CN104045732A - Extraction method of tea polysaccharide - Google Patents
Extraction method of tea polysaccharide Download PDFInfo
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- CN104045732A CN104045732A CN201410315458.6A CN201410315458A CN104045732A CN 104045732 A CN104045732 A CN 104045732A CN 201410315458 A CN201410315458 A CN 201410315458A CN 104045732 A CN104045732 A CN 104045732A
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- tea
- tea polysaccharide
- polysaccharide
- warm water
- extracting
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an extraction method of tea polysaccharide. The extraction method comprises the steps of: with branches and leaves trimmed in a tea production process or tea wastes generated in a tea processing process as raw materials, smashing the raw materials, then, transferring the smashed raw materials into an extraction tank, extracting in 70-DEG C warm water for 30-60min, filtering, and extracting filtrate; concentrating the extracting solution at the temperature of 50 DEG C and in vacuum; settling by using 3-time industrial alcohol, and centrifugally separating to obtain a sediment, i.e., crude tea polysaccharide; dissolving the crude tea polysaccharide by using warm water, adding a mixed solution of chloroform and n-butanol in the volume ratio of 4 to 1, after stirring for 30min, standing for layering, and centrifugally separating to remove the sediment; then, settling by using industrial alcohol, and drying the sediment under the condition of vacuum freezing to obtain tea polysaccharide. Therefore, the cost is reduced, and the efficiency is increased.
Description
Technical field
The present invention relates to the extracting method of tea polysaccharide, relate in particular to the method for extracting tea polysaccharide from Tea Production process in the tea waste in a kind of branches and leaves of pruning or Tea Processing process.
Background technology
Tea polysaccharide has good pharmacological effect: radiation protection, and anticoagulation and anti thrombotic action, hypoglycemic activity, enhancing body immunologic function, but people are by drinking tea to take in tea polysaccharide at present, and the tea polysaccharide content of every cup of tea is atomic, is difficult to reach pharmacological effect.
Summary of the invention
Object of the present invention provides a kind of extracting method of tea polysaccharide, solves one or more in above-mentioned prior art problem.
According to an aspect of the present invention, provide a kind of extracting method of tea polysaccharide, its raw material is the tea waste in branches and leaves or the Tea Processing process of pruning in Tea Production process, comprises the following steps:
1. will after raw material pulverizing, proceed in extractor, lixiviate 30-60min in the warm water of 70 DEG C, filters, and extracts filtrate.
2. extracting solution is concentrated under 40-60 DEG C, vacuum.
3. by the industrial spirit precipitation of 3 times, centrifugation, obtains the thick tea polysaccharide of throw out.
4. thick tea polysaccharide is dissolved with warm water, and adding volume ratio is the chloroform propyl carbinol mixed solution of 4:1, stir after 30min, and stratification, throw out is gone out in centrifugation.
5. use industrial alcohol precipitation, throw out is dry under vacuum freezing, obtains tea polysaccharide again.
The present invention, by extract tea polysaccharide in the tea waste in branches and leaves or the Tea Processing process of pruning from Tea Production process, has reduced cost, has improved efficiency.
Embodiment
An extracting method for tea polysaccharide, its raw material is the tea waste in branches and leaves or the Tea Processing process of pruning in Tea Production process, will after raw material pulverizing, proceed in extractor, lixiviate 30-60min in the warm water of 70 DEG C, filters, and extracts filtrate.Extracting solution is concentrated under 50 DEG C, vacuum.By the industrial spirit precipitation of 3 times, centrifugation, obtains the thick tea polysaccharide of throw out.Thick tea polysaccharide is dissolved with warm water, and adding volume ratio is the chloroform propyl carbinol mixed solution of 4:1, stir after 30min, and stratification, throw out is gone out in centrifugation.Use industrial alcohol precipitation, throw out is dry under vacuum freezing, obtains tea polysaccharide again.
