CN121358762A - 经修饰的重组腺相关病毒（aav）结合性蛋白质及aav的纯化方法 - Google Patents

经修饰的重组腺相关病毒（aav）结合性蛋白质及aav的纯化方法

Info

Publication number
CN121358762A
CN121358762A CN202480033591.4A CN202480033591A CN121358762A CN 121358762 A CN121358762 A CN 121358762A CN 202480033591 A CN202480033591 A CN 202480033591A CN 121358762 A CN121358762 A CN 121358762A
Authority
CN
China
Prior art keywords
seq
substitution
aav
alanine
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202480033591.4A
Other languages
English (en)
Chinese (zh)
Inventor
岩渊圭笃
栗原健人
真锅友理子
吉田浩平
大村慧太
田中亨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Publication of CN121358762A publication Critical patent/CN121358762A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202480033591.4A 2023-05-23 2024-05-22 经修饰的重组腺相关病毒（aav）结合性蛋白质及aav的纯化方法 Pending CN121358762A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
JP2023084423 2023-05-23
JP2023-084423 2023-05-23
JP2024-007358 2024-01-22
JP2024007357 2024-01-22
JP2024-007357 2024-01-22
JP2024007358 2024-01-22
JP2024063328 2024-04-10
JP2024-063328 2024-04-10
PCT/JP2024/018896 WO2024242150A1 (ja) 2023-05-23 2024-05-22 改変された組換えアデノ随伴ウイルス（ａａｖ）結合性タンパク質およびａａｖの精製方法

Publications (1)

Publication Number Publication Date
CN121358762A true CN121358762A (zh) 2026-01-16

Family

ID=93589449

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202480033591.4A Pending CN121358762A (zh) 2023-05-23 2024-05-22 经修饰的重组腺相关病毒（aav）结合性蛋白质及aav的纯化方法

Country Status (4)

Country Link
EP (1) EP4717706A1 (https=)
JP (1) JPWO2024242150A1 (https=)
CN (1) CN121358762A (https=)
WO (1) WO2024242150A1 (https=)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011097898A (ja) 2009-11-09 2011-05-19 Sagami Chemical Research Institute 組換えＦｃ受容体およびそれの製造方法
US12070702B2 (en) * 2018-06-14 2024-08-27 Regenxbio Inc. Anion exchange chromatography for recombinant AAV production
CN114746552B (zh) * 2019-11-25 2024-11-08 东曹株式会社 改良腺相关病毒结合性蛋白、该蛋白的制造方法以及使用了该蛋白的腺相关病毒吸附剂
EP4299751A4 (en) * 2021-03-09 2025-08-13 Japan Chem Res METHOD FOR PRODUCING A RECOMBINANT AAV9 VIRION
US20240360424A1 (en) * 2021-08-17 2024-10-31 Ultragenyx Pharmaceutical Inc. Anion-exchange chromatography methods for purification of recombinant adeno-associated viruses
JP7597247B2 (ja) * 2022-01-21 2024-12-10 東ソー株式会社 改変された組換えアデノ随伴ウイルス（ａａｖ）結合性タンパク質及び感染能に基づくａａｖの分析方法

Also Published As

Publication number Publication date
EP4717706A1 (en) 2026-04-01
JPWO2024242150A1 (https=) 2024-11-28
WO2024242150A1 (ja) 2024-11-28

Similar Documents

Publication Publication Date Title
JP7619278B2 (ja) 改良アデノ随伴ウイルス結合性タンパク質、当該タンパク質の製造方法および当該タンパク質を用いたアデノ随伴ウイルス吸着剤
JP7306270B2 (ja) 免疫グロブリン結合性タンパク質
US9051355B2 (en) Filler for affinity chromatography and method for isolating immunoglobulin
WO2014146350A1 (zh) 一类突变的具有高耐碱特性的蛋白a及其应用
EP3617315A1 (en) Stable fc binding protein, method for producing said protein, and antibody adsorbent in which said protein is used
JP2025023090A (ja) 改変された組換えアデノ随伴ウイルス（ａａｖ）結合性タンパク質及び感染能に基づくａａｖの分析方法
JP2021045116A (ja) 免疫グロブリン結合性タンパク質
CN121358762A (zh) 经修饰的重组腺相关病毒（aav）结合性蛋白质及aav的纯化方法
JP6848241B2 (ja) Ｆｃ結合性タンパク質を用いたＩｇＧ１の精製方法
JP7683230B2 (ja) アデノ随伴ウイルスの精製方法
JP7782230B2 (ja) アデノ随伴ウイルスの分析方法
CN118591630A (zh) 经改变的重组腺相关病毒(aav)结合性蛋白和基于感染能力的aav分析方法
JP7782229B2 (ja) 血清型に基づくアデノ随伴ウイルスの分析方法
JP2024101078A (ja) アデノ随伴ウイルス吸着剤の洗浄方法
JP7587200B2 (ja) 免疫グロブリン結合性ポリペプチド
JP2022034946A (ja) 免疫グロブリン結合活性を有するポリペプチド
JP2024169303A (ja) 耐アルカリ性の向上したアデノ随伴ウイルス結合性タンパク質
JP2024170270A (ja) アデノ随伴ウイルスのスクリーニング方法
JP2026047184A (ja) レンチウイルスベクターの精製方法
JP2023098806A (ja) アデノ随伴ウイルスの精製方法
JP2022168352A (ja) 免疫グロブリン結合性タンパク質
JP2023079984A (ja) キメラアデノ随伴ウイルスの分析方法
CN121002046A (zh) 免疫球蛋白结合性蛋白质
JP2025183677A (ja) 多重特異性抗体の分離方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination