CN119317710A - Rna聚合酶变体 - Google Patents

Rna聚合酶变体 Download PDF

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Publication number
CN119317710A
CN119317710A CN202380039861.8A CN202380039861A CN119317710A CN 119317710 A CN119317710 A CN 119317710A CN 202380039861 A CN202380039861 A CN 202380039861A CN 119317710 A CN119317710 A CN 119317710A
Authority
CN
China
Prior art keywords
amino acid
rna polymerase
rna
ome
cap
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380039861.8A
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English (en)
Chinese (zh)
Inventor
埃米·E·拉比多
玛格丽特·富兰克林
阿萨纳西奥斯·杜西斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ModernaTx Inc
Original Assignee
ModernaTx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ModernaTx Inc filed Critical ModernaTx Inc
Publication of CN119317710A publication Critical patent/CN119317710A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202380039861.8A 2022-04-14 2023-04-13 Rna聚合酶变体 Pending CN119317710A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263331145P 2022-04-14 2022-04-14
US63/331,145 2022-04-14
PCT/US2023/065719 WO2023201294A1 (en) 2022-04-14 2023-04-13 Rna polymerase variants

Publications (1)

Publication Number Publication Date
CN119317710A true CN119317710A (zh) 2025-01-14

Family

ID=86329119

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380039861.8A Pending CN119317710A (zh) 2022-04-14 2023-04-13 Rna聚合酶变体

Country Status (5)

Country Link
US (1) US20250250551A1 (enExample)
EP (1) EP4508201A1 (enExample)
JP (1) JP2025513874A (enExample)
CN (1) CN119317710A (enExample)
WO (1) WO2023201294A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3134131T3 (en) 2014-04-23 2022-02-07 Modernatx Inc Nucleic acid vaccines
WO2017020026A1 (en) 2015-07-30 2017-02-02 Modernatx, Inc. Concatemeric peptide epitopes rnas
CN108430456B (zh) 2015-10-22 2022-01-18 摩登纳特斯有限公司 癌症疫苗
MD3718565T2 (ro) 2015-10-22 2022-09-30 Modernatx Inc Vaccinuri împotriva virusului respirator
JP6983455B2 (ja) 2016-09-14 2021-12-17 モデルナティーエックス， インコーポレイテッド 高純度ｒｎａ組成物及びその調製のための方法
CN110167587A (zh) 2016-11-11 2019-08-23 摩登纳特斯有限公司 流感疫苗
WO2018170270A1 (en) 2017-03-15 2018-09-20 Modernatx, Inc. Varicella zoster virus (vzv) vaccine
CN111212905A (zh) 2017-08-18 2020-05-29 摩登纳特斯有限公司 Rna聚合酶变体
WO2019148101A1 (en) 2018-01-29 2019-08-01 Modernatx, Inc. Rsv rna vaccines
AU2019345067A1 (en) 2018-09-19 2021-04-08 Modernatx, Inc. High-purity peg lipids and uses thereof
US12151029B2 (en) 2018-09-19 2024-11-26 Modernatx, Inc. PEG lipids and uses thereof
CN113874502A (zh) 2019-03-11 2021-12-31 摩登纳特斯有限公司 补料分批体外转录方法
WO2022150717A1 (en) 2021-01-11 2022-07-14 Modernatx, Inc. Seasonal rna influenza virus vaccines
US20220363937A1 (en) 2021-05-14 2022-11-17 Armstrong World Industries, Inc. Stabilization of antimicrobial coatings
CN118373866A (zh) * 2023-01-20 2024-07-23 深圳瑞吉生物科技有限公司 一种用于核酸的5’端加帽的化合物及其应用
US20250270523A1 (en) * 2023-03-01 2025-08-28 Nanjing Vazyme Biotech Co., Ltd. Rna polymerase variant, and preparation method therefor and use thereof in rna synthesis
CN120060188A (zh) * 2023-11-29 2025-05-30 武汉核圣生物技术有限公司 T7-rna聚合酶突变体及其应用
WO2025152833A1 (zh) * 2024-01-19 2025-07-24 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用
CN120775820A (zh) * 2024-04-07 2025-10-14 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体
CN118389495B (zh) * 2024-06-24 2024-10-01 北京悦康科创医药科技股份有限公司 一种核糖修饰的加帽类似物及其应用
EP4650358A3 (en) 2024-04-26 2026-01-14 Beijing Youcare Kechuang Pharmaceutical Technology Co., Ltd. Ribose-modified cap analog and use thereof
WO2026089040A1 (ja) * 2024-10-25 2026-04-30 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体
CN120330158B (zh) * 2025-06-19 2025-09-12 南京诺唯赞生物科技股份有限公司 Rna聚合酶变体及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL215513B1 (pl) 2008-06-06 2013-12-31 Univ Warszawski Nowe boranofosforanowe analogi dinukleotydów, ich zastosowanie, czasteczka RNA, sposób otrzymywania RNA oraz sposób otrzymywania peptydów lub bialka
EP2377938A1 (en) * 2010-04-16 2011-10-19 Eukarys Capping-prone RNA polymerase enzymes and their applications
WO2017066797A1 (en) 2015-10-16 2017-04-20 Modernatx, Inc. Trinucleotide mrna cap analogs
JP2022521094A (ja) * 2019-02-20 2022-04-05 モデルナティエックス インコーポレイテッド 共転写キャッピング用ｒｎａポリメラーゼバリアント

Also Published As

Publication number Publication date
WO2023201294A1 (en) 2023-10-19
EP4508201A1 (en) 2025-02-19
JP2025513874A (ja) 2025-04-30
US20250250551A1 (en) 2025-08-07

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