CN1212068C - Immune formula milk powder and preparation process thereof - Google Patents
Immune formula milk powder and preparation process thereof Download PDFInfo
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Abstract
The present invention provides milk powder with an immunity formulation, which contains the following raw materials by weight parts: 7000000 to 8000000 parts of milk, 4000 to 10000 parts of immunoglobulin, 269 to 720 parts of nucleotide and 2 to 3 parts of beta-carotene. The present invention also provides a preparation method of the milk powder with an immunity formulation, which comprises the following steps: preheating milk to the temperature of 60 to 65 DEG C, homogenizing the milk in 180 bar, heating the milk for sterilization, concentrating the milk in the vacuum, adding immunoglobulin, nucleotide and beta-carotene to the milk, drying the milk in a spray mode so as to obtain the milk powder. The milk powder with an immunity formulation of the present invention has the function of improving immunocompetence.
Description
Technical field
The present invention relates to a kind of milk powder, particularly a kind of immunity formula milk powder and preparation method thereof.
Background technology
Present infant formula both domestic and external, has only anthropomorphic dummy's breast on macroscopical composition, as the ratio of adding whey powder adjustment casein and lactalbumin, increase vegetable oil, strengthen mineral matter and thermal sensitivity vitamin, because do not contain multiple bioactivator in the human milk such as immunoglobulin (Ig), lactoferrin, lactoperoxidase, lysozyme, stimulating growth factor etc. in these products substantially, so infant's autoimmunity ability of edible baby milk powder, easily suffer from diseases such as respiratory tract infection, dysentery, pneumonia, normal genesis and development is slow, problems such as malnutrition.Research human milk bioactivator adds it to realize it in the baby milk powder real breast milkization, has become the research focus of baby milk powder in recent years.
The functional characteristic of the nutriment that relates among the present invention is as follows:
Immunoglobulin (Ig) (Immunoglobulin, be called for short Ig): be that a class has antibody activity or the chemical constitution globulin similar to antibody, have important immunity and physiological regulatory action in animal body, it is one of the most key component of animal vivo immuning system, it can act synergistically with other immune factor, can prevent and improve disease more efficiently, strengthen resistance against diseases, promote normal cell growth, tissue repair and wound recovery from illness.Immunoglobulin (Ig) can strengthen leucocyte and macrophage phagocytic effect and antigen presentation in the passive immunity system, surround, attack the pathogenic bacteria of invading human body, plays an important role in killing the intrusion of pathogenic microbes cell and pathogenic bacteria.Edible immunoglobulin (Ig) can be removed enteric pathogenic bacteria and toxin thereof, promotes the field planting of beneficial bacterium, promotes to digest and assimilate, and adjusts intestinal microenvironment, stimulates organism immune response.
Because the special immune physiological function of immunoglobulin (Ig) becomes focus to its research that is used as functional food additives in the world.Just from colostrum, serum, yolk, extract immunoglobulin (Ig) since the end of the seventies abroad, and developed the immunocompetence additive that contains immunoglobulin (Ig), but reason causes immunoglobulin (Ig) to cost an arm and a leg owing to mass production techniques is immature etc.
Nucleotides research: nucleotides is important low molecular compound in the organism, has many physiological functions, removes the precursor as DNA, RNA, and also the adjusting material as physiology, biochemical process participates in the substance in vivo metabolism.Animal body can be by endogenous synthetic various nucleotides, and do not show typical deficiency disease when lacking.But many studies show that in recent years, when the body ramp or be subjected to when challenge immunity, the nucleotides that some organs, tissue synthesize as intestines, lymph, bone marrow cell can not satisfy the needs of people and animal tissue and cellular metabolism, needs supplemented with exogenous nucleotides to guarantee its tissue growth and normal function.Therefore, the trophism of nucleotides causes concern.Nucleotides existence form, content and absorption in the breast milk: the level of DNA is the 1-12mg/ liter in the breast milk, ribonucleic acid 10-60mg/ liter, and lactation initial stage nucleosides and nucleotide content are the highest, reduce along with time of nursing prolongs gradually.The content of DNA and RNA is lower in the cow's milk, is respectively 1-4mg/ and rises and the 5-19mg/ liter, and how to be present in the whey with nucleotide precursor material orotic acid.