In sum, the present invention, by extract tea polysaccharide in the tea waste in branches and leaves or the Tea Processing process of pruning from Tea Production process, has reduced cost, has improved efficiency.
The above is only one embodiment of the present invention; it should be pointed out that to those skilled in the art, without departing from the concept of the premise of the invention; can also make some similar distortion and improvement, within these also should be considered as protection scope of the present invention.
Claims (1)
1. an extracting method for tea polysaccharide, its raw material is the tea waste in branches and leaves or the Tea Processing process of pruning in Tea Production process, it is characterized in that: comprise the following steps:
1. will after raw material pulverizing, proceed in extractor, lixiviate 30-60min in the warm water of 70 DEG C, filters, and extracts filtrate.
2. extracting solution is concentrated under 40-60 DEG C, vacuum.
3. by the industrial spirit precipitation of 3 times, centrifugation, obtains the thick tea polysaccharide of throw out.
4. thick tea polysaccharide is dissolved with warm water, and adding volume ratio is the chloroform propyl carbinol mixed solution of 4:1, stir after 30min, and stratification, throw out is gone out in centrifugation.
5. use industrial alcohol precipitation, throw out is dry under vacuum freezing, obtains tea polysaccharide again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410315458.6A CN104045732A (en) | 2014-07-03 | 2014-07-03 | Extraction method of tea polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410315458.6A CN104045732A (en) | 2014-07-03 | 2014-07-03 | Extraction method of tea polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104045732A true CN104045732A (en) | 2014-09-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410315458.6A Pending CN104045732A (en) | 2014-07-03 | 2014-07-03 | Extraction method of tea polysaccharide |
Country Status (1)
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| CN (1) | CN104045732A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107573429A (en) * | 2016-11-21 | 2018-01-12 | 广西大学 | A kind of method that jasmine tea tea polysaccharide is extracted using the precipitation method |
| CN109650349A (en) * | 2018-12-29 | 2019-04-19 | 华南农业大学 | The nanometer selenium for preparing the method for nanometer selenium using camellia plant polyose and being prepared |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1431226A (en) * | 2003-01-13 | 2003-07-23 | 浙江大学 | Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted |
| KR100602841B1 (en) * | 2005-02-28 | 2006-07-19 | 고려대학교 산학협력단 | Acidic polysaccharide with cell-binding inhibition activity isolated from green tea and its composition for preventing and treating gastrointestinal diseases |
| CN102887962A (en) * | 2012-10-10 | 2013-01-23 | 安徽工程大学 | Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide |
-
2014
- 2014-07-03 CN CN201410315458.6A patent/CN104045732A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1431226A (en) * | 2003-01-13 | 2003-07-23 | 浙江大学 | Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted |
| KR100602841B1 (en) * | 2005-02-28 | 2006-07-19 | 고려대학교 산학협력단 | Acidic polysaccharide with cell-binding inhibition activity isolated from green tea and its composition for preventing and treating gastrointestinal diseases |
| CN102887962A (en) * | 2012-10-10 | 2013-01-23 | 安徽工程大学 | Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide |
Non-Patent Citations (2)
| Title |
|---|
| 刘自力: "《林产化工产品生产技术》", 31 May 2005, article "茶多糖", pages: 336-337 * |
| 唐玉章: "林产药物的提取技术及应用（上）", 《生意通》, no. 5, 15 May 2009 (2009-05-15), pages 114 - 115 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107573429A (en) * | 2016-11-21 | 2018-01-12 | 广西大学 | A kind of method that jasmine tea tea polysaccharide is extracted using the precipitation method |
| CN109650349A (en) * | 2018-12-29 | 2019-04-19 | 华南农业大学 | The nanometer selenium for preparing the method for nanometer selenium using camellia plant polyose and being prepared |
| CN109650349B (en) * | 2018-12-29 | 2022-08-12 | 华南农业大学 | Method for preparing nano-selenium by utilizing camellia plant polysaccharide and prepared nano-selenium |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140917 |