CN1209158C - Lung clearing oral liquid preparation for treating viral pneumonia in Children and preparing method thereof - Google Patents
Lung clearing oral liquid preparation for treating viral pneumonia in Children and preparing method thereof Download PDFInfo
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Abstract
The present invention discloses an oral lung-heat clearing liquid preparation for treating child virus pneumonia, which is prepared from the Chinese herbal medicines of prepared herba ephedrae, gypsum, bitter apricot seed, pepperweed seed, white mulberry root-bark, hogfenneL root, stiff silkorm, red sage root, giant knotweed rhizome, bistort rhizome, etc. A preparing method comprises: grinding the bitter apricot seed, and distilling the powder to obtain distillate; mixing the rest medicines with bitter apricot seed dregs, decocting the mixture with water twice, concentrating, and depositing the mixture in alcohol; filtering to obtain a supernatant liquid, recovering ethanol, adding a right amount of water, boiling and filtering to obtain a filter liquid for use; mixing a sucrose solution with the bitter apricot seed distillate and extracting solutions of ephedra and the like, adding water to 90 ml, packaging and sterilizing the medicinal liquid. The oral lung-heat clearing liquid preparation has the functions for promoting the dispersing function of lung, clearing heat, eliminating phlegm, detoxicating and promoting blood circulation, and has significant curative effect on the syndrome of phlegm-heat obstructing lung of the child virus pneumonia. Experiments show that the oral lung-heat clearing liquid preparation has the efficacy for relieving fever, relieving cough, resolving phlegm, resisting viruses, relieving lung pathological changes caused by virus pneumonia, adjusting the immune functions of human bodies (such as inducing interleukin-2, gamma interferon), etc. The oral lung-heat clearing liquid preparation is an effective medicament for treating the syndrome of phlegm-heat obstructing lung of child virus pneumonia.
Description
One, technical field
The present invention relates to the field of Chinese medicines, specifically relating to Chinese herbal medicine is a kind of compound preparation and preparation method for the treatment of infantile viral pneumonia that raw material prepares.
Two, background technology
Infantile pneumonia is the common disease of department of pediatrics, and World Health Organization (WHO) has classified primary disease as one of three kinds of important pediatric diseases in the whole world, and the Chinese government also classifies it as one of four kinds of diseases of department of pediatrics keypoint control.It is reported that policlinic department of pediatrics lesion inpatient's 1/4～1/2 is children pneumonia at present, County Hospital then accounts for more than 1/2.According to World Health Organization's statistics, the whole world annual child below 5 years old about 400 die ten thousand deaths in pneumonia, and wherein the overwhelming majority is the child of developing country, has 2/3 to be infant.In China, there are every year about 300,000 children below 5 years old to die of pneumonia approximately, case fatality rate accounts for first of infant death reason, illustrates that primary disease is one of disease of present serious harm children's health.Therefore, active prevention and treatment infantile pneumonia are studied new Therapeutic Method, have crucial meaning.
At present, in the paathogenic factor of China's infantile pneumonia, viral infection accounts for about 50%.And modern medicine is used for the treatment of medicine for treating viral infections such as ribavirin etc., and curative effect is unsatisfactory, have in recent years report use interferon (Zhang Xingquan. low dose of interferon therapy infantile viral pneumonia. Henan pharmaceutical information, 2002; 10 (22): 50), ribavirin (Ma Jinhai is etc. interferon, virazole, SHUANGHUANLIAN treatment infantile viral pneumonia observation of curative effect for Qiu Yinping, Bian Guangbo. department of pediatrics pharmaceutical journal, 1999; 5 (1): 28～30) treatment infantile viral pneumonia, obtain curative effect preferably, various bibliographical informations are also a lot.And these researchs lack the experimentation data of nosetiology diagnosis basis and system mostly, though some research is comparatively comprehensive, but being the research summary to certain single method of treatment mostly, is the effective therapy and the deep research of shortage system still of square medicine of main treatment infantile viral pneumonia with the determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs.Chinese medicine commonly used clinically at present such as Zedoary turmeric oil glucose injection (Jia Jingwei, Hu Jiahui. Zedoary turmeric oil glucose injection treatment infantile viral pneumonia 21 examples are observed. Heilungkiang medical science, 1999; 22 (1): 92, Andrographolide (nourish and be, study of the Song Dynasty a tree, used in making timber for boats, Zhang Gonghui is etc. the curative effect of Andrographolide ribavirin therapy infantile viral pneumonia relatively. Chinese Journal of New Drugs and Clinical Remedies, 1999; 8 (1): 55～56) etc., its mechanism of action mainly is blood circulation promoting and blood stasis dispelling, promotes the blood circulation of pulmonary, and the absorption that helps inflammation is dissipated, but does not have therapeutical effect such as tangible antiviral; In addition as set upright antitoxin syrup (Zhu Lina, Li Qiuying. set upright antitoxin syrup treatment infantile viral pneumonia 69 examples. Beijing University of Chinese Medicine's journal, 1995; 8 (3): 50～51), lung heat clearing drink (Chen Yuqin, Shen Hongxia. the 263 routine clinical observations of lung heat clearing drink treatment infantile viral pneumonia. Henan Chinese medicine academic periodical, 2000; 15 (5): 56～57), Rhizoma Phragmitis five brave granules (Xiong Jibai. the clinical research of the brave granules treatment of Rhizoma Phragmitis five viral pneumonias. Jilin Chinese medicine, 1995; (3): 6), do not form new drug as yet and be widely used in clinical though the therapeutical effect of viral pneumonia is had some researchs; And the therapeutical effect of Chinese medicine Herba Houttuyniae injectio etc. only limits to heat-clearing and toxic substances removing.The traditional Chinese medical herbal treatment infantile viral pneumonia is still based on decoction clinically.We are through the clinical observations of decades research, think the cause of disease of infantile viral pneumonia: interior because deficiency of vital QI, weakened defensive QI; Outer because the evil poison of diseases caused by exogenous pathogenic factor pathogenic wind-warm.Though can be divided into a plurality of pattern of syndrome, we show that about 80% belongs to syndrome of phlegm-heat obstructing lung after having added up the first department of pediatrics pneumonia inpatient of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, and promptly expectorant heat is stopped up resistance, the lung qi obturation is the main pathogenesis of primary disease.Therefore, its main method of treatment should be clearing and dissipating phlegm-heat, lung qi dispersing and opens and closes, and simultaneously according to the cause of disease (the evil poison of pathogenic wind-warm) of primary disease and have blood stasis sign concurrently in this course of disease, should help the method for invigorating blood circulation with detoxifcation more.Because the child administration Chinese medicine decoction is difficulty comparatively, so development is fit to child's characteristics as early as possible, the Chinese formulated products of treatment infantile viral pneumonia is the important topic that the department of pediatrics worker faces.