Studies show that the nucleic acid major part of taking in the food resolves into nucleotides, nucleosides, purine, pyrimidine at pipe intestinal digesting, is absorbed at the small intestine epimere.Nucleotides has 5%-10% can be integrated in small intestine, liver, kidney, skeletal muscle, the spleen tissue approximately in the daily ration.When nucleotides in the food is supplied with abundance, organize endogenous synthetic reduction.When having experimental observation to arrive the BF baby in 1994, have 1/3 nucleotides to absorb and enter in the body, and use the milk replacer of low nucleic acid, the nucleic acid of tissue 95% needs from the beginning synthetic.Animal has certain adaptive ability to the metabolism of nucleotides, and under the condition of quick growth and immunological stress such as hunger, infection, the ability enhancing of nucleotides is stayed in the tissue storage.20% of breast milk nucleic acid total amount comes from food nucleotides and relevant material.
The physiological function of nucleotides: nucleotides can be kept immune normal function, as improving the resistance of humans and animals to bacterium, fungal infection, increases antibody and produces, and strengthens cell immunocompetent, stimulates lymphocytosis effect etc.
Nucleotide pair is kept the intestines and stomach normal function effect.At first, extraneous nucleotide can quicken differentiation, the growth of enterocyte and repair, and promotes the maturation of small intestine, and this can cultivate from vitro tissue, the parenteral nutrition test is confirmed.Recovery behind the intestinal tract injury that nucleotides also can quicken to cause because of hunger, radiation, inflammation, ulcer and wound.In addition, daily ration nucleotides can stimulation of bifidobacteria growth, the consumption by infants breast milk and added the milk replacer of nucleotides after, the Bifidobacterium in the ight soil is higher than the amount of enterobacteria, and only two kinds of ratios in the infant faeces of edible milk replacer are just in time opposite.Bifidobacterium can be transformed into lactic acid with sugar, can reduce the pH value in the enteron aisle, suppresses the growth of other harmful bacterium, thereby reduces the generation of infantile diarrhea.
The action effect of nucleotides may be relevant with organism physiology situation, age, simultaneously, between the nucleotides kind that is also given, nucleotides ratio and with the influence of other nutrients, therefore, the trophism of nucleotides also needs further research.
The protein that nucleotides participates in the adjusting liver synthesizes, and is relevant with the normal function of keeping liver.Behind the injured and partially hepatectomized, intestines give nucleotides/nucleosides mixture outward can improve the function of liver injury when the mouse liver, promotes the early recovery of nitrogen balance.
Nucleotides also has antioxidation.The nitrogen oxygen atom of nucleotide base can be caught the free radical that forms in the oxidizing process, reduces the cell membrane that caused by lipid peroxidation and the damage of various DNA.Nucleic acid and related substances all can be used as antioxidant, have the effect of VC sample.Eat the milk replacer that the people suckles or added nucleic acid, the preceding baby's all around in birth back plasma lipoprotein, triglyceride levels are all than not adding nucleic acid group height.Baby food adds nucleotides makes preemie's average weight gain, height, head circumference be higher than control group.
Nucleotides needs: many researchers think that nucleotides is a kind of " half is essential " or " conditionity " nutrient, but the requirement of nucleotides still imperfectly understands.Can make it relatively approach breast-fed babies with the milk replacer feeding infant that replenishes nucleic acid, because functions such as the growth of nucleotide pair intestines and stomach, immunity have important function, some countries have begun to strengthen nucleotides in infant's milk replacer.The European Community had stipulated the upper limit of nucleotides in the infant formula in 1991.Mammal nucleotides needs to reach 0.25%, can only reach about 0.15% at general daily ration content, and young animal replenishes nucleotides can reduce higher endogenous synthesizing of power consumption in the body, is of value to its healthy and growth.Many tests confirm that nucleotides adds effectively under some physiological situation, but what replenish to reach optimised quantity, the kind and ratio and the suitable additional object that add, still not fully aware of, ability such as all ages and classes stage, physiological status lower body self synthesizing ribonucleotide, harmful substance, immunological stress etc. increase the drainage of nucleotides in the food, all can have influence on requirement, all await further determining.
