CN1200004C - Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted - Google Patents
Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted Download PDFInfo
- Publication number
- CN1200004C CN1200004C CN 03114898 CN03114898A CN1200004C CN 1200004 C CN1200004 C CN 1200004C CN 03114898 CN03114898 CN 03114898 CN 03114898 A CN03114898 A CN 03114898A CN 1200004 C CN1200004 C CN 1200004C
- Authority
- CN
- China
- Prior art keywords
- tea
- polysaccharide
- extracted
- polyphenol
- tankage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000004676 glycans Chemical class 0.000 title claims abstract description 36
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 36
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 36
- 241001122767 Theaceae Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 11
- 150000008442 polyphenolic compounds Chemical class 0.000 title abstract 3
- 235000013824 polyphenols Nutrition 0.000 title abstract 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 12
- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 claims abstract description 12
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 229960000278 theophylline Drugs 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 108010059820 Polygalacturonase Proteins 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 4
- 235000013619 trace mineral Nutrition 0.000 abstract description 2
- 239000011573 trace mineral Substances 0.000 abstract description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 229910052749 magnesium Inorganic materials 0.000 abstract 1
- 239000011777 magnesium Substances 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 150000008267 fucoses Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 238000010010 raising Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention belongs to a method for extracting tea polysaccharide from waste tea which is extracted. The waste tea from which tea polyphenol is extracted has main components of tea polysaccharide, tea polyphenol, theophylline, protein, and trace elements of calcium, ferrum, magnesium, etc. The content of total sugar is from 10% to 15%. Toxic theophylline and a majority of protein are extracted by chloroform, and the waste tea which is extracted is then dried. The coarse polysaccharide (about 35% to 50% of sugar content) can be obtained. The technology of the present invention is simple and practical, and an extraction yield can reach more than 40%. The present invention has low cost and is suitable for large-scale production of enterprises.
Description
Technical field
The present invention relates to a kind of method of from the tankage that extracted tea-polyphenol, extracting tea polysaccharide.
Background technology
The natural high moleculer eompound that tea polysaccharide is made up of monose such as glucose, pectinose, ribose wood sugar, semi-lactosi, Fucoses, molecular-weight average is about 4 * 10
4Dalton, tea polysaccharide also should comprise multiple small molecules oligose.That tea polysaccharide has is hypoglycemic, reducing blood-fat, strengthening immunity, atherosclerosis, anticoagulation, antithrombotic effect.Though it is various that the extraction tea polysaccharide technology of having reported from tealeaves has, major part all has following main step:
Tea-leaf power---→ hot water dissolving---→ centrifugal the insoluble impurities that removes---→ must contain the clear liquid of polysaccharide---→ remove albumen with Sevag method or additive method---3-5 times of contained polysaccharide of volume alcohol precipitation of → adding---→ polysaccharide that is precipitated out is further purified purelyr polysaccharide.
Use traditional method and extract tea polysaccharide, though raw material sources are abundant, it is low to extract yield, because of alcohol precipitation only works to macromolecular polysaccharide, has the oligomeric tea polysaccharide of small molecules of drug action not have precipitatedly to go out to stay in the solution in addition in a large number.Expend a large amount of energy and alcohol simultaneously, increased production cost.
Summary of the invention
The purpose of this invention is to provide a kind of method of from the tankage that extracted tea-polyphenol, extracting tea polysaccharide.
It is with behind the extraction tea-polyphenol, the green powder tankage of sugar degree 10-15% are as raw material, water logging bubble dissolving polysaccharide with 7-10 times of mass ratio, again with 40 ℃-60 ℃ effects of 1%-10% polygalacturonase 2-3 hour, remove insoluble impurities with 3000-4000 rev/min of centrifuging then, add the chloroform extraction theophylline and the foreign protein of 2-8 times of volume again, standing demix, get supernatant fulvescent liquid, vacuum-drying promptly gets sugar degree 35%-50% Crude polysaccharides.
Advantage of the present invention:
1) the tankage past behind the tealeaves extraction tea-polyphenol is not utilized, and existing is raw material with it, turns waste into wealth, and has improved the tealeaves utilization ratio.
2) this technology has been removed this step of alcohol precipitation, except also having activated small molecules oligomeric polysaccharide, macromolecular polysaccharide also kept basically like this, loss seldom, so just improved the extraction yield of tea polysaccharide, up to about 40%, compare with traditional technology and to have improved tens times of raisings.