Three, summary of the invention
1, goal of the invention
The present invention seeks to adopt lung qi dispersing switching, removing heat-phlegm and detoxifcation invigorate blood circulation drug matching, preparation, providing has tangible antiviral, anti-inflammation, spasmolytic relieving cough and asthma, improves pulmonary's blood circulation the viral pneumonia infant, the Chinese traditional compound medicine of effect such as human body immunity improving power.This medicine is a kind of viral pneumonia syndrome of phlegm-heat obstructing lung infant that not only can be used for, and also can be used for the natural Chinese medicines preparation of acute bronchitis phlegm-heat cough card infant.Another object of the present invention provides the preparation method of this medicine.
2, technical scheme
(1) the oral liquid for clearing away lung-heat crude drug is formed and weight proportion
A kind of oral liquid for clearing away lung-heat preparation for the treatment of infantile viral pneumonia, it is characterized in that being formed: Herba Ephedrae (processed with honey) 3～5g by following bulk drugs and weight proportion, Semen Armeniacae Amarum 8～12g, Gypsum Fibrosum 20～40g, Semen Lepidii (Semen Descurainiae) 5～10g, Cortex Mori (processed with honey) 8～12g, Radix Peucedani 8～12g, Bombyx Batryticatus (system) 5～8g, Radix Salviae Miltiorrhizae 5～8g, Rhizoma Polygoni Cuspidati 10～15g, Rhizoma Bistortae 10～15g.
If heavier at the infant lung-heat syndrome, the expectorant blood stasis syndrome resembles under the lighter situation, can add Radix Scutellariae 6～10g in crude drug, adds adjuvant stevioside, multitudinous sugar, potassium sorbate again.Be Herba Ephedrae (processed with honey) 3～5g, Semen Armeniacae Amarum 8～12g, Gypsum Fibrosum 20～40g, Semen Lepidii (Semen Descurainiae) 5～10g, Cortex Mori (processed with honey) 8～12g, Radix Peucedani 8～12g, Rhizoma Polygoni Cuspidati 10～15g, Radix Scutellariae 6～10g.
(2) side separates
Side's epheday intermedia, hot, warm, relieving the exterior syndrome by diaphoresis, depressed lung-energy dispersing, Shennong's Herbal are said " main apoplexy cold headache, pyrexial malaria is delivered perspiration, the gas that uncharms ends cough with dyspnea, except that cold and heat ", are the key medicine of inducing sweat and relievining asthma; Semen Armeniacae Amarum, bitter, cold, relieving cough and asthma, Compendium of Material Medica is said " Semen Armeniacae Amarum can loose and can fall, the wind sending down the abnormal ascending QI so expelling pathogenic factors from muscles looses ", " card is controlled secret formula " carries " cough-relieving is coughed, the expectorant lung moistening "; Gypsum Fibrosum, sweet cold, clearing away heat-fire, relieving restlessness cough-relieving, the curing mainly of Shennong's Herbal meaning " apoplexy cold and heat, heart subinverse gas is frightened breathes heavily the xerostomia scorching tongue ", " Mingyi Bielu " replenished function " expelling pathogenic factors from muscles by diaphoresis " again and cured mainly " seasonal epidemic pathogens headache fever of the body, the big heat of three warmers, skin heat ".Three medicines are shared to be principal agent, and that suits is the meaning of famous ancient prescription treatise on Febrile Diseases MAXINGSHIGAN TANG.Radix Peucedani, is slightly cold at toil, the function lowering the adverse-rising QI to resolve phlegm, and dispelling wind-heat, Compendium of Material Medica is called its " clearing away lung-heat, the heat of reducing phlegm, the ailment said due to cold or exposure of loosing ".Cortex Mori, sweet, cold, the function eliminating pathogen from the lung for relieving asthma, " property of medicine opinion " meaning " controlling the lung qi dyspnea with fullness of the chest ", Compendium of Material Medica propose " eliminating the pathogens from the lung, sending down the abnormal ascending QI, diffusing blood " again.Semen Lepidii (Semen Descurainiae), toil, cold, the function eliminating pathogen from the lung for relieving asthma, " Kaibao Bencao " calls its " treat lung and stop up upward gas cough, Dingchuan is short, removes phlegm retention in the heart ".Rhizoma Polygoni Cuspidati, bitter cold, function detoxifcation, blood circulation promoting and blood stasis dispelling, preventing phlegm from forming and stopping coughing, " property of medicine opinion " meaning " controlling big hot excessive thirst ... press all pyretic toxicity ".Radix Peucedani, Cortex Mori, Semen Lepidii (Semen Descurainiae), Rhizoma Polygoni Cuspidati share and are ministerial drug, have lowering the adverse-rising QI to resolve phlegm, the merit that detoxifcation is invigorated blood circulation.Add again that Bombyx Batryticatus reduces phlegm, Radix Salviae Miltiorrhizae is invigorated blood circulation, the Rhizoma Bistortae clearing away lung-heat to relieve cough is relievingd asthma, be adjuvant.In a word, oral liquid for clearing away lung-heat is on the basis of tradition prescription MAXINGSHIGAN TANG, selected side's medicine, and, add with opening the product that lung qi dispersing gas, clearing away phlegm detoxifcation are invigorated blood circulation, forming and have lung qi dispersing and open and close at the pathogenic characteristic of infantile viral pneumonia syndrome of phlegm-heat obstructing lung, removing heat-phlegm, the prescription of the detoxifcation effect of invigorating blood circulation.
