CN121127501A - 锌指蛋白、锌指核酸酶、载体和应用它们的基因组编辑方法 - Google Patents

锌指蛋白、锌指核酸酶、载体和应用它们的基因组编辑方法

Info

Publication number
CN121127501A
CN121127501A CN202480032441.1A CN202480032441A CN121127501A CN 121127501 A CN121127501 A CN 121127501A CN 202480032441 A CN202480032441 A CN 202480032441A CN 121127501 A CN121127501 A CN 121127501A
Authority
CN
China
Prior art keywords
nls
sequence
zinc finger
zfp
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202480032441.1A
Other languages
English (en)
Chinese (zh)
Inventor
大西晓士
安田和史
石丸蓝子
佐久间哲史
山本卓
野村涉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Weisai Gene Technology Co ltd
Hiroshima University NUC
Original Assignee
Weisai Gene Technology Co ltd
Hiroshima University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Weisai Gene Technology Co ltd, Hiroshima University NUC filed Critical Weisai Gene Technology Co ltd
Publication of CN121127501A publication Critical patent/CN121127501A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN202480032441.1A 2023-06-16 2024-06-14 锌指蛋白、锌指核酸酶、载体和应用它们的基因组编辑方法 Pending CN121127501A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2023098848 2023-06-16
JP2023-098848 2023-06-16
PCT/JP2024/021599 WO2024257843A1 (ja) 2023-06-16 2024-06-14 ジンクフィンガータンパク質、ジンクフィンガーヌクレアーゼ、ベクター並びにこれらを用いたゲノム編集方法

Publications (1)

Publication Number Publication Date
CN121127501A true CN121127501A (zh) 2025-12-12

Family

ID=93852226

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202480032441.1A Pending CN121127501A (zh) 2023-06-16 2024-06-14 锌指蛋白、锌指核酸酶、载体和应用它们的基因组编辑方法

Country Status (5)

Country Link
EP (1) EP4729547A1 (https=)
JP (1) JPWO2024257843A1 (https=)
CN (1) CN121127501A (https=)
AU (1) AU2024302819A1 (https=)
WO (1) WO2024257843A1 (https=)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019060631A1 (en) * 2017-09-20 2019-03-28 Helix Nanotechnologies, Inc. EXPRESSION SYSTEMS THAT FACILITATE THE DELIVERY OF NUCLEIC ACIDS AND METHODS OF USE
MX2021002250A (es) 2018-08-27 2021-05-27 Univ Hiroshima Dominio de nucleasa novedoso y uso del mismo.
EP3980533A1 (en) * 2019-06-07 2022-04-13 Scribe Therapeutics Inc. Engineered casx systems
EP4405490A2 (en) * 2021-09-23 2024-07-31 Scribe Therapeutics Inc. Self-inactivating vectors for gene editing
CN115716880A (zh) * 2022-12-07 2023-02-28 云舟生物科技(广州)股份有限公司 一种核定位荧光蛋白及其应用

Also Published As

Publication number Publication date
WO2024257843A1 (ja) 2024-12-19
EP4729547A1 (en) 2026-04-22
AU2024302819A1 (en) 2026-01-22
JPWO2024257843A1 (https=) 2024-12-19

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