The research of Beta-carrotene: beta carotene is the antioxidant vitamins bioactivator, has the effect of oxidation and removing free radicals, is to keep the indispensable nutrient of normal physiological function.It is anti-oxidant and strengthen immunologic function, may be that it mainly acts on the basis.Lack or deficiency when food is medium-term and long-term, can cause that metabolic disorder, resistance reduce, easily by bacteria attack, particularly therefore the infant can cause flu, Bronchopneumonia.
According to domestic and foreign literature, beta carotene has tangible immunoprophylaxis and immunization therapy effect.It can protect phagocyte and avoid damage of radicals, strengthens cytophagous phagocytic activity.The oxidation and removing free radicals function that beta carotene had has protective effect to the membrane receptor of macrophage, it is unlikely because of oxidation loses, thereby improved the recognition capability of macrophage to antigen.This shows that the immunoregulation effect of beta carotene is also closely related with its oxidation and removing free radicals effect.Beta carotene has 11 conjugated double bonds, is the most effective singlet oxygen scavenger.
β--carrotene can strengthen the defence capability of body, promotes enhancing body's immunological function.With β--carrotene is used for the prevention and the supplemental treatment of tumour, and good synergism can be arranged.
Summary of the invention
The invention provides a kind of immunity formula milk powder, its raw material comprises following substances in parts by weight: the milk of 7000000-8000000 part, the immunoglobulin (Ig) of 4000-10000 part, the beta carotene of the nucleotides of 269-720 part and 2-3 part.
Nucleotides is made up of the AMP of 40-120 part, GMP, the CMP of 110-300 part, the UMP of 50-170 part and the IMP of 15-40 part of 54-160 part.
Wherein, milk is 7000000 parts, and immunoglobulin (Ig) is 4000 parts, and nucleotides is 269 parts, and beta carotene is 2 parts.
Wherein, milk is 8000000 parts, and immunoglobulin (Ig) is 10000 parts, and nucleotides is 720 parts, and beta carotene is 3 parts.
In addition, raw material also comprises other nutriments.
These other nutriments include lecithin, vanilla, mineral matter, vitamin.
The present invention also provides a kind of preparation method of immunity formula milk powder, its step is as follows: earlier milk is preheated to 60-65 ℃, homogeneous under 180bar again, pasteurization, vacuum concentrate then, add immunoglobulin (Ig), nucleotides and beta carotene again after spray-drying promptly.
After spray-drying, spray other nutriments again and promptly get this immunity formula milk powder.
Milk is meant ordinary milk or the recombined milk that is mixed with milk powder, whey powder, rare cream or other milk ingredients among the present invention.
The present invention is carrier with milk, add the active immne material and (be mainly IGG, IGA IGM), bioactivators such as five kinds of nucleic acids (AMP, GMP, CMP, UMP, IMP) and Beta-carrotene, concentrates and the Powdered dairy products of vacuum spray drying through vacuum.
Immunity formula milk powder has immunological regulation on the basis of balanced and complete nutrient, promote the function of brain and physical growth growth effect, is a kind of humanized formula milk.
Product can adopt and fill nitrogen compound plastic bag and iron flask packaged form, and the local flavor of product, mouthfeel, color and luster, overall assessment etc. all are better than the reference product of being correlated with, and has immunoregulatory effect through the zoopery checking.
The specific embodiment
Embodiment 1:
Composition of raw materials:
| Raw material | Quality |
| Milk | 7000 kilograms |
| Immunoglobulin (Ig) | 4 kilograms |
| Nucleotides: AMP | 40 grams |
| Nucleotides: GMP | 54 grams |
| Nucleotides: CMP | 110 grams |
| Nucleotides: UMP | 50 grams |
| Nucleotides: IMP | 15 grams |
| Carrotene | 2 grams |
Earlier milk is preheated to 60 ℃, homogeneous under 180bar concentrates in 85 ℃ of following sterilizations, vacuum then, adds immunoglobulin (Ig), nucleotides and beta carotene again, and last spray-drying promptly gets final products.
Embodiment 2:
Composition of raw materials
| Raw material | Quality |
| Milk | 7500 kilograms |
| Immunoglobulin (Ig) | 7 grams |
| Nucleotides: AMP | 80 grams |
| Nucleotides: GMP | 100 grams |
| Nucleotides: CMP | 220 grams |
| Nucleotides: UMP | 120 grams |
| Nucleotides: IMP | 30 grams |
| Beta-carrotene | 2.5 gram |
| Lecithin | 2 kilograms |
Earlier milk is preheated to 62 ℃, homogeneous under 180bar concentrates in 88 ℃ of following sterilizations, vacuum then, adds immunoglobulin (Ig), nucleotides and beta carotene again, after the spray-drying again spraying lecithin promptly get final products.
