CN1201670C

CN1201670C - Detoxicated pencillim dregs, its preparing process and application of its somatic protein in feed

Info

Publication number: CN1201670C
Application number: CNB011063807A
Authority: CN
Inventors: 孙玉胜; 马兰
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-03-27
Filing date: 2001-03-27
Publication date: 2005-05-18
Anticipated expiration: 2021-03-27
Also published as: CN1376401A

Abstract

The present invention relates to detoxification penicillin bacterium dregs and a preparation method thereof and an application as mycoprotein in feed, wherein the detoxification process and the drying process of the penicillin bacterium dregs are completed through technical steps of strain preparation, pretreatment, fermentation in a fermentation tank, spray drying, etc. The preparation method has the advantages of reasonable production technology design, easy operation and stable product quality; the preparation method is suitable for large-scale industrial production, solves the problems of difficult thorough detoxification, difficult drying processing, low feeding safety, environment pollution, etc. of the penicillin bacterium dregs and opens up a practical path for a sustainable development strategy to realize the clean production of penicillin fermentation industry.

Description

Detoxification penicillin mushroom dregs and preparation method thereof and as the application of mycoprotein in feed

The present invention relates to a kind of detoxification penicillin mushroom dregs and preparation method thereof and with it as the application of mycoprotein in feed.

According to incompletely statistics, China's annual output penicillin mushroom dregs is given money as a gift to count and is reached more than ten thousand tons, the analysis showed that, penicillin mushroom dregs contains and accounts for the protein of dry weight more than 40%, abundant vitamin, nutriments such as growth factor and culture medium residue, also and depositing the various metabolites of microorganism, under poisonous and harmful substances such as handling additive is arranged, as phenylacetic acid, penicillic acid, dipotassium amine, formaldehyde, polyacrylamide etc.For many years, this resource is regarded as industrial residue always and abandons, and causes a large amount of wastings of resources and environmental pollution.In recent years, the domestic producer that has adopts the method for removing water treatment and heated-air drying with the penicillin mushroom dregs convection drying, produce animal feeding-stuff containing somatic protein, though this has solved the pollution of waste bacterial slag to environment, but this method can only be destroyed the part poisonous and harmful substance, and detoxification is not thorough, and feeding security is low, addition also is restricted, it also is a problem that feed detects up to standard, also a nearly period, in the penicillin production process, because bacterial classification, the difference that process tank is criticized, the physical behavior of bacterium slag is changed to some extent, and self-dissolving makes bacterium refuse lac bulk properties outstanding as thalline, and viscosity is big, above-mentioned drying process is not easy to operate, cause the energy consumption height, treating capacity is low, degradation problem under the nutritional quality, especially treating capacity is low causes a large amount of bacterium slags to can not get in time handling and being dropped, and the wasting of resources and problem of environmental pollution are given prominence to once again.

The object of the present invention is to provide a kind of detoxification penicillin mushroom dregs, this bacterium slag nutritional quality is good, and steady quality has no side effect.

Another object of the present invention is to provide a kind of preparation method of detoxification penicillin mushroom dregs, this method can be improved the physical behavior of bacterium slag simultaneously with the thorough detoxification of penicillin mushroom dregs, just its suitable spray-dired method is carried out the drying processing, thereby the raising treating capacity cuts down the consumption of energy, and improves product quality.

A further object of the present invention provides a kind ofly to be used as mycoprotein the detoxification penicillin mushroom dregs in feed.

The object of the present invention is achieved like this:

1. the preparation of bacterial classification: the bacterial classification that uses is brewer's yeast (S.cerevisiae Hansen) 2.1190 and red pseudomonas (Rhodopseudomonas), in the two compound cultivation liquid medium within, two bacterial classifications provide by the Applied Microbiology Inst., Heilongjiang Academy of Sciences, and medium component is: carbon source material 1-5%; Ammonium sulfate 0.1-2.0%; Dipotassium hydrogen phosphate 0.01-1.0%; Calcium carbonate 0.001-0.01; Sodium chloride 0.01-0.1%; Yeast extract 0.1-3.0%; Condition of culture, 15-35 ℃ of intermittently ventilation, throughput 1: 0.4-1: 0.8vvm intermittently stirs, speed of agitator 100-220rpm, cultivation cycle 1-5 days, mature seed was a weak yellow liquid, PH3-5.