We adopt lung qi dispersing to open and close, removing heat-phlegm, the new method that detoxifcation is invigorated blood circulation, according to the dialectical syndrome of phlegm-heat obstructing lung that mostly is greatly of the characteristics of infantile viral pneumonia, and have the card of blood stasis and pyretic toxicity concurrently, reopen lung qi dispersing gas so on the basis of dispersing lung-QI and dissipating phlegm, add, the medicine that assistant is invigorated blood circulation with detoxifcation.
A kind of preparation method for the treatment of the oral liquid for clearing away lung-heat of infantile viral pneumonia, its preparation process is:
(1) weigh and distill after Semen Armeniacae Amarum 8～12g pulverizes, collect its distillate, standby;
(2) with the Herba Ephedrae (processed with honey) of described weight proportion, Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Bombyx Batryticatus (system), Radix Salviae Miltiorrhizae, Rhizoma Polygoni Cuspidati, Rhizoma Bistortae 9 flavor medicines and Semen Armeniacae Amarum medicinal residues merge, the decocting secondary, add for the first time 5～10 times of amounts of water, behind the merceration 30～60 minutes, decocted 1～1.5 hour, add 8～10 times of amounts of water for the second time, decocted 0.5～1 hour, behind the decocting secondary, the secondary medicinal liquid merges, and is concentrated into relative density 1.05～1.15 under 60～80 ℃, add ethanol and reach 60%～70% to containing the alcohol amount, staticly settled 24～36 hours, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, it is an amount of to add water, boil, cold preservation 24～48 hours is filtered drug extract standby;
(3) get sucrose 6～8g, it is an amount of to add water, and heating for dissolving is prepared into sucrose solution, filters, standby;
(4) merge drug extract, sucrose solutions such as Semen Armeniacae Amarum distillate, Herba Ephedrae, add water move to 90ml, fill, circulation steam sterilization 30 minutes and making.
If in crude drug, also add Radix Scutellariae, add adjuvant stevioside, sucrose, potassium sorbate again, the preparation method of oral liquid for clearing away lung-heat of the present invention then, its preparation process is:
(1) Herba Ephedrae (processed), Gypsum Fibrosum, Semen Armeniacae Amarum, Semen Lepidii (Semen Descurainiae), Radix Scutellariae, Cortex Mori, Radix Peucedani, the Rhizoma Polygoni Cuspidati 8 flavor medicines with described weight proportion decoct with water secondary, 10 times of water gagings for the first time, decocted 1.5 hours, 8 times of water gagings for the second time, decocted 1.0 hours, the secondary medicinal liquid merges the back and filters, medicinal liquid is concentrated into 1.0g/ml, selecting molecular weight for use is that 10000 ps hollow fiber uf membrane assembly carries out loop ultrafiltration in the cross-flow mode, filtrate decompression concentrates (0.85Mpa, 65～70 ℃) to relative density be 1.05～1.08 (50～60 ℃ of surveys), place and to be cooled to below 40 ℃.
(2) slowly add ethanol and make and contain alcohol amount and reach 70%, fully stir, left standstill 12 hours.
(3) filter, decompression filtrate recycling ethanol adds water to 85ml to there not being the alcohol flavor, boils 10 minutes, leaves standstill 12 hours.
(4) filter, it is 1-2g, sucrose 5-15g, potassium sorbate 0.1-0.3g that filtrate adds stevioside, and adds water to 90ml, stirs evenly, filtration, embedding, and sterilization makes oral liquid for clearing away lung-heat.
Before preparation, select for use ethanol precipitation, high speed centrifugation and flocculate and clarify technology to make with extra care oral liquid for clearing away lung-heat by orthogonal test.
3, beneficial effect
Oral liquid for clearing away lung-heat of the present invention is compared with existing Chinese medicine, have and open lung qi dispersing gas, the clearing away phlegm detoxifcation effect of invigorating blood circulation significantly, there are tangible antiviral, anti-inflammation, spasmolytic to relieving asthma to infantile viral pneumonia syndrome of phlegm-heat obstructing lung infant, improve pulmonary's blood circulation, regulate effects such as immunologic function, tangible antitussive, analgesic, phlegm-dispelling functions have also been pointed out in zoopery, and common Respirovirus is had the obvious suppression effect.Oral liquid for clearing away lung-heat is not only applicable to infantile viral pneumonia acute stage infant, also can be used for the infantile bronchitis card and belongs to the hot person of expectorant.Chinese formulated products is more suitable in child administration, and the preparation technology of oral liquid for clearing away lung-heat is comparatively advanced.
Below can further specify beneficial effect of the present invention with animal experiment and clinical trial in conjunction with the embodiments.
Four, the specific embodiment
Embodiment 1, the composition of oral liquid for clearing away lung-heat crude drug and weight proportion
The composition of oral liquid for clearing away lung-heat preparation raw material medicine and weight proportion: Herba Ephedrae (processed with honey) 3g, Semen Armeniacae Amarum 10g, Gypsum Fibrosum 20g, Semen Lepidii (Semen Descurainiae) 5g, Cortex Mori (processed with honey) 10g, Radix Peucedani 10g, Bombyx Batryticatus (system) 5g, Radix Salviae Miltiorrhizae 5g, Rhizoma Polygoni Cuspidati 12g, Rhizoma Bistortae 12g.
If the infant lung-heat syndrome is heavier, the expectorant blood stasis syndrome resembles lighter, can add Radix Scutellariae 6g and adjuvant stevioside, sucrose in crude drug, potassium sorbate promptly gets oral liquid for clearing away lung-heat.
Embodiment 2, the preparation method of oral liquid for clearing away lung-heat, and its preparation process is:
(1) distill after Semen Armeniacae Amarum 10g pulverizes, collect its distillate, standby;
(2) with Herba Ephedrae (processed with honey) 3g, Gypsum Fibrosum 20g, Semen Lepidii (Semen Descurainiae) 5g, Cortex Mori (processed with honey) 10g, Radix Peucedani 10g, Bombyx Batryticatus (system) 5g, Radix Salviae Miltiorrhizae 5g, water decocted after Rhizoma Polygoni Cuspidati 12g, medicines such as Rhizoma Bistortae 12g merged the Semen Armeniacae Amarum medicinal residues, for the first time 10 times of water gagings, decocted 1.5 hours, 8 times of water gagings decocted 1.0 hours for the second time, and the secondary medicinal liquid merges, 80 ℃ are concentrated into relative density 1.10, add ethanol and reach 60%, staticly settled the leaching supernatant 36 hours to containing the alcohol amount, it is an amount of that recovery ethanol to nothing alcohol flavor adds water, boil, cold preservation 48 hours is filtered drug extract standby;
(3) getting sucrose 7g, to add water an amount of, and heating for dissolving is prepared into sucrose solution, filters, standby;
(4) merge drug extract and sucrose solutions such as Semen Armeniacae Amarum distillate, Herba Ephedrae (processed), add water move to 90ml, fill, sterilization 30 minutes under the circulation steam and making.
If add Radix Scutellariae 6g and adjuvant stevioside, sucrose, potassium sorbate then the preparation method of oral liquid for clearing away lung-heat of the present invention in crude drug, its preparation process is:
(1) weighs Herba Ephedrae (processed), Gypsum Fibrosum, Semen Armeniacae Amarum, Semen Lepidii (Semen Descurainiae), Radix Scutellariae, Cortex Mori, Radix Peucedani, Rhizoma Polygoni Cuspidati and decoct with water secondary, 10 times of water gagings for the first time, decocted 1.5 hours, 8 times of water gagings decocted 1.0 hours for the second time, the secondary medicinal liquid merges the back and filters, medicinal liquid is concentrated into 1.0g/ml, and selecting molecular weight for use is that 10000 ps hollow fiber uf membrane assembly carries out loop ultrafiltration in the cross-flow mode, and filtrate decompression concentrates (0.85Mpa, 65 ℃) to relative density 1.05 (50 ℃ of surveys), put and be chilled to 38 ℃;
(2) slowly add ethanol and make and contain alcohol amount and reach 70%, fully stir, left standstill 12 hours;
(3) filter, decompression filtrate recycling ethanol adds water to 85ml to there not being the alcohol flavor, boils 10 minutes, leaves standstill 12 hours;
(4) filter, filtrate adds stevioside 1.5g, sucrose 10g, potassium sorbate 0.2g, and adds water to 90ml, stirs evenly, filtration, embedding, and sterilization makes oral liquid for clearing away lung-heat;
Before preparation, selected for use ethanol precipitation, high speed centrifugation and flocculate and clarify technology to make with extra care oral liquid for clearing away lung-heat by orthogonal test.
Embodiment 3, the pharmacodynamics zoopery
(1) oral liquid for clearing away lung-heat cough-relieving experimentation
1, material
1.1 equipment iron stand, screw clamp, water manometer, ammonia container (500ml wide mouthed bottle), animal container, spent acid container, electric suction apparatus, perforated plastic pipe, water bath with thermostatic control.
1.2 the ammonia 200ml of reagent 12.5%-25% (Baoying County, Jiangsu chemical reagent factory produces, lot number 970615)
1.3 be subjected to reagent thing oral liquid for clearing away lung-heat (medicine is made up of Herba Ephedrae (processed), Semen Armeniacae Amarum, Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Radix Salviae Miltiorrhizae, Rhizoma Bistortae, Bombyx Batryticatus, Rhizoma Polygoni Cuspidati), every dose is made concentration is 100% oral liquid 90ml.(providing) by first Pharmacy department of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, codeine phosphate 15mg/ sheet (being dissolved into 2mg/ml) with water for injection
1.4 animal Kunming mouse (body weight 18-22g, male and female half and half.Provide by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center).
2, experimental technique and result
2.1 with 48 of mices, be divided into 4 groups at random, 12 every group by body weight.First group is the blank group, and second group is oral liquid for clearing away lung-heat low dose group (20g/Kg bw), and the 3rd group is that the 4th group of oral liquid for clearing away lung-heat high dose group (40g/Kg bw) is codeine phosphate group (30mg/Kg bw).
2.2 give normal saline for first group, second group, the 3rd group low to oral liquid for clearing away lung-heat respectively, high dose, first three groups is taked gastric infusion, the 4th group of lumbar injection codeine, behind the gastric infusion 1 hour, half an hour behind the lumbar injection, beginning to accept aerosol stimulated.
2.3 mice to be measured is put into little rat holder, place the animal container to start suction pump, making mice accept ammonia stimulated to the scheduled time, closed suction pump, take out mice immediately, observe the number of times of coughing in 1 minute (occur more than 3 times typical case's cough action can be regarded as cough).
2.4 ask the effective principle of half by purgation on the statistics, change the time that every mice is accepted spraying, whether the observation cough occurs and writes down it.