Embodiment 3:
Composition of raw materials:
| Raw material | Quality (kg) |
| Milk | 8000 kilograms |
| Lecithin | 2 kilograms |
| Immunoglobulin (Ig) | 10 kilograms |
| Nucleotides: AMP | 120 grams |
| Nucleotides: GMP | 160 grams |
| Nucleotides: CMP | 300 grams |
| Nucleotides: UMP | 170 grams |
| Nucleotides: IMP | 40 grams |
| Beta-carrotene | 3 grams |
| Vanilla | 2 kilograms |
Earlier milk is preheated to 65 ℃, homogeneous under 180bar, then 95 ℃ of following sterilizations, vacuum concentrates, and adds immunoglobulin (Ig), nucleotides and beta carotene again, and spraying lecithin and vanilla promptly get final products again after the spray-drying.
Above-mentioned AMP, GMP, CMP, UMP and IMP are meant adenine ribonucleotide, guanosint ribotide, cytidylic acid, uridylate, hypoxanthine ribonucleotide respectively.
Raw material sources are given an example:
Milk: Shanghai Bright Dairy ﹠ Food Co., Ltd the 7th, nine pastures
New Zealand's dairy starting material (China) Co., Ltd
U.S. PROLIANT company
Immunoglobulin (Ig): the good Dairy Company of Australian Australia scholar
Nucleotides: Japanese YAMASA company
Carrotene: German BASF AG
Application Example:
The functional evaluation of immunity formula milk powder (with the product of embodiment 1 as given the test agent)
Immunoregulation effect is meant a species specificity physiological reaction of human body contact antigenicity foreign matter or dissident's composition, and it is the identification self that human body obtains in the process of evolving, a kind of important physiological function of excluding outsiders.About strengthening the health food of immunoregulation effect, should be meant that those can strengthen body to the resistance of disease, anti-infective, the antitumor and food of keeping self physiological equilibrium.In one group of experiment, sample has humidification and to other experiment unrestraint effects, can judge that promptly this given the test agent has the immunoregulation effect of this aspect the experiment of immune system each side function.If the Function detection that (contains two) more than two positive is as a result arranged in cellular immune function, humoral immune function, monokaryon-macrophage function and NK cell function detect, can judge that promptly this is tried thing and has immunoregulation effect.
Experiment material:
Sample: 1# immunity formula milk powder, 2# ordinary powdered milk
Provide by Shanghai Bright Dairy ﹠ Food Co., Ltd. technique center.
Animal used as test and feeding patterns:
Animal used as test, mouse be available from Jiangsu Province's atomic energy Institute for Medical Research Animal House, Kunming mouse, 6 ages in week, body weight 20 ± 2g, ♀ ♂ dual-purpose.Mouse is divided into two at random to be organized greatly:
The A group is normal group;
The B group is the immunocompromised group through cobalt-60 irradiation.
Be divided into three groups at random with each big group subsequently, feed to formula milk for one group, feed for one group and give normal milk powder, feed to drinking water for one group.The equal normal daily ration of freely ingesting of all animals.30 days nursing time is in experiment fasting the previous day.
Experimental technique:
" the functional evaluation method of health food " with reference to the promulgation of health supervision department of the Ministry of Public Health.
Immune function experiment:
1. to mouse immune organ weight's influence
Get normal Kunming mouse random packet, feeding finishes back disconnected neck next day puts to death and dissects, and takes out mouse spleen and thymus gland and weighs, and be calculated as follows spleen index and thymus index.
2. monokaryon-macrophage function is measured--and Turnover of Mouse Peritoneal Macrophages is engulfed the chicken red blood cell test
Kunming mouse, 10～15 every group, the next day behind the last feeding, each organizes lumbar injection concentration is 5 * 10
6The chicken red blood cell suspension that/ml preserves with A Shi liquid (1ml/ only) is collected mouse peritoneal liquid, smear with the physiological saline wash-out behind the 30min at interval.Fix with 1: 1 acetone-formalin, Ji Mu Sa-Wright's staining liquid dyes, and counts in 100 macrophages with oily mirror and engulfs the erythrocytic macrophage number of chicken and engulf the erythrocytic sum of chicken, and calculate percentage phagocytosis and phagocytic index by following formula.
3. the mensuration of cellular immune function--mouse spleen lymphocyte conversion test
Get experiment mice, after feeding finishes, the disconnected neck of mouse is put to death aseptic taking-up spleen, prepare splenocyte suspension respectively, separate with lymphocyte separation medium and obtain lymphocyte, Hanks washing three times, and with trypan blue dyeing liquor dyeing counting, viable count＞95%, adjusting cell concentration is 2 * 10
6/ ml.Divide two groups to add in 24 well culture plates cell suspension, every hole 1ml; One group adds Con A (another group compares, and puts 5%CO for 100ug/ml, Sigma) 50 μ l
2, in 37 ℃ of saturated humidity incubators.Cultivate and finish 4h, every hole is inhaled and is removed supernatant 0.7ml, adds RPMI-1640 (GIBCO) nutrient solution that does not contain calf serum, and (5mg/ml, Sigma) 72h is continued to be cultured in 50 μ l/ holes to add MTT simultaneously.After cultivating end, every hole adds 1ml acid isopropyl alcohol, and the piping and druming mixing is used 721 spectrophotometers, and the 570nm wavelength is measured the OD value.
4. data are handled and result's judgement:
The gained data are all imported computer, and carry out data with electrical form (Microsoft Excel) and handle, and with t check carrying out significance test.P＜0.05 expression significant difference, P＞0.05 expression difference is not remarkable.
Experimental result:
Influence to the mouse immune organ weight:
Spleen and thymus gland are main immune organ in the body, and T lymphocyte and bone-marrow-derived lymphocyte and macrophage are wherein arranged, and with humoral immunity and cellular immunity substantial connection are arranged, and trier makes the spleen weightening finish that its influence to immune organ can tentatively be described.Formula milk sees Table 1 to the influence of mouse spleen and thymus gland.
The comparison of table 1 immune organ weight (x ± s)
| Group | Size of animal before the experiment | Experiment back size of animal | Death rate % | Spleen index mg/g | Significance | Thymus index mg/g | Significance | |
| Normally | Formula milk | 10 | 10 | 0 | 5.50±0.72 | --- | 8.29±0.43 | --- |
| Ordinary powdered milk | 10 | 10 | 0 | 4.84±0.82 | P 1＞0.05 | 7.23±0.56 | P 1＞0.05 | |
| The water contrast | 10 | 10 | 0 | 4.65±0.4 | P 2＞0.05 | 5.73±0.45 | P 2＜0.05 | |
| Immunocompromised | Formula milk | 15 | 13 | 13 | 4.95±0.36 | --- | 6.18±0.4 | --- |
| Ordinary powdered milk | 15 | 8 | 47 | 4.40±0.65 | P 1＞0.05 | 5.17±0.4 | P 1＞0.05 | |
| The water contrast | 15 | 9 | 40 | 4.1±0.57 | P 2＞0.05 | 5.1±0.3 | P2＞0.05 | |
Annotate: P
1Be formula milk and ordinary powdered milk comparison; P
2For formula milk and water are compared
By table 1 as seen, formula milk has the trend that promotes its growth to the effect of spleen and thymus gland.The index and spleen index of normal experimental group is compared with the experimental group of feeding ordinary powdered milk experimental group and feeding drinking water slightly with thymus index and is improved after the feeding formula milk powder; But except that the thymus index of feeding formula milk powder group and feeding drinking water group has on statistical significance the evident difference, the spleen index and the thymus index of formula milk group and ordinary powdered milk group do not have significant difference on statistical significance.
Its spleen index and thymus index also have growth trend and return to normal level behind the immunocompromised group mouse feeding formula milk behind the irradiation.Compare its spleen index with feeding ordinary powdered milk group on the same group and thymus index does not all have significant difference.
But the survival rate that the mouse feeding formula milk after experiment is found to be subjected to γ-radiation exposure is a group is obviously than the survival rate height of the experimental group of feeding ordinary powdered milk group and drinking water.The death rate of the mouse of feeding formula milk powder between feeding period is 13%, and the death rate of feeding ordinary powdered milk is 47%, and the death rate of drinking water is 40%.Deducibility thus, behind the formula milk of ingesting, immunoglobulin (Ig) wherein impels mouse to recover immunocompetence, has improved the immunity of body, thereby has prolonged its life span.
The experiment of Turnover of Mouse Peritoneal Macrophages phagocytic function:
Turnover of Mouse Peritoneal Macrophages (M φ) is engulfed the quantity of chicken red blood cell to formula milk and the influence of activate the phagocytic capacity sees Table 2.
The influence of table 2 pair Turnover of Mouse Peritoneal Macrophages phagocytic function (x ± s)
| Group | The animal used as test number | Percentage phagocytosis | Significance | Phagocytic index | Significance | |
| Normally | Formula milk | 10 | 50.40±6.03 | -- | 0.92±0.04 | -- |
| Ordinary powdered milk | 10 | 35.60±6.20 | P 1＜0.05 | 0.84±0.03 | P 1＜0.05 | |
| The water contrast | 10 | 32.00±3.06 | P 2＜0.05 | 0.77±0.06 | P 2＜0.05 | |
| Immunocompromised | Formula milk | 10 | 35.60±4.76 | -- | 0.73±0.04 | -- |
| Ordinary powdered milk | 8 | 25.66±2.92 | P 1＜0.05 | 0.65±0.06 | P 1＜0.05 | |
| The water contrast | 9 | 23.20±2.94 | P 2＜0.05 | 0.65±0.07 | P 2＜0.05 | |
Annotate: P
1Be formula milk and ordinary powdered milk comparison; P
2For formula milk and water are compared
Macrophage is the key cells of immunity of organism, it is bearing picked-up, handling and is offering the critical function of antigen, the action effect cell is participated in immunologic process directly again simultaneously, measures the phagocytic function of macrophage, can understand the immune state of body to a certain extent.
Table 2 is the result show, normal mouse is after feeding formula milk powder, the ability of its macrophage phagocytic chicken red blood cell has been compared conspicuousness and has been improved with control group, the cell that can engulf chicken red blood cell in per 100 macrophages is increased to 50.4 from 35.6, has improved 42%; Engulf the quantity of chicken red blood cell in per 100 macrophages and bring up to 92, improved 10% by 84.And the experimental group of feeding ordinary powdered milk does not change aspect the activated mononuclear macrophage.
In the experimental group of immunocompromised, mouse is after feeding formula milk powder, and the feeding ordinary powdered milk in its phagocytic index and percentage phagocytosis and the same experimental group is compared all and is significantly increased with two groups that drink distilled water, and returns to normal level basically.Show that formula milk can make the phagocytic function of monokaryon-macrophage of the mouse of immunocompromised return to normal level.
This experimental result explanation formula milk has significant humidification to the non-specific immune function of mouse.
The lymphocyte transformation experiment
Table 3 lymphocyte transformation significance test (x ± s)
| Group | The animal used as test number | 1# | 2# | 1#-2# | Significance | |
| OD570nm+ConA | OD570nm | |||||
| Normally | Formula milk | 10 | 0.274±0.1 | 0.198±0.12 | 0.076 | --- |
| Ordinary powdered milk | 10 | 0.134±0.07 | 0.104±0.06 | 0.030 | P 1＜0.05 | |
| The water contrast | 10 | 0.154±0.05 | 0.123±0.03 | 0.031 | P 2＜0.05 | |
| Immunocompromised | Formula milk | 10 | 0.182±0.06 | 0.115±0.02 | 0.067 | --- |
| The milk powder contrast | 8 | 0.104±0.06 | 0.086±0.05 | 0.018 | P 1＜0.05 | |
| The water contrast | 9 | 0.100±0.02 | 0.080±0.02 | 0.02 | P 2＜0.05 | |
Annotate: P
1Be formula milk and ordinary powdered milk comparison; P
2For formula milk and water are compared
Spleen is the filtration organ in the blood access, also is immune organ maximum in the body, can produce a large amount of lymphocytes.Lymphocyte is the most complicated in a body clone, mainly comprises T cell, B cell, NK cell and K cell etc.The spleen medium size lymphocyte accounts for 60% of sum based on the B cell.The B cell is brought into play its humoral immune function by producing antibody.
