang' and preparation method thereof | |
Khursheed et al. | Phytochemical and pharmacological investigations on adhatoda zeylanica (medic.): A review | |
CN103566150B (en) | A kind of compositions and application thereof with the integration of edible and medicinal herbs food of effect for reducing blood fat | |
CN101991606B (en) | Preparation method of solid water-soluble lead-removed propolis | |
Acharya et al. | Medicinal uses of amla, Phyllanthus emblica L.(Gaertn.): a prospective review | |
CN1202834C (en) | Application of the effective part of Chinese medicinal material lindley eupatorium herb in preparing antiviral medicine | |
CN1242696C (en) | Health-care rose food | |
CN1370566A (en) | Compounding process of dermatosis treating medicine | |
CN1215595A (en) | External use medicine for treating soft tissue injury and its producing method | |
RU2303993C1 (en) | Treatment and prophylaxis means applicable for treating young agricultural animals and method for preventing and treating calves for gastrointestinal tract diseases | |
CN1939480A (en) | Chinese-medicinal compound preparation for treating injury | |
CN1634371A (en) | Pterodontic preparation for treating infectious diseases and preparation method thereof | |
CN1557824A (en) | Silkworm excrement total alkaloid and its preparation method | |
Chattopadhyay et al. | Preliminary phytochemical screening and in vitro evaluation of anthelmintic activity of leaves and stem bark of Mangifera Indica L.(Anacardiaceae) | |
CN105456530B (en) | Preparation method of quick-release and slow-release traumatic injury bone-growing tablet | |
CN1679703A (en) | Medicinal preparation containing notoginseng and ginkgo leaf for cardio-cerebral blood vessel diseases and its preparing method | |
CN1054740C (en) | Perfume containing the abstract of viscera and tissue of snake and frog | |
Naman et al. | Anti-Anaemic Potential of Methanolic Leaf Extract of Mucuna Pruriens on Phenylhydrazine (Phz) Induced Anaemic Albino Wistar Rats | |
CN1330296C (en) | Propolis soft capsule and its preparation method | |
CN108210877A (en) | Treat the preparation method of cardiopathic pharmaceutical composition | |
CN1768775A (en) | Prescription containing sweet clover component and its formulation | |
CN107223960A (en) | It is a kind of that there is composition of improvement prostate gland symptoms and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C57 | Notification of unclear or unknown address | ||
DD01 | Delivery of document by public notice |
Addressee: Jiang Deyong Document name: Notice of first review |
|
C57 | Notification of unclear or unknown address | ||
DD01 | Delivery of document by public notice |
Addressee: Jiang Deyong Document name: Notice of first review |
|
C57 | Notification of unclear or unknown address | ||
DD01 | Delivery of document by public notice |
Addressee: Jiang Deyong Document name: Deemed as a notice of withdrawal (Trial) |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
DD01 | Delivery of document by public notice |
Addressee: Jiang Deyong Document name: Review of business letter |
|
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050928 Termination date: 20190918 |