2.5 experimental result sees the following form
Table 1 oral liquid for clearing away lung-heat is to the antitussive action of mice
Group dosage (g/Kg bw) number of animals route of administration EDT50 R value
Blank group 20ml 12 ig 18.84s
Lung heat clearing low dose group 20 12 ig 25.12s 133.3%
Lung heat clearing high dose group 40 12 ig 27.12s 143.9%
Codeine phosphate group 0.03 12 ip 36.87s 196%
As can be seen from Table 1, medication group and normal saline group compare its R value greater than 130%, illustrate that oral liquid for clearing away lung-heat has antitussive action.The oral liquid for clearing away lung-heat high dose group is compared with low dose group, is caused that by ammonia the time of mouse cough prolongs to some extent, but with the codeine group relatively, the medication group causes that by ammonia the time ratio codeine group of mouse cough will lack.This shows that oral liquid for clearing away lung-heat not only has cough suppressing effect, but also have certain dose-effect relationship.
(2) the analgesic experimentation of oral liquid for clearing away lung-heat
1, material
1.1 equipment clinical thermometer, conical flask, test tube, dropper, syringe, No. five injection needles, people's catheter.
1.2 reagent escherichia coli endotoxin (O
111B
4) (the Shanghai institute of Biological Products is produced, lot number 970615).
1.3 be subjected to reagent thing oral liquid for clearing away lung-heat (medicine is made up of Herba Ephedrae (processed), Semen Armeniacae Amarum, Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Radix Salviae Miltiorrhizae, Rhizoma Bistortae, Bombyx Batryticatus, Rhizoma Polygoni Cuspidati), every dose is made concentration is 100% oral liquid 90ml (first Pharmacy department of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine provides), normal saline.
1.4 the animal rabbit (body weight 1.5～2.5Kg, do not limit, healthy anosis by male and female.Provide by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center).
2, experimental technique and result
2.1 selected rabbit is surveyed body temperature 1～2 time every day, for three days on end more than, make rabbit adapt to survey the body temperature operation, select body temperature at 38.6～39.5 ℃, the height fluctuation 0.3 ℃ with interior person, random assortment is in each group of experiment, 5 every group, totally 5 groups.
2.2 selected rabbit is divided into 5 groups by the basal body temperature stratified random, first group is the blank group, second group is lung heat clearing low dose group (12.5g/Kg bw), the 3rd group is dosage group in the lung heat clearing (25g/Kg bw), the 4th group is lung heat clearing high dose group (50g/Kg.bw), and the 5th group is aspirin group (100mg/Kg bw).
2.3 one of quantitative endotoxin standard pipe is got in the preparation of endotoxin solution, is diluted to several heating dose concentrations, this experiment 5ug/ml with the injection normal saline.
2.4 second and third, four groups of catheters of choosing respectively pour into oral liquid for clearing away lung-heat 12.5g/Kg bw, 25g/Kg bw, 50g/Kg bw to the rabbit gastric, give with the volume normal saline for first group, irritate stomach aspirin 100mg/Kg bw for the 5th group.1 hour every rabbit is all from auricular vein injection escherichia coli endotoxin 1ml/Kg after administration.Attack the back in endotoxin and respectively surveyed body temperature once in 1.2.3.4.5.6 hour, calculate the variation of each minute body temperature, as vertical coordinate, with time is abscissa, changes with each measuring point body temperature and makes comprehensive statistics formulation temperature variation curve (body temperature measuring point and the body temperature changing value of every rabbit see the following form).Gained result such as following table
The body temperature of every rabbit of table 2 is measured and the body temperature changing value
Group
Each rabbit basal body temperature (℃) Mean body temperature rising value (Δ ℃)
Each organizes the rabbit label
Time (hour)
1 2 3 4 5 1 2 3 4 5 6
Blank 39.1 38.9 38.9 39 39.2 0.9 0.95 1.1 1.2 1.1 0.9
Lung heat clearing low 39.2 39 38.9 39 38.9 0.85 0.9 1.05 1.1 0.8 0.6
In the lung heat clearing 38.8 38.7 38.6 38.7 38.9 0.8 0.85 0.9 0.95 0.75 0.55
Lung heat clearing is high by 38.9 38.8 38.9 38.7 38.8 0.9 0.95 1 1.05 0.9 0.7
Aspirin 38.6 38.8 38.9 38.7 38.8 0.65 0.8 0.9 0.85 0.65 0.55
As can be seen from Table 2, rabbit body temperature begins to rise behind the quiet notes endotoxin, peak to about the 4th hour fervescence, after this descend gradually, normal saline group temperature curve is the pattern of fever of endotoxin heating in this experiment, its body temperature of oral liquid for clearing away lung-heat low dose group and oral liquid for clearing away lung-heat high dose group change not as dosage group in the oral liquid for clearing away lung-heat and aspirin group obvious.And the dosage group is poorer slightly than aspirin group in the oral liquid for clearing away lung-heat.Oral liquid for clearing away lung-heat has certain refrigeration function.
(3) the oral liquid for clearing away lung-heat experimentation of reducing phlegm
1. material
1.1 animal adult strain Kunming mouse (body weight 18～22g is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center).
1.2 instrument 722 spectrophotometers (production of Shanghai the 3rd analytical tool factory), general surgery apparatus one cover.
1.3 reagent phenol red solution 5g/ml (being dissolved in the normal saline) (commercially available), normal saline (taking from the Nanjing University of Traditional Chinese Medicine first Affiliated Hospital pharmacy), sodium hydroxide 1mol/L (commercially available), ammonium chloride (commercially available).
1.4 be subjected to reagent thing oral liquid for clearing away lung-heat (medicine is made up of Herba Ephedrae (processed), Semen Armeniacae Amarum, Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Radix Salviae Miltiorrhizae, Rhizoma Bistortae, Bombyx Batryticatus, Rhizoma Polygoni Cuspidati), every dose is made for 100% oral liquid 90ml.(first Pharmacy department of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine provides).