By table 3 as seen, normal mouse its lymphocytic conversion ratio after feeding formula milk powder (p＜0.05=that all is significantly increased that compares with ordinary powdered milk, strengthened the activity of bone-marrow-derived lymphocyte in the spleen significantly, and ordinary powdered milk does not have evident difference with the experimental group of drinking distilled water on this performance, shows the effect that formula milk has certain promotion bone-marrow-derived lymphocyte to transform.
The experimental group of immunologic hypofunction its lymphocyte conversion ratio after feeding formula milk powder also returns to normal level.
This experimental result proof formula milk is positive to the mouse cell results of immunologic detection, has illustrated that formula milk has the adjusting effect aspect cellular immunity.
Conclusion
Formula milk does not have remarkable influence to normal mouse immune organ weight.But the immune organ weight of immunocompromised mouse can be returned to normally.
Formula milk is measured the monokaryon-macrophage function of normal mouse and is positive, and monokaryon-macrophage function of immunocompromised mouse can be returned to normal level.
Formula milk is positive to the cellular immune function assay of normal mouse, and the cellular immune function of immunocompromised mouse can be returned to normal level.
In view of above experimental result,, show that the sample " immunity formula milk powder " that Shanghai Bright Dairy ﹠ Food Co., Ltd. provides has the immunocompetent function of enhancing according to " the health food function assessment assessment process and the method for inspection " of health supervision department of Ministry of Public Health promulgation.
Claims (7)
1, a kind of immunity formula milk powder, its raw material comprises following substances in parts by weight: the milk of 7000000-8000000 part, the immunoglobulin (Ig) of 4000-10000 part, the beta carotene of the nucleotides of 269-720 part and 2-3 part, the milk of above-mentioned weight portion is preheated to 60-65 ℃, homogeneous under 180bar again, pasteurization, vacuum concentrate then, add immunoglobulin (Ig), nucleotides and the beta carotene of above-mentioned weight portion again after spray-drying promptly.
2, immunity formula milk powder according to claim 1 is characterized in that this nucleotides is made up of the AMP of 40-120 part, GMP, the CMP of 110-300 part, the UMP of 50-170 part and the IMP of 15-40 part of 54-160 part.
3, immunity formula milk powder according to claim 1 is characterized in that this milk is 7000000 parts, and immunoglobulin (Ig) is 4000 parts, and nucleotides is 269 parts, and beta carotene is 2 parts.
4, immunity formula milk powder according to claim 1 is characterized in that this milk is 8000000 parts, and immunoglobulin (Ig) is 10000 parts, and nucleotides is 720 parts, and beta carotene is 3 parts.
5, immunity formula milk powder according to claim 1 is characterized in that this raw material also comprises other nutriments, and these other nutriments include lecithin, vanilla, mineral matter, vitamin.
6, the preparation method of claim 1,2,3,4 or 5 described immunity formula milk powders, its step is as follows: earlier milk is preheated to 60-65 ℃, homogeneous under 180bar again, pasteurization, vacuum concentrate then, add immunoglobulin (Ig), nucleotides and beta carotene again after spray-drying promptly.
7, the preparation method of immunity formula milk powder according to claim 6 is characterized in that spraying other nutriments again and promptly gets this immunity formula milk powder after spray-drying, these other nutriments include lecithin, vanilla, mineral matter, vitamin.
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| CN101953403B (en) * | 2010-09-25 | 2012-05-23 | 澳优乳业（中国）有限公司 | Infant formula milk powder added with secretory immunoglobulin A and preparation method thereof |
| CN102986877B (en) * | 2011-09-14 | 2014-07-23 | 光明乳业股份有限公司 | Production technology for formula milk powder |
| CN103598353B (en) * | 2013-11-07 | 2015-11-18 | 河北三元食品有限公司 | Breast base lactose-free baby formula powder |
-
2002
- 2002-11-13 CN CN 02145269 patent/CN1212068C/en not_active Expired - Lifetime
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| Publication number | Publication date |
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| CN1465261A (en) | 2004-01-07 |
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Address after: 201103 Minhang District, Wuzhong Road, No. 578, Patentee after: BRIGHT DAIRY & FOOD Co.,Ltd. Address before: 201103 Minhang District, Wuzhong Road, No. 578, Patentee before: Shanghai Bright Dairy & Food Co.,Ltd. |
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Granted publication date: 20050727 |