2, pre-treatment: the penicillin wet bacteria slag is added acid solution, and example hydrochloric acid or other acid solution are transferred PH2-5, handle 4-8 hour for 60 ℃-80 ℃, are neutralized to PH6.0-6.5 with alkali lye such as sodium hydroxide solution or other alkali lye.

3, ferment tank: the wet bacteria slag after the above-mentioned processing is gone in the fermentation tank, charge accounts for the 60%-80% of fermentation volume, and other adds carbon source material such as glucose or other glucide that accounts for bacterium slag weight 1%-10%, sterilizes 30 minutes for 121 ℃, when treating that temperature is reduced to 30 ℃ ± 2 ℃, insert the seed liquor of 0.5%-25%, at 15 ℃-35 ℃, intermittently ventilation, throughput 1: 0.4-1: 0.8vvm, intermittently stir, speed of agitator 100-220rpm cultivated 2-7 days down, put zymotic fluid and went in the basin.

4, spray-drying: control import humidity at 160 ℃-190 ℃, 70 ℃-80 ℃ of outlet temperatures.Collect dry powder and be detoxification penicillin mushroom dregs finished product.It is characterized by the yellowish-brown powdery, fragrance slightly, moisture 9%-12%; Protein 40%-50%; Coarse ash 3%-10%; Calcium 1.0%-1.5%; Phosphorus 0.8%-1.2%.

The present invention directly utilizes wet penicillin mushroom dregs to carry out detoxification treatment, has solved the halfway problem of penicillin mushroom dregs detoxification on the one hand, has also solved the bacterium slag on the other hand and has been difficult for dry problem, and this resource can be fully utilized, and avoids its pollution to environment.And production technology is reasonable in design, and processing ease is fit to large-scale industrial production, has high economic benefit and far-reaching ecological benefits.

Fig. 1 is penicillin mushroom dregs detoxification drying technology process figure

Embodiment 1

10 tons of penicillin wet bacteria slags are squeezed into process tank processing industry hcl acidifying to PH2-4, handled 4-8 hour for 60-80 ℃, with in the industrial sodium hydroxide solution and PH6.0-6.5, change in the fermentation tank, add 100 kilograms of glucose again, sterilized 30 minutes, and be cooled to about 30 ℃ for 121 ℃, insert the seed liquor of 2%-3%, in 30 ℃ ± 2 ℃, intermittently ventilation, throughput 1: 0.5-1: 0.8vvm, speed of agitator 150rpm, cultivated 4-6 days, and treated that putting jar after fermentation broth viscosity reduces carried out spray-drying, its inlet temperature is controlled at 160-190 ℃, outlet temperature is controlled at 70-80 ℃, collects dry powder and is detoxification penicillin mushroom dregs finished product.

Application Example 1

The application of detoxification penicillin mushroom dregs in the grow-finish pig feed.

Test group feed formula: corn flour 62%; Wheat bran 20%; Soya-bean cake 4%; Detoxification penicillin mushroom dregs 12%; Lysine 0.24%; Bone meal 0.79%; Oyster shell whiting 0.74%; Salt 0.23%;

Trophic level: crude protein 15.91%; Calcium 0.6%; Phosphorus 0.5%; Salt 0.23%; Lysine 0.75%; Methionine+cystine 0.58%; Digestible energy 13.84 million Jiao/kilograms.

The control group feed formula is: honest 808 20% concentration pig fodders, manufacture by description of product proportioning.

Test with pig is: Du Luoke * army herds cenospecies No. 1, average starting weight 20.4kg, each 60 random packet of test group and control group, similarity condition was raised 100 days, the average end of test group weighs 96.85 kilograms/head, and 3.58: 1 average ends of control group of feedstuff-meat ratio weigh 95.91 kilograms/head, feedstuff-meat ratio 3.69: 1.

Application Example 2

The application of detoxification penicillin mushroom dregs in broiler fodder.

Test group feed formula: corn flour 62%; Dregs of beans 28%; Detoxification penicillin mushroom dregs 6%; Grease 1%; Lysine 0.13%; Methionine 0.14%; Vitamin 0.01%; Trace element 0.1%; Tricalcium phosphate 1.5%; Salt 0.3%; Stone flour 0.82%; Trophic level: crude protein 20.51%; Lysine 1.09%; Methionine 0.45%; Calcium 0.70%; Phosphorus 0.67%; Metabolizable energy 12.16 million Jiao/kilograms.