2. method and result
2.1 mice is divided into four groups at random by body weight, 10 every group.First group gavages with the volume normal saline, and second group gavages oral liquid for clearing away lung-heat 20g/Kg bw, and the 3rd group gavages oral liquid for clearing away lung-heat 40g/Kg bw, and the 4th group gavages ammonium chloride 1g/Kg bw.
2.2 half an hour behind the gastric infusion, the phenol red 0.1ml of lumbar injection (5mg)/10g bw injects phenol red back and puts to death mice half an hour, peels off the trachea surrounding tissue, cut from thyroid cartilage down to trachea bifurcation trachea, putting into the test tube that the 2ml normal saline is housed, add the 0.1ml sodium hydroxide again, is that 546nm surveys the OD value with 722 spectrophotometer wavelength, with phenol red work one standard curve, according to the phenol red content of curve calculation, compare gained result such as following table with matched group:
Table 3 oral liquid for clearing away lung-heat is to the influence of the phenol red excretion amount of mice trachea section
Group dosage (g/kg bw) the phenol red excretion amount of number of animals route of administration (ug/ml)
Blank 20ml 10 ig 0.176 ± 0.026
20 10 ig 0.766 ± 0.014 are hanged down in lung heat clearing
High 40 10 ig 1.209 ± 0.056 of lung heat clearing
Ammonium chloride 1 10 ig 1.093 ± 0.037
Oral liquid for clearing away lung-heat low dosage, high dose group and normal saline group compare as can be seen by above result, its phenol red excretion amount is all apparently higher than the normal saline group, do the t check, P＜0.05, significant difference has been described, and compare there was no significant difference P＞0.05 with the ammonium chloride group, and prove that oral liquid for clearing away lung-heat has the phenol red excretion amount of the trachea section of increasing, promote the effect of expectoration.Embodiment 4, to Immune Effects research
(1) induces the research of interleukin II effect
1.1 main material
1.1.1 be subjected to reagent thing oral liquid for clearing away lung-heat (medicine is made up of Herba Ephedrae (processed), Semen Armeniacae Amarum, Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Radix Salviae Miltiorrhizae, Rhizoma Bistortae, Bombyx Batryticatus, Rhizoma Polygoni Cuspidati).Every dose is made concentration is 100% oral liquid 90ml, (first Pharmacy department of Affiliated Hospital of Nanjing University of Traditional Chinese Medicine provides).
1.1.2 cytokine and dependent cell strain rIL-2, CTLL-2 (providing) by Nanjing military medicine institute.
1.1.3 reagent PHA, MTT (U.S. FCAG company product), dimethyl sulfoxide, lymphocyte separation medium.
1.1.4 method and result
Get 2 units of fresh blood leukocyte, add the lymphocyte separation medium isolated lymphocytes, transfer cell to 2 * 10 with culture fluid
6Individual/ml.The MTT colorimetric analysis detects the active unit of IL-2, adopts and replaces by the dmso solution first moon, and the method for introducing with reference to Gillis is calculated by IL-2 active unit.
The collaborative PHA of various dose oral liquid for clearing away lung-heat induces and induces separately the observation of IL-2:
Oral liquid for clearing away lung-heat is diluted to 10mg/ml, 5mg/ml, 2.5mg/ml, 1.25mg/ml, 5 variable concentrations of 0.625mg/ml (down together) with adding 1640 liquid that contain 6%AB serum, and the final concentration of PHA is 0.2mg/ml.
Get 24 well culture plates, adding mixes up the lymphocyte suspension of concentration and the oral liquid for clearing away lung-heat and the PHA of variable concentrations, and another culture plate adds the mixed liquor of variable concentrations oral liquid for clearing away lung-heat and PHA, the rearmounted 37 ℃ of 5%CO of mixing
2Cultivated 48 hours in the incubator, collect supernatant, detect the active unit of IL-2 respectively.The results are shown in following table:
Table 4 oral liquid for clearing away lung-heat induces the effect of IL-2
Be subjected to reagent thing oral liquid for clearing away lung-heat oral liquid for clearing away lung-heat+PHA PHA
Concentration (mg/ml) 0.625 1.25 2.5 5 10 0.625 1.25 2.5 5 10 0.2
IL-2 content (IU/L) 0.236 1.76 1.54 2.76 4.69 8.5 9.7 64.6 95.9 17.4 30.95
As can be seen from Table 4: the collaborative PHA of various dose oral liquid for clearing away lung-heat induces the result of IL-2, and it is the highest to induce tiring of IL-2 with the collaborative PHA of 2.5mg/ml～5mg/ml concentration especially.And than the independent IL-2 content 30.95IU/L height that induces of PHA.The various dose oral liquid for clearing away lung-heat induces the result of IL-2 separately, and inducing tiring of IL-2 with 5mg/ml concentration especially is height.To induce IL-2 separately be effective to oral liquid for clearing away lung-heat on the whole, but the tiring to be starkly lower than with PHA is collaborative and lure the survivor of the IL-2 that induces.
(2) research of inducement interferon effect
2.1 main material
2.1.1 cell becomes fiber (L929) cell, new castle disease virus (NDV, hemagglutinative titer 1280u/ml) stomatitis virus (VSV, TCID with viral mice
50Be 10
-6) (providing by Nanjing Medical University)
2.1.2 culture fluid cell culture fluid 90%RPM-1640 liquid contains 10% calf serum and adds multi-resistance, cell maintenance medium 95%RPM-1640 liquid contains 5% calf serum and adds multi-resistance.
2.1.3 animal ICR mice (providing) by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center
2.2 method and result
2.2.1 assay method adopts micro-cytopathic-effect inhibition assay.