3) the macromole tea polysaccharide is a kind of protein-contg polysaccharide, owing to removed this step of alcohol precipitation, the gained active polysaccharide is influenced very little.
4) avoided this conventional procedures of alcohol precipitation, whole technological process is simple, and less energy consumption has greatly been saved production cost.
5) this patent adopts chloroform extraction to eliminate toxic substance theophylline in the tealeaves, and removes a certain amount of foreign protein.
6) adopt this patent method can also keep in the tealeaves other human body is had the activeconstituents tea-polyphenol of drug action and various trace elements etc.
Embodiment
The present invention extracts tea polysaccharide and oligomeric polysaccharide from tankage, adopt polygalacturonase to remove pectin, adopts chloroform extraction to remove theophylline and foreign protein, and then directly goes vacuum-drying.
Embodiment 1:
Get 300 gram tankage powder, with 50 degree left and right sides warm water dissolvings, the volume of water is 2100ml.The water-bath of after the dissolving liquid glucose being put into about 40 degree temperature adds the 1% polygalacturonase effect of measuring 2 hours, then with the 3000 centrifugal removal insoluble substances of right speed that turn left, the chloroform that adds 2 times of volumes again in surplus liquid stirs extraction, leave standstill the chloroform layer of emitting the bottom after 30 minutes, the brown solution of residue transparent clear is drying to obtain tea polysaccharide and oligomeric polysaccharide with Vacuumdrier.
Embodiment 2:
Get 500 gram tankage powder, with 55 degree warm water dissolvings, the volume of water is 4500ml.The water-bath of after the dissolving liquid glucose being put into about 50 degree temperature adds the 5% polygalacturonase effect of measuring 2.5 hours, then with the 3500 centrifugal removal insoluble substances of right speed that turn left, the chloroform that adds 5 times of volumes again in surplus liquid stirs extraction, leave standstill the chloroform layer of emitting the bottom after 30 minutes, the brown solution of residue transparent clear is drying to obtain tea polysaccharide and oligomeric polysaccharide with Vacuumdrier.
Embodiment 3:
Get 800 gram tankage powder, with 55 degree warm water dissolvings, the volume of water is 8000ml.The water-bath of after the dissolving liquid glucose being put into about 60 degree temperature adds the 10% polygalacturonase effect of measuring 3 hours, then with the 4000 centrifugal removal insoluble substances of right speed that turn left, the chloroform that adds 8 times of volumes again in surplus liquid stirs extraction, leave standstill the chloroform layer of emitting the bottom after 30 minutes, the brown solution of residue transparent clear is drying to obtain tea polysaccharide and oligomeric polysaccharide with Vacuumdrier.
Claims (1)
1. method of from the tankage that extracted tea-polyphenol, extracting tea polysaccharide, it is characterized in that, after will extracting tea-polyphenol, the green powder tankage of sugar degree 10%-15% are as raw material, water logging bubble dissolving polysaccharide with 7-10 times of mass ratio, again with 40 ℃-60 ℃ effects of 1%-10% polygalacturonase 2-3 hour, remove insoluble impurities with 3000-4000 rev/min of rotating speed centrifuging then, the chloroform extraction theophylline and the foreign protein that add 2-8 times of volume again, standing demix, get supernatant fulvescent liquid, vacuum-drying promptly gets sugar degree 35-50% Crude polysaccharides.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03114898 CN1200004C (en) | 2003-01-13 | 2003-01-13 | Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03114898 CN1200004C (en) | 2003-01-13 | 2003-01-13 | Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1431226A CN1431226A (en) | 2003-07-23 |
| CN1200004C true CN1200004C (en) | 2005-05-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03114898 Expired - Fee Related CN1200004C (en) | 2003-01-13 | 2003-01-13 | Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1200004C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213997B (en) * | 2008-01-04 | 2011-07-27 | 上海师范大学 | Leisure health tea beverage and preparation thereof |
| CN104045732A (en) * | 2014-07-03 | 2014-09-17 | 王健 | Extraction method of tea polysaccharide |
| CN109320629A (en) * | 2018-12-13 | 2019-02-12 | 成都华高生物制品有限公司 | A kind of extracting method of tea polysaccharide |
-
2003
- 2003-01-13 CN CN 03114898 patent/CN1200004C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1431226A (en) | 2003-07-23 |
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Granted publication date: 20050504 Termination date: 20100222 |