The control group feed formula: the vast fryer in Harbin is with hyperconcetration material No. 2 4%; Corn flour 64%; Dregs of beans 30%; Grease 2%;

Test is 2 ages in week of Ai Wei bacterium with fryer, derives from the school graduates of Heilongjiang Province chicken house, and test group and control group respectively are 500, random packet, and the raising condition is identical, and deliver for sale seven ages in week.Control group has 4 death, and experimental group has 2 death.The average end of control group weighs 2.35 kilograms/, and feedstuff-meat ratio is 2.04: 1; The average end of test group weighs 2.38 kilograms/, and feedstuff-meat ratio is 2.02: 1.

Claims

1. detoxification penicillin mushroom dregs, it is characterized in that: this detoxification penicillin mushroom dregs is that the penicillin mushroom dregs that produces when producing with penicillin fermentation is a raw material, removing noxious material after fermentation liquid by inoculation brewer's yeast and red pseudomonas fermentation is prepared from through super-dry, its physical behavior of finished product is the yellowish-brown powdery, slightly fragrance contains moisture 9%-12%; Protein 40%-50%; Coarse ash 3%-10%; Calcium 1.0%-1.5%; Phosphorus 0.8%-1.2%.

2. the preparation method of a detoxification penicillin mushroom dregs, it is characterized in that: this method is finished by following processing step:

(1) preparation of bacterial classification: the bacterial classification that uses is brewer's yeast (S.cerevisiae Hansen) 2.1190 and red pseudomonas (Rhodopseudomonas), in the two compound cultivation liquid medium within, two bacterial classifications provide by the Applied Microbiology Inst., Heilongjiang Academy of Sciences, and medium component is: carbon source material 1-5%; Ammonium sulfate 0.1-2.0%; Dipotassium hydrogen phosphate 0.01-1.0%; Calcium carbonate 0.001-0.01; Sodium chloride 0.01-0.1%; Yeast extract 0.1-3.0%; Condition of culture: 15-35 ℃, intermittently ventilation, throughput 1: 0.4-1: 0.8vvm intermittently stirs, speed of agitator 100-220rpm, cultivation cycle 1-5 days, mature seed was a weak yellow liquid, pH value 3-5;

(2) pre-treatment: malicious penicillin mushroom dregs is added acid solution transfer pH value 2-5, handled 4-8 hour for 60-80 ℃, use in the alkali lye and pH value 6.0-6.5;

(3) ferment tank: the wet bacteria slag after the above-mentioned processing is gone in the fermentation tank, charge accounts for the 60-80% of fermentation volume, and other adds the carbon source material that accounts for bacterium slag weight 1-10%, sterilizes 30 minutes for 121 ℃, when treating that temperature is reduced to 30 ± 2 ℃, insert the seed liquor of 0.5-25%, at 15-35 ℃, intermittently ventilation, throughput 1: 0.4-1: 0.8vvm, intermittently stir, speed of agitator 100-220rpm cultivated 2-7 days down, put zymotic fluid to go in the storage tank;

(4) spray-drying: the control inlet temperature outlet temperature 70-80 ℃, is collected dry powder and is detoxification penicillin mushroom dregs finished product at 160-190 ℃.

3. the preparation method of detoxification penicillin mushroom dregs according to claim 2 is characterized in that: the acid solution among the above-mentioned preparation technology is meant the corresponding aqueous solution of technical-grade hydrochloric acid, sulfuric acid, acetic acid.

4. the preparation method of detoxification penicillin mushroom dregs according to claim 2 is characterized in that: the alkali lye among the above-mentioned preparation technology is meant the corresponding aqueous solution of technical grade NaOH, potassium hydroxide, sodium carbonate, sodium acid carbonate, quick lime, ammonia.

5. the preparation method of detoxification penicillin mushroom dregs according to claim 2 is characterized in that: the carbon source material among the preparation technology is meant glucose, molasses, sucrose, maltose, maltose, dextrin, starch.

6. detoxification penicillin mushroom dregs according to claim 1 is characterized in that: this detoxification penicillin mushroom dregs is used in feed as mycoprotein.