2.2.2 the effect of oral liquid for clearing away lung-heat inducement interferon in the mice body and the type of interferon:
Get 18 of ICR mices, be divided into 3 groups at random, 6 every group, difference lumbar injection oral liquid for clearing away lung-heat (2g/ml), NDV and each 0.5ml of normal saline.The aseptic blood of getting after 24 hours detects interferon and tires behind the separation of serum, the results are shown in Table 5.
Table 5 oral liquid for clearing away lung-heat lures the effect of inducement interferon in the mice body
Group example number dosage interferon is tired
(log4u/ml)
Oral liquid for clearing away lung-heat 6 0.5 3.50
NDV 6 0.5 7.35
Normal saline 6 0.5 1.67
The mice serum that lumbar injection was got after 24 hours was used pancreatin (Trypsin 0.25mg/ml) or 56 ℃ of water-bath effects respectively 1 hour, or pH2.0,4 ℃ handle and measure it again after 24 hours and tire, and the results are shown in Table 6.
The type of table 6 oral liquid for clearing away lung-heat inducement interferon
Treatment IFNtiter(log2u/ml)
Nnne 7.0
Trypsin 1hr 3.4
56℃Water 1hr 4.7
pH2.0?4℃ 24hr 4.4
Tiring of oral liquid for clearing away lung-heat inducement interferon is 3.50log4u/ml as can be seen from Table 5, and the normal saline group induces to tire and is 1.67log4u/ml, compare for two groups, there were significant differences (P＜0.05), illustrate that oral liquid for clearing away lung-heat has the effect of inducement interferon, but the interferon that oral liquid for clearing away lung-heat induces is tired then lower with comparing of inducing of NDV, the ability that the oral liquid for clearing away lung-heat inducement interferon is described than NDV a little less than.It tires and obviously descends the interferon that induces of oral liquid for clearing away lung-heat after 1 hour through the pancreatin effect as can be seen from Table 6, it is tired and also descends obviously after 24 hours and through 4 ℃ of effects of 56 ℃ of water-bath effects 1 hour or pH2.0, show that the interferon that oral liquid for clearing away lung-heat induces is responsive to 56 ℃ of water-baths and pH2.0 effect, the character that meets gamma interferon (INF-γ) is described.
Embodiment 5, clinical research and result of study
We have carried out relevant clinical case in first Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Changzhou institute of traditional Chinese medicine, Yancheng City institute of traditional Chinese medicine and have observed.We use oral liquid for clearing away lung-heat (4～12 months each 20ml, 1
+～3 years old each 30ml, 3
+～6 years old each 40ml, 3 times on the equal 1st, oral) treatment infantile viral pneumonia syndrome of phlegm-heat obstructing lung 104 examples, use ribavirin injection (by 10mg/kg every day simultaneously, add in 10% liquid of glucose intravenous drip, 1 time on the 1st) compare (having observed 43 examples altogether), carry out controlled clinical trial research, 10 days is 1 course of treatment.The result shows that total effects compares: in test group 104 examples, and 83 examples of fully recovering, produce effects 13 examples, effective 6 examples, invalid 2 examples, the recovery from illness obvious effective rate is 92.3%; In matched group 43 examples, 26 examples of fully recovering, produce effects 7 examples, effective 6 examples, invalid 4 examples.The recovery from illness obvious effective rate is 76.7%.The test group curative effect obviously is better than matched group (P＜0.05).Test group disappears and all obviously is better than matched group aspect the routine number at disappear routine number and extinction time, pulmonary rale of temperature recovery normal time, asthma.Test group 104 examples do not find all that during treating infant has any untoward reaction, and treatment back check routine blood test, routine urinalysis, stool routine examination, electrocardiogram, hepatic and renal function there is no abnormal change.
Oral liquid for clearing away lung-heat clinical trial model case
1 of example * *, man, August.On June 16th, 1998 went to a doctor.Hospitalized in 2 days because of cough heating and to be admitted to hospital.Admission number 94308.Infant heating when being admitted to hospital, the time low when high, cough is acute, apasm of coughing, larynx has rale, the thick difficulty of expectorant is coughed up, and asthma is arranged slightly, lip and nail are dark purple not, does not have vomiting, thirstyly do not show, the urine yellow skin, stool is normal, appetite owes to shake, red tongue, yellow and thin fur, fingerprint is purple to stagnate, and is positioned at FENGGUAN.Have a medical check-up: T:38.3 ℃, P:130 time/minute, R:35 time/minute, pharyngeal hyperemia, auscultation; Two lung audibles and wheezing sound and middle fine bubbling rale.White minute WBC12.6 * 10 of having a blood test
9/ L, N:0.26, L:0.60, M:0.14.CRP＜10mg/L。Throat swab is cultivated (-), and Respirovirus detects: the respiratory syncytial virus positive.Full rabat: bottom right lung mottling.Diagnosis: viral pneumonia.Dialectical: syndrome of phlegm-heat obstructing lung.Promptly give oral liquid for clearing away lung-heat 20ml/ time after being admitted to hospital, oral, every day 3 times.Generate heat after treating 2 days and move back, asthma disappears, and coughing after 5 days alleviates, and gurgling with sputum disappears after 9 days, the disappearance of coughing up phlegm of coughing after 11 days.Auscultation of lung is the rale disappearance after 11 days, and check blood divides WBC:8.7 * 10 in vain
9/ L, L:0.62, N:0.33, M:0.05.Full rabat: pulmonary lesions absorbs, and recovery from illness is left hospital.
Example is 2 old * *, man, 2 years old.On March 8th, 1998 went to a doctor.Hospitalized in 2 days because of fever and cough asthma and to be admitted to hospital.Admission number: 90891.Infant heating, paroxysm of cough when being admitted to hospital, it coughs acute, gurgling with sputum, the asthma flaring of nares is obvious, and lip and nail are dark purple not, does not tell, and stool is transferred, the urine yellow skin, stomach is received and is owed to shake, red tongue, yellow and greasy fur, fingerprint is purple to stagnate, and sees FENGGUAN.Have a medical check-up: 39.5 ℃ of T, P 120 times/minute, R 40 times/minute, pharyngeal hyperemia, auscultation two lung audibles and rhonchi and middle fine bubbling rale.Lab testing: blood divides in vain: WBC:4.7 * 10
9/ L, N:0.29, L:0.65, M:0.06.Routine urinalysis (-).Stool routine examination (-).CRP＜10mg/L。Full rabat shows: twice enhanced lung markings, see mottling.Throat swab is cultivated (-), and Respirovirus detects: adenovirus 3,7 types.Diagnosis: viral pneumonia.Dialectical: syndrome of phlegm-heat obstructing lung.Promptly give oral liquid for clearing away lung-heat 30ml/ time after being admitted to hospital, every day 3 times is oral.Treating generated heat after 2 days moves back, and the asthma cyanosis disappears after 3 days, and the cough rale alleviates, disappears after 8 days after 4 days, and pulmonary rale disappears after 6 days in treatment, continue the treatment of clothes oral liquid for clearing away lung-heat after 9 days all symptoms all disappear.Checking full rabat shows; Two lung marking are many slightly, and focus absorbs.Blood divides in vain: WBC:8.4 * 10
9/ L, N:0.21, L:0.75, M:0.04.The recovery from illness in 9 days of being in hospital is altogether left hospital.
Claims (7)
1, a kind of oral liquid for clearing away lung-heat for the treatment of infantile viral pneumonia is characterized in that it is to be made by following materials of weight proportions medicine: sweet moxibustion Herba Ephedrae 3～5g, Semen Armeniacae Amarum 8～12g, Gypsum Fibrosum 20～40g, Semen Lepidii (Semen Descurainiae) 5～10g, sweet moxibustion Cortex Mori 8～12g, Radix Peucedani 8～12g, system Bombyx Batryticatus 5～8g, Radix Salviae Miltiorrhizae 5～8g, Rhizoma Polygoni Cuspidati 10～15g, Rhizoma Bistortae 10～15g.
2, oral liquid for clearing away lung-heat preparation according to claim 1 is characterized in that also being added with adjuvant sucrose 6～8g.
3, a kind of method for preparing claim 1 oral liquid for clearing away lung-heat, its preparation process is:
(1) weigh and distill after Semen Armeniacae Amarum 8～12g pulverizes, collect its distillate, standby;
(2) Herba Ephedrae (processed), Gypsum Fibrosum, Semen Lepidii (Semen Descurainiae), Cortex Mori, Radix Peucedani, Radix Salviae Miltiorrhizae, Rhizoma Bistortae, Bombyx Batryticatus, Rhizoma Polygoni Cuspidati 9 flavor medicines and the Semen Armeniacae Amarum medicinal residues with described weight proportion merge back decocting secondary, after the secondary medicinal liquid merges, concentrate, after adding ethanol, staticly settle the leaching supernatant, reclaim ethanol to there not being the alcohol flavor, it is an amount of to add water, boils back cold preservation, with standby behind the extracting liquid filtering;
(3) getting sucrose 6～8g, to add water an amount of, and heating for dissolving is prepared into sucrose solution, filters, standby;
(4) merge drug extract and sucrose solutions such as Semen Armeniacae Amarum distillate, Herba Ephedrae, add water move to 90ml, packing, sterilization 30 minutes under the circulation steam and make oral liquid.
4, the preparation method of oral liquid for clearing away lung-heat according to claim 3, it is characterized in that at step (2) decocting secondary, for the first time add water 5-10 and doubly measure, merceration decocted 1～1.5 hour after 30～60 minutes, for the second time adding water 8-10 doubly measures, decocted 0.5～1 hour, the secondary medicinal liquid merges the back and be concentrated into relative density 1.02-1.15 under 60～80 ℃, adds ethanol and reaches 60～70% to containing the alcohol amount, precipitate 24～36 hours, boiling the back cold preservation time is 24～48 hours.
5, a kind of oral liquid for clearing away lung-heat for the treatment of infantile viral pneumonia is characterized in that it can also be made by following materials of weight proportions medicine: sweet moxibustion Herba Ephedrae 3～5g, Semen Armeniacae Amarum 8～12g, Gypsum Fibrosum 20～40g, Semen Lepidii (Semen Descurainiae) 5～10g, sweet moxibustion Cortex Mori 8～12g, Radix Peucedani 8～12g, Rhizoma Polygoni Cuspidati 10～15g, Radix Scutellariae 6～10g.
6, a kind of method for preparing claim 5 oral liquid for clearing away lung-heat, its preparation process is:
(1) weigh Herba Ephedrae (processed), Gypsum Fibrosum, Semen Armeniacae Amarum, Semen Lepidii (Semen Descurainiae), Radix Scutellariae, Cortex Mori, Radix Peucedani, Rhizoma Polygoni Cuspidati, decoct with water secondary, 10 times of water gagings decocted 1.5 hours for the first time, and 8 times of water gagings decocted 1.0 hours for the second time;
(2) twice decocting liquid merged the back and filter, it is 1.05～1.08 that filtrate decompression is concentrated into relative density, puts to be chilled to below 40 ℃;
(3) slowly add ethanol and make and contain alcohol amount and reach 70%, fully stir, left standstill 12 hours;
(4) filter, decompression filtrate recycling ethanol adds water to 85ml to there not being the alcohol flavor, boils 10 minutes, leaves standstill 12 hours;
(5) filter, filtrate adds stevioside, sucrose, potassium sorbate, and adds water to 90ml, stirs evenly, filtration, embedding, and sterilization makes oral liquid for clearing away lung-heat.
7, the preparation method of oral liquid for clearing away lung-heat according to claim 6 is characterized in that the stevioside that adds is 1-2g, sucrose 5-15g, potassium sorbate 0.1-0.3g in step